In festen azobenzenhaltigen Polymeren wurde bei Bestrahlung mit blauem Licht ein makroskopischer Materialtransport beobachtet. Um die Dynamik der Gitterentstehung zu verfolgen, wurde am Speicherring für Synchrotronstrahlung ein Gitterschreibaufbau errichtet. Damit konnte erstmals in dieser Arbeit die Gitterbildungsgeschwindigkeit in-situ simultan mit Röntgen- und Lichtstreuung untersucht werden. Mit Hilfe einer speziellen Anpassung der Röntgenstreutheorie konnten sehr gute Übereinstimmungen von theoretischen Berechnungen mit den Messergebnissen erzielt werden. Dabei konnte nachgewiesen werden, dass sich zeitgleich mit einem Oberflächengitter auch ein Dichtegitter entwickelt. Durch die Trennung beider Streuanteile ließ sich die Dynamik der Strukturentstehungen bestimmen. Des weiteren konnte erstmals mit Hilfe der Photoelektronenspektroskopie die molekulare Orientierung an der Oberfläche eines Oberflächengitters nachgewiesen werden. Die Bewegungsursache kann auf einen Impulsübertrag während der Isomerisierung zurückgeführt werden, während die Bewegungsrichtung durch den elektrischen Feldvektor festgelegt wird. Die Theorie der Gitterentstehung konnte verbessert werden. / Solid azobenzene containing polymers show a macroscopic material transport under illumination with blue light. A writing setup was constructed at a synchrotron beamline in order to investigate the dynamics of the grating formation. With this setup it was possible to record the grating velocity for the first time simultaneously with x-ray and laser light scattering. <br /> A very good consistency could be achieved between the experiments and a suitable accomodation of the x-ray scattering theory. The theory reveals, that a density grating develops simultaneously with a surface grating. By separation of both parts the dynamics was determined for the density and the surface grating.<br /> The molecular ordering was determined at the surface with photoelectron spectroscopy. A momentum transfer could be identified as the source of the movement while the electric field defines the direction of the movement. The theory of the grating formation was improved.

Im Bereich der medizinischen Diagnostik spielen DNA-Chips eine immer wichtigere Rolle. Dabei werden Glas- oder Silikon-Oberflächen mit Tausenden von einzelsträngigen DNA-Fragmenten, sog. Sonden, bestückt, die mit den passenden DNA-Fragmenten in der zugefügten Patientenprobe verschmelzen. Die Auswertung solcher Messungen liefert die Diagnose für Krankheiten wie z.B. Krebs, Alzheimer oder für den Nachweis pathogener Erreger. Durch fortschreitende Miniaturisierung dieser Meßsysteme können bis zu 40.000 Genfragmente des Menschen in einer einzigen Messung analysiert werden. Neben den DNA-Fragmenten können Bio-Chips auch für andere biologische Komponenten wie Antikörper und Proteine eingesetzt werden, wobei bei letzteren neben der Bindung auch die Aktivität ein wichtiger Diagnoseparamter ist. <br /> <br /> Am Fraunhofer-Institut für medizinische Technik und am Lehrstuhl für Analytische Biochemie der Universität Potsdam wurden im Rahmen einer Doktorarbeit Methoden entwickelt, die es ermöglichen auf nukleinsäuremodifizierten Sensoroberflächen die Aktivität von Proteinen zu messen. Es wurden Nukleinsäuren auf Oberflächen optischer Sensoren verankert. Diese fungierten als Rezeptor für die Proteine sowie auch als Substrat für Restriktionsenzyme, die Nukleinsäuren schneiden und Polymerasen, die Nukleinsäuren synthetisieren und verlängern können.<br /> <br /> Seine Anwendung fand diese Messmethode in der Messung der Aktivität des Proteins Telomerase, das in 90% aller Tumore erhöhte Aktivität gegenüber gesunden Zellen aufweist. Die Vorteile dieses neuen Assays gegenüber älteren Methoden liegt im Verzicht auf radioaktiv-markierten Komponenten und einer deutlich verkürzten Analysezeit. Die Arbeit schliesst mit einem funktionsfähigen Nachweis der Telomeraseaktivität im Zellextrakt von gesunden und kranken Zellen. Der direkte Einfluß von Hemmstoffen auf die Aktivität konnte sichtbar gemacht werden, und steht daher bei der Entwicklung neuer Tumor-Diagnostika und Therapeutika zur Verfügung. / In the field of medical diagnostic the importance of DNA chips is growing continuously. On silica surfaces hundreds of single stranded DNA fragments are immobilised which finally detect the complementary sequences in samples of patients by hybridisation. These methods enable the detection of serious diseases as cancer, Alzheimer's disease or infection by pathogens. Biomolecules as nucleic acids, antibodies and proteins can be used as receptors on the solid surfaces whereas in case of proteins not only the binding but also the activity are of high interest for medical diagnosis.<br /> <br /> In this thesis a biosensoric approach has been developed to determine the activity of nucleic-acid modifying enzymes. Optical sensors as, e.g., the grating coupler, were used to monitor the association and dissociation of unlabeled compounds on the sensor surface in real time, by virtue of evanescent-field. Furthermore sensors based on total internal reflection fluorescence measured the activity of restriction enzymes and polymerases. The general approach included the immobilisation of oligonucleotides which acted as the receptor for the enzymes as well as the substrate for the enzymatic reaction. Enzymes as EcoRI and Klenow were used to establish a model system to measure the activity of DNA-modifying enzymes on optical surfaces. As most nucleic acid detection systems use amplification steps such as polymerase chain reaction (PCR) to increase the amount of the probe the new optical systems facilitated the analysis of the enzymatic activity by measuring the DNA-synthesis or restriction directly.<br /> <br /> These systems were finally used to detect the activity of the telomerase, an enzymatic marker for the cancerous development of cells. In 90% of cancer cells the activity of telomerase is higher than in normal cells. Additionally the increase of the telomerase activity in cells induced by carcinogenic substances was detected. Furthermore no purification steps of the samples were required as all measurements were performed with crude cell extract.<br /> <br /> Also the effect of inhibitors of the telomerase could be shown in real time measurements underlining the potential of this assay for further developments of new cancer therapeutics.

