The present thesis concerns the molecular imaging of opioid receptors and human butyrylcholinesterase with the aid of tailored probes, which are suitable for the respective applied imaging techniques. The first part focusses on imaging of opioid receptors with selective probes using total internal reflection- and single molecule fluorescence microscopy. Design and synthesis of the ligands are presented and their pharmacological characterization and application in microscopy experiments are shown. The second part of this thesis focused on the development of 18F-labeled, selective radiotracers for imaging of butyrylcholinesterase via positron emission tomography. The design and synthesis of each a reversible and pseudoirreversible 18F-labeled tracer are presented. After evaluation of the binding properties of each tracer, their initial application in ex vivo autoradiography- and preliminary in vivo microPET studies is described and analyzed. / Die vorliegende Arbeit beschäftigt sich mit der molekularen Bildgebung von Opioidrezeptoren und der humanen Butyrylcholinesterase mithilfe von maßgeschneiderten Verbindungen, die jeweils optimal geeignet für die angewendeten Bildgebungstechniken sind. Der erste Teil behandelt die Bildgebung von Opioidrezeptoren durch selektive Liganden mittels interner Totalreflexionsfluoreszenzmikroskopie- und Einzelmolekül-Mikroskopie. Design und Synthese der Liganden werden beschrieben und ihre pharmakologische Charakterisierung und Anwendung in Mikroskopieexperimenten werden gezeigt. Der zweite Teil der Arbeit beschäftigt sich mit der Entwicklung von 18F-markierten, selektiven Radiotracern für die Bildgebung der Butyrylcholinesterase mittels Positronen-Emissions-Tomographie. Das Design und die Synthese jeweils eines reversiblen und pseudo-irreversiblen, 18F-markierten Tracers werden beschrieben. Nach der Bewertung der Bindungseigenschaften beider Tracer am Enzym, wird ihre erste Anwendung in ex vivo Autoradiographie- und vorläufigen in vivo microPET Studien beschrieben und ausgewertet.

Die Detektion des Prostataspezifischen Membranantigens (PSMA) mittels kombinierter Positronenemissions- und Computertomographie (PET/CT) ist ein etabliertes diagnostisches Verfahren bei Patienten mit Prostatakarzinom. Hierbei ist bislang unklar, ob und wie eine bereits eingeleitete Androgendeprivationstherapie (ADT) die diagnostische Genauigkeit der PSMA-PET/CT beeinflusst. Ziel dieser Arbeit war es, die Detektionsrate der PSMA-PET/CT mit 68Ga-PSMA I&T unter ADT in Abhängigkeit des PSA-Wertes zu evaluieren und mit einer Kontrollgruppe ohne ADT zu vergleichen. In dieser retrospektiven Studie wurden Daten von Patienten mit biochemischem Rezidiv nach radikaler Prostatektomie analysiert, welche zwischen 2014 und 2018 eine PSMA-PET/CT am Universitätsklinikum Würzburg erhalten haben. Mittels Propensity Score Matching wurde für die Patienten mit ADT innerhalb der letzten 6 Monate vor Durchführung der PSMA-PET/CT eine Kontrollgruppe ohne ADT erstellt. Die Patienten mit ADT (n=62) wiesen eine signifikant höhere Detektionsrate auf als die Patienten ohne ADT (n=62). Die Traceranreicherung unterschied sich nicht signifikant in beiden Gruppen. Dagegen wiesen die Patienten mit ADT jedoch eine signifikant höhere Tumorlast auf und hatten häufiger Knochen- und Organmetastasen, sodass als Ursache für die höhere Detektionsrate der PSMA-PET/CT bei Patienten mit ADT ein fortgeschritteneres Tumorstadium angenommen wurde. Die Detektionsrate war bei den Patienten mit ADT auch bei niedrigen PSA-Werten hoch. Es scheint daher nicht erforderlich zu sein, eine bestehende ADT vor Durchführung der PSMA-PET/CT im biochemischen Rezidiv abzusetzen und damit das Risiko einer Krankheitsprogression einzugehen. Die Korrelation des PSA-Wertes mit der Tumorlast in der PSMA-PET/CT war bei Patienten mit ADT geringer ausgeprägt als bei Patienten ohne ADT. Patienten unter ADT könnten daher von einer regelmäßigen Durchführung der PSMA-PET/CT zur Überwachung der Krankheitsprogression profitieren. Hier bleibt allerdings eine Kosten-Nutzen-Analyse abzuwarten, da dies deutlich aufwendiger und teurer ist als die Bestimmung des PSA-Wertes. / Detection of prostate specific membrane antigen (PSMA) by combined positron emission tomography and computed tomography (PET/CT) is an established diagnostic procedure in patients with prostate cancer. So far it is unclear whether an already initiated androgen deprivation therapy (ADT) influences the diagnostic accuracy of PSMA-PET/CT. The aim of this study was to evaluate the effect of ADT on the detection rate of PSMA-PET/CT with 68Ga-PSMA I&T. In this retrospective study, data from patients with biochemical recurrence after radical prostatectomy who received PSMA-PET/CT at the University Hospital of Würzburg between 2014 and 2018 were analyzed. Propensity score matching was used to create two balanced groups of 62 patients who either did or did not receive ADT within six months before imaging. The detection rate of PSMA-PET/CT was significantly higher in the group of patients with ADT than in the control group without ADT. Tracer accumulation was not significantly different in both groups. In contrast, patients with ADT had significantly higher tumor burden and more frequent bone and organ metastases, suggesting a more advanced disease stage as the reason for the higher detection rate of PSMA-PET/CT in patients with ADT. The detection rate was high in patients with ADT even at low PSA levels. Therefore, the withdrawal of ADT before PSMA-PET/CT in biochemical recurrence, thereby risking disease progression, cannot be recommended. The correlation of PSA level with tumor burden in PSMA-PET/CT is lower in patients with ADT than in patients without ADT. Patients with ADT may therefore benefit from routine performance of PSMA-PET/CT to monitor disease progression. However, a cost-benefit analysis remains to be performed, as this is significantly more time-consuming and expensive than the measuring of the PSA value.

