• Refine Query
  • Source
  • Publication year
  • to
  • Language
  • 58
  • 18
  • 17
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • Tagged with
  • 117
  • 41
  • 29
  • 23
  • 17
  • 16
  • 15
  • 15
  • 15
  • 11
  • 10
  • 9
  • 8
  • 8
  • 7
  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
71

Synthesis and processing of nitrogen-doped carbon nanotubes as metal-free catalyst for H2S selective oxidation process / Synthèse et mise en forme des catalyseurs «sans métaux» à base de nanotubes de carbone dopés à l’azote pour les procédés d’oxydation sélective de l’H2S

Duong-Viet, Cuong 23 July 2015 (has links)
Les nanotubes de carbone (NTCs) dopés avec différents hétéroéléments tels que l'oxygène et l'azote connaissent un intérêt croissant dans le domaine de la catalyse hétérogène comme leur utilisation en tant que catalyseur sans métaux. L'objectif de cette thèse consiste à la synthèse et la caractérisation de matériaux catalytiques à base de NTC dopés à l'azote supportés sur du carbure de silicium SiC par voie in situ (CVD) et ex-situ méthodes. Une autre approche a été utilisée pour la fonctionnalisation de la surface des nanotubes de carbone et basées sur l'utilisation d'un agent oxydant (HNO3) en phase gazeuse. Ce procédé d'oxydation crée non seulement des défauts sur la surface des nanotubes de carbone mais également décore leur surface avec des groupes fonctionnels oxygénés. Les NTCs dopés à l'oxygène et à l'azote ainsi synthétisés ont été caractérisés par différentes techniques (XPS, MEB, MET, BET, ATG). Ces catalyseurs carbonés présentent des performances remarquables en termes d'activité et de sélectivité en soufre, et une très grande stabilité sous flux en fonction du temps dans la réaction d'oxydation partielle de l'H2S en soufre élémentaire, et ce, même à vitesse spatiale de gaz élevée (WHSV) et faible rapport O2/H2S. L'influence des différentes propriétés physico-chimiques et les défauts présents sur la surface des NTC sur les performances catalytiques ont été étudiée et discutée dans le cadre de ce travail. / Carbon nanotubes (CNTs) containing different doping such as oxygen and nitrogen composites have received more and more scientific attention as metal-free catalyst in the field of heterogeneous catalysis. The aim of this thesis is related to the synthesis and characterization of nitrogen-doped CNTs decorated on SiC support using both in-situ (CVD with NH3) and ex-situ (N@C) methods. Other approach was employed for the surface functionalization of CNTs is based on the use of oxidative agent (HNO3) in gas phase. This oxidation process not only creates defects on the CNTs surface but also decorates their surface with oxygenated functional groups. The as-synthesized oxygen- and nitrogen-doped CNTs are characterized by different techniques (XPS, SEM, TEM, BET, TGA). The carbon based catalysts exhibit outstanding performances in term of activity and sulfur selectivity, and very high stability with time on stream in the partial oxidation of H2S into elemental sulfur even at high gas space velocity (WHSV) and low O2-to-H2S molar ratio. The influence of the physical-chemical properties and defects present on the CNTs surface on the catalytic performance was thoroughly investigated and discussed within the framework of this work.
72

Novel Orally Active Hydrogen Sulfide-Releasing Compound, SG1002, Improves Left Ventricular Function and Survival in a Murine Model of Ischemic Cardiomyopathy

Balan, Bharat 01 January 2017 (has links)
Hydrogen sulfide (H2S) is a gasotransmitter that has shown cardioprotective effects in the setting of myocardial injury such as acute myocardial infarction (MI) and pressure overload-induced heart failure. However, there are shortcomings in precision and control release from the use of traditional formulations of H2S in the form of inorganic salts. In this thesis, we sought to determine if the novel, orally active, slow-releasing H2S-compound SG1002 plays a role in attenuating MI-induced left ventricular (LV) dysfunction and adverse remodeling. We also evaluated the effect of SG1002 on changes in ECG parameters such as QT interval, in addition to 28-day survival post MI. SG1002 protects against ischemic cardiomyopathy in mice by mitigating LV dysfunction as measured by echocardiography and decreasing LV scar size as measured by histopathological methods. The improvement in survival might be due to the reduction in QT interval prolongation hence lessening the likelihood of forming lethal arrhythmias post MI. Western blot analyses of SG1002 treated mice showed restoration of VEGF levels indicating a pivotal role played by pro-angiogenic signaling in the improvement of cardiac function and attenuation of adverse remodeling. We propose that SG1002 can be a promising pharmacotherapeutic means for the treatment of ischemic heart failure.
73

A prototype dynamic model for the co-treatment of a high strength simple-organic industrial effluent and coal-mine drainage

