The low-voltage dc-dc converter (LDC) in electrical vehicles (EVs) is to convert high dc voltage (270V~430V) from traction battery to low dc voltage (12.5V~15.5V) for the vehicle auxiliary systems. Galvanic isolation is required in the LDC due to safety considerations. Three challenges exist in the LDC design: (1) wide regulation range; (2) high output current; (3) thermal management. The single stage solutions, such as phase-shift full-bridge converter and LLC resonant converter, have been widely studied in the past. A matrix transformer is widely adopted in single-stage LDC design to deal with the large current. At last, the low-profile design allows large footprint area for high power density and ease of cooling design. However, the trade-off between wide regulation range and efficiency exists in single-stage LDC design. Recently, a two-stage solution is proposed to achieve high efficiency and wide regulation range at the same time. The fixed turn ratio LLC stage serves as a dc transformer (DCX) to meet the galvanic isolation requirements and PWM dc-dc stage regulates the output voltages. In this thesis, a variable ratio matrix transformer-based LLC converter is proposed for two-stage LDC efficiency improvement. The transformer secondary copper losses are reduced by taking advantage of the adaptive number of element transformers. In addition, the PWM dc-dc stage achieves better efficiency with variable intermediate bus voltage. The operation principle and design considerations are studied in this thesis. The proposed 1600W two-stage LDC prototype achieves 96.82% full load efficiency under 400V input condition which is 1.2% efficiency higher than the fixed ratio LLC based two-stage design. Last but not least, the prototype shows a comparable efficiency to the fixed ratio LLC based two-stage design even under the low input voltage (270V) condition. / M.S. / The electrical vehicle market is growing rapidly in recent years. However, the driving range is one of the bottlenecks which imperils market growth in the future. Thus, efficient power modules in electric vehicles are desired to extend the driving range. Low voltage dc-dc converter is one of the power modules in electric vehicles which is rated at several kilowatts and converts traction battery voltage for the vehicle auxiliary system, such as air conditioner, headlights, power steering and etc. In this thesis, a variable ratio matrix transformer-based LLC converter is proposed for the two-stage low-voltage dc-dc converter efficiency improvement. Consequently, the driving range of electric vehicles is further extended.

With the fast development of information technology (IT) industry, the demand and market volume for off-line power supplies keeps increasing, especially those for desktop, flat-panel TV, telecommunication, computer server and datacenter. An off-line power supply normally consists of electromagnetic interference (EMI) filter, power factor correction (PFC) circuit and isolated DC/DC converter. Isolated DC/DC converter occupies more than half of the volume in an off-line power supply and takes the most control responsibilities, so isolated DC/DC converter is the key aspect to improve the overall performance and reduce the total cost for off-line power supply. On the other hand, of all the power supplies for industrial applications, those for the data center servers are the most performance driven, energy and cost conscious due to the large electricity consumption. The total power consumption of today's data centers is becoming noticeable. Moreover, with the increase in cloud computing and big data, energy use of data centers is expected to continue rapidly increasing in the near future. It is very challenging to design isolated DC/DC converters for datacenters since they are required to provide low-voltage high-current output and fast transient response. The LLC resonant converters have been widely used as the DC-DC converter in off-line power supplies and datacenters due to its high efficiency and hold-up capability. Using LLC converters can minimize switching losses and reduce electromagnetic interference. Almost all the high-end offline power supplies employs LLC converters as the DC/DC converter. But there are three major challenges in LLC converters. Firstly, the control characteristics of the LLC resonant converters are very complex due to the dynamics of the resonant tank. This dissertation proposes to implement a special LLC control method, state-trajectory control, with a low-cost microcontroller (MCU). And further efforts have been made to integrate all the state-trajectory control function into one MCU for high-frequency LLC converters, including start-up and short-circuit protection, fast transient response, light load efficiency improvement and SR driving. Secondly, the transformer in power supplies for IT industry is very bulky and it is very challenging to design. By pushing switching frequency up to MHz with gallium nitride (GaN) devices, the magnetics can be integrated into printed circuit board (PCB) windings. This dissertation proposes a novel matrix transformer structure and its design methodology. On the other hand, shielding technique can be employed to suppress the CM noise for PCB winding transformer. This dissertation proposes a novel shielding technique, which not only suppresses CM noise, but also improves the efficiency. The proposed transformer design and shielding technique is applied to an 800W 400V/12V LLC converter design. Thirdly, the LLC converters have sinusoidal current shape due to the nature of resonance, which has larger root mean square (RMS) of current, as well as larger conduction loss, compared to pulse width modulation (PWM) converter. This dissertation employs three-phase interleaved LLC converters to reduce the circulating energy by inter-connecting the three phases in certain way, and proposed a novel magnetic structure to integrated three inductors and three transformers into one magnetic core. By pushing switching frequency up to 1MHz, all the magnetics can be implemented with 4-layer PCB winding. Additional 2-layer shielding can be integrated to reduce CM noise. The proposed magnetic structure is applied to a 3kW 400V/12V LLC converter. This dissertation solves the challenges in analysis, digital control, magnetic design and EMI in high-frequency DC/DC converters in off-line power supplies. With the academic contribution in this dissertation, GaN devices can be successfully applied to high-frequency DC/DC converters with MHz switching frequency to achieve high efficiency, high power density, simplified but high-performance digital control and automatic manufacturing. The cost will be reduced and the performance will be improved significantly. / Ph. D.

