When subtitles are burned into a video, an error can sometimes occur in the encoder that results in the same subtitle being burned into several frames, resulting in subtitles becoming frozen. This thesis provides a way to detect frozen video subtitles with the help of an implemented text detector and classifier. Two types of classifiers, naïve classifiers and machine learning classifiers, are tested and compared on a variety of different videos to see how much a machine learning approach can improve the performance. The naïve classifiers are evaluated using ground truth data to gain an understanding of the importance of good text detection. To understand the difficulty of the problem, two different machine learning classifiers are tested, logistic regression and random forests. The result shows that machine learning improves the performance over using naïve classifiers by improving the specificity from approximately 87.3% to 95.8% and improving the accuracy from 93.3% to 95.5%. Random forests achieve the best overall performance, but the difference compared to when using logistic regression is small enough that more computationally complex machine learning classifiers are not necessary. Using the ground truth shows that the weaker naïve classifiers would be improved by at least 4.2% accuracy, thus a better text detector is warranted. This thesis shows that machine learning is a viable option for detecting frozen video subtitles.

The consumption of seafood in the United States has increased rapidly in recent years due to high quality protein and health benefits of seafood. Seafood can be a carrier for bacteria normally distributed in the marine environment and, in some cases, can be contaminated by human pathogens. Therefore, there is a potential health risk if seafood is consumed raw or undercooked. However, information regarding prevalence of foodborne pathogens in retail seafood products and the ability of pathogens to survive in the products during refrigerated and frozen storage is limited. The objective of this study was to generate such information for a better understanding of distribution of foodborne pathogens in seafood products and provide data which might be used for risk assessment of foodborne infection associated with seafood consumption. A total of 45 seafood products were collected from local retail stores and analyzed for aerobic plate counts (APC) and psychrotrophic bacterial counts (PBC) as well as presence of foodborne pathogens, including Escherichia coli O157:H7, Salmonella, Listeria monocytogenes, Staphylococcus aureus, Vibrio parahaemolyticus, and Vibrio vulnificus according to procedures described in the U.S. Food and Drug and Administration Bacteriological Analytical Manual (BAM). Presumptive isolates for each foodborne pathogen were further characterized by biochemical reactions using commercial identification kits and confirmed with polymerase chain reaction (PCR) assay. The samples had bacterial populations ranging from 1.90 to 6.11 CFU/g for APC and from 2.00 to 6.78 CFU/g for PBC. According to the microbiological criteria of International Commission on Microbiological Specifications for Foods (ICMSF), all 45 samples were considered acceptable quality (APC < 10⁷ CFU/g, E. coli < 3 MPN/g) with most samples (93.3%) being good quality (APC < 5 × 10⁵ CFU/g, E. coli < 3 MPN/g). No E. coli O157:H7, Salmonella, S. aureus, V. parahaemolyticus, and V. vulnificus was detected in any samples. Two previously frozen shrimp products (4.4%) were confirmed to carry L. monocytogenes. Studies of growth and survival of L. monocytogenes (3 strains), S. aureus (2 strains), and Salmonella (2 serovars) in raw yellowfin tuna meat stored at 5 - 7 °C for 14 days revealed that L. monocytogenes had the ability to multiply in the tuna meat during refrigerated storage while populations of S. aureus and Salmonella were reduced by 1 to 2 log CFU/g after 14 days at 5 - 7 °C. Studies of holding raw yellowfin tuna meat contaminated with L. monocytogenes, S. aureus, and Salmonella at -18 ± 2 °C for 12 weeks observed that all three pathogens, except Salmonella Newport, in tuna samples survived the frozen storage with less than 2- log of reductions in the populations over 12 weeks of storage. No viable cell of Salmonella Newport was detected in samples after 42 days storage at -18 °C. Raw seafood can be a carrier of foodborne pathogens, particularly L. monocytogenes, and many foodborne pathogens can survive in frozen products for several months. Consumption of raw or undercooked seafood products may lead to human infection if the products are contaminated with pathogens. Therefore, sanitation standard operating procedure (SSOP), good manufacturing practice (GMP) and hazards analysis and critical control points (HACCPs) programs shall all be implemented in the seafood industry to prevent seafood products from being contaminated with foodborne pathogens during handling and processing. Moreover, proper storage of raw seafood products and avoiding cross-contamination during handling at the retail levels also helps to minimize risk of human infection associated with ready-to-eat products. / Graduation date: 2013