Fibre Bragg gratings (FBGs) are key components for a vast number of applications in optical communication systems, microwave photonics systems, and optical sensors, etc. The main topic of this thesis is fibre Bragg grating fabrication and applications in direct microwave optical filtering, high speed switching and switchable dual-wavelength fibre lasers. First, a brief overview is given about the photosensitivity in optical fibre, basic FBG fabrication techniques, the popular coupled-mode theory for describing fundamental characteristics of FBGs and the Transfer Matrix method for the numerical simulations of complex-structured FBGs. An advanced FBG fabrication system based on the technique of multiple printing in fibre (with a continuous-wave source) has been used to write complex FBGs incorporating phase shifts, apodization and chirp.      A single double-peaked superimposed grating working in reflection can be employed as a direct optical filter for millimetre-wave signals. Bit error rate measurements confirmed that the filter exhibited nearly on-off behaviour in the passband with a 3-dB bandwidth of 2 GHz for a central frequency of 20 GHz, as expected from the optical spectrum reflection. The presented technique can be used in radio-over-fibre systems or simultaneous up-conversion of ultra-wide band signals and filtering. This thesis focused mostly on the research of two 4-cm long Hamming-apodized gratings written in side-hole fibres with internal electrodes. The temperature dependence measurements showed that the birefringence of the component increased with the temperature. Dynamic measurement has shown nanosecond full off-on and on-off switching. During the electrical pulse action, the grating wavelength was blue-shifted for the x-polarization and red-shifted for the y-polarization due to the mechanical stress. Both peaks subsequently experienced a red-shift due to the relaxation of mechanical stress and the increasing core temperature transferred from the metal in many microseconds. All the wavelength shifts of the two polarizations depend quadratically on the electrical pulse voltage and linearly on the pulse duration. Numerical simulations gave accurate description of the experimental results and were useful to understand the physics behind the birefringence switching. Finally, two switchable dual-wavelength erbium-doped fibre lasers based on FBG feedback were proposed. In one method, an overlapping cavity for the two lasing wavelengths and hybrid gain medium in the fibre laser were introduced. Dual-wavelength switching was achieved by controlling the Raman pump power. The other method employed an injection technique and the dual-wavelength switching was controlled by the power of the injection laser. The switching time was measured to be ~50 ms. Detailed characteristics of the dual-wavelength switching in the two fibre lasers were experimentally studied and corresponding principles were physically explained. / QC 20100922

The focus of this work involves optical and RF (radio frequency) applications of novel microactuation and self-assembly techniques in MEMS (Microelectromechanical systems). The scaling of physical forces into the micro domain is favorably used to design several types of actuators that can provide large forces and large static displacements at low operation voltages. A self-assembly method based on thermally induced localized plastic deformation of microstructures has been developed to obtain truly three-dimensional structures from a planar fabrication process. RF applications include variable discrete components such as capacitors and inductors as well as tunable coupling circuits. Optical applications include scanning micromirrors with large scan angles (>90 degrees), low operation voltages (<10 Volts), and multiple degrees of freedom. One and two-dimensional periodic structures with variable periods and orientations (with respect to an incident wave) are investigated as well, and analyzed using optical phased array concepts. Throughout the research, permanent tuning via plastic deformation and power-off latching techniques are used in order to demonstrate that the optical and RF devices can exhibit zero quiescent power consumption once their geometry is set.

