This thesis describes a detailed study of advanced fibre optic sensors and their applications for label-free biochemical detection. The major contributions presented in this thesis are summarised below. A self-assembly based in-situ layer-by-layer (i-LbL) or multilayer deposition technique has been developed to deposit the 2D material nanosheets on cylindrical fibre devices. This deposition technique is based on the chemical bonding associated with the physical adsorption, securing high-quality 2D materials coating on specific fibre cylindrical surface with strong adhesion as well as a prospective thickness control. Then a " Photonic-nano-bio configuration", which is bioprobes immobilised 2D-(nano)material deposited fibre grating, was built. 2D material overlay provides a remarkable analytical platform for bio-affinity binding interface due to its exceptional optical and biochemical properties. EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) and NHS (NHydroxysuccinimide) were used to immobilise bioprobes. This kind of configuration is considered to have many advantages such as: enhanced RI sensitivity, enrich immobilisation sites, improved binding efficiency, selective detection. Followed by this configuration, several label-free biosensors were developed. For example, graphene oxide coated dual-peak long period grating (GO-dLPG) based immunosensor has been implemented for ultrasensitive detection of antibody/antigen interaction. The GO-LPG based biosensor has been developed for label-free haemoglobin detection. Apart from biosensors, the black phosphorus (BP) integrated tilted fibre grating (TFG) has been proposed, for the first time, as BP-fibre optic chemical sensor for heavy metal (Pb2+ ions) detection, demonstrating ultrahigh sensitivity, lower limit of detection and wider concentration range. Ultrafast laser micromachining technology has been employed to fabricate long period grating (LPG) and microstructures on optical fibre. The ultrafast laser micromachined polymer optical fibre Bragg grating (POFBG) has been developed for humidity sensing, showing the significant improvement with the reduced response time.

La mise en forme spatiale de faisceau laser, en particulier l’obtention d’un profil d’intensité homogène intéresse aussi bien la recherche que l’industrie (recherche biomédicale, microscopie, découpe, gravure, marquage laser, Laser MegaJoule…). De par ses avantages intrinsèques, nous sommes désireux d’apporter une solution fibrée, monomode et à maintien de polarisation. Ces travaux s’articulent autour de 2 problématiques :L’obtention d’un mode plat polarisé linéairement.Différentes solutions ont été mises en place pour satisfaire cette contrainte (embouts, fibres air-silice et fibres toute solide incluant des barreaux de contraintes). Nous avons notamment réalisé une fibre microstructurée air-silice monomode délivrant un mode plat de diamètre 20µm à 1050nm de polarisation linéaire (taux d’extinction de 20dB, biréfringence ~0,6x10-4). Cette fibre a été intégrée avec succès dans une chaine amplificatrice délivrant un faisceau cohérent avec un profil d’intensité aplati polarisé linéairement dépassant 100µJ en régime nanoseconde. En parallèle le développement de codes numériques a permis de proposer des designs augmentant la biréfringence voire polarisants.L’augmentation de l’aire effective du mode.Nos études ont permis de mettre en évidence les compromis entre qualité modale et pertes par courbures y compris dans le cas de structure présentant des résonateurs pour « vider » les modes d’ordre supérieur. L’impact sur le contenu modal des indices de différents types de barreaux de contraintes et de la biréfringence induite a également été étudié. Enfin nous avons réalisé une fibre mode plat à 1050nm de diamètre 34µm (aire effective ~1200µm2) utilisable en tant qu’embout. / Spatial beam shaping, in particular an homogeneous intensity profile is very attractive to fundamental research and industry (biomedical, microscopy, laser cutting, engraving, marking, Laser MegaJoule…). Thanks to its intrinsic advantages we wish to offer a fibered, single-mode and polarization maintaining solution. This work addresses two difficulties:Producing singlemode fiber delivering a flat mode while preserving the light polarization.Several solutions were developed to achieve this objective: fiber end-cap, air-silica and all-solid microstructured fibers with Stress Applying Parts (SAP). We have obtained several fibers including an air-silica microstructured single-mode fiber which delivers a flat fundamental mode with a diameter of 20µm at 1050nm linearly polarized (polarization extinction ratio of 20dB and a birefringence of 0.6x10 4). This fiber was successfully integrated in an amplifier chain delivering a coherent output beam with a flat intensity profile linearly polarized with a power of more than 100µJ for 10 ns pulses. In the same time, we developed a numerical code enabling us to propose designs with enhanced birefringence and even a polarizing behavior.Increase of mode effective areaOur studies exhibit the compromises required between modal quality and bending losses even in the case of a fiber design with resonator to extract from the core its high order modes. The impact of indices of different kinds of SAP and the impact of the induced birefringence on the modal content are also studied. Finally we realized a fiber delivering a flat intensity with a mode diameter equals to 34µm (effective area ~1200µm²) at 1050nm which can be used like an end-cap.

