Objetivo: Em anisométropes, comparar os valores médios individuais dos componentes oculares de ambos os olhos (poder da córnea, profundidade da câmara anterior, poder equivalente do cristalino e comprimento axial), correlacionar as diferenças dos componentes oculares com as diferenças de refração de ambos os olhos; verificar a contribuição total e a seqüência geral de influência das variáveis na diferença refrativa; e identificar o menor número de fatores que contenham o mesmo grau de informações expressas no conjunto de variáveis que influenciam na diferença refrativa. Métodos: Realizou-se um estudo transversal analítico em população de 77 anisométropes de duas ou mais dioptrias, atendida no ambulatório de Oftalmologia do Hospital Universitário da Faculdade de Medicina Nilton Lins, Manaus, Amazonas. Os anisométropes foram submetidos à refração estática objetiva e subjetiva, ceratometria e biometria ultra-sônica A-scan. A análise dos dados foi feita por meio dos seguintes modelos estatísticos: análise univariada, multivariada, de regressão múltipla e fatorial. Resultados: Não houve diferenças significativas na comparação dos valores médios individuais dos componentes oculares entre os olhos. Houve correlação negativa média entre a diferença refrativa e a diferença de comprimento axial (r=-0,64) (P<0,01) e correlação negativa fraca entre a diferença refrativa e a diferença de poder do cristalino (r=-0,34) (p<0,01). As variáveis analisadas responderam, no seu conjunto, por 78% da variação total para a diferença refrativa. A seqüência geral de influência das variáveis na diferença refrativa foi a seguinte: comprimento axial, poder do cristalino, poder da córnea e profundidade da câmara anterior. Foram identificados três fatores para a diferença refrativa: a) fator 1 (refração, comprimento axial); b) fator 2 (profundidade da câmera anterior, poder da córnea) e c) fator 3 (poder do cristalino). Conclusões: O estudo conduzido em 77 indivíduos com anisometropias variando de 2,00 a mais de 19,00 dioptrias, realizado para avaliar a influência dos componentes oculares, mostrou que o comprimento axial foi o principal fator causador das anisometropias, seguido pelo cristalino que contribuiu menos, depois pela córnea e profundidade da câmara anterior, com contribuições ainda menores. A investigação sugere falência no mecanismo adaptativo normal em anisometropia, o que poderia produzir não só descontrole do alongamento do comprimento axial (fator 1), mas também falência no controle do aplanamento da córnea e do aprofundamento da câmara anterior (fator 2) e no achatamento do cristalino (fator 3). / Purpose: To compare the individual means of ocular components of both eyes (corneal power, anterior chamber depth, crystalline lens power and axial length) in patients with anisometropia; to correlate the differences of the ocular components with refractive differences in both eyes; to verify total contribution and the sequence of influence that variables have in refractive differences, and to identify the smallest number of factors that contain the same level of information expressed in the set of variables that influence refractive difference. Methods: An analytical transversal study was carried out in 77 patients with anisometropia of two or more dioptres seen at the Ophthalmologic Clinic, University Hospital, Medical School Nilton Lins, Manaus, Amazon state. All participants were submitted to ophthalmologic exam which included objective and subjective cycloplegic refractometry, keratometry and ultrasonic biometry. Data analysis comprised the following statistical models: univariate, multivariate, multiple and factorial regression analyses. Results: There were no significant differences in the comparison of the individual means of the ocular components. There was negative correlation between refractive difference and difference of axial length (r=- 0.64; p<0.01) and weak negative correlation between refractive difference and crystalline lens power difference (r=-0.34; p< 0.01). The analyzed variables amounted to 78% of the total variation of refractive difference. The general sequence of variables influencing refractive difference was: axial length, crystalline lens power, cornea power, and anterior chamber depth. There were three factors identified for refractive differences: a) factor 1 (refraction, axial length); b) factor 2 (anterior chamber depth, cornea power), and c) factor 3 (crystalline lens power). Conclusions: Seventy-seven cases of anisometropia ranging from 2,00 to over 19,00 dioptres, examined for the individual components of refraction, showed that axial length was the major causative factor; crystalline lens have contributed less, followed by cornea and anterior chamber length. This study has suggested deficit of the normal adaptive mechanism in anisometropia that could produce not only axial elongation (factor 1), but also failure to control flattening of the cornea, deepening of the anterior chamber length (factor 2) and flattening of crystalline lens (factor 3).

