Apresentaremos alguns resultados de rigidez e de bifurcação para soluções do problema de Yamabe em variedades produto com bordo. / We will discuss some rigidity and bifurcation results for solutions of the Yamabe problem in product manifolds with boundary.

Nowadays, autonomous driving revolution is getting closer to reality. To achieve the Autonomous driving the first step is to develop the Advanced Driver Assistance System (ADAS). Driver-assistance systems are one of the fastest-growing segments in automotive electronics since already there are many forms of ADAS available. To investigate state of art of development of ADAS towards Autonomous Driving, we develop Systematic Mapping Study (SMS). SMS methodology is used to collect, classify, and analyze the relevant publications. A classification is introduced based on the developments carried out in ADAS towards Autonomous driving. According to SMS methodology, we identified 894 relevant publications about ADAS and its developmental journey toward Autonomous Driving completed from 2012 to 2016. We classify the area of our research under three classifications: technical classifications, research types and research contributions. The related publications are classified under thirty-three technical classifications. This thesis sheds light on a better understanding of the achievements and shortcomings in this area. By evaluating collected results, we answer our seven research questions. The result specifies that most of the publications belong to the Models and Solution Proposal from the research type and contribution. The least number of the publications belong to the Automated…Autonomous driving from the technical classification which indicated the lack of publications in this area.

"Intelligent Tutoring Systems (ITSs), while effective in enhancing students’ problem solving skills, are difficult and time-consuming to build. In order to reduce the length and the complexity of ITS construction, authoring tools are used. These tools provide a solid foundation for creating pedagogical exercises for students, and offer graphical user interfaces that eliminate the need for programming expertise. One of the major problems with today’s authoring tools is that they are still quite intricate and time-consuming to utilize, even for users who are familiar with them. Their steep learning curves often intimidate users who are only interested in creating simple tutoring systems. I have designed and implemented an authoring tool, called Mason, which strips away the visual interface design features of today’s top ITSs, and focuses on the creation of sophisticated pedagogical exercises using a hierarchical domain model. The exercise creation process includes the definition of numerous components, such as: a problem statement, the desired answer to the exercise, the strategies for tutoring students on the mistakes they make while trying to formulate the correct answer, and diagnostic rules for launching the appropriate strategies for specific student errors. The ultimate goal of Mason is to be able to significantly reduce the time needed to author text-based ITSs that are able to diagnose student answers and generate pedagogical dialogue accordingly. This goal was verified by using Mason to replicate the architecture of Ms. Lindquist, a sophisticated ITS for algebra that originally took over a year a construct. The replica was finished in less than a week, and was able to emulate Ms. Lindquist’s dialogue generation accurately with minor limitations."

Made available in DSpace on 2015-04-22T22:11:28Z (GMT). No. of bitstreams: 1 Ana Paula.pdf: 562305 bytes, checksum: 4d45fcaefda037e6577f067eeca7c25e (MD5) Previous issue date: 2007-12-21 / Global connections have contributed to expand the companies horizons improving the relationship between clients and suppliers. The survival in a competitive world demands a constant exercise of establishment objectives and goals defined in the strategic planning that shall be shared by the whole organization and monitored constantly. In this environment, the decision to use an integrated system and sophisticated internal controls related to financial management and its tools in order to provide reliable information preferably in real time are seen as competitive advantages, essential to speed up the decision process. The aim of this work is to analyze aspects of the strategic and financial management, their integration and the connections established between them. References