Orange County, FL has been experiencing ozone concentrations in the past several years which in some cases exceeded the national and state standards. The high concentration of ground level ozone can cause a variety of health problems including chest pain, coughing, throat irritation, and congestion or it can worsen bronchitis, emphysema, and asthma. Other effects include reduction of agricultural crop and commercial forest yields, lower growth and survivability of tree seedlings, and higher susceptibility of plant to diseases, pests and other stresses such as harsh weather. The ozone generation rate is directly related to the ambient concentration of nitrogen oxides (NOx) and volatile organic carbons (VOCs). These two air pollutants, mostly produced from combustion of fossil fuels, react with oxygen to form ozone in presence of sunlight. In urban areas, ozone generation rate can be decreased by reduction of ozone precursors, NOx and VOCs. The Air Quality Research group of University of Central Florida proposed that one of the emission reduction strategies be for school bus fleets in the area. School buses were chosen because of their important impact on ambient air quality in general and on student health in particular. There were about 473,000 school buses in the 2004-05 school year nationwide which traveled for a total mileage of about 4 billion miles in that year. Orange County Public School (OCPS) system owns about 1400 school buses which traveled about 17 million miles in 2005-06 school year, serving 71000 students. The use of diesel fuels, Ultra Low Sulfur Diesel (ULSD, diesel fuel containing 15ppm sulfur) and Biodiesel (B20, a mixture of 20% biodiesel and 80% ULSD), were chosen as the first proposed action to be studied. Also the effects of transportation parameters, average speed and idling time on fleet emissions were selected to be reviewed. This report reviews the fuel option and transportation parameters, effects on school bus fleet emissions and it does a comparison analysis in order to show advantages and disadvantages of each fuel. The Conventional Diesel (CD) and ULSD emissions were estimated by using MOBILE6.2 model, and effects of B20 on emissions were derived from published studies. It was found that using B20 or ULSD can reduce the emissions significantly for the most of major pollutants but in the case of NOx, the percentage changes is not certain yet and more investigation is required. Emissions vary for different average speeds and 27 miles per hour can be defined as the optimum average speed. Also reduction of idling time is an excellent control option for decreasing emissions, and should be considered for OCPS.

