The thesis deals with molecular gastronomy as an expanding culinary direction and is focused on the use in chemistry teaching in primary and secondary education. There are described home experiments which were verified, documented and explained.

A utilização de sistemas de bateria de gaiolas é assunto de grande polêmica nos países da Europa, sendo que a maior preocupação com o uso de gaiolas se dá quanto ao espaço oferecido às aves poedeiras, o qual certamente afeta seu bem-estar. Sendo assim este trabalho teve como objetivos avaliar os aspectos relacionados ao comportamento e bem-estar das aves, comparando o sistema de criação convencional (bateria de gaiolas) com um sistema de criação em cama, ninho e poleiro, bem como avaliar a influencia das condições ambientais (estresse e conforto) na qualidade final dos ovos, associando técnicas da zootecnia de precisão (análise de imagens), para avaliar as respostas comportamentais das diferentes linhagens nas diferentes condições. Dois grupos de 20 aves (10 Hy-line W36 e 10 Hy-line Brown) em inicio de postura foram submetidas a duas condições ambientais durante duas semanas consecutivas (26°C e 60% UR e 35°C e 70%UR), sendo também submetidas a dois sistemas de criação (bateria de gaiolas e cama). Durante o período de avaliação um sistema de câmeras de vídeo registrava o comportamento das aves. Os ovos produzidos durante todo o período da pesquisa foram avaliados pela análise dos seguintes parâmetros de qualidade: peso do ovo, espessura da casca, gravidade especifica, unidades Haugh, além de análise da colorimetria da gema, porosidade da casca e análises microbiológicas, para a verificação de uma possível ocorrência de contaminação por Salmonella sp na gema e casca dos ovos. Os resultados obtidos revelaram uma redução significativa (P<0,05) nos parâmetros de qualidade do ovo para a condição de estresse térmico, principalmente para o sistema de criação em gaiolas. Quanto as análises de comportamento, o sistema de criação em cama proporcionou a expressão de todos os comportamentos naturais e de conforto das aves, favorecendo assim melhores condições de bem-estar. Para o sistema de criação convencional (gaiolas) foi possível verificar que mesmo sem nenhuma condição, as aves ainda tentavam executar seus comportamentos naturais, sendo que a impossibilidade de expressarem estes comportamentos certamente agravou a condição de estresse provocada por este sistema de criação. Pela análise dos dois sistemas de criação e das duas condições ambientais a linhagem Hy-Line W36. foi a que obteve um melhor desempenho. / The battery cage system is a very polemic issue in European countries, being the most commonly raised concerns focused on the spatial restrictions of the hens, which might compromise important comfort movements affecting their welfare conditions. This work aimed to evaluate aspects related to behavior and welfare of hens comparing a conventional housing system (laying cages in battery) with another system using litter, lair and roost. In addition, the effects of environmental conditions (stress or comfort) on final egg quality and behavior responses (analyzed by precision techniques) were evaluated in different laying hen lines. Two groups of 20 birds (10 Hyline W36 and 10 Hy-line Brown) at the beginning of production phase were submitted to two environmental conditions (26°C with 60% RU or 35°C with 70% RU) and two housing systems (cages in battery or litter) during two consecutive weeks. During the evaluation period, bird behaviors were recorded by video cameras. Total egg production was analyzed according to egg weight, shell thickness, specific gravidity, Haugh unit, yolk colorimetric parameters and shell porosity. Eggs were microbiologically analyzed in order to detect Salmonella sp contamination in yolk and eggshell. A significant (P<0.05) reduction in quality parameters was observed in eggs from laying hens raised under heat stress, mainly in those from laying cage system. In relation to behavior, the housing system in litters proportioned the expression of all natural behaviors and those related to bird comfort, which permitted to improve welfare conditions. In conventional system (laying cages) was verified that birds tried to manifest their natural behavior in spite of lacking condition for it. This impossibility for executing natural behaviors promoted an increase of stress provoked by the housing system. Analyzing both systems and environmental conditions, hens from Hy-Line W36 showed the better performances.

