Made available in DSpace on 2016-06-02T19:32:10Z (GMT). No. of bitstreams: 1 6089.pdf: 2493882 bytes, checksum: b18fa0ec5dde486ef33533f0babf7c86 (MD5) Previous issue date: 2014-03-26 / Universidade Federal de Sao Carlos / The fall armyworm Spodoptera frugiperda (SMITH, 1797) is a voracious pest of numerous crops of economic importance throughout the New World. In Brazil, their larvae can be attacked by several species of parasitic wasps, which are candidate to biological control agents against this pest. Surveying the parasitoid fauna on S. frugiperda in maize throughout Brazil two very morphological similar species of Campoletis are found. To ensure the efficiency of the biological control works, the knowledge about the taxonomy of the species related is necessary. Campoletis specimens emerged from Spodoptera frugiperda were studied mofologically and molecularly, in order to solve taxonomic problems among C. sonorensis (CAMERON, 1886) and C. flavicincta (ASHMEAD,1890). The samples came from three regions of Brazil: Santa Maria, RS; Ribeirão Preto, SP; Sete Lagoas, MG. Pictures from the type material of the two species were studied, besides the original descriptions of them. A possible sinonimy is indicated between the both species, for which the name C. sonorensis will be used based on the priority law for species names. A redescription is made to Campoletis sonorensis from Brazil, using morphological characters and DNA Barcoding. / Spodoptera frugiperda (SMITH, 1797) é uma praga voraz de diversas culturas de importância econômica no Novo Mundo. No Brasil, suas larvas podem ser atacadas por diversas espécies de vespas parasitóides que são candidatos a agentes de controle biológico contra essa praga. Pesquisando os parasitóides da fauna de Spodoptera frugiperda em cultivos de milho no Brasil, duas espécies do gênero Campoletis, muito similares morfologicamente, foram encontradas. Para garantir a eficiência dos trabalhos de controle biológico, é de grande importância o conhecimento taxonômico das espécies relacionadas. Exemplares de Campoletis emergidos de Spodoptera frugiperda foram estudados morfológica e molecularmente, a fim de resolver os problemas taxonômicos existentes entre as espécies C. sonorensis (CAMERON, 1886) e C. flavicincta (ASHMEAD,1890). Os exemplares foram provenientes de três regiões do Brasil: Santa Maria, RS; Ribeirão Preto, SP; Sete Lagoas, MG. Fotos de material tipo das duas espécies foram estudadas, além das suas descrições originais. Os resultados indicam uma possível sinonímia entre as duas espécies, para a qual será usado o nome C. sonorensis com base na lei da prioridade para o nome das espécies. Apresentamos uma redescrição para Campoletis sonorensis usando caracteres morfológicos e de DNA Barcoding.

The presented thesis deals with the issue of tourism in the European Union with emphasis on its economic importance, deployment and current trends that can be identified in this sector. Its aim is to assess the issue of tourism in the EU from a different perspective than it is usual in the literature devoted to this topic, and try to find answers to several questions, including whether the economic importance of tourism is really as great within the EU as it is often attributed to this sector, or how tourism is deployed in the EU. The basis for answering these and other questions is the analysis of a number of indicators over the last two decades. In terms of current trends in tourism, attention is paid to trends in demand and supply and the issue of sustainable tourism development.

