The National Science Education Standards (1996) indicate that science education should include inquiry instruction. Many teachers still struggle with how to implement inquiry in their classrooms and a lack of high quality inquiry-based instructional materials has been posited as a hindrance. The purpose of this qualitative study was to observe the instructional practices of three elementary teachers when using an inquiry-based science kit program in their fourth grade classrooms. Teacher practices and their attitudes towards their preparedness to teach science with the support of the curricular program were examined. Data were collected through pre/post survey comparisons, observations, and a focus group session. Results indicated that these teachers' attitudes were positively impacted. Teachers' access to science kits provided resources which facilitated more inquiry experiences with their students; however, resources alone did not fully address teacher science content needs.

<p>I dag kan man oftast läsa om olika säkerhetshot och risker en datoranvändare måste tänka på för att inte ge någon utomstående möjlighet att komma åt känslig och/eller privat information. Här talas det om nya virus och nya typer av trojaner som sprids som epidemier över Internet, och i bland handlar det om ett spionprogram som följer med en nedladdad fil. Det är svårt att hålla reda på alla typer av skadlig kod som nämns fast med ökad förståelse ökar också chanserna för att klara sig från smitta. Det har visat sig att utvecklingen av skadlig kod är lika stark som den inom kommersiella mjukvaror. Från persondatorns uppkomst i början av 80-talet och fram till i dag, har utveckling skett i alla områden av den skadliga kod det handlar om strategi, syfte och framförallt ren kodkomplexitet.</p><p>Dagens ledande leverantör av operativsystem och webbläsare, Microsoft, lovar allt mer sofistikerade säkerhetslösningar varje gång en ny version av ett program släpps. Framförallt nämndes det i samband med lanseringen av Windows Vista att säkerheten var det som stod högst på listan.</p><p>Vi har tillsammans med WM-data i Stockholm tagit fram en programvara för fjärradministration av Windows. Huvudmålet var att med hjälp av våra baskunskaper i programmering skapa ett program för Windows XP och Windows Vista där en rad funktioner skulle kunna fjärrstyras utan att en användare vid den drabbade datorn upptäckte intrånget.</p><p>I denna rapport beskrivs utvecklingen av programvaran och de tester som gjorts på de båda operativsystemen. Vidare delas begreppet ”skadlig kod” upp i kategorierna virus, maskar, trojaner samt rootkits och förklaras mer ingående tillsammans med en historisk bild över hur utvecklingen av skadlig kod har sett ut.</p> / <p>In media today, you often read about different security threats and risks that one has to be aware of. Many things must be taken into consideration in order to maintain your integrity and information secrecy. It might be new virus outbreak, a new trojan or some kind of spy ware that undetected finds the way to your computer. It’s hard to keep track of all terms and types of malicious code, and with greater understanding, the risk of infection decreases. The development when it comes to malicious code is as strong as the one in commercial software development. From the 80’s until present day, every area in the development of malicious code has evolved, from strategy and purpose to the pure complexity of the code.</p><p>Microsoft, the worlds leading supplier of operating systems and web browsers, ensure us with every new release, that measures has been taken in order to enhance the security features. As the new operating system Windows Vista was released, spokesmen said that the security was now the highest priority.</p><p>We have, together with WM-data in Stockholm, developed software for remote administration of Windows. The objectives where by using our limited programming skills only, to come up with a program for Windows XP and Windows Vista, where a number of functions could be remotely executed without alerting a user at the infected computer.</p><p>This report describes the development of the software together with test results of execution on both operating systems. Further on, the report discusses different types of malicious code, such as viruses, worms, Trojans and root kits, together with a historical study of the development of malicious code.</p>

This diploma thesis deals with the use of robotic kits in teaching technical subject at high schools in the Czech Republic. The theoretical part describes the concepts of robotics and pedagogy. It deals with the history, types of robots, describes its components and provides an overview of the available robotic kits. It also examines the concepts and approaches in teaching, goals and activation methods. The results of research among students, alumni and teachers of high schools are summarized in the practical part. The outcome of this thesis is methodical instructions for teaching technical subjects, such as microprocessor technology and control systems, etc. using a robotic kit based on the Raspberry Pi. Elaborated tasks are: Hardware Hello World! LED, buzzer, switcher with LED, interrupt, motion detection, tracking a black line, keypad 4×4, fan speed control, steering the direction of rotation of DC motors, servo motor control, distance measurement, temperature measurement, display messages on the LCD screen, building a robotic rover and image acquisition from a webcam.