to tools and information systems used by the financial management are made and the extension they add value to strategic management is also analyzed. As materials and methods, a case study has taken place in a company that produces electro electronic parts located in the Industrial Pole of Manaus. Three executives directly involved with the strategic and financial planning processes were interviewed. The state of art mentioned in this work admits that, among the different definitions of business strategy, all schools agree concerning the necessity of generating actual and sustainable competitive advantages and defining measurable goals. The integration of strategic and financial management occurs due to the necessity of defining the sources which will allow the achievement of the established goals, also confirmed by the executives who took part in the case study. Based on this approach, this work contributes to reinforce the academic connections between strategy and finance and helps to understand the relationship between them, the importance of the financial tools and information systems. / A globalização tem contribuído para expandir os horizontes das empresas intensificando as relações entre clientes e fornecedores. A sobrevivência em um mundo cada vez mais competitivo demanda um exercício constante de fixação e acompanhamento de objetivos e metas traçadas em seu planejamento estratégico que devem ser compartilhado por toda a organização. Neste contexto, a opção pela adoção de sistemas integrados e a sofisticação do controle interno relacionado a gestão financeira e suas ferramentas a fim de proporcionar informações confiáveis preferencialmente em tempo real são vistos como diferenciais para agilizar o processo decisório. O objetivo desta dissertação é analisar aspectos da gestão estratégica e financeira, sua integração e as relações que se estabelecem entre elas. São ainda levantadas referências a ferramentas e sistemas de informação utilizados pela gestão financeira e até que ponto os dados gerados auxiliam o planejamento estratégico agregando valor. Como materiais e métodos foi realizado um estudo de caso em uma empresa de componentes do segmento eletroeletrônico do Pólo Industrial de Manaus que contou com o depoimento de três executivos diretamente envolvidos com o planejamento estratégico e financeiro da companhia. O estado da arte abordado admite que, dentre as diversas definições de estratégia empresarial, todas as escolas citadas convergem sobre a necessidade de geração de vantagens competitivas atuais e sustentáveis e a fixação de objetivos e quantificá-los. Constata-se a integração da gestão estratégica com a financeira através da necessidade de definição da origem dos recursos que irão viabilizar o alcance das metas estabelecidas para o negócio, ratificada pelas afirmações dos executivos que participam do estudo de caso. Desta forma este trabalho contribui para estreitar os laços acadêmicos entre estratégia e finanças além de auxiliar na compreensão da relação entre elas, a utilização de suas ferramentas e a crescente importância dos sistemas de informação.

Poucos estudos quantificam o risco relativo da saúde humana na transmissão spillover de doenças zoonóticas de populações de animais silvestres, estudos cruciais na compreensão da história natural das zoonoses. Coccídios, em particular os da família Sarcocystidae, são importantes agentes transmissíveis na interface homens - animais domésticos e silvestres. O diagnóstico da coccidiose é prejudicado pela limitada disponibilidade de amostras resultantes de populações animais de espécies em risco de extinção. O objetivo deste estudo foi detectar através da amplificação do locus ITS-1, protozoários das subfamílias Sarcocystinae e Toxoplasmatinae em amostras teciduais de Puma yagouaroundi, Leopardus geoffroyi, L. tigrinus, L. wiedii, L. colocolo e L. pardalis, depositados em coleções biológicas do Rio Grande do Sul, Brasil. Um objetivo adicional foi a obtenção de informações que permitissem avaliar o papel epidemiológico dos protozoários no ciclo silvestre dos parasitas e seu possível impacto sobre as populações de animais silvestres e na saúde pública. Noventa pequenos felídeos neotropicais de vida livre, representando seis espécies, foram amostrados. Destes, 31 felídeos (34,4%), de todas as seis espécies, foram positivos para Toxoplasma gondii e DNA foi detectado em 63 das 433 (14,6%) amostras de tecidos primários coletados a partir de língua (28,6%), cérebro (18,6%), músculo