Innovative new flooring systems utilising lightweight fibre reinforced polymer composite materials may have the significant potential to offer both economic and performance benefits for infrastructure asset owners compared to conventional concrete and steel systems. Over recent years, a range of prototype floor systems using fibre reinforced polymer composites have been developed by researchers at the University of Southern Queensland. However before such structural systems can be widely adopted by industries, fundamental understanding of their behaviour must be improved. Such work will allow for the development of new design and analysis procedures which will enable engineers to efficiently and accurately design and analyse such structures. This dissertation presents an investigation into a new two-way fibre reinforced composite floor slab system. The proposed new two-way slab system is, in essence, a sandwich structure with an innovative hollow core made from a castable particulate filled resin system. The key focus of this dissertation is the development of a new analysis tool to analyse the two-way fibre reinforced composite slab and facilitate subsequent parametric studies into slab configurations for concept refinement. The detailed 3D finite element analyses and experimental investigations are performed to verify the new analysis tool, and provide more detailed insight into the structural behaviour of this new two-way fibre reinforced composite slab. Comparisons with detailed 3D FEA and experiments illustrate that the simplified analysis tool is capable of providing sufficient accuracy for the preliminary analysis of a slab structure. Moreover, the 3D finite element analyses agree well with the experiments, and it is concluded that the behavioural responses of the proposed new slab structure can be reliably predicted. The experimental results show that this new slab concept exhibits quite a robust static behaviour and is likely to have a robust fatigue performance.

Composite materials have become viable alternatives to traditional engineering materials for many different product categories. Liquid transfer moulding (LTM) processes, specifically resin transfer moulding (RTM), is a cost-effective manufacturing technique for creating high performance composite parts. These parts can be tailor-made to their specific application by optimizing the properties of the textile preform. Preforms which require little or no further assembly work and are close to the shape of the final part are critical to obtaining high quality parts while simultaneously reducing labour and costs associated with other composite manufacturing techniques. One type of fabric which is well suited for near-net- shape preforms is stitched non-crimp fabrics. These fabrics offer very high in-plane strength and stiffness while also having increased resistance to delamination. Manufacturing parts from these dry preforms typically involves long-scale fluid flow through both open channels and porous fibre bundles. This thesis documents and analyzes the flow of fluid through preforms manufactured from non-crimp fabrics featuring through-thickness stitches. The objective of this research is to determine the effect of this type of stitch on the RTM injection process. All of the tests used preforms with fibre volume fractions representative of primary and secondary structural parts. A series of trials was conducted using different fibre materials, flow rates, fibre volumes fractions, and degrees of fibre consolidation. All of the trials were conducted for cases similar to RTM. Consolidation of the fibres showed improvements to both the thoroughness of the filling and to the fibre volume fraction. Experimentally determined permeability data was shown to trend well with simple models and precision of the permeability data was comparable to values presented by other authors who studied fabrics which did not feature the through-thickness stitches.

Constipation is a serious problem in the pediatric population and often requires medical management with laxatives and enemas. Participants (2-10 years of age, n=13) with a history of mild constipation were assigned randomly to a fibre treatment or placebo group. After three weeks, subjects were crossed over to the other treatment. Pea hull fibre (4.0-7.6 g/day = 3.6-6.8 g/day of dietary fibre) was added to snack foods and an inulin supplement (5.0 g/day = 4.5 g/day of dietary fibre) was given, whereas the placebos were non-fortified snacks and maltodextrin (5.0 g/day).<p> Subjects or their parents documented stool frequency, stool consistency, occurrence of abdominal pain and intake of snack foods and the supplement. Over the final two weeks, there was a trend towards an increase in the mean number of daily bowel movements in the fibre treatment group compared to the placebo group (n=11, 0.68 ± 0.18 vs. 0.59 ± 0.26, p=0.064). Exclusion of one subject with diarrhea-type stools led to a significant difference between groups (n=10, 0.54 ± 0.18 vs. 0.67 ± 0.22, p=0.002). Stool consistency, using the Bristol Stool Form Rating Scale, showed no significant differences in stool consistency between groups (p=0.379) nor was there a difference in the incidences of abdominal pain (p=0.129). Not all subjects experienced abdominal pain. The inulin supplement (91% compliance rate; 1 serving per day) was consumed more consistently than were the snack foods fortified with pea hull fibre (77% compliance rate; 2 servings per day). There were no significant differences in the intake of the snacks or supplement when the placebo and treatment groups were compared. Energy intake was significantly lower during the fibre treatment period compared to placebo (n=12, 1307 ± 296 kcal/day vs. 1441 ± 285 kcal/day, p=0.035). The addition of pea hull fibre to typical snack foods and an inulin supplement to beverages were well accepted by children and no adverse effects were reported. Fibre fortification of snack foods with pea hull fibre and fibre supplementation of beverages with inulin may provide an alternative means to treat pediatric constipation.