Harding, Theodor 25 January 2021 (has links)
This research study's the use of biological sulfate reduction technologies for the treatment of Sasol Secunda's coal-mine drainage (CMD) using Fischer-Tropsch Reaction Water (FTRW) as a cost-efficient carbon source. The research aims to develop a prototype dynamic model that describes this co-treatment of FTRW and CMD in both a continuously stirred tank reactor (CSTR) biological sulfate reduction (BSR) system and a BSR gas-lift (BSR-GL) integrated system. The BSR-GL system recovers elemental sulfur (S0 ) from the H2S produced and stripped from the BSR unit. Furthermore, this study aims to use the prototype model for a quantitative comparison of the CSTR-BSR and BSR-GL systems. Two bench-scale 5-litre CSTR-BSR and a 20-litre BSR-GL system were operated, under varying feed COD concentrations and hydraulic retention times (HRTs), to generate datasets for use in verification and a rudimentary validation of the prototype model. The BSR-GL integrated system includes 1) a 1-litre H2S gas reactive absorption (ABS) unit utilising an aqueous ferric solution for the recovery of elemental sulfur (S0 ) from sulfide and 2) ferrous biological oxidation reactor to regenerate ferric from the ferrous for re-supply to the ABS unit. The datasets generated in the experimental study allowed for the identification, mathematical modelling and reaction verification of 32 components that interact as reactants and products in 23 reactions observed in the two BSR systems. The prototype model is presented in a mass and charge balanced Gujer matrix that includes, i) 5 SRB mediated processes, ii) 2 liquid-gas mass transfer processes, iii) 3 processes describing the ABS and Fe2+ bio-oxidation units, iv) 4 processes describing sulfide and elemental sulfur oxidation and v) the S0 and poly-sulfide aqueous equilibrium and vi) 9 processes describing death regeneration and BPO hydrolysis. This prototype model was implemented in the DHI WEST® software for initial stage simulation trials. The experimental datasets allowed for the first-stage estimation of the best-fit reaction rate equations and the calibration of the kinetic parameters related to the 23 reactions, using MATLAB® curve fitting toolbox. A pre-processor that describe the pH and equilibrium chemistry of the components of the artificially prepared FTRW+CMD feed mixture batches under varying total concentrations have also been developed in this research. This was done to generated influent file to the DHI WEST® simulations that incorporated the dynamics related to the FTRW+CMD feed mixtures. The sulfate utilisation rate (gSO4 -2 .l-1 .d-1 ) of the GL-BSR and CSTR-BSR systems were compared to determine which system had the best sulfate removal. The results were found to be as follows; a. On comparison it was found that the sulfate substrate utilisation rate for the CSTR_BSR system is 39.28% of that of the BSR-GL_N2 system, where both systems were fed at feed mixture of COD of 2500mgCOD/l, where the COD:SO4 2- was 0.7, b. For the same systems fed a feed mixture of COD at 5000mgCOD/l (COD:SO4 2- = 0.7), the sulfate substrate utilisation rate for the CSTR_BSR system was found to be 17.86% less than that of the BSR_GLN2 system. c. Finally, it was also found that the substrate utilisation rate for the CSTR_BSR system was 30.06% less than that of the BSR_GLN2 system at Se of 4gCOD/l, for both systems fed substrate at 5000mgCOD/l. Thus, it can be concluded that the sulfate substrate utilisation rate for the BSR-GL system is higher than that of the CSTR_BSR system, for systems fed COD feed mixtures at 2.5 or 5gCOD/l where both systems have the same effluent substrate concentrations. However, the difference in the comparative substrate utilisation rate is less at higher feed substrate concentrations. This is the influence of substrate inhibition on the active SRB biomass, which increases with higher effluent substrate concentrations. Finally, this research found that the use of gas-lift reactor technologies is superior to CSTR technologies in the treatment of coal-mine drainage utilising biological sulfate reduction (BSR). The CSTR-BSR system, fed sulfate between 1.6 to 14gSO4 2- /l, produced effluent with high dissolved H2S concentrations, on average 285mgS/l and maximum at >600mgS/l. Releasing this effluent to the environment would be hazardous to aquatic and human health and corrosive to infrastructure. As such, the effluent from the CSTR-BSR system requires further treatment to stabilise the water for any use. The BSR-GL technology allows for the conversion of the H2S produced during BSR reactions to form elemental sulfur, which is a resource recovered from this process, thus complying to the circular economy aim of this study.
74

Electrochemical Behaviors of the Electrodes for Proton Conducting Intermediate Temperature Solid Oxide Fuel Cells (IT-SOFC)