The wide-bandgap (WBG) devices, like gallium nitride (GaN) and silicon carbide (SiC) devices have proven to be a driving force of the development of the power conversion technology. Thanks to their distinct advantages over silicon (Si) devices including the faster switching speed and lower switching losses, WBG-based power converter can adopt a higher switching frequency and pursue higher power density and higher efficiency. As a trade-off of the advantages, there also exist the high-frequency-oriented challenges in the adoption of the GaN HEMT under research, including narrow safe gate operating area, increased switching overshoot, increased electromagnetic interference (EMI) in the gate loop and the power stages, the lack of the modules of packages for high current application, high gate oscillation under parallel operation. The dissertation is developed to addressed the all the challenges above to fully explore the potential of the GaN HEMTs. Due to the increased EMI emission in the gate loop, a small isolated capacitor in the gate driver power supply is needed to build a high-impedance barrier in the loop to protect the gate driver from interference. A 2 W dual-output gate driver power supply with ultra-low isolation capacitor for 650 V GaN-based half bridge is presented, featuring a PCB-embedded transformer substrate, achieving 85% efficiency, 1.6 pF isolation capacitor with 72 W/in3 power density. The effectiveness of the EMI reduction using the proposed power supply is demonstrated. The design consideration to build a compact 650 V GaN switching cell is presented then to address the challenges in the PCB layout and the thermal management. With the switching cell, a compact 1 kW 400 Vdc three-phase inverter is built and can operate with 500 kHz switching frequency. With the inverter, the high switching frequency effects on the inverter efficiency, volume, EMI emission and filter design are assessed to demonstrate the tradeoff of the adoption of high switching frequency in the motor drive application. In order to reduce the inverter CM EMI emission above 10 MHz, an active gate driver for 650 V GaN HEMT is proposed to control the dv/dt during turn-on and turn-off independently. With the control strategy, the penalty from the switching loss can be reduced. To build a high current power converter, paralleling devices is a normal approach. The dissertation comes up with the switching cell design using paralleled two and four 650 V GaN HEMTs with minimized and symmetric gate and power loop. The commutation between the paralleled HEMTs is analyzed, based on which the effects from the passive components on the gate oscillation are quantified. With the switching cell using paralleled GaN HEMTs, a 10 kW LLC resonant converter with the integrated litz-wire transformer is designed, achieving 97.9 % efficiency and 131 W/in3 power density. The design consideration to build the novel litz-wire transformer operated at 400 kHz switching frequency is also presented. In all, this work focuses on providing effective solutions or guidelines to adopt the 650 V GaN HEMT in the high frequency, high power density, high efficiency power conversion and demonstrates the advance of the GaN HEMTs in the hard-switched and soft-switched power converters. / Ph. D. / Silicon (Si) -based power semiconductor has developed several decades and achieved numerous outstanding performances, contributing a fast development of the power electronics. While the theatrical limit of the silicon semiconductor is almost reached limiting the progress speed to purse the high-efficiency, high-density high-reliability power conversion, the new material, including gallium-nitride (GaN) and silicon-carbide (SiC), based semiconductor, becomes the driven force to retain the development. Compared with Si-based device, GaN and SiC device own a faster switching speed and a lower on-resistance, enabling the adoption of high switching frequency and the possibility to increase the efficiency, power density and dynamic response. The GaN-based semiconductor is explored to be an even promising game changer than SiC device thanks to a higher theoretical ceiling. However, to adopt GaN-based semiconductors and fully utilize its benefits with high switching frequency, there are numerous high-frequency-oriented challenges, including high frequency oscillation at device termination, increased electromagnetic interference (EMI), the lack of the modules of packages for high current application, high frequency oscillation under parallel operation. The dissertation is developed to address the key high-frequency-oriented challenges to adopt GaN-based semiconductors in the power conversion and come up with the novel design strategy and analysis for high-switching-frequency power conversion using GaN devices. To the reduce the increased EMI emission in the gate loop, a novel PCB-embedded transformer structure is proposed to maintain a low isolation capacitor in the gate driver power supply for the GaN phase leg. With the proposed technique, the dual-output gate driver power supply can achieve high efficiency (85%), ultra-low isolation capacitor (1.6 pF) with high power density (72 W/in³ ). To reduce the high frequency oscillation at the GaN device termination, the strategy to layout GaN devices and its gate driver is proposed with corresponding thermal management. A compact structure for three-phase inverter is then presented, operating with a very high switching frequency (500 kHz). Within the inverter, the high switching frequency effects on the inverter performances are assessed to demonstrate the tradeoff and bottle neck to adopt high switching frequency in the motor drive application. In order to reduce the inverter EMI emission at high frequency ( >10 MHz), an active gate driver for GaN device is proposed for the active dv/dt control strategy. To build a high current power converter, the strategy to parallel GaN devices is proposed in the dissertation with the analysis on the commutation between the paralleled GaN devices. A high-frequency high-current litz-wire transformer structure for LLC resonant converter is presented with modeling and optimization. With the technique, a 10 kW LLC resonant converter achieves high efficiency (97.9 %) and high power density (131 W/in³).

