In my diploma thesis, there was created the summary of solar construcion, toys and another elements, which are available on the market. At first some construction were selected, than there were the experiments were drawn up and at the end the experiments were done by students dutiny the lessons.There is also a short movie including these experiment. The diploma thesis contains my own design of solar construction, the description of its manufactory and its use in teaching physics. There is also the description of accompanying website. On this website i tis possible to download the wokrsheets. These worksheets were tested dutiny the teaching and later than at work evalueted.

A utilização de Inibidores da Tirosino Quinase (ITQ) alterou drasticamente a expectativa de vida do paciente com Leucemia Mielóide Crônica (LMC) e o monitoramento da expressão do oncogene BCR-ABL1 tornou-se um fator prognóstico fundamental para avaliação da resposta ao tratamento. Atualmente, a necessidade de desenvolvimento de metodologias moleculares que facilitem a quantificação rápida, barata e sensível, associada à detecção precoce de baixos níveis de BCR-ABL1, tem proporcionado o surgimento de diversos ensaios comerciais para monitoramento molecular. Entretanto, estes kits possuem uma variabilidade na sua composição, execução e parâmetros analíticos, principalmente com relação à sensibilidade, o que torna os resultados, muitas vezes, não comparáveis. Esse trabalho teve como objetivo revisar a literatura buscando identificar as diferentes opções comerciais disponíveis para o monitoramento do BCR-ABL1, além de comparar os resultados de dois destes ensaios com a metodologia de referência. A partir da revisão realizada, identificamos cinco kits comerciais como principais opções disponíveis para monitoramento de BCR-ABL1 na LMC: GeneXpert® BCR-ABL Assay (Cepheid), Ipsogen® BCR-ABL1 Mbcr Fusion Quant Kit (QIAGEN), BCR-ABL1 Quant RUO™ Assay (Asuragen), LightCycler® t(9;22) Quantification Kit (Roche Molecular Biochemicals) e ODK-1201 (Otsuka Pharmaceutical Co. Ltd.). Posteriormente, comparamos os resultados e avaliamos o desempenho dos ensaios GeneXpert® BCR-ABL e do BCR-ABL1 Quant RUO™ com a metodologia de referência a partir de amostras de 60 pacientes com LMC em uso de ITQ. Identificamos uma concordância global ótima, com coeficientes de correlação de 0,97 (GeneXpert® BCR-ABL Assay) e 0,84 (BCR-ABL1 Quant RUO™ Assay). No entanto, na avaliação da concordância relacionada ao alcance ou não de uma Resposta Molecular Maior (RMM), o ensaio BCR-ABL1 Quant RUO™ apresentou melhores resultados, com uma menor discrepância para respostas moleculares profundas. A análise estratificada por subtipos de transcritos de BCR-ABL1 não mostrou diferença de desempenho entre os dois ensaios. A partir das análises comparativas realizadas e respectivas vantagens de cada teste, aliados aos dados obtidos a partir da revisão da literatura, sugere-se que o GeneXpert® BCR-ABL poderia ser utilizado como um teste primário, devido à rapidez do ensaio, enquanto o BCR-ABL1 Quant RUO™, por apresentar resultados associados a uma maior sensibilidade, poderia ser um teste secundário, a fim de confirmar resultados abaixo de uma RMM ou resultados não detectáveis. Fica evidente que a escolha de um ensaio comercial deve atender às necessidades de cada laboratório, mas que, fundamentalmente, esteja alinhada às recomendações internacionais de quantificação. / The use of tyrosine kinase inhibitors (TKIs) has drastically changed the life expectancy of patients with chronic myeloid leukemia (CML) and monitoring the expression of the BCR-ABL1 oncogene has become a key prognostic factor for assessing treatment response. The need to development molecular methodologies that facilitate fast, cheap and sensitive quantification associated with the early detection of low levels of BCR-ABL1 has led to the emergence of several commercial assays for molecular monitoring. However, these kits have variability in their composition, performance and analytical parameters, mainly in relation to the sensitivity, which makes the results often not comparable. This work aimed to review the literature in order to identify the different commercial options available for the monitoring of BCR-ABL1, in addition to comparing the results of two of these tests with the reference methodology. From the review, we identified five commercial kits as the main options available for monitoring BCR-ABL1 in the LMC: GeneXpert® BCR-ABL Assay (Cepheid), Ipsogen® BCR-ABL1 Mbcr Fusion Quant Kit (QIAGEN), BCR-ABL1 Quant RUO™ Assay (Asuragen), LightCycler® t (9; 22) Quantification Kit (Roche Molecular Biochemicals) and ODK-1201 (Otsuka Pharmaceutical Co. Ltd.). Subsequently, we compared the results and evaluated the performance of the GeneXpert® BCR-ABL and BCR-ABL1 Quant RUO™ with reference methodology from samples of 60 patients with CML using TKI. We identified an optimal overall agreement for the two trials, with correlation coefficients of 0.97 and 0.84, respectively. However, in the evaluation of the agreement related to the reach of a Major Molecular Response (MMR), the BCR-ABL1 Quant RUO™ assay presented better results, with a smaller discrepancy for deep molecular responses. Analysis stratified by subtypes of BCR-ABL1 transcripts showed no difference in performance between the two assays. From the comparative analyzes performed and the respective advantages of each test, allied to the data obtained from the literature review, it is suggested that GeneXpert® BCR-ABL assay could be used as a primary test, due to the rapidity of the assay, while the BCR-ABL1 Quant RUO™, for presenting results associated with increased sensitivity, could be a secondary test in order to confirm results below an MMR or undetected results. It is clear that the choice of a commercial assay should meet the needs of each laboratory, but that it is fundamentally in line with international quantification recommendations.

It has been reported that globally we have achieved Millennium Development Goal (MDG) Target 7C, to halve the proportion of the population without access to safe drinking water; however, there is a major flaw with this statement. While Target 7C calls for access to `safe' drinking water, what is actually being measured and reported is access to an `improved' water source. The World Health Organization (WHO) maintains that they must use this proxy measure because the methods for water quality testing are too expensive and logistically complicated, but by doing so, they may be over reporting safe water coverage. This was shown to be true in Tamatave, Madagascar, where thermotolerant coliforms were detected in water from a type of `improved' source, the Pitcher Pump system. This research looked at several parameters - Pitcher Pump system depth, sampling neighborhood, requirement of pump priming, frequency that the system was repaired, distance from on-site sanitation, and number of users - to see if they were influencing water quality. Of all the parameters tested, only priming was found to be significantly associated with the levels of thermotolerant coliforms detected (Fisher exact test p = 0.03). Using a Mann-Whitney U test, it was shown that the median thermotolerant coliform concentration was significantly higher in primed wells (41.3 cfu/100 ml) than unprimed wells (3.5) (p = 0.01 cfu/100 ml). A pilot study was conducted to look at only the effect of depth and to determine if a depth could be identified that could provide safe drinking water. The result of the pilot study showed that, while thermotolerant coliform concentration did decrease with increasing depth, even at the deepest well of 9.4 m, levels were still above 100 cfu/100 ml. Additional research was conducted to investigate the performance and cost of three test kits for both total coliform and Escherichia coli quantification for water quality analysis in developing countries. IDEXX Colilert Quanti-trays[reg] (Colilert), Micrology Laboratories Coliscan[reg] Membrane Filtration tests (Coliscan MF) and a modified method for 3-M PetrifilmTM Coliform/E. coli plates (modified 3-M) were compared with standard membrane filtration (standard MF) methods under a range of incubation temperature conditions (22.0, 35.0 and 44.5[deg]C). Each test method was also performed by inexperienced volunteers, with the results compared to those of an experienced technician. At non-standard temperatures, Coliscan MF proved to be the most accurate when compared to standard methods, with a significant difference with only total coliforms at 44.5[deg]C. Modified 3-M had the poorest correlation with standard MF over the range of temperatures tested, with significant differences noted for all the temperatures except for E. coli at 44.5[deg]C. Inexperienced university volunteers found Colilert easiest to use, but Coliscan MF produced E. coli results that were most similar to the experts. Coliscan MF was found to have the overall best performance and lowest cost in this study; however, it did produce high numbers of false positive results.