Objetivo: Em anisométropes, comparar os valores médios individuais dos componentes oculares de ambos os olhos (poder da córnea, profundidade da câmara anterior, poder equivalente do cristalino e comprimento axial), correlacionar as diferenças dos componentes oculares com as diferenças de refração de ambos os olhos; verificar a contribuição total e a seqüência geral de influência das variáveis na diferença refrativa; e identificar o menor número de fatores que contenham o mesmo grau de informações expressas no conjunto de variáveis que influenciam na diferença refrativa. Métodos: Realizou-se um estudo transversal analítico em população de 77 anisométropes de duas ou mais dioptrias, atendida no ambulatório de Oftalmologia do Hospital Universitário da Faculdade de Medicina Nilton Lins, Manaus, Amazonas. Os anisométropes foram submetidos à refração estática objetiva e subjetiva, ceratometria e biometria ultra-sônica A-scan. A análise dos dados foi feita por meio dos seguintes modelos estatísticos: análise univariada, multivariada, de regressão múltipla e fatorial. Resultados: Não houve diferenças significativas na comparação dos valores médios individuais dos componentes oculares entre os olhos. Houve correlação negativa média entre a diferença refrativa e a diferença de comprimento axial (r=-0,64) (P<0,01) e correlação negativa fraca entre a diferença refrativa e a diferença de poder do cristalino (r=-0,34) (p<0,01). As variáveis analisadas responderam, no seu conjunto, por 78% da variação total para a diferença refrativa. A seqüência geral de influência das variáveis na diferença refrativa foi a seguinte: comprimento axial, poder do cristalino, poder da córnea e profundidade da câmara anterior. Foram identificados três fatores para a diferença refrativa: a) fator 1 (refração, comprimento axial); b) fator 2 (profundidade da câmera anterior, poder da córnea) e c) fator 3 (poder do cristalino). Conclusões: O estudo conduzido em 77 indivíduos com anisometropias variando de 2,00 a mais de 19,00 dioptrias, realizado para avaliar a influência dos componentes oculares, mostrou que o comprimento axial foi o principal fator causador das anisometropias, seguido pelo cristalino que contribuiu menos, depois pela córnea e profundidade da câmara anterior, com contribuições ainda menores. A investigação sugere falência no mecanismo adaptativo normal em anisometropia, o que poderia produzir não só descontrole do alongamento do comprimento axial (fator 1), mas também falência no controle do aplanamento da córnea e do aprofundamento da câmara anterior (fator 2) e no achatamento do cristalino (fator 3). / Purpose: To compare the individual means of ocular components of both eyes (corneal power, anterior chamber depth, crystalline lens power and axial length) in patients with anisometropia; to correlate the differences of the ocular components with refractive differences in both eyes; to verify total contribution and the sequence of influence that variables have in refractive differences, and to identify the smallest number of factors that contain the same level of information expressed in the set of variables that influence refractive difference. Methods: An analytical transversal study was carried out in 77 patients with anisometropia of two or more dioptres seen at the Ophthalmologic Clinic, University Hospital, Medical School Nilton Lins, Manaus, Amazon state. All participants were submitted to ophthalmologic exam which included objective and subjective cycloplegic refractometry, keratometry and ultrasonic biometry. Data analysis comprised the following statistical models: univariate, multivariate, multiple and factorial regression analyses. Results: There were no significant differences in the comparison of the individual means of the ocular components. There was negative correlation between refractive difference and difference of axial length (r=- 0.64; p<0.01) and weak negative correlation between refractive difference and crystalline lens power difference (r=-0.34; p< 0.01). The analyzed variables amounted to 78% of the total variation of refractive difference. The general sequence of variables influencing refractive difference was: axial length, crystalline lens power, cornea power, and anterior chamber depth. There were three factors identified for refractive differences: a) factor 1 (refraction, axial length); b) factor 2 (anterior chamber depth, cornea power), and c) factor 3 (crystalline lens power). Conclusions: Seventy-seven cases of anisometropia ranging from 2,00 to over 19,00 dioptres, examined for the individual components of refraction, showed that axial length was the major causative factor; crystalline lens have contributed less, followed by cornea and anterior chamber length. This study has suggested deficit of the normal adaptive mechanism in anisometropia that could produce not only axial elongation (factor 1), but also failure to control flattening of the cornea, deepening of the anterior chamber length (factor 2) and flattening of crystalline lens (factor 3).

