Magister Philosophiae - MPhil / Agriculture and livestock production were the mainstay of the economies of many sub-Saharan African countries, including of Cameroon, in the 1970s. Things began to change with the discovery of petroleum products and natural minerals, and the push to industrialise. This led to a shift from agricultural production to other more 'beneficial' sectors. In the 1990s there was an 'imposed' liberalisation of the agricultural sector. This liberalisation was marked by a disengagement of most governments in developing countries from assisting agriculture. In Cameroon, disengagement was achieved by the promulgation of law No. 92/006 of 14th August 1992 and its decree of application No. 92/455/PM of 23rd November. This law encouraged the creation of common initiative groups which could independently pool their resources to increase agricultural production. Although there has been a shift to non-agricultural sectors in many sub- Saharan countries, on the whole, however, many rural areas in these nations have remained essentially agro-pastoral. Unfortunately some rural areas, like Small Babanki in Cameroon, whose livelihoods are land-based are faced with soil erosion, population pressure and farmer/grazer conflicts which undermine the little economic gains made in these places. Rural-dwellers have resorted to several innovations to circumvent these constraints to agricultural production. Objectives of the research This research focused on an indigenous agricultural innovation called Cameroon. The innovation involves a partnership between farmers and grazers whereby farmers build paddocks around their farms, and cattle herders drive their herds into farms where they deposit their manure. The idea of the practice is that it enriches the soil of the farms, provides the herds access to good fodder, and reduces conflicts between crop farmers and grazers. The objective of the study was to investigate the role this agricultural innovation is playing in reducing poverty and farmer/grazer conflicts. The research focused on a community called Small Babanki located in the North-West Region of Cameroon. Research questions To gain accurate insight into the role played by NPM, answers were sought to the following questions: (i) What are the causes of farmer/grazer conflicts in Small Babanki and how are these conflicts manifested? (ii) What efforts have been made hitherto by stakeholders to resolve farmer/grazer conflicts and what were the outcomes of such interventions? (iii) What motivated the development of the NPM farming system and how does the system function? (iv) How has the introduction of NPM affected the occurrence of conflicts and the resolution of conflicts when they occur? (v) Has NPM contributed to increasing the output, income and the market value of the products of farmers and grazers? (vi) How have the asset bases of grazers and farmers changed and what additional livelihood options are available to them as a result of adopting NPM? (vii) What are the major constraints that hinder practitioners of the innovation from getting maximum returns from it? Data collection method The data collection method for this research involved a review of reports written on the innovation by NGOs promoting it. Within Small Babanki, a focus group discussion was held with farmers and an elaborate questionnaire was administered amongst 10 randomly selected farmer households and 10 randomly selected grazer households. Structured interviews were also held with key informants, such as local officials and traditional and religious leaders. Findings and conclusions The research supports the perception that, in Small Babakni, NPM benefits both crop farmer and grazer households by means of improving productivity, reducing poverty, and reducing conflict. Respondents indicated that generally speaking the asset base and livelihood options of practitioners of NPM are constantly improving. They also stressed that improvements in education, health, nutrition, land tenure and safety of shelter are indicative of the amelioration of their state of wellbeing, and this in large measure can be attributed to the adoption of NPM Measurement of poverty using the both the US$I/day and the national poverty line of 503.19 Cf'Azday revealed that cropping households are living on the fringes of poverty while grazing households tend to live just above the poverty line. However, going by information provided by respondents on their outputs, the proportion of what was consumed by the household, the proportion of what was sold and the proportion of what was retained enabled us to make an estimation of the cash and noncash incomes of both grazing and cropping households. It was discovered that at any point they seemed to have a significant noncash wealth reserve which could easily be converted into cash in order to meet daily expenses. Both the declarations of respondents and some official documentation suggest that NPM was contributing positively towards a reduction of conflicts between farmers and grazers in Small Babanki.

