Les prairies semi-naturelles constituent des espaces multifonctionnels susceptibles de rendre de nombreux services à la société. Ce sont en particulier des agro-écosystèmes favorables à l’expression d’une grande diversité floristique et faunistique permettant de concilier enjeux écologiques et agronomiques. Ce travail de thèse vise à analyser (i) l'influence des facteurs environnementaux sur l'assemblage des communautés in situ et (ii) les relations entre la structure fonctionnelle des assemblages (i.e. valeurs de traits agrégées et diversité fonctionnelle) et leurs performances (i.e. production de biomasse et digestibilité de cette biomasse). Cette étude s'appuie sur des approches expérimentales et de terrain dans les prairies humides pâturées du Marais Poitevin. Nos résultats montrent des réponses très différentes des traits aériens et racinaires étudiés aux gradients de stress et de perturbation rencontrés dans ces prairies. Ils ont également permis de mettre en évidence l'influence prépondérante des valeurs de traits agrégées sur la fourniture de services écosystémiques (i.e. productivité et digestibilité du fourrage), en accord avec la "Biomass ratio hypothesis" énoncée par Grime. L'influence du niveau de diversité fonctionnelle sur les services rendus par les assemblages varie selon le service considéré. Contrairement aux résultats attendus selon la "Diversity hypothesis" énoncée par Tilman, le niveau de diversité fonctionnelle est lié négativement à la productivité des assemblages. En revanche, un effet positif de la diversité fonctionnelle sur la digestibilité a été mis en évidence. Cette étude a par ailleurs permis de montrer un compromis entre la productivité des assemblages et la digestibilité de la biomasse produite dans ces prairies humides. Ce travail suggère que des outils de diagnostics de la valeur fourragère du couvert peuvent être construits sur la base de la structure fonctionnelle des assemblages, et ce avec un haut niveau de fiabilité. Il met également en avant les bénéfices agronomiques associés à l'hétérogénéité des couverts végétaux rencontrés dans ces prairies. / Semi-natural grasslands form multifunctional areas susceptible to serve the society from many aspects. Indeed, they are agro-ecosystems that promote expression of a large floristic and faunistic diversity, which allows conciliating ecologic and agronomic issues. This PhD work aims at analysing (i) influence of environmental factors on natural communities' assembly rules and (ii) the relationships between the functional structure of assemblages (i.e. community weighted mean trait values and functional diversity) and their performances (i.e. biomass production and its digestibility). This study is based on both experimental and field approaches in the Marais Poitevin wet grasslands. Our results show that the studied aerial and root traits display a wide diversity of answers to stress gradients and disturbances encountered in these grasslands. Our results also highlighted the crucial influence of community weighted mean trait values on provision of ecosystem services (i.e. forage productivity and digestibility), in agreement with the Biomass ratio hypothesis set out by Grime. The influence of the functional diversity level on services provided by the assemblages differs depending on the service considered. Contrary to the results expected according to the Diversity hypothesis of Tilman, the functional diversity level is negatively correlated with the productivity of the assemblages. However, our study points to a positive feedback between functional diversity and digestibility. Furthermore, this study demonstrated the existence of trade-off between the productivity of assemblages and the digestibility of biomass produced in these wet grasslands. This work suggests that highly reliable tools can be established, based on the functional structure of assemblages, to evaluate the feeding value of a vegetal cover. This work finally illustrates the agronomic benefits associated with heterogeneous vegetal covers encountered in these grasslands.

