Parmi les œufs de poisson, qui sont une ressource aquatique nutritionnelle intéressante, ceux de la truite arc-en-ciel (Oncorhynchus mykiss) contiennent une quantité élevée de protéines et une huile riche en acides gras polyinsaturés (AGPI), avec une proportion très importante de phospholipides. Cependant, l’œuf de poisson présente une capacité élevée d’auto-protection contre les contraintes extérieures, qui limite la destructuration de son réseau protéique par attaque enzymatique. Ainsi, le degré d’hydrolyse des œufs de la truite l’Alcalase®, la Neutrase® et la Protamex® varie entre 3 et 7 %, ce qui est très faible (20 % dans la majorité des protéines animales). L’extraction des lipides après protéolyse partielle est incomplète, probablement en raison d’interactions fortes avec les protéines faiblement hydrolysées. Ils contiennent une teneur élevée en phospholipides (53 % des lipides totaux) et les acides gras polyinsaturés entrent pour 42 % des acides gras totaux. Les AGPI, notamment le DHA, sont situés préférentiellement en position sn-2 sur la molécule de glycérol ce qui est particulièrement intéressant du point de vue nutritionnel. La stabilité à l’oxydation de l’huile a été étudiée par diverses méthodes, dont la spectrométrie infrarouge à transformée de Fourier. Cette méthode s’est avérée extrêmement intéressante pour une analyse structurale de la dégradation de l’huile en cours d’oxydation. Il peut être conclu que les lipides tirés des œufs de la truite arc-en-ciel ou de poisson en général, ont un réel avenir en matière de complément alimentaire ou nutraceutique, à condition de lever l’obstacle de l’hydrolyse enzymatique des protéines du chorion et du vitellus / Fish eggs, especially those of the rainbow trout (Oncorhynchus mykiss) in the present study, are an interesting nutritional aquatic source. They contain proteins of high value, as well as an oil rich in polyunsaturated fatty acids (PUFA) with a large percentage of phospholipids. However, they exhibit a high auto-protection capacity against environmental constraints and thus, the degree of hydrolysis of rainbow trout eggs by Alcalase®, Neutrase® and Protamex® proteases varied solely within 3-7 %. This value was low compared with the 20 % obtained in most animal proteins. The phospholipid content was high (53 % of total lipids) and PUFA accounted for 42 % of total fatty acids. Among PUFA, DHA was found preferably at the sn-2 position of the glycerol backbone, which is of special interest about nutritional properties. The oil release by enzymatic hydrolysis was found limited compared with chemical methods, probably because of the strong interactions engaged with the incomplete destructured protein network. The oxidative stability of the oil was studied through several methods in which the infrared Fourier transform appeared as the best tool for structural analysis along the oxidation process. As a conclusion, lipids from fish eggs, especially from rainbow trout, could be a nutritional breakthrough, as far the enzymatic hydrolysis of the vitellus and of the chorion proteins is achieved

This thesis deals with important factors that affect the temporal organization of emergence and early survival of Baltic salmon (Salmo salar L.). The study population was obtained from the Norrfors hatchery (63°50'N,20°05'E), Umeälven (Ume river) in Northern Sweden. The main objectives of the thesis has been to study; a: the effect of female and egg characteristics on embryonic survival, b: the effect of egg size, temperature and photoperiod on the emergence pattern and c: the impact of early or late emergence on survival in relation to predation and limited territorial space. The main results are summarized as follows: (1) Fecundity and egg size increased with increasing weight of females. No effect of female size were found on egg colour. Longer impoundment and later stripping increase egg colour. Egg mortality was not correlated with egg colour. Stripping date was found to have the strongest effect on mortality. (2) Egg size had no effect on the timing of emergence but fry of different egg size emerged synchronously. Fry from large eggs left the gravel as heavier fry and with a larger proportion of yolk left compared to fry from small eggs. (3) The number of days and number of degree days from hatching to 50% emergence decreased exponentially with increasing temperature. Synchronization of emergence increased with increasing temperature. Fry emerged with more yolk at 12 °C compared to 6 °C. (4) Eggs kept in a LD 16:8 light regime hatched mainly during the light period, while eggs kept in constant darkness hatched continously over a 24 hour period. Alevins kept at different light regimes (light>4h) from hatching until emergence left the gravel during the dark period. Daylength had no effect on the annual onset of emergence. (5) In a laboratory stream channel, predator presence at emergence increased mortality especially in early emerging fry. If the predator was introduced after completed emergence high mortality was noted among late emerging fry. The presence of fish predators and a limited territorial space for fry seemed to make early and late emergence hazardous and to favour a "peak" emergence. In the presence of a predator the fry changed their behaviour by reducing their swimming activity. / digitalisering@umu.se

M.Sc. (Zoology) / Please refer to full text to view abstract

The thermal environment exerts a strong influence on many aspectsof squamate life history. Environmental temperatures constrain daily activity time, fecundity, growth rate, and also influences hatchling phenotype. Cold nest temperatures can slow the development of eggs deposited in the soil, cause developmental abnormalities or result in mortality of embryos.