The purpose of this evaluation study was to compare students' perceptions of their science classroom environment when using science kits, textbooks or a combination of science kits, textbooks and teacher-created materials. This year-long study involved using a learning environment questionnaire, namely the My Class Inventory (MCI), interviews and observations to assess which of the three treatments leads to a more positive learning environment. Three questions investigated were whether (1) the learning environment can be reliably and validly assessed among Grade 3-5 students in Texas, (2) instruction using textbooks, science kits, or a combination of textbooks and science kits is more effective in terms of changes in student attitudes and learning environment perceptions, and (3) there are associations between student attitudes toward science classes and the classroom environment? Administrators and teachers in Texas are searching for ways to improve the scores received on standardized tests. For more than 40 years, research has shown that positive classroom environments can lead to improvement in achievement. Therefore 1 chose to investigate the above questions using a learning environments framework. This study was conducted in three urban elementary schools in North Texas. There were a total of 588 students in 28 classrooms with 16 different teachers involved in this research. The schools were similar in demographic features such as ethnicity and socioeconomic status. Analyses of data collected with the My Class Inventory (MCI) supported the instrument's factorial validity, internal consistency reliability, and ability to differentiate between the perceptions of students in different classrooms. / Also, simple correlation and multiple regression analyses indicated reasonably strong and positive associations between each classroom environment scale and the students' satisfaction. The Satisfaction scale was used as an outcome variable, following the lead of Majeed, Fraser and Aldridge (2002). Results h m the MCI, interviews and observations indicated that students preferred a more positive classroom environment in terns of Cohesiveness, Competition, and Friction. Importantly, the group of students using science kits experienced greater pretest-posttest changes in satisfaction and classroom cohesiveness than did either the textbook group of the combination group. This study supports previous research that combined qualitative and quantitative methods of data collection. Qualitative methods suggested that students preferred a more hands-on presentation of science lessons rather than a textbook presentation. This was suggested in interviews with students and teachers and by observations of students in their science classes. This research evaluated three educational methods to determine which instructional method would produce a more positive learning environment and student satisfaction. These results suggest that the utilization of science kits achieves this goal as measured by student satisfaction and cohesiveness.

Diss. (sammanfattning) Stockholm : Karol. inst., 2006. / Härtill 5 uppsatser.

A utilização de Inibidores da Tirosino Quinase (ITQ) alterou drasticamente a expectativa de vida do paciente com Leucemia Mielóide Crônica (LMC) e o monitoramento da expressão do oncogene BCR-ABL1 tornou-se um fator prognóstico fundamental para avaliação da resposta ao tratamento. Atualmente, a necessidade de desenvolvimento de metodologias moleculares que facilitem a quantificação rápida, barata e sensível, associada à detecção precoce de baixos níveis de BCR-ABL1, tem proporcionado o surgimento de diversos ensaios comerciais para monitoramento molecular. Entretanto, estes kits possuem uma variabilidade na sua composição, execução e parâmetros analíticos, principalmente com relação à sensibilidade, o que torna os resultados, muitas vezes, não comparáveis. Esse trabalho teve como objetivo revisar a literatura buscando identificar as diferentes opções comerciais disponíveis para o monitoramento do BCR-ABL1, além de comparar os resultados de dois destes ensaios com a metodologia de referência. A partir da revisão realizada, identificamos cinco kits comerciais como principais opções disponíveis para monitoramento de BCR-ABL1 na LMC: GeneXpert® BCR-ABL Assay (Cepheid), Ipsogen® BCR-ABL1 Mbcr Fusion Quant Kit (QIAGEN), BCR-ABL1 Quant RUO™ Assay (Asuragen), LightCycler® t(9;22) Quantification Kit (Roche Molecular Biochemicals) e