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E-cadherin (Cdh1), is a major component of epithelial adherens junctions, binds the Wnt pathway effector β-catenin and is lost at the invasive edge of colon cancers. Crypt stem cells give rise to 4 cell lineages that, with the exception of Paneth cells, travel along the crypt-villus axis over 3 days and are shed by anoikis. Gain of function of the Wnt pathway by a mutation in Adenomatous Polyposis Coli (Apc) disrupts enterocyte turnover to result in adenoma formation. Homozygote null Cdh1 is embryonic lethal, and heterozygote Cdh1 can promote Apc^1638N induced adenoma formation, but we lack models that assess additional functions of Cdh1 in the adenoma to carcinoma transition. The aim of this thesis was to evaluate the effect of Cdh1 loss of function in the murine intestine, including in the setting of Wnt pathway activation. To do this, germline and intestinal specific conditional Cdh1 loss of function models were generated. Conditional homozygous deletion of Cdh1 resulted in embryonic lethality using Villin-Cre. In adults, homozygous Cdh1 loss using the tamoxifen inducible Villin-CreER^T2 led to intestinal inflammation, bacteraemia and disrupted crypt-villus architecture. Combined conditional homozygous deletion of Cdh1 and Apc resulted in Wnt pathway upregulation assessed by β-catenin immunolabelling. Strain dependent effects of Cdh1 heterozygosity were apparent on the Apc heterozygote background: Apc^Min/+ Cdh1^+/- (C57BL/6J) had no effect on survival or adenoma phenotype compared to littermate Apc^Min/+; Cdh1^+/fl increased adenoma burden in Apc^+/fl Vil-Cre animals (B6D2/C57BL/6J). Low frequency recombination of Apc^fl/fl using Lgr5-EGFP-IRES-CreER^T2 bypasses the loss of heterozygosity event relied on in heterozygous Apc tumour models achieving a large adenoma burden within 4 weeks. Cdh1 loss did not alter survival or adenoma phenotype in this model, including the development of large caecal tumours. Immunolabelling of tumours from Apc^fl/fl Cdh1^fl/fl animals showed persistent E-cadherin protein expression, suggesting incomplete recombination or that double homozygote enterocytes failed to survive. In vitro adenoma culture was used to test whether E-cadherin loss was incompatible with enterocyte survival in the setting of Wnt activation. Apc^fl/fl Cdh1^{+/+} and Apc^fl/fl Cdh1^fl/fl adenoma were cultured in matrigel and treated with an adenovirus expressing Cre recombinase under a CMV promoter (Ad-Cre). Ad-Cre had no effect on Apc^fl/fl Cdh1^+/+ adenoma growth. Ad-Cre treated Apc^fl/fl Cdh1^fl/fl adenoma organoids showed cells where E‑cadherin loss resulted in Wnt pathway upregulation as assessed by nuclear β-catenin and Axin2 expression, and epithelial mesenchymal transition shown by upregulation of fibronectin, twist and vimentin. This work supports a role for E-cadherin in modulation of the Wnt pathway. Further investigation is required to define the cell adhesion versus Wnt regulatory functions of E-cadherin.