As information technology advances, so does the demand for power management of telecom and computing equipment. High efficiency and high power density are still the key technology drivers for power management for these applications. In order to save energy, in 2005, the U.S. Environmental Protection Agency (EPA) announced the first draft of its proposed revision to its ENERGY STAR specification for computers. The draft specification separately addresses efficiency requirements for laptop, desktop, workstation and server computers. The draft specification also proposes a minimum power supply efficiency of 80% for PCs and 75% to 83% for desktop derived servers, depending on loading condition and server type. Furthermore, recently some industry companies came out with a much higher efficiency target for the whole AC/DC front-end converter over a wide load range. Distributed power systems are widely adopted in the telecom and computing applications for the reason of high performance and high reliability. As one of the key building blocks in distributed power systems, DC/DC converters in the front-end converter are also under the pressure of increasing efficiency and power density. Due to the hold-up time requirement, PWM DC/DC converters cannot achieve high efficiency for well known reasons when they are designed for wide input voltage range. As a promising topology for this application, LLC resonant converters can achieve both high efficiency and wide input voltage range capability because of its voltage gain characteristics and small switching loss. However, the efficiency of LLC resonant converter with diode rectifier still cannot meet the recent efficiency target from industry. In order to further improve efficiency of LLC resonant converters, synchronous rectification must be used. The complete solution of synchronous rectification of LLC resonant converters is discussed in this thesis. The driving of the synchronous rectifier can be realized by sensing the voltage Vds of the SR. The turn-on of the SR can be triggered by the body-diode conduction of the SR. With the Vds compensation network, the precise voltage drop on Rds_on can be achieved, thus the SR can be turned off at the right time. Moreover, efficiency optimization at normal operation over wide load range is discussed. It is revealed that power loss at normal operation is solely determined by the magnetizing inductance while the magnetizing inductor is designed according to dead-time td selection. The mathematic equations for the relationship between power loss and dead-time are developed. For the first time, the relationship between power loss and dead-time is used as a tool for efficiency optimization. With this tool, the efficiency optimization of the LLC resonant converter can be made according to efficiency requirement over a wide load range. With the expectation to achieve high efficiency at ultra-light load, the green mode operation of LLC resonant converters is addressed. The rationale of the issue with the conventional control algorithm is revealed and a preliminary solution is proposed. / Master of Science

An ever present trend in the power conversion industry is to get higher performance at a lower cost. In a computer server system, the front-end converter, supplying the load subsystems, is typically a multiple output power supply. The power supply unit is custom designed and its output voltages are fully regulated, so it is not very efficient or cost effective. Most of the load systems in this application are supplied by point-of-load converters (POLs). By leaving the output voltage regulation aspect to POLs, the front-end converter does not need to be a fully regulated, multiple output converter. It can be replaced by a dc-dc transformer (DCX), which is a semi-regulated or unregulated, single output dc-dc converter. A DCX can be made using a modular design to simplify expansion of the system capacity. To realize this concept, the DCX block must have a current sharing feature. The current sharing method for a resonant DCX is discussed in this work. To simplify the system architecture, the current sharing method is based on the droop method, which requires no communication between paralleled units. With this method, the current sharing error is inversely proportional to the droop voltage. In traditional DCX implementations, the droop voltage depends on the resistive voltage drops in the power stage, which is not sufficient to achieve the desired current sharing error. The resonant converter has the inherent characteristic that its conversion gain depends on the load current, so the virtual droop resistance can realized by the resonant tank and the droop voltage can be obtained without incurring conduction loss. An LLC resonant converter is investigated for its droop characteristic. The study shows the required droop voltage is achievable at very high switching frequency. To lower the switching frequency, a notch filter is introduced into the LLC resonant tank to increase the sensitivity of the conversion gain versus the operating frequency. The design of the multi-element resonant tank is discussed. Depending soly on the resonant tank, the droop characteristic is largely varied with the component tolerance in the resonant tank. The current sharing error becomes unacceptable. The active droop control is imposed to make the output regulation characteristic insensitive to the component tolerance. The proposed resonant DCX has simpler circuit structure than the fully regulated resonant converter. Finally simulation and experimental results are presented to verify this concept. / Master of Science