A utilização de sistemas de bateria de gaiolas é assunto de grande polêmica nos países da Europa, sendo que a maior preocupação com o uso de gaiolas se dá quanto ao espaço oferecido às aves poedeiras, o qual certamente afeta seu bem-estar. Sendo assim este trabalho teve como objetivos avaliar os aspectos relacionados ao comportamento e bem-estar das aves, comparando o sistema de criação convencional (bateria de gaiolas) com um sistema de criação em cama, ninho e poleiro, bem como avaliar a influencia das condições ambientais (estresse e conforto) na qualidade final dos ovos, associando técnicas da zootecnia de precisão (análise de imagens), para avaliar as respostas comportamentais das diferentes linhagens nas diferentes condições. Dois grupos de 20 aves (10 Hy-line W36 e 10 Hy-line Brown) em inicio de postura foram submetidas a duas condições ambientais durante duas semanas consecutivas (26°C e 60% UR e 35°C e 70%UR), sendo também submetidas a dois sistemas de criação (bateria de gaiolas e cama). Durante o período de avaliação um sistema de câmeras de vídeo registrava o comportamento das aves. Os ovos produzidos durante todo o período da pesquisa foram avaliados pela análise dos seguintes parâmetros de qualidade: peso do ovo, espessura da casca, gravidade especifica, unidades Haugh, além de análise da colorimetria da gema, porosidade da casca e análises microbiológicas, para a verificação de uma possível ocorrência de contaminação por Salmonella sp na gema e casca dos ovos. Os resultados obtidos revelaram uma redução significativa (P<0,05) nos parâmetros de qualidade do ovo para a condição de estresse térmico, principalmente para o sistema de criação em gaiolas. Quanto as análises de comportamento, o sistema de criação em cama proporcionou a expressão de todos os comportamentos naturais e de conforto das aves, favorecendo assim melhores condições de bem-estar. Para o sistema de criação convencional (gaiolas) foi possível verificar que mesmo sem nenhuma condição, as aves ainda tentavam executar seus comportamentos naturais, sendo que a impossibilidade de expressarem estes comportamentos certamente agravou a condição de estresse provocada por este sistema de criação. Pela análise dos dois sistemas de criação e das duas condições ambientais a linhagem Hy-Line W36. foi a que obteve um melhor desempenho. / The battery cage system is a very polemic issue in European countries, being the most commonly raised concerns focused on the spatial restrictions of the hens, which might compromise important comfort movements affecting their welfare conditions. This work aimed to evaluate aspects related to behavior and welfare of hens comparing a conventional housing system (laying cages in battery) with another system using litter, lair and roost. In addition, the effects of environmental conditions (stress or comfort) on final egg quality and behavior responses (analyzed by precision techniques) were evaluated in different laying hen lines. Two groups of 20 birds (10 Hyline W36 and 10 Hy-line Brown) at the beginning of production phase were submitted to two environmental conditions (26°C with 60% RU or 35°C with 70% RU) and two housing systems (cages in battery or litter) during two consecutive weeks. During the evaluation period, bird behaviors were recorded by video cameras. Total egg production was analyzed according to egg weight, shell thickness, specific gravidity, Haugh unit, yolk colorimetric parameters and shell porosity. Eggs were microbiologically analyzed in order to detect Salmonella sp contamination in yolk and eggshell. A significant (P<0.05) reduction in quality parameters was observed in eggs from laying hens raised under heat stress, mainly in those from laying cage system. In relation to behavior, the housing system in litters proportioned the expression of all natural behaviors and those related to bird comfort, which permitted to improve welfare conditions. In conventional system (laying cages) was verified that birds tried to manifest their natural behavior in spite of lacking condition for it. This impossibility for executing natural behaviors promoted an increase of stress provoked by the housing system. Analyzing both systems and environmental conditions, hens from Hy-Line W36 showed the better performances.