The nuclear factor kappa B (NFKB) signalling pathway is essential in the establishment and propagation of inflammation in the intestine. An increased number of cells, predominantly of the macrophage and intestinal epithelial cell (IEC) type, are known to contain the active form of the NF1d3-p65 subunit in inflamed and noninflamed intestinal tissue from Crahn's disease (CD) patients, though this remains to be confirmed. However the stimuli that induce NFKB activation in IECs and the mechanism of NFKB activation in macrophages, are only poorly understood. As such, this thesis has investigated the NFKB signalling pathway and its role in intestinal inflammation. Increased levels of NFKB DNA-binding activity and inhibitor kappa B alpha (IKBa) protein levels were found in both inflamed and non-inflamed intestinal tissue from CD patients. However, Bcl-3 levels did not significantly change. In HeLa Ohio cells, a human mucosal epithelial cell line, interleukin-l ~ (IL-l ~), lipopolysaccharide (LPS) and Phorbol 12-myritate I3-acetate (PMA) were shown to induce NFKB activation. However, when these same stimuli were used in another human IEC line, Caco-2, little NFKB-mediated gene expression was observed unless a combination of stimuli, IL-l~, LPS and tumour necrosis factor alpha (TNFa), was used. In RAW 264.7 cells, a murine macrophage cell line, LPS-stimulated NFKBmediated NO production was shown to involve protein kinase C epsilon (PKCc). Subsequently, PKC€ protein levels were also shown to be up-regulated in inflamed intestinal tissue from TNBS-treated rats. This was associated with increased NFlcB activation and IKBa protein levels, increases that were absent in non~inflamed tissue from TNBS-treated rats. In addition, IKB~ and Bcl-3 protein levels did not differ between inflamed and non-inflamed tissues, although they did vary with intestinal region. In conclusion, this study shows that abnormal NFKB activation and IKBa expression occurs in CD, and also suggests increased NFKB activation IKBa expression can coexist within inflamed intestinal tissue. In addition, the IEC line Caco-2 is shown to be relatively unresponsive to NFKB activation. In the macrophage cell line, RAW 264.7, PKC£ is involved in NFKB-mediated gene expression, and PKC£ protein levels are increased in the inflamed, TNBS-treated, intestine.

The consumption of dietary supplements such as flavonoids may reduce risk of many civilization diseases. Flavonoids are able to modulate the activity of cytochromes P450 (CYPs), xenobiotic-metabolising phase I enzymes of biotransformation that are involved in the activation and detoxification of food-derived carcinogens. Inhibition of CYP activities by flavonoids has been extensively studied because of their potential use as agents blocking the initiation stage of carcinogenesis. On the other hand, flavonoids have been shown to enhance the activation of carcinogens and/or influence their metabolism via induction of specific CYPs. In the first part of this study, flavonoids dihydromyricetin and α-napthoflavone were explored for their possible effects on CYP1A1 expression and activity when administered in combination with carcinogen benzo[a]pyrene (BaP). For this purpose, liver, small intestine and colon microsomal fractions were isolated from treated rats and induction of CYP1A1 was evaluated by immunodetection and EROD activity measurements. In liver and small intestine, all combinations of BaP and flavonoids led to strong induction of CYP1A1 expression. Moreover, the CYP1A1 protein levels were almost identical to levels observed when the rats were treated with BaP alone. However, in comparison...

Doctor of Philosophy / Department of Animal Sciences and Industry / Evan C. Titgemeyer / Previous data suggest that greater amounts of postruminal protein increase small intestinal starch digestion in cattle. Duodenally and ileally cannulated steers were used in 5 studies to measure responses in small intestinal starch digestion to amino acids (AA) or casein. Flows of starch to the ileum from the diet were small. Small intestinal starch digestibility was 34.0% when raw cornstarch was continuously infused into the duodenum. Infusion of casein linearly increased (P ≤ 0.05) small intestinal starch digestibility, and small intestinal starch digestion adapted to infusion of casein in 6 d. Ethanol-soluble starch and unpolymerized glucose flowing to the ileum increased linearly (P ≤ 0.05) with increasing infusion of casein. Plasma cholecystokinin was not affected by casein infusion, but circulating levels of glucose increased linearly (P ≤ 0.05). In another study, 5 steers were fed a low-starch diet and provided continuous duodenal infusion of raw cornstarch in combination with AA or casein in order to measure response of small intestinal starch digestion. Duodenal infusion of casein increased (P ≤ 0.05) small intestinal starch digestion. When a mixture of AA with a profile similar to casein (CASAA) was infused, small intestinal starch digestion was similar (P = 0.30) to casein infusion. Infusion of only non-essential AA tended to increase (P = 0.14) small intestinal starch digestion relative to control; however, infusion of essential AA alone did not affect (P = 0.84) small intestinal starch digestion. Additionally, infusion of casein or essential AA increased ileal flows of ethanol-soluble starch, but non-essential AA alone were not different than the negative control. Duodenal infusion of Glu increased (P ≤ 0.05) small intestinal starch digestion, whereas a mixture of Phe, Trp, and Met (PTM) did not. Neither Glu nor PTM increased ileal flow of ethanol-soluble starch, but Glu and PTM provided together tended (P = 0.07) to increase ileal flows of ethanol-soluble starch. Our data suggest that Glu alone can increase small intestinal starch digestion in cattle similar to casein, but increases in small intestinal starch digestion in response to Glu are not associated with an increase in ileal flows of ethanol-soluble starch.