Sun, Shichen 22 October 2018 (has links)
Proton conducting intermediate temperature (600oC-400oC) solid oxide fuel cells (IT-SOFC) have many potential advantages for clean and efficient power generation from readily available hydrocarbon fuels. However, it still has many unsolved problems, especially on the anode where the fuel got oxidized and the cathode where oxygen got reduced. In this study, for the anode, the effects of hydrogen sulfite (H2S) and carbon dioxide (CO2) as fuel contaminants were studied on the nickel (Ni) based cermet anode of proton conducting IT-SOFC using proton conducting electrolyte of BaZr0.1Ce0.7Y0.1Yb0.1O3 (BZCYYb). Both low-ppm level H2S and low-percentage level CO2 caused similar poisoning effects on the anode reaction. The H2S poisoning effect was also found to be much less than on oxide-ion conducting SOFC, which is attributed to the absence of water evolution for the anode reaction in proton conducting SOFC. In addition, the H2S/CO2 poisoning mechanisms were investigated using X-ray diffraction, energy dispersive spectroscopy (EDS), Raman spectroscopy, and secondary ion mass spectroscopy (SIMS). For H2S, other than possible sulfur dissolution into BZCYYb, no bulk reaction was found, suggesting sulfur adsorption contributes to the reduced performance. For CO2, reaction with BZCYYb to form BaCO3 and CeO2 is identified and is believed to be the reason for the sudden worsening in CO2 poisoning as temperature drops below ~550oC. For the cathode, several representative SOFC cathodes including silver (Ag), La0.6Sr0.4Co0.2Fe0.8O3-δ (LSCF), LSCF-BZCYYb composite, and Ba0.5Sr0.5Co0.8Fe0.2O3-δ (BSCF) were evaluated based on BZCYYb electrolyte. LSCF give similar high interfacial resistance as Ag, while LSCF-BZCYYb composite cathode shows lower interfacial resistance, suggesting LSCF behaves like pure electronic conductor cathode in this case. For BSCF, it shows smallest interfacial resistance and the charge transfer process appears to accelerate with the introduction of H2O, while oxygen adsorption/transport seem to slow down due to adsorbed H2O. Furthermore, CO2 was shown to cause poisoning on the BSCF cathode, yet the poisoning was significantly reduced with the co-presence of water. The results suggest that although BSCF seem to display mixed proton-electronic conduction, its strong affinity to H2O may inhibit the oxygen reduction reaction on the cathode and new cathode materials still need to be designed.
75

Nickel exsolution effect on the catalytic behavior of ruddlesden-popper manganites in sofc conditions using colombian natural gas