[pt] A presente dissertação de mestrado tem como objetivo principal apresentar à empresa Y qual a melhor forma de expandir o negócio de varejo de frozen yogurt no mercado do Rio de Janeiro. Para que este objetivo fosse alcançado, inicialmente foi estudada a indústria na qual o negócio de frozen yogurt, um tipo de fast food, está inserido, à luz da teoria de Michael Porter para análise estrutural de indústrias e da concorrência. Em seguida, buscou-se descrever as principais características de três modelos de expansão viáveis no caso: expansão por meio de operações próprias, por meio do sistema de franchising e por meio da forma plural. Este último modelo é um sistema híbrido, caracterizado pela coexistência de operações franqueadas e lojas próprias. Ao longo do trabalho são apresentados os principais pontos fortes e vulnerabilidades de cada um destes modelos aplicados ao negócio de frozen yogurt e, ao final, é sugerida qual a forma de expansão mais apropriada para o negócio. / [en] This paper has as main objective to suggest to company Y the best way on which to expand its frozen yogurt retail business in Rio de Janeiro s market. To reach this objective, it was initially studied the industry in which the frozen yogurt business, a kind of fast food, is inserted, based upon Michael Porter s theory of structural analysis for industries and competition. The next step was to describe the main features of three different viable expansion models, in this case: expansion through company-owned stores, through franchised stores or through a plural form. This last model, being a hybrid system, featured by the coexistence of franchised stores and company owned stores. Throughout this work the main strengths and vulnerabilities of each of these models applied to the frozen yogurt business are presented and in the end the most appropriate expansion strategy for the business is suggested, based upon the analysis and criteria adopted.

The frozen plasticity theory is a punctuationalist theory of adaptive evolution. It states that long periods of stasis, during which populations respond to selection pressures only by elastic change in the frequency of already present alleles, alternate in the evolution of sexual species with short periods of plastic evolution, during which alleles can get fixed or eliminated by directed selection. Asexual species are not expected to maintain such high genetic polymorphism in the long term. They should, however, be able to plastically respond to selection pressures throughout their whole existence. This difference between the evolutionary dynamics of sexual and asexual clades has a number of ecological and macroevolutionary implications. Concerning ecology, we could expect different environmental preferences of sexual and asexual species. Accordingly, in our first work that was based on a comparative study, we statistically significantly supported the hypothesis that (ancient) asexual groups of (eukaryotes) inhabit more stable and homogeneous habitats than their related sexual controls. Focusing on actually experienced, i.e. subjective, heterogeneity of the environment turned out to be the crucial factor of this type of research. From the viewpoint of macroevolutionary implications of the frozen...

Typescript (photocopy). / Digitized by Kansas Correctional Industries

Call number: LD2668 .T4 1985 C525 / Master of Science

A net shift analysis was used to analyze growth in employment and value added from 1954 to 1982 in SIC 2037, Frozen Fruits, Fruit Juices, and Vegetables. This analysis indicated that the Pacific Northwest dominated the growth experienced in this sector over this time period. Oregon's share of total U.S. employment in SIC 2037 increased from 7.3 percent in 1954 to 16.1 percent in 1982. Value added in Oregon was 16.1 percent of the U.S. total in 1982, and growth in both employment and value added was at a greater rate than the overall U.S. rate of growth in this sector. Washington's share of employment increased from 10.3 percent to 13 percent, and the share of U.S. total value added in this sector grew from 11 percent to 14.2 percent. Value added by the freezing of fruits and vegetables in Idaho increased to 10.3 percent of the U.S. total in 1982, and employment grew to a share of 10.5 percent, from 3.5 percent in 1954. An informal survey of executives in six food processing plants in Oregon suggested that expanding international export markets was essential to continued growth for this industry in the Pacific Northwest. A model of Japan's import demand of frozen vegetable products, specifically corn and potatoes, is estimated using data from 1978 through 1986 of real own price at the export site, Japanese consumer expenditures adjusted by Japan's CPI, and Japan's domestic production of frozen corn and potatoes. Commerce Department data on exports by Customs District was used to disaggregate import demand by region; namely Oregon, Washington, the two together as Pacific Northwest, and the total U.S. It was found that the demand for frozen corn imports is more responsive to changes in real own price than the import demand for frozen potatoes. Income elasticities were positive and higher for frozen potato import demand than for frozen corn demand, except for Oregon originating exports. Production in Japan of frozen corn has a higher negative impact on exports from Oregon ports than Washington based exports. Production of frozen potatoes in Japan did not have a negative impact on import demand from any of the four export sites. / Graduation date: 1989

A transmedial narrative is to tell a story over different mediums. It's a fairly new area that took its start in 2003 by Henry Jenkins. Since then has the interest of this new way to tell a story become more and more popular. But since the access to internet and media makes it possible to reach out to a much younger audience. The research needs to do that aswell. This is a case study that is aiming to find out how a transmedial narrative could be successfully designed for children. Through analysing and comparing two giants of transmedial storytelling who has their main target audience as children. Disney's Frozen and Cars. Through mapping of boths brand’s transmedial ecosystems, then analyse them with transmedial theories, and theories about narratives for children. This paper will in the end come up with four components that all needs to take into consideration when designing successfully a transmedial narrative for children. The four components are the following: World, Content, Characters and Accessibility.