Though some researchers have argued that the Big Barrens grasslands of Kentucky were the product of anthropogenic land clearing practices by Native Americans, heretofore, this hypothesis had not been tested archaeologically. More work was needed to refine chronologies of fire activity in the region, determine the extent to which humans played a role in the process, and integrate these findings with the paleoenvironmental and archaeological record. With these goals in mind, I conducted archaeological and geoarchaeological investigations at Crumps Sink in the Sinkhole Plain of Kentucky. The archaeological record and site formation history of Crumps Sink were compared with environmental and archaeological data from the Interior Low Plateaus and Southern Appalachian Mountains for an understanding of how the site fits into the larger story of human-environmental interactions in the Eastern Woodlands. Based on the data recovered, I argue that through land burning Archaic hunter-gatherers were active managers of ecosystems to a greater degree than previously acknowledged. Excavations at Crumps Sink revealed stratified archaeological deposits spanning the late Middle Archaic to Terminal Late Archaic periods. Radiocarbon dates and an analysis of projectile point typologies provided information on the chronological and cultural history of the site. Magnetic susceptibility, loss-on-ignition, plant available phosphorous, and soil micromorphological analyses were conducted to examine landform dynamics in response to environmental change and to trace the anthropogenic signature created by human activities at the site. Masses of lithic debitage, animal bone, and burned sediment nodules per ten-cm-level provide an indication of human occupation intensity and shifting activities over time. Radiocarbon dates were used to reconstruct rates of sediment accumulation in the sink. These varying datasets were considered together for a holistic understanding of localized environmental and anthropogenic impacts on the landform. Between 7200 and 5600 cal. BP, during the Middle Holocene Thermal Maximum and corresponding with the late Middle Archaic period, sediment accumulation was sustained with one identifiable episode of very weak soil development. Background magnetic and chemical signatures in the soils were greater than they were at pre-occupation levels, demonstrating that human activities left a lasting imprint in soils as early as the late Middle Archaic period. Between 5600 and 3900 cal. BP, periods of diminished sedimentation led to more pronounced episodes of soil formation. However, these soil horizons are interposed by pulses of enhanced sediment accumulation. These soil data may signal shifting environmental regimes during the Middle to Late Holocene transition. Between 5600 and 3900 cal. BP scattered plant ash, elevated masses of burned sediment nodules, and pestle fragments in Late Archaic deposits suggest that hunter-gatherers were intensively processing nut mast, potentially in association with early forest clearance and silviculture. Botanical assemblages from a coincident archaeological sequence at the Carlston Annis site in the nearby middle Green River region has demonstrated woodland disturbance and potential silviculture in central Kentucky during this time. During the Late Archaic and Terminal Late Archaic periods (3900-3000 cal. BP), substantial plant ash deposition occurred in a stratum that accumulated relatively quickly. Very low burned sediment nodule masses in this deposit indicate that combustion features were not common in the immediate vicinity and that elevated frequencies of plant ash were the result of burning on a broader expanse of the surrounding landform. Chronologically, the zone with enhanced plant ash deposition is coeval with previously demonstrated occurrences of increased forest fires, grassland expansion, and a shift to early horticultural economies throughout the region. Soil development occurred after 3000 cal. BP, and this episode of landform stability may have lasted for over two millennia until being capped by sediment accumulation from historic agriculture. The late Middle Archaic through Terminal Late Archaic data from Crumps Sink demonstrate that hunter-gatherer activities left lasting signatures in soils in Kentucky. The data from the Late Archaic to Terminal Late Archaic periods (ca. 5600-3000 cal. BP) may indicate intentional land burning by hunter-gatherers to create anthropogenic environments, first for silviculture and then for early plant domestication. This forces a rethinking of labor and subsistence systems within hunter-gatherer societies. Thus, if hunter-gatherers were utilizing long-term forest management methods, they were employing a delayed-return economic system relying on labor investment and negotiated understandings about land tenure. Further characterization of the origin of fire management activities will help us to elucidate the nature of incipient indigenous plant domestication in the Eastern Woodlands.

In this thesis, I examine the variation in plant species richness along gradients of productivity and disturbance in grasslands and forest habitats in southern Sweden, and I compare the documented patterns with theoretical predictions. Moreover, I evaluate the relative importance of habitat quality and habitat configuration for the occurrence of field layer species in deciduous forests. Finally, I present a new method for the determination of the regional species pool. To examine regional and local variation in plant species richness, I gathered data on species composition in plots of different size (0.001 - 1000 m2) in three vegetation types (deciduous forests, dry grasslands and coastal meadows) in four regions of southern Sweden (Öland, Gotland, Småland and Uppland). As predicted by the species pool hypothesis, differences in small-scale species richness of deciduous forests and dry grasslands were correlated with differences in the size of the regional species pool. Moreover, among plots large-scale diversity was predictive of small-scale diversity. Species diversity showed a hump-shaped relationship with productivity in forests, and was related to environmental heterogeneity and the size of the 'habitat-specific' species pool. In the two types of grassland examined, grazed sites were richer in species than abandoned sites. Moreover, both species composition and the representation of plants with different life-history characteristics differed between grazed and abandoned sites. As predicted by the intermediate disturbance hypothesis, species richness was highest at intermediate levels of grazing in coastal meadows. However, all the above patterns were scale-dependent, and not observed at all plot sizes. The occurrence of field layer species in deciduous forests was more strongly related to habitat quality (mainly soil factors) than to habitat configuration (forest area and isolation). Across species, low seed production, clonal reproduction and habitat specificity were negatively associated with isolation.

