Os veículos que compõem o tráfego urbano são considerados como o principal agente causador de poluição afetando diretamente a qualidade do ar nas cidades, sendo um desafio ter mecanismos de monitoramento que possibilitem uma visão geral das condições ambientais. Para proporcionar uma visão abrangente seria necessário um grande número de estações fixas de monitoramento da qualidade do ar, além de um incremento constante para acompanhar o crescimento da cidade. Neste cenário, surge a oportunidade de instrumentação de ônibus coletivo com sensores que medem a qualidade do ar durante seu trajeto, assim aproveitando as características de mobilidade do transporte público, o qual transita em áreas povoadas, com postura dinâmica de crescimento, adaptando-se de forma coerente à mutabilidade populacional e geográfica da cidade. A presente tese apresenta novas formas de realizar análises espaçotemporais da qualidade do ar de regiões urbanas. Os modelos cartográficos criados permitem analisar regiões mapeadas usando perspectivas espaciais e temporais com informações obtidas de kits sensores móveis, para análises a cada 100 metros, promovendo uma resolução maior que os modelos tradicionais de monitoramento (escala quilométrica), os quais ainda não oferecem esta possibilidade. Esta maior resolução permite uma nova perspectiva de análises de microrregiões e, desta forma, permite que os especialista ou gestores urbanos possam entender melhor as situações, ao utilizarem as ferramentas e recursos apresentados pela presente tese. Para atingir os objetivos optou-se pela criação e validação de um kit sensor para ser hospedado em ônibus coletivo, o qual foi validado e testado, quanto à sua eficiência de medição. Este kit foi integrado a uma plataforma computacional construída para extrair e processar informações com algoritmos matemáticos. Todos os componentes da plataforma foram legitimados com experimentos que confirmaram as hipóteses inicialmente formuladas para validação. E por fim, mapeamentos com mais de 70 mil pontos foram utilizados na criação de modelos espaçotemporais para realização de estudo de caso de regiões da cidade de São Paulo, os quais demostraram os usos potenciais das proposições desta tese. / The vehicles in urban traffic are considered as the main cause of urban pollution with a direct impact on air quality, being a challenge to have monitoring tools that enable an overview of the environmental conditions influenced by vehicle emissions. One option is to instrument the entire city with low-cost fixed sensors to collect environmental data; however, to provide a comprehensive view would require a large number of sensors, and a constant increase to follow the growth of the city. In this scenario, arises the opportunity for urban bus instrumentation with sensors that measure the environmental pollution, to take advantage of the mobility characteristics of public transport, which moves in populated areas, with dynamic attitude of growth, adapting in a coherent way to population and geographic variability of the city. This PhD Thesis proposes the monitoring of air quality inside urban roads by Wireless Sensor Networks (WSN), hosted on city buses. Thus, new ways of performing spatiotemporal analyzes of the air quality of urban regions are possible with the cartographic models created by this thesis. These allow to analyze mapped regions using spatial and temporal perspectives with information obtained from mobile sensor kits, for analyzes every 100 meters, promoting a resolution higher than the traditional monitoring models (kilometer scale), which still do not offer this possibility. This higher resolution allows a new perspective of analyzes of micro regions and, in this way, allows specialists or urban managers to have a better understanding of the urban scenario. Sensor kits are created to be hosted by bus, which has been validated and tested for its efficiency in measurement. This was integrated into a computational platform built to extract and process information with mathematical algorithms. All the components of the platform were validated with experiments that confirmed the hypotheses originally formulated for validation. Finally, mappings with more than 70,000 points were used in the creation of spatiotemporal models for the study of the case of regions of the city of São Paulo, which denoted the potentials constructed by this thesis.