esquelético (17,1%), musculatura ocular (13,6%), globo ocular (13,6%), coração (11,1%), diafragma (5,4%) e humor vítreo (4,5%). Doze amostras primárias positivas ao T. gondii foram genotipadas com os marcadores moleculares SAG1, SAG2, SAG3, BTUB, GRA6, c22- 8, c29-2, L358, PK1, Apico e CS3 e a técnica multilocus PCR-RFLP, a amostra Py#36m foi totalmente caracterizada como do tipo I com alelo II no BTUB e um novo genótipo atípico Py#21M, ambos isolados de Puma yagouaroundi e nunca descrito no Brasil. Nove outras amostras tiveram caracterização parcial. Treze dos 90 felídeos foram positivos para Sarcocystis spp. (14,4%) e outros 18 felídeos, representando cinco espécies albergaram S. felis-like [Py (#75m, #83m, #35m, #20li, #55li), Lg (#80m, #70m, #88m, #71li, #67mOi), Lt (#19m, #48m, #89m, #84m), Lw (#12, #73d) e Lc (#82m, #76m)]. Um único felino de L. pardalis foi negativo. DNA do parasita foi detectado em 11,8% dos tecidos examinados (51/433): musculatura esquelética (26,5%), língua (23,2%), musculatura ocular (13,6%), diafragma (10,7%), cérebro (2,3%), coração (1,6%) e globo ocular (4,5%), nenhuma das 44 amostras de humor vítreo foi positiva. Esta é a primeira detecção e caracterização genética de T. gondii e de S. felislike em felídeos silvestres brasileiros de vida livre, demonstrando a presença destes agentes no ciclo silvestre e, a potencial transmissibilidade ao homem e a outros animais domésticos e silvestres. O uso de amostras de tecidos de animais silvestres depositados em coleções biológicas para estudos epidemiológicos de doenças monstraram serem de grande utilidade. / Few studies have quantified the relative risk of human health from spillover of zoonotic diseases from populations of wild animals; these studies are crucial for understanding the natural history of zoonoses. Coccidia, particularly from the family Sarcocystidae, are important transmissible agents at the interface of man and domestic and wild animals. The diagnosis of Coccidiosis is hampered by the limited availability of samples resulting from protection of natural populations of the species at risk of extinction. The aim of this study was to detected, by amplification of ITS-1 locus, protozoa from the subfamilies Sarcocystinae and Toxoplasmatinae in tissue samples from Puma yagouaroundi, Leopardus geoffroyi, L. tigrinus, L. wiedii, L. colocolo and L. pardalis, deposited in biological collections of the State of Rio Grande do Sul, Brazil. An additional aim was to obtain information that would enable assessment of the epidemiological role of the protozoa in the sylvatic cycle of the parasite, and its possible impact on wildlife populations and public health. Ninety free-living small wild felines, representing 6 species, were sampled. Of these, 31 felids (34.4%) of all six species were positive for T. gondii and DNA was detected in 63 of 433 (14.6%) primary tissue samples collected from the tongue (28.6%), brain (18.6%), skeletal muscle (17.1%), ocular muscles (13.6%), eye (13.6%), heart (11.1%), diaphragm (5.4%) and vitreous humor (4.5%). Twelve primary samples positive for T. gondii were genotyped with molecular markers SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and apical CS3. Using the multilocus PCR-RFLP technique, sample Py#36m was fully genotyped as Type I with allele II in locus BTUB, and a new atypical Py#21M, both isolates from Puma yagouaroundi and never described in Brazil. Nine other samples had a partial characterization. Thirteen of the 90 felids were positive for Sarcocystis spp. (14.4%) and another 18 felids, representing 5 species, harbored S. felis-like organisms [Py (#75m, #83m, #35m, #20li, #55li), Lg (#80m, #70m, #88m, #71li, #67mOi), Lt (#19m, #48m, #89m, #84m), Lw (#12, #73d) and Lc (#82m, #76m)]. A single felid of L. pardalis was negative. Parasite DNA was detected in 11.8% (51/433) of the tissues examined: muscle skeletal (26.5%), tongue (23.2%), ocular muscles (13.6%), diaphragm (10.7 %), brain (2.3%), heart (1.6%) and eye (4.5%); none of the 44 samples of vitreous humor was positive. This is the first description of the detection and genetic characterization of T. gondii and S. felis-like in free-living Brazilians wild felids, demonstrating the presence of these agents in the sylvatic cycle, and the potential transmition to humans and other domestic and wild animals. The use of tissue samples from wild animals deposited in biological collections for epidemiological studies of diseases demonstrated to be of great utility.

