The Puerto Rican coqui frog (Eleutherodactylus coqui) has been hypothesized to affect bird communities in Hawaii by 1) competing with insectivorous birds for prey, 2) providing prey for predatory birds, and 3) bolstering populations of non-native mammals by serving as prey. No previous studies have collected empirical data on these potential impacts. We investigated the impact of coquis on birds at two scales. For our first research question, we used stable isotope analyses to address whether three species of insectivorous bird, one native and two non-native, and coquis could compete for invertebrate prey. We found that the coquis overlapped in isotopic niche space with all three bird species, which suggests these species occupy a similar place in the food web. However, our Bayesian diet analysis suggests that coquis mostly feed on Acari, Amphipoda, and Blattodea (>90%), and only consume about 2% Araneae, the only diet source they share with birds. This result suggests that coquis do not heavily compete with these bird species for prey. For our second research question, we conducted avian point counts in coqui and non-coqui plots across 15 sites on the island of Hawaii. We modeled whether coqui presence or density explained patterns of insectivorous, vertebrate-preying, and native bird abundance. We estimated abundances of birds in coqui and non-coqui plots using hierarchical Bayesian N-mixture models with random effects. We tested whether habitat variables differed across coqui and non-coqui plots and whether coqui density was correlated with any habitat variable to more confidently attribute changes in bird abundance across coqui and non-coqui plots to the frogs. We found that coquis were associated with greater abundances of vertebrate-preying, generalist insectivorous, and non-native birds in Hawaii. Vertebrate-preying birds showed the strongest association, with a 0.97 probability of abundance being at least 50% higher in coqui plots. Native birds did not show differences in abundance across coqui and non-coqui plots. Because insectivorous bird and native bird abundance did not differ across coqui invasion fronts, our results suggest that coquis primarily affect Hawaiian birds by serving as a food resource for predatory birds, and not as competitors for invertebrate prey.

Zugl.: Freie Univ., Diss., 2003 / Dateiformat: zip, Dateien im PDF-Format

<p>In recent years, the use of alcohol and psychoactive drugs in combination withdriving has recieved increased attention. The lack of in-vehicle devices capable ofdetecting recent drug consumption and the difficulties associated with the breathbasedalcolocks in use today makes it interesting to investigate methods that areable to non-invasivelly measure analytes directly in the blood.</p><p>The assignment of this project, commissioned by Volvo Technology Corporationand Volvo Car Corporation, is to map substances that constitute a possible threatto traffic safety, identify suitable detection markers as a proof of administrationof these substances, and study possible non-invasive techniques to detect thesemarkers. The objective is to present for Volvo if and how to continue evaluatingand developing a non-invasive detection device.</p><p>The project has been carried out by performing an extensive literature study and averification experiment. From the literature review, a number of substances affectingdriving performance could be identified, and a metabolic study was performedfor each drug to map suitable biomarkers. Furthermore, two potential techniquesfor non-invasive detection, near-infrared Raman spectroscopy and near-infraredspectroscopy, were found and evaluated. The experiment was conducted usingnear-infrared Raman spectroscopy, with the aim of investigating the sensitivityand linearity of the method for ethanol detection.</p><p>Based on the theoretical evaluation, both near-infrared Raman spectroscopy andnear-infrared spectroscopy are expected to have potential for non-invasive detectionof ethanol. The experiment further proved the theoretical conclusionsmade for near-infrared Raman spectroscopy. However, neither of the techniquesis thought to have potential for drug detection.Altogether, we believe that non-invasive ethanol detection is possible, but suggestfurther experiments in order to determine which technique to be preferred.</p>

Thesis (M.S.)--Oregon State University, 2007. / Printout. Includes bibliographical references (leaves 73-81). Also available on the World Wide Web.

Thesis (doctoral)--Swedish University of Agricultural Sciences, 2007. / Thesis documentation sheet inserted. Appendix reproduces five papers and manuscripts, two co-authored with others. Includes bibliographical references. Also issued electronically via World Wide Web in PDF format; online version lacks appendix.