The scalability and cost of microelectromechanical systems (MEMS) optical switches are now the important factors driving the development of MEMS optical switches technology. The employment of MEMS in the design and fabrication of optical switches through the use of micromachining fabricated micromirrors expands the capability and integrity of optical backbone networks. The focus of this dissertation is on the design, fabrication, and implementation of a new type of MEMS optical switch that combines the advantages of both 2-D and 3-D MEMS switch architectures. This research presents a new digital MEMS switch architecture for 1×N and N×N optical switches. The architecture is based on a new microassembled smart 3-D rotating inclined micromirror (3DRIM). The 3DRIM is the key device in the new switch architectures. The 3DRIM was constructed through a microassembly process using a passive microgripper, key, and inter-lock (PMKIL) assembly system. An electrostatic micromotor was chosen as the actuator for the 3DRIM since it offers continuous rotation as well as small, precise step motions with excellent repeatability that can achieve repeatable alignment with minimum optical insertion loss between the input and output ports of the switch. In the first 3DRIM prototype, a 200×280 microns micromirror was assembled on the top of the electrostatic micromotor and was supported through two vertical support posts. The assembly technique was then modified so that the second prototype can support micromirrors with dimensions up to 400×400 microns. Both prototypes of the 3DRIM are rigid and stable during operation. Also, rotor pole shaping (RPS) design technique was introduced to optimally reshape the physical dimensions of the rotor pole in order to maximize the generated motive torque of the micromotor and minimize the required driving voltage signal. The targeted performance of the 3DRIM was achieved after several PolyMUMPs fabrication runs. The new switch architecture is neither 2-D nor 3-D. Since it is composed of two layers, it can be considered 2.5-D. The new switch overcomes many of the limitations of current traditional 2-D MEMS switches, such as limited scalability and large variations in the insertion loss across output ports. The 1×N MEMS switch fabric has the advantage of being digitally operated. It uses only one 3DRIM to switch the light signal from the input port to any output port. The symmetry employed in the switch design gives it the ability to incorporate a large number of output ports with uniform insertion losses over all output channels, which is not possible with any available 2-D or 3-D MEMS switch architectures. The second switch that employs the 3DRIM is an N×N optical cross-connect (OXC) switch. The design of an N×N OXC uses only 2N of the 3DRIM, which is significantly smaller than the N×N switching micromirrors used in 2-D MEMS architecture. The new N×N architecture is useful for a medium-sized OXC and is simpler than 3-D architecture. A natural extension of the 3DRIM will be to extend its application into more complex optical signal processing, i.e., wavelength-selective switch. A grating structures have been selected to explore the selectivity of the switch. For this reason, we proposed that the surface of the micromirror being replaced by a suitable gratings instead of the flat reflective surface. Thus, this research has developed a rigorous formulation of the electromagnetic scattered near-field from a general-shaped finite gratings in a perfect conducting plane. The formulation utilizes a Fourier-transform representation of the scattered field for the rapid convergence in the upper half-space and the staircase approximation to represent the field in the general-shaped groove. This method provides a solution for the scattered near-field from the groove and hence is considered an essential design tool for near-field manipulation in optical devices. Furthermore, it is applicable for multiple grooves with different profiles and different spacings. Each groove can be filled with an arbitrary material and can take any cross-sectional profile, yet the solution is rigorous because of the rigorous formulations of the fields in the upper-half space and the groove reigns. The efficient formulation of the coefficient matrix results in a banded-matrix form for an efficient and time-saving solution.

The focus of this work involves optical and RF (radio frequency) applications of novel microactuation and self-assembly techniques in MEMS (Microelectromechanical systems). The scaling of physical forces into the micro domain is favorably used to design several types of actuators that can provide large forces and large static displacements at low operation voltages. A self-assembly method based on thermally induced localized plastic deformation of microstructures has been developed to obtain truly three-dimensional structures from a planar fabrication process. RF applications include variable discrete components such as capacitors and inductors as well as tunable coupling circuits. Optical applications include scanning micromirrors with large scan angles (>90 degrees), low operation voltages (<10 Volts), and multiple degrees of freedom. One and two-dimensional periodic structures with variable periods and orientations (with respect to an incident wave) are investigated as well, and analyzed using optical phased array concepts. Throughout the research, permanent tuning via plastic deformation and power-off latching techniques are used in order to demonstrate that the optical and RF devices can exhibit zero quiescent power consumption once their geometry is set.