Submitted by RODOLFO DE LIMA FACHINELLI null (r_fachinelli@hotmail.com) on 2018-04-04T18:54:51Z No. of bitstreams: 1 DISSERTAÇÃO - RODOLFO FACHINELLI.pdf: 1066915 bytes, checksum: 93987ac1254ae543ed84ff519a438005 (MD5) / Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2018-04-06T13:56:06Z (GMT) No. of bitstreams: 1 fachinelli_rl_me_bot.pdf: 1066915 bytes, checksum: 93987ac1254ae543ed84ff519a438005 (MD5) / Made available in DSpace on 2018-04-06T13:56:06Z (GMT). No. of bitstreams: 1 fachinelli_rl_me_bot.pdf: 1066915 bytes, checksum: 93987ac1254ae543ed84ff519a438005 (MD5) Previous issue date: 2018-02-22 / Objetivo: Comparar a ceratometria média aferida (KA) sob narcose em crianças submetidas à facectomia, por catarata congênita ou do desenvolvimento, com a ceratometria média obtida por cálculo teórico (KC), utilizando o poder dióptrico da lente intraocular (LIO) implantada e dados refracionais e biométricos pós-operatórios, a fim de evidenciar possíveis erros de aferição da KA devido a narcose. Métodos: Estudo retrospectivo realizado a partir da análise de dados coletados de prontuários de pacientes com catarata bilateral, congênita ou do desenvolvimento, que receberam tratamento cirúrgico no HC-FMB. Foram analisados 73 olhos de crianças que possuíam pelo menos um exame pós-operatório completo, cada momento em que determinado olho foi examinado foi considerado um elemento do conjunto amostral, totalizando 165 momentos. KC foi obtida a partir de fórmula teórica para cálculo de LIO, utilizando os dados biométricos pós-operatórios (AL e ACD), refratometria automatizada pós-operatória e poder dióptrico da LIO implantada. Foi calculada a diferença entre KA e KC (Desvio = KA – KC). Para análise estatística dos desvios encontrados e comparação entre KA e KC, foi realizado o teste de associação de Goodman e o teste não paramétrico de Kruskal-Wallis. Resultados: A média de idade no momento da cirurgia do primeiro olho foi 954,62 dias, com desvio padrão de ±794,14 dias, mediana de 953 dias, sendo a idade mínima de 44 dias e a máxima de 2659 dias. Vinte e três eram do sexo masculino (62,16%) e 14 eram do sexo feminino (37,84%). KA variou de 40,62D a 51,50D, com mediana de 45,25D, média de 45,32D e desvio padrão de ±2,37D. KC variou de 39,40D a 52,26D, com mediana de 44,49D, média de 44,54D e desvio padrão de ±2,41D. Os desvios (Desvio = KA – KC) variaram de -2,28D a 3,81D, com mediana de 0,83D, média de 0,79D e desvio padrão de ±1,18D. A relação entre KC e KA pode ser representada pela equação KA = 1,0172 Kc. Conclusão: A comparação entre KC e KA em crianças sob narcose evidenciou que há superestimação do valor aferido em relação ao calculado. A análise dos desvios encontrados mostrou tendência para maior superestimação quanto maior a KA com diferença significativa (p<0,05) nas aferições acima de 44,0D. / Purpose: To compare the mean keratometry measured (KA) in children under anesthesia to receive surgical treatment for congenital or developmental cataract with a mean keratometry obtaeined by a theoretical formula (KC), using the implanted intraocular lens (IOL) power value and postoperative refractional and biometric data, in order to check possible observational erros of KA due to general anesthesia. Methods: A retrospective study analysing records of patients with congenital or developmental bilateral cataract who underwent surgical treatment at HC-FMB. Seventy-three children’s eyes that had at least one full postoperative exam were analyzed, each time one eye was assessed was considered one element of the sample group, and the total amount was 165 elements. KC was determined by a theoretical formula for calculating IOL power using postoperative biometric data (AL e ACD), postoperative automatic refractometery and refractive power of the implanted IOL. The KA observational error value was obtained by subtracting KC from KA (Error = KA - KC). Statistical analysis of the observational erros and the comparison between KA and KC were made by Goodman’s test and Kruskal-Wallis’ non-parametric test. Results: the mean age at the moment of the first surgery was of 954,62 days, the standard deviation was ±794,14 days, the median was 953 days, the minimum age was 44 days and the maximum age was 2659 days. Twenty-three (62,16%) patients were male and 14 (37,84%) female. KA ranged from 40,62D to 51,50D, the median was 45,25D, the mean was 45,32D and the standard deviation was ±2,37D. KC ranged from 39,40D to 52,26D, the median was 44,49D, the mean was 44,54D and the standard deviation was ±2,41D. Observational errors ranged from -2,28D to 3,81D, the median was 0,83D, the mean was 0,79D and the standard deviation was ±1,18D. The rate between KC and KA can be represented by the equation KA = 1.0172 KC. Conclusion: The comparison between KC and KA in children under general anesthesia showed that there is an overestimation of the value measured when compared to the calculated one. The analysis of the observational errors showed there is a tendency to greater overestimation the higher the KA, with significant difference (p <0.05) in the measurements over 44.0D.

