Professional learning communities (PLCs) are being recognized as a leading strategy to improve student achievement. Trust is critical in effectively implementing the PLC model, and the school principal is best positioned to influence school trust levels. Using Hoy and Tschannen-Moran's (1999) five facets of trust, this research sought to clarify the impact of trust among PLC teachers on their team's collaborative practices. Focus group data were collected from 12 collaborative teams in 4 schools. Six of the teams were from one school that was struggling to implement the PLC model; the other six teams were from three schools that were implementing the model successfully. This research utilized a matched cases case study to understand the relationship between trust and collaboration in PLC teams. Findings suggested the teams in successful PLCs built trust through treating one another with patience and kindness, fulfilling personal responsibilities, and sharing personal information. Additionally, the principal influenced team members' trust by allowing autonomy and team formation input. Perceived benevolence and competence led to teachers sharing teaching strategies, being more open with student data, and teaching one another's students. Also successful and non-successful PLCs emphasized different facets of trust in describing development of trust, the principal's role in building trust, and the role of trust in collaboration. These findings can inform school leaders how to more effectively build and preserve trust among members of collaborative teams such as PLCs.

This study was conducted for the primary purpose of discovering the relationship of two personal variables to effective seminary teaching.It was hypothesized that:(1) There is no significant difference between seminary teachers whom the department considers as very effective (high-rated) and seminary teachers whose teaching effectiveness is questioned by the department (low-rated) in their ability to empathize with the self concept of their students.(2) there is no significant difference between seminary teachers who are high-rated and seminary teachers who are low-rated in their ability to sociempathise (perceive student's social status among peers).

The purpose of this case study was to investigate the relationship between the implementation of the District Professional Learning Community (DPLC) model of professional development and collective teacher efficacy and organizational trust at one middle school in a large urban school district. Data were collected from the following sources: Goddard & Hoy's (2003) CE Scale Form L, Hoy & Tschannen-Moran's (2003) Omnibus-T scale, six additional survey items used to explore teachers' perceptions of the DPLC model's influence on improving student literacy, and semi-structured focus group interviews. A series of analysis of variance (ANOVA) and chi-square tests were performed to analyze the survey data. Focus group interview data were examined using a priori codes, open codes, in vivo codes, and logic model analytics. The findings of this study revealed that the DPLC model has a positive impact on collective teacher efficacy and organizational trust at Central Florida Middle School. Additional statistically significant findings include: (a) increase in faculty trust in principal over time; (b) increase in faculty trust in colleagues over time; (c) greater increase in collegial trust among English Language Arts/Reading teachers as compared to other content area peers; (d) members of the DPLC Site Team report greater knowledge and utilization of learned literacy strategies as compared to non-members. Through this investigation of teacher perceptions, truths about organizational culture were revealed. The results of this study confirm and expand the research supporting the positive impact of distributed leadership practices and effective professional development on collective teacher efficacy and organizational trust.

