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The Global ETD Search service is a free service for researchers
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Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Showing 21 to 22 of 22 (0.175 seconds)Spelling suggestions: "subject:"kvantifiering"" "subject:"kvantifierings""
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Detection and Quantification of PCB insoil using GC/MS : - method development and education for usersSaba, Elias January 2013 (has links)
The aim of this paper is to document the development of a method for detectionand quantification of Polychlorinated biphenyls (PCB) in soil using GasChromatography (GC) connected to a Quadrupole Mass Spectrometer (MS) via aninternal standard (CB189). The method developed is performed in conjunction withthe information provided by the Swedish environmental agency (Svenskanaturvårdsverket, SNV) in regards to PCB limits for sensitive land usage. The steps ofthe method and maintenance of the GC/MS are used to create a user manual andan attempt at transformative learning is done in an effort to teach the staff atLjungaLab AB so that at the very least, independent analysis can be run and at best,new methods and application are independently developed for the GC/MS. Anevaluation of the teaching efforts is also done to assess what grade of learning isachieved. / Syftet med denna uppsats är att dokumentera utvecklingen av en metod fördetektion och kvantifiering av polyklorerade bifenyler (PCB) i jord med hjälp avgaskromatografi (GC) ansluten till en masspektrometer (MS) och med användningav en intern standard (CB189). Metoden som utvecklats skapades med hjälp avuppgifter från det svenska naturvårdsverket (SNV) angående PCB-gränser för känsligmarkanvändning. Därefter skapades en användarmanual som beskriver stegen imetoden och även underhåll av GC/MS. Personalen på LjungaLab AB undervisadesi hur man använder och underhåller instrumenten för att, åtminstone, kunna köraoberoende analyser, och i bästa fall, utveckla nya metoder och tillämpningarsjälvständigt. Det sistnämda är ett försök till transformativ lärande. En utvärdering avlärarinsatser sker också för att bedöma vilken grad av lärande som uppnås.
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Quantification of Pharmaceuticals at the sub-cellular level using the NanoSIMSDost, Maryam January 2024 (has links)
Mass spectroscopy imaging (MSI) has become a vital tool in modern research due to its ability to visualize the spatial distribution of molecules within tissue samples. The collaboration between researchers at AZ, the University of Gothenburg, and Chalmers University of Technology using the NanoSIMS instrument and MSI-SIMS technology has opened up new avenues of exploration in pharmaceutical development, particularly in examining drugs and metabolites at sub-cellular levels. This groundbreaking research has the potential to significantly improve the efficacy and safety of future pharmaceutical products. NanoSIMS possesses a unique imaging and processing technique that enables high-resolution imaging of cellular structures and subcellular compartments. This powerful tool allows for the visualization and measurement of elements and isotopes at the subcellular level. The technique involves bombarding a sample with a focused primary ion beam, which causes the emission of secondary ions. These secondary ions are then analyzed to determine the elemental and isotopic composition of the sample. NanoSIMS is particularly useful for analyzing biomolecules since traditional Mass spectrometry methods cannot provide information about how molecules behave at the cellular level. Given that many of the drugs used today have intra-cellular targets, hence understanding the drug's cellular pathways is extremely important, especially in cases where the risk for organ toxicity is high due to the high dosage of the drugs. Our data from the image analysis indicated the presence of amiodarone inside the lysosomes; however, the lack of enrichment from the 13C portion of the dual-labeled molecule made it difficult to reach a variation below the LOD. Since our LOD is relatively high when working with 13C12C, we focused on the fact that accuracy, precision, and sensitivity would be the most crucial factors in our study. After adjusting these parameters, we obtained an image that made the measurement possible. This project aims to utilize a dual-labeled drug (13C and 127I) to bridge the absolute quantification ability of the 13C labeling scheme to the more sensitive labeling scheme. The focus of this study lies therefore on optimization and the relationship between Spatial resolution, Sensitivity, Mass Resolution, Accuracy, and Precision. This technique is extremely promising, but the limit of detection is relatively high mainly due to the high percentage of carbon in the sample. Despite this fact, we were able to present some valuable data. Our analysis showed that the sensitivity of the 127I is much better than 13C, however, we produced an image where the ratio between the labels was above the detection limit. Using this data, a Relative sensitivity factor (RSF) value was measured, and the concentration of the drug could be estimated by applying the quantification equation.
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