Le présent travail de recherche aborde la question du projet urbain appliqué dans le cas de la métropole algéroise. Dans cette ville des chantiers de grande ampleur ont été engagés: réseaux routiers et autoroutiers, grands équipements, réseaux de transport, etc. La démarche globale y a été formalisée dans le Plan Stratégique Alger 2030. Ce plan, dans les choix qu’il propose, réfère à de nombreuses expériences. Les éléments d’exécution du plan ne montrent cependant pas la prise en compte réelle de l’esprit de ces expériences. En situation d’incertitude quant à une mise en œuvre effective de projet, nous avons questionné le Plan Stratégique pour vérifier dans quelle mesure il mettait en acte les principes auxquels il réfère dans le discours. L’analyse a montré que ce plan est réduit dans les faits et les pratiques à une fonction de dessin en déficit de dessein. Ce Plan Stratégique semble éloigné de l’esprit du projet urbain : un plan qui ne s’appuie pas sur la connaissance et l’analyse du réel urbain, ni sur l’examen ou l’exploration de scénarii, ni sur ce que pourraient apporter les démarches de concertation et de débat, soit, de tout ce qui fait le projet urbain. / The present research addresses the issue of Urban Design applied to the case of Algiers metropolis. In this city, large-scale projects have been undertaken during the last decade : highway infrastructure, major equipements, urban and suburban transport networks, etc. The overall approach has been formalized in the Algiers 2030 Strategic Plan. The choices that this plan offers, are drawn from many experiences. Nevertheless, the delivery elements of the plan do not reflect the spirit of the experiences refered to. In a situation of uncertainty regarding the real execution of the project, we have examined the Strategic Plan to check how to put into practice the principles contained in its discourse. The analysis showed that the plan is reduced in practice to a plan drawing function without a purpose, an aim or a vision. This Strategic Plan seems far from the Urban Design approach : a plan that does not rely on a knowledge and an analysis of urban reality, the examination and the exploration of possible futur outcomes, nor what a consultation process and debate could bring, that is to say, all of which makes the Urban Design.

The present work aims to determine the physiological and sanitary quality of Sweet sorghum (Sorghum bicolor (L.) Moench) seeds, as well as to identify pathogens associated with seed, their transmission to seedlings and the subsequent pathogenicity of isolates obtained, In addition, molecularly identify the fungal species pathogenic to this crop. The experiments were carried out in the Teaching and Seed Research Laboratory (TSRL), of the Plant Engineering Department; In the Elocy Minussi Phytopathology Laboratory, Department of Plant Protection, and at the Biological Institute of São Paulo. Sweet sorghum seeds were used, all without chemical treatment. Sanitary quality was evaluated by sanity test, and physiological characteristics by germination and vigor tests (seedling length, dry mass, emergence, rate of emergence and accelerated aging). It was performed the test of transmission of the pathogens associated to the seeds and the subsequent pathogenicity of the obtained isolates, culminating with the molecular characterization of the identified pathogens, in which were sequenced the Internal Transcribed Spacer (ITS) genomic regions and the Elongation Factor 1 - alpha (TEF1-α) gene. The design used was the completely randomized design, with four cultivars of Sweet sorghum (BRS 506, F19, BRS 511 and BRS 509); For the evaluation of pathogenicity, the factorial scheme is represented by four cultivars and three isolates of Fusarium spp., besides the witness. The seeds of the BRS 509 cultivar were considered to have lower physiological quality than the other cultivars. The DNA sequencing allowed identifying the Fusarium thapsinum species as a pathogenic agent in the sweet sorghum crop, and proven its transmission via seeds. / O presente trabalho teve como objetivo determinar a qualidade fisiológica e sanitária de sementes de cultivares de sorgo sacarino (Sorghum bicolor (L.) Moench), bem como identificar os patógenos associados à semente, sua transmissão às plântulas e a posterior patogenicidade de isolados obtidos, além disso, identificar molecularmente as espécies fúngicas patogênicas a esta cultura. Os experimentos foram realizados no Laboratório Didático e de Pesquisas em Sementes (LDPS), do Departamento de Fitotecnia; no Laboratório de Fitopatologia Elocy Minussi, do Departamento de Defesa Fitossanitária e, no Instituto Biológico de São Paulo. Foram utilizadas sementes de sorgo sacarino, todas sem tratamento químico. A qualidade sanitária foi avaliada pelo teste de sanidade, e as características fisiológicas por meio dos testes de germinação e de vigor (comprimento de plântulas, massa seca, emergência, índice de velocidade de emergência e envelhecimento acelerado). Foi realizado o teste de transmissão dos patógenos associados à semente e a posterior patogenicidade dos isolados fúngicos obtidos, culminando com a caracterização molecular dos patógenos identificados, na qual foram sequenciadas as regiões genômicas Internal Transcribed Spacer (ITS) e o gene do fator de elongação 1-α (TEF1α). O delineamento utilizado foi o inteiramente casualizado com quatro cultivares de sorgo sacarino (BRS 506, Fepagro 19, BRS 511 e BRS 509); já para a avaliação da patogenicidade, o esquema fatorial foi representado pelas quatro cultivares e três isolados de Fusarium sp., além da testemunha. As sementes da cultivar BRS 509 foram consideradas de qualidade fisiológica inferior as demais cultivares. O sequenciamento de DNA permitiu identificar a espécie Fusarium thapsinum como agente patogênico na cultura do sorgo sacarino, sendo comprovada sua transmissão via sementes.

