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Data within an organization is highly structured and organized into specified applications or systems. These systems have a different function within an organization, so each user will have a different level to access each system. So by the data mapping approach user can easily isolate those data and prepare the declarations for the available data element. Generic Modular Mapping Framework (GEMMA) a new common generic framework for data mapping was developed by Airbus Group Innovation GmbH to avoid numerous potential issues in matching data from one source to another. It is geared towards the high flexibility in dealing with a large number of different challenges in handling huge data. It has an open architecture that allows the inclusion of the application-specific code and provides a generic rule-based mapping engine that allows the users to define their own mapping rules. But GEMMA tool is presently used to read and process the data on the fly in memory, as each time the tool is used for mapping data from different sources. This has an impact on large memory consumption when handling large data and is inefficient in storing and retrieving the session data which are the user decisions. This paper provides a detailed description of the GEMMA tool, with the new concept for specific requirements inherited in the framework and in the current architecture to achieve the goals.

Data within an organization is highly structured and organized into specified applications or systems. These systems have a different function within an organization, so each user will have a different level to access each system. So by the data mapping approach user can easily isolate those data and prepare the declarations for the available data element. Generic Modular Mapping Framework (GEMMA) a new common generic framework for data mapping was developed by Airbus Group Innovation GmbH to avoid numerous potential issues in matching data from one source to another. It is geared towards the high flexibility in dealing with a large number of different challenges in handling huge data. It has an open architecture that allows the inclusion of the application-specific code and provides a generic rule-based mapping engine that allows the users to define their own mapping rules. But GEMMA tool is presently used to read and process the data on the fly in memory, as each time the tool is used for mapping data from different sources. This has an impact on large memory consumption when handling large data and is inefficient in storing and retrieving the session data which are the user decisions. This paper provides a detailed description of the GEMMA tool, with the new concept for specific requirements inherited in the framework and in the current architecture to achieve the goals.

Ribonucleotide reductase (RNR) is a key enzyme in the de novo biosynthesis and homeostatic maintenance of all four DNA building blocks by being able to make deoxyribonucleotides from the corresponding ribonucleotides. It is important for the cell to control the production of a balanced supply of the dNTPs to minimize misincorporations in DNA. Because RNR is the rate-limiting enzyme in DNA synthesis, it is an important target for antimicrobial and antiproliferative molecules. The enzyme RNR has one of the most sophisticated allosteric regulations known in Nature with four allosteric effectors (ATP, dATP, dGTP, and dTTP) and two allosteric sites. One of the sites (s-site) controls the substrate specificity of the enzyme, whereas the other one (a-site) regulates the overall activity.  The a-site binds either dATP, which inhibits the enzyme or ATP that activates the enzyme. In eukaryotes, ATP activation is directly through the a-site and in E. coli it is a cross-talk effect between the a and s-sites. It is important to study and get more knowledge about the overall activity regulation of RNR, both because it has an important physiological function, but also because it may provide important clues to the design of antibacterial and antiproliferative drugs, which can target RNR. Previous studies of class I RNRs, the class found in nearly all eukaryotes and many prokaryotes have revealed that the overall activity regulation is dependent on the formation of oligomeric complexes. The class I RNR consists of two subunits, a large α subunit, and a small β subunit. The oligomeric complexes vary between different species with the mammalian and yeast enzymes cycle between structurally different active and inactive α6β2 complexes, and the E. coli enzyme cycles between active α2β2 and inactive α4β4 complexes. Because RNR equilibrates between many different oligomeric forms that are not resolved by most conventional methods, we have used a technique termed gas-phase electrophoretic macromolecule analysis (GEMMA). In the present studies, our focus is on characterizing both prokaryotic and mammalian class I RNRs. In one of our projects, we have studied the class I RNR from Pseudomonas aeruginosa and found that it represents a novel mechanism of overall activity allosteric regulation, which is different from the two known overall activity allosteric regulation found in E. coli and eukaryotic RNRs, respectively.  The structural differences between the bacterial and the eukaryote class I RNRs are interesting from a drug developmental viewpoint because they open up the possibility of finding inhibitors that selectively target the pathogens. The biochemical data that we have published in the above project was later supported by crystal structure and solution X-ray scattering data that we published together with Derek T. Logan`s research group. We have also studied the effect of a novel antiproliferative molecule, NSC73735, on the oligomerization of the human RNR large subunit. This collaborative research results showed that the molecule NSC73735 is the first reported non-nucleoside molecule which alters the oligomerization to inhibit human RNR and the molecule disrupts the cell cycle distribution in human leukemia cells.