The goal of this paper is to suggest suitable senzoric subsystem of mobile robot. There are being analyzed robotic kits of different manufacturers. Their advantages and disadvantages in the theoretical way are being explained in this part and also their opportunities of use in the practical way. In the following parts the work is focused on senzoric subsystems of other manufacturers and again are desribed their advantages and disadvantages. On the last part there is shown own concept of senzoric subsystem. HW and SW implementation is described and there are shown testing data of the conception.

Thesis (MScMed)--Stellenbosch University, 2008. / ENGLISH ABSTRACT: Introduction: Flow cytometry has progressively replaced many traditional laboratory tests due to its greater accuracy, sensitivity and rapidity in the routine clinical settings especially clinical trails. It is a powerful tool for the measuring of chemical (the fluorochrome we add) and physical (size and complexity) characteristics of individual cells. As these instruments became major diagnostic and prognostic tools, the need for more advanced quality control, standardized procedures and proficiency testing programs increased as these instrumentations and their methodology evolve. Minor instrument settings can affect the reliability, reproducibility and sensitivity of the cytometer and should be monitored and documented in order to ensure identical conditions of measurement on a daily basis. This can be accomplished by following an Internal Quality Assurance (IQA) and/ or External Quality Assurance (EQA) program. Currently there are no such programs available in South Africa and poorer Africa countries. HIV is a global concern and the laboratories and clinics in these places are in need of such IQA programs to ensure quality of their instrumentation and accurate patient results. Quality assurance programs such as CD Chex® and UK Nequas are available but due to bad sample transport, leave the receiving laboratories with nightmares. It would be best if there was a laboratory in South Africa that could provide the surrounding laboratories with stabilized whole blood samples that can be utilized as IQA. The transport of these samples can be more efficient due to shorter distance and thus the temperature variations limited. Aims and Objectives: The aim of Chapter one is to familiarize the reader with general terminology and concepts of immunology. Chapter two describes in detail the impact stabilized whole blood had on clinical immunology concerning Quality Control and Quality Assurance. The objective of this study is to stabilize whole blood with a shelf life of greater than 30 days to serve as reference control material for South African Immunophenotyping. It is further an objective to use these in-house stabilized control samples for poorer African countries as Internal Quality Assurance reference material. It is a still further objective to stimulate various lymphocyte subsets to express activation antigens and then stabilize these cells for more specialized immunological test and can serve as a QC for those required samples. Study design: In Chapter three, the method currently used to stabilize whole blood was modified. The stability of different concentrations of a first stabilizing agent (Chromium Chloride hexahydrate) was investigated. Incubation periods and concentrations of paraformaldehyde as second stabilizing agent were investigated. Blood samples from healthy individuals (n=10) were stabilized and monitored for the routine HIV phenotypic surface antigens over a period of 40 days. These samples (n=10) were compared on the Becton Dickinson Biosciences (BD) FACSCalibur™ versus BD FACSCount™ instrumentation. Blood samples (n=3) were stabilized and monitored to identify phenotypic cell surface molecules for as long as possible. They were quantified on both flow cytrometric instruments. In addition, these stabilized samples (n=3) were investigated as control blood for calibration purposes on the BD FACSCount™ instrument. In Chapter four, lymphocytes were isolated and activated with various stimuli to express sufficient activation antigens such as CD25, CD69, HLA-DR and CD40 Ligand on the T helper cell surfaces. These activated antigens were analyzed on the BD FACSCalibur™ and further stabilized to serve as possible IQA samples in future. Results: In Chapter three, the ten individual stabilized samples had non-significant P values (P > 0.05) for CD3, CD4 and CD8 percentages and absolute values comparing day 3 until day 40. Comparing the BD FACSCalibur™ versus BD FACSCount™, resulted in a R2 = 0.9848 for CD4 absolute values and a R2 = 0.9636 for CD8 absolute values. Stabilized blood samples (n=3) were monitored for routine HIV phenotypic markers until day 84. The cells populations were easily identifiable and could be quantified on both BD FACSCalibur™ and BD FACSCount™ instruments. In Chapter four; for the activation study purposes, activated T helper lymphocytes expressed approximately 25 to 35% CD40 Ligand cell surface molecules. The stimulant of choice was Ionomycin at a 4μM concentration. Cells were incubated for four hours at 37 degree Celsius in a 5% CO2 environment. For CD69 surface expression, 6 hour incubation was optimum. The stimulus of choice in this case was 4μM Ionomycin which induced 84.21% CD69 expression in the test samples. For CD25 expression; 6 hour incubation with PHA resulted in approximately 43% of CD25 expression. For HLA-DR surface expression; 6 hour incubation with PHA resulted in approximately 43.32% of HLA-DR expression. Activated lymphocytes expressing CD40 Ligand showed stability until day 23. Activated Lymphocytes expressing CD69, CD25 and HLA-DR were stabilized in the same manner and stability could be achieved until day 16. Conclusion: This thesis was related to the preparation of control samples (IQA) designed to simulate whole blood having defined properties in clinical laboratory situations. In future kits can be developed with a low, medium and high control sample for the various immunological phenotypic determinants. Another kit can be compiled where various activation markers can be identified, quantified with a “zero”, low and high control. These whole blood IQA kits and “activation IQA kits” can be implemented for training of newly qualified staff, competency testing of staff, method development, software testing, panel settings and instrument setting testing. Control samples ideally must have a number of properties in order to be effective. For instance stability during storage times, preferably lasting more than a few weeks, reproducibility and ease of handling. These will provide the information on day-to-day variation of the technique or equipment which will enhance accuracy and improve patient care. / AFRIKAANSE OPSOMMING: Inleiding: Vloeisitometrie tegnologie het verskeie tradisionele laboratorium toetse vervang as gevolg van beter akuraadheid, sensitiwiteit en vinniger beskikbaarheid van resultate in ‘n kliniese omgewing, veral kliniese proewe. Vloeisitometrie is ‘n kragtige tegniek om chemiese (fluorokroom byvoeging) en fisiese (sel grote en kompleksiteit) karakter eienskappe van individuele selle te meet. Met die toename in gebruik en gewildheid van hiedie instrumente, neem die behoefde toe vir gevorderde kwaliteit kontroles, gestandardiseerde prosedures, met profesionele toets programme tesame met metode ontwikkeling. Klein verstellings aan instrument parameters beinvloed die betroubaarheid, herhaalbaarheid en sensitiwiteit van ‘n sitometer en moet gemonitor (en dokumenteer) word om identiese kondisies van leesings op ‘n daaglikse basis te verseker. Dit kan bereik word deur in te skakel met ‘n interne kwaliteits versekerings program [IQA: “Internal Quality Control”] en/of ‘n eksterne kwaliteits versekerings program [EQA: “External Quality Control”] te volg. Op die oomblik is daar geen sulke kwaliteits versekerings programme in Suid Afrika en/of in die verarmende Afrika lande beskikbaar nie. MIV is ‘n wêreldwye bekommernis en laboratoriums en klinieke in hierdie gedeeltes van die land verlang ‘n dringende behoefdte vir sulke “IQA” programme om kwaliteit van instrumentasie en akkurate pasiënt resultate te verseker wat tot beter behandeling van pasiënte lei. Kwaliteit versekerings programme soos “CD Chex®” en “UK Nequas” is beskikbaar, maar baie probleme met verwysing na monster integriteit as gevolg van tydsame vervoer en aflewering kondisies word hiermee geassosieër. Die behoefte het ontstaan vir ‘n laboratorium in Suid Afrika wat direk die omliggende laboratoriums, hospitale en klinieke kan voorsien met gestabiliseerde blood monsters wat gebruik kan word as “IQA”. Die vervoer en aflewerings kondisies van hierdie monsters sal aansienlik verbeter as gevolg van die korter aflewerings afstand wat direk die beperkte temperatuur wisseling beinvloed. Doel van studie: Die doelwit van hoofstuk een is om vir die leser ‘n inleiding te gee tot terminologie en konsepte van immunologie en die immune sisteem. Hoofstuk twee beskyf die impak wat gestabiliseerde heelbloed het op die kliniese immunologie met betrekking tot kwaliteit beheer en kwaliteit versekering. Die doelwit van hierdie studie is om heelbloed te stabiliseer sodat die rakleeftyd meer as 30 dae is en sodoende as