ODK-1201 (Otsuka Pharmaceutical Co. Ltd.). Posteriormente, comparamos os resultados e avaliamos o desempenho dos ensaios GeneXpert® BCR-ABL e do BCR-ABL1 Quant RUO™ com a metodologia de referência a partir de amostras de 60 pacientes com LMC em uso de ITQ. Identificamos uma concordância global ótima, com coeficientes de correlação de 0,97 (GeneXpert® BCR-ABL Assay) e 0,84 (BCR-ABL1 Quant RUO™ Assay). No entanto, na avaliação da concordância relacionada ao alcance ou não de uma Resposta Molecular Maior (RMM), o ensaio BCR-ABL1 Quant RUO™ apresentou melhores resultados, com uma menor discrepância para respostas moleculares profundas. A análise estratificada por subtipos de transcritos de BCR-ABL1 não mostrou diferença de desempenho entre os dois ensaios. A partir das análises comparativas realizadas e respectivas vantagens de cada teste, aliados aos dados obtidos a partir da revisão da literatura, sugere-se que o GeneXpert® BCR-ABL poderia ser utilizado como um teste primário, devido à rapidez do ensaio, enquanto o BCR-ABL1 Quant RUO™, por apresentar resultados associados a uma maior sensibilidade, poderia ser um teste secundário, a fim de confirmar resultados abaixo de uma RMM ou resultados não detectáveis. Fica evidente que a escolha de um ensaio comercial deve atender às necessidades de cada laboratório, mas que, fundamentalmente, esteja alinhada às recomendações internacionais de quantificação. / The use of tyrosine kinase inhibitors (TKIs) has drastically changed the life expectancy of patients with chronic myeloid leukemia (CML) and monitoring the expression of the BCR-ABL1 oncogene has become a key prognostic factor for assessing treatment response. The need to development molecular methodologies that facilitate fast, cheap and sensitive quantification associated with the early detection of low levels of BCR-ABL1 has led to the emergence of several commercial assays for molecular monitoring. However, these kits have variability in their composition, performance and analytical parameters, mainly in relation to the sensitivity, which makes the results often not comparable. This work aimed to review the literature in order to identify the different commercial options available for the monitoring of BCR-ABL1, in addition to comparing the results of two of these tests with the reference methodology. From the review, we identified five commercial kits as the main options available for monitoring BCR-ABL1 in the LMC: GeneXpert® BCR-ABL Assay (Cepheid), Ipsogen® BCR-ABL1 Mbcr Fusion Quant Kit (QIAGEN), BCR-ABL1 Quant RUO™ Assay (Asuragen), LightCycler® t (9; 22) Quantification Kit (Roche Molecular Biochemicals) and ODK-1201 (Otsuka Pharmaceutical Co. Ltd.). Subsequently, we compared the results and evaluated the performance of the GeneXpert® BCR-ABL and BCR-ABL1 Quant RUO™ with reference methodology from samples of 60 patients with CML using TKI. We identified an optimal overall agreement for the two trials, with correlation coefficients of 0.97 and 0.84, respectively. However, in the evaluation of the agreement related to the reach of a Major Molecular Response (MMR), the BCR-ABL1 Quant RUO™ assay presented better results, with a smaller discrepancy for deep molecular responses. Analysis stratified by subtypes of BCR-ABL1 transcripts showed no difference in performance between the two assays. From the comparative analyzes performed and the respective advantages of each test, allied to the data obtained from the literature review, it is suggested that GeneXpert® BCR-ABL assay could be used as a primary test, due to the rapidity of the assay, while the BCR-ABL1 Quant RUO™, for presenting results associated with increased sensitivity, could be a secondary test in order to confirm results below an MMR or undetected results. It is clear that the choice of a commercial assay should meet the needs of each laboratory, but that it is fundamentally in line with international quantification recommendations.

This thesis describes the design and implementation of a building kit suitable for teaching how to work with Atmel AVR single-chip computer and its peripheral circuits. Included in the thesis are drafts of electrical schematics of each individual module of the building kit, drawings of the PCBs as well as propositions for sample tasks used in teaching this subject. These further encompass methodological and work papers. Due to it's simplicity and clarity the Bascom AVR was chosen as the programming language for all applications.