Ce document résume mon activité de recherche depuis 1991. Elle a porté sur deux thématiques connexes : la modélisation et la validation de langages métier de l'automatisme et le contrôle à distance de systèmes mécaniques sur des réseaux de communication non fiables. Le premier axe de recherche portait plus précisément sur la définition formelle des langages classiques de l'automatisme (langage à relais et Grafcet) à l'aide des formalismes synchrones tels que Signal ou Lustre. L'intérêt d'un tel travail était de pouvoir exprimer les différentes interprétations possibles d'un même programme afin de pouvoir les comparer ; les environnements développés autour des formalismes synchrones permettant alors la réalisation directe de simulateurs ainsi que l'ouverture vers des outils autorisant la validation des programmes. Dans ce dernier domaine, différentes pistes ont été étudiées avant de retenir une modélisation à l'aide d'automates temporisés permettant alors l'expression de propriétés temporelles sur les programmes modélisés. Le deuxième axe de travail avait et a pour objectif d'étudier la faisabilité de l'introduction des nouvelles technologies, et en particulier des technologies réseau, dans le contrôle supervisé de systèmes mécaniques. Le réseau est ici vu comme un réseau sans qualité de service, de type Internet, alors que l'une des préoccupations majeures des automaticiens est d'assurer une sécurité maximale. Dans un premier temps, nous avons proposé une architecture logicielle permettant la communication entre l'opérateur et le système à contrôler. Basée sur un noyau de services complété par des pilotes spécifiques aux applications cibles, elle est facilement extensible. Pour prendre en compte les aléas réseau, la méthodologie Gemma, utilisée en ingéniérie des automatismes, a été étendue afin d'intégrer des informations en provenance d'un capteur réseau. Ce dernier permet d'estimer la qualité de la connexion d'un bout à l'autre du système de communication et doit être pris en compte automatiquement, et en permanence, par le système afin d'assurer la sécurité du contrôle supervisé. Bien que différentes, ces deux thématiques se rejoignent sur de nombreux points : --- Le domaine d'application, c'est à dire le contrôle de systèmes en environnement de production industriel. D'un côté, on a cherché à assurer une meilleure qualité, de la spécification à l'implantation en passant par la vérification des applications. De l'autre en proposant l'utilisation des nouvelles technologies de communication pour améliorer la productivité globale de l'entreprise. --- L'utilisation et l'introduction des méthodes et des outils de l'informaticien auprès de la communauté des automaticiens. Dans mon premier domaine de recherche, par l'intermédiaire du monde dit "synchrone" et dans le deuxième par la mise en oeuvre des technologies autour de l'Internet. Dans les deux cas, les technologies informatiques se sont placées au service d'autres disciplines tout en essayant de faire ressortir les points difficiles et de proposer des solutions innovantes. --- Le souci permanent de construire des applications sûres et de valider, si possible de manière formelle, les résultats obtenus. Cet aspect est surtout visible dans le cadre de la première activité de recherche, mais des travaux, non encore achevés, ont également porté sur la validation de l'architecture logicielle proposée pour le contrôle à distance. Ils sont directement issus des connaissances et de l'expérience acquises dans le premier thème.

Background.The extracellular matrix surrounds all cells within our bodies. The glycosaminoglycan hyaluronan is a major component in the extracellular matrix. Despite its structural simplicity it has been shown to be involved in several important functions. It is a lubricant and shock absorber, as well as an important player in inflammation and tumor invasion. Many of its functions are closely related to its size and concentration in tissues. Therefore methods for measuring these properties are of great importance to properly understand the role that hyaluronan play in different events. Proteins are found both inside and outside cells, and they have a wide variety of functions. The protein structure and function is determined by the properties of their building blocks, the amino acids. Several diseases have been linked to changes in the amino acid sequence of certain proteins by mutations, causing the proteins to form extracellular deposits of structures called amyloid aggregates. The aim of this thesis is to investigate the function of hyaluronan in cell cultures, develop new methods for size determination hyaluronan and to use multivariate methods to provide prediction and better understanding of factors driving protein amyloid aggregation. Methods.Cardiomyocytes and fibroblast were cultured and stimulated by different growth factors. Hyaluronan was purified and its size and concentration were measured. Crosstalk between cardiomyocytes and fibroblast were investigated and gene expression of hyaluronan synthases was determined. A new method for size measurement of hyaluronan was developed. The amyloid aggregation rate of different mutants of acylphosphatase was predicted by multivariate analysis. Results. Cardiomyocytes stimulated by PDGF-BB produced hyaluronan. Cardiomyocytes could induce fibroblast to increase its hyaluronan production, through an unknown soluble factor. The cardiomyocyte gene expression changed when stimulated by hyaluronan. GEMMA was presented as a new method for size determination of hyaluronan. Amyloid aggregation of different acylphosphatase mutants could be predicted using a multivariate regression model of the physicochemical and structural properties of the amino acid sequence. Conclusion. It was shown that cardiomyocytes are not only able to produce hyaluronan, but also induce an increased hyaluronan production in other cells. GEMMA was proven suitable for size determination of hyaluronan at very low concentrations. Multivariate analysis showed that hydrophobic patterns and charge where the most important factors for amyloid aggregation of acylphosphatase.