verwysings-materiaal kontroles vir Suid Afrikaanse immunofenotipering kan dien. Dit is ‘n verdere doelwit om hierdie tuis-gestabiliseerde kontrole monsters te gebruik as “IQA” verwysings materiaal in verarmende Afrika lande. Die doelwit van hoofstuk vier is om limfosiete te stimuleer om verskeie aktiverings merkers uit te druk op hul selmembrane en dan te stabiliseer en dié te gebruik as Kwaliteits Kontroles vir die meer gespesialiseerde immunologiese toetse. Studie ontwerp: Hoofstuk drie beskryf ‘n aangepaste en verbeterde metode van heel bloed stabiliseering. Stabiliteit word ondersoek in ‘n verskyndenheid konsentrasies van ‘n primêre stabiliseerings agent (chromium chloried heksahidraat) en inkubasie periodes met paraformaldehied as tweede stabiliseerings agent word deeglik gedokumenteer. Bloedmonsters van gesonde indiwidië (n=10) was gestabiliseer en gemonitor vir roetine MIV membraanoppervlak antigene oor ‘n periode van 40 dae. Hierdie monsters (n=10) was gelees en geanaliseer op ‘n BD FACSCalibur™ en vergelyk met ‘n BD FACSCount™ vloeisitometer instrument. Drie gestabiliseerde heelbloed monsters (n=3) was gemonitor vir ‘n periode vir so lank moontlik die fenotipiese selmembraan molekules identifiseerbaar was en die kwantiteit bepaalbaar was. Hierdie drie monsters was gemeet op beide instrumente. As ‘n addisionele doelwit, was hierdie drie gestabiliseerde monsters ondersoek om as moontlike kalibrasie materiaal (verteenwoordig ‘n normale bloedmonster) te dien vir die BD FACSCount™ instrument in die oggende voor pasiënt monsters gelees kan word. In hoofstuk vier was limfosiete geϊsoleer en geaktiveer met ‘n verskyndenheid stimulante om optimale aktiveerings-antigene uit te druk op T helper selmembrane (byvoorbeeld CD25, CD69, HLA-DR en CD40 Ligand). Hierdie geaktiveerde monsters was geanaliseer op die BD FACSCalibur™ en daarna gestabiliseer. Na stabilisasie van die geaktiveerde limfosiet monsters was dit gemonitor oor ‘n tydperk so lank moontlik data plotte leesbaar en selpopulasies identifiseerbaar was. Hierdie monsters kan dien as ‘n moontlike “IQA” toets stel vir ‘n meer gespesialiseerde immunologiese aktiveerings kontrole doeleindes. Resultate: In hoofstuk drie; tien individiële gestabiliseerde heelbloed monsters het gedui op geen-beduidende P waardes (P > 0.05) vir CD3, CD4 en CD8 persentasies en absolute waardes; gemeet vanaf DAG 3 vergelykbaar tot-en-met DAG 40. Met korrelasie statistiek en vergelyking van die BD FACSCalibur™ met die FACSCount™ instrumente, is die volgende opgemerk; R2 = 0.9848 vir die CD4 absolute waardes en ‘n R2 = 0.9636 vir die CD8 absolute waardes. Drie gestabiliseerde monsters (n=3) was gemonitor vir MIV roetine fenotipeering tot en met DAG 84. Die selpopulasies was duidelik identifiseerbaar en die kwantitatief meetbaar op albei instrumente (BD FACSCalibur™ en BD FACSCount™). Hoofstuk vier: geaktiveerde T helper lymphosiete het 25 – 35% membraan CD40 Ligand uitgedruk op hul selmembrane. Die stimulant van keuse was ionomysien teen ‘n optimale konsentrasie van 4μM. Die optimale inkubasie tydperk was vier ure by 37°C in 5% CO2 kondisie. Ses uur inkubasie in 4μM ionomysien by 37°C in ‘n 5% CO2 omgewing was optimal vir die CD69 selmembraan uitdrukking en het 84.21% opgelewer. Vir CD25 selmembraan uitdrukking was die selle vir ses ure met phietoheamagglutinin (PHA) gestimuleer by 37°C in 5% CO2 kondisie en het 43% CD25 selmembraan uitdrukking opgelewer. HLA-DR selmembraan uitdrukking: selle was vir ses ure saam met PHA by 37°C in 5% CO2 kondisie inkubeer en het 43.32% opgelewer. CD40 Ligand aktivering/gestabiliseerde limfosiete het tot en met dag 23 stabiliteit getoon. Die ligand was duidelik identifiseerbaar en kwantifiseerbaar. Geaktiveerde lymphosiete wat CD69, CD25 en HLA-DR selmembraan merkers uitdruk het na die stabiliseerings proses stabiliteit getoon tot-en-met dag 16. Gevolgtrekking: Die doel van hierdie studie was om verwysingskontroles voor te berei sodat dit vars heelbloed naboots met uitkenbare eienskappe vir kliniese situasies. ‘n Toets kontrolestel met verwysings materiaal vir drie vlakke (byvoorbeeld ‘n lae, medium en hoë kontrole) absolute selwaardes en persentasies kan voorberei word vir roetine immunologiese fenotiperings merkers (CD3/CD4/CD8/CD45). Meer gespesialiseerde kontrolestelle vir meer spesifieke doeleindes kan opgemaak word wat ‘n verskydenheid van limfosiet aktiveringsmerkers bevat met byvoorbeeld ‘n “nul”, lae en hoë verwysings kontrole daarin. Hierdie heelbloed kan dien as “aktiveerde interne kwaliteits verwysings materiaal” en kan gebruik word om nuut aangestelde laboratorium werkers en nuut gekwalifiseerde studente op te lei. Hierdie verwysings materiaal / kontroles kan aangewend word vir bevoegdheids doeleindes (byvoorbeeld vir SANAS akkreditasie doeleindes), vir metode ontwikkeling, vir sagteware toetsing, vir paneel opstelling en instrument verstellings doeleindes. Die kontroles moet ‘n verskydenheid eienskappe bevat om effektief te wees. Byvoorbeeld, stabiliteit tydens storing, gewenslik meer as ‘n paar weke, herhaalbaar en maklik handteerbaar. Hierdie kontroles sal inligting voorsien op ‘n daaglikse basis tydens wisseling van tegnieke of instrumentasie wat akuraatheid beinvloed en op die ou-end direk pasiënt versorging bevoordeel.

