Lodgepole pine (Pinus contorta Douglas ex Louden) is the most widespread pine in North America and the single most abundant tree species in British Columbia (BC). Its vast distribution, diversity and economic value make it an important species for timber harvest and subsequent reforestation. Climate change raises serious concerns over the adaptability and effective management of BC’s future forests. The majority of lodgepole pine seedlings requested for replanting are produced from seed obtained from wild stands, but the relationship between climate variation and the seed production of natural populations has yet to be assessed. The purpose of this study is to determine if variation in P. contorta seed yield is related to the climate of BC. Historical cone collection data were obtained from archived records of 1948 seedlots in 22 different natural stand seed planning zones (SPZs) of BC. Collections were made between 1963 and 2013 and seed yield (kg fresh seed/hL cone) was determined for each seedlot. First, natural variation in seed production of lodgepole pine was examined in 18 different SPZs. The Nass Skeena Transition (NST) represents a unique intersection between continental and maritime ecosystems and was found to have a significantly higher mean seed yield compared to all other zones, with the exception of Hudson Hope (HH). However, variance in seed yield for NST was found to be an order of magnitude higher than that of other SPZs, indicating that seed production in this region is exceptionally variable. These findings provide a valuable geographic baseline for the reproductive fitness of lodgepole pine, suggesting that climate adaptation and mitigation strategies for some areas of the province may need to be region-specific. In addition, the relationship between climate variation and the seed production of P. contorta in BC was evaluated. The climate of each region was described using dynamically-downscaled Global Circulation Model (GCM) and reanalysis climate output from the Canadian Regional Climate Model (CRCM). Annual, winter, and summer means were explored for each of the climate variables of interest: total precipitation (mm) and surface air temperature (°C). Temporal correlations between the mean annual seed yield anomaly and the anomaly of both climate variables were significant under a variety of climate schemes in a number of SPZs. Significant overall trends in climate variables were also captured using GCM-driven CRCM output. While these two analyses independently highlighted significant relationships between seed yield and climate, their joint implications were unclear. Shifts in the CRCM boundary conditions revealed that the results lacked robustness during the historical period, inhibiting the investigation of future projections. Ambiguous age ranges for each cone collection and temporal restrictions of the seed collection data may be partially responsible for these inconclusive results. Results from the first half of this thesis suggest that, with few exceptions, seed production is relatively stable across SPZs spanning a wide range of climate regimes. Thus, the investigation of the relationship between reproductive fitness and climate may be complicated by the extraordinary adaptability of lodgepole pine and the high genetic variation in natural populations. / Graduate

1. The success of invasive species is often thought to be due to release from natural enemies. This hypothesis relies on the assumption that species are regulated by top-down forces in their native range and implies that species are likely to be regulated by bottom-up forces in the invasive range. Neither of these assumptions has been consistently supported with insects, a group which include many highly destructive invasive pest species. 2. Winter moth (Operophtera brumata) is an invasive defoliator in North America that appears to be regulated by mortality in the larval stage in its invasive range. To assess whether regulation in the invasive range is caused by top-down or bottom-up forces, we sought to identify the main causes of larval mortality. 3. To measure the importance of different sources of winter moth larval mortality, we used observational and manipulative field studies to measure dispersal, predation, parasitism, disease. We measured the response of larval dispersal in the field to multiple aspects of foliar quality, including total phenolics, pH 10 oxidized phenolics, trichome density, total nitrogen, total carbon, and carbon-nitrogen ration. We also used manipulative laboratory studies to measure the presence of cannibalism and dispersal. 4. Tree-level declines in density were driven by density-dependent larval dispersal of early instars with very little mortality caused by other factors. Later instar larvae dispersed at increased rates from previously damaged vs. undamaged foliage, and field larval dispersal rates were related to proportion of oxidative phenolics in 2015, suggesting that larval dispersal may have been mediated by an induced decline in foliar quality. 5. We conclude that winter moth population densities are regulated in New England by density-dependent larval dispersal possibly mediated by phenolic oxidative capacity. The suggested role of host plant quality in mediating dispersal means that winter moth population densities in New England appear to be regulated by bottom up forces, aligning with the assumptions of the natural enemy release hypothesis. This is the first study known to the authors presenting data showing a negative effect on insect herbivore performance from pH 10 oxidized phenolics.