Hydrogen sulfide (H2S), a major odorous component emitted from construction and demolition debris landfills, has received increasing attention. Besides its unpleasant odor, long-term exposure to a very low concentration of H2S can cause a public health issue. Although cover materials such as soil and compost are recommended to be used routinely to control an odor problem from the landfills, the problem still remains. Autotrophic denitrification may have environmental applications including treatment of water, groundwater, wastewater or gaseous streams contaminated with sulfur and/or nitrogen compounds. However, there have been no studies reported in the literature on H2S removal using autotrophic denitrification from landfills. This study, therefore, investigated the application of autotrophic denitrification incorporated into landfill covers in order to evaluate the feasibility of controlling H2S emissions generated from landfills. Research was investigated by two techniques, microcosm and laboratory-scale column studies. The microcosm experiments were conducted to evaluate the kinetics of autotrophic denitrification in various cover materials with H2S-nitrate as electron donor-acceptor couple. Cover materials including soil, compost and sand were tested and nitrate was added. Based on the microcosm study results, the addition of nitrate into soil and compost can stimulate indigenous autotrophic denitrifying bacteria which are capable of H2S oxidation biologically under anoxic conditions. Results also demonstrated that some amount of H2S can be removed physically and chemically by soil or compost. There was no H2S removal observed in sand microcosms. Rapid H2S oxidation to sulfate was achieved, especially in soil. Zero-order kinetics described the H2S oxidation rate in soil and compost microcosms. The rates of sulfide oxidation under autotrophic denitrification in soil and compost were 2.57 mg H2S/d-g dry soil and 0.17 mg H2S/d-g dry compost, respectively. To further explore H2S removal in a landfill biocover, two sets of column experiments were run. The first set of columns contained seven cm of soil. The autotrophic column was prepared with 1.94 mg KNO3/g dry soil; an identical control column was prepared without nitrate. A gas stream was introduced to the columns with a H2S concentration of 930 ppm. The second set contained seven cm of soil, with both an autotrophic (0.499 mg KNO3/g dry soil) and a control column. Influent H2S concentration was 140 ppm for the second set. Column studies supported the results of microcosm studies; removal of H2S was observed in all columns due to the capacity for soil to absorb H2S, however autotrophic columns removed significantly more. The higher concentration of H2S resulted in partial oxidation to elemental sulfur, while sulfate was found at levels predicted by stoichiometric relationships at the lower concentration. H2S oxidation in the column with higher loading was found to follow zero-order kinetics. The rate of H2S oxidation was 0.46 mg H2S removed/d-g dry soil. Economic comparison of cover systems including autotrophic denitrification, soil amended with lime, fine concrete, and compost covers were analyzed. Based on a case-study landfill area of 0.04 km2, the estimated H2S emissions of 80,900 kg over the 15-year period and costs of active cover system components (ammonium nitrate fertilizer, lime, concrete and compost), autotrophic denitrification cover was determined to be the most cost-effective method for controlling H2S emissions from landfills.

Background. Using JavaScript frameworks has become a popular approach for developing web applications. However, the large bundle sizes associated with framework usage may not always be necessary from a sustainability standpoint. Utilizing a framework or not could potentially impact a web application’s performance and carbon emissions.  Objectives. This study aims to identify sustainable approaches to frontend web development and investigate whether a comparable difference in performance, energy consumption, and carbon emissions exists between applications built using a framework like React.js versus those developed using vanilla JavaScript.  Methods. A literature review was conducted to identify important factors for creating sustainable web applications from previously published materials. An empirical study was performed where two test applications were created; one developed in vanilla JavaScript and one using the React.js framework. Experiments on these prototypes compared performance and estimated carbon emissions on the applied technologies.  Results. When researching literature published on sustainability within web development, the factor most frequently mentioned was web performance optimization. The results of the empirical study found that the application written in vanilla JavaScript achieved higher performance metrics and lower estimated carbon emissions than the prototype developed with React.js.  Conclusions. This thesis has found that web performance optimization is an important, powerful, and easy-to-use approach for achieving more sustainable web applications. This study has found several important factors for creating sustainable web applications, which can significantly impact an application’s carbon emissions. The study found that the best-performing test application achieved the lowest estimated carbon footprint. This thesis recommends that developers make informed decisions and consider sustainability when choosing a development strategy, like using a framework or not for future projects.

Emissions related to aviation have during the last decades become an important topic of discussion. Besides carbon dioxide ($CO_2$) which is the major pollutant from air travel, other gas emissions such as hydrocarbons ($HC$), nitrogen oxides ($NO_x$), carbon monoxide ($CO$) and sulfur oxides ($SO_x$) also need to be investigated. The work within this field has traditionally been challenged by the fact that aircraft emission calculations often required engine proprietary data which usually is difficult to obtain. However, in recent years other methods have been developed and this report investigates one such method, namely The Boeing Fuel Flow Method 2. The analysis is carried out on an ATR 72-500 turboprop aircraft flying at $13000$ feet from Visby to Bromma, Sweden. The method uses continuous data of fuel flow and altitude together with estimated emission index data at sea level for the specific engine to estimate the amount of emissions emitted during the flight. From this it was possible to determine the levels of $HC$,$NO_x$,$CO$, $CO_2$ and $SO_x$ emitted during the different stages of flight. There was a clear trend that $HC$ and $CO$ emissions were the highest at low fuel flow levels, i.e. at low power, while $NO_x$ increased with increasing fuel flow. Emission levels of $CO_2$ and $SO_x$ were found to be proportional to fuel flow. In addition, two alternative trajectories at $10000$ and $24000$ feet were studied. When comparing the $10000$ feet route with the original $13000$ feet route the the level of $NO_x$, $CO_2$ and $SO_x$ were unaffected while $HC$ and $CO$ decreased as the period of high fuel flow were shortened. In the $24000$ feet route the levels of $HC$, $CO$ and $SO_x$ were unaffected while the level of $CO_2$ and $NO_x$ decreased. This decrease can be explained by the lowered fuel flow rate as air resistance is significantly lower at $24000$ feet compared to $13000$ feet.

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