A novel system was developed that provides drivers lane merge advisories, using vehicle trajectories obtained through Dedicated Short Range Communication (DSRC). It was successfully tested on a freeway using three vehicles, then targeted for further testing, via simulation. The failure of contemporary simulators to effectively model large, complex urban transportation networks then motivated further research into distributed and parallel traffic simulation. An architecture for a closed-loop, parallel simulator was devised, using a new algorithm that accounts for boundary nodes, traffic signals, intersections, road lengths, traffic density, and counts of lanes; it partitions a sample, Tennessee road network more efficiently than tools like METIS, which increase interprocess communications (IPC) overhead by partitioning more transportation corridors. The simulator uses logarithmic accumulation to synchronize parallel simulations, further reducing IPC. Analyses suggest this eliminates up to one-third of IPC overhead incurred by a linear accumulation model.

The decision to select the best Intelligent Transportation System (ITS) technologies from available options has always been a challenging task. The availability of connected vehicle/automated vehicle (CV/AV) technologies in the near future is expected to add to the complexity of the ITS investment decision-making process. The goal of this research is to develop a multi-criteria decision-making analysis (MCDA) framework to support traffic agencies’ decision-making process with consideration of CV/AV technologies. The decision to select between technology alternatives is based on identified performance measures and criteria, and constraints associated with each technology. Methods inspired by the literature were developed for incident/bottleneck detection and back-of-queue (BOQ) estimation and warning based on connected vehicle (CV) technologies. The mobility benefits of incident/bottleneck detection with different technologies were assessed using microscopic simulation. The performance of technology alternatives was assessed using simulated CV and traffic detector data in a microscopic simulation environment to be used in the proposed MCDA method for the purpose of alternative selection. In addition to assessing performance measures, there are a number of constraints and risks that need to be assessed in the alternative selection process. Traditional alternative analyses based on deterministic return on investment analysis are unable to capture the risks and uncertainties associated with the investment problem. This research utilizes a combination of a stochastic return on investment and a multi-criteria decision analysis method referred to as the Analytical Hierarchy Process (AHP) to select between ITS deployment alternatives considering emerging technologies. The approach is applied to an ITS investment case study to support freeway bottleneck management. The results of this dissertation indicate that utilizing CV data for freeway segments is significantly more cost-effective than using point detectors in detecting incidents and providing travel time estimates one year after CV technology becomes mandatory for all new vehicles and for corridors with moderate to heavy traffic. However, for corridors with light, there is a probability of CV deployment not being effective in the first few years due to low measurement reliability of travel times and high latency of incident detection, associated with smaller sample sizes of the collected data.

The overall objective of this study was to test the hypothesis that insects can vector F. tumidum conidia to infect gorse plants with the aim of developing an alternative approach to mycoherbicide delivery to control weeds. Four potential insect species (Apion ulicis, Cydia ulicetana, Epiphyas postvittana and Sericothrips staphylinus) were assessed for their ability to vector F. tumidum conidia. To achieve this, the external microflora (bacteria and fungi) and the size and location of fungal spores on the cuticle of these insect species were determined. In addition, the ability of the insects to pick up and deposit F. tumidum conidia on agar was studied. Based on the results from these experiments, E. postvittana was selected for more detailed experiments to determine transmission of F. tumidum to infect potted gorse plants. The factors promoting pathogenicity of F. tumidum against gorse and the pathogen loading required to infect and kill the weed were also determined. The external microflora of the four insect species were recovered by washing and plating techniques and identified by morphology and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and sequencing of internally transcribed spacer (ITS) and 16S rDNA. A culture-independent technique (direct PCR) was also used to assess fungal diversity by direct amplification of ITS sequences from the washings of the insects. All insect species