<p>As a part of the fight against the global warming the energy production needs to be more efficient and redirected towards sustainable options. One alternative is cogeneration, which means that electricity and heat is produced in one plant. The purpose with this survey is to examine if there are any commercial available combined heat and power techniques, based on combustion of solid moist biomass, which are suitable to small-scale applications. The technique must be able to produce between 2 and 10 MW thermal and the heat demand is a Swedish district-heating system. When already published reports had been studied, the Organic Rankine Cycle (ORC) was chosen as the most suitable technique. The possibility of using the ORC to generate electricity from the district-heating return flow was considered simultaneously. The chosen ORC-technique was then evaluated in Excel. The first aspect to be examined was how the performance of a combined heat and power plant was affected by variations in the supply line temperature. It showed that the performance reaches top levels when the temperature is low. The second part contains an optimisation, in a techno-economical perspective, of the ratio between cogeneration and separate heat production for district-heating systems with heat demands below 50 GWh/year. The most profitable combined heat and power plant generates 45 % of the installed power in a 50 GWh system. The profit is, however, too low to justify any construction plans. The conclusion was that there are no economical reasons to choose combined heat and power based on an organic rankine cycle in Sweden today.</p>

As a part of the fight against the global warming the energy production needs to be more efficient and redirected towards sustainable options. One alternative is cogeneration, which means that electricity and heat is produced in one plant. The purpose with this survey is to examine if there are any commercial available combined heat and power techniques, based on combustion of solid moist biomass, which are suitable to small-scale applications. The technique must be able to produce between 2 and 10 MW thermal and the heat demand is a Swedish district-heating system. When already published reports had been studied, the Organic Rankine Cycle (ORC) was chosen as the most suitable technique. The possibility of using the ORC to generate electricity from the district-heating return flow was considered simultaneously. The chosen ORC-technique was then evaluated in Excel. The first aspect to be examined was how the performance of a combined heat and power plant was affected by variations in the supply line temperature. It showed that the performance reaches top levels when the temperature is low. The second part contains an optimisation, in a techno-economical perspective, of the ratio between cogeneration and separate heat production for district-heating systems with heat demands below 50 GWh/year. The most profitable combined heat and power plant generates 45 % of the installed power in a 50 GWh system. The profit is, however, too low to justify any construction plans. The conclusion was that there are no economical reasons to choose combined heat and power based on an organic rankine cycle in Sweden today.

District heating covers around 80 % of the heating demand in apartment buildings and 10 % in single-family homes in Sweden. In the year 2010 the total input of energy to the Swedish district heating grids was about 70 TWh, which corresponds to 11 % of the total energy input to the whole energy system in the country. Through the combined production of heat and power the CHP-plants often reaches an efficiency of 90 % from energy in the fuel to produced heat and power. In order to keep the efficiency high it is important for the owners of the plant to have good knowledge about the plants performance at different operation points. Strängnäs Energi AB initiated this diploma work together with FVB Sverige ab in order to come up with a program for short term planning of the production of heat and power at the company´s CHP-plant. The work consists of three main parts. The first part is an introduction to district heating and optimization of such systems and it also handles a short explanation of the district heating system in Strängnäs. The second part covers the performance tests that have been done at Strängnäs Energi AB´s CHP-plant. The performance tests were carried out in order to investigate how the plant is operating. The results from the tests show that the efficiency of the plant is very poor. This has its explanation in the losses in forms of combustible content in the bottom ash, which in some cases reaches over 4 %. The problems with the combustion have been known for a while at Strängnäs Energi AB, but they did not have any calculated value of the efficiency. The company is working hard in order to come up with good solutions for the combustion and therefore this is not included in the diploma work. Instead the third part of the diploma work is focused on developing of a model for short term production planning. The model was made in MS Excel and it consists of a number of power and mass balances over one day. The key to the calculations in the model is the calculation of the alfa-value. The alfa-value together with the power in the hot water condenser gives an iterative calculation. The hot water accumulator is then either charged or discharged depending on current electrical price, demand of district heating and the level in the accumulator. The results from the simulations done with the model shows that it is possible for Strängnäs Energi AB to earn between 0 – 20 000 SEK during one day if they operate their plant as suggested in the model.

The development of a vasculature within a tissue-engineered construct is one of the largest hurdles to successful bone regeneration. This thesis investigates methods to increase vasculature of such transplanted constructs, based on in vivo transplant studies and in vitro analysis of cell behaviors. A syngeneic mouse model in immunocompetent mice was developed and analyzed for both osteogenesis and hematopoiesis. This study demonstrates that syngeneic bone marrow stromal cells (BMSCs) are not rejected by the host, provided the strain of mice is sufficiently inbred. Additionally, an effective protocol was developed for the isolation of endothelial cells (ECs) from the bone marrow of mice. Two different sets of materials for this study were analyzed, both collagen based, and the GelfoamTM scaffold was found to possess advantages over synthesized collagen or collagen/hydroxyapatite composites, although only for mouse and not human bone transplantation. In order to gain rapid and integrated vasculature formation within the transplant, attempts were made to increase both (de novo) vasculogenesis and angiogenesis (ingrowth) from the surrounding tissue. For the former, transplant studies were combined with in vitro osteogenic calcification studies. Direct co-culture of the BMSCs and ECs increased osteogenic calcification and was monitored by using both alizarin red S quantification and quantitative polymerase chain reaction. Angiogenesis (as assessed by cell migration) was studied by various motility and chemotaxis assays in vitro, as well as through use of a directed in vivo angiogenesis assay. Growth factors, particularly TGF-β1 and BMP-4, were found to increase cell movement in these systems. In conclusion, we show that although much work remains to be done in order to increase the vasculature in bone transplants, systematic combination of in vivo and in vitro assays can elucidate the nature behind this crucial process in this context.