Made available in DSpace on 2016-08-15T20:31:24Z (GMT). No. of bitstreams: 1 MonikKOC_DISSERT.pdf: 637237 bytes, checksum: c04ae9e9367303da2f965ee75d79bfdd (MD5) Previous issue date: 2015-02-06 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The objective was to evaluate the influence of the addition of propolis extract in diets with different levels of crude protein on growth performance and quality of laying eggs Isa Label created in high temperature environment. For this, we used 104 birds distributed in a completely randomized design in a 2x2 factorial design (CP level and propolis extract) being four diets, one control and another with 5% reduction of crude protein, each with and without the addition of 0.70% of propolis extract with 13 repetitions and two birds each. Were evaluated: egg production rate, feed conversion, absolute and relative weight of yolk, albumen and shell, height and width of the egg, specific gravity, yolk color, eggshell thickness, Haugh unit, albumen and yolk index. Propolis extract (0.70%) provided increase of egg production rate and improves feed conversion, to the crude protein level of 17.70%, and promote coloring sharper yolk. Therefore, the level of 0.70% hydroalcoholic extract of propolis can be used in diets for laying hens Isa label as it improves desirable characteristics such as egg production rate, feed conversion and yolk color when the feed has protein level of 17 7%. And the protein level of 16.82% can be used for laying Isa Label without prejudice to on broiler performance / Objetivou-se avaliar a influência da adição de extrato hidroalcoólico de própolis em rações com dois níveis de proteína bruta, no desempenho produtivo e qualidade de ovos de poedeiras Isa Label criadas em ambiente de alta temperatura. Foram utilizadas 104 aves, distribuídas em delineamento inteiramente casualizado, esquema fatorial 2x2 (proteína bruta e extrato hidroalcoólico de própolis), sendo uma ração controle e outra com redução de 5% da proteína bruta, cada uma com e sem a adição de 0,70% de extrato hidroalcoólico de própolis, com 13 repetições e 2 aves por unidade experimental. Foram avaliados: taxa de postura, conversão alimentar, peso absoluto e relativo de gema, albúmen e casca, altura e largura do ovo, gravidade específica, coloração da gema, espessura de casca, unidade Haugh, índice de albúmen e de gema. O extrato hidroalcoólico (0,70%) proporcionou aumento da taxa de postura e melhora da conversão alimentar, ao nível de proteína de 17,70%, além de promover coloração mais acentuada da gema. Portanto, o nível de 0,70% de extrato hidroalcoólico de própolis pode ser usado em rações para poedeiras Isa Label, já que melhora características desejáveis como taxa de postura, conversão alimentar e coloração da gema, quando as rações têm nível de proteína de 17,7%. E o nível de proteína de 16,82% pode ser usado para poedeiras Isa Label, sem prejuízo no desempenho

Objectives of thesis: 1.To evaluate the effect of pH and total hardness of water on the development of eggs in aquarium fish Corydoras paleatus, Corydoras paleatus albin, Tanichthys albonubes, Tanichthys albonubes gold, Aphyosemion gardneri, Aphyosemion gardneri gold. 2.Recommendation of optimal values of total hardness of water and pH for successful development of eggs for each monitored aquarium fish. Based on published data in available literature sources and on own experiments, it was evaluated how total water hardness (CM) and pH influences development of aquarium fish eggs of Aphyosemion gardneri, Aphyosemion gardneri gold, Tanichthys albonubes, Tanichthys albonubes gold, Corydoras paleatus, Corydoras paleatus albin.

Submitted by Rosangela Silva (rosangela.silva3@unioeste.br) on 2018-06-25T14:39:55Z No. of bitstreams: 2 Douglas Ticiani Final.pdf: 15543424 bytes, checksum: 41d19b956e10c7116d923cf4330efe56 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2018-06-25T14:39:55Z (GMT). No. of bitstreams: 2 Douglas Ticiani Final.pdf: 15543424 bytes, checksum: 41d19b956e10c7116d923cf4330efe56 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-06-11 / Run of River hydroelectric systems, beyond fragments aquatic environments, drastically reduced as flow discharges in the Reduced Flow Stretch (RDS). The objective of this study was to evaluate the influence of TVR formation and environmental variables on composition and spatial distribution of eggs and larvae, in dams cascade operating with free threshold and under the Run of River system. Fortnightly nocturnal samplings were carried out between October/2016 and January/2017, in four points of influence area of the Rio das Antas Energy Complex, Patos Lagoon ecoregion. The ichthyoplankton was collected with conical-cylindrical networks, in three sections of the river and by a light trap per point. The