This diploma thesis examines the impact of the amendment to the Act on Budgeting of Taxes, effective from 1 January 2013, on the financing of municipalities setting up a school in their territory. The main aim of the diploma thesis is to find out what influence the amendment to the School Act and the Act on the Budgeting of Taxes has on the financing of municipalities establishing school and municipalities that do not establish school in their territory. Furthermore, whether the municipality, which establishes a school in its territory, is able to cover the costs incurred by education when fees are abolished, and the pupils from the municipalities in whose territory the school is not established are also able to attend. The diploma thesis deals with the issue of financing the municipality, which establishes a school and a municipality that does not establish a school in its territory. Then the issue is related to the whole Pardubice region, where I find out whether the municipalities setting up the school in their territory will be able to cover the costs related to the operation of the school after the amendment of the Act on Budgeting of Taxes.

Challenges arising from an increasing traffic demand, limited resource availability and growing quality expectations of the customers can only be met successfully, if each transport mode is regarded as an intelligent transportation system itself, but also as part of one intelligent transportation system with “intelligent” intramodal and intermodal interfaces. This topic is well reflected in the Third International Conference on “Models and Technologies for Intelligent Transportation Systems” which took place in Dresden 2013 (previous editions: Rome 2009, Leuven 2011). With its variety of traffic management problems that can be solved using similar methods and technologies, but with application specific models, objective functions and constraints the conference stands for an intensive exchange between theory and practice and the presentation of case studies for all transport modes and gives a discussion forum for control engineers, computer scientists, mathematicians and other researchers and practitioners. The present book comprises fifty short papers accepted for presentation at the Third Edition of the conference. All submissions have undergone intensive reviews by the organisers of the special sessions, the members of the scientific and technical advisory committees and further external experts in the field. Like the conference itself the proceedings are structured in twelve streams: the more model-oriented streams of Road-Bound Public Transport Management, Modelling and Control of Urban Traffic Flow, Railway Traffic Management in four different sessions, Air Traffic Management, Water Traffic and Traffic and Transit Assignment, as well as the technology-oriented streams of Floating Car Data, Localisation Technologies for Intelligent Transportation Systems and Image Processing in Transportation. With this broad range of topics this book will be of interest to a number of groups: ITS experts in research and industry, students of transport and control engineering, operations research and computer science. The case studies will also be of interest for transport operators and members of traffic administration.

El objetivo del presente estudio fue describir las percepciones de los usuarios respecto a la consejería brindada en los Centros Referenciales para la atención de las Infecciones de Transmisión Sexual (CERITS), aplicando un diseño combinado de tipo cuanti- cualitativo. La población de estudio estuvo conformada por 144 usuarios: 72 Hombres que hacen Sexo con Hombres (HSH) y 72 Trabajadores Sexuales (TS) y consejeros de seis CERITS de Lima y Callao. Para la recolección de datos se combinó: una encuesta estructurada, grupos focales y clientes simulados con usuarios, un flujograma de procesos y entrevistas de profundidad a seis consejeros. La mayoría de los usuarios (90%) perciben como buena la consejería, sin embargo cualitativamente se han identificado disconformidad relacionados a la falta de privacidad y comodidad de los ambientes, a la irregularidad en la provisión de medicamentos e insumos y a comportamientos discriminatorios del personal de salud. Por el contrario las consejeras percibieron la atención en la consejería como deficiente porque lo asocian a los problemas de gestión. / A quantitative, qualitative and tri-phasic study was performed in order to describe the perceptions of users regarding the quality of counseling provided at Reference Centers concerning Sexually Transmitted Infections (RCSTI). The study population consisted of 144 users: 72 Men who have sex with men (MSM) and 72 sex workers (SW) and six RCSTI of metropolitan Lima. The tools for collection’s data were: a structured survey, focus groups and customer simulated situations, process flowchart design and in-depth interviews to six counselors. Most users perceive counseling as something good, however qualitatively some perceptions of disagreement were identified mainly related to the lack of privacy environments, the irregularity in the provision of medicines and supplements and discriminatory behavior of health personnel. It is noteworthy that the counselors perceive the service provided to users as deficient because they associate it to management problems which limit them to meet the standards set by current regulations. Keys Words:  Referential Centers for the care of Sexually Transmitted Infections and HIV AIDS (RCSTI).  Periodic Health Care (PHC).  STI HIV AIDS Counseling.  Vulnerable Population: Man doing Sex with Man (MSM) and Sex Workers (WS).