The control and eradication of the invasive biennial herb garlic mustard (Alliaria petiolata) and the restoration of invaded forest habitats present important linked challenges to land managers in North America. Removing garlic mustard by hand and by glyphosate herbicide application have both been used as eradication strategies with mixed results. Each method has advantages and disadvantages, but they are rarely compared for effectiveness and community impact across multiple years of management. Some previous studies have shown improvements in species diversity and plant community composition following management, while others have found no differences. To better understand both garlic mustard population and native plant community responses to these two methods across a broad geographic range, we tested these two management methods for four years in seven northern hardwood forests in Massachusetts and New York State. We found that pulling juvenile and adult garlic mustard plants for four years significantly reduced adult abundance, while spraying had no effect compared to invaded control plots. In the plant community, we found no negative impacts of garlic mustard on species diversity nor increased diversity in managed plots following three consecutive years of management. Our results suggest that increased diversity should not be the primary goal of garlic mustard management at these sites and plant community monitoring at the site-specific scale should be explored. This study highlights how complicated decisions can be for managers when deciding which invasions to prioritize and how to measure plant community recovery.

Anticipated climate change and increasing wood demand require dependable diameter growth models for adaptive forest management. We used a mixed-effects modeling approach with Forest Inventory and Analysis (FIA) data to fit diameter growth models for loblolly pine, other softwood species (slash pine, shortleaf pine, and longleaf pine), sweetgum, and other hardwood (southern red oak, red maple, and water oak) species. Climatic variables coupled with individual tree attributes and competition factors improved climate insensitive models. Growth of loblolly pine and sweetgum was positively correlated with mean temperature of the coldest month. Mean temperature of the warmest month negatively influenced diameter growth of loblolly pine and other hardwood species. Growing season precipitation and summer precipitation balance had negative effects on the growth of softwood and hardwood species, respectively. Inclusion of FIA plot as random effect improved model fit statistics and residual distribution of climate sensitive models. These findings will be useful to managers for recalibrating diameter growth models resulting in improved biomass yield and volume estimates that will better inform decisions.

Polyclonal plantings of Populus deltoides are expected to display increased site resource use, productivity, and tolerance to stress through plasticity changes leading to niche differentiation (i.e changes to crown/canopy structures). In the present study, P. deltoides Clones S7C8, 110412, and polyclonal plots were tested for differentially expressed genes and enriched biological pathways between planting schemes. Transcriptomic analysis of leaves revealed upregulation of an active growth gene and gene family members that play important roles in plant stress and stress tolerance in polyclonal plantings. A gene associated with oxidative stress was upregulated in polyclonal plantings across all treatments. Secondary metabolic pathways including arginine and proline metabolism were upregulated in monoclonal plantings and downregulated in polyclonal plantings. Phenotypic results displayed greater aboveground biomass in polyclonal plantings. Results suggested a potential increased tolerance in polyclonal plantings to water and heat stress, including increased productivity and resource usage.

Thesis TITLE: The Psárský forest in the district of Prague - West as a location of school science excursion AUTHOR: Bc. Michala Prokopcová DEPARTMENT: Department of Biology and Environmental Studies, Faculty of Education, Charles University SUPERVISOR: doc. RNDr. Vasilis Teodoridis, Ph.D. ABSTRACT The aim of the thesis is to examine the Psárský forest locality in the Prague-West region as regards its suitability for a school science excursion. The principal objective of the thesis was to prepare and take a comprehensive science excursion to Psárský forest and verify its educational efficiency for pupils of lower secondary school grades by means of pedagogical research. The research was carried out through didactical tests comparing the initial knowledge before the excursion (pretest) and acquired knowledge after the excursion (posttest). The didactical concept of the science excursion to Psárský forest was elaborated in accordance with the key aspects of the field biology didactics and based on complex evaluation of the fields of interest for the excursion planned to become a basis for the excursion contents and selection of suitable places for excursion activities. In addition to this, subsequent research via the attitude questionnaire to identify the excursions benefits within natural science education from...

In 1935 I first visited the Kings Canyon area. Since then I have spent many hours wandering through the woods and over the mountains in the area around the old General Grant National Park and the country which is now included in the Kings Canyon National Park. To my knowledge, no complete flora has been made for this vicinity. I consider the following pages to be the beginning of a fascinating work. It is the beginning because this paper includes only plants with woody stems.