eggs were quantified, while larvae were identified using the sequence regression development technique, framed in larval periods (larval yolk, pre-flexion, flexion and post-flexion) and juvenile. Were collected 5,681 eggs, 2,124 larvae and 43 juveniles, belonging to 25 taxonomic groups. Small species, sedentary and without parental care (small characids) had the highest total abundance and density. Significant spatial differences were identified in egg and larval densities, as well in the stages of ontogenic development, influenced by flow, temperature, pH and dam-free river stretch. The higher density of upstream larvae and the greater species richness downstream of the complex, indicate disruption in the natural processes of drift and the displacement of many species in the upstream direction. Higher flow rates positively influenced the use of RDS as spawning sites. / Hidrelétricas que desviam o rio, além de fragmentar os ambientes aquáticos, reduzem drasticamente as descargas de fluxo hídrico nos Trechos de Vazão Reduzida (TVRs). O objetivo desse estudo foi avaliar a influência da formação de TVR’s e das variáveis ambientais na composição e a distribuição espacial de ovos e larvas, em um complexo de barragens em sequência, que operam com soleira livre e sistema de desvio do rio e soleira livre. Foram realizadas amostragens quinzenais noturnas entre outubro/2016 e janeiro/2017, em quatro pontos na área de influência do Complexo Energético Rio das Antas, ecorregião Lagoa dos Patos. O ictioplâncton foi coletado com redes cônico-cilindricas, em três secções do rio e por uma armadilha luminosa por ponto. Os ovos foram quantificados, enquanto as larvas foram identificadas através da técnica de sequência regressiva de desenvolvimento, enquadradas em períodos larval (larval vitelino, pré-flexão, flexão e pós-flexão) e juvenil. Foram coletados 5.681 ovos, 2.124 larvas e 43 juvenis, pertencentes a 25 grupos taxonômicos. Espécies de pequeno porte, sedentárias e sem cuidado parental (pequenos caracídeos) foram as maiores contribuintes para a abundância e densidade totais. Foram identificadas significativas diferenças espaciais nas densidades de ovos e larvas, bem como nos estágios de desenvolvimento ontogênico, influenciadas pela vazão, temperatura, transparência, pH e trecho de rio livre. A maior densidade de larvas à montante e a maior riqueza de espécies a jusante do complexo, indicam interrupção nos processos naturais de deriva e dispersão de muitas espécies no sentido montante. Maiores vazões influenciaram positivamente a utilização dos TVRs como sítios de desova.

Per- and polyfluoroalkyl substances (PFASs) are a group of toxic and persistent organic compounds. Their properties make them extremely resistant and they have been shown to have bioaccumulation and toxic properties in the environment and also to biomagnify in both aquatic and terrestrial food webs. This study has analysed different PFASs in African Darter (Anhinga rufa) eggs from five sites along Vaal River; Orange River, South Africa. Sixteen of 23 analysed PFASs were detected and quantified, and the homologue profiles were studied from all five sites. Total perfluorooctane sulfonic acid (PFOS) (all structural isomers) was the predominated compound of all PFASs, accounting for 88-98% for all sites, with a median concentration range of 58 ng/g ww to 2473 ng/g ww. The second highest concentration was found for perfluorodecanoic acid (PFDA) (1.9-42 ng/g ww), followed by perfluorononanoic acid (PFNA) (1.1-14 ng/g ww) and perfluorohexane sulfonate (PFHxS) (0.68-6.0 ng/g ww). The results showed significantly that the three up-stream sites (Welverdiend, Schoemansdrift and Orkney East) had similar patterns and that eggs from Schoemansdrift had the highest levels of PFASs. This may indicate the same source of origin for these three sites and that Schoemansdrift are closest to the contamination source. The three sites (Welverdiend, Schoemansdrift and Orkney East) with similar pattern is closest to Gauteng, which can be where the emission source is located, because it is an industrial area. Perfluoroalkyl carboxylic acids (PFCAs) and PFOS might originate from different sources and the source for PFCAs could be degradation of fluorotelomer-based precursors. Structural isomer profiles of PFOS showed similar results as the PFAS homologue patterns, which give further indication of the source of origin. The contribution of linear PFOS (L-PFOS) to the total amount of PFOS was between the range of 94 and 97%. Bloemhof had the highest concentration of branched isomers among all sites. The L-PFOS concentrations in Bloemhof were also significantly differ from Schoemansdrift. This indicate two different sources between Bloemhof and the three up-steam sites, or an effect of environmental fractionation.