Les plus grandes interfaces avec l'environnement extérieur sont la peau, et les muqueuses gastro-intestinales. Ces barrières, sont constamment menacées par des attaques physico-chimiques ou par des tentatives d'invasion de micro-organismes. Les phagocytes mononucléés qui comprennent les DCs, les monocytes et les macrophages et sont issus de la lignée myéloïde possèdent des propriétés distinctes de phagocytose de pathogènes et de cellules apoptotiques, d'apprêtement des antigènes et de présentation de ces derniers aux lymphocytes T. d'activation. La distinction de ces différentes cellules est un enjeu majeur pour la compréhension des mécanismes de la réponse immune et pour sa modulation dans des buts thérapeutiques. En utilisant des marqueurs cellulaires Ly-6C, CD64 et ainsi que le fait que les monocytes dépendent du récepteur de chimiomokine CCR2 pour émigrer de la moelle osseuse et les DCs de l'engagement du récepteur Flt3, nous avons montré pour la première fois qu'il existe dans la peau et l'intestin une cascade de différenciation qui conduit à des monocytes et des macrophages tissulaires et est distincte de celle donnant naissance aux DCs. Nous avons ensuite étudié le comportement de ces cellules dans une inflammation stérile dans la peau médiée par le DNFB (dinitrofluorobenzène) et dans une maladie inflammatoire de l'intestin (IBD) et montré que leurs capacités de migration vers les ganglions lymphatiques et de présentation antigénique à des lymphocytes T sont dépendantes du modèle utilisé. Cette déconvolution des populations tissulaires de cellules monuclées nous permet ainsi de disséquer le rôle de chacun de ces acteurs lors de la réponse immune. / The skin and the gastrointestinal mucosa that are the largest interfaces with the external environment. These barriers are the guardians of the body's integrity and are constantly threatened by physicochemical or microorganisms attacks. They have a dense network of effector cells dedicated to the defense of the body. Among them, mononuclear phagocytes which include DCs, monocytes and macrophages are all derived from the myeloid lineage and possess distinct properties of pathogens and apoptotic cells phagocytosis, antigens processing and presentation to T cells. However, DCs, monocytes and macrophages share common ancestry and functions and are hard to differentiate from each other in tissues and lymphoid organs. The distinction of these cells is a major challenge for understanding immune response's mechanisms and its modulation for therapeutic purposes.Using Ly-6C, CD64 and CCR2 as cell markers, as well as the CCR2 dependent emigration from bone marrow of monocytes and DCs dependency to Flt3-L, we have shown for the first time a cascade of monocytes differentiation, and separate populations of tissue monocytes, macrophages and DCs within the skin and the intestine. We then studied the behavior of these cells in a sterile skin inflammation mediated by DNFB (dinitrofluorobenzène) and in an inflammatory bowel disease (IBD) and showed that their ability to migrate to lymph nodes and to present antigens to naïve T lymphocytes are model dependent. Disentangling those tissue populations allows us to dissect the role of each of these actors in the immune response.

