Victoria blight, caused by fungus Cochliobolus victoriae, is a disease originally described on oats and recapitulated on Arabidopsis. Victoria blight is used as a model plant disease that conforms to an inverse gene-for-gene interaction. C. victoriae virulence is dependent upon its production of victorin, a host-specific toxin that induces programmed cell death in sensitive plants. In oats, victorin sensitivity and disease susceptibility is conferred by the Vb gene, which is genetically inseparable from the Pc-2 crown rust resistance gene. In Arabidopsis, victorin sensitivity and disease susceptibility is conferred by the LOCUS ORCHESTRATING VICTORIN EFFECTS 1 (LOV1) gene which encodes a NB-LRR protein, a type of protein commonly associated with disease resistance. LOV1-mediated cell death occurs when victorin binds Thioredoxin-h5 (TRX-h5) and LOV1 appears to "guards" TRX-h5. Together, these results suggest C. victoriae causes disease by inducing a resistance response. The work presented here aimed to determine if the response mediated by LOV1 is functionally related to a resistance response. We genetically characterized the response mediated by LOV1 with virus-induced gene silencing. We determined SUPPRESSOR OF THE G2 ALLELE OF SKP1 (SGT1), a gene required for the function of many resistance genes, is required for victorin sensitivity and involved in LOV1 protein accumulation. We screened a normalized library and identified six genes that suppressed victorin-mediated cell death and cell death induced by expression of the RESISTANCE TO PERONOSPORA PARASITICA PROTEIN 8 (RPP8) resistance gene: a mitochondrial phosphate transporter, glycolate oxidase, glutamine synthetase, glyceraldehyde 3-phosphate dehydrogenase and the P- and T-protein of the glycine decarboxylase complex. Silencing the latter four also inhibited cell death induced by the expression of an autoactive form of the resistance gene PTO, and reduced PTO-mediated resistance to Pseudomonas syringae pv. tabaci. These results provide evidence that victorin-mediated cell death is functionally similar to a resistance response, further supporting the hypothesis that a resistance response is exploited by C. victoriae for pathogenesis in Victoria blight. Resistance function of LOV1 was evaluated by observing Pseudomonas syringae pv. tomato virulence upon LOV1 activation. The LOV1 response pathway in Arabidopsis was adapted to activate upon infection with Pseudomonas syringae pv. tomato expressing the type III-dependent effector protein AvrRpt2, a well-characterized protease. We developed a construct to express a beta-glucuronidase (GUS) and TRX-h5 fusion protein separated by an AvrRpt2 proteolytic cleavage site, in which GUS sterically inhibits TRX-h5 function in LOV1-mediated cell death. The fusion is cleaved upon infection by P. syringae pv. tomato expressing avrRpt2, thereby leading to TRX-h5-mediated activation of LOV1 in the presence of victorin. However, when this strain was inoculated with victorin into transgenic LOV1 trx-h5 plants expressing the GUS/TRX-h5 fusion protein, no decrease in pathogen virulence was observed. Technical shortcomings likely prevented observable LOV1 resistance function. ��� / Graduation date: 2013 / Access restricted to the OSU Community at author's request from Oct. 9, 2012 - Oct. 9, 2013