A utilização de Inibidores da Tirosino Quinase (ITQ) alterou drasticamente a expectativa de vida do paciente com Leucemia Mielóide Crônica (LMC) e o monitoramento da expressão do oncogene BCR-ABL1 tornou-se um fator prognóstico fundamental para avaliação da resposta ao tratamento. Atualmente, a necessidade de desenvolvimento de metodologias moleculares que facilitem a quantificação rápida, barata e sensível, associada à detecção precoce de baixos níveis de BCR-ABL1, tem proporcionado o surgimento de diversos ensaios comerciais para monitoramento molecular. Entretanto, estes kits possuem uma variabilidade na sua composição, execução e parâmetros analíticos, principalmente com relação à sensibilidade, o que torna os resultados, muitas vezes, não comparáveis. Esse trabalho teve como objetivo revisar a literatura buscando identificar as diferentes opções comerciais disponíveis para o monitoramento do BCR-ABL1, além de comparar os resultados de dois destes ensaios com a metodologia de referência. A partir da revisão realizada, identificamos cinco kits comerciais como principais opções disponíveis para monitoramento de BCR-ABL1 na LMC: GeneXpert® BCR-ABL Assay (Cepheid), Ipsogen® BCR-ABL1 Mbcr Fusion Quant Kit (QIAGEN), BCR-ABL1 Quant RUO™ Assay (Asuragen), LightCycler® t(9;22) Quantification Kit (Roche Molecular Biochemicals) e ODK-1201 (Otsuka Pharmaceutical Co. Ltd.). Posteriormente, comparamos os resultados e avaliamos o desempenho dos ensaios GeneXpert® BCR-ABL e do BCR-ABL1 Quant RUO™ com a metodologia de referência a partir de amostras de 60 pacientes com LMC em uso de ITQ. Identificamos uma concordância global ótima, com coeficientes de correlação de 0,97 (GeneXpert® BCR-ABL Assay) e 0,84 (BCR-ABL1 Quant RUO™ Assay). No entanto, na avaliação da concordância relacionada ao alcance ou não de uma Resposta Molecular Maior (RMM), o ensaio BCR-ABL1 Quant RUO™ apresentou melhores resultados, com uma menor discrepância para respostas moleculares profundas. A análise estratificada por subtipos de transcritos de BCR-ABL1 não mostrou diferença de desempenho entre os dois ensaios. A partir das análises comparativas realizadas e respectivas vantagens de cada teste, aliados aos dados obtidos a partir da revisão da literatura, sugere-se que o GeneXpert® BCR-ABL poderia ser utilizado como um teste primário, devido à rapidez do ensaio, enquanto o BCR-ABL1 Quant RUO™, por apresentar resultados associados a uma maior sensibilidade, poderia ser um teste secundário, a fim de confirmar resultados abaixo de uma RMM ou resultados não detectáveis. Fica evidente que a escolha de um ensaio comercial deve atender às necessidades de cada laboratório, mas que, fundamentalmente, esteja alinhada às recomendações internacionais de quantificação. / The use of tyrosine kinase inhibitors (TKIs) has drastically changed the life expectancy of patients with chronic myeloid leukemia (CML) and monitoring the expression of the BCR-ABL1 oncogene has become a key prognostic factor for assessing treatment response. The need to development molecular methodologies that facilitate fast, cheap and sensitive quantification associated with the early detection of low levels of BCR-ABL1 has led to the emergence of several commercial assays for molecular monitoring. However, these kits have variability in their composition, performance and analytical parameters, mainly in relation to the sensitivity, which makes the results often not comparable. This work aimed to review the literature in order to identify the different commercial options available for the monitoring of BCR-ABL1, in addition to comparing the results of two of these tests with the reference methodology. From the review, we identified five commercial kits as the main options available for monitoring BCR-ABL1 in the LMC: GeneXpert® BCR-ABL Assay (Cepheid), Ipsogen® BCR-ABL1 Mbcr Fusion Quant Kit (QIAGEN), BCR-ABL1 Quant RUO™ Assay (Asuragen), LightCycler® t (9; 22) Quantification Kit (Roche Molecular Biochemicals) and ODK-1201 (Otsuka Pharmaceutical Co. Ltd.). Subsequently, we compared the results and evaluated the performance of the GeneXpert® BCR-ABL and BCR-ABL1 Quant RUO™ with reference methodology from samples of 60 patients with CML using TKI. We identified an optimal overall agreement for the two trials, with correlation coefficients of 0.97 and 0.84, respectively. However, in the evaluation of the agreement related to the reach of a Major Molecular Response (MMR), the BCR-ABL1 Quant RUO™ assay presented better results, with a smaller discrepancy for deep molecular responses. Analysis stratified by subtypes of BCR-ABL1 transcripts showed no difference in performance between the two assays. From the comparative analyzes performed and the respective advantages of each test, allied to the data obtained from the literature review, it is suggested that GeneXpert® BCR-ABL assay could be used as a primary test, due to the rapidity of the assay, while the BCR-ABL1 Quant RUO™, for presenting results associated with increased sensitivity, could be a secondary test in order to confirm results below an MMR or undetected results. It is clear that the choice of a commercial assay should meet the needs of each laboratory, but that it is fundamentally in line with international quantification recommendations.