Vecino Mantilla, Juan Sebastián 02 September 2020 (has links)
[EN] Several major problems have to be solved before Solid Oxide Fuel Cells (SOFC) can operate continuously using hydrocarbon fuels such as natural gas. The risk of low catalytic behavior for fuel reforming, the carbon formation/deposition on the anode material at high operating temperatures and the presence of impurities in the fuel (in particular sulfides) can dramatically reduce the performance and durability of the cells. Taking all this into account, new anode materials with adequate (electro)catalytic properties are required. Recently, manganite compounds with Ruddlesden-Popper (RP) structure have been studied as potential new anode materials in INTERFASE group at Universidad Industrial de Santander (UIS). Their electrochemical performance have been described in previous works with promising results, but a fundamental knowledge was missing concerning the catalytic properties of such materials and the way to improve them by the addition of nickel metallic particles on the electrode surface. The current Ph.D. thesis was focused on the synthesis, characterization and catalytic study for steam reforming in SOFC anode conditions (low steam content) of a new RP manganite (La1.5Sr1.5Mn1.5Ni0.5O7±δ), which, in reducing atmosphere at high operating temperatures promotes via an exsolution mechanism the formation of two phases, i.e. an RP manganite of composition LaSrMnO4±δ decorated with metallic active Ni nanoparticles embedded in the surface; such strategy can be viewed as an original way to improve the (electro)catalytic properties of the anode materials and then a promising option for future SOFC systems operating with Colombian natural gas. The first chapter deals with the synthesis and characterization of the RP n= 2 phase La1.5Sr1.5Mn1.5Ni0.5O7±δ using the Pechini method. In agreement with SOFC operating temperature, Ni exsolution has been studied in diluted H2 at different temperatures (750, 800 and 850 °C) and reduction times. Ni nanoparticles decorating an RP n= 1 manganite is confirmed by XRD, TEM-EDS analysis and the size of the metallic particles on the oxide surface, below 100 nm, is characterized as a function of the exsolution conditions. The second chapter presents the catalytic behavior for the methane steam reforming reaction of the exsolved material applying the Gradual Internal Reforming concept adapted to SOFC operation (i.e. low water content, steam to carbon ratio equal to 0.15) at different reaction temperatures (750, 800 and 850 °C). The catalytic properties of Ni impregnated samples using a similar (La,Sr)2MnO4±δ ceramic support are also presented for comparison. The exsolved material exhibits better performance than the impregnated manganite for the reaction, especially at 850 °C, with higher conversion, conversion rate, and H2 production rate. Concerning the steam reforming of light alkane gas mixtures (CH4-C2H6, and CH4-C3H8), the behavior is affected due to the competition between the molecules and low available metallic active sites, but without affecting the H2 production. In addition, at long reaction times, the activity over the exsolved material is stable even with 100 h of reaction, without formation of carbonaceous species on the Ni particles, as confirmed by TEM and TGA/MS analysis. In the third and last chapter, the possible coke formation and sulfide poisoning are presented. Despite the high and stable catalytic behavior for methane steam reforming reaction with considerable carbon formation resistance, the exsolved material exhibits a high level of sensitivity to H2S poisoning, similar to the case of state-of-the-art Ni/YSZ anodic cermet and or Ni impregnated catalyst, with a drop of the activity to almost zero. Nevertheless, the exceptional overall results obtained for the exsolution-based material are promising for a possible use as SOFC anode operating with sulfur-free Colombian natural gas. / [ES] Muchos son los problemas que deben resolverse antes de que las celdas de combustible de óxido sólido (SOFC por sus siglas en inglés) puedan operar continuamente usando combustibles hidrocarbonados como por ejemplo el gas natural. El riesgo de una baja actividad catalítica para el reformado del combustible, la formación y depósito en el material de ánodo a elevadas temperaturas de operación y la presencia de impurezas en el combustible empleado (en particular de sulfuros) pueden reducir dramáticamente el desempeño y la durabilidad de las celdas. Teniendo todo esto en cuenta, nuevos materiales de ánodo con adecuadas propiedades (electro)catalíticas son necesarios. Recientemente, en el grupo INTERFASE de la Universidad Industrial de Santander (UIS), compuestos de tipo manganita con estructura Ruddlesden-Popper (RP) han sido estudiados como potenciales materiales de ánodo. Su desempeño electrocatalítico ha sido descrito en trabajos previos con promisorios resultados, pero el conocimiento fundamental sobre las propiedades catalíticas de dichos materiales y la forma de mejorarlos mediante la adición de partículas metálicas de níquel en la superficie del electrodo aún faltaba. La presente tesis doctoral se enfocó en la síntesis, caracterización y estudio catalítico en el reformado con vapor en condiciones de ánodo de celdas SOFC (bajo contenido de vapor) de una nueva manganita de tipo RP (La1.5Sr1.5Mn1.5Ni0.5O7±δ), la cual, en atmósfera reductora y a elevadas temperaturas de operación, promueven a través del mecanismo de exsolución la formación de dos fases: una manganita tipo RP de composición LaSrMnO4±δ decorada con nanopartículas metálicas y activas de Ni incrustadas en la superficie; dicha estrategia puede ser vista como una manera muy original de mejorar las propiedades (electro)catalíticas de los materiales de ánodo y por lo tanto ser consideradas como una opción prometedora para sistemas SOFC operados con gas natural colombiano. El primer capítulo trata sobre la síntesis de la fase RP n= 2 La1.5Sr1.5Mn1.5Ni0.5O7±δ usando el método de Pechini y su caracterización. De acuerdo con la temperatura de operación de las celdas SOFC, la exsolución del Ni en atmósfera de H2 diluido a diferentes temperaturas (750, 800 y 850 °C) y tiempos de reducción fue estudiada. Las nanopartículas de Ni decorando la manganita de estructura RP n= 1 es confirmada a través de análisis de DRX, MET-EDS y el tamaño de las partículas metálicas en la superficie del óxido, inferiores a 100 nm, es caracterizado en función de las condiciones de exsolución. El segundo capítulo presenta el comportamiento catalítico del material exsuelto en la reacción de reformado de metano aplicando el concepto de reformado interno gradual (GIR por sus siglas en inglés) adaptado a celdas SOFC (en otras palabras, bajo contenido de agua, relación vapor carbono igual a 0.15) a diferentes