This study investigated the role of an invasive tall cane, Arundo donax L. (Arundo), in the riparian water cycle. Four 100 meter transects were arrayed perpendicular to the lower Rio Grande in southwest Texas. The first objective was to determine the primary water source for Arundo by using naturally occurring stable isotopes. Surface soil, river water, groundwater, precipitation and rhizome samples were collected every month during 2010 and 2011 growing seasons, which coincided with a major flood that saturated soils in the first year followed by extreme drought in the second year. The second objective was to characterize how Arundo water use varied with water availability gradients in the riparian zone. Leaf gas exchange and leaf delta13C were measured along potential moisture gradients. The third objective was to understand the interaction between groundwater and surface water, and whether Arundo water use affected daily groundwater fluctuations. The isotope ratio of rhizome water was consistent with shallow soil moisture uptake and with previous observations of a relatively shallow, fibrous root system. Floodwater from July 2010 persisted in the soil for at least a year despite a severe drought, and became the dominant water source for Arundo during much of the study period. Although the alluvial water table in this floodplain was shallow (< 6 m) and subject to changes in river level, groundwater seemed not to be an important source for Arundo, so long as the soil moisture was sufficient. In this study, Arundo was not found to experience soil moisture limitation, and the spatial variability of Arundo transpiration was not associated with any soil moisture availability gradients. Arundo was found to close its stomata in response to increasing vapor pressure deficit (VPD), causing declining transpiration rate and increasing leaf delta13C composition. Significant exchange between the river and the alluvial groundwater was reflected in the similarity of isotopic compositions and the high correlation between river and groundwater elevations. Cross correlation analysis showed that over 50% of the diurnal groundwater fluctuations were caused by river stage changes. Consistent with the above ecophysiological and stable isotope results, Arundo water use was not found to influence daily groundwater fluctuations.

The LRIG1 gene (leucine-rich repeats and immunoglobulin like domains-1) at chromosome 3p14 is a proposed tumour suppressor gene whose gene product negatively regulates various receptor tyrosine kinases. This function has been the basis for classifying LRIG1 as a potential tumour suppressor gene (TSG). The ERBB receptor family is important in malignant cellular functions such as proliferation, survival, adhesion, migration and differentiation. In breast cancer, amplification of the ERBB2 proto-oncogene is an important negative prognostic factor. The epidermal growth factor receptor (EGFR/ERBB1), is expressed in colorectal cancer and has been correlated to a worse prognosis. Until recently, immunohistochemical analysis of EGFR expression was used to select patients suitable for treatment with EGFR targeted antibodies. This thesis characterizes LRIG1 in breast and colorectal cancer to gain further knowledge of the gene and its expression. Also, the EGFR expression in metastases and the invasive margin of colorectal cancers was investigated to correlate changes to clinical factors. Breast cancer samples and matched normal tissues were evaluated for LRIG1 and the ERBB receptors at gene, RNA and protein levels. An increase in copy number of the LRIG1 gene was evident. Also, increased LRIG1 copy number was associated with high levels of ERBB2 mRNA. Another set of breast cancer tumours were analysed for LRIG1 by FISH analysis. The results were coherent with the previous results. To further analyze the correlation to ERBB2, tumours with LRIG1 increased copy number were analysed for ERBB2. The data showed that 89% of tumours with increased LRIG1 copy number were either ERBB2 amplified or had an increased copy number of ERBB2. To investigate LRIG1 and the EGFR in colorectal cancer, the gene and protein expression was analysed by several methods in tumours and corresponding normal tissues. There were no significant changes at gene level found, but at the protein level, both over- and under expression were seen. No evident correlation between LRIG1 and EGFR expression was detected. The ERBB receptor family expression in colorectal cancer tumours and corresponding metastases was investigated to explore if the expression was altered in the metastatic lesion. The results showed that the EGFR expression was lost in the corresponding metastases in 33% of the tumours and that the same percentage of tumours gained expression in the metastases. Co-expression of the ERBB family members was also analysed; there was a significant increase of ERBB3/ERBB4 co-expression in late stage tumours. EGFR expression at the invasive margin of colorectal cancers was analysed to clarify whether expression correlated to the patient’s prognosis. Significant correlation to survival and the presence of budding was seen. In conclusion, 34% of the breast cancer tumours studied had an increased copy number of LRIG1 with a significant co-incidental increase in ERBB2 copy number. This raises the question of a functional correlation between LRIG1 and ERBB2, a finding that might be of clinical importance. The studies of EGFR and the ERBB receptors in colorectal cancer reflect the heterogeneity of EGFR expression in tumours. In addition, these findings suggest that survival of the patients correlates to an increasing EGFR expression at the invasive margin.

