Many dogs suffer from separation anxiety and become excessively stressed when their owner is absent. Since many dogs are left home alone daily, their welfare is at stake. Attention bias tests (ABTs) have previously been used as a way to measure welfare in other non-human animals. The aim of this study was to investigate attention bias in dogs with separation anxiety and dogs without separation anxiety in situations of different short-term emotional states. Three ABTs were performed to assess this, the first one was baseline and included no treatment (neutral) prior to the ABT. The second one was play which had a play treatment (positive) before the ABT, where the dog played with their owner for 2.5 min. The third one was isolation which had an isolation treatment (negative) prior to the ABT, where the dog was alone for 2.5 min. The owner was only present during the ABT in baseline and play. The results showed that separation anxiety only had an effect on barking during isolation, where dogs with separation anxiety barked more. Dogs performed more behaviours related to the owner being absent during isolation: more vocalisation, more looking at the door, being closer to the door (zone five), and being less in the area where the owner stood during baseline and play (zone six), compared to both baseline and play. To my knowledge, the usage of ABTs to measure welfare in dogs are few, and this study revealed that separation anxiety does not affect attention bias.

Bird song can teach us much about animal behaviour and evolution. This study presents a type of song variation that has yet to be extensively studied. The Monotonous Lark Mirafra passerina is a nomadic lark with an iconic, simple and repetitive song. It has been noted to have a large song variation between irruptions, but limited variation in the same irruption, a behaviour previously, to my knowledge, unknown in the avian world. This study aims to describe the song and analyse the variation within and between irruptions, and try to explain the underlying mechanisms behind the variation. This article studies the song by analysing song material spanning 30 years with 140 samples, most from South Africa. The whole song and the syllables were measured. A custom edit distance and Euclidean distance were used to quantify song differences. Principal component analysis was performed on both syllable measurements and the whole song. The results showed a larger song variation between irruptions compared to within irruptions. It also showed that the song varies greatly between irruptions; however, a general pattern of how the song is structured was found. In addition, some indications of song retention between years were noticed. This study describes a unique example of avian behaviour that can broaden our knowledge of animal communication and its evolution and development.

We can describe cognition as the mental processes involved when processing signals and information from our surroundings. Despite being vital for our actions, these processes can be biased by emotions, which results in a judgement bias of ambiguous information. Depressed individuals tend to be pessimistic about such ambiguous information, while individuals under normal or good condition, tend to be optimistic. This is true also for animals. Based on this, cognitive judgement bias tests are developed to measure the affective state of individuals. However, cognitive judgement bias tests require extensive pre-test training for animals to learn positive and negative reference cues. An alternative to using responses to pre-learnt cues could be to use naturally aversive stimuli instead. Eyespot patterns on lepidopterans can be aversive to birds. However, it is scarcely investigated if eyespot patterns can be used to measure affective state. The aim of my study was therefore to investigate if eyespots patterns can replace classic cues in cognitive judgment bias tests measuring affective state. I did so by comparing behavioural responses of red junglefowl chicks (Gallus gallus) to both eyespot patterns and classical cues in a cognitive judgement bias test. Responses correlated between some cues in the two tests, suggesting that eyespot patterns may work as a replacement of pre-learnt cues. However, no differences in responses to the eyespot patterns was found, and so further work is needed to improve the design of eyespot cues to obtain a clearer correlation between responses to eyespot patterns and classical pre-learnt cues in cognitive judgement bias tests. As less training is needed, such improved tests could have positive implications, and be a simpler and more user-friendly way to measure affective state in animals.

The Red Junglefowl is a species of bird that was domesticated by humans around 8 000 years ago. Over time, domestication has led to changes in behavior and morphology, made possible by reduced fear of humans. Fear has been shown to affect cognitive processes, such as the way ambiguous stimuli are perceived by the individual. In this study, I observed the behavior of Red Junglefowl hens that had been selected for either high or low fear of humans to see how the early stages of domestication would affect their tendency to make pessimistic or optimistic judgements. The hens were put in a test arena where they were exposed to positive, negative, and ambiguous stimulus cues, and the time taken to approach each was measured. Hens that had been selected for low fear of humans had overall, albeit not significantly, shorter latencies to approach ambiguous cues. In other words, they were more likely to make optimistic judgements. The results were also affected by the order each stimulus cue was presented. I conclude that domestication may influence the way Red Junglefowl perceive ambiguous stimuli by reducing pessimism.