temperaturas de reacción (750, 800 y 850 °C). Las propiedades catalíticas de las muestras impregnadas con Ni utilizando como soporte un material cerámico similar (La,Sr)2MnO4±δ, son también presentados como comparación. El material exsuelto exhibe un mejor desempeño catalítico en la reacción de reformado que la manganita impregnada, especialmente a 850 °C, mostrando una más alta conversión, velocidad de conversión y de producción de H2. Con respecto al reformado de la mezcla de alcanos ligeros (CH4 -C2H6, y CH4 -C3H8), el comportamiento catalítico es afectado debido a la competición entre moléculas y la baja disponibilidad de sitios activos metálicos, sin afectar la producción de H2. Adicionalmente, a tiempos de reacción prolongados, la actividad en el material exsuelto es estable incluso con 100 h de reacción, sin formación de especies carbonáceas sobre las partículas de Ni como lo confirman las imágenes MET y el ATG/MS. En el tercer y último capítulo, la posible formación y depósito de carbón y el envenenamiento con sulfuros son presentados. Sin embargo, a pesar de la elevada y estable actividad catalítica en la reacción de reformado de metano con vapor con una considerable resistencia a la formación de carbón, el material exsuelto tiene un alto nivel de sensibilidad al envenenamiento con H2S, similar al Ni/YSZ (material de referencia de la literatura) o al material impregnado con Ni, con una disminución de la actividad catalítica a prácticamente cero No obstante, el excepcional resultado global obtenido en el material exsuelto es prometedor para un posible uso como material de ánodo en sistemas SOFC alimentados con gas natural colombiano libre de H2S. / [CA] Molts són els problemes que han de ser resolts abans que les cel·les de combustible d'òxid sòlid (SOFC per les seues sigles en anglès) puguen operar contínuament usant combustibles hidrocarbonats com per exemple el gas natural. El risc d'una baixa activitat catalítica per al reformat del combustible, la formació i depòsit en el material d'ànode a elevades temperatures d'operació i la presència d'impureses en el combustible emprat (en particular de sulfurs) poden reduir dramàticament l'acompliment i la durabilitat de les cel·les. Tenint tot això en compte, nous materials d'ànode amb propietats (electro)catalítiques adequades són necessaris. Recentment, en el grup d'investigació INTERFASE de la Universitat Industrial de Santander (UIS), compostos de tipus manganita amb estructura Ruddlesden-Popper (RP) han sigut estudiats com a potencials materials anòdics. El seu acompliment electroquímiques ha sigut tractades en treballs previs amb resultats promissoris, però el coneixement fonamental sobre les característiques catalítiques d'aquests materials i la manera de millorar-los mitjançant l'addició de partícules metàl·liques de níquel en la superfície de l'elèctrode encara faltava. La present tesi de doctorat es va enfocar en la síntesi, caracterització i estudi d'activitat catalítica en el reformat amb vapor en condicions d'ànode de cel·les SOFC (sota contingut de vapor) d'una nova manganita de d'estructura RP (La1.5Sr1.5Mn1.5Ni0.5O7±δ), la qual, en atmosfera reductora i a elevades temperatures d'operació, promouen, a través del mecanisme de exsolució; la formació de dues fases: una manganita de composició LaSrMnO4±δ decorada amb nanopartícules metàl·liques i actives de Ni incrustades en la superfície; aquesta estratègia pot ser vista com una manera molt original de millorar les propietats (electro)catalítiques dels materials d'ànode i per tant, ser considerades com una prometedora opció per a futurs usos en sistemes SOFC alimentats amb gas natural colombià. El primer capítol tracta sobre la síntesi de la fase RP n= 2 La1.5Sr1.5Mn1.5Ni0.5O7±δ usant el mètode de Pechini i la seua caracterització. D'acord amb la temperatura d'operació de les cel·les SOFC, la exsolució del Ni en atmosfera d'H2 diluït a diferents temperatures (750, 800 i 850 °C) i temps de reducció va ser estudiada. Les nanopartícules de Ni decorant la manganita d'estructura RP n= 1 és confirmada a través d'anàlisi de DRX, MET-EDS i la grandària de les partícules metàl·liques en la superfície de l'òxid, inferiors a 100 nm, és caracteritzat en funció de les condicions de exsolució. El segon capítol presenta el comportament catalític del material d’exsolució en la reacció de reformat de metà amb vapor aplicant el concepte de reformat gradual intern (GIR per les seues sigles en anglès) adaptat a cel·les SOFC (en altres paraules, sota contingut de vapor, relació vapor-carboni de 0.15) a diferents temperatures de reacció (750, 800 i 850 °C). Les propietats catalítiques de les mostres impregnades amb Ni utilitzant com a suport un material ceràmic similar (La,Sr)2MnO4±δ, són també presentats com a comparació. El material d’exsolució exhibeix un millor resultat catalític en la reacció de reformat que la manganita impregnada, especialment a 850 °C, mostrant una més alta conversió, velocitat de conversió i de producció d'H2. En el reformat de la mescla d'alcans lleugers (CH4 -C2H6, i CH4 -C3H8), el comportament catalític és afectat per la competició entre molècules i la baixa disponibilitat de llocs actius metàl·lics, sense afectar la producció d'H2. Addicionalment, a temps de reacció llargs, l'activitat en el material d’exsolució és estable fins i tot desprès de 100 h de reacció, sense formació d'espècies carbòniques sobre les partícules de Ni, com ho confirmen les imatges MET i el ATG/MS. En el tercer i últim capítol, la possible formació i depòsit de carbó i l'enverinament amb sulfurs són presentats. No obstant això, malgrat l'elevada i estable activitat catalítica en la reacció de reformat de metà amb vapor amb una considerable resistència a la formació de carbó, el material d’exsolució té un alt nivell de sensibilitat a l'enverinament amb H2S, similar al Ni/YSZ (material de referència de la literatura) o el material impregnat amb Ni, amb una disminució de l'activitat catalítica a pràcticament zero No obstant això, l'excepcional resultat global obtingut aquest nou material és prometedor per a un possible ús futur com a material d'ànode en sistemes SOFC alimentats amb gas natural colombià lliure d'H2S. / Al Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS) por la beca de estudios de Doctorados Nacionales Conv. 647 y el proyecto # 110265842833 “Symmetrical high temperature Fuel Cell operating with Colombian natural gas”. Al Consejo Superior de Investigaciones Científicas por el apoyo con la ayuda económica para la estancia mediante la convocatoria I-coop Project # COOPA20112. / Vecino Mantilla, JS. (2020). Nickel exsolution effect on the catalytic behavior of ruddlesden-popper manganites in sofc conditions using colombian natural gas [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/149474 / TESIS
76