Reproduction in vertebrates, including fishes, is under control of the brain-pituitary-gonad (BPG) axis. The female three-spined stickleback, Gasterosteus aculeatus, produces egg clutches at intervals of a few days and spawns them in a nest built by male. Following ovulation, eggs are stored in the ovarian cavity surrounded by the ovarian fluid (OF). If spawning or spontaneous release do not occur, the eggs can undergo overripening, a phenomenon occurring both in nature and captivity. In this PhD thesis, the changes of reproductive hormones and vitellogenesis were studied at overripening of eggs and over the natural spawning cycle. OF properties were also examined at overripening of eggs and after treatment with sex steroids. Plasma levels of steroids: testosterone (T), estradiol (E2), 17,20β-dihydroxypregn-4-en-3-one (17,20β-P) and 17,20β,21-trihydroxypregn-4-en-3-one (17,20β,21-P) were measured by radioimmunoassay, and relative mRNA levels of the pituitary gonadotropins (fsh-β/lh-β), brain gonadotropin-releasing hormones (gnrh2/gnrh3) and kisspeptin and its receptor (kiss2/gpr54) by qPCR. Overripening of eggs was accompanied with a significant reduction in most of endocrine parameters of BPG axis (T, E2, 17,20β-P; lh-β; kiss2, gpr54). Low level of hormones could be advantageous for the overripe egg-bound females, since this would reduce further ovulations giving higher chances to survive and reproduce again. Over the 3-day spawning cycle, T and E2 were highly correlated, showed cyclicity with low levels at ovulation and increasing from 24 and 6 hours post-spawning (hps), respectively. Spawning may give rise to this increase as these rises did not occur if release of the eggs does not happen (overripe females). A peak at pituitary lh-β mRNA levels appeared 48 hps, a day before the next ovulation. No significant changes were found for the other studied hormones. Vitellogenesis was studied by measurement of the vitellogenin mRNA levels in the liver by qPCR. The levels were highest at 24 and 48 hps and were positively correlated to both E2 and T over the cycle. However, changes were small suggesting a rather continuous vitellogenesis over the stickleback spawning cycle which could be an advantage for a multiple spawner with a limited spawning season. Overripening reduced vitellogenin mRNA levels but did not abolish it. OF amount was diminished in overripe females and had a lower viscocity but higher dry weight and protein levels than in non-overripe ovulated females, suggesting that changes in OF properties are related to the egg overripening. The effects of steroids were studied using Silastic capsules. T and 17,20β-P induced an increase of OF amount, but protein levels were only increased in 17,20β-P-treated females, proposing a role of this steroid in the control of OF secretion. 1-D SDS-PAGE showed that OF contained several proteins, some of them came from eggs, but no consistent differences between groups. Concluding, the knowledge of the reproductive physiological changes is important for understanding their essential roles in the production of viable eggs in this species but also in the reproductive physiology of female fishes in general. / <p>At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 3: Manuscript. Paper 4: Manuscript.</p>

M.Sc. (Zoology) / Pans are endorheic wetlands, and are abundant in South Africa in a band from the western Free State into Mpumalanga. The pan environment experiences daily and seasonal fluctuations in physico-chemical conditions. The physico-chemical variables are influenced by the local climatological and hydrological conditions, and are all inter-related. An imbalance of one variable can have countless effects on the others. The physico-chemical composition of the water ultimately determines the existence of the biota in such wetlands. Branchiopod crustaceans are a unique group of fauna which have various morphological, physiological and behavioural adaptations which enable them to survive in these variable environments. One such adaptation is the production of dormant egg banks. These eggs reside within the sediment through the dry phase and hatch during a following wet phase when conditions are favourable. Due to the endorheic nature of pans they are more vulnerable to anthropogenic stress. Anthropogenic activities are having profound effects on the integrity of these ecosystems. Agricultural and mining activities have some of the largest influences. The impacts that the following activities have include: the over utilisation of water, decreased periods of inundation, erosion and sedimentation, effluent discharge and direct habitat destruction. Many wetlands as a result are experiencing a rapid loss in biodiversity. Mining activities are on the increase especially in the Highveld region of southern Africa. Many of these wetlands are already (and will be in the future) affected by mining activities, making the effect of acid mine drainage (AMD) on the biota a priority concern. In conjunction with the uniqueness and vulnerability of pan ecosystems it is necessary to find new ways of monitoring such environmental impacts in the shortest time possible with minimal efforts, for the benefit of both the environment and researchers involved. This study therefore aimed to assess the diversity of branchiopod crustaceans hatching from egg banks of selected