<p>In the winter of 1819 the United States shook under the first Great Depression, and on the Missouri River a great military/scientific enterprise sent to secure Missouri Territory shivered and died from cholera and scurvy. In 1820 Maj. Stephen Long and a poorly equipped expedition of twenty-three soldiers, amateur scientists, and landscape painters, set out from Engineer Cantonment to circumnavigate the unknown Central Great Plains during the height of summer, and rescue something from the debacle. After weathering endless rain and hallucinating waves of Comanche, they divided into two groups at the Arkansas, and then either starved and endured weeks of rain on the lower Arkansas, or ate rancid skunk and endured blistering sun on the 'Red River'. On return they found Long had 'mistaken' the Canadian River for the Red, and that they were yet another failed expedition to know the Louisiana Purchase. Unsurprisingly, Long labeled the whole place a "great desert." An editor improved the phrase to <i>Great American Desert</i>, and emblazoned the phrase on history.</p> <p><i>A Persistent Mirage</i> is both an exegesis of the GAD myth and an HGIS study of the groups and biomes the desert mirage occludes. Desert was a cultural term meaning <i>beyond the pale</i> that beached with the Puritans. Like Turner's frontier, it stayed a step ahead of settlement, moving west to the tall grass prairies before crossing the Mississippi to colonize the Great Plains. Once there it did calculable damage to the writing of Plains Aboriginal history. After all, who lives upon deserts but wandering beasts and savages? Beneath the mirage was an aboriginal network of agricardos, or agricultural and trading centers, growing enough food to support large populations, and produce tradable surpluses, under-girded by bison protein. Euramericans from Cabeza de Vaca on were drawn to agricardos which helped broker the passages of horses to the Northern Plains and of firearms to the Southwest. While some withstood epidemic disease, the escalation of inter-group violence and environmental degradation due to the adoption of the horse by agricardo groups proved their undoing. Beneath the Great American Desert lies the great Indian agricardo complex, with its history just begun.</p>

<p>In the winter of 1819 the United States shook under the first Great Depression, and on the Missouri River a great military/scientific enterprise sent to secure Missouri Territory shivered and died from cholera and scurvy. In 1820 Maj. Stephen Long and a poorly equipped expedition of twenty-three soldiers, amateur scientists, and landscape painters, set out from Engineer Cantonment to circumnavigate the unknown Central Great Plains during the height of summer, and rescue something from the debacle. After weathering endless rain and hallucinating waves of Comanche, they divided into two groups at the Arkansas, and then either starved and endured weeks of rain on the lower Arkansas, or ate rancid skunk and endured blistering sun on the 'Red River'. On return they found Long had 'mistaken' the Canadian River for the Red, and that they were yet another failed expedition to know the Louisiana Purchase. Unsurprisingly, Long labeled the whole place a "great desert." An editor improved the phrase to <i>Great American Desert</i>, and emblazoned the phrase on history.</p> <p><i>A Persistent Mirage</i> is both an exegesis of the GAD myth and an HGIS study of the groups and biomes the desert mirage occludes. Desert was a cultural term meaning <i>beyond the pale</i> that beached with the Puritans. Like Turner's frontier, it stayed a step ahead of settlement, moving west to the tall grass prairies before crossing the Mississippi to colonize the Great Plains. Once there it did calculable damage to the writing of Plains Aboriginal history. After all, who lives upon deserts but wandering beasts and savages? Beneath the mirage was an aboriginal network of agricardos, or agricultural and trading centers, growing enough food to support large populations, and produce tradable surpluses, under-girded by bison protein. Euramericans from Cabeza de Vaca on were drawn to agricardos which helped broker the passages of horses to the Northern Plains and of firearms to the Southwest. While some withstood epidemic disease, the escalation of inter-group violence and environmental degradation due to the adoption of the horse by agricardo groups proved their undoing. Beneath the Great American Desert lies the great Indian agricardo complex, with its history just begun.</p>

M. Tech. Environmental Management. / Aims to investigate the impacts of Montane grassland management practices, particularly through grazing intensity and burning frequency, on ground living spider assemblage structure.More specific aims include:to note any relationship between particular spider species to specific forms of habitat management ; to note the loss of particular species at detected thresholds in management practices ; and to model spider assemblage structure based on grassland management.