The scalability and cost of microelectromechanical systems (MEMS) optical switches are now the important factors driving the development of MEMS optical switches technology. The employment of MEMS in the design and fabrication of optical switches through the use of micromachining fabricated micromirrors expands the capability and integrity of optical backbone networks. The focus of this dissertation is on the design, fabrication, and implementation of a new type of MEMS optical switch that combines the advantages of both 2-D and 3-D MEMS switch architectures. This research presents a new digital MEMS switch architecture for 1×N and N×N optical switches. The architecture is based on a new microassembled smart 3-D rotating inclined micromirror (3DRIM). The 3DRIM is the key device in the new switch architectures. The 3DRIM was constructed through a microassembly process using a passive microgripper, key, and inter-lock (PMKIL) assembly system. An electrostatic micromotor was chosen as the actuator for the 3DRIM since it offers continuous rotation as well as small, precise step motions with excellent repeatability that can achieve repeatable alignment with minimum optical insertion loss between the input and output ports of the switch. In the first 3DRIM prototype, a 200×280 microns micromirror was assembled on the top of the electrostatic micromotor and was supported through two vertical support posts. The assembly technique was then modified so that the second prototype can support micromirrors with dimensions up to 400×400 microns. Both prototypes of the 3DRIM are rigid and stable during operation. Also, rotor pole shaping (RPS) design technique was introduced to optimally reshape the physical dimensions of the rotor pole in order to maximize the generated motive torque of the micromotor and minimize the required driving voltage signal. The targeted performance of the 3DRIM was achieved after several PolyMUMPs fabrication runs. The new switch architecture is neither 2-D nor 3-D. Since it is composed of two layers, it can be considered 2.5-D. The new switch overcomes many of the limitations of current traditional 2-D MEMS switches, such as limited scalability and large variations in the insertion loss across output ports. The 1×N MEMS switch fabric has the advantage of being digitally operated. It uses only one 3DRIM to switch the light signal from the input port to any output port. The symmetry employed in the switch design gives it the ability to incorporate a large number of output ports with uniform insertion losses over all output channels, which is not possible with any available 2-D or 3-D MEMS switch architectures. The second switch that employs the 3DRIM is an N×N optical cross-connect (OXC) switch. The design of an N×N OXC uses only 2N of the 3DRIM, which is significantly smaller than the N×N switching micromirrors used in 2-D MEMS architecture. The new N×N architecture is useful for a medium-sized OXC and is simpler than 3-D architecture. A natural extension of the 3DRIM will be to extend its application into more complex optical signal processing, i.e., wavelength-selective switch. A grating structures have been selected to explore the selectivity of the switch. For this reason, we proposed that the surface of the micromirror being replaced by a suitable gratings instead of the flat reflective surface. Thus, this research has developed a rigorous formulation of the electromagnetic scattered near-field from a general-shaped finite gratings in a perfect conducting plane. The formulation utilizes a Fourier-transform representation of the scattered field for the rapid convergence in the upper half-space and the staircase approximation to represent the field in the general-shaped groove. This method provides a solution for the scattered near-field from the groove and hence is considered an essential design tool for near-field manipulation in optical devices. Furthermore, it is applicable for multiple grooves with different profiles and different spacings. Each groove can be filled with an arbitrary material and can take any cross-sectional profile, yet the solution is rigorous because of the rigorous formulations of the fields in the upper-half space and the groove reigns. The efficient formulation of the coefficient matrix results in a banded-matrix form for an efficient and time-saving solution.

Besides attenuation, dispersion is the major limiting factor in high data rate fiber optical transmission systems. Dispersion compensation techniques have to be deployed in order to increase the data bandwidth or the reach of fiber optical links. Typically fixed value dispersion compensators are used. However at channel bit rates of 40 GBit/s and beyond adjustable residual dispersion compensator modules (DCM) are needed to guarantee an error free transmission under changing environmental conditions. In this thesis dispersion techniques were investigated which exploit the special propagation properties of higher order modes in custom-designed optical fibers. After a short introduction of state-of-the-art dispersion techniques and their parameters (chapter 2) the modeling and calculation of propagation properties of a particular mode in an optical fiber with an arbitrary, rotation-symmetric refractive index profile is shown (chapter 3). A converter from the fundamental mode and back is needed in order to exploit the propagation properties of a higher order mode (HOM). In this work long-period gratings (LPG) were considered as mode converters (chapter 4) as they can excite selective and nearly lossless a higher order mode. The modeling und calculation of these gratings, based on the fiber calculation of chapter 3, is presented in the first part of chapter 4. Afterwards the manufacturing methods developed during this work are introduced. The spectral properties of realized long-period gratings are discussed and the influence of such factors as strain and temperature on tuning the mode conversion is shown. A dispersion compensator type utilizing only the waveguide dispersion of a certain mode in a custom few mode fiber (FMF) is the subject of chapter 5. The working principle, the fiber design process and first measurements of a realized HOM-DCM with almost completely coupling FMF-LPG are presented. Subsequently the principle of a novel dispersion compensator with an arbitrary dispersion function for a higher or the fundamental mode is explained. In chapter 6 another type of dispersion compensator is investigated consisting of equally distributed long-period gratings along an optical fiber. The fiber pieces between the gratings create a certain time delay between the fundamental mode and the considered higher order mode. It is shown in simulations and in an experiment, that by tuning the mode conversion of each grating and the optical phase relation between the two signal paths in each fiber piece this finite impulse filter structure is so adjusted to function as a tunable residual dispersion compensator.