Objetivo: Realizou-se um estudo, no período de 2002 a 2009, com a finalidade de verificar se as mudanças nas classes de cobertura da terra e no uso do solo podem exercer influência na ocorrência da raiva, nos municípios de Jacareí e de Santa Branca, situados na Região do Vale do Paraíba, no Estado de São Paulo, Sudeste do Brasil. Métodos: A ferramenta utilizada para avaliar estas alterações foi o sensoriamento remoto, através de imagens de satélite Land-sat. Para pesquisar a presença do vírus da raiva (RABV) foram coletados animais silvestres atropelados nas rodovias, morcegos encontrados na área urbana em atitude suspeita, morcegos hematófagos da espécie Desmodus rotundus e animais de interesse econômico (ADIE) que vieram a óbito por enfermidade com sintomatologia nervosa. O material coletado, sistema nervoso central (SNC), desses animais foi encaminhado para o laboratório de referência nacional, o Instituto Pasteur de São Paulo (IP-SP) e para o Laboratório de Sanidade Animal da Agência Paulista de Tecnologia dos Agronegócios (APTA)- Pólo Regional do Vale Paraíba (PRDTA/VP) - Pindamonhangaba-SP. A determinação da presença do antígeno viral foi feita através da prova de imunofluorescência direta (IFD) e o isolamento do vírus através da inoculação intracerebral em camundongos (IC). Os inóculos das amostras foram submetidos à prova de reação em cadeia pela polimerase transcriptase reversa (RT-PCR) para verificar a presença do vírus da raiva. Resultados: No período de estudo tivemos a ocorrência de dez casos da doença, sendo três em morcegos insetívoros na zona urbana de Jacareí, e sete em ADIE, sendo dois, em Jacareí e cinco, em Santa Branca. Todos os animais silvestres terrestres examinados foram negativos para a presença do vírus da raiva em todas as provas realizadas (IFD, IC, RT-PCR). O material proveniente dos morcegos foi negativo para as provas de IFD e IC, porém três amostras, oriundas de morcegos insetívoros, resultaram positivas para a prova de RTPCR. As amostras de ADIE examinadas resultaram positivas para todas as provas realizadas. O sequenciamento genético utilizou amplificações referentes à glicoproteína viral das 8 amostras positivas para rt-pcr, obtendo para os cinco isolados de ADIE linhagem de desmodus rotundus e para os três isolados de morcegos linhagens de nyctinomops laticaudatus e tadarida brasiliensis. Quando foi estudada a cobertura do solo e seu uso, considerando os municípios, constatou-se que não haviam ocorrido mudanças significativas entre 2002 e 2009. Optou-se por fazer buffers de raio de 3 km tendo como centro de cada buffer as coordenadas geográficas de casos positivos para raiva. Como em duas situações houve sobreposição entre áreas de buffers, resolveu-se considerar a área da união dos mesmos. Foi interessante que, mesmo nestes buffers não houve mudanças significativas, apesar da ocorrência da doença. Conclusão: Na escala utilizada, considerada micro, a enfermidade aconteceu, mesmo sem mudanças aparentes na região. Provavelmente, em uma escala macro outros resultados poderiam ser obtidos / Objective: This study was carried out during the period of 2002-2009, with the purpose to verify whether the changes in types of land cover and in land use can influence the occurrence of rabies in Jacareí and Santa Branca, situated in the Vale do Paraíba, State of São Paulo, southeastern brazil. Methods: The tool used to evaluate these alterations was the remote sensing through images of satellite land-sat. In order to search for the presence of rabies virus, brain materials were collected from wild animals roadkilled in highways, bats found in the urban area in suspicious attitude, desmodus rotundus hematophagous bats captured in rural areas and farm animals of economic importance (aei) dead with suspect of rabies. For rabies diagnosis, specimens of central nervous system (cns) of these animals were sent to the Pasteur Institute of São Paulo (IP-SP) - national reference laboratory and to the laboratory of animal Paulista Agency for Agribusiness Technology (APTA) - Polo Valley Regional of Paraíba (PRDTA / VP), in Pindamonhangaba, state of SP. The presence of viral antigen was determined by the direct fluorescent antibody technique (D-FAT) and the isolation of the virus by means of intracerebal mouse inoculation test (MIT). The brain suspensions were submitted to reverse transcriptase. Polymerase chain reaction to check the presence of rabies virus. Results: In the period of study, 10 positive cases were detected, being 3 in insectivorous bats in the urbane zone of jacareí, and 7 in aei, being 2 in jacareí and 5 in santa branca. All the terrestrial wild animals examined were negative for the presence of rabies virus in all tests performed (d-fat, mit, and rt-pcr). The materials from bats were found negative for the d-fat and mit, however, three samples originating from insectivorous bats turned out to be positive for rt-pcr. The positive samples from aei were positive for all tests carried out. The genetic sequencing of the g gene of eight rt-pcr positive rabies virus isolates derived from aie, five corresponded to the desmodus rotundus lineage and the three bat isolates were related to lineages of nyctinomops laticaudatus and tadarida brasiliensis. When land coverage and its use were analyzed, considering the municipalities, it was found that no significant changes occurred between 2002 and 2009. Then buffers of 3 km in radius were chosen taking as the center of each buffer the geographical coordinates of positive rabies cases. Since in two situations there was superposition between areas of buffers, it was resolved to consider the junction area of the buffers. Even in these buffers there was no significant change, despite the occurrence of the disease. Conclusion: it was concluded that the disease has occurred even without any apparent changes in the region, due to the (micro) scale used in this study. Probably, in a macro scale, other results might be obtained