The aim of the diploma thesis was to distinguish bird species based on the expected different protein composition of eggs and their individual components using mass spectrometry. The obtained results could be possibly used to identify the animal origin of egg materials used in works of art. For the research, components of the eggs (yolk, egg white and their mixture) of nine available bird species were collected and dried - pheasant (Phasianus colchicus), domestic goose (Anser anser domesticus), domestic duck (Anas platyrhynchos domesticus), muscovy duck (Cairina moschata), chicken (Gallus gallus f. domestica) - four different specimens, Japanese quail (Coturnix japonica), pampas rhea (Rhea americana), red ore (Alectoris rufa), speckled guinea fowl (Numida meleagris). The samples were digested by the enzyme trypsin and prepared using ZIP-TIP for the next measurements by MALDI-TOF (Matrix-Assisted Laser Desorption / Ionization - Time of Flight) mass spectrometry. The obtained data were evaluated by Principal Component Analysis (PCA). The used method showed that the most bird species can be distinguished by yolks (with an approximately 95% success rate) and more than half of the species by egg white proteins (success rate 83%) and by the egg white and yolk mixtures (success rate 80%). Two samples of...

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<p> This research was designed to gain a better understanding of the relationships between nutrition, aging and oogenesis. Cohorts of 1800 inbrod, adult house flies were maintained on various undefined and chemically defined diets. From these flies samples for histology were removed periodically, and the rest were analyzed for their survival and their ability to develop and lay viable eggs. The survival of the females was more affected by nutrition and other factors than that of the males. In females fed sugar water, oogenesis was arrested at an early stage, but survival was lower than on a milk diet. From adult emergence until the completion of one or more ovarian cycles, the larval and adult fatbodies and the ovary were compared as to their histology, histochemistry and cytology. / Thesis / Master of Science (MSc)

No description available.

No description available.

In April of 1990 the Sebastian Inlet Tax District received a long-term (25 year) permit to conduct maintenance dredging of the navigation channel at Sebastian Inlet, Florida. Approximately 105,150 cubic meters of sediment will be removed every two years and pumped to a feeder beach immediately south of the inlet. The renourished beach, a high density nesting beach for loggerhead (Caretta caretta) and green (Chelonia mydas) sea turtles, is within the proposed Archie Carr National Wildlife Refuge and will play a critical role in the long-term maintenance of Sebastian Inlet as a navigable waterway. A turtle monitoring program was implemented by the District at the request of the U.S. Fish and Wildlife Service. The monitoring program was designed to investigate nesting behavior, reproductive success and physical characteristics of the nesting habitat on the renourished beach at Sebastian Inlet and on a control beach (Wabasso Beach). Daily nesting surveys were conducted to record all sea turtle nests and non-nesting emergences in each study area. All nests were marked, monitored throughout incubation and inventoried to determine hatching and emergence success. Data on physical parameters were collected in monthly profiles of each beach. Compaction values were recorded with a cone penetrometer along transects from the dune to mean high water line. Temperature of the ambient beach was recorded at 30 and 60-cm depths along two transects from the dune to mean high water line. Moisture and sand grain analysis were conducted monthly in 1991. Sea turtle nesting and hatching success were similar on the renourished and control beaches in 1990 and 1991, suggesting that renourishment had no adverse effect on sea turtle reproductive success. Nesting success on the renourished and control beaches, respectively, was 42.0% (N = 104) and 45.0% (N = 65) in 1990, and 62.9% (N = 165) and 62.4% (N = 118) in 1991. Hatching success on the renourished and control beaches, respectively, was 80.7% (N = 85) and 79.3% (N = 59) in 1990, and 69.1% (N = 118) and 66.7% (N = 102) in 1991. Compaction values averaged 500 PSI on the renourished beach, while values on the control were approximately 250 PSI. The higher level of compaction on the renourished beach resulted in a lack of complete nest covering, and aberrant nest chamber configurations on the renourished beach. Grain size and moisture content were similar on the renourished and control beaches. Compaction levels were not related to incompatible fill, implying that the manner in which the material was deposited resulted in the increased compaction. Beach temperatures measured at 30-cm and 60-cm depths indicated that the renourished beach was consistently 0.5°C warmer than the control beach. If the physical parameters of a subsequent renourishment project are within the findings of 1990 and 1991, there should be no adverse effect on sea turtle nesting and hatching success. The results of this project cannot be applied to other beach renourishment projects however, but should be restricted to future dredging projects at Sebastian Inlet. Although the results are not applicable to other locations, the methodologies developed to study the effects of renourishment on sea turtles could be applied to other beach renourishment situations. Future research should focus on how the physical parameters of a renourished beach affect the micro-environment within the actual nest chamber and embryonic development. / Master of Science