As células dendriticas (DC) da lamina própria constituem um grupo heterogêneo de células essenciais na homeostase do intestino. Nos últimos anos, estudos vem mostrando participação do complexo mTORC2 na regulação de células da imunidade inata. Desta forma, usando animais deficientes em mTORC2 nas DCs nos investigamos o papel deste complexo sob a funcoes das DCs num modelo de colite ulcerativa. Observamos que a ausência de mTORC2 nas DCs diminui a inflamação intestinal e prejudica a migração dessas células para os linfonodos. Consequentemente, estes animais também apresentam redução no numero de linfócitos Th17, Treg e T CD8&#43IFNg&#43. Estes resultados parecem ser independentes da composição da microbiota intestinal. In vitro, células dendriticas derivadas da medula ossea (BMDCs) deficientes em mTORC2 não mostraram deficiência na regulação positiva de CD80 e MHC-II quando estimuladas, mas mostraram menor produção de TNF-a e IL-6. Estas células também apresentaram menor capacidade na diferenciação de Th17. Nossos resultados indicam que mTORC2 participa na regulação das funções das DCs, especialmente na migração e produção de citocinas pos estímulos lnflamatorios. / Dendritic cells from lamina propria encompass a heterogeneous group of cells which play a key role in intestinal homeostasis. In the past few years, it has been shown the participation of mTORC2 in regulating innate immune cells. In our study, we used mTORC- deficient DCs to investigate the role of this complex in DCs functions in ulcerative colitis model. Absence of mTORC2 in DCs reduces the intestinal inflammation, impairs DCs migration which in turn decreases the number of Th17, Tregs and CD8&#43IFNg&#43 T cells. These results seem to be independent of gut microbiota composition. Our in vitro results showed that bone marrow-derived DCs (BMDCs) deficient in mTORC2 can upregulate CD80 and MHC-II after stimulus but present decreased production of TNF-a and IL-6. Those cells also showed deficiency in Th17 differentiation. Our results suggest that mTORC2 play pivotal role in DCs functions highlighting migration and cytokine production.

Orientador: Juliany Gomes Quitzan / Resumo: Devido à alta incidência de cirurgias gastrointestinais nas diferentes espécies, incluindo o homem, diversas técnicas e materiais são continuamente desenvolvidos visando otimizar os procedimentos e diminuir as principais complicações observadas, dentre elas deiscência, extravasamento de conteúdo, aderências, peritonite e sepse. O fio farpado é uma recente opção de sutura, que beneficia especialmente as modalidades cirúrgicas laparoscópicas ou videoassistidas, pois a dispensabilidade de nós facilita a sutura e diminui o tempo cirúrgico. Ainda, as farpas ao redor do fio permitem melhor adesão ao tecido e distribuição uniforme da tensão. Com o objetivo de comparar três tipos suturas realizadas com o fios absorvíveis monofilamentares, farpado ou liso, em relação ao tempo de sutura, escore de aderência, resistência ao teste de ruptura e avaliação histológica, foram executadas enterorrafias em jejuno de 12 suínos (sutura farpada, sutura contínua e isolada simples). Os animais foram randomizados em três grupos (n=4), referindo-se aos dias pós-operatórios para coleta das amostras (G3, G10 e G21). Houve diferença entre as médias dos tempos de execução, quando comparadas as suturas farpada (274,33±70,48) e contínua (350,41±87,25), em relação à isolada (469,00±109,72). Comparando-se os três padrões, nos diferentes momentos, os escores de aderência e as pressões aferidas no teste de ruptura não apresentaram diferença significativa, assim como a análise histopatológica, onde não foi obser... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Due to the high incidence of gastrointestinal surgeries in many species including humans, several techniques and materials have been continuously investigated to improving the procedures towards to avoid the most common complications, including dehiscence, leakage of contents, adhesions, peritonitis and sepsis. Barbed suture has recently come under focus for laparoscopic or video-assisted procedures, once it is a knotless suture and makes the suturing time shorter. In addition, the barbs around the suture promotes better adhesion to the tissue and uniform distribution of the tension. To compare three types of sutures performed with monofilament conventional or barbed suture, both absorbable, in relation to suture time, adhesion score, burst pressure and histology, jejunal sutures were performed in 12 pigs (barbed suture, continuous suture and simple interrupted). The groups (n = 4) were randomized, referring to the postoperative days for final evaluation (G3, G10 and G21). There was a difference on suturing time, when compared barbed (274.33 ± 70.48) and continuous (350.41 ± 87.25) to the interrupted pattern (469.00 ± 109.72). Among the 3 techniques at G3, 10 or 21, adherence scores and bursting pressure were not significantly different as well as histology evaluation, since no difference could be detected, in the grade of inflammation, fibrosis, tissue alignment, necrosis and neovascularization, at the suturing sites. No complications due to suture failures were observed. Ba... (Complete abstract click electronic access below) / Mestre

Estudo quantitativo, exploratório, descritivo e transversal, com objetivo de avaliar o manejo do intestino neurogênico e sua relação com a qualidade de vida (QV), satisfação e estilo de vida em pessoas com lesão medular traumática (LMT). Seguidos os preceitos éticos, a amostra foi composta por 81 adultos com LMT, de dois centros de reabilitação dos estados de São Paulo e Santa Catarina. Os dados foram coletados a partir de cinco instrumentos previamente validados (questionários sociodemográfico e de caracterização da LMT, Índice de Tratamento do Intestino e da Bexiga (BBTI) e dois data sets de função intestinal e QV desenvolvidos pela International Spinal Cord Society (ISCoS)). Foram realizadas análises descritivas e correlacionais. A maioria dos participantes era do sexo masculino, jovens, com idade média de 36 anos no momento da lesão, baixa escolaridade, beneficiários da previdência social, com baixa renda familiar (até três salários mínimos) e com a LMT no nível torácico. As principais causas da LMT foram acidentes de trânsito e queda, o tempo médio da LMT era de 4,7 anos. Os participantes utilizavam principalmente medidas conservadoras (massagem abdominal, manobra de Valsalva e estímulo dígito-anal) como método para esvaziamento intestinal. Cerca de 90% necessitava de até 30 minutos para o manejo intestinal, e tinha frequência de defecação acima de duas vezes por semana. Aproximadamente metade dos participantes relatou incontinência fecal. A independência para o cuidado intestinal não foi associada ao nível da LMT, no entanto esteve associada à satisfação com a rotina de cuidados intestinais e ao tempo da LMT, assim quanto maior o tempo de lesão, maior foi a independência para o manejo intestinal. Aproximadamente 40% e 35% referiram alterar suas rotinas diárias devido à incontinência fecal e constipação, respectivamente. O impacto na QV devido aos problemas intestinais foi relatado por 77,8% da amostra. Não houve diferença estatisticamente significativa entre QV geral, com a saúde psicológica e com a saúde física (p>0,05). A QV geral esteve associada ao tempo de LMT, e foi menor entre as pessoas que tiveram que alterar suas rotinas devido a perdas fecais e entre aquelas que precisavam utilizar fraldas. As pessoas satisfeitas com suas rotinas de cuidado intestinal apresentaram maior QV geral e maior satisfação com a saúde psicológica. Ainda há que se progredir na reabilitação intestinal das pessoas com LMT, desenvolvendo estratégias de capacitação para o manejo intestinal, com a finalidade de reduzir os fatores que impedem ou dificultam a pessoa com LMT a retornar para suas atividades sociais e laborais, acarretando prejuízos na vida pessoal e para a economia do país já que a maioria dessas pessoas era economicamente ativa e agora dependem da previdência social e dos serviços de saúde com mais frequência. Os achados deste estudo podem subsidiar a prática clínica nos serviços de reabilitação, considerando que um programa de reabilitação precocemente iniciado e individualmente planejado pode auxiliar a pessoa com LMT a ter um funcionamento intestinal adequado e consequentemente uma melhor QV, satisfação e estilo de vida / Quantitative, exploratory, descriptive and transversal study, with the aim to assess the management of the neurogenic