Intelligent Transport Systems (ITS) are aiming to provide innovative services related to different modes of transport and traffic management, and enable various users to be better informed and make safer, more coordinated and smarter use of transport networks. Cooperative-ITS (C-ITS) support connectivity between vehicles, vehicles and roadside infrastructure, traffic signals as well as with other road users. In order to enable vehicular communications European Telecommunication Standards Institute (ETSI) delivered ITS-G5 -- a of set of C-ITS standards. Considering the goals of C-ITS, inter-vehicle communications should be reliable and efficient. In this thesis we study the performance, efficiency, and dependability of ITS-G5 communications for Cooperative adaptive cruise control (C-ACC) and platooning C-ITS applications. We provide an overview of currently available and ongoing standardization targeting communications in C-ACC/platooning. We study the performance of ITS-G5 beaconing in a C-ACC/platooning scenario, where we show that its performance may deteriorate when implemented in cooperative driving applications due to the kinematic-dependent design of the message triggering mechanism. We explain in detail the cause of this phenomenon and test it for a wide range of parameters. Also, we study the influence of different available ITS-G5 legitimate setups on the C-ACC/platooning fuel efficiency and demonstrate that proper communication setup may enhance fuel savings. This thesis also proposes a jamming denial-of-service attack detection algorithm for platooning. The main advantage of our detector is its short learning phase that not exceed a second and low detection delay of a few hundreds of milliseconds. Under some assumptions, the proposed algorithm demonstrates the ability to detect certain types of attacks with average probability above 0.9. / ACDC

Eurybia et ses proches parents Oreostemma, Herrickia et Triniteurybia sont appelés le grade des eurybioïdes. Comprenant 31 espèces vivaces, ce grade appartient au clade Nord-américain de la tribu des Astereae. Les analyses moléculaires antérieures ont montré que ce groupe est à la fois paraphylétique aux Machaerantherinae et un groupe frère aux Symphyotrichinae. Les relations infragénériques partiellement résolues et faiblement supportées empêchent d’approfondir l'histoire évolutive des groupes et ce, particulièrement dans le genre principal Eurybia. Le but de cette étude est de reconstruire les relations phylogénétiques au sein des eurybioïdes autant par l'inclusion de toutes les espèces du grade que par l’utilisation de différents types de régions et de méthodes d'inférence phylogénétique. Cette étude présente des phylogénies basées sur l'ADN ribosomal nucléaire (ITS, ETS), de l'ADN chloroplastique (trnL-F, trnS-G, trnC-ycf6) et d’un locus du génome nucléaire à faible nombre de copie (CNGC4). Les données sont analysées séparément et combinées à l’aide des approches de parcimonie, bayesienne et de maximum de vraisemblance. Les données ADNnr n’ont pas permis de résoudre les relations entre les espèces polyploïdes des Eurybia. Les analyses combinées avec des loci d’ADNnr et d’ADNnr+cp ont donc été limitées à des diploïdes. Les analyses combinées ont montré une meilleure résolution et un meilleur support que les analyses séparées. La topologie de l’ADNnr+cp était la mieux résolue et supportée. La relation phylogénétique de genres appartenant au grade des eurybioïdes est comme suit : Oreostemma (Herrickia s.str. (Herrickia kingii (Eurybia (Triniteurybia - Machaerantherinae)))). Basé sur la topologie combinée de l’ADNnr+cp, nous avons effectué des analyses de biogéographie à l’aide des logiciels DIVA et LaGrange. Ces analyses ont révélé une première radiation des eurybioïdes dans l’Ouest de l’Amérique du Nord, suivi de deux migrations indépendantes dans l’Est de l’Amérique du Nord chez les Eurybia. Due au relatif manque de variabilité de l’ADNnr, l’ADNcp et CNGC4, où le triage de lignés incomplet était dominant, l'origine du grade est interprétée comme récente, possiblement du Pliocène. La diversification du groupe a été probablement favorisée par les glaciations Pléistocènes. / Eurybia and it relatives, Oreostemma, Herrickia, and Triniteurybia, are collectively called the eurybioid grade. Comprising 31 perennial species, this grade belongs to the North American clade of the tribe Astereae. Early molecular analyses had inferred that this group is paraphyletic to the Machaerantherinae and sister to the Symphyotrichinae. The partially resolved and poorly supported relationships at the infrageneric level within the group, particularly within the core genus Eurybia, is preventing further insights into the evolutionary history of the group. The aim of this study is to reconstruct the phylogenetic relationships among the eurybioids by including all species of the grade and by using both different types of regions and multiple phylogenetic inference methods. The present study provides phylogenies based on nuclear ribosomal DNA (ITS, ETS), chloroplastic DNA (trnL-F, trnS-G, trnC-ycf6), and a low-copy nuclear locus (CNGC4), in separate and combined datasets analyzed using maximum parsimony, Bayesian and maximum likelihood approaches. In a separate analysis of the nrDNA dataset, the relationships of polyploids in Eurybia proved to be impossible to resolve. The nrDNA and nr+cpDNA combined analyses therefore were restricted to diploids. The combined analyses provided greater resolution and support than separate analyses. The nr+cpDNA phylogeny was the best resolved and supported. The phylogenetic relationship of genera belonging to the eurybioid grade is as follows: Oreostemma (Herrickia s.str. (Herrickia kingii (Eurybia (Triniteurybia – Machaerantherinae)))). Based on the nr+ cpDNA combined topology, we performed biogeographical analyses using DIVA and LaGrange. These analyses revealed an initial radiation of the eurybioids in western North America, with two independent migrations to eastern North America within Eurybia. Based on the relative lack of variation in nrDNA, cpDNA and CNGC4, where incomplete lineage sorting was dominant, the origin of the grade is interpreted as recent, probably from the Pliocene. Diversification of the group was probably favored by the Pleistocene glaciations.