Isolated inverters feature the freedom in voltage step-up/down, electrical safety, and modularity. Among them, pseudo-dc-link inverters have the advantage of high efficiency due to their single-stage structure. Traditionally, pseudo-dc-link inverters are based on pulse-width-modulated converters, which suffer from hard switching, the need for auxiliary components, and/or high current stresses. Meanwhile, the series resonant converter has been prevalent in past decades due to its simplicity and high efficiency. Therefore, it is intriguing to design a single-stage inverter based on a series resonant converter. However, there are limited papers regarding such an inverter topology. To figure out the reason, basic modulation methods proposed or implied in the literature are summarized and evaluated through circuit simulation software. It turns out each basic modulation method has at least one critical drawback in modulation range, hard switching, and/or high current stresses. Given the deficiencies in the basic modulation methods, a hybrid modulation method is proposed here. The proposed method combines variable-frequency modulation in the high-output region and short pulse-density modulation in the low-output region. In this way, all the aforementioned critical drawbacks can be greatly alleviated. The hybrid modulation method is compared to the basic modulation methods based on three design metrics: the rms value of the resonant current, the magnetic flux of the transformer, and the turn-off current. By these design metrics that directly related to power losses, the benefit of the proposed method in terms of efficiency can be explained. Moreover, a power loss model is also established to provide more insights into the inverter's efficiency performance. It helps demonstrate how the selection of resonant tank and other factors affects the power loss distribution. Also, an inverter design procedure is introduced and a prototype is built to verify the proposed modulation method. The results show that the switching losses, especially the turn-on loss, can be well suppressed, and the losses in other passive components are well restrained. This implies the proposed method is suitable for high-frequency applications. Other than efficiency, output waveform quality is also important for an inverter. However, the changing plant model makes the controller design difficult. Therefore, a third-order model established by other researchers has been adopted to identify the pole locations. In addition, a gain-varying method is proposed for the compensator to reduce the gain variance caused by different operating conditions. The experimental results show that without the gain-varying method, the inverter may have issues in slow tracking and/or instability. Finally, in some scenarios, the inverter based on a series resonant converter can be regarded as a module. A multi-modular inverter can be formed by connecting the modules in an input-parallel-output-series configuration. In this case, a technique termed sequential waveform synthesis can be applied. The proposed technique can extend the region of variable-frequency modulation and shorten the region of short pulse-density modulation. This is beneficial to efficiency based on an analysis. With more than a certain amount of modules connected, the short pulse-density modulation can even be waived, which means the multi-modular inverter can be free from turn-on loss. In summary, this dissertation focuses on developing modulation methods for inverters based on the series resonant converter. Soft-switching feature and high efficiency are the two top priorities. The analytic and experimental results are provided based on standalone applications. / Doctor of Philosophy / Inverters are an important part of a modern electric power system, as they convert dc electric power into ac electric power. In some applications, inverters with electrical insulation (isolated inverters) are preferred due to the need for engineering freedom, safety, and other reasons. However, each conventional isolated inverter has some of the following drawbacks: hard-switching in semiconductor devices, high circulating current, poor transformer utilization, and high complexity. These drawbacks limit the efficiency and compactness of an inverter system, making the system less attractive to practical applications. An inverter based on a series resonant converter seems to be a solution because the series resonant converter is known for being simple and highly-efficient. However, there has yet to be a proper modulation method for it. Therefore, the main contribution of this dissertation is to propose a hybrid modulation method. With the proposed method, the inverter can operate with high efficiency. Furthermore, the hard-switching can be well suppressed, which means a high-frequency, compact design is possible. Besides the theory of the proposed method, this dissertation also includes a power loss model, a hardware design procedure, and analytic comparisons with other methods. In addition, a digital approach to control the inverter is proposed. Without it, the output voltage waveform may be highly distorted. Finally, another sequential control strategy is proposed in this dissertation for an integrated system. The integrated system is composed of multiple inverters based on a series resonant converter. With the sequential control strategy, the overall output waveform quality of the integrated system can be improved.