This literature study is about rehoming of dogs, the focus is on investigating the reason why dog get rehomed, what behavioral problems that can occur when the dog is rehomed and how to work with these problems. The study concluded that the main reasons for a dog to be rehomed are behavioral problems in the dog, health problems concerning the owners, changed living conditions and lack of time. More reasons can also contribute, and these are also discussed. Additional behavioral problems that can occur when the dog is left in a shelter include separation anxiety and stress-related behavioral problems. This study describes the stress reaction and how the stress reaction looks and work and how the stress hormone cortisol can be used measuring stress levels in the short and long term.  For the last part of the study, the study concluded how a person can work with the dog´s behavioural problems. According to research, positive reinforcement is the best method to use when training dogs. This type of trainings method strengthens the relationship between the dog and the owner and thus reduces the risk of a dog being rehomed. The study also concluded that training methods that use aversive methods can make the dog stressed, make the dog feel anxious and lead to that the dog start expressing aggressive behaviour.

During the course of time, artificial selection has given rise to a great diversity among today's dogs. Humans and dogs have evolved side by side and dogs have come to understand human body language relatively well. This study investigates whether selection pressure and domestication could reveal differences in dogs’ skill to interpret human directional cues, such as distal pointing. In this study, 46 pet dogs were tested from 27 breeds and 6 crossbreeds for performance in the two-way object choice task. Breeds that are selected to work with eye contact of humans were compared with breeds that are selected to work more independently. Dogs of different skull shape were also compared, as well as age, sex and previous training on similar tasks. No significant differences in performance were found between dogs of various age, sex or skull shape. There was a tendency for significant difference in performance if the dog had been previously trained on similar tasks. When dogs that made 100% one-sided choices were excluded, a tendency appeared for there to be a difference between the cooperative worker breeds compared to the other breeds for the time it took for dogs to make a choice. There is a correlation between the number of correct choices made and the latency for the dogs from being release to making a choice (choice latency). All groups of dogs, regardless of my categorization, performed above chance level, showing that dogs have a general ability to follow, and understand, human distal pointing.

The dear enemy effect is a widespread behavioral phenomenon that promotes selective pacifism in order to minimize costs of territorial defense. The dear enemy hypothesis predicts heightened aggression towards unfamiliar conspecifics compared to familiar neighbors where territorial boundaries are established. The purpose of this study was to test the dear enemy hypothesis in Neolamprologus multifasciatus, a social cichlid fish endemic to the shell beds of Lake Tanganyika. They establish breeding groups that control distinct territories, each governed by a dominant male. Territories can be very densely spaced, and conspecific border intrusions generally trigger aggressive responses from the focal group. The dominant males are responsible for the majority of territory defense. In this field experiment, 27 breeding groups were each given two treatments, each consisting of artificial introduction of an out-of-group dominant male. One male originated from an immediately neighboring group, the other from a distant group.  A downward-facing camera recorded group members’ aggressivity exhibited towards the experimentally presented male. The behavior was manually scored according to a behavior ethogram and analyzed with either linear mixed effects models or generalized linear mixed effects models. No clear dear enemy effect was detected within Neolamprologus multifasciatus. Several reasons are proposed for this result. Firstly, a contingent “ceiling effect” in terms of aggressive behavior imaginably deterred efforts of dear enemy detection. Conversely, perhaps negligible necessity of stranger-neighbor recognition within Neolamprologus multifasciatus has resulted in discriminatory behavior being absent. In conclusion, no dear enemy effect was detected and future research will address possible alternative explanations.