La culture de saules (Salix sp.) est une pratique courante en Europe et en Amérique du Nord pour produire de la biomasse végétale. Cependant, le développement d’outils moléculaires est très récent. De plus, la phylogénie des saules est incomplète. Il y a un manque d’information pour les programmes de sélection d'espèces indigènes et pour la compréhension de l’évolution du genre. Le genre Salix inclut 500 espèces réparties principalement dans les régions tempérées et boréo-arctique de l’hémisphère nord. Nous avons obtenu l’ensemble des espèces retrouvées naturellement en Amérique (121 indigènes et introduites). Dans un premier temps, nous avons développé de nouveaux outils moléculaires et méthodes : extraction d’ADN, marqueurs microsatellites et gènes nucléaires. Puis, nous avons séquencé deux gènes chloroplastiques (matK et rbcL) et la région ITS. Les analyses phylogénétiques ont été réalisées selon trois approches : parcimonie, maximum de vraisemblance et Bayésienne. L’arbre d’espèces obtenu a un fort support et divise le genre Salix en deux sous-genres, Salix et Vetrix. Seize espèces ont une position ambiguë. La diversité génétique du sous-genre Vetrix est plus faible. Une phylogénie moléculaire complète a été établie pour les espèces américaines. D’autres analyses et marqueurs sont nécessaires pour déterminer les relations phylogénétiques entre certaines espèces. Nous affirmons que le genre Salix est divisé en deux clades. / Fast growing willows (Salix sp.) are increasingly used in Europe and North America for biomass production and other environmental applications. However, the development of molecular tools is recent. The phylogeny of willows is incomplete, which slows down the selection of suitable native species and the development of improvement programs. The genus Salix includes approximately 500 species worldwide, and these are mainly located in temperate and cold regions of the Northern Hemisphere. We gathered leaf material from all 121 willows of North America (species native and introduced). We developed three molecular tools-methods: DNA extraction, SSR markers, and nuclear genes. We sequenced two chloroplast genes matK and rbcL and the ITS region. Phylogenetic analyses were carried out using parsimony, maximum likelihood and Bayesian approaches. The species tree provides strong support for a division of the genus into two subgenera, Salix and Vetrix. Sixteen species have ambiguous positions. A complete molecular phylogeny of American willows has been established. It needs to be confirmed and further resolved using other molecular data. Nonetheless, the genus clearly has two clades.

This thesis focuses on the historical figure of Leopoldina of Austria, daughter of the Austrian Emperor Francis I. Married in 1817 with the Portuguese Prince Pedro, the future emperor of Brazil. Chapter one. Introduces the reader in the context of the Portugal of the beginning of the nineteenth century including the departure of the royal court to Brazil and events occured during the period 1808 - 1822 in Brazil that led to the declaration of independence. Chapter two. Devoted to the Archduchess Leopoldina and her biography, with an important remark to the links established between Central Europe and Brazil, thanks to the marriage of Leopoldina and Pedro. Chapter three. Refers to Leopoldina's contribution to the independence of Brazil, which is neglected by some historians. The chapter describes the two main currents developed by historians: That one that attributes the independence achievement mainly to Pedro I, neglecting the influence of the Empress Consort on the process, revised through a selection of handbooks of Brazilian history; and the one, supported in the writings of Karl H. Oberackera Jr., assigning to Leopoldina a fundamental role during the declaration of independence of Brazil. Key words: Leopoldina of Austria, declaration of independence, Pedro I