In comparison with PWM converters, resonant converters are gaining increasing popularity for cases in which efficiency and power density are at a premium. However, the lack of an accurate small-signal model has become an impediment to performance optimization. Many modeling attempts have been made to date. Besides the discrete time-domain modeling, most continuous-time modeling approaches are based on fundamental approximation, and are thus unable to provide sufficient accuracy for practical use. An equivalent circuit model was proposed by Yang, which works well for series resonant converters (SRCs) with high Q (quality factor), but which is inadequate for LLC resonant converters. Furthermore, the model is rather complicated, with system orders that are as high as five and seven for the SRC and LLC converter, respectively. The crux of the modeling difficulty is due to the underlying assumption based on the use of a band-pass filter for the resonant tank in conjunction with a low-pass output filter, which is not the case for most practical applications. The matter is further complicated by the presence of a rectifier, which is a nonlinearity that mixes and matches the original modulation frequency. Thus, the modulation signal becomes intractable when using a frequency-domain modeling approach. This dissertation proposes an extended describing function modeling that is based on a Fourier analysis on the continuous-time-domain waveforms. Therefore, all important contributions from harmonics are taken into account. This modeling approach is demonstrated on the frequency-controlled SRC and LLC converters. The modeling is further extended to, with great accuracy, a charge-controlled LLC converter. In the case of frequency control, a simple third-order equivalent circuit model is provided with high accuracy up to half of the switching frequency. The simplified low-frequency model consists of a double pole and a pair of right-half-plane (RHP) zeros. The double pole, when operated at a high switching frequency, manifests the property of a well-known beat frequency between the switching frequency and the resonant frequency. As the switching frequency approaches the resonant frequency of the tank, a new pair of poles is formed, representing the interaction of the resonant tank and the output filter. The pair of RHP zeros, which contributes to additional phase delay, was not recognized in earlier modeling attempts. In the case of charge control, a simple second-order equivalent circuit model is provided. With capacitor voltage feedback, the order of the system is reduced. Consequently, the resonant tank behaves as an equivalent current source and the tank property is characterized by a single pole. The other low-frequency pole represents the output capacitor and the load. However, the capacitor voltage feedback cannot eliminate the high-frequency poles and the RHP zeros. These RHP zeros may be an impediment for high-bandwidth design if not properly treated. Based on the proposed model, these unwanted RHP zeros can be mitigated by either changing the resonant tank design or by proper feedback compensation. The accurate model is essential for a high-performance high-bandwidth LLC converter. / Doctor of Philosophy / For high-frequency power conversion, resonant converters are increasingly popular. However, the lack of an accurate small-signal model has become an impediment to performance optimization. The existing equivalent circuit model and its simplified circuit were based on fundamental approximation, where the resonant tank was deemed a good band-pass filter. These models work well for series resonant converters (SRCs) with high Q (quality factor), but are inadequate for LLC resonant converters. The crux of the modeling difficulty is due to the fact that the operation of this type of resonant converter is based on the use of a band-pass filter in conjunction with a low-pass filter. The matter is further complicated by the presence of a rectifier, which is a nonlinearity that mixes and matches the original modulation frequency. Thus, the modulation signal becomes intractable when using a frequency-domain modeling approach. This dissertation proposes an extended describing function modeling that is based on a Fourier analysis on the continuous-time-domain waveforms. Therefore, all important contributions from harmonics are taken into account. This modeling approach is demonstrated on the frequency-controlled SRC, frequency-controlled LLC converter, and charge-controlled LLC converter, and the resulting models are proven to be accurate at all frequencies. A simple equivalent circuit model is provided that targets the frequency range below the switching frequency. This simple, accurate model is able to predict the small-signal behaviors of the LLC converter with high accuracy at half of the switching frequency. At high modulation frequencies, the resonant converter behaves like a non-minimum phase system, which was neither recognized nor characterized before. This property can be represented by RHP zeros, and these RHP zeros may be an impediment for high-bandwidth design if not properly treated. Based on the proposed model, these unwanted RHP zeros can be mitigated by either changing the resonant tank design or by proper feedback compensation. Accurate modeling is essential for a high-performance high-bandwidth LLC converter.

With the rapid increase of cloud computing and the high demand for digital content, it is estimated that the power consumption of the IT industry will reach 10 % of the total electric power in the USA by 2020. Multi-core processors (CPUs) and graphics processing units (GPUs) are the key elements in fulfilling all of the digital content requirements, but come with a price of more power-hungry processors, driving the power per server rack to 20 KW levels. The need for more efficient power management solutions on the architecture level, down to the converter level, is inevitable. Recently, data centers have replaced the 12V DC server rack distribution with a 48V DC distribution, producing a significant overall system efficiency improvement. However, 48V rack architecture raises significant challenges for the voltage regulator modules (VRMs) required for powering the processor. The 48V VRM in the vicinity of the CPU needs to be designed with very high efficiency, high power density, high light-load efficiency, as well as meet all transient requirements by the CPU and GPU. Transferring the well-developed multi-phase buck converter used in the 12V VRM to the 48V