Dans les zones euphotiques des écosystèmes côtiers, les producteurs primaires se développent à la fois dans l’espace pélagique (colonne d’eau) et benthique (sur ou près du fond). Dans ces écosystèmes, le couplage bentho-pélagique prend une place non négligeable qu’il convient d’étudier. Les consommateurs primaires benthiques en sont des acteurs à travers leur activité alimentaire. La relation trophique entre les différents producteurs primaires et les consommateurs primaires benthiques reste toutefois mal élucidée. Pour apporter des éléments d’informations, cette thèse s’est focalisée dans un premier temps sur la dynamique spatio-temporelle des producteurs primaires au sein de la matière organique particulaire en suspension (MOPS), notamment au niveau de l’interface eau-sédiment. Cette étude a permis de montrer une forte variation saisonnière de ce peuplement algal à toutes les profondeurs, mais également une importante contribution relative des diatomées benthiques dans la MOPS près du fond en hiver. Le second axe de recherche s’est concentré sur la relation trophique entre des consommateurs primaires benthiques (coquille Saint-Jacques, pétoncle noir, moule et ormeau) et les producteurs primaires présents dans la MOPS. L’analyse des isotopes stables dans les tissus mous des pétoncles noirs et des moules a indiqué une prédominance du phytoplancton assimilé et une potentielle contribution du microphytobenthos dans leur régime alimentaire (plus importante pour la moule), notamment en période hivernale et près du fond. Cependant, ces études ont mis en lumière l’effet du métabolisme sur les valeurs isotopiques dans les tissus mous. Un aspect novateur de cette thèse a été d’utiliser les exosquelettes des coquilles Saint-Jacques et des ormeaux pour caractériser leur régime alimentaire dans le milieu naturel, afin de s’affranchir des problèmes liés à l’utilisation des isotopes stables dans les tissus mous. Des études en conditions contrôlées ont validé l’intégration des sources d’alimentation à travers le carbone métabolique dans les carbonates de la coquille Saint-Jacques et la couleur de la coquille pour l’ormeau. Des études complémentaires doivent toutefois être menées. Enfin, une dernière étude s’est focalisée sur la capacité de la coquille Saint-Jacques à mettre en suspension les particules benthiques par leurs mouvements de valves. / In coastal euphotic areas, primary producers grow both in the pelagic (water column) and in the benthic zone (in or near the sediment). In these ecosystems, benthic-pelagic coupling is an important process which must be well described. Benthic primary consumers modify this coupling through their diet. Trophic relationships between primary producers and benthic primary consumers are not thoroughly understood. To gain additional information, this thesis was focused on the spatial and temporal dynamic of primary producers in suspended particulate organic matter (SPOM), especially at water-sediment interface. This study highlighted a seasonal variation of microalgal populations at all depths, and a relative dominance of benthic diatoms in SPOM near sediment and in winter. The second objective of the thesis was focused on the trophic relationships between benthic primary consumers (great scallop, black scallop, mussel and ormer) and primary producers in SPOM. Stable isotope analysis in soft tissues of black scallops and mussels showed a predominance of assimilated phytoplankton and the potential contribution of microphytobenthos in their diet (more important for mussel), especially in winter and near the sediment. However, this study highlighted the impact of metabolism on stable isotopic values of soft tissues. An innovative aspect of this thesis was the use of exoskeleton of great scallops and ormers to characterize their diet in natural environment and thus overcome problems related to the utilization of stable isotopes in soft tissues. Experiments in controlled conditions validated food sources integration through metabolic carbon in carbonates of great scallops and shell color for ormer but complementary studies are required on mollusk shell utilization. The last part of this thesis was focused on the ability of great scallops to achieve resuspension of benthic particles by valve movements.