Small and medium-sized enterprises (SMEs) in Malaysia, particularly In the information and communications technology (lCT) sector, are faced with an increasingly volatile environment. The Malaysian business scene has opened up their markets to the world where smaller businesses find themselves competing with newly launched multinational subsidiary and subdivision companies, along with the large local firms. The Malaysian Government has launched several campaigns and support for smaller local businesses to be more competitive and to continuously compete at par with these larger companies. This research project supports the Malaysian Government's objective of instilling a more structured approach towards a more competitive SME by focusing on the management of competitive information related to these companies. In recognising the rising need for competitive support, management and executives are increasingly relying on a concept called Competitive Intelligence (Cl), a systematic and ethical process for gathering, analysing, and managing information that can affect a company's plans, decisions, and operation. In managing competitive information, several companies have emerged especially to develop online tools and software that would enhance the Cl process and the value competitive intelligence brings to organisations. The success of these Cl software tools depends, however, on the sophistication of an organisation's understanding of the Cl process and scope of usage. Different companies derive different values from different approaches to competitive intelligence, and therefore require a flexible tool that is very specific to the company's needs. Therefore, this research investigated the structures and contexts of Malaysian Small and Mediumsized Enterprises (SMEs) based on competitive intelligence (Cl) concepts to derive a more customised approach to the use of Cl for SMEs in the ICT sector, as well as in the selection of appropriate Cl software. Mintzberg's approaches to analysing organisational structures and contexts, Bouthillier and Shearer's Intelligence Cycle, Herring's Key Intelligence Topics, and Davis' concept of effectiveness were used in two main stages. The first stage involved identifying the nature and range of SMEs, which exist under Malaysia's Multimedia Super Corridor, a government benchmarking body for local businesses. This gives an account, on the basis of cluster analysis, of a taxonomy of SME categories consisted of ten clusters. The relationships between the categories were also examined in the first stage of the research. The relationships and clusters found in the first part of the research offered the basis for the second part of the research, which constructs the criteria for evaluating online tools and software for competitive intelligence. The evaluation criteria are then used to evaluate eight Cl-ready software packages in finding suitable tools for the different categories of SMEs. Finally, the research concludes with a study of the prospective users' perceptions of effectiveness in SMEs drawn from the identified clusters. This 'multiple constituency' approach to understanding effectiveness evaluates both Davis' concept of effectiveness (usefulness), as well as the differential evaluations of perceived effectiveness. The research findings provide evidence of a range of SME structures in a variety of contexts. Levels of importance placed on different levels in the Cl process are identified, as well as aspects that need support, automation and/or augmentation. The software evaluation in the second part of the research provided ten recommendations of suitable software package(s) for each SME cluster. However, an initial review by SME managers of perceived effectiveness mostly did not reveal results that were parallel to the findings from the software evaluation study. All in all, the research confirms that SMEs can be analysed by clusters but further research would be necessary to confirm the effectiveness of using the recommended Cl software over a longer period of time.