distribution platform is not that simple. The buck converter operating with 48V, stepping down to sub 2V, will be subjected to significant switching related loss, resulting in lower overall system efficiency. These challenges drive the need to look for more efficient architectures for 48V VRM solutions. Two-stage conversions can help solve the design challenges for 48V VRMs. A first-stage unregulated converter is used to step-down the 48V to a specific intermediate bus voltage. This voltage will feed a multi-phase buck converter that powers the CPU. An unregulated LLC converter is used for the first-stage converter, with zero voltage switching (ZVS) operation for the primary side switches, and zero current switching (ZCS) along with ZVS operation, for the secondary side synchronous rectifiers (SRs). The LLC converter can operate at high frequency, in order to reduce the magnetic components size, while achieving high-efficiency. The high-efficiency first-stage, along with the scalability and high bandwidth control of the second-stage, allows this architecture to achieve high-efficiency and power density. This architecture is simpler to adopt by industry, by plugging the unregulated converter before the existing multi-phase buck converters on today's platforms. The first challenge for this architecture is the transformer design of the first-stage LLC converter. It must avoid all of the loss associated with high frequency operations, and still achieve high power density without scarifying efficiency. In this thesis, the integrated matrix transformer structure is optimized by SR integration with windings, interleaved primary side termination, and a better PCB winding arrangement to achieve high-efficiency and power density, and minimize the losses associated with high-frequency operations. The second challenge is the light load efficiency improvement. In this thesis a light load efficiency improvement is proposed by a dynamic change of the intermediate bus voltage, resulting in more than 8 % light load efficiency improvements. The third challenge is the selection of the optimal bus voltage for the two-stage architecture. The impact of different bus voltages was analyzed in order to maximize the overall conversion efficiency. Multiple 48V unregulated converters were designed with maximum efficiency >98 %, and power densities >1000 W/in3, with different output voltages, to select the optimal bus voltage for the two-stage VRM. Although the two-stage VRM is more scalable and simpler to design and adopt by current industry, the efficiency will reduce as full power flows in two cascaded DC/DC converters. Single-stage conversion can achieve higher-efficiency and power-density. In this thesis, a quasi-parallel Sigma converter is proposed for the 48V VRM application. In this structure, the power is shared between two converters, resulting in higher conversion efficiency. With the aid of an optimized integrated magnetic design, a Sigma converter suitable for narrow voltage range applications was designed with 420 W/in3 and a maximum efficiency of 94 %. Later, another Sigma converter suitable for wide voltage range applications was designed with 700W/in3 and a maximum efficiency of 95 %. Both designs can achieve higher efficiency than the two-stage VRM and all other state-of-art solutions. The challenges associated with the Sigma converter, such as startup and closed loop control were addressed, in order to make it a viable solution for the VRM application. The 48V rack architecture requires regulated 12V output converters for various loads. In this thesis, a regulated LLC is used to design a high-efficiency and power-density 48V bus converter. A novel integration method of the inductor and transformer helps the LLC achieve the required regulation capability with minimum losses, resulting in a converter that can provide 1KW of continuous power with efficiency of 97.8 % and 700 W/in3 power density. This dissertation discusses new power architectures with an optimized design for the 48V rack architectures. With the academic contributions in this dissertation, different conversion architectures can be utilized for 48V VRM solutions that solve all of the challenges associated with it, such as scalability, high-efficiency, high density, and high BW control. / Doctor of Philosophy / With the rapid increase of cloud computing and the high demand for digital content, it is estimated that the power consumption of the IT industry will reach 10 % of the total electric power in the USA by 2020. Multi-core processors (CPUs) and graphics processing units (GPUs) are the key elements in fulfilling all of the digital content requirements but come with a price of more power-hungry processors, driving the power per server rack to 20 KW levels. The need for more efficient power management solutions on the architecture level, down to the converter level, is inevitable. The data center manufacturers have recently adopted a more efficient architecture that supplies a 48V DC server rack distribution instead of a 12V DC distribution to the server motherboard. This helped reduce costs and losses, but as a consequence, raised a challenge in the design of the DC/DC voltage regulator modules (VRM) supplied by the 48V, in order to power the CPU and GPU. In this work, different architectures will be explored for the 48V VRM, and the trade-off between them will be evaluated. The main target is to design the VRM with very high-efficiency and high-power density to reduce the cost and size of the CPU/GPU motherboards. First, a two-stage power conversion structure will be used. The benefit of this structure is that it relies on existing technology using the 12V VRM for powering the CPU. The only modification required is the addition of another converter to step the 48V to the 12V level. This architecture can be easily adopted by industry, with only small modifications required on the system design level. Secondly, a single-stage power conversion structure is proposed that achieves higher efficiency and power density compared to the two-stage approach; however, the structure is very challenging to design and to meet all requirements by the CPU/GPU applications. All of these challenges will be addressed and solved in this work. The proposed architectures will be designed using an optimized magnetic structure. These structures achieve very high efficiency and power density in their designed architectures, compared to state-of-art solutions. In addition, they can be easily manufactured using automated manufacturing processes.

