Thesis--Karolinska Institutet. / Extra t.p. with thesis statement inserted. Four articles by the author reprinted from Acta odont. Scand. and Sc. ed. bull. in pocket. Includes bibliographical references (p. 31-34).

A time-resolved holographic interferometer specially suited for high-speed visualization of the gas flow in shock tube experiments has been developed. Holographic interferometry, which is based on the recording of two coincident holograms at different times so that one of them acts as a reference field, can accurately reveal the density distribution in a gas. The device described here fills the need for a practical method to record short sequences of holographic interferograms documenting the evolution of shock wave reflections that are not self-similar in time. Multiple hologram recording was implemented on an existing holographic interferometric system through the technique of spatial frequency multiplexing, in which the holograms are overlaid but the reference beam is angled differently for each exposure. Because the object beam is not involved in the multiplexing process, the imaging optics of the original system could be left unmodified. The upgrade only entailed the introduction of an angular sweeping system in the reference beam path. The beam multiplexing assembly was initially based on a spinning mirror design, which produced fairly satisfactory recordings of non-interferometric holographic sequences but was incapable of accurately overlaying a second set of exposures establishing the reference field for each image. The mechanical sweeping system had other drawbacks as well, among them the tendency to create extraneous fringes in the holographic images because of the unavoidable angular motion of the reference beam over the duration of a laser pulse. A solid-state multiplexing system was then devised in which the reference beam was split into several branches, each aimed at the film from a different direction and individually shuttered by a ferroelectric liquid crystal light valve. Beam sweeping was achieved by opening the shutters in sequence as the laser was pulsed, but it was also possible to record the reference exposure on all images simultaneously with a single laser pulse by having all shutters open at the same time. A prototype three-image system was constructed and successfully tested by recording interferometric sequences of a shock wave reflecting off a model at framing intervals down to 100 μs. / Graduate

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The main aim of this study is to extending the holographic interferometry technique to measure the out-of-plane displacement of an object subjected to both temperature and displacement field. It is noted that both the out-of-plane displacement and the ambient temperature change can cause image fringes. Therefore, an auxiliary object is used to identify the fringe numbers caused by the ambient temperature change during the experiment. The warpage measurement of a PBGA package is used as an example. It can be shown that the proposed method works

No description available.

No description available.

Apesar dos pesquisadores afirmarem que os materiais odontológicos restauradores, devido às suas diferentes propriedades mecânicas, influenciam na transmissão da carga para os tecidos de suporte, não foi encontrada comprovação científica concludente. Os trabalhos de análise da distribuição da carga oclusal com diferentes materiais de reconstrução protética não são concordantes em seus resultados. Assim, diante da importância da seleção de materiais restauradores protéticos no prognóstico da reabilitação oral, este trabalho se propôs a estudar a influência de coroas protéticas metalo-cerâmica e de resina, cimentadas no canino, na transmissão de tensões para o tecido ósseo em mandíbulas frescas de cães, utilizando o método de interferometria holográfica de dupla exposição. Seis hemi-mandíbulas frescas de cães da espécie Canis familiares foram fixadas em um dispositivo para garantir a invariabilidade da força aplicada durante o experimento. Uma carga de 0,98 N foi aplicada sobre as coroas, seguindo o longo eixo do dente. Pelo método de interferometria holográfica, foram obtidos 12 hologramas, 6 para cada grupo de amostras. As coroas metalo-cerâmicas apresentaram maior deslocamento, com movimento de intrusão no alvéolo, em relação às de resina. Os microdeslocamentos resultantes nas hemimandíbulas demonstraram comportamentos semelhantes de deflexão, porém, não foram encontradas diferenças estatisticamente significantes na distribuição das tensões ao osso, com relação aos dois tipos de coroas consideradas, demonstrando a influência da presença do ligamento periodontal. / Although researches assert that restoring dental material, due to their different mechanical properties, influences at load transmission to support tissues, it was not find any conclusive scientific evidence. Researches with finite element, implants and different prosthetic reconstruction material do not have an agreement in terms of results. So, considering the importance of prosthetic restoring material selection on oral rehabilitate prognosis, this assignment studied the influence of metaloceramic and resin prosthetics crowns, cemented on canine, during stress transmission to bony tissue of post-mortem dog mandibles, using double exposure holographic interferometry method. Three fresh mandibles were split into 6 hemi-mandibles, which were fixed in a proper device in order to assure the same applied force during the whole experiment. A 0,98 N load was applied to the crowns, following the long axis of the tooth. It was collect 12 holograms, 6 for each sample group. Metaloceramic crowns presented larger intrusion micromovement in the alveolus, comparing that of resin ones. The resulting micromovings in the hemimandibles presented similar deflecting behaviour. However, significant statistical differences on bone tension distribution have not been found, concerning the two types of previously referred crowns, demonstrating the influence of periodontal ligament.