carried Alternaria, Cladosporium, Nectria, Penicillium, Phoma, Pseudozyma spp. and entomopathogens. Ninety four per cent of the 178 cloned amplicons had ITS sequences similarity to Nectria mauritiicola. E. postvittana carried the largest fungal spores (mean surface area of 125.9 ìm2) and the most fungal CFU/insect. About 70% of the fungi isolated from the insects were also present on the host plant (gorse) and the understorey grass. The mean size of fungal spores recovered from the insect species correlated strongly with their body length (R² = 85%). Methylobacterium aquaticum and Pseudomonas lutea were common on all four insect species. Pseudomonas fluorescens was the most abundant bacterial species. In the pathogenicity trials, the effectiveness of F. tumidum in reducing root and shoot biomass of 16 and 8 wk old gorse plants was significantly increased with wounding of the plants. Older plants (32 wk old) which were wounded and inoculated were significantly shorter, more infected and developed more tip dieback (80%) than plants which were not wounded (32%). This indicates that damage caused by phytophagous insect species present on gorse through feeding and oviposition may enhance infection by F. tumidum. Wounding may release nutrients (e.g. Mg and Zn) essential for conidia germination and germ tube elongation and also provide easier access for germ tube penetration. Conidial germination and germ tube length were increased by 50 and 877%, respectively when incubated in 0.2% of gorse extract solution for 24 h compared with incubation in water. Inoculum suspensions amended with 0.2% of gorse extract caused more infection and significantly reduced biomass production of 24 wk old gorse plants than suspensions without gorse extract. A minimum number of about 900 viable conidia/infection site of F. tumidum were required to infect gorse leaves. However, incorporation of amendments (which can injure the leaf cuticle) or provision of nutrients (i.e. gorse extract or glucose) in the formulation might decrease the number of conidia required for lesion formation. Scanning electron micrographs showed that germ tube penetration of gorse tissue was limited to open stomata which partly explain the large number of conidia required for infection. The flowers and leaves were more susceptible to F. tumidum infection than the spines, stems and pods. An experiment to determine the number of infection sites required to cause plant mortality showed that the entire plant needs to be inoculated in order for the pathogen to kill 10 wk old plants as F. tumidum is a non systemic pathogen. The number of infection sites correlated strongly with disease severity (R² = 99.3%). At least 50% of the plant was required to be inoculated to cause a significant reduction in shoot dry weight. F. tumidum, applied as soil inoculant using inoculated wheat grains in three separate experiments, significantly suppressed gorse seedling emergence and biomass production. In experiments to determine the loading capacity of the insect species, E. postvittana, the largest insect species studied, carried significantly more (68) and deposited significantly more (29) F. tumidum conidia than the other species. Each E. postvittana, loaded with 5,000 conidia of F. tumidum, transmitted approximately 310 conidia onto gorse plants but this did not cause any infection or affect plant growth as determined by shoot fresh weight and shoot height. E. postvittana on its own did not cause any significant damage to gorse and did not enhance F. tumidum infection. It also failed to spread the pathogen from infected plants to the healthy ones. There was no evidence of synergism between the two agents and damage caused by the combination of both E. postvittana and F. tumidum was equivalent to that caused by F. tumidum alone. This study has shown that E. postvittana has the greatest capacity to vector F. tumidum since it naturally carried the largest and the most fungal spores (429 CFU/insect). Moreover, it naturally carried Fusarium spp. such as F. lateritium, F. tricinctum and Gibberella pulicaris (anamorph Fusarium sambucinum) and was capable of carrying and depositing most F. tumidum conidia on agar. Coupled with the availability of pheromone for attracting the male insects, E. postvittana may be a suitable insect vector for delivering F. tumidum conidia on gorse using this novel biocontrol strategy. Although it is a polyphagous insect, and may visit non-target plants, F. tumidum is a very specific pathogen of gorse, broom and a few closely related plant species. Hence, using this insect species to vector F. tumidum in a biological control programme, should not pose a significant threat to plants of economic importance. However, successful control of gorse using this "lure-load-infect" concept would depend, to a large extent on the virulence of the pathogen as insects, due to the large size of F. tumidum macroconidia, can carry only a small number of it.