A sífilis adquirida e a congênita continuam aumentando e preocupando as autoridades sanitárias do mundo, ao verem que as metas estabelecidas para o seu controle estão longe de serem atingidas. Apesar dos esforços feitos na descoberta de novas ferramentas para o diagnóstico e monitoramento da sífilis, vemos um despreparo muito grande, principalmente entre os laboratoristas, em processar corretamente os reagentes disponíveis e melhor selecionar aqueles que apresentem qualidade para serem usados na rotina do laboratório. O que constatamos neste estudo é um espelho da realidade do diagnóstico da sífilis e mostra a dificuldade que os clínicos enfrentam ao receberem um laudo do laboratório. Trabalhando em colaboração com diferentes serviços e grupos de pesquisa, selecionamos aqueles que trabalhavam com: gestantes atendidas no pré-natal, doadores de sangue, pacientes infectados pelo HIV e pacientes de laboratório clínico e fizemos um estudo crítico da sorologia utilizada em cada serviço. Verificamos discrepância dos resultados obtidos nos testes não-treponêmicos VDRL e RPR, principalmente entre os soros de baixa reatividade, e nos treponêmicos FTA-abs, TPHA e ELISA. Decidimos aplicar o método de Western blotting e analisar o seu comportamento em todas as amostras de soros ensaiadas. Para obtenção de resultados reprodutíveis, fizemos a industrialização do método e formatamos um reagente na forma de um kit diagnóstico, WB Tp-IgG, que pudesse ser facilmente utilizado e interpretado. Os resultados obtidos mostraram que o WB Tp-IgG pode ser utilizado como método confirmatório da sífilis, substituindo o FTA-abs, tradicionalmente recomendado para essa finalidade e que pudesse fazer parte de uma proposta de algoritmo para o diagnóstico sorológico da sífilis. / Acquired and congenital syphilis have been increased and worried the worldwide health authorities, mainly because the WHO targets for syphilis control are far from to be held. Much effort had been made for development of new tools to be used in syphilis diagnosis and following up, however we noticed a lack of ability of laboratory workers in the correctly choosing and using the reagents in laboratory routine. In this study what we observed were the reality of syphilis diagnosis and the difficulties that physicians have in how to procedure when they received the results from the laboratory. Working in collaboration with different settings and research groups we choose some of them that attend pregnant women, blood donors, HIV patients and patients from clinical laboratory. With these group individuals we made a critical study of the serology methods used in each one. We verified high level of discordant results between nontreponemal tests VDRL and RPR, mainly in serum samples with low reactivity and between treponemal tests FTA-abs, TPHA and ELISA, in all services. To obtain reproducibility of the results we made the industrialization of the method and set up a reagent as a kit diagnosis, easily to performer. Appling the western blotting method we evaluated the performance of the test in all sera samples assayed. The results showed that the WB Tp-IgG can be useful as confirmatory test for syphilis, replacing FTA-abs, traditionally recommended for this and that could be included in algorithm propose for serological diagnosis of syphilis.