Orientador: Antonio Carlos Lottelli Rodrigues / Resumo: Objetivo: Comparar a ceratometria média aferida (KA) sob narcose em crianças submetidas à facectomia, por catarata congênita ou do desenvolvimento, com a ceratometria média obtida por cálculo teórico (KC), utilizando o poder dióptrico da lente intraocular (LIO) implantada e dados refracionais e biométricos pós-operatórios, a fim de evidenciar possíveis erros de aferição da KA devido a narcose. Métodos: Estudo retrospectivo realizado a partir da análise de dados coletados de prontuários de pacientes com catarata bilateral, congênita ou do desenvolvimento, que receberam tratamento cirúrgico no HC-FMB. Foram analisados 73 olhos de crianças que possuíam pelo menos um exame pós-operatório completo, cada momento em que determinado olho foi examinado foi considerado um elemento do conjunto amostral, totalizando 165 momentos. KC foi obtida a partir de fórmula teórica para cálculo de LIO, utilizando os dados biométricos pós-operatórios (AL e ACD), refratometria automatizada pós-operatória e poder dióptrico da LIO implantada. Foi calculada a diferença entre KA e KC (Desvio = KA – KC). Para análise estatística dos desvios encontrados e comparação entre KA e KC, foi realizado o teste de associação de Goodman e o teste não paramétrico de Kruskal-Wallis. Resultados: A média de idade no momento da cirurgia do primeiro olho foi 954,62 dias, com desvio padrão de ±794,14 dias, mediana de 953 dias, sendo a idade mínima de 44 dias e a máxima de 2659 dias. Vinte e três eram do sexo masculino (62,... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Purpose: To compare the mean keratometry measured (KA) in children under anesthesia to receive surgical treatment for congenital or developmental cataract with a mean keratometry obtaeined by a theoretical formula (KC), using the implanted intraocular lens (IOL) power value and postoperative refractional and biometric data, in order to check possible observational erros of KA due to general anesthesia. Methods: A retrospective study analysing records of patients with congenital or developmental bilateral cataract who underwent surgical treatment at HC-FMB. Seventy-three children’s eyes that had at least one full postoperative exam were analyzed, each time one eye was assessed was considered one element of the sample group, and the total amount was 165 elements. KC was determined by a theoretical formula for calculating IOL power using postoperative biometric data (AL e ACD), postoperative automatic refractometery and refractive power of the implanted IOL. The KA observational error value was obtained by subtracting KC from KA (Error = KA - KC). Statistical analysis of the observational erros and the comparison between KA and KC were made by Goodman’s test and Kruskal-Wallis’ non-parametric test. Results: the mean age at the moment of the first surgery was of 954,62 days, the standard deviation was ±794,14 days, the median was 953 days, the minimum age was 44 days and the maximum age was 2659 days. Twenty-three (62,16%) patients were male and 14 (37,84%) female. KA ranged from... (Complete abstract click electronic access below) / Mestre

L'objectif de la thèse était de déterminer les paramètres impactant la croissance des bulles dans des fromages à pâte pressée non cuite et de les hiérarchiser. Ce travail s'est appuyé sur la confrontation de données complémentaires telles que la composition, les propriétés rhéologiques, le métabolisme bactérien, la microstructure et l'ouverture des fromages. La plupart des méthodologies mises en place étaient originales, notamment l'imagerie 3D couplée à un traitement d'images permettant d'obtenir le nombre et le volume des yeux mais aussi leur localisation dans les fromages et leur cinétique individuelle de croissance. Or un fort gradient de taille et de cinétique de croissance a été mis en exergue entre le centre et la périphérie des fromages, avec des bulles moins nombreuses, plus petites et croissant moins vite en périphérie qu'à cœur. Plusieurs pistes ont été explorées pour expliquer ces disparités. La microstructure des deux zones n'ayant pas présenté de différence significative, les seuls paramètres en mesure de provoquer le gradient d'ouverture étaient la teneur en sel et les propriétés rhéologiques. En effet, la périphérie des fromages est plus salée et plus dure que le centre. La combinaison d'IRM et de respirométrie a permis de démontrer que le sel influence le gradient d'ouverture des fromages via la production de CO2 en ralentissant la croissance et le métabolisme des bactéries et