No description available.

Clay-based compounds are globally the most advanced and widely used method of direct suppression of marine harmful algal blooms, and are currently undergoing investigation as an option to control Karenia brevis blooms in Florida. Before clay may be accepted for widespread use, there are multiple concerns and challenges that must be addressed regarding the environmental safety of this method, such as effects on water quality, the fate of toxins, and potential impacts of clay treatment to non-target organisms. To contribute to ongoing assessments of clay as a potential control method for K. brevis blooms, we conducted experiments with a formulation of kaolinite clay modified with polyaluminum chloride known as Modified Clay II (MC II). In these experiments, we evaluated water chemistry dynamics and physiological responses in several bottom-dwelling marine species with ecological and economic significance, including blue crab (Callinectes sapidus), sea urchin (Lytechinus variegatus), and hard clam (Mercenaria campechiensis). First, we conducted an experiment with blue crabs in 20 L aquarium tanks (N = 48), exposing the animals to cultured K. brevis (1 x 106 cells L-1) and MC II (0.5 g L-1) and measuring mortality and reflexes over 192 hours. In our second experiment, K. brevis (1x106 cells L-1) and MC II (0.2 g L-1) were applied to 1,400 L mesocosms (N = 9) containing blue crabs, sea urchins, and hard clams, which were observed over 96 hours. In our final experiment, we modified the methods of the previous experiment to again examine K. brevis (1x106 cells L-1) and MC II (0.2 g L-1) with the same model species in 1,400 L mesocosms (N = 12) over 72 hours. In these two mesocosm experiments, we observed cell and toxin removal, changes in water quality characteristics including nutrients and carbonate chemistry, and measured mortality, respiration rate, reflexes, and internal toxin content. Our results were congruent across our three experiments. Treatment with MC II significantly reduced cell concentrations but did not reduce toxin concentrations in the water column. We found no notable impacts of clay treatment to reflexes, respiration rates, or internal toxin content for either of our three species. No significant differences in mortality were found for our three species, excluding crabs in the first mesocosm study, which were found to have pre-existing infections that confounded our results. Analyses of nutrients indicates MC II may remove dissolved phosphorus from the water column, and the potential to improve water quality which may make this formulation of clay desirable to managers. Overall, treatment with this formulation of clay did not appear to induce any significant measured effects on the model species within the observed time frames of these experiments. Clay appears to be a promising option to treat K. brevis blooms given its low cost, ease of application, and negligible impacts to the environment, and its use may relieve the damaging effects of K. brevis blooms by preventing mortalities that would otherwise occur were blooms allowed to persist. We therefore recommend that clays, including MC II, be considered for additional laboratory and field tests, with the goal of obtaining further information on potential ecological impacts so that managers and researchers can make informed decisions on the use of bloom control technologies in Florida waters.

No description available.

La composition des communautés de protistes et leur dynamique temporelle sont traditionnellement étudiées en analysant des séries de données de surveillance/observation dont la mise en place est relativement récente (≤40 ans). Dans cette étude, nous avons analysé les traces biologiques (formes de résistance et ADN ancien) préservés dans des sédiments couvrant une échelle temporelle d’environ 150 ans afin d’étudier les changements de la composition et la dynamique temporelle des protistes marins, principalement dans deux écosystèmes estuariens de la rade de Brest (Bretagne, France). Les analyses de metabarcoding montrent que seulement une partie minoritaire (16-18%) de la richesse des protistes (#OTUs) des sédiments superficiels est retrouvée dans les sédiments profonds et que la plupart des protistes présents dans les sédiments anciens sont connus pour être capables de produire des formes de résistance. La composition des communautés de protistes étaient différenciées en deux principales paléocommunautés (avant/après 1950), suggérant une biodiversité spécifique à chaque période. Les abondances relatives des dinoflagellés ont montré une tendance à la baisse depuis les années 70’ et les genres Alexandrium et Gonyaulax ont montré une dynamique opposée en termes d’abondance relative à travers le temps. Les données paléogénétiques (PCR en temps réel) suggèrent qu’A. minutum est présente dans la rade depuis au moins 1873 ± 7 et qu’au cours des derniers 150 ans, l’espèce est devenue envahissante, proliférant dans la rade seulement ces dernières années. De plus, Les données de PCR en temps réel suggèrent que la partie sud-est de la rade, où des sédiments à typologie vaseuse sont plus abondants, est potentiellement plus favorable à l’accumulation des kystes de l’espèce. Les analyses écophysiologiques (taux de croissance, taux de consommation de phosphore, biomasse maximale atteinte) réalisés sur des souches de dinoflagellés (A. minutum et Scrippsiella donghaienis) obtenues par germination montrent une forte variabilité phénotypique intraspécifique au sein des deux espèces et dans les deux milieux étudiés. Les résultats de ces travaux de thèse contribuent au domaine de la recherche en paléoécologie sédimentaire, montrant les avantages et les limites de cette approche pour révéler des patrons biologiques encore peu explorés. / The community composition of protist and their temporal dynamic are are traditionally studied by analyzing data sets of monitoring/observation networks, whose implementation is however relatively recent (≤40 years). In this study, we analyzed the biological traces (resting stages and ancient DNA) preserved in sediments covering a time scale of 150 years in order to study changes in the composition and the temporal dynamics of marine protists, focusing mainly on two estuarine ecosystems of the Bay of Brest (Brittany, France). Metabarcoding analyses showed that only a minor part (16-18%) of the protists richness (#OTUs) of superficial sediments is retrieved in deep sediments, and that most of the protists found in ancient sediments are known to produce resting stages. Two main paleocommunities were differentiated (before/after 1950), suggesting the existence of a distinct and specific biodiversity for the identified periods. The relative abundances of dinoflagellates showed a decreasing trend since the 70s' and Alexandrium and Gonyaulax genera showed an opposite dynamic in terms of relative abundance over the time. Paleogenetic data (real-time PCR) suggest that A. minutum is present in the Bay of Brest since at least 1873 ± 7 and that, across a time scale of about 150 years, the species has proliferated only recently in the estuaries of the bay. Moreover, real-time PCR data suggest that the south-eastern part of the bay, where muddy sediment are more abundant, is potentially more favorable for the accumulation of the species cysts. Ecophysiological analyses (growth rate, phosphorus assimilation rate, and maximal biomass attained) performed on dinoflagellate strains (A. minutum and Scrippsiella donghaienis) showed a strong phenotypic intraspecific variability for both species and for both analyzed media. The results of this thesis work contribute to the research in sedimentary paleoecology, showing the advantages and limits of this approach to reveal still underexplored biological patterns.