Study and Modeling of the Localized Nature of Top of the Line Corrosion

Singer, Marc 26 September 2013 (has links)
No description available.
77

Experimental Analysis Of The Hydrogen Sulfide Absortion Phenomena In Brine/oil Mixtures As A Function Of System Pressure And H2s

Zea, Luis 01 January 2008 (has links)
In underground oil reservoirs, Hydrogen sulfide is usually found coexisting with the oil due to bacteria reduction over a long period of time. The amount of H2S in the oil varies from place to place around the globe. When the oil extraction process begins, the presence of Hydrogen sulfide becomes noticeable as drilling tools, piping and other equipment suffer from sulfide stress cracking, electrochemical corrosion and corrosion fatigue. For this reason, the oil industry invests millions of dollars per year trying to find better ways to reduce the amount of H2S in oil. An important part of the current investigations deals with brine (sea water)/oil mixtures. The reasons are two-fold: 1) one way of extracting the petroleum from the reservoir is by injecting brine into it and since it has a higher density than oil, the latter will be ejected up to the surface. Taking into account the complex fluid flow occurring within the reservoir it is easy to understand that some brine will also be present as part of the ejected fluid; 2) brine is already present in the reservoir, so independent of the extraction method used, there will be a brine/oil mixture in the ejected flow. When brine and oil have absorbed H2S under pressure in the reservoir and then suffer a decompression during the extraction process, a certain amount of H2S is released from the liquid phase. In order to have a better prediction of how much Hydrogen sulfide can be liberated a good understanding of H2S absorption by these liquids is necessary. The amount of gas a solvent absorbs is a function of pressure, original gas concentration and temperature as described by Henry's Law. The purpose of this thesis is to experimentally analyze how much of the corrosive gas is absorbed into different brine/oil mixtures, and brine and oil, separately. In order to find sufficient data for a thorough analysis, different reservoir simulation scenarios were created. The liquids were mixed from pure brine to pure oil, resulting in 33% and 66% water cuts. Data were obtained at 2 pressures of 20atm and 70atm at room temperature. H2S concentration was also a variable, changing the original gas concentration through different values: 50, 100, and 300ppm. These experiments were conducted in an autoclave system and will better explain the hydrostatic process that occurs inside the reservoir. It was found that throughout all the water cuts, the role that total pressure plays in the absorption phenomena is of less importance as the original H2S concentration is increased. In the same manner it was observed that the highest mass-absorption ratios are always found between 50 and 100ppm and the lowest at 300ppm, this is observed for all water cuts and total pressures. Another important finding was that the ability to absorb the corrosive gas decreases as the original H2S concentration increases and this proves to be true for all water cuts and system pressures. After conducting these different reservoir scenarios, tests were conducted to simulate 300m of the horizontal section of the pipe that connects the head of the well with the platform. This was done with a high pressure 300-meter long loop. It was found that the corrosive gas is absorbed at a higher rate when there is a flow, opposite to a hydrostatic case. Henry's Law constant was identified for each water cut and each pressure, however, the test procedure could not be validated since the gas being studied was not in its pure form. Understanding the absorption phenomena of Hydrogen sulfide in different water cuts will definitely be of great help to the oil industry to make better forecasts of H2S concentrations being ejected from each well.
78

Regulating the Biomedical and Biocatalytic Properties of Amphiphilic Self-assembling Peptides via Supramolecular Nanostructures