The large amount of used polypropylene baler twine generated from the agricultural community may provide a low-cost, environmentally friendly source of fibre reinforcement that can be used to improve the properties of concrete. However, the performance of such fibres for the application has not yet been explored. The effectiveness of using small amounts of chopped baler twine to control the restrained plastic shrinkage cracking of portland cement mortar was investigated in this study. To determine the influence of baler twine fibre type, length and volume fraction on their performance, two types of baler twine ( one composed of strands with circular cross section, the other composed of flat band shape strands) in two lengths (19 mm and 38 mm) and three volume fractions (0.05%, 0.1%, and 0.3%) were evaluated. To compare the performance of baler twine fibre with that of other commercially available synthetic fibres, fibrillated polypropylene fibres at equal lengths and volume fractions was investigated.<p>The restrained plastic shrinkage tests were carried out by subjecting the fibre-reinforced mortar specimens, cast on rough substrate bases, to a wind speed of 2.6 m/s, and relative humidity less than 3% at 35 °C for 22 hours. To evaluate the effectiveness of the fibres, the crack numbers were recorded, and the maximum crack width and total crack area on the surface of each specimen were measured using an image analysis technique. Unrestrained plastic shrinkage tests were also conducted in which fibre-reinforced mortar specimens without the substrate bases were tested under the same environmental conditions.<p>Test results indicate that both types of baler twine are capable of controlling restrained plastic shrinkage cracking to some extent, but are not as effective as fibrillated polypropylene. The baler twine composed of band shape strands performed better than the one composed of strands with circular cross section. Compared with plain specimens, the total crack area was reduced by 95.3, 77.5 and 38.7% when 0.3% volume fraction of 38 mm fibrillated polypropylene, band shape baler twine and circular baler twine fibres, respectively, were added. Similar reductions in maximum crack width were observed. Fibre length did not significantly influence cracking behaviour. Free plastic shrinkage was significantly reduced only when long fibre lengths (38 mm) and high volume fractions (0.3%) were used.

The large amount of used polypropylene baler twine generated from the agricultural community may provide a low-cost, environmentally friendly source of fibre reinforcement that can be used to improve the properties of concrete. However, the performance of such fibres for the application has not yet been explored. The effectiveness of using small amounts of chopped baler twine to control the restrained plastic shrinkage cracking of portland cement mortar was investigated in this study. To determine the influence of baler twine fibre type, length and volume fraction on their performance, two types of baler twine ( one composed of strands with circular cross section, the other composed of flat band shape strands) in two lengths (19 mm and 38 mm) and three volume fractions (0.05%, 0.1%, and 0.3%) were evaluated. To compare the performance of baler twine fibre with that of other commercially available synthetic fibres, fibrillated polypropylene fibres at equal lengths and volume fractions was investigated.<p>The restrained plastic shrinkage tests were carried out by subjecting the fibre-reinforced mortar specimens, cast on rough substrate bases, to a wind speed of 2.6 m/s, and relative humidity less than 3% at 35 °C for 22 hours. To evaluate the effectiveness of the fibres, the crack numbers were recorded, and the maximum crack width and total crack area on the surface of each specimen were measured using an image analysis technique. Unrestrained plastic shrinkage tests were also conducted in which fibre-reinforced mortar specimens without the substrate bases were tested under the same environmental conditions.<p>Test results indicate that both types of baler twine are capable of controlling restrained plastic shrinkage cracking to some extent, but are not as effective as fibrillated polypropylene. The baler twine composed of band shape strands performed better than the one composed of strands with circular cross section. Compared with plain specimens, the total crack area was reduced by 95.3, 77.5 and 38.7% when 0.3% volume fraction of 38 mm fibrillated polypropylene, band shape baler twine and circular baler twine fibres, respectively, were added. Similar reductions in maximum crack width were observed. Fibre length did not significantly influence cracking behaviour. Free plastic shrinkage was significantly reduced only when long fibre lengths (38 mm) and high volume fractions (0.3%) were used.