Elevated atmospheric CO2 has been implicated as a driver of increased liana abundance worldwide. Known as disturbance creators and beneficiaries, lianas possess the potential to significantly influence forest ecosystems. I investigated the early-invasion dynamics of Euonymus fortunei (wintercreeper), an evergreen liana that is invading forests in eastern North America, disrupting native plant communities and ecosystem functions. Wintercreeper is widely cultivated as an ornamental groundcover, frequently invading natural areas via asexual stem growth. Invasion of remote natural areas is dependent upon seed transport and may occur less frequently. I examined the mechanisms of seed dormancy by conducting a ‘move-along’ experiment using fresh and after-ripened seeds. Additionally, I sought to characterize the nature of seed dispersal by birds by deploying seed traps within an invaded forest in central Kentucky. Wintercreeper seeds displayed conditional nondeep physiological dormancy. Although germination occurs at high velocity following cold stratification, a cold period was only facultative to break dormancy. While fresh seeds had greater germination rates (98.6%) compared to after-ripened seeds (85.7%), after-ripened seeds experienced earlier germination (41 days across all treatments). My findings also indicate that bird-mediated seed dispersal occurs throughout the winter from seeds that after-ripen on maternal liana phanerophytes. Overall, these data suggest wintercreeper seeds are capable of dispersing and recruiting in areas with mild to non-existent winters. The invasion of natural areas by exotic invaders is regulated by biotic and abiotic processes, which influence the invader’s success or failure. I studied the vegetation and soil effects of paired invaded (INV), uninvaded (NAT), and ‘restored’ (RES) sites on the germination and survival of wintercreeper seeds and seedlings. The effect of aril (with vs. without) was also tested, both in the field and in vitro. In the field, total germination and first year survival were 55.6% and 24.2%, respectively, across treatments. Total germination was unaffected by treatments, yet vegetation (P = 0.0016) and aril (P = 0.001) treatments significantly influenced germination rates over time, including delayed germination of seeds with arils. The proportion of germinated seedlings that survived was significantly different based on vegetation (P = 0.054) and aril (P = 0.071) treatments after the first winter of growth, but not prior to the first winter. The proportion of seedling survival was significantly lower among seeds dispersed with an aril and seeds sown within INV treatments; there were no interactive effects. Finally, I examined how seedling density and growth habit (horizontal vs. vertical) influenced plant survival, growth, and allometry. I also tested extractable soil C, N, P, K, Ca, and Mg prior to planting and after 17 months of soil conditioning. I found evidence that increased planting density negatively influenced growth among individual plants (lower survival, basal diameter, shoot mass, root mass). At the plot level, high-density plantings yielded greater stem length, and shoot, root, and combined biomass, indicating positive frequency dependence for this species. Soil analyses indicated C, N, P, Ca, and Mg significantly increased (P < 0.05) over the course of the experiment. I conclude that in wintercreeper, prominent asexual propagation leads to aggregate populations whose total contributions to above- and belowground biomass are positively correlated with density, even though individual plant fitness is not. Altogether, this work provides insight into how wintercreeper invasions occur at broad and fine scales. This information will provide a foundation for future wintercreeper studies and aid land managers in their prevention and control strategies.

Temperate forests are complex ecosystems that sequester carbon (C) in biomass. C storage is related to ecosystem-scale forest structure, changing over succession, disturbance, and with community composition. We quantified ecosystem biological and physical structure in two forest chronosequences varying in disturbance intensity, and three late successional functional types to examine how multiple structural expressions relate to ecosystem C cycling. We quantified C cycling as wood net primary production (NPP), ecosystem structure as Simpson’s Index, and physical structure as leaf quantity (LAI) and arrangement (rugosity), examining how wood NPP-structure relates to light distribution and use-efficiency. Relationships between structural attributes of biodiversity, LAI, and rugosity differed. Development of rugosity was conserved regardless of disturbance and composition, suggesting optimization of vegetation arrangement over succession. LAI and rugosity showed significant positive productivity trends over succession, particularly within deciduous broadleaf forests, suggesting these measures of structure contain complementary, not redundant, information related to C cycling.

Migration of animals has been studied for decades and has included everything from large terrestrial and pelagic mammals traveling thousands of kilometers to many types of birds flying through several countries to insects going through multiple life cycles in a single migration. The migration of bats has been studied in broad terms to gather coarse information such as distance traveled, connecting summer and winter habitat, and a general understanding of timing. However, only recently have researchers begun to understand the specifics of bat migration including physiology and fine resolution behavior. Using nine years of spring migration data collected on VHF radio-tagged federally endangered female Indiana bats (Myotis sodalis), I predicted behavior based on previous migration studies for bats that used various methods such as band recovery data, stable hydrogen isotopes, and automated telemetry. My project used aerial telemetry to collect location points while tracking individual bats throughout the migration. I describe distances traveled, how much time bats spent in migration, traveling and foraging behavior during migration, and how weather affects bat behavior. In addition, this project resulted in the location of 17 previously unknown maternity colonies for the species including the southernmost colony known to date. Next, I used temperature data collected from individual bats along with ambient temperature to determine how air temperature affects specific bat behavior. I calculated air temperature thresholds for use of torpor in Indiana bats and described how air temperature affects transition states between torpor and normothermy. Finally, I determined landscape preferences for migrating bats during travel and foraging. Indiana bats tend to migrate in a direct path from hibernaculum to summer grounds and use the habitat in the proportion that it is available. Although bats used forested cover when possible, they did travel in open areas (e.g., across agricultural fields) when necessary to continue along the intended trajectory. This data set is the first to track individual nocturnally migrating bats via aerial telemetry for the entirety of the spring migration journey. It provides specific information about how far Indiana bats travel and how long they are migrating across the landscape, illustrates a web of connections between summer and winter habitat, describes the effect of weather on bat behavior, and provides landscape use information that can be useful for land managers and developers.