Les proliférations de microalgues peuvent être nocives. L'augmentation récente de la fréquence et de l’étendue géographique des efflorescences de dinoflagellés benthiques toxiques comme Ostreopsis cf. ovata peut poser de réels problèmes de santé publique. La côte méditerranéenne Nord-Occidentale (lieu de cette étude) est l'une des nombreuses régions méditerranéennes où les proliférations d'algues nuisibles représentent une menace pour l’économie touristique. Dans les années à venir, une attention particulière devra être portée à la gestion et la prévision des proliférations de ces microalgues nuisibles benthiques et c'est dans ce contexte que cette étude a été menée. Une étude bibliographique portant sur les proliférations d’Ostreopsis spp. indique un manque important de données en relation avec l'écologie d’O. cf. ovata, en particulier concernant les substrats biotiques, ainsi que les communautés les habitats benthiques et, par conséquent, les rôles éventuels que ceux-ci peuvent jouer en tant que stimulateurs majeurs des efflorescences d’Ostreopsis spp. Durant les étés 2015 et 2016, des expériences in situ de courte durée ont été menées sur les récifs côtiers et ont été complétées par des expériences en laboratoire. Les résultats obtenus ont permis d’établir que quatre communautés distinctes de macroalgues abritent des abondances différentes d’O. cf. ovata. En particulier, les abondances les plus élevées ont été enregistrées dans des sites dominés par des structures communautaires peu complexes ; Turf et Dictyotales, alors que les sites dominés par des communautés complexes de Cystoseira n’ont montré aucune prolifération significative de microalgues. Ces résultats impliquent que les régions côtières dominées par les communautés composées de Cystoseira spp. pourraient potentiellement réduire les proliférations de Ostreopsis spp. En outre, d’autres facteurs abiotiques, tels que les concentrations en nutriments et métaux traces, ne contribuent pas (ou peu) à expliquer la dynamique des populations de O. cf. ovata. Les multiples facteurs de stress d’origine anthropique continueront à influencer le fonctionnement de l’écosystème marin. La compréhension de ces impacts et la façon dont ils influencent la dynamique des dinoflagellés benthiques toxiques est impérative pour prévoir, gérer et éventuellement réduire ces proliférations, à l’échelle de l’océan mondial. / Algal blooms can be harmful. The global management and forecasting of benthic harmful algal blooms (BHABs) will be of increasing importance in the years ahead and that is what this study sets out to address. The increase over recent decades, in both frequency and geographical range, of the potentially harmful benthic dinoflagellate Ostreopsis cf. ovata can pose real problems for human health. The French Côte d’Azur, the location for this study, is just one of many Mediterranean areas where harmful algal blooms pose a potential economic threat to a tourist-based economy. A review of the existing literature on Ostreopsis spp. blooms shows a severe lack of information about the ecology of O. cf. ovata in relation to biotic substrates, communities and habitats and thus any possible roles these may play in fostering major Ostreopsis spp. blooms. Through a series of in situ experiments on temperate reefs on the Côte d’Azur over the summers of 2015 and 2016 with follow-up experiments in the laboratory, this study establishes that four distinct macroalgal communities harbour different O. cf. ovata abundances. The results indicated that higher abundances were recorded in sites which were dominated by less complex community structures; Turf and Dicyotales, while sites with Cystoseira spp. communities present did not harbour significant microalgal blooms. These results imply that coastal regions with a dominance of Cystoseira-composed-communities could potentially be less prone to blooms or even inhibit Ostreopsis spp. proliferation. Although, no clear relationship was found between inorganic nutrient concentrations and O. cf. ovata abundances, it was observed that in the bloom onset period, nitrogen compounds were higher than in the rest of the study period (both in 2015 and 2016). Multiple human stressors will continue to impact marine vegetation, understanding these impacts and how they then influence bloom dynamics is imperative for the global management and mitigation of BHABs.