The factors that determine the efficiency of energy transfer in aquatic food webs have been investigated for many decades. The plant-animal interface is the most variable and least predictable of all levels in the food web. In order to study determinants of food quality in a large lake and to test the recently proposed central importance of the long-chained eicosapentaenoic acid (EPA) at the pelagic producer-grazer interface, we tested the importance of polyunsaturated fatty acids (PUFAs) at the pelagic producerconsumer interface by correlating sestonic food parameters with somatic growth rates of a clone of Daphnia galeata. Daphnia growth rates were obtained from standardized laboratory experiments spanning one season with Daphnia feeding on natural seston from Lake Constance, a large pre-alpine lake. Somatic growth rates were fitted to sestonic parameters by using a saturation function. A moderate amount of variation was explained when the model included the elemental parameters carbon (r2 = 0.6) and nitrogen (r2 = 0.71). A tighter fit was obtained when sestonic phosphorus was incorporated (r2 = 0.86). The nonlinear regression with EPA was relatively weak (r2 = 0.77), whereas the highest degree of variance was explained by three C18-PUFAs. The best (r2 = 0.95), and only significant, correlation of Daphnia's growth was found with the C18-PUFA α-linolenic acid (α-LA; C18:3n-3). This correlation was weakest in late August when C:P values increased to 300, suggesting that mineral and PUFA-limitation of Daphnia's growth changed seasonally. Sestonic phosphorus and some PUFAs showed not only tight correlations with growth, but also with sestonic α-LA content. We computed Monte Carlo simulations to test whether the observed effects of α-LA on growth could be accounted for by EPA, phosphorus, or one of the two C18-PUFAs, stearidonic acid (C18:4n-3) and linoleic acid (C18:2n-6). With >99 % probability, the correlation of growth with α-LA could not be explained by any of these parameters. In order to test for EPA limitation of Daphnia's growth, in parallel with experiments on pure seston, growth was determined on seston supplemented with chemostat-grown, P-limited Stephanodiscus hantzschii, which is rich in EPA. Although supplementation increased the EPA content 80-800x, no significant changes in the nonlinear regression of the growth rates with α-LA were found, indicating that growth of Daphnia on pure seston was not EPA limited. This indicates that the two fatty acids, EPA and α-LA, were not mutually substitutable biochemical resources and points to different physiological functions of these two PUFAs. These results support the PUFA-limitation hypothesis for sestonic C:P < 300 but are contrary to the hypothesis of a general importance of EPA, since no evidence for EPA limitation was found. It is suggested that the resource ratios of EPA and α-LA rather than the absolute concentrations determine which of the two resources is limiting growth.

Benthic filter feeders (BFF) can reduce phytoplankton concentration (abundance) thereby controlling eutrophication in several ecosystems, including rivers. However, experiments suggest warming can alter the relationship between BFF grazing rate and the growth rate of (heterotrophic) planktonic prey. To investigate how eutrophication control by grazers is altered with temperature under the influence of other important abiotic (water depth, and speed, light, and turbidity) and biotic factors (initial phytoplankton concentration [hereafter: Pin value], BFF density and spatial BFF distribution), we developed a spatially-explicit computer simulation model. This model simulates the dynamics of a phytoplankton population traveling through a simplified river channel while being grazed by BFF. Our model includes the thermal responses of BFF grazing and phytoplankton growth. The results show that BFF grazing can qualitatively alter and, in some circumstances, even reverse the response of phytoplankton to warming. Moreover, the response of grazer-controlled phytoplankton to warming, water depth and Pin value is non-linear and phytoplankton can increase steeply with slight changes within some ranges of these variables. In addition, these variables can interact causing their combined effects on eutrophication to differ from what is expected considering their isolated effects. Generally, the effect of most variables, including temperature, Pin value and BFF density and spatial distribution, is larger at shallow waters. Moreover, our study shows that phytoplankton control can be substantially improved by heterogeneous BFF distributions where the BFF are located at the extremes of the river either upstream or downstream instead of homogenously distributed along the whole river. However, warming can cause a switch between these two optimal distributions or even can cause differences among the spatial distributions to disappear. In general, the homogeneous BFF distribution can be used as conservative estimate of eutrophication control. In conclusion, this work shows that trophic control can qualitatively alter the response of eutrophication to warming, supporting previous studies suggesting that the prediction of global warming effects requires considering not only the thermal responses of organisms but also their trophic interactions. In addition to these biotic variables, this thesis reveals that considering the interactions between abiotic and biotic variables and including their spatial distribution are important for eutrophication control. Especially, the detection of thresholds in the response of grazer-controlled phytoplankton to temperature, water depth, Pin value, and spatial BFF distribution indicates that one should be careful with predictions because of potential abrupt changes. Although further studies are needed to make specific recommendations for water quality management, our work provides preliminary suggestions on the conditions where grazers or Pin reductions can be more efficient to control eutrophication.