Syftet med uppsatsen är att utföra en retoriskanalys på de argument som förs bland medlemmarna på Facebookgrupperna ”Vägra betala Victorias bröllop!” respektive ”Våga betala Victoria och Daniels bröllop”. Grupperna är två intressanta debattforum att studera i syfte att ta reda på vad för typ av dialog som förs liksom gruppmedlemmarnas åsikter kring det kungliga bröllopet såväl som monarkin. Således kommer uppsatsen att fokusera på den medierande offentlighet som skapas i samspel med interaktionen som äger rum på respektive grupp.   Uppsatsens utgår från tre frågeställningar: Förs en kommunikativ dialog i frågan kring för respektive emot det kungliga bröllopet på Facebook? Hur är dialogerna om bröllopet uppbyggda? Vad har medlemmarna för åsikter kring det kungliga bröllopet så väl som monarkin? Genom att fokusera på en för- respektive emotgrupps åsikter på Facebook tangerar uppsatsen olika forskningsfält såsom: forskning om det kungliga bröllopet 2010, det Internetbaserade forumet Facebook men även fältet för retorik och medierad offentlighet. Den kvalitativa forskningsmetoden har använts vid studium av grupperna.   Genom att utföra en kvalitativ studie av respektive Facebookgrupp kan man fastställa att det förs en kommunikativ dialog inom grupperna såtillvida att den är ömsesidig och öppenhjärtig mellan de likasinnade medlemmarna. För emotgruppen ”Vägra betala Victorias bröllop!” saknar det kungliga bröllopet och monarkin nationell betydelse. Mina studier av de många inläggen i forumet för gruppen vittnar om att medlemmarna är emot bröllopet och monarkin i sin helhet. Förgruppen ”Våga betala Victoria och Daniels bröllop!” anser däremot att monarkin har en representativ betydelse och att monarkin symboliserar Sverige som nation. Mina studier visar på att medlemmarna är för bröllopet och bevarandet av monarkin, detta blir synligt genom medlemmarnas positiva tillrop och lyckohälsningar till Kronprinsessan i gruppens forum.   Jag har haft hjälp av James Slevins teori kring medierad offentlighet skapad av Internet; en teori som bygger vidare på John B. Thompsons tankar och idéer kring den medierade offentlighet som var gällande i en tid präglad masskommunikation. Till sist kan jag, med stöd i de analyser jag utfört, konstatera att Facebook snarare främjar en Internetbaserad medierad offentlighet grundad i Slevins utveckling av Thompsons teori än en deliberativ medierad offentlighet.   Det har aldrig tidigare publicerats en uppsats eller vetenskaplig publikation som förenar mina studieobjekt och val av teorier. Av tidsbegränsade själ kommer uppsatsen inte att se till historievetenskaplig forskning i analysen av synen på monarkin och kungafamiljen. Heller inte till tidigare forskning om Facebook.   Nyckelord:      Argumentationsanalys, Det kungliga bröllopet, Ethos, Facebook, Facebookgrupper, Internet, Logos, Medierad offentlighet, Pathos, Retorikanalys, ”Våga betala Victoria och Daniels bröllop!”, ”Vägra betala Victorias bröllop!”.

My doctoral thesis challenges the traditional paradigm of phosphorus (P) limitation of phytoplankton communities in freshwaters by suggesting colimitation of P, nitrogen (N), and iron (Fe) in Great Lakes. Oceanographers have recognized Fe, N and P colimitation, and biomass response to Fe is documented in freshwater lakes. I studied African and North American Great Lakes that are similar to large inland oceans. I discovered that Fe is a key nutrient that is often limiting in the offshore, and may explain the dominance of cyanobacteria in nutrient enriched lakes. I also discovered that the nearshore and offshore areas of these large lakes are very different, particularly when invasive dreissenid mussels are impacting the nearshore, as seen in the eastern basin of Lake Erie. As a result of the dreissenids, chlorophyll a (chla) concentrations are significantly lower in the nearshore of Lake Erie, but higher in the nearshore in the three African Great Lakes, as well as pre-dreissenid Lake Erie. The objective of my thesis was to determine the limiting nutrient(s) to the phytoplankton of the Great Lakes Erie, Malawi, Tanganyika, and Victoria in both the nearshore and offshore by measuring the physiological status of the phytoplankton. I also examined how dreissenids affect the distribution of seston and nutrient concentrations between the nearshore and offshore of the eastern basin of Lake Erie. My study design included temporal and spatial surveys in the nearshore and offshore of the four lakes, in which I used a variety of nutrient limitation indicators for P (C:P, N:P, P debt, APA, Fv/Fm), N (C:N, NH4 debt, NO3 debt, Fv/Fm), and Fe (Fv/Fm), as well as photosynthetic efficiency (Fv/Fm) experiments. Nutrient enrichment experiments were also conducted in the nearshore and offshore of the eastern basin of Lake Erie which involved the addition and removal of Fe alone, as well as in combination with P and/or N. Lake Erie nutrient enrichment experiments provided evidence for P, N and Fe colimitation where the addition of Fe with P relieved Fe and P limitation and allowed nitrate (NO3-) assimilation, alleviating N limitation. However, the offshore experiments indicated stronger Fe limitation than the nearshore experiment. Lower chla concentrations in the post-dreissenid nearshore of the eastern basin of Lake Erie may not be due entirely to lower phytoplankton biomass, as photoacclimation of the phytoplankton may also be occurring. Dreissenid grazing effects can be seen in the distribution of dissolved nutrient concentrations between the nearshore and offshore of post-dreissenid Erie. The African Great Lakes are threatened by expanding human populations, resulting in increased nutrient runoff; the consequences of which will depend on the limiting nutrient(s). I found that the nearshore regions of Lakes Malawi and Tanganyika were colimited by N and P, while the offshore regions were colimited by N, P and Fe. The nearshore of Lake Victoria was colimited by light and N, while the offshore was colimited by N, P and Fe. Fe limitation only occurs in the offshore, and positive, significant relationships were found between total dissolved Fe concentrations and cyanobacteria. Continued P and Fe loading to the lakes will create a higher N demand that will result in a shift to N2-fixing cyanobacteria, which has serious consequences to human and ecosystem health as they are a poor nutritive food source and some are potentially toxigenic. The majority of studies conducted on Great Lakes involve offshore sampling, however, the less understood nearshore is where human impacts and activities are concentrated. I discovered there are significant differences between the nearshore and offshore, which has implications for water quality monitoring.