pans and obtain a reference community structure. It also aimed to assess the impacts AMD could have on the hatching success of branchiopods from these egg banks with the objective to determine whether these egg banks are still viable after exposure. To achieve the stated aims and objectives, sediment samples were collected from selected pans in mining regions of the country. Regions selected included Chrissiesmeer in the Mpumalanga province, Wesselsbron in the Free State province and Delareyville in the North West province. The sediment was used for hatching experiments in the laboratory. Pan sediment was exposed to three different treatments which included two salt solutions (1000 mg/l and 1500 mg/l respectively) and AMD. The salt solutions served as controls while the AMD served as an exposure. The number of nauplii hatching was counted in the controls and compared to the number of nauplii hatching in the AMD. The diversity of nauplii was also assessed and compared between controls and the AMD. The recovery potential of eggs exposed to AMD was also assessed by exposing the sediment treated with AMD to distilled water after its removal to get a better understanding on the effects of AMD at the community level. Results from the control treatments indicated that most pans have a range of taxa hatching that follow patterns of pan succession. Between the 1000 mg/l and 1500 mg/l controls there was no treatment that proved superior to the other. Representatives of all four orders of branchiopoda hatched from the experiments. The North West and Free State pans were the most diverse and had had the greatest abundances of individuals hatching. Spatially all three provinces differed in the diversity of individuals hatching from pans, as there were distinct differences in the taxonomic compositions. Although taxonomic composition of pans grouped together per province, pans from just a single province were largely dissimilar. Results from the AMD treatments indicated that AMD has a negative effect on the hatching ability of branchiopod crustacean eggs. Eggs that were initially exposed to AMD were unable to hatch in its presence. The recovery experiments indicated that recovery after exposure is limited as recovery only occurred in a few pans. The taxonomic composition of nauplii in the pans where recovery took place was altered and less diverse than the taxonomic composition found in the respective control treatments. Overall it was found that hatching experiments can be used as a monitoring tool in lieu of field sampling. Hatching experiments showed that AMD is detrimental to the branchiopod egg banks, inhibiting the ability of eggs to hatch in its presence. Recovery can take place but the recovery potential is low. Since the recovery potential of the egg banks is low, pans which are affected by AMD could experience extinction of the entire branchiopod community in years to come. As branchiopod communities are unique among pans, and serve as an important food source for many aquatic bird species, their extinction will bring about further losses in biodiversity.

Text in English / The study was conducted in two different types of natural ventilated housing systems; one featured with conventional laying cages while other had free-range system features. The aim was to analyse productivity and economic benefit or loss of the Lohmann Brown Lite on different housing (Free-range system and Conventional cage system) with an evaluation of production cost. The total of 49 700 point of lay Lohmann Brown Lite layers pullets were placed in four natural ventilation free-range houses each consist of three rows of two tier conventional laying cages. The other 40 000 point of lay Lohmann Brown Lite layers pullets were place in four free-range houses each with placement of 10 000 chickens. The Cobb Douglas production model was used to determine the productivity of two different housing system by factoring the fixed cost and variable cost of the entire production process. The breakeven point tool was used to analyse the point where the total revenue equals the total variable and fixed expenses and the cost volume profit by measuring the profitability of each housing system (Nabil et al. 2014). The results of the study revealed different productivity between conventional cage system (82.94%) and the free-range system (77.46%). These results led to the acceptance of hypothesis 1 that “the production of Lohmann Brown Lite is the same when they are kept on the free-range system or conventional cage system. The capital investment and operation on conventional cage system showed breakeven at 43 months while free-range system showed breakeven at 60 months. The economic benefit analysis shows that the free-range system has cost benefit of R0.29 and conventional cage system has a cost benefit of R0.26. It means for every rand spent, farmer may get R0.29 Rand as profit for free-range system and every rand spent for conventional cage system can get R0.26 Rand. The economic benefit to farmer is greater on free-range system that lead to acceptance of hypothesis 2 that the economic benefit of egg production is greater on the free-range system compared to conventional cage system. Free-range housing system remain the best alternative to replace the conventional cage housing system. / Agriculture and  Animal Health / M.Sc. (Agriculture)