No description available.

The St. Johns River (SJR; Jacksonville, FL, USA) is a large, brackish, estuarine system characterized by considerable anthropogenic pollution, recurrent harmful algal blooms (HABs), and diverse toxin-producing cyanobacteria. The most prevalent toxins in SJR water samples are microcystins/nodularins (MCs/NODs). Additionally, the SJR provides critical habitat for a genetically and behaviorally distinct estuarine community of bottlenose dolphins (Tursiops truncatus) that routinely uses and strands in low mesohaline and oligohaline areas of the river. This population has been subject to two unusual mortality events (UME) since 2010 and has since been described as having substantial declines in population health, characterized by widespread dermatitis and emaciation. Additionally, three dolphins have been recovered from low salinity habitats with epidermal algal mats. Because dolphin illness and strandings overlapped temporally and spatially with confirmed cyanobacterial blooms in the SJR, there is concern that estuarine dolphin health may be declining due to exposure to toxic cyanobacteria and HAB events. Specific to this study, the SJR estuarine community was considered a high-risk group for cyanotoxin exposure in relation to coastal animals. This study analyzed all available hepatic tissues for estuarine dolphins, and used samples from coastal individuals that stranded outside of the known cyanotoxin bloom season as controls. Three analytical methods were used to determine MCs/NODs presence in dolphin liver and epidermal algal mat samples. An Adda ELISA and LC-MS/MS were used to determine free MCs/NODs presence while the MMPB technique was used to determine total (bound and free) concentrations and as confirmatory analyses. ELISA analyses produced high values that were not supported by concurrent LC-MS/MS or MMPB analyses, indicative of false positives. MMPB testing resulted in low-level total MCs/NODs detection in some specimens. Results indicate that both estuarine and coastal dolphins are exposed to MCs/NODs, with potential toxic and immune health impacts.

No description available.

No description available.

Le waqf est l'un des phénomènes socioculturels religieux majeurs du monde musulman, en vigueur dans les sociétés islamiques depuis les débuts de l'islam jusqu'à nos jours. Dans cette recherche, on s'est attaché à l'étude du waqf selon l'école chiite imâmite duodécimaine en Iran du 16ème au 18ème siècle. Pour que cette analyse soit la plus complète possible, on a abordé la question sous trois aspects complémentaires. Ainsi, la première partie examine le waqf dans les ḥadîths de la tradition chiite duodécimaine, les présentant, les traduisant et les analysant alors que la seconde partie se concentre sur des positions des jurisconsultes les plus influents de la période concernée par rapport à la praxis et à la théorie de waqf. La recherche sur les pratiques de waqf dans la société safavide compose la troisième partie de cette thèse. Cette approche tripartite a produit une étude qui analyse les fondements théoriques de waqf dans le chiisme duodécimain ainsi que des pratiques mêmes de waqf en islam chiite. Cette recherche constitue une première étape pour comprendre la spécificité du waqf chiite dans un cadre géographique et historique précis qui façonna les bases de la jurisprudence chiite contemporaine sur le waqf qui reste toujours d'actualité. / Waqf is one of the major sociocultural phenomena in Islam since the early generations up until today.This dissertation focuses on waqf according to the Twelver Imamite Shiite doctrine in Iran from the 16th to the 18th century. To study this in different ways, we have used three complemetary aspects. The first part closely examines waqf in Twelver Shiite ḥadîths, presenting, translating and analyzing them while the second section concentrates on the positions of the most influential Shiite ulama of the period with respect to waqf theory and praxis. Research on waqf practices in Safavid society make up the third section of this thesis. This tripartite approach has produced a study which analyzes the theoretical foundations of waqf in Twelver Shiism as well as the practices of waqf in Shiite Islam. This research is a step on the way to understanding the specificity of Shiite waqf within a geographical and historical context which has established the basis for contemporary Shiite jurisprudence on waqf up until today.