Li, Zhao 28 August 2023 (has links)
Self-assembly is a fundamental process in the field of nanotechnology, where molecules organize into complex structures spontaneously or induced by environmental factors. Peptides, short chains of amino acids, can self-assemble into many types of nanostructures. The self-assembly of peptides is governed by noncovalent interactions, including electrostatic interactions, hydrogen bonding, hydrophobic interactions, aromatic-aromatic interactions, and van der Waals forces. By varying the amino acid sequences and manipulating environmental parameters, these interactions can be modulated to obtain diverse supramolecular nanostructures, exhibiting a wide range of physical, chemical, and biological properties. Furthermore, the ability to control these properties opens up a world of possibilities in biomedical and biocatalytic applications. From drug delivery systems to enzyme mimics, as well as cancer treatments, the potential of these self-assembling peptides is vast and continues to be a vibrant area of research. Exploiting this potential, this dissertation delves into the design, synthesis, and investigation of self-assembling peptides for a range of applications. The introductory chapters of this document lay the groundwork, providing a comprehensive overview of self-assembly and its potential in biocatalytic and biomedical domains. The focus shifts in the later chapters to drug delivery applications, particularly in the delivery of hydrogen sulfide (H2S), and its implications in cardioprotection and cancer treatment. Finally, this document details an evaluation of self-assembled peptides in the context of biocatalysis using a combined experimental and computational approach. Chapter 3 discusses the design and synthesis of peptide-H2S donor conjugates (PHDCs) with an unusual adamantyl group. Several of PHDCs studied in this chapter self-assembled into novel nanocrescent structures observed under both conventional transmission microscopy (TEM) and cryogenic TEM (cryo-TEM). By varying the C-terminal amino acid with cationic, nonionic, or anionic amino acids, the PHDC morphologies remained unaffected, offering a robust peptide design for crescent-shaped supramolecular nanostructures. Chapter 4 discusses an extension of this project, introducing a cyclohexane in PHDCs instead of an adamantyl group. In this work, we designed and fabricated four constitutional isomeric PHDCs, which self-assembled into nanoribbons with different dimensions and large nanobelts. These morphologies exhibited varying cellular uptake and in vitro H2S release amounts, influencing their protective effects against oxidative stress induced by H2O2. With the knowledge of the impact of subtle changes in PHDC structures, Chapter 5 discusses our further design of three more PHDCs with the variation of side chain capping group, from an aromatic phenyl ring to a cyclohexane unit, to an aliphatic n-hexyl chain. In this chapter, we studied how changes in the hydrocarbon tail can influence the supramolecular nanostructures and their potential ability for colon cancer treatment. A final aspect of H2S delivery in Chapter 6 involves the creation of a stable PHDC with an extended H2S release profile. By integrating the H2S donor into a β-sheet forming peptide sequence with a Newkome-like poly(ethylene glycol) dendron, this PHDC self-assembles into spherical or fibril nanostructures with or without stirring. The H2S release was further studied by triggering release with various charged thiol molecules. Finally, another facet of this document focuses on three constitutional isomeric tetrapeptides containing a catalytic functional amino acid, His. Chapter 7 discusses these tetrapeptides, which self-assembled into nanocoils, nanotoroids, and nanoribbons based on the position of the His residue in the peptide sequence. Computational studies simulating the self-assembling process revealed the distribution of His residues and hydrophobic pockets, reminiscent of natural enzyme binding sites. A tight spatial distribution of His residues and hydrophobic pocket in nanocoils provided a picture for why this morphology exhibited the highest rate enhancement in catalyzing a model ester hydrolysis reaction. This study demonstrated how subtle molecular-level changes impact supramolecular nanostructures and catalytic efficiency. The final chapter details conclusions on all the research in this dissertation and discusses further directions of self-assembling peptides in the application of drug delivery and design of catalyst mimics. / Doctor of Philosophy / Self-assembly is a fascinating process in nanotechnology, where molecular building blocks come together to form complex structures. Peptides, which are short chains made up of amino acids, can play a crucial role in this process. They can organize themselves into various shapes due to different forces acting between their amino acid building blocks. By changing the arrangement of amino acids and adjusting the environment, scientists can create a wide range of nanoscale structures with unique properties from peptides. These self-assembling peptides have enormous potential in fields like medicine and catalysis. This dissertation describes how to design and make self-assembling peptides for various uses. Chapter 1 describes the general structure of the document, and Chapter 2 discusses the basics of self-assembly and how it can be applied in medicine and other areas. Chapters 3-6 focus on using self-assembling peptides to deliver hydrogen sulfide (H2S), a noxious gaseous molecule that is now recognized as a vital signaling molecule involved in various physiological processes. Several classes of peptide-H2S donor conjugates (PHDCs) are discussed in these chapters, including PHDCs that form nanoscale crescents, twisted ribbons, fibers, and other structures. These nanostructures show promise in protecting cells from harmful substances or can act as drugs in cancer treatment. We also investigate how different modifications affect their performance in biomedical applications. The final research chapter, Chapter 7, involves using self-assembling peptides as catalysts, molecules that speed up chemical reactions. By arranging the amino acids in different ways, peptides that form nanoscale coils, toroids, or ribbons-like structures were created. These different shapes influenced how well they catalyzed reactions. Computational modeling studies helped explain how small differences in molecular design led to big impacts on their catalytic abilities. The final chapter discloses conclusions on all the research in this dissertation and discusses the further directions of self-assembling peptides as medicines and catalysts.
79

Investigation of Localized Corrosion of Carbon Steel in H2S Environments

Fang, Haitao January 2011 (has links)
No description available.
80

Stimuli-Responsive Peptide-Based Biomaterials: Design, Synthesis, and Applications