Made available in DSpace on 2015-03-03T11:52:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-06-23Bitstream added on 2015-03-03T12:07:19Z : No. of bitstreams: 1 000810727.pdf: 2628519 bytes, checksum: 12d95db5dfa9b2c40e24fa59747265be (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Os estudos sobre a literatura de viagem desenvolveram-se a partir da década de 1980 paralelamente ao desenvolvimento dos estudos pós-coloniais. Antes desse período era vista como um subgênero ou um gênero menor relacionado com a autobiografia. Em parte, isso ocorreu pela dificuldade de se formular uma definição que auxiliasse em sua classificação dentro dos padrões tradicionais, mas também porque a literatura de viagem comporta textos de diversas formas (diários, relatórios, cartas,etc.) produzidos por viajantes de diferentes origens, homens e mulheres. No caso das mulheres, esses textos são em número menor porque, devido a questões históricas e culturais, a viagem feminina era mais difícil. Alguns teóricos apresentados nesta tese defendem a ideia de que muitos livros de viagem são o resultado de um processo de seleção e de uma elaboração narrativa no qual o viajante cria sua própria versão da realidade ficando no limite entre a veracidade e a ficção. Baseando-nos nessa premissa, esta tese analisa como se dá esse processo na obra Nostra Signora del Mar Dolce (Missioni e Paesaggi di Amazzonia) da escritora e jornalista italiana Gemma Ferruggia (1867-1930), publicado em 1902, relato de uma viagem ao Pará e ao Amazonas realizada em 1898 em companhia do marido, Alberto Manzi. Nosso objetivo é identificar as estratégias da autora para (re)criar sua experiência de viagem através da escrita considerando alguns elementos, tais como sua experiência como ficcionista, as leituras de outros viajantes e de obras brasileiras e a questão do gênero / Studies on travel literature evolved from the 1980s with the development of post-colonial studies, before this period was seen as a minor genre or subgenre associated with the autobiography. In part, this was due to the difficulty of formulating a definition that would help in their classification within the traditional patterns, but also because the travel literature comprises texts in different forms (diaries, reports, letters, etc). produced by male and female travelers from different origins. For women, these texts are fewer because, due to historical and cultural issues, women's journey was more difficult. Some theorists presented in this study support the idea that many travel books are the result of a process of selection and preparation of a narrative in which the traveler creates their own version of reality getting in the boundary between truth and fiction. Based on this premise, this thesis analyzes how this process occurs in the work Nostra Signora del Mar Dolce (Missioni e Paesaggi di Amazzonia), by the Italian writer and journalist Gemma Ferruggia (1867-1930), published in 1902, in which she tells about her journey to Pará and Amazonas held in 1898 with her husband, Alberto Manzi. Our aim is to identify the author’s strategies to (re)create her travel experience through writing by considering some elements, such as her experience as a fiction writer, her readings from other travelers and Brazilian works and gender issues

This thesis examines the ways in which the Magna Mater became an integral part of Augustan ideology and the visual language of the early principate. Traditionally, our picture of the Augustan Magna Mater has been shaped by evidence from literary sources. Here, however, the monuments of the goddess' cult are considered in their religio-political context. Works that link Augustus himself to the Magna Mater are shown to reveal that the goddess played a significant and hitherto unappreciated role in official propaganda. Part I examines the nature of the Augustan reconstruction of the Palatine Temple of the Magna Mater and challenges persistent claims that the princeps was disinterested in the metroac cult. Augustus' use of inexpensive building materials is shown to be, not a display of parsimony, but an attempt to retain the traditional appearance of a venerable structure. A reinterpretation of the temple's pedimental and acroterial sculpture, using the Valle-Medici reliefs, demonstrates that Augustus promoted the Magna Mater as an allegory of Rome's Trojan heritage and as a symbol of a new Golden Age. Part II investigates the topography of the Augustan precinct on the Palatine, and argues that the geographic linkage of the metroön and the House of Augustus became a topos in imperial imagery. It then demonstrates that several well-known works of art echo this connection between the princeps and the goddess. These works range from statues in the Circus Maximus designed to be viewed by thousands, to the Gemma Augustea, a luxury item intended for the elite. They are also found both inside and outside Rome. A reassessment of the Vicus Sandaliarius altar and the Sorrento base illustrates popular recognition of Augustus' reinvention of the Magna Mater as a national deity of Rome and the tutelary goddess of the Julio-Claudii.

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