Sphingosine 1-phosphate (S1P) is a lipid chemoattractant molecule. Once formed, S1P can be transported extracellularly by S1P transporters spinster homolog 2 (Spns2) or major facilitator domain containing 2B (mfsd2b). In the extracellular space, S1P can bind to S1P-specific G-protein coupled receptors (S1PR), which initiate many signaling pathways. A critical role of extracellular S1P is its ability to cause lymphocyte egress, which can have implications for inflammatory and autoimmune diseases. For this reason, there has been a growing interest in exploring potential spns2 inhibitors to further elucidate their therapeutic potential. Initial screenings confirmed that fused heterocycles, including phthalimide and benzoxazoles, demonstrated moderate inhibition of Spns2 using a HeLa cell assay. An extensive structure-activity relationship (SAR) study of these scaffolds was performed to analyze the impact of various amine head groups, regioisomers, and alkyl tails on performance. It was determined that 2-aminobenzoxazoles with secondary amines were potent inhibitors of the transporter. Additionally, the position of the lipophilic tail moiety played a large role in activity. From these modifications, SLB1122168 (2.44p) was found to be our lead compound. It was determined that (2.44p) had an IC50 of 94 ± 6 nM and was shown to be efficacious in decreasing lymphocyte count by 55% in a dose-dependent manner in both rat and mice models. The discovery of (2.44p) can serve as a novel chemical tool to investigate Spns2 biology and use it as a probe to determine the potential of Spns2 as a drug target. Organoboron compounds are useful synthetic intermediates in forming C-X, C-C, and C-H bonds. One way to synthesize these compounds is through copper catalysis. Copper is favorable to other transition metals because it is an Earth-abundant, low-cost metal that can be utilized in regio- and stereoselective reactions. Conjugated 1,3-enynes are important functional groups that iii are found in active natural products, organic synthetic intermediates, and materials. Previous methods used rare transition metals, designer ligands, or harsh acidic conditions to synthesize such compounds. In this dissertation, we developed a stereoselective one-pot copper-catalyzed semi-reduction of 1,3-diynes to produce (Z)-1,3-enynes. This method uses Cu(OAc)2, HBpin and Xantphos to successfully synthesize (Z)-1,3-enynes that were tolerated well over a broad substrate scope, including heterocyclic, alkyl, and aryl substituents. It was determined that this reaction went through a 2-boryl intermediate which was facilitated by a CuH species. / Doctor of Philosophy / Autoimmune diseases are caused by immune cells attacking healthy cells. The signaling lipid sphingosine-1-phosphate (S1P) plays a major role in trafficking immune cells, in which immune cells follow the S1P gradient from low concentrations (secondary lymphoid tissues) to high concentrations (lymph). In the case of multiple sclerosis, immune cells can attack healthy neurons that cause a myriad of symptoms. Currently, there are four drugs approved by the Food and Drug Administration (FDA) targeting the S1P pathway for multiple sclerosis. In all cases, these drugs act as S1P-receptor (S1PR) functional antagonists, which decreases the amount of extracellular S1P, which in turn decreases the immune cells in the lymph that can attack healthy cells. Unfortunately, all four drugs exhibit on-target cardiovascular side effects. To circumvent the on-target side effects seen in current FDA-approved drugs, other nodes of the S1P pathway have been assessed for multiple sclerosis. One node of interest is spinster homolog 2 (Spns2), a transporter of S1P, whose inhibition has also been shown to decrease extracellular S1P. In this dissertation, we will be assessing various inhibitors for their in vitro and in vivo properties. 1,3-Enynes are a functional group found in medicinally relevant compounds and can be used as intermediates to make more complex compounds. Current methods to make this functional group use expensive rare metals or harsh acidic conditions. We developed new methods that utilized copper, an abundant metal, and boron, an atom whose empty p orbital allows for unique reactivity. Utilizing a copper-hydride species allowed us to semi-reduce 1,3-diynes to (Z)-1,3-enynes, where water was used instead of acid to allow for the semi-reduction to occur. This reaction was shown to tolerate a wide range of substrates and gave good to excellent yield.

One of the most difficult tasks a compiler writer faces is the construction of the instruction selector. The instruction selector is that part of the compiler that translates compiler intermediate representation (IR) into instructions for a target machine. Unfortunately, implementing an instruction selector “by hand” is a difficult, time consuming, and error prone task. The details of both the IR and target instruction set must be carefully considered in order to generate correct and efficient code. This, in turn, requires an expert in both the compiler internals as well as the target machine. Even an expert, however, can implement an instruction selector that is difficult to verify and debug. In this dissertation we describe the instruction selector problem, cover previous attempts at solving it, and identify what we believe to be the most prominent factor inhibiting their widespread adoption. This dissertation proposes a generalized approach toward generating instruction selectors automatically. In particular, we propose CISL, a common machine description language for specifying the semantics of compiler IR and target instructions, and GIST, a machine independent heuristic search procedure that can find equivalent instruction sequences between compiler IR and target instructions. CISL is an object-oriented-based language leveraging modern programming language constructs (e.g., classes, inheritance, mixins) and is capable of describing instructions for a variety of IR and target ISAs (Instruction Set Architecture). GIST leverages CISLs well-defined semantics and a canonicalization process to discover automatically instruction selector patterns: target instruction sequences that implement IR semantics. These instruction selector patterns are then generated in a compiler implementation independent format (XML). Small adapter programs use the generated instruction selector patterns to generate compiler specific implementation code. Our experiments show that instruction selector patterns can be discovered automatically and independent of a particular compiler framework or target machine. In addition, experience proved that adapter programs are easy to implement and instruction selector code is easy to generate from generated patterns. Furthermore, the generated instruction selectors are comparable in performance to the original compilers.