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En raison d’une utilisation croissante et massive, les nanoparticules manufacturées apparaissentcomme de potentiels contaminants émergents pour l’environnement, incluant notammentles écosystèmes aquatiques. Alors que le transfert trophique semble constituer unevoie d’exposition majeure pour les organismes, une connaissance lacunaire dans la littératurescientifique persiste, résultant pour partie des difficultés expérimentales inhérentes àce type d’exposition. Pour ce travail en conditions contrôlées de laboratoire, les nanoparticulesd’or (sphériques, 10 nm, fonctionnalisées aux PEG-amines), stables en solution, ontété choisies pour l’étude du transfert trophique et des impacts toxiques sur des organismesaquatiques. Ce continuum trophique considère la base des réseaux trophiques (biofilms naturels,algues), des niveaux intermédiaires (poissons brouteurs, bivalves suspensivores), jusqu’auxorganismes de haut de chaînes trophiques, avec l’anguille européenne. Avec des expositionsréalisées à de relatives faibles doses, ce travail tend à la représentativité environnementale.Des approches méthodologiques intégratives des niveaux subcellulaire à tissulaire(RT-qPCR, séquençage haut-débit, histologie) ont permis d’évaluer les impacts toxiques.Les résultats indiquent une importante capacité de rétention des nanoparticules par les biofilmsnaturels. À la suite d’une exposition de 21 jours, les dosages d’or révèlent un transfertdes biofilms aux poissons brouteurs, avec une distribution de l’or dans tous les organes. Deplus, ce transfert est associé à une réponse inflammatoire au regard des lésions histologiquesobservés dans les foies, rates et muscles des poissons exposés. Une chaîne alimentaire « naturelle» à trois maillons trophiques, impliquant algues - bivalves - anguilles européennes,atteste d’un transfert significatif jusqu’au poisson prédateur. Enfin, l’analyse du transcriptome,par une approche de séquençage haut-débit, des foies et cerveaux d’anguilles exposéesaux nanoparticules par nourriture enrichie, a permis de mettre en évidence une réponseconjointe à ces deux organes dans des processus biologiques associés au système immunitaireet sa régulation, dont des récepteurs NOD-like impliqués dans l’inflammasome.L’ensemble des résultats expérimentaux interpellent quant aux effets délétères à long-termequ’engendreraient les nanoparticules sur les écosystèmes aquatiques, illustrant par ailleursla propension de ces contaminants à être transférés dans les chaînes trophiques. / Due to an increasing and massive use, engineered nanoparticles are raising as potentialemerging contaminants in the environment, including aquatic ecosystems. While trophictransfer appears to constitute a major exposure route for organisms, scientific literature hasdifficulties to respond to the questions raised to explore the range of the interactions existingbetween nanoparticles and living organisms at different scales from the trophic interactionsto the cellular impacts. This problem is partly due to experimental difficulties inherent tothis exposure type. For this work performed in controlled laboratory conditions, sphericalgold nanoparticles (10 nm, coated with PEG-amines, positively charged) were chosen tostudy the trophic transfer and toxic effects on aquatic organisms. Trophic chains concernedseveral trophic levels (up to three) with a variety of species considered : the basis of thetrophic web with natural biofilms or microalgae, intermediate levels with grazing fish orsuspensivorous bivalves, and up to top food chain organisms, with the European eel, a carnivorousfish.With relatively low doses for exposures, this work tends to represent environmentalconditions. Integrative methodological approaches from subcellular to tissue levels(RT-qPCR, RNA-sequencing, histology) were performed in order to assess toxic impacts.The results indicate a high retention capacity of nanoparticles by natural biofilms. Followinga 21-day exposure, gold quantifications reveal a transfer from biofilms to grazing fish, witha gold distribution in all organs. Moreover, this transfer is associated with an inflammatoryresponse according to the histological lesions observed in the liver, spleen and muscle ofexposed fish. A longer food chain, with three trophic levels involving microalgae - bivalves- European eels, is set up to give a better representation of the complexity of trophic interactionsin the aquatic environment. It shows a significant transfer to the predatory fish.Transcriptomic analyses, using the RNA-sequencing approach, for the liver and the brain ofexposed eels by nanoparticles’ enriched food, highlight a joint response for these two organsin the biological processes associated with the immune system and its regulation, includingNOD-like receptors involved in inflammasome.All the experimental results suggest long-term harmful effects that nanoparticles would generatein aquatic ecosystems, emphasizing the ability of these contaminants to be transferredthroughout trophic chains.