In eukaryotischen Zellen sind die Lipidspezies häufig asymmetrisch zwischen den Hälften der Plasmamembran verteilt. Insbesondere Phosphatidylserin (PS) weist oft eine ausgeprägte transversale Asymmetrie auf, da es fast ausschliesslich auf die innere Hälfte der Plasmamembran beschränkt ist. In den letzten Jahren wurden mehrere Proteine diskutiert, die Lipide zwischen den Membranhälften transportieren und möglicherweise die transversale Lipidasymmetrie sowie damit verbundene Zelleigenschaften beeinflussen. Im Mittelpunkt der vorliegenden Promotion steht der Auswärtstransport fluoreszierender (C6-NBD-) Lipid-Analoga und endogener Lipide durch das Multidrug Resistance 1 P-Glycoprotein (MDR1 Pgp), das der ATP Binding Cassette (ABC) Transporter Superfamilie angehört. Interessanter Weise wird für MDR1 Pgp eine ungewöhnlich breite Substratspezifität angenommen. Das anionische Lipid PS war hier von besonderem Interesse, obgleich es in vorhergehenden Arbeiten nicht als MDR1 Pgp Substrat betrachtet wurde. Der Auswärtstransport von Phosphatidylcholin-, Phosphatidylethanolamin-, Glucosylceramid- und Sphingomyelin-Analoga durch MDR1 Pgp konnte in einer humanen Magenkarzinomlinie (EPG85-257), die MDR1 überexprimiert, mittels Fluoreszenzspektroskopie bestätigt werden. Zudem legt die verringerte Akkumulation von Diacylglycerol- und Ceramid-Analoga den Transport dieser Lipidspezies durch MDR1 Pgp nahe. Im Anschluß an die intrazelluläre Markierung mit C6-NBD-PS mittels eines neuen Verfahrens konnte der signifikant erhöhte Auswärtstransport dieses Analogons in MDR1 überexprimierenden Zellen durch Verwendung spezifischer Inhibitoren MDR1 Pgp zugeschrieben werden. In flusscytometrischen Versuchen war die Exponierung von endogenem PS auf der äusseren Membranhälfte von MDR1 überexprimierenden Zellen signifikant höher als in Kontrollzellen. Verringerung der PS-Exponierung durch einen Inhibitor von MDR1 Pgp deutet auf den Transport von endogenem PS durch MDR1 Pgp hin. Zusätzlich wurde hier der Transport von C6-NBD-PS in vier weiteren Zellinien mit verschiedener Spezies- und Gewebezugehörigkeit charakterisiert, die unterschiedliche Mengen an MDR1 Pgp synthetisieren. Wie Experimente in einer BCRP überexprimierenden EPG85-257-Sublinie nahelegen, ist ausser MDR1 Pgp möglicherweise ebenfalls der ABC Halb-Transporter Breast Cancer Resistance Protein (BCRP) am Transport von C6-NBD-PS und an der verstärkten Exponierung von endogenem PS beteiligt. / In eukaryotic cells, the lipid species are frequently distributed asymmetrically between the plasma membrane leaflets. Phosphatidylserine (PS), in particular, often exhibits a distinct transverse asymmetry, being restricted almost exclusively to the inner leaflet. In the past years, several proteins were suggested to transport lipids between the leaflets of a membrane, and to potentially influence transverse lipid asymmetry and related cell properties. This thesis focuses on outward transport of fluorescent (C6-NBD-) lipid analogs and endogenous lipids by the Multidrug Resistance 1 P-Glycoprotein (MDR1 Pgp), a member of the ATP binding cassette (ABC) transporter superfamily. Interestingly, MDR1 Pgp has been suggested to exhibit an unusually broad substrate specificity. Here, the anionic PS was of particular concern, although previously reported not to be an MDR1 Pgp substrate. In a human gastric carcinoma cell line (EPG85-257) overexpressing MDR1, outward transport of phosphatidylcholine, phosphatidylethanolamine, glucosylceramide and sphingomyelin analogs via MDR1 Pgp was confirmed using fluorescence spectroscopy. In addition, decreased accumulation of analogs of diacylglycerol and ceramide suggest MDR1 Pgp mediated transport of these lipid species. Upon intracellular labelling with C6-NBD-PS using a novel approach, significantly increased outward transport of this analog in MDR1 overexpressing cells could be attributed to MDR1 Pgp by employing specific inhibitors. In a flow cytometry setup, the exposure of endogenous PS on the outer plasma membrane leaflet was significantly elevated in MDR1 overexpressing cells compared to controls. Reduction of PS exposure by an MDR1 Pgp inhibitor suggests transport of endogenous PS by MDR1 Pgp. Transport of C6-NBD-PS was furthermore characterized here in four additional cell lines of different species and tissue origin with varying synthesis levels of MDR1 Pgp. Besides MDR1 Pgp, the ABC half-size transporter Breast Cancer Resistance Protein (BCRP) is possibly also involved in transport of C6-NBD-PS and in increased exposure of endogenous PS, as found in a BCRP overexpressing EPG85-257 subline.