My doctoral thesis challenges the traditional paradigm of phosphorus (P) limitation of phytoplankton communities in freshwaters by suggesting colimitation of P, nitrogen (N), and iron (Fe) in Great Lakes. Oceanographers have recognized Fe, N and P colimitation, and biomass response to Fe is documented in freshwater lakes. I studied African and North American Great Lakes that are similar to large inland oceans. I discovered that Fe is a key nutrient that is often limiting in the offshore, and may explain the dominance of cyanobacteria in nutrient enriched lakes. I also discovered that the nearshore and offshore areas of these large lakes are very different, particularly when invasive dreissenid mussels are impacting the nearshore, as seen in the eastern basin of Lake Erie. As a result of the dreissenids, chlorophyll a (chla) concentrations are significantly lower in the nearshore of Lake Erie, but higher in the nearshore in the three African Great Lakes, as well as pre-dreissenid Lake Erie. The objective of my thesis was to determine the limiting nutrient(s) to the phytoplankton of the Great Lakes Erie, Malawi, Tanganyika, and Victoria in both the nearshore and offshore by measuring the physiological status of the phytoplankton. I also examined how dreissenids affect the distribution of seston and nutrient concentrations between the nearshore and offshore of the eastern basin of Lake Erie. My study design included temporal and spatial surveys in the nearshore and offshore of the four lakes, in which I used a variety of nutrient limitation indicators for P (C:P, N:P, P debt, APA, Fv/Fm), N (C:N, NH4 debt, NO3 debt, Fv/Fm), and Fe (Fv/Fm), as well as photosynthetic efficiency (Fv/Fm) experiments. Nutrient enrichment experiments were also conducted in the nearshore and offshore of the eastern basin of Lake Erie which involved the addition and removal of Fe alone, as well as in combination with P and/or N. Lake Erie nutrient enrichment experiments provided evidence for P, N and Fe colimitation where the addition of Fe with P relieved Fe and P limitation and allowed nitrate (NO3-) assimilation, alleviating N limitation. However, the offshore experiments indicated stronger Fe limitation than the nearshore experiment. Lower chla concentrations in the post-dreissenid nearshore of the eastern basin of Lake Erie may not be due entirely to lower phytoplankton biomass, as photoacclimation of the phytoplankton may also be occurring. Dreissenid grazing effects can be seen in the distribution of dissolved nutrient concentrations between the nearshore and offshore of post-dreissenid Erie. The African Great Lakes are threatened by expanding human populations, resulting in increased nutrient runoff; the consequences of which will depend on the limiting nutrient(s). I found that the nearshore regions of Lakes Malawi and Tanganyika were colimited by N and P, while the offshore regions were colimited by N, P and Fe. The nearshore of Lake Victoria was colimited by light and N, while the offshore was colimited by N, P and Fe. Fe limitation only occurs in the offshore, and positive, significant relationships were found between total dissolved Fe concentrations and cyanobacteria. Continued P and Fe loading to the lakes will create a higher N demand that will result in a shift to N2-fixing cyanobacteria, which has serious consequences to human and ecosystem health as they are a poor nutritive food source and some are potentially toxigenic. The majority of studies conducted on Great Lakes involve offshore sampling, however, the less understood nearshore is where human impacts and activities are concentrated. I discovered there are significant differences between the nearshore and offshore, which has implications for water quality monitoring.

This doctoral thesis examines the challenges to co-management in the Tanzania part of Lake Victoria. The study mainly addresses the Nile perch fishery and uses the fishing communities of Bukoba Rural district, Tanzania as a case study. Co-management in Lake Victoria is defined as the sharing of the management responsibilities between the state and the fishing communities. Co-management was adopted in the Lake Victoria fisheries on the understanding that it has the capacity to provide space in which the poor resource users could be empowered to sustainably manage their resource base. The assumption was that the sharing of the management responsibilities between the state and the community of users would have led to equity in resource access, poverty reduction and resource sustainability. Thus, reducing the role of the state and enhancing that of the communities was seen as a solution to the problems of poverty and illegal fishing that are threatening the sustainability of the fishery and the fishers dependent on it. However, in spite of these proclaimed efforts, illegal fishing and poverty in Lake Victoria remain major threats to the long-term sustainability of the fishery, a fact that is raising questions on the efficacy of co-management in Lake Victoria. These questions have particularly focused on the co-management model and the neo-liberal ideals that underlie it, namely decentralization, participation and accountability. The central argument in this thesis, however, is that co-management performance in Lake Victoria is to a large extent shaped by the complex international, national, and local context in which it is implemented and which in turn shapes the problems of poverty and illegal fishing that co-management is supposed to address. The study concludes that the international and national politics behind the Nile perch fishery intersect with the cultural and social context in which the fishery is embedded to shape co-management performance at the local level. For analysis, the study applies a multi-level approach and draws insights from the common pool resources theory, the actor-oriented approach, the entitlement framework, and the theory of the state. Detailed interviews across scale, secondary data, policy documents, and laws, supported by quantitative data are the methods applied.