The South African grassland biome is disappearing rapidly through advancing development and change in agricultural land use. One of the most threatened grassland types, Midlands Mistbelt, in the KwaZulu-Natal Midlands is an extremely diverse and home to many endemic species across an array of taxa. Three taxa, namely, grasses, grasshoppers and butterflies represent various trophic levels, which are important to the functioning of the grasslands. Ten grasslands were sampled by walking ten fifty metre transects for a twelve-month period. The grasslands were selected as they represented a range of management practices and varying environmental conditions. Using Indicator Species Analysis (ISA) twenty-two species of grasshopper were identified as indicators of environmental variables and management practices. The abundances of the various species indicated the intensity of the management regimes or disturbances. Using the twenty-two grasshopper species abundances and a three hundred point sampling assessment of the grasses creates an assessment tool that can rapidly appraise the management of the grassland, but due to lack of data for other taxa, cannot assess whether management practices for the focal taxa create congruent results for non-focal taxa. Two of the three taxa proved to be good indicators of grassland health, whilst the third, butterflies were ineffectual, due to low abundance and richness. From the results it was concluded that burning was taking place to frequently, and required a reduction to every four years, as this would improve butterfly richness and abundance, and increase abundance of endemic and flightless grasshopper speCies. A rotational grazing system needs to be implemented at sites where continual grazing takes place, wildlife or livestock, impacts on the grassland condition and species diversity. Increasing habitat heterogeneity increases species diversity, and allows later successional species to be included in the grasshopper assemblage. Management of the grasslands in the KwaZulu-Natal Midlands needs to be more responsive and adaptive. In addition, small fragment management needs to be intensified to provide a range of habitats and refugia that will suit all species. This study advocates the use of grasshoppers and grasses as suitable biotic indicators of grasslands in the KwaZuluNatal Midlands. / Thesis (Ph.D.) - University of KwaZulu-Natal, Pietermaritzburg, 2005

The overall aim of the study was to investigate re-vegetation of disturbed sites, using nursery grown plugs (from seedling trays) of Themeda triandra, Heteropogon contortus and Hyparrhenia dregeana in order to reach practical management guidelines for re-vegetation using indigenous grass plugs. A number of field trials were set up at Kamberg Nature Reserve (29°24'S, 29°40'E) on a site that was clear felled of A. mearnsii in October 1997. The trials were established in January 1998 and January 1999. A total of approximately 52 ,000 nursery raised plugs of T. triandra, H. contortus and H. dregeana were planted into an area of approximately 7,000 m2 . In the planting density trial , plugs of H. dregeana only and a combination of T. triandra/H. contortus were planted at 15 cm and 30 cm spacings. The T. triandra/H. contortus combination at 30 spacing showed the greatest survival and lateral plant growth (tiller number and basal area) and this combination is thus recommended. In the over-sowing trials, the H. dregeana and T. triandra/H. contortus combination at both 15 cm and 30 cm spacing were over-sown with E. curvula. The survival and lateral growth of the T. triandra/ H. contortus combination at 30 cm was again greater than the other treatments. Over-sowing with E. curvula suppressed the survival and lateral growth of the planted plugs across all treatments compared to not over-sowing. The over-sown conditions showed a significant decrease in the diversity of the plots, both in the number of species present and the Shannon diversity index. An area that had been cleared of A. mearnsii and sown to E. curvula 25 years previously was shown to have a lower number of species than the neighbouring veld. Nursery raised plugs of T. triandra were planted into the mature E. curvula in an attempt to improve the biodiversity of these areas. To re-introduce T. triandra into these E. curvula swards the plugs must be planted into the centre of a gap rather than around the base of an E. curvula plant. For improved survival of the plugs the E. curvula tufts must be clipped, while for best lateral growth the E. curvula tufts must be sprayed with a glyphosate herbicide