Objetivo: Realizou-se um estudo, no período de 2002 a 2009, com a finalidade de verificar se as mudanças nas classes de cobertura da terra e no uso do solo podem exercer influência na ocorrência da raiva, nos municípios de Jacareí e de Santa Branca, situados na Região do Vale do Paraíba, no Estado de São Paulo, Sudeste do Brasil. Métodos: A ferramenta utilizada para avaliar estas alterações foi o sensoriamento remoto, através de imagens de satélite Land-sat. Para pesquisar a presença do vírus da raiva (RABV) foram coletados animais silvestres atropelados nas rodovias, morcegos encontrados na área urbana em atitude suspeita, morcegos hematófagos da espécie Desmodus rotundus e animais de interesse econômico (ADIE) que vieram a óbito por enfermidade com sintomatologia nervosa. O material coletado, sistema nervoso central (SNC), desses animais foi encaminhado para o laboratório de referência nacional, o Instituto Pasteur de São Paulo (IP-SP) e para o Laboratório de Sanidade Animal da Agência Paulista de Tecnologia dos Agronegócios (APTA)- Pólo Regional do Vale Paraíba (PRDTA/VP) - Pindamonhangaba-SP. A determinação da presença do antígeno viral foi feita através da prova de imunofluorescência direta (IFD) e o isolamento do vírus através da inoculação intracerebral em camundongos (IC). Os inóculos das amostras foram submetidos à prova de reação em cadeia pela polimerase transcriptase reversa (RT-PCR) para verificar a presença do vírus da raiva. Resultados: No período de estudo tivemos a ocorrência de dez casos da doença, sendo três em morcegos insetívoros na zona urbana de Jacareí, e sete em ADIE, sendo dois, em Jacareí e cinco, em Santa Branca. Todos os animais silvestres terrestres examinados foram negativos para a presença do vírus da raiva em todas as provas realizadas (IFD, IC, RT-PCR). O material proveniente dos morcegos foi negativo para as provas de IFD e IC, porém três amostras, oriundas de morcegos insetívoros, resultaram positivas para a prova de RTPCR. As amostras de ADIE examinadas resultaram positivas para todas as provas realizadas. O sequenciamento genético utilizou amplificações referentes à glicoproteína viral das 8 amostras positivas para rt-pcr, obtendo para os cinco isolados de ADIE linhagem de desmodus rotundus e para os três isolados de morcegos linhagens de nyctinomops laticaudatus e tadarida brasiliensis. Quando foi estudada a cobertura do solo e seu uso, considerando os municípios, constatou-se que não haviam ocorrido mudanças significativas entre 2002 e 2009. Optou-se por fazer buffers de raio de 3 km tendo como centro de cada buffer as coordenadas geográficas de casos positivos para raiva. Como em duas situações houve sobreposição entre áreas de buffers, resolveu-se considerar a área da união dos mesmos. Foi interessante que, mesmo nestes buffers não houve mudanças significativas, apesar da ocorrência da doença. Conclusão: Na escala utilizada, considerada micro, a enfermidade aconteceu, mesmo sem mudanças aparentes na região. Provavelmente, em uma escala macro outros resultados poderiam ser obtidos / Objective: This study was carried out during the period of 2002-2009, with the purpose to verify whether the changes in types of land cover and in land use can influence the occurrence of rabies in Jacareí and Santa Branca, situated in the Vale do Paraíba, State of São Paulo, southeastern brazil. Methods: The tool used to evaluate these alterations was the remote sensing through images of satellite land-sat. In order to search for the presence of rabies virus, brain materials were collected from wild animals roadkilled in highways, bats found in the urban area in suspicious attitude, desmodus rotundus hematophagous bats captured in rural areas and farm animals of economic importance (aei) dead with suspect of rabies. For rabies diagnosis, specimens of central nervous system (cns) of these animals were sent to the Pasteur Institute of São Paulo (IP-SP) - national reference laboratory and to the laboratory of animal Paulista Agency for Agribusiness Technology (APTA) - Polo Valley Regional of Paraíba (PRDTA / VP), in Pindamonhangaba, state of SP. The presence of viral antigen was determined by the direct fluorescent antibody technique (D-FAT) and the isolation of the virus by means of intracerebal mouse inoculation test (MIT). The brain suspensions were submitted to reverse transcriptase. Polymerase chain reaction to check the presence of rabies virus. Results: In the period of study, 10 positive cases were detected, being 3 in insectivorous bats in the urbane zone of jacareí, and 7 in aei, being 2 in jacareí and 5 in santa branca. All the terrestrial wild animals examined were negative for the presence of rabies virus in all tests performed (d-fat, mit, and rt-pcr). The materials from bats were found negative for the d-fat and mit, however, three samples originating from insectivorous bats turned out to be positive for rt-pcr. The positive samples from aei were positive for all tests carried out. The genetic sequencing of the g gene of eight rt-pcr positive rabies virus isolates derived from aie, five corresponded to the desmodus rotundus lineage and the three bat isolates were related to lineages of nyctinomops laticaudatus and tadarida brasiliensis. When land coverage and its use were analyzed, considering the municipalities, it was found that no significant changes occurred between 2002 and 2009. Then buffers of 3 km in radius were chosen taking as the center of each buffer the geographical coordinates of positive rabies cases. Since in two situations there was superposition between areas of buffers, it was resolved to consider the junction area of the buffers. Even in these buffers there was no significant change, despite the occurrence of the disease. Conclusion: it was concluded that the disease has occurred even without any apparent changes in the region, due to the (micro) scale used in this study. Probably, in a macro scale, other results might be obtained