intestine and its relationship with the quality of life (QOL), satisfaction and lifestyle on people with traumatic spinal cord injury (TSCI). In accordance with the ethical requirements, the sample was composed by 81 adults with TSCI, from two rehabilitation centers from the states of Sao Paulo and Santa Catarina. The data was collected from five previously validated instruments (socio-demographic questionnaires and of characterization of TSCI, Index of Bladder and Intestine Treatment (BBTI) and two data sets of intestinal function and QOL developed by the International Spinal Cord Society (ISCoS)). Descriptive and correlational analyses were carried out. Most of the participants were male, young, with an average age of 36 years at the time of the injury, low schooling, and social security beneficiaries with low family income (up to three minimum wages) and with the TSCI at thoracic level. The main causes of the TSCI were traffic accidents and falls, the average time TSCI was of 4.7 years. Participants used mainly conservative measures (abdominal massage, Valsalva maneuver and digital anal stimulus) as a method for bowel emptying. Around 90% needed up to 30 minutes for the intestinal management, and had defecation frequency above twice a week. Approximately half of the participants reported fecal incontinence. Intestinal care independence was not associated with the level of the TSCI, however it was associated with satisfaction with bowel care routine and to the time of TSCI, so the longer the time of injury, the greater the independence for the bowel management was. Approximately 40% and 35% reported altering their daily routines due to fecal incontinence and constipation, respectively. The impact on the QV due to intestinal problems was reported by 77, 8% of the sample. There was no significant statistical difference between general QOL with psychological health and physical health (p>0,05). General QOL was associated with the time of TSCI and was lower among the people who had to alter their routines due to fecal losses and among those who needed to use diapers. The people satisfied with their routines of intestinal care presented higher general QOL and higher satisfaction with their psychological health. There is still progress to be made in the intestinal rehabilitation of people with TSCI, developing training strategies for the intestinal management, with the purpose to reduce the factors that prevent people with TSCI to return to their social and labor activities, causing losses in personal life and for the country\'s economy since most of these people were economically active and now depend on social welfare and health services more frequently. The findings of thid study can provide clinical practice in rehabilitation services, considering that a rehabilitation program prematurely launched and individually planned can assist the person with TSCI to have proper bowel function and consequently a better QOL, satisfaction and lifestyle

Quarenta e duas vacas Holandesas, em gestação e multíparas, e os respectivos bezerros recém-nascidos foram utilizados para determinar se as concentrações de IGF-I no colostro e secreções lácteas podem ser alteradas em resposta à mudanças na concentração sérica de IGF-I pré-parto, avaliar comparativamente a flutuação sérica pré-parto de IGF-I em relação a IgG, bem como as flutuações séricas de IGF-I e IgG nos bezerros na primeira semana de vida e, adicionalmente, alterações na mucosa intestinal dos mesmos. As vacas foram distribuídas ao acaso em dois grupos de 21 animais, o grupo tratado recebeu 500 mg de somatotropina bovina recombinante (rbST) e o grupo controle recebeu injeções de vitamina E. Ambos tratamentos iniciaram 35 dias pré-parto e foram administrados em intervalos de 14 dias. Semanalmente, foi observado o escore corporal e foram coletadas amostras de sangue até a data de parição. Foram coletadas amostras do colostro e das secreções lácteas, diariamente, por sete dias pós-parto. Os bezerros recém-nascidos foram distribuídos ao acaso, em um arranjo fatorial 2X3, correspondendo ao tratamento das mães (controle ou rbST) e às datas de abate (ao nascimento, aos dois dias de idade e aos sete dias de idade). Diariamente, foram coletadas amostras de sangue até a data de abate. Para