A sífilis adquirida e a congênita continuam aumentando e preocupando as autoridades sanitárias do mundo, ao verem que as metas estabelecidas para o seu controle estão longe de serem atingidas. Apesar dos esforços feitos na descoberta de novas ferramentas para o diagnóstico e monitoramento da sífilis, vemos um despreparo muito grande, principalmente entre os laboratoristas, em processar corretamente os reagentes disponíveis e melhor selecionar aqueles que apresentem qualidade para serem usados na rotina do laboratório. O que constatamos neste estudo é um espelho da realidade do diagnóstico da sífilis e mostra a dificuldade que os clínicos enfrentam ao receberem um laudo do laboratório. Trabalhando em colaboração com diferentes serviços e grupos de pesquisa, selecionamos aqueles que trabalhavam com: gestantes atendidas no pré-natal, doadores de sangue, pacientes infectados pelo HIV e pacientes de laboratório clínico e fizemos um estudo crítico da sorologia utilizada em cada serviço. Verificamos discrepância dos resultados obtidos nos testes não-treponêmicos VDRL e RPR, principalmente entre os soros de baixa reatividade, e nos treponêmicos FTA-abs, TPHA e ELISA. Decidimos aplicar o método de Western blotting e analisar o seu comportamento em todas as amostras de soros ensaiadas. Para obtenção de resultados reprodutíveis, fizemos a industrialização do método e formatamos um reagente na forma de um kit diagnóstico, WB Tp-IgG, que pudesse ser facilmente utilizado e interpretado. Os resultados obtidos mostraram que o WB Tp-IgG pode ser utilizado como método confirmatório da sífilis, substituindo o FTA-abs, tradicionalmente recomendado para essa finalidade e que pudesse fazer parte de uma proposta de algoritmo para o diagnóstico sorológico da sífilis. / Acquired and congenital syphilis have been increased and worried the worldwide health authorities, mainly because the WHO targets for syphilis control are far from to be held. Much effort had been made for development of new tools to be used in syphilis diagnosis and following up, however we noticed a lack of ability of laboratory workers in the correctly choosing and using the reagents in laboratory routine. In this study what we observed were the reality of syphilis diagnosis and the difficulties that physicians have in how to procedure when they received the results from the laboratory. Working in collaboration with different settings and research groups we choose some of them that attend pregnant women, blood donors, HIV patients and patients from clinical laboratory. With these group individuals we made a critical study of the serology methods used in each one. We verified high level of discordant results between nontreponemal tests VDRL and RPR, mainly in serum samples with low reactivity and between treponemal tests FTA-abs, TPHA and ELISA, in all services. To obtain reproducibility of the results we made the industrialization of the method and set up a reagent as a kit diagnosis, easily to performer. Appling the western blotting method we evaluated the performance of the test in all sera samples assayed. The results showed that the WB Tp-IgG can be useful as confirmatory test for syphilis, replacing FTA-abs, traditionally recommended for this and that could be included in algorithm propose for serological diagnosis of syphilis.

Robotics is an interdisciplinary subject and combines mechanical, computer and electrical engineering components together to solve different kinds of problems. In order to build robotic systems, these disciplines should be integrated. Therefore, mobile robots can be used as a tool in education for teaching engineering concepts. They can be employed to be used in undergraduate, graduate and doctorate research. Hands on experience on a mobile robot increase motivation of the students on the topic and give them precious practical knowledge. It also delivers students new skills like teamwork, problem solving, creativity, by executing robotic exercises. To be able to fulfill these outcomes, universities and research centers need mobile robot platforms that are modular, easy to build, cheap and flexible. However it should be also powerful and capable of being used in different research studies and hence be customizable depending on the requirements of these topics. This thesis aims at building an indoor mobile robot that can be used as a platform for developing algorithms involving various sensors incorporated onto a mobile platform. More precisely, it can be used as a base for indoor navigation and localization algorithms, as well as it can be used as platform for developing algorithms for larger autonomous mobile robots. The thesis work involves the design and manufacturing of a mobile robot platform that can potentially facilitate mobile robotics research that involves use of various hardware to develop and test different perception and navigation algorithms.

This study proposes an industrialization model, Software Factory Automation, for establishing software product lines. Major contributions of this thesis are the conceptualization of Domain Specific Kits (DSKs) and a domain design model for software product lines based on DSKs. The concept of DSK has been inspired by the way other industries have been successfully realizing factory automation for decades. DSKs, as fundamental building blocks, have been deeply elaborated with their characteristic properties and with several examples. The constructed domain design model has two major activities: first, building the product line reference architecture using DSK abstraction / and second, constructing reusable asset model again based on DSK concept. Both activities depend on outputs of feature-oriented analysis of product line domain. The outcome of these coupled modeling activities is the reference architecture and asset model of the product line. The approach has been validated by constructing software product lines for two product families. The reusability of DSKs and software assets has also been discussed with examples. Finally, the constructed model has been evaluated in terms of quality improvements, and it has been compared with other software product line engineering approaches.