three months prior to planting and clipping. However, the added expense of spraying and clipping is not warranted as the clipped treatments also showed good growth. Transplant shock is common when planting nursery raised plugs out into the field, as there is a relatively small root volume in the plug compared to the above ground leaf biomass. Alleviation of moisture stress at planting using a starch based polymer with high water holding capacity (Terrasorb®) and a white, needle punched geo-fabric (Agrilen®) to provide a seven day period of artificial shade after planting did not show significant improvements over the control with regards to survival or plant growth. Thus these methods of moisture amelioration are not recommended in revegetation through planting of plugs at this study site. A trial was established to investigate the biomass production of six different treatments to determine their potential to support a fire. The total biomass for the plots which were over-sown by E. tef and planted to only H. dregeana were on average sufficient for a fire, but there was a discontinuous fuel load across these plots, especially in the replications that had very low survival rates and thus these plots could not be burnt. The control and herbicide sprayed plots also showed sufficient fuel load for a fire, but this fuel load was made up of A. mearnsii saplings and bramble with very little grass cover and thus a fire would not have burnt through these plots either. The T. triandra/H. contortus combination did not produce sufficient fuel load, due to poor survival. Thus only the plots over-sown with E. curvula were able to burn in this trial and as a burning trial per se the trial was abandoned. Seed bearing hay (thatch) was collected in early summer (December 1997) and late summer (April 1998). Both times of year of harvesting proved to be successful in terms of grass cover, although the early harvested thatch had a greater number of species per plot. The Shannon diversity indexes of the two treatments were not significantly different. The multi-response permutation procedure technique confirmed that there was a compositional difference between the treatments. By the end of the trial Harpochloa falx and T. triandra and H. dregeana were indicators for the early and the late harvested thatch respectively. Comparing the thatching trial and the planting density trial indicated that the T. triandra/H. contortus combination at 30 cm spacing would be recommended to maximize biodiversity. The summer months have been shown to be the best time to plant the plugs, although the actual success will be dependant on the conditions within a particular year. The plugs should not be kept in the nursery for longer than three months and larger plugs (96 seedlings per tray) should be used. Nursery raised plugs of T. triandra and H. contortus were planted in an equal mix in an area that was cleared of A. mearnsii in 1996. By June 1998 661 H. contortus seedlings and 14 T. triandra seedlings had germinated naturally. The November 1998 population consisted of 418 H. contortus seedlings and 18 T. triandra seedlings. By May 2000 the June 1998 population showed a survival of 78.4% and the November 1998 population showed a survival of 91 .1 %. In the various trials, the ability of the nursery raised plugs used for re-vegetation to suppress the regrowth of A. mearnsii was investigated by determining the number of A. mearnsii seedlings per metre squared. The plant spacing and species of plugs used did not have a significant effect on the number of A. mearnsii seedlings per metre squared. Over-sowing with E. curvula did, however, significantly suppress the wattle re-growth. In the thatching trial the early harvested plots showed lower numbers of A. mearnsii per metre squared than the late harvest plots, as they were covered with a thick layer of thatch soon after the A. mearnsii was cleared which suppressed the A. mearnsii re-growth. Although E. curvula is able to produce a high biomass and suppress the A. meansii seedlings, it has a detrimental effect on the biodiversity of the area. Therefore, in conservation areas, where biodiversity is of great importance the planted plugs (at 30 cm spacing) or seed bearing hay must be used in preference to sowing E. curvula , although it must be remembered that greater follow up control is likely to be needed with planted plugs or seed bearing hay. The area must be planted or thatched as soon as possible after clear felling to provide competition for the A. mearnsii seedlings. / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2005.