There is an increasing demand for DNA analysis because of the sensitivity of the method and the ability to uniquely identify and distinguish individuals with a high degree of certainty. But this demand has led to huge backlogs in evidence lockers since the current DNA extraction protocols require long processing time. The DNA analysis procedure becomes more complicated when analyzing sexual assault casework samples where the evidence contains more than one contributor. Additional processing to separate different cell types in order to simplify the final data interpretation further contributes to the existing cumbersome protocols. The goal of the present project is to develop a rapid and efficient extraction method that permits selective digestion of mixtures. Selective recovery of male DNA was achieved with as little as 15 minutes lysis time upon exposure to high pressure under alkaline conditions. Pressure cycling technology (PCT) is carried out in a barocycler that has a small footprint and is semi-automated. Typically less than 10% male DNA is recovered using the standard extraction protocol for rape kits, almost seven times more male DNA was recovered from swabs using this novel method. Various parameters including instrument setting and buffer composition were optimized to achieve selective recovery of sperm DNA. Some developmental validation studies were also done to determine the efficiency of this method in processing samples exposed to various conditions that can affect the quality of the extraction and the final DNA profile. Easy to use interface, minimal manual interference and the ability to achieve high yields with simple reagents in a relatively short time make this an ideal method for potential application in analyzing sexual assault samples.

El presente trabajo de investigación plantea un estudio sobre la factibilidad de realizar un proyecto económicamente rentable respecto a la personalización de regalos para caballeros para ocasiones especiales en el mercado peruano debido a que se ha investigado sobre la dificultad permanente de elegir un obsequio para un hombre al no contar con muchas opciones a medida de los clientes en mercado nacional o regional. Nuestra ventaja diferencial es “Ser una marca especializada en el diseño, personalización y entrega de regalos para hombres, generando nuevas experiencias duraderas en ocasiones especiales; cuya propuesta realza tres preguntas: ¿Qué contiene esta caja? ¿Para qué será esta barreta?; y ¿Requerirá de mucha fuerza abrirla?”. Nuestra propuesta va dirigida al segmento: mujeres y hombres de los distritos de Lima Metropolitana, para quienes es importante regalar más que un obsequio; una experiencia original y personalizada en una ocasión especial. Para comprender mejor el mercado y al consumidor se empleó un cuestionario y las redes sociales para identificar factores críticos de éxito y fracaso que pueda tener la investigación en el mercado nacional y las acciones necesarias para que la viabilidad del mismo perdure y sea replicable en la región. Contaremos con una plataforma digital para la comercialización de los regalos personalizados y respecto al análisis financiero se concluye que la idea de negocios presentada es rentable, mostrando flujos de caja positivos a partir del segundo año y una Tasa de Retorno de 49.22% superior a lo mínimo esperado de un negocio de este rubro. / The present research project proposes a study on the feasibility of making an economically profitable project regarding the personalization of gifts for gentlemen for special occasions in the Peruvian market due to the fact that it has been investigated about the permanent difficulty of choosing a gift for a man. not have many options tailored to customers in national or regional market. Our differential advantage is "Being a brand specialized in the design, personalization and delivery of gifts for men, generating new lasting experiences on special occasions; whose proposal highlights three questions: What does this box contain? What will this bar be for? and Will it require a lot of force to open it? " Our proposal is aimed at the segment: women and men from the districts of Metropolitan Lima, for whom it is important to give more than a gift; An original and personalized experience on a special occasion. In order to better understand the market and the consumer, a questionnaire and social networks were used to identify critical factors of success and failure of the research in the national market and the actions necessary for its viability to be replicable in the region. We will have a digital platform for the marketing of personalized gifts and regarding financial analysis it is concluded that the business idea presented is profitable, showing positive cash flows from the second year and a Return Rate of 49.22% higher than the minimum expected from a business in this area. / Trabajo de investigación