The meat producers of the western world needs to develop their export organizations and to streamline their physical distribution in order to take new market shares on the fast growing overseas markets. HKScan is one of those meat producing companies, the group has businesses in Finland, Sweden, Denmark and the Baltic countries. A part of their sales goes frozen on overseas export by container sea freight. Lately the logistics management of HKScan has been interested in investigating the effects of centralizing the physical distribution for the overseas export from Sweden and Denmark. This leads to the purpose of this study, which is:  “For HKScan, develop and apply a model that evaluates distribution structures for overseas export of frozen food regarding total cost, delivery service, environmental impact and regulations.” The case study included comparison between the current distribution structure for HKScan and three pre-determined scenarios. The current setup consists of multiple warehouses in both countries. In the first scenario the distribution structure is centralized to include one warehouse per country. In the second scenario, the total export flow of products from both Sweden and Denmark is redirected and centralized to one warehouse in Denmark. In the third scenario, the total export flow of products from both Sweden and Denmark is instead redirected and centralized to one warehouse in Sweden. To evaluate and compare the different distribution structures a general model was first created by combining different theoretical models and adapting them to the context of overseas distribution for frozen food. The study then included the three phases of developing the model to fit the case company, applying the model on the case company and then to finally evaluating the model. The resulting model, which was the outcome of the development process, can be seen below. The model illustrates the different included elements. By then applying the model onto the case company, HKScan, it was found that a centralization to a joint warehouse in Denmark would make total cost savings of several percents. In addition, this scenario would increase the total service level. However, the environmental impact would be increased due to long cross-border road transport distances and longer land and sea transports from the warehouse. In addition, it was not possible to fully investigate whether such a distribution would be possible from a regulatory point of view.  A centralization in each country would have minor regulatory issues, it would lead to the smallest environmental impact and have a slight increase in service levels as well as a reduction for the total cost of one percent. The evaluation of the model showed that it produces reasonable results with the regulatory elements being the hardest to evaluate for the different scenarios. Regarding the detail level, the veterinary element could be accounted for by the warehousing element and the sea freight element split into transport from warehouse to domestic port and sea freight from domestic port to the destination port. The box-model, containing twelve elements, can be seen as generalizable for evaluating distribution structures in similar contexts, Overseas export of frozen food. However, the calculation performed within the model do probably only apply to the specific scenarios in the study.

Master of Science / Department of Clinical Sciences / Maria S. Ferrer / Presence of anti-sperm antibodies (ASA) is associated with infertility in many species, including bulls but there is no standardized direct technique that allows detection of ASA bound to the sperm surface. The overall objective was to standardize a flow cytometric technique for detection of IgG and IgA directly attached to bovine sperm. The effects of fixation using phosphate buffer solution (PBS) or diluted formalin buffer solution (dFBS), exclusion of dead cells from the analysis, and aliquot variability were assessed using healthy bulls classified as Satisfactory Potential Breeders (SPB, n=9) and bulls with experimentally induced ASA (n=4) (Experiment1). The effect of freezing on the percentage of IgG- and IgA- bound sperm was assessed in samples from immunized bulls (n=4) (Experiment 2). Anti-sperm antibodies on the sperm surface were induced in yearling bulls by intramuscular injection of autologous semen and an adjuvant. Fixation of sperm cells did not affect the percentage of IgG- or IgA-bound sperm in any group of bulls. Exclusion of dead cell from the analysis did not affect the percentage of IgG-bound sperm (p= 0.0922 and p= 0.1525 for immunized and reproductively normal bulls, respectively). The exclusion of dead cells significantly increased the percentage of IgA-bound sperm in semen samples from immunized bulls (p= 0.0152) and significantly decreased the percentage of IgA- bound sperm in semen samples from reproductively normal bulls (p= 0.0012). Variability was < 10% in samples from immunized and reproductively normal bulls for percentage of IgG- and IgA-bound sperm. Freezing did not affect the percentage of IgG- (p=0.1287) or IgA-bound sperm (p=0.4175). Based on these results, fixation is neither necessary nor detrimental for analysis, and the percentage of antibody-bound cell should be calculated gated on the population of live cells only, especially when evaluating IgA binding. The percentage of ASA-bound sperm can be assessed on frozen-thawed samples. The development of this technique allows for further studies on ASA-bound sperm in populations of normal and abnormal bulls.