Constipation is a serious problem in the pediatric population and often requires medical management with laxatives and enemas. Participants (2-10 years of age, n=13) with a history of mild constipation were assigned randomly to a fibre treatment or placebo group. After three weeks, subjects were crossed over to the other treatment. Pea hull fibre (4.0-7.6 g/day = 3.6-6.8 g/day of dietary fibre) was added to snack foods and an inulin supplement (5.0 g/day = 4.5 g/day of dietary fibre) was given, whereas the placebos were non-fortified snacks and maltodextrin (5.0 g/day).<p> Subjects or their parents documented stool frequency, stool consistency, occurrence of abdominal pain and intake of snack foods and the supplement. Over the final two weeks, there was a trend towards an increase in the mean number of daily bowel movements in the fibre treatment group compared to the placebo group (n=11, 0.68 ± 0.18 vs. 0.59 ± 0.26, p=0.064). Exclusion of one subject with diarrhea-type stools led to a significant difference between groups (n=10, 0.54 ± 0.18 vs. 0.67 ± 0.22, p=0.002). Stool consistency, using the Bristol Stool Form Rating Scale, showed no significant differences in stool consistency between groups (p=0.379) nor was there a difference in the incidences of abdominal pain (p=0.129). Not all subjects experienced abdominal pain. The inulin supplement (91% compliance rate; 1 serving per day) was consumed more consistently than were the snack foods fortified with pea hull fibre (77% compliance rate; 2 servings per day). There were no significant differences in the intake of the snacks or supplement when the placebo and treatment groups were compared. Energy intake was significantly lower during the fibre treatment period compared to placebo (n=12, 1307 ± 296 kcal/day vs. 1441 ± 285 kcal/day, p=0.035). The addition of pea hull fibre to typical snack foods and an inulin supplement to beverages were well accepted by children and no adverse effects were reported. Fibre fortification of snack foods with pea hull fibre and fibre supplementation of beverages with inulin may provide an alternative means to treat pediatric constipation.

Composite materials are widely used in demanding applications in aerospace and other industries. In order to understand the complex behaviour of the composite materials and their components, standardised test methods are used. One example is the double cantilever beam (DCB) test in which the test specimen is loaded in an opening, i.e., tensile mode. Failures in composite materials loaded compression are different from those in tension, for example, kink band or buckling-like failures can occur. In this project, several DCBs are designed and a new fixture which allows for compression testing of a DCB is developed for an existing Instron testing machine. The fixture overcomes a known problem of tensile peak causing the failure of the adhesive at the inner surfaces of the DBC by applying additional compressive loads along the outer surfaces of the DBC. The compressive forces can induce the desired kink band formation so that researchers can better study the failure mode. The conceptual development of the new DCBs and the new fixture are presented. Several prototypes of the specimens and the fixture are modelled using the three-dimensional (3D) computer-aided design software Creo Parametric 2.0.  One of the fixtures is selected to further study. The different DCB specimens are studied in order to obtain information about the kink band using 3D finite element analysis with the software programme Abaqus CAE. The selected fixture is analysed to determine if there are any areas of concern. Finally, the behaviour of the compression stress along the DCB using two pairs of forces is studied. Unfortunately, it is determined that the tensile peak experienced by the adhesive cannot be eliminated by the application of two pairs of compressive loads, one at the free end and the other in the vicinity of the tensile peak. Several suggestions are made for future work which might serve to reduce the tensile peak; e.g., the movable force couple is applied as a surface load instead of a point load. For this, the fixture will have to be modified with a new geometry, although the DCB could be the same. This will allow further work to focus on the combined behaviour of the tensile peak and the fixture.

This work investigates the effects of long range hydrodynamic fibre-fibre interactions on the orientation state of a semi-dilute, rigid fibre suspension flowing through a linear contracting channel under laminar flow conditions. The effects of fibre-fibre interactions are modeled mathematically, the governing equations solved numerically and the predicted results compared with experimental observations. The theoretical model is based on the assumption that the orientation state of the suspension can be completely described by a probability distribution function and that fibre-fibre interactions are random in nature, thus giving rise to a diffusion-type process. The orientation distribution evolves spatially according to a Fokker-Plank type equation using closure equations for the rotary diffusion coefficient advanced by either (i) Folgar and Tucker (J. Reinforced Plast. Comp. 3 98–119 1984) or (ii) Koch (Phys. Fluids 7(8) 2086–2088 1995). Each of these two closure models for the rotary diffusion coefficient contains an unknown empirical constant that must be determined from experiments. These were fit to experimental data along the central streamline of the contraction as a function of fibre concentration. The diffusion coefficient was found to first increase with increasing suspension concentration up to a maximum, and then decrease with concentration above this point. This non-monotonic behavior was attributed to fibre flocculation, a mechanism not considered in the relationships for the rotary diffusion coefficient. The theoretical model is then extended to predict fibre orientation over the entire plane of the contraction and the two-way momentum coupling between the fluid and fibre phases were investigated numerically. The results show that the structure of the flow field within the contraction is significantly altered when the fibre phase is considered, demonstrating the non-negligible effect of the momentum exchange between the two phases. Comparison is made between the predicted orientation state of the suspension with experimental observations over the contraction plane. Good agreement was found between the model predictions and the experimental observations except in a small region near the solid boundaries. These near wall discrepancies were attributed to an inability to correctly handle the wall boundary conditions in the fibre orientation model.