<p>  </p> <p>The development of a novel technique employing the use of a linear quadrupole ion trap mass spectrometer coupled to a Nd:YAG laser and a home-built fast reagent-mixing apparatus is detailed and used to detect the short-lived tetrahedral reaction intermediate of the reaction of acetyl chloride with ethanol in microdroplets. Additionally, tandem and high-resolution mass spectrometry is used to characterize potential precursors for solution-processed metal selenide semiconductors in order to determine a synthetic route to sulfur-free thin films. Lastly, Raman MCR (multivariate curve resolution) spectroscopy is used to study the binding-induced conformational change of 1-bromopropane upon binding to α-cyclodextrin as a model system to examine guest conformational changes upon binding to a host molecule.</p>

This work analyzes the design and implementation of waveguides used to measure stress waves in solid mechanics via explicit finite element analysis and experimentation. Many areas of physics use waveguides where control of timing, location, or frequency of waves is imperative to functionality of a system. Split Hopkinson pressure bars (Kolsky bars) traditionally utilize straight waveguides during testing. Prior research produced the first bent wave guide for use in such an application, the coaxially embedded serpentine bar (CESB). Explicit finite element analysis (FEA) provides a modeling approach to understand the effects of pass and joint geometry and boundary conditions on the functionality of solid-mechanic waveguides like the CESB. FEA and experimentation also contrasts the functionality of welded joints and threaded joints. Novel waveguide designs that do not feature tubes are also detailed for use in dynamic mechanical testing and dynamic hardness indentation experiments. These designs feature acoustic lengths up to two orders of magnitude greater than their physical lengths.

Stone plays an inextricable role in the lives of Andean peoples and the monumental stoneworks of pre-Hispanic cultures stand in memorial to the experiences and beliefs of those who created them. Stone is often selected as a medium for symbolic works due to its durability and perceived permanence, but in the Andes, its meaning expands beyond its physical properties. Stone was an extension of the animate landscape that both sheltered and endangered its inhabitants. Stories were attached to stones, whether natural or modified, to embed knowledge of the landscape and of history in the memory of communities. Centuries later, archaeologists utilize modified stones and constructed monuments as a window to understand long past societies. As our own technological abilities expand, we are able to garner even deeper understandings of the way stones were used and the meanings they may have once held. High in the Peruvian Andes, in a small city renown for its natural beauty and ecological adventures, there is a modest museum, where hundreds of once powerful stone ancestors are visited by school groups and tourists, receiving words of wonder in place of the offerings of coca, chicha, and music once granted to them by their human children and grandchildren known today as the Recuay people. These carved figures give clues to their meaning through their crouched mummified positions and their accoutrements of power, warfare, and fertility. But much of their histories have been lost, as looting, religious persecution, and local curation have moved almost all of these ancestors from their resting places, erasing clues about their roles and meaning in the society that made them. Utilizing a Holistic Approach to craft production (Shimada and Craig 2013; Shimada and Merkel 1987; Shimada and Wagner 2007), this research seeks to recontextualize these powerful Recuay ancestors that once populated the Huaraz region of highland Ancash (ca. 100-700 CE) through an investigation of their making. Each choice and action in the process of production reveals important information about broader technological systems, social, political, and economic relationships, and the cosmologies and belief systems of the makers. Incorporating multiple lines of evidence from geochemical and technological analysis, as well and surveys of archaeological sites, interviews with modern stone sculptors, and experimental testing of manufacturing techniques, this research provides a reconstruction of the entire production sequence for Recuay stone ancestors, from the selection, procurement, and dispersal of raw materials to the techniques, tools, and settings employed in manufacturing. This research offers an example of the efficacy of the Holistic Approach to gain sociocultural insights from material records of the process of production through direct evidence of manufacturing and to overcome limitations regarding artifact provenience. Additionally, the robust geochemical analysis outlined here provides a replicable approach to semi-quantitative sourcing studies through non-destructive portable X-Ray Fluorescence spectroscopy, with an analytical approach that is as accessible as equipment operation. As a rare case study in pre-Inkaic stone quarrying and carving, this research showcases the technological and symbolic variability within a centuries long belief system that recognized the animate landscape and treated extracted materials as an extension of those forces. Over the course of this 600 year long carving tradition, Recuay artisans altered the forms and iconographic details of these important sculptures, but the production techniques, surface treatments, and raw materials remained remarkably consistent. Only four geologic sources provided raw materials for 96% of analyzed sculptures in this regional assemblage across three different volcanic stone types, including two long-hypothesized quarries, Pongor and Cerro Walun. Over 97% of sculptures across all volcanic, sedimentary, and plutonic stone types shared a specially crafted surface treatment that differed from other Recuay stoneworks and from stone sculptures of preceding cultures in the region. Investigations at the confirmed quarry site of Cerro Walun reveal contextualized insights about the infrastructure of stone quarrying and carving and its close association with tombs and venerated, animate landscapes. Combined with understandings of communal ancestor veneration and intercommunity socio-political negotiations among the Recuay, we see that these stone figures and the process of creating them played an active role in the expression and maintenance of relationships and knowledge between communities and across generations.