potentiellement via sa solubilité. La dureté plus élevée de la périphérie des fromages pourrait accentuer les écarts. Le facteur clef de contrôle du gradient d'ouverture dans les fromages type PPNC emballés serait donc le sel, bien que les étapes de procédé précédant l'affinage semblent également être un paramètre intéressant à étudier car elles déterminent l'état initial des fromages en entrée d'affinage. / The main objective of the PhD was to determine and prioritize the factors affecting the eye growth in semi-hard cheeses. To do so, a combination of protocols dedicated to the analysis of specific elements of cheese was developed: cheese composition, microstructure, and rheological properties, opening indicators and bacteria metabolism. Several methods were innovating, especially the 3D imaging techniques combined with an image processing allowing the determination of eye’s number and volume but also their location in cheese and their individual specific growth during ripening. Therefore, a strong opening gradient could be identified between under-rind and core zones, with smaller and fewer eyes under-rind, which were growing slower than in the center. Several leads were investigated to explain these differences. The microstructure of the cheese matrix in these two zones did not present any significant difference, so the only parameters left that could cause the opening gradient were the salt content and the rheological properties. Indeed, the salt content and the firmness were higher under-rind than in the center of cheese. The combination of MRI and gas pressure measurement demonstrated that the salt content influences the eye growth gradient through the CO2 production by slowing down the growth and metabolism of bacteria, and maybe also through its solubility in the cheese matrix. The higher firmness under-rind could increase the opening gradient. Therefore, the key factor to control the opening gradient in packaged semi-hard cheeses would be the salt content, even if the process steps occurring before ripening could also be interesting to study, as they set the initial state of cheeses entering ripening.

<p>Stopa prematuriteta konstantno raste, a sve vi&scaron;e prevremeno rođenih beba koje prežive neonatalni i perinatalni period zahteva adekvatno sistemsko i oftalmolo&scaron;ko praćenje i tretman. Prematuritet sam po sebi, predstavlja rizik za razvoj vida. Taj rizik dodatno povećava prisustvo prematurne retinopatije i refraktivnih mana. Skrining i tretman promena na retini u sklopu prematuriteta, kao i optička korekcija refraktivnih mana obezbeđuju uslove za razvoj vida. Cilj ovog istraživanja je bio utvrditi promene biometrijskih karakteristika oka prematurusa, sa i bez prematurne retinopatije, tokom &scaron;estogodi&scaron;njeg praćenja, utvrditi refraktivni status ove dve grupe dece sa &scaron;est godina, te povezati promene biometrijskih karakteristika oka sa refrakcijom. U ispitivanje je uključeno 192 prevremeno rođena deteta (384 oka). Vr&scaron;ena su tri pregleda, u uzrastu od 3 meseca, 12 meseci i 6 godina starosti. Nakon prvog pregleda ispitivani uzorak je, u zavisnosti od nalaza na očnom dnu, podeljen na bebe sa prematurnom retinopatijom i bebe bez ovog oboljenja, a kako bi se uporedile proučavane karakteristike. U sva tri navedena uzrasta je pregledano očno dno i merene su tri glavne biometrijske karakteristike koje utiču na refraktivni status: aksijalna dužina oka, dubina prednje očne komore i debljina očnog sočiva. Sa &scaron;est godina je određena refrakcija, uključujući zakrivljenost rožnjače odnosno kornealni astigmatizam, vidna o&scaron;trina bez korekcije refraktivne mane, a zatim i nakon korekcije. Ostali neophodni podaci su dobijeni iz medicinske dokumentacije. Pokazano je da prematurna retinopatija utiče na refraktivni status i vidni ishod sa &scaron;est godina, pre svega uticajem na zakrivljenost rožnjače, aksijalnu dužinu oka i dubinu prednje očne komore, dok su promene debljine očnog sočiva bez posebnog značaja. Vidne o&scaron;trine oba oka u pred&scaron;kolskom uzrastu prevremeno rođene dece sa prematurnom retinopatijom su statistički značajno manje kada se uporede sa vidnim o&scaron;trinama prevremeno rođene dece bez retinopatije, ali razlika u ovim dvema vrednostima gubi statističku značajnost nakon optičke korekcije. Učestalost astigmatizma u prematurnoj populaciji sa 6 godina je visoka, a od onih koji imaju astigmatizam u tom uzrastu, vi&scaron;e od dve trećine je imalo prematurnu retinopatiju. Ukupna učestalost miopije, hipermetropije i emetropije u prematurnoj populaciji sa 6 godina je 18,9%, 54,7% i 13,2%, a preostalih 13,2% su anizometropi. U vi&scaron;e od trećine dece, sa prematurnom retinopatijom u najranijem uzrastu, sa &scaron;est godina postoji gubitak vidne sposobnosti, koji je najče&scaron;će blag, no može biti i potpun.