Autism is a complex disorder with possible genetic, epigenetic and environmental components. As the etiology remains uncertain and an increase in incidence is suspected, the involvement of possible environmental risk factors has gained increasing attention. With this thesis, I aim to provide tools for assessing such risk factors. Firstly, I aim to construct a questionnaire for the analysis of an environmental component in the etiology of autism. Secondly, I aim to assess the importance of prenatal exposure to metals in certain diseases and thirdly I aim to construct a methodology enabling the analysis of the mitochondrial epigenome, which is especially interesting in relation to autism as mitochondrial diseases occur more frequently in an autistic population than in the general population. For the creation of the questionnaire the scientific literature was reviewed. The resulting questionnaire contains general, prenatal, neonatal and paternal risk factors. The metal analysis was conducted on the cord blood of patients who later developed autism, antinuclear antibodies positive rheumatoid arthritis or diabetes, which were then compared to healthy control subjects. My findings propose a link between elevated levels of cord blood cadmium or aluminum and rheumatic arthritis. In addition, elevated aluminum levels might be associated with autism. In regards to the analysis of the mitochondrial epigenome, to my knowledge, no standard protocol exists with frozen human whole blood as a source. In this thesis, I succeeded in creating the basis for such a protocol, however still needing several small modifications for an increased overall yield.

Worldwide, pharmaceutical compounds continue to increase in our aquatic environment. The predominant route into nature is through wastewater treatment plants since the elimination of residual pharmaceuticals is still not mainstream in WWTPs. Fluoxetine is an antidepressant which is commonly prescribed to treat human depression. Wastewater residual fluoxetine is typically found in waters around the world, and can thus affect exposed organisms, such as fish and invertebrates. However, how fluoxetine may affect mating behaviour in exposed organisms remains poorly understood, and particularly so in invertebrates. This is hampering our understanding of the consequences of our medicine leaking into nature because mating behaviour often affects fitness, and invertebrates are key organisms in food chains. Therefore, I here experimentally investigated long-term effects of environmental relevant concentration of fluoxetine (20 ng L-1) on mating behaviours of male and female freshwater isopod Asellus aquaticus. I demonstrate that fluoxetine reduced male mating attempts with receptive females. Further, there was a tendency for fluoxetine exposure to increase latency to form pre-copula. There was no effect of fluoxetine exposure on male latency to encounter females or female responses toward males. These results indicate that fluoxetine also can affect isopods by reducing mating behaviour. In the long-term, if reproduction is delayed or reduced, it may cause a reduction in populations and thus, alter the whole ecosystem.

<p>In this thesis, I experimentally investigate feeding selectivity in fallow deer (Dama dama), with respect to plant secondary compounds, especially tannins, which can decrease the quality of foods. I found that fallow deer avoided foods with higher amounts of tannic acid and Quebracho tannin, even though the deer ate some high-tannin food. The food choice was strongly dependent on the context in which the food was presented, so that the food choice in relation to tannin content was relative rather than absolute. When high-tannin food occurred at low frequency, the deer ate proportionally less from this type of food, at least when the difference in tannin content between the two foods was large. A basic implication is that an unpalatable plant type could benefit from its unpalatability, especially when occurring at low frequency. In experiments with two patches, the finding of a stronger within- than between-patch selectivity was mirrored in associational effects. First, low-tannin, palatable food was more eaten when occurring in a high-tannin patch, which corresponds to neighbour contrast susceptibility. Second, high-tannin, unpalatable food in a less defended patch was less eaten, which corresponds to neighbour contrast defence. A proximate cause of the associational effects can be the presence of a simultaneous negative contrast, which was experimentally demonstrated in an additional study. Individual differences in selectivity were present early in life and were consistent over five years, and selectivity was correlated with foraging exploratory behaviour. The results from this thesis suggest that fallow deer are selective in their food choice with respect to tannins from the beginning, and that the frequency of occurrence of different foods, but also the distance between foods and the complexity of presentation, influence the food choice. It is also suggested that a foraging behavioural syndrome is present in mammalian herbivores.</p>