L'utilisation des coquilles carbonatées de foraminifères planctoniques comme marqueurs paléocéanographiques repose sur l'hypothèse fondamentale que chaque espèce morphologique correspond à une espèce biologique caractéristique d'un habitat spécifique. Cette relation empirique a été récemment remise en cause par des analyses moléculaires qui ont révélé la présence systématique de plusieurs espèces génétiques (espèces cryptiques) au sein des morpho-espèces actuelles. Il a été suggéré que ces espèces cryptiques ou génotypes, présentaient (1) des préférences biogéographiques et écologiques restreintes par rapport à leur morpho-espèce respective et (2) des différences morphologiques. Dans ce travail, nous avons caractérisé la diversité génétique, morphologique et écologique de 4 morpho-espèces clefs de la paléocéanographie, i.e. Orbulina universa, Truncorotalia truncatulinoides, Globoconella inflata et Globigerina bulloides. Cette étude repose sur le développement d'un protocole d'extraction ADN non destructif pour la coquille calcaire, permettant l'analyse conjointe de la variabilité génétique et morphologique d'un même individu. Les variations de forme ou de porosité de chacun des génotypes des morphoespèces ont été quantifiées. Il apparaît que le fort degré de plasticité morphologique largement documenté chez les foraminifères planctonique et jusqu'alors interprété comme écophénotypique, est au moins en partie la conséquence du regroupement de plusieurs génotypes présentant des morphologies et préférences écologique particulières. Sur la base de ces observations, des modèles de reconnaissance morphologique permettant d'identifier les génotypes à partir de la morphologie de la coquille, utilisables à l'échelle populationnelle, ont été développés. Afin de quantifier l'impact de l'intégration de la diversité cryptique dans les reconstitutions paléocéanographiques basées sur les assemblages de foraminifères planctoniques, les fonctions de transfert couramment utilisées ont été re-calibrées en intégrant les distributions restreintes des génotypes d'O. universa, T. truncatulinoides, G. inflata et G. bulloides. Ces re-calibrations conduisent à un degré de précision jusqu'alors jamais atteint dans les reconstructions paléocéanographiques basées sur les assemblages de foraminifères planctoniques.

La diversité fongique des meules de plusieurs espèces de Macrotermitinae a été analysée au niveau taxinomique, fonctionnel et génétique à l'aide d'une approche polyphasique associant plusieurs techniques complémentaires. L'objectif étant d'évaluer la spécificité des taxons fongiques associés aux meules ainsi que les relations qu'ils entretiennent avec les termites champignonnistes. Une grande variété de phylotypes cultivables appartenant majoritairement au phylum des Ascomycètes a été obtenue par isolement et séquençage des ITS fongiques, et peu de séquences se sont révélées être spécifiques à un genre de Macrotermitinae particulier. Les profils physiologiques obtenus ont mis en évidence la nature saprophytique de la majorité des phylotypes et confirmé l'absence de taxons spécifiques. Par PCR-DGGE de l'ADN total de meules, 100% des phylotypes ITS et 28S fongiques identifiés étaient affiliés au genre Termitomyces. La technique Suicide Polymerase Endonuclease Restriction (SuPER) a été adaptée à la mycoflore des meules pour limiter l'impact de l'ADN majoritaire du Termitomyces symbiotique. Celle-ci a permis la détection de plusieurs autres populations fongiques. Les analyses phylogénétiques ont montré d'une part la spécificité des Xylaria associés aux meules de Macrotermitinae bien qu'aucune co-évolution n'ait été observée avec les termites hôtes et d'autre part leur affiliation dans un sous-genre spécifique. Une analyse préliminaire des facteurs d'inhibition a également révélé l'implication des termites dans la régulation des communautés fongiques des meules. Dans leur ensemble, nos résultats illustrent clairement l'influence des Macrotermitinae sur les communautés fongiques telluriques pendant leurs différentes activités.