</p> / <p>The number of babies born prematurely is constantly growing, so more and more of them need appropriate systemic and ophthalmologic monitoring and treatament. Prematurity itself, represents a risk for the development of vision. This risk further reinforces the presence of retinopathy of prematurity and refractive errors. Screening and treatment of retinopathy, as well as optical correction of refractive errors provide adequate conditions for the development of vision. The aim of this study was to determine changes of biometric features of eyes of the prematurely born infants, with and without retinopathy of prematurity, during the six-year follow-up. We also want to determine the refractive status of these two groups of children in the six year, and to link changes of biometric features with their eye refraction. The study included 192 prematurely born children (384 eyes). The examinations were performed at 3 months, 12 months and 6 years and they included fundus examination and measurements of the ocular axial length, anterior chamber depth and lens thickness. After dividing the subjects into two groups, based on the results of the fundus examination at 3 months, the results of the children with and without retinopathy were compared. At the age of six we also determine the refraction of eyes, including the curvature of the cornea and corneal astigmatism, visual acuity without correction of refractive error, and then after it. Other necessary data were obtained from medical documentation. It is shown that retinopathy of prematurity affects the refractive status and visual outcome at sixth year, primarily by the changes of curvature of the cornea, the axial length of the eye and the depth of the anterior chamber, while the change in the lens thickness has no significance. Visual acuity in both eyes in the preschool age preterm born children with retinopathy of prematurity are significantly less, when compared to visual acuity in preterm infants without retinopathy, but the difference in these two values lost statistical significance after optical correction. The rate of astigmatism in preterm population of six years is high, and of those who have astigmatism in this age group, more than two-thirds had retinopathy of prematurity. The overall incidence of myopia, hypermetropia and emetropia in the prematurely born, six years old children is 18.9%, 54.7% and 13.2%, and the remaining 13.2% have anisometropia. In more than a third of children with retinopathy of prematurity at an early age, with six years, there has been loss of visual acuity, which is usually mild, but it can be complete.</p>

Die Arbeit umreißt das Krankheitsbild der Myopie (Kurzsichtigkeit) und deren unterschiedliche Ausprägungen, im Speziellen der progressiven und pathologischen Myopie. Hierbei wird ein Einblick in die Symptomatik, die anatomischen Ursachen und die heutigen medizinischen Interventionen gegeben. Hierdurch wird die Problematik einer zu „weichen“ Sklera (Lederhaut des Auges) und des damit einhergehenden fortschreitenden Augenwachstums deutlich. Im Zentrum der Arbeit steht ein neuer therapeutischer Ansatz zur vorbeugenden Behandlung der pathologischen Myopie; das Riboflavin/Blaulicht Cross-Linking der Sklera des Kaninchenauges. Dessen Wirkungsweise ermöglicht die biomechanische Versteifung von kollagenem Gewebe. Aus diesem Sachverhalt ergibt sich die Fragestellung der Arbeit: Ist das sklerale Riboflavin/Blaulicht Cross Linking geeignet das Augenwachstum im Tiermodell (junge Kaninchen) verträglich zu hemmen? Operationsbeeinflussende Parameter wie die Riboflavin-Durchdringungsdauer der Sklera und die sklerale Lichtdurchlässigkeit werden untersucht und für die Optimierung der Operationsmethode herangezogen und diskutiert. Zur Einschätzung des Versuchsansatzes werden die im Methodikteil dargelegten Anwendungen an adulten und jungen Kaninchen/Kaninchenaugen durchgeführt. In Tierversuchen wird die Schadensschwelle in Abhängigkeit der Blaulichtintensität, des Alters und der Pigmentierung untersucht, wobei histologische, immunhistochemische und elektronenmikroskopische Verfahren angewendet werden. Der inhibitorische Einfluss des Riboflavin/Blaulicht Cross-Linkings auf das Augenwachstum kann im Jungtiermodell durch verschiedene metrische Verfahren und MRT-Untersuchungen belegt werden.