Zhu, Yumeng 15 May 2023 (has links)
Peptide-based biomaterials have gained much interest in various applications in drug delivery and tissue engineering in recent years, in large part due to their typically excellent biocompatibility and biodegradability. Composed of different amino acids, peptides can be designed with numerous sequences, providing flexibility and tunability in biomaterials. Peptides are easy to modify with small molecule drugs, inorganic components, and polymer chains to access multiple functions and tune properties relevant to biology and medicine. Stimuli-responsive peptide-based biomaterials can respond to environmental stimuli, such as light and ultrasound, in addition to local environmental factors, such as temperature, enzyme activity, and pH. Under environmental changes, these materials can be triggered to release therapeutic payloads, change conformations, or induce self-assembly in the target sites. In this work, I introduce the design, synthesis, and potential applications of several stimuli-responsive peptide-based biomaterials. The first half of this dissertation is based on enzyme-responsive, peptide-based biomaterials as extracellular matrix (ECM) mimics in tissue engineering. We synthesized linear and dendritic elastin-like peptides (ELPs) as crosslinkers and conjugated them with hyaluronic acid (HA) to form hydrogels. Trypsin was used as the enzyme trigger for cleaving the C-terminal lysine and to study how crosslinker topology affects enzymatic degradation. Hydrogels with dendritic ELPs degraded more slowly than linear ELPs, providing a novel strategy to tune the degradation rate of hydrogels as ECM mimics by the molecular design of crosslinker topology. Building on this peptide-polysaccharide platform for synthetic ECM design, we subsequently prepared hydrogels embedded with bioactive cryptic sites. These novel polymeric hydrogels mimicked native ECM cryptic sites by using depsipeptides that undergo an enzyme-triggered molecular rearrangement, "switching" from a non-functional epitope to a bioactive sequence. Mass spectrometry, 1H and 13C NMR spectroscopy, and fluorescence studies were applied to track structural changes in the peptide. SEM was used to image these polymer-peptide hybrid hydrogels. Finally, in vitro studies were conducted to evaluate cell interactions with the hydrogels. Switch peptide-modified alginate hydrogels showed increased cell adhesion upon induction of enzymatic activity, which provided a "gain of function" of the synthetic ECM. Critically, enzymes associated with the cells themselves could trigger the peptide switch and change in synthetic ECM behavior. With knowledge of stimuli-responsive peptide-based biomaterials applied in tissue engineering, I then studied how this system could be used in drug delivery by designing peptide-hydrogen sulfide (H2S) donor conjugates (PHDCs). H2S is a gasotransmitter that is produced endogenously, which has been explored in recent years with many potential therapeutical applications. We studied H2S release profiles in dual-enzyme-responsive PHDCs, with a further investigation into PHDC–Fe2+ complexes for potential tumor treatments via chemodynamic therapy. The PHDC–Fe2+ complexes were examined in a C6 glioma cell line, exhibiting an improved cell-killing effect compared with controls, by inducing toxic hydroxyl radical generation (•OH) via a Fenton reaction. To this end, we further discovered how side chains influence self-assembling nanostructures, H2S release profiles, and biological activities via three constitutionally isomeric PHDCs. Different morphologies and varied H2S release rates were observed, paving the way for tuning the properties of PHDCs by simple changes in molecular design. Finally, this dissertation discloses conclusions and future directions on stimuli-responsive peptide-based biomaterials using similar platforms with different designs in the drug delivery and tissue engineering fields. / Doctor of Philosophy / Peptides, short sequences of two or more amino acids linked by chemical bonds, are smaller versions of proteins. Forming naturally in nature, peptides are promising candidates in the design of biocompatible and biodegradable materials. To make these peptide-based materials "smart", certain sequences or functional groups are installed in the peptides, making them responsive to environmental changes, or stimuli. These external stimuli include light, ultrasound, temperature, enzyme activity, and pH changes. In this work, we have explored the design and synthesis of stimuli-responsive peptide-based biomaterials and their potential applications in tissue engineering and drug delivery. The first half of this dissertation focuses on the design and synthesis of two enzyme-responsive, peptide-based materials that function as extracellular matrix (ECM) mimics. The ECM is a three-dimensional microenvironment where cells reside, providing structural support and adhesive anchor points for cells. In the first system, we synthesized peptide-polysaccharide hydrogels with different peptide crosslinkers, comparing their enzymatic degradation performance to evaluate how peptide topology (architecture) influences degradation. A more branched topology led to a slower hydrogel degradation rate. To introduce biofunctionality into the ECM mimics, we embedded the second system with a "switchable" peptide sequence, which transformed from a non-functional peptide into a functional, bioactive epitope after being triggered by an enzyme. The functional peptide after the switch provided cell adhesion and increased cell spreading. The latter half of this dissertation explores the possibility of stimuli-responsive peptide-based biomaterials in drug delivery. We designed peptides that release hydrogen sulfide (H2S), a signaling gas is commonly known for its foul smell and toxicity, and studied the biological behaviors in cells. The peptide-H2S donor conjugates (PHDCs) were activated by the enzyme legumain, which cancer cells overproduce, leading to H2S release. With the combined treatment with Fe2+, the PHDC-Fe2+ system reduced cancer cell viability due to the high amount of hydroxyl radicals (•OH) generated by the Fenton reaction. This system may be a potential design platform for precise tumor treatments.

Page generated in 0.1045 seconds