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No. of bitstreams: 2 Dissertação - Escleide Gomes Cabral - 2017.pdf: 6925522 bytes, checksum: 2783097ac78b5d87ec6e06310e6e7e3e (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-02-22 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Forestry is booming in Brazil due to demand for forest products. However, despite the importance of planted forests, forestry in the state of Goiás has been little studied. One of the reasons for the absence of studies in the state is due to the lack of disaggregated data and the great pulverization of the plantations, making it difficult to survey them. This work aimed to know the areas with commercial forest plantations, through remote sensing in the years 2002 and 2013, through the mapping carried out by Probio and TerraClass Cerrado, respectively, and in 2015 by the mapping carried out in this work, to assist in the Planning and development of state forestry. Analyzing the production and the values collected with the forest products in Goiás, we observed that some products have been replaced over the years by others, such as charcoal by wood chips, as well as other products that have been increasing in the production, such as firewood, which in the year 2000 obtained a production of 679,755 m³ and obtained a value of 6.6 million Reals. In less than 15 years, its production increased to 4,357,778 m³ of firewood. When we looked at the 2002, 2013 and the mapping of this study, we realized that there were important advances in the planted areas, so that in 2002 there were just over 500 ha of forest planted in Goiás; In 2013, were approximately 153 thousand ha; And in 2015, the area of forestry was 162,516 ha. The mesoregions with the largest commercial forest plantations are in the South, East and North, and the South and the East are the largest producers of wood and timber in the state of Goiás. The spatial distribution of eucalyptus plantations in Goiás Characterized by plantations in small areas and by a non-vertical production of the producers, reflecting the potential of forestry activity in income generation in small and medium farms and that despite the methodological differences used in the mappings used, the results were close to the results found for the current landscape of the state of Goiás elaborated in this work / A silvicultura está em franca expansão no Brasil devido à demanda pelos produtos florestais. No entanto, apesar da importância das florestas plantadas, a silvicultura no estado de Goiás tem sido pouco estudada. Um dos motivos para a ausência de estudos no estado deve-se à falta de dados desagregados e à grande pulverização dos plantios, dificultando o levantamento dos mesmos. Este trabalho teve como objetivo conhecer as áreas com plantios comerciais de florestas, por meio de sensoriamento remoto nos anos 2002 e 2013, através dos mapeamentos realizados pelo Probio e TerraClass Cerrado, respectivamente, e em 2015 pelo mapeamento realizando nesse trabalho, para que auxiliem no planejamento e desenvolvimento da silvicultura estadual. Analisando a produção e os valores arrecadados com os produtos florestais em Goiás, observamos que alguns produtos vêm sendo substituídos, ao longo dos anos por outros, como, por exemplo, o carvão vegetal por cavacos de madeira, assim como outros produtos que vem apresentando aumento na produção, como a lenha, que no ano 2000 obteve uma produção de 679.755 m³ e conseguiu um valor de 6,6 milhões de reais. Em menos de 15 anos, sua produção passou a ser 4.357.778 m³ de lenha. Ao examinar os mapeamentos de 2002, 2013 e o elaborado neste estudo, percebemos que houve importantes avanços nas áreas plantadas, de forma que, em 2002, havia pouco mais de 500 ha de floresta plantada em Goiás; em 2013, foram aproximadamente 153 mil ha; e, em 2015, a área de silvicultura foi de 162.516 ha. As mesorregiões com as maiores áreas plantio comercial de florestas são a Sul, Leste e Norte, sendo que a Sul e a Leste são as maiores produtoras de lenha e madeira em tora no estado de Goiás. A distribuição espacial dos plantios de eucalipto em Goiás se caracteriza por plantios em pequenas áreas e por uma produção não verticalizada dos produtores, refletindo o potencial da atividade florestal na geração de renda em pequenas e médias propriedades e que apesar das diferenças de metodológicas utilizadas nos mapeamentos utilizados, os resultados foram próximos dos resultados encontrados para a paisagem atual do estado de Goiás elaborada neste trabalho.