In recent years, the use of alcohol and psychoactive drugs in combination withdriving has recieved increased attention. The lack of in-vehicle devices capable ofdetecting recent drug consumption and the difficulties associated with the breathbasedalcolocks in use today makes it interesting to investigate methods that areable to non-invasivelly measure analytes directly in the blood. The assignment of this project, commissioned by Volvo Technology Corporationand Volvo Car Corporation, is to map substances that constitute a possible threatto traffic safety, identify suitable detection markers as a proof of administrationof these substances, and study possible non-invasive techniques to detect thesemarkers. The objective is to present for Volvo if and how to continue evaluatingand developing a non-invasive detection device. The project has been carried out by performing an extensive literature study and averification experiment. From the literature review, a number of substances affectingdriving performance could be identified, and a metabolic study was performedfor each drug to map suitable biomarkers. Furthermore, two potential techniquesfor non-invasive detection, near-infrared Raman spectroscopy and near-infraredspectroscopy, were found and evaluated. The experiment was conducted usingnear-infrared Raman spectroscopy, with the aim of investigating the sensitivityand linearity of the method for ethanol detection. Based on the theoretical evaluation, both near-infrared Raman spectroscopy andnear-infrared spectroscopy are expected to have potential for non-invasive detectionof ethanol. The experiment further proved the theoretical conclusionsmade for near-infrared Raman spectroscopy. However, neither of the techniquesis thought to have potential for drug detection.Altogether, we believe that non-invasive ethanol detection is possible, but suggestfurther experiments in order to determine which technique to be preferred.

Integrating mutualism into the study of ecological communities is likely to be critical to understanding community dynamics and to predict the consequences of anthropogenic changes to ecosystems. Invasive species are among the greatest of these threats to global biodiversity. Throughout the Pacific, the invasive ant Anoplolepis gracilipes associates mutualistically with Morinda citrifolia , a native plant with extrafloral nectaries (EFN). I tested the hypothesis that these interactions can mediate invader impacts Anoplolepis gracilipes abundances were positively correlated with the dominance of EFN-bearing plants per site and negatively correlated with the species richness of native ants. Additionally, A. gracilipes displayed a higher magnitude of responses to nectar than other dominant ants. Mutualisms also had significant impacts on the structure of arthropod communities. These effects were strongest when A. gracilipes dominated local ant assemblages. These results suggest that novel mutualisms between invasive and native species can facilitate the impacts of invasions on communities.

Invasive species are one of many important environmental issues facing Canadians today. A great deal of research has explored both the scientific and social aspects of invasive species. However, the cumulative research has not yet thoroughly explored people’s thoughts and feelings about, or perceptions of, invasive species and the influence these may have on management of, or policy decisions regarding, invasive species. This thesis research project was designed to assess the attitudes and perceptions that high school students have towards invasive species and to determine to what extent learning about invasive species alters their connection with nature. The study group was comprised of students from four Grade 11 Environmental Science classes from three high schools within the city of Guelph, Ontario. The students received regular classroom instruction from their teachers covering the course content, including invasive species. Students also made weekly visits to a local nature centre, providing them with hands-on learning experiences related to the course content. Students responded to an 80-question survey that assessed their knowledge of local invasive species and attitudes towards them as well as students’ connection to nature. Their connection to nature was assessed using a modified version of the Connectedness to Nature Scale (Mayer & Frantz, 2004). The surveys were administered by the classroom teachers in October, 2010, prior to the presentation of instructional material covering invasive species and then again in December, 2010 when the presentation of instructional material concerning invasive species was complete. The results showed that the students’ knowledge of invasive species upon entering the course was quite low and each of the classes witnessed a significant increase in knowledge. The survey results did not reveal any change to the students’ connection to nature; however, they did reveal three underlying themes in students’ attitudes towards invasive species: a concern about invasive species as a threat or problem; feelings of acceptance towards invasive species; and feelings of anxiety about invasive species. These attitudes remained relatively unchanged after the course.