The impounding of the waters of the Pongolo river, upstream of its floodplain on the Mocambique coastal plain, may adversely affect the functioning of the floodplain system. A multidisciplinary study of the functioning of the floodplain was initiated to provide a basis for the development of a management strategy for the floodplain. The study reported in this dissertation considered the flood dependence and functioning of the vegetation of the seasonally inundated area. The vegetation was mapped and the communities ordinated, according to the Braun-Blanquet technique, in relation to their positions relative to high flood level (HFL) and the level of the water after flood subsidence (i.e. maximum retention level, MRL). Community distribution was shown to be strictly determined by both the height of the floods and by the MRL. It was concluded that periodic floods are essential for the maintenance of the communities. The Cynodon dactylon (L.) Pers. Community, which forms extensive meadows in the zone of periodic inundation, was studied in detail. As the water level receded during winter, productivity was high (up to 23 kg ha¯¹ d¯¹1 dry mass) and a palatable sward was produced. This is heavily grazed by domestic stock, but as the soil dries out and water stress becomes significant, production decreases, C. dactylon becomes less palatable, and grazing shifts to newly exposed areas. The shift in grazing allows the build up of a large standing crop of both grazeable and ungrazeable (below ground and stolons) material. At the time of inundation by the next floods c. 910 kg ha¯¹ of dry mass, c. 17 kg ha¯¹ nitrogen and c. 2 kg ha¯¹ phosphorus have been removed by grazers. It is concluded that this production, which is flood dependent, forms an important supplement to stock grazing during winter. It is suggested that this source of grazing could be stimulated by irrigation during winter. Cynodon dactylon is shown to decompose rapidly during inundation, losing half of its mass and nutrients in c . 28 days. It therefore represents a major energy and nutrient input during the aquatic phase. The extent to which it is grazed during submergence is unknown. The nutrient input is derived ultimately from the soils of the inundated areas and, since nutrients are being removed by both terrestrial grazers and flushing, continued production is reliant upon the annual sediment load reaching the floodplain. Most of the sediment load will now be deposited in the impoundment, and fertilization may be necessary to maintain productivity. The response of C. dactylon to the seasonal fluctuations in water level are used to formulate proposals for water release from the dam. These include proposals for the short-term, i.e. until the demand for irrigation water conflicts with the requirements of the floodplain, and for the long-term, when less water will be available for the floodplain / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 1981.

Fire, natural or of anthropologic origin is a recurrent phenomenon in South African mesic grasslands. The species composition of these grasslands is sensitive to fire frequency and there exists a permanent fire-species relationship syndrome. The shift follows a general trend where, in the absence of fire for longer periods, the native grass species, possibly the most economically important grass species, give space to relatively less desirable and invasive species accompanied by significant decline in basal cover. Though much is known as to how the floristic composition of the grassland changes in response to fire, the underlying mechanisms responsible for changes in plant vigour and species composition are not fully understood. There exists limited information on germination requirements of the local flora and how recruitment of the species is influenced by fire, fire-related factors and the interaction of these factors among themselves is virtually unknown. Since the discovery by DE LANGE and BOUCHER (1990), plant-derived smoke is being widely studied as an important germination cue and the theme has been the subject of intense experimental research and theoretical work. Germination studies conducted on South African Fynbos, Californian Chaparral, and Australian species have illustrated the widespread ability of plant-derived smoke in promoting germination of species from both fire and non-fire prone habitats. Studies have also showed that plant-derived smoke plays a significant role in vegetative growth of many plants including horticultural and agricultural crops, though mechanisms of smoke action in enhancing germination and promoting plant growth are still under active research. This study sought to gain insight into the role of fire and fire-related cues and other related factors on germination and seedling growth of key grass species from fire-prone grassland in South Africa. In this study, various investigations were conducted on different aspects related to smoke-induced seed germination. Furthermore, in depth examinations were conducted on the effects of fire-related factors such as smoke, heat, soil and nutrients on seed germination and seedling growth of representative key mesic grassland species from South Africa. Laboratory-based germination and vigour experiments were conducted using smoke solutions. The aim was to examine if the strong fire-species relationship syndrome we encounter in post-fire mesic grassland in South Africa is related to the effect of plant-derived smoke on germination and seedling growth. In addition, the interaction of plant-derived smoke solutions with temperature was examined by incubating seeds at a range of temperatures. Treating seeds with smoke-water and butenolide, the germination rate (GR) and final germination percentage (FGP) were greater in 3 of the 6 species. Themeda triandra Forssk. and Tristachya leucothrix Trin. ex Nees showed the greatest response, with final germination increased from 43% to 67% and 35% to 63% respectively. These smoke solutions have also significantly enhanced germination in Eragrostis tef (Zucc.) Trotter from 62% to 80%. The degree of responsiveness varied from