Darbo tikslas- įvertinti karvių bandos sergamumą mastitais, prieš įdiegiant automatizuotą melžimo sistemą ir po įdiegimo, kaip keitėsi pieno sudėties, kokybės rodikliai, bei ištirti pieno elektrini laidumą. Tyrimas atliktas ūkyje „x“ 2011- 2013 metų laikotarpyje. Surinkti pieno SLS, BBS, riebalų, baltymų, laktozės, urėjos duomenys iš 3-6m. amžiaus laktuojančių karvių melžiant į liniją ir pradėjus melžti „Lely Astronaut“ melžimo robotu, bei surinkti AMS duomenys apie pieno EL. Pieno sudėties bei kokybės tyrimai atlikti VĮ “Pieno tyrimai” laboratorijoje. Nustatyti dažniausi karvių mastito sukėlėjai ūkyje. / The objective of this work is to assess the incidences of mastitis in the herd and to evaluate milk composition, quality indicators, and the milk electrical conductivity before and after an installation of an automated milking system. The study was conducted in the farm "x" during the year period from 2011 to 2013. During this study, milk SCC, TBC, fat, protein, lactose, and urea data of lactating cows from 3 to 6 years old was collected. The milk was firstly collected by using a pipeline milking system and later by using a “Lely Astronaut” robotic milking system (AMS). Data about the milk electric conductivity was collected from the AMS. Milk composition and quality research was performed by the State Enterprise “Pieno tyrimai” laboratory. The most common causes of mastitis were identified in the farm.

Currently the gold mining industry is taxed differently to other industries. It is taxed on a two-tier system. The nature of the gold mining tax formula encourages the mining of marginal gold ores. Firms that are involved in the mining of gold are subjected to a "tax tunnel", which is a tax free revenue portion. This is against the equity principle of taxation because it separates companies on the basis of what they produce and not on the basis of income generated. The South African government is in the process of implementing a revenue-based royalty system. The majority of firms in the gold mining industry feel that for the benefit of economic growth the government must consider implementing a profit-based royalty system. This study analyses the gold mining tax formula in comparison to the flat rate tax. It also analyses the reasons for the differential treatment of the gold mining industry. / Economics / M.Comm.

Currently the gold mining industry is taxed differently to other industries. It is taxed on a two-tier system. The nature of the gold mining tax formula encourages the mining of marginal gold ores. Firms that are involved in the mining of gold are subjected to a "tax tunnel", which is a tax free revenue portion. This is against the equity principle of taxation because it separates companies on the basis of what they produce and not on the basis of income generated. The South African government is in the process of implementing a revenue-based royalty system. The majority of firms in the gold mining industry feel that for the benefit of economic growth the government must consider implementing a profit-based royalty system. This study analyses the gold mining tax formula in comparison to the flat rate tax. It also analyses the reasons for the differential treatment of the gold mining industry. / Economics / M.Comm.

The aim of the thesis was to evaluate primary and secondary potential of the development of tourism in the destination Hluboká nad Vltavou and to propose, based on performed analyses, possibilities of strategic development of tourism along with defining priorities and proceedings. The theoretical part of the thesis introduces the problem. The analysis that followed was made with the help of marketing research. These were the basis for SWOT analyses with the definition of strong and weak points. The problem analysis then described the main problems and barriers of the monitored area.

The aim of this thesis called The Possibilities of Strategic Development of Tourism in the Region of Písek was to evaluate primary and secondary development potential of tourism in the chosen area and on the basis of this analysis to propose the possibilities of strategic development of tourism along with stating priorities and proceedings. The theoretical part of the thesis introduces the problem. The analysis that followed was made with the help of marketing research. These were the basis for SWOT analyses with the definition of strong and weak points. The problem analysis then described the main problems and barriers of the monitored area.