Lack of hair due to alopecia or skin grafting procedures causes significant distress due to hair's role in social and sexual communication. Only limited pharmacological agents are currently available to stimulate hair growth; their development is hampered by inappropriate model systems. Most research involves large terminal scalp follicles rather than the clinical targets of tiny vellus or intermediate follicles. The overall aim of this thesis was to develop a novel model system based on intermediate hair follicles. Initially, intermediate follicles from female pre-auricular skin were characterised and compared to matched terminal follicles. Intermediate follicles were smaller, less pigmented, shorter and possessed a more 'tubular' bulb morphology than their more 'bulbous' terminal counterparts. Significant correlations were demonstrated between various hair follicle measurements and corresponding dermal papilla diameters. Isolated terminal follicles grew significantly more than intermediate hair follicles in organ culture for 9 days. Testosterone (10nM), the major regulator of human hair growth, increased only intermediate follicle growth; the anti-androgen, cyproterone acetate (1¿M), prevented this stimulation, unlike the 5¿-reductase type 2 inhibitor finasteride (40ng/ml). Immunohistochemistry demonstrated androgen receptor and 5¿-reductase type 2 proteins in both follicle types, while quantitative real-time PCR and gene microarray analysis detected their increased gene expression in intermediate follicles. Thus, smaller intermediate follicles showed major morphological and gene expression differences to terminal follicles in vivo and retained significant, biologically-relevant differences in vitro in organ culture including androgen-responsiveness. Therefore, intermediate hair follicles offer a novel, exciting, more clinically relevant, albeit technically difficult, model for future investigations into hair growth.

The spatial and population dynamics of avian scavengers are poorly understood. This information is key for management and conservation interventions that guarantee long-term species conservation. My goal in this dissertation is to fill information gaps on the movement ecology of New World vultures using the Turkey Vulture (Cathartes aura) as a model species. I used a continental-wide satellite telemetry dataset to study the migration ecology, space-use, and demography of the three North American breeding populations of Turkey Vultures wintering in the Neotropics during a 17-year period. I found that primary productivity, but not weather, triggered Turkey Vulture migratory behavior, migration initiated when primary productivity dropped at the end of the breeding and non-breeding season. Migratory connectivity was high at the species level (0.85, 95% CI: 0.74–0.94). However, I found evidence of intrapopulation segregation during the non-breeding season demonstrated by lower values of migratory connectivity in each population. I investigated how seasonality interacted with human disturbance, landscape composition and configuration to mediate patterns of geographic and environmental space-use, and annual and seasonal survival probabilities. Environmental space-use was best explained by landscape configuration. Geographic space-use exhibited a quadratic response to landscape configuration metrics, suggesting that Turkey Vultures maximize space-use in landscape with intermediate disturbance. Human disturbance, but not but not landscape composition and configuration, influenced survival rates in space and time. Overall annual survival averaged 0.87 (95% CI = 0.74 – 0.98). Mortality risk was low in western and central populations but was 3.7 times greater for vultures in the eastern population. Risk of mortality for all vulture populations increased with road density, and this was greater during the non-breeding and return migration seasons. My results suggest that spatial and population dynamics are affected at a continental scale by the energy landscape, intermediate disturbance and human disturbance. My dissertation emphasizes the importance of an integrative empirical-modeling approach to address questions on effects of resources availability and search efficiency in the spatial and population dynamics of avian scavengers.