High efficiency photovoltaic solar cells are expected to continue to be important for a variety of terrestrial and space power applications. Solar cells made of optically thick materials often cannot meet the cost, efficiency, or physical requirements for specialized applications and, increasingly, for traditional applications. This dissertation investigates improving the performance of single-junction GaAs solar cells by incorporating InGaAs/GaAs quantum wells and quantum dots to increase their spectral response bandwidth, and by incorporating structures that confine light in the devices to improve their absorption of it. InGaAs/GaAs quantum dots-in-wells extend the response of GaAs homojunction devices to wavelengths >1200 nm. Nanoparticles that are randomly deposited on the top of optically thick devices scatter light into waveguide modes of the device structures, increasing their absorption of electromagnetic energy and improving their short-circuit current by up to 16%. Multiply periodic diffractive structures have been optimized using rigorous software algorithms and fabricated on the back sides of thin film quantum dot-in-well solar cells, improving their spectral response at wavelengths 850 nm to 1200 nm, where only the quantum dot-in-well structures absorb light, by factors of up to 10. The improvement results from coupling of diffracted light to waveguide modes of the thin film device structure, and from Fabry-Perot interference effects. Simulations of absorption in these device structures corroborate the measured results and indicate that quantum well solar cells of ~2 µm in thickness, and which are equipped with optimized backside gratings, can achieve 1 Sun Airmass 0 short-circuit current densities of up to ~5 mA/cm2 (15%) greater than GaAs homojunction devices, and of up to >2 mA/cm2 (7%) greater than quantum well devices, with planar back reflectors. A combination of Fabry-Perot interference and diffraction into waveguide modes of the thin devices is shown to dominate the simulated device response spectra. Simulations also demonstrate the importance of low-loss metals for realizing optimal light trapping structures. Such device geometries are promising for reducing the cost of high efficiency solar cells that may be suitable for a variety of traditional and emerging applications. / text

Current technology used to diagnose hearing disorders is limited. This is mostly due to the fact that the auditory structures are very small and not easily accessible with existing imaging technologies. The objective of this dissertation was to investigate the potential of high-frequency ultrasound as a tool for exploring the anatomy of the auditory system. Three studies were conducted in order to demonstrate the feasibility of high-frequency ultrasound as a diagnostic technology for hearing disorders. In the first study, an in-house developed 50 MHz annular array-based ultrasound system was used to provide ex-vivo images of auditory structures in cadaveric temporal bones. It was shown that the spatial resolution was sufficient to visualize a high level of detail of the ossicular bones of the middle ear as well as intra-cochlear structures of the inner ear. In the second study, a 50 MHz 1.26? pitch phased array ultrasound transducer was designed for imaging intra-cochlear structures through the round window membrane. As this element pitch results in large grating lobe artifacts, novel transmit beamforming techniques were developed to suppress grating lobes resulting from this large-pitch array. Theoretical techniques using the impulse-response simulation method and experimental verification using high-frequency linear array ultrasound system (Vevo 2100, VisualSonics, Canada) showed that these techniques were able to suppress grating lobe levels up to 40 dB. In the third study, a needle mounted 45 MHz single-element ultrasound probe was fabricated in order to measure the vibrations of intra-cochlear structures on human cadavers. Basilar membrane velocimetry measurements were successfully performed using pulsed-wave Doppler ultrasound in the frequency range between 100 Hz-2 KHz. The measured velocity of the basilar membrane and the round window membrane showed that the middle ear resonance frequency near 1 KHz was present over multiple temporal bones. This is the first work that has explored the human auditory system with high resolution ultrasonic visualization and Doppler velocimetry.