Compostos α-aminocarbonilícos como ácido 5-aminolevulínico (ALA) e aminoacetona (AA) apresentam um grande potencial pró-oxidante, pois sofrem reações de enolização e subseqüente oxidação aeróbica, com a formação de espécies radicalares de oxigênio, íons NH4+ e α-oxoaldeídos potencialmente citotóxicos. A α-aminocetona 1,4-diamino-2-butanona (DAB), um análogo da putrescina, é um agente microbicida de vários parasitas incluindo Trypanosoma cruzi. Acredita-se que o mecanismo de morte desencadeado por DAB nos parasitas seja por meio da inibição competitiva da ornitina descarboxilase (ODC), importante enzima do metabolismo de poliaminas, muito embora tenha sido observado de igual forma danos oxidativos nestes parasitas quando tratados com DAB. O objetivo deste trabalho é esclarecer o mecanismo de oxidação química de DAB e sua ação pró-oxidante à cultura de células de mamíferos (LLC-MK2 e RKO), assim como sua atividade microbicida contra tripomastigotas de Trypanosoma cruzi. Demonstramos aqui que DAB, quimicamente similar ao ALA e AA, sofre reação de oxidação catalisada por íons fosfato, e por íons de metais de transição como Fe(II) e Cu(II), resultando na formação de radicais de oxigênio, H2O2, NH4+, 2-oxo-4-aminobutanal como produto principal da oxidação de DAB e de compostos ciclicos de caracter pirrólico. Danos oxidativos observados em ferritina, apotransferrina e liposomos de cardiolipina e fosfatidilcolina (20:80) contribuem para a nossa hipótese de ação pró-oxidante de DAB. O tratamento de células de mamíferos das linhagens LLC-MK2 (IC50 1,5 mM, tratamento de 24 h) e RKO (IC50 0,3 mM, tratamento de 24 h) com DAB levou à alteração do balanço redox celular, à ativação de resposta antioxidante e ao desencadeamento de morte celular via apoptose e parada de ciclo celular. Em culturas de tripomastigotas de T. cruzi o tratamento com DAB culminou na redução da motilitidade e viabilidade destes parasitas (IC50 0,2 mM, tratamento de 4 h), assim como depleção do conteúdo tiólico acompanhado pelo aumento da atividade de TcSOD. Além do mais, DAB mostrou-se eficiente em limitar a invasão de tripomastigotas às células hospedeiras (LLC-MK2) e reduzir a proliferação de amastigotas intracelulares, contudo fortemente relacionada à necrose das células hospedeiras infectadas, uma vez que são alvos mais susceptíveis de ação oxidativa. Estes resultados suportam nossa hipótese que DAB exerce ação pró-oxidante e contribui deste modo com o mecanismo já descrito de morte celular associada à inibição da biossíntese de poliaminas em vários microorganismos. / α-Aminocarbonyl componds such as 5-aminolevunilic acid (ALA) and aminoacetone (AA) have been shown to exhibit pro-oxidant properties. These compounds undergo phosphate-catalyzed enolization in physiological pH and subsequent aerobic oxidation, yielding reactive oxygen species, NH4+ ions and an α-oxoaldehyde highly cytotoxic. The &#945-aminoketone 1,4-diamino-2-butanone (DAB) is a putrescine analogue and a microbicidal agent to various parasites including Trypanosoma cruzi. The mechanism of DAB toxicity to these parasites is attributed to DAB competitive inhibition of ornithine decarboxylase (ODC), a key enzyme on polyamine biosynthesis, although it has also been shown DAB isto implicated in oxidative damage to these parasites. Our aim is to clarify the mechanism of DAB aerobic oxidation and of its putative pro-oxidant activity to mammalian cell cultures (LLC-MK2 and RKO cell linages) and to Trypanosoma cruzi trypomastigotes. Here we show that, similar to ALA and AA, DAB undergoes aerobic oxidation in presence of phosphate ions and of transition metal ions such as Fe(II) and Cu(II), yielding oxygen radicals, H2O2, NH4+ and 2-oxo-4-aminobutanal accompanied by its condensation cyclic products displaying pyrrolic characteristics. Oxidative alterations to ferritin, apotransferrin and liposomes of cardiolipin and phosphatidylcholine (20:80) were observed under DAB treatment strongly supporting our hypothesis of DAB pro-oxidative activity. DAB treatment of mammalian cultured cells LLC-MK2 (IC50 1.5 mM, 24 h incubation) and RKO (IC50 0.3 mM, 24 h incubation) resulted in redox imbalance, induction of antioxidant response, activation of apoptosis pathway and cell cycle arrest. DAB is shown here to trigger Trypanosoma cruzi trypomastigotes decreased parasite motility and viability (IC50 0.2 mM, 4 h incubation), as well as redox thiol imbalance parallel to increase TcSOD activity. In addition, DAB efficiently hampered host cell (LLC-MK2) invasion by trypomastigotes. In addition, intracellular amastigotes showed to be susceptible to DAB toxicity, although strongly related to necrosis of infected host cells, which are more vulnerable to oxidative stress. Altogether, these data support our hypothesis that oxidative stress contributes to DAB cytotoxicity.