as análises estereológicas amostras foram coletadas de cinco regiões do intestino delgado. O escore corporal e a concentração sérica de ácidos graxos não-esterificados não diferiram entre os grupos durante todo o período experimental (P>0,05). Os grupos rbST e controle apresentaram diferenças significativas quanto às concentrações séricas de IGF-I na segunda e quarta semanas após o início do período experimental (P<0,05), em resposta às aplicações do rbST, no entanto não foram encontradas diferenças entre os tratamentos no parto (P>0,05). A concentração de IGF-I foi superior no colostro das vacas tratadas com rbST (P<0,05), não diferindo nas secreções subseqüentes. As concentrações séricas de IgG não diferiram entre os tratamentos durante todo o período experimental, bem como as concentrações de IgG do colostro e demais secreções lácteas (P>0,05). Não foram encontradas diferenças entre os tratamentos nas concentrações séricas de IGF-I dos bezerros até o sétimo dia de vida, como também não foram encontradas diferenças entre as concentrações séricas de IgG nos bezerros após 24 horas de vida (P>0,05). O peso dos órgãos e o volume parcial (Vv) da mucosa absortiva não diferiram entre os tratamentos nas três datas de abate, observando-se somente diferenças significativas entre as datas de abate. A porção do jejuno médio apresentou maior Vv em relação aos outros segmentos do intestino delgado ao nascimento e aos sete dias de vida. A aplicação de rbST no período seco influenciou somente a concentração de IGF-I no colostro, não alterando as concentrações séricas nos bezerros até o sétimo dia de vida, como também o Vv da mucosa absortiva do intestino delgado. / Forty-two Holstein cows, in gestation and multiparous, and their newborn calves were used to evaluate if insulin-like growth factor-I (IGF-I) in colostrum and subsequent mammary secretions could be influenced by changes in blood serum IGF-I and compare its temporal changes with the serum immunoglobulin G (IgG) in pre-partum period, as well as the temporal changes in serum IGF-I and IgG in newborn calves during the first week of life, and also, alterations in its intestinal mucosa. Cows were randomly assigned in two groups of twenty-one animals, treated group was injected 500 mg of recombinant bovine somatotropin (rbST), and the control group received vitamin E injection. Both treatments started 35 days pre-partum and were administered every 14 days until partum. Weekly body condition scores were measured and blood was collected until partum. Colostrum and mammary secretions were collected daily for seven days pos-partum. Newborn calves were randomly assigned to a 2X3 factorial arrangement, which the factors depended on mother’s treatment (control and rbST) and slaughter date (just after birth, two days of life and seven days of life). Blood was collected daily until slaughter. For stereological analysis samples were taken from five sites from small intestine. Body condition scores and nonesterified fatty acid concentration didn't differ between the groups during the experimental period (P>0,05). Groups differed about serum IGF-I during pre-partum (P<0,05), which showed differences on second and fourth weeks of experimental period in response to rbST administration. However, no treatment differences were found at partum (P>0,05). IGF-I concentration was higher in colostrum of cows treated with rbST (P<0,05), but it didn't differ in subsequent mammary secretions. IgG serum concentration didn't differ between treatments during the experimental period, neither IgG concentration in colostrum and subsequent mammary secretions (P>0,05). No differences were found either in calves’ IGF-I levels from birth to seven days old or in IgG serum concentration after 24 hours of life (P>0,05). Organ weight and mucosa partial volume (Vv) didn't differ between treatments in the three slaughter dates, only differences among the salughter dates were observed. Segment from the medium jejunum showed higher Vv in relation to others segments at birth and seven days old. The administration of rbST during the dry period influenced only IGF-I concentration in colostrum, however colostrum IGF-I didn’t affect calves’ serum concentration up to seven days old, neither the mucosa Vv of small intestine.