species to species and across different temperatures. Findings from this study suggested that plant-derived smoke and its interaction with temperature significantly influence the germination and seedling growth of the South African mesic grassland species, which can further impact on the grassland composition. To characterise the role of plant-derived smoke on certain economically important seed traits, the effect of smoke-water and a smoke-isolated butenolide on seed germination and seedling growth of Eragrostis tef (grass species which has moderately good tolerance to east African drought) was investigated at a range of temperatures, light conditions and osmotic potentials. Smoke-water (1:500 v/v) and butenolide (10ˉ⁸ M) treatment of the seeds increased percentage germination, seedling vigour and imbibition from high to low osmotic potential. These results suggested that smoke solutions have characteristics with overriding effects toppling stresses exerted from high temperature and low osmotic potential, thereby widening the temperature and moisture zone over which germination can occur. Furthermore, these findings suggested that the mode of smoke action in promoting seed germination is likely to be linked to the role of smoke in facilitating water uptake by seeds (imbibition). A simple and rapid bioassay was implemented to detect the germination activity of extracts from soils in pre/post-burn status. Soil samples taken from burnt, unburnt and adjacent plots at depths of 0-2, 2-4, 4-6 and 6-8 cm before and after burning mesic grassland in South Africa were analysed for germination activity over an eight-week period. Soil samples were extracted using dichloromethane and bioassayed using Grand Rapids lettuce (Lactuca sativa L.) achenes. The Grand Rapids lettuce seeds exhibited several-fold greater germination percentages when treated with extracts from burnt soil compared to the other plots. The magnitude of such an activity declined through time since burn. The Grand Rapids lettuce seeds also showed significantly greater germination percentage when treated with unburnt soil extracts compared to the control (distilled water) which indicates the existence of other factors controlling germination in unburnt soil. Germination percentage was negatively influenced in the adjacent plots which reflected the inhibitory role of smoke on adjacent plots neighbouring the burn. Findings from this study suggested that the germination activity of a burning plant-material (smoke) indeed diffuses into the soil and its persistence declines with time. Furthermore, the findings implied that fire and smoke may significantly influence the germination of the soil seed bank of mesic grassland in South Africa. The effect of smoke solutions of various concentrations and their interaction with soil macronutrients NPK in influencing seedling growth of three selected grass species namely, Eragrostis curvula, Panicum maximum and Themeda triandra was investigated. These grass species were selected on the basis of the contrasting response they previously showed to smoke treatments. Results showed that the interaction between species, treatment and nutrients for seedling vigour index was highly significant (P < 0.001). In the absence of NPK elements, the smoke solutions generally enhanced seedling growth in Themeda triandra, while suppressing seedling vigour indices of Eragrostis curvula and Panicum maximum. It is ecologically significant that not only smoke but also its interaction with soil macronutrients appears to be important in structuring the post-fire regeneration and colonisation processes. To examine the effect of fire-associated cues of smoke and high temperature on germination and seedling emergence from the mesic grassland soil seed bank in South Africa, and disentangle responsible factors, the effect of heat and smoke-water on seedling emergence and the resultant effect on biomass production was examined in a greenhouse tray experiment over 90 days. Soil seed bank samples obtained from 0-5 cm top soil were treated with smoke-water solution (SW 1:500 v/v), heat (H = 100 °C for 15 min) and heat + smoke-water (H = 100 °C for 15 min + SW = 1:500 v/v) combined. Significantly, the highest number of seedlings emerged from soil seed bank samples treated with heat + smoke-water combined followed by smoke-water alone. Heat + smoke-water treatment of soil seed bank samples also significantly enhanced the rate of seedling emergence and total dry biomass production. Heat treatment by itself was not statistically different from the control in many respects. Generally, these results suggest that both heat and smoke are important germination cues and play a curial role on germination and seedling emergence from the mesic grassland soil seed bank. A greater number of fire-loving Themeda triandra seedlings emerged from soils treated with smoke, though this was not statistically significant. Seedling emergence indicators showed that seedling emergence was favoured more by smoke and smoke + heat treatments compared to the control. Seedling growth indicators also showed that soil samples treated with smoke or smoke + heat combined produced significantly higher biomass than untreated samples. In general, findings from this comprehensive study suggest that plant-derived smoke and its interaction with temperature (heat) and soil nutrients significantly influences seed germination and seedling growth of South African mesic grassland species differently, this can further influence the grassland composition. The association of certain fire-climax species with the re-occurrence of fire can partly be explained due to the association of such species with fire-induced changes in the habitat. Post-fire conditions of low nutrient (NPK) availability, presence of high heat and smoke cues in the habitat may favour the competitive ability of native short and smoke-responsive species such as Themeda triandra and Tristachya leucothrix over those characteristic species of fertile habitats such as Eragrostis curvula and other non-smoke-responsive species. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.