A interferometria holográfica é um poderoso método óptico para observação de fenômenos perturbativos e na análise de superfícies, sendo extremamente útil em aplicações em pesquisa básica, biomédicas e tecnológicas. Os cristais fotorefrativos se apresentam como um meio atrativo para registro de hologramas. O fenômeno que caracteriza esses cristais é o efeito fotorrefrativo. Tal efeito consiste na modulação do índice de refração através de fotoindução e efeito eletro-óptico linear (Efeito Pockels), o que permite o registro de hologramas de fase em volume. Entre as características que recomendam os cristais fotorrefrativos para interferometria holográfica podemos citar: o processo de registro e leitura ocorre de maneira dinâmica através da técnica de mistura de duas ondas; o fato de serem meios revers´?veis dispensando processamento químico e a boa resolução implicando imagens holográficas de boa qualidade. Analisamos as diversas tensões que ocorrem no processo mastigatório, estudando principalmente a concentração de cargas e as dissipações nesse processo, utilizando a técnica holográfica de dupla exposição e o cristal fotorrefrativo Bi12SiO20 (BSO), da família das Silenitas, como meio de registro. O estudo em crânio seco e a simulação dos principais feixes dos músculos da mastigação permitem realizar a contração dos mesmos, com aproximação melhor que os métodos de elementos finitos e resinas fotoelásticas, em estruturas anisotrópicas como o crânio. / The holographic interferometry is a powerful optical method for observation of the disturbance phenomena and in the analysis of surface, being extremely useful in application in basic research, biomedical and technological. The photorefractive crystals present as an attractive holographic recording medium. The phenomenon that characterize these crystals is the photorefractive effect. Such effect consistis of the index of refraction modulation grating via the linear eletro-optic effect (the Pockels effect) and photoindution, that it allow phase holographic recording in volume. Among the characteristics that admit the photorefractive crystals for holographic interferometry we can cite: recording and reading tecniques to occur in dynamics way through the tecnique of two-wave mixing, it is reversible medium resulting holographic images of good quality. We analyze the varied strains that occur in the masticatory process, mainly studying the load concentration and the dissipation in this process, using the double-exposure holographic technique and the photorefractive crystals Bi12SiO20 (BSO), of the sillenite family, as holographic recording medium. The study in dry skull and the simulation of main beams of the mastication muscles allow to realize the concentration of the same ones, with better approach than the methods of finite elements and photoelastics resins, in anisotropic structures as the skull.

The middle ear plays an integral role in the normal hearing process by transforming sound energy from the air inside the ear canal into vibrations of the inner-ear fluid, and a malfunction in any middle-ear component can lead to significant hearing loss. Despite decades of research on the Tympanic Membrane (TM or eardrum), the transformation of sound energy into ossicular mechanical vibrations is not yet well understood. Part of this is because the available clinical and research tools provide insufficient data to understand the complexities of this transformation. The data insufficiency arises due to methodological, technological, and physiological limitations such as required nanometer and microsecond spatio-temporal resolutions of the sound-induced TM motions. Although holographic methods provide nondestructive non-contact measuring capabilities that satisfy most of the constraints for TM measurements, the influence of large submillimeter scale physiological motions in live samples produced by heartbeat and breathing can result in near complete saturation of TM holograms. In this Dissertation, a new high-speed correlation interferometry holographic method is proposed that can compensate for the effects of physiological noise using an open-loop control configuration. Preliminary animal measurements with the proposed method demonstrate the necessary accuracy and precision to measure the motion of the entire TM produced by short- duration (≥1 kHz) transient stimuli. Such rapid measurements reduce the effect of the longer and slower environmental and physiologic noises, and enable clinical applications. In the second part of this Dissertation, a novel multiple wavelength high-speed holographic interferometric shape measurement method is incorporated into the high-speed displacement measurements. The method uses the imaging optics of the displacement measurement system to perform shape and orientation measurements. Displacement and shape measurements can be made in less than 200 msec and allow computation of true surface-normal displacements. The surface-normal measurements are independent of the direction of observation, which helps comparisons of measurements made after changes in TM orientation or location. The results enable accurate and precise shape and displacement measurements for use in applications such as modal and finite element analyses, additive manufacturing of prosthetic TM grafts, clinical diagnosis, hearing rehabilitation, as well as optimization of hearing devices. In addition, measured shape parameters such as curvature, depth of cone etc., can help us understand TM mechanics and contribute to quantitative diagnostic assessments.

This dissertation presents techniques of three-dimensional microscopy. First, an economical method of microscopic image formation that employs a raster-scanning laser beam focused on a sample, while non-imaging detector receives the scattered light is presented. The images produced by this method are analogous to the scanning electron microscopy with visible effects of shadowing and reflection. Compared to a conventional wide-field imaging system, the system allows for a greater flexibility, as the variety of optical detectors, such as PMT and position-sensitive quadrant photodiode can be used to acquire images. The system demonstrates a simple, low-cost method of achieving the resolution on the order of a micron. A further gain in terms of resolution and the depth of focus by using Bessel rather than Gaussian beams is discussed. Then, a phase-imaging technique to quantitatively study the three-dimensional structure of reflective and transmissive microscopic samples is presented. The method, based on the simultaneous dual-wavelength digital holography, allows for higher axial range at which the unambiguous phase imaging can be performed. The technique is capable of nanometer axial resolution. The noise level, which increases as a result of using two wavelengths, is then reduced to the level of a single wavelength. The method compares favorably to software unwrapping, as the technique does not produce non-existent phase steps. Curvature mismatch between the reference and object beams is numerically compensated. The 3D images of porous coal samples and SKOV-3 ovarian cancer cells are presented.