As we celebrate all the dynamic and dramatic improvements in human health care in the 21st century, life in much of Africa begins with and is sustained with the support of traditional medicinal knowledge. Research on traditional medicinal knowledge (TMK) is extensive, but rather few studies have been written about Traditional Healers' (THs') own perceptions about TMK and practices in relation to changing societal dynamics. The aim of this thesis is to examine how THs perceive on going socio-spatial transformation, including contemporary processes of urbanization, migration, commercialization and commodification of TMK, as well as changing dynamics of learning and knowledge systems between generations and genders and how these affect their medicinal healing practices in time and space. The thesis consists of four main empirical chapters, which derive from different data sources including literature, documentation review and qualitative interview material. The findings in this thesis can be summarised as follows: First that TMK today exists side by side with modern health systems, in what are seen as complex patterns of medical pluralism that provide evidence of an evolving role the TH plays in primary health care, in the rural and urban space. Youthful migrating population dynamics that are linked to historical processes, have effectively carved an emerging cross-sectoral role of the TH in the formal space. Secondly the developing legislation on IPR and ABS in parallel with the representation of an earlier official formal governance around TMK in Tanzania; and the difference in the sectors where TMK is anchored in the two contexts, could have paved way to some earlier collaborative mechanisms, that today provide space to enable a more natural engagement between formal and informal organizations involved in the governance of TMK in Tanzania. Thirdly, the practical ways in which TMK learning processes, which are characterized by learning systems in place, being sent and visiting sacred places that are lived by an apprentice over a number of years, have increasingly come under pressure. Fourthly the thesis shows approaches by THs, encouraging the youth to access conventional medicinal education followed by, or in parallel with TMK learned through traditional pedagogies employed by the THs themselves. The youth’s keen interest in learning TMK is seen to increase when they view improved livelihood possibilities due to the commercialization of medicinal plants. The future of TMK learning processes may be limited unless incentives are put in place for the youth regarding their future livelihoods. Fifth, gendered and generational dimensions suggest that older and some younger female THs reemphasize the values of the gift and TMK in a climate of increased commodification and commercialization of TMK, where TMK increasingly meets neoliberal processes, engaging an alternative paradigm than the gift economy, where a predominance of male TH’s in the urban space and places, increasingly define the diversification of the TMK livelihoods. The gift provided by a higher power and which is embedded in a particular cosmological view, to be used as a social service to help the community, is increasingly evolving as an emerging tested force in a changing ideological climate, with an increasing awareness of commodification, commercialization, IPR and ABS issues surrounding TMK. It implies awareness in relation to the increased benefits of commoditized and commercialized medicinal plant knowledge (which THs hold) for other individuals and institutions. The TH profession and TMK is seen as entering a contested IPR/ABS arena at a time when increasingly socio-spatial transformations are modifying its role from that of a gift to an owned commodity. However while the practice of TMK has changed over time and space, presenting new challenges as well as opportunities, it is also seen as a threat that anyone today can sell and market TMK products.

This thesis analyses the architecture of the Royal Victoria College (Bruce Price, 1896--1899), a purpose-built women's residential college of McGill University, Montreal, and its first extension (Percy Nobbs, 1930--193 1), as material evidence of the rhetorical construction and negotiation of gender. A contextual analysis of the original RVC reveals the gender significance of the building's relationship to its affiliate institution (McGill), to an urban geography (Phillips Square), and to a commercial typology (the railway hotel), while a spatial analysis examines the significance of its women occupants as 'architects', and of changes to the building over time. The thesis concludes that the building served as an important site in turn-of-the-century gender negotiations---one that helped to contest "separate spheres" rhetoric and that stands as evidence of women's active participation in the shaping of spatial relations and social identities.

This thesis presents the distribution of strong wind and wind pressure in the Greater Victoria area associated with winter mid-latitude cyclones based on climate data from the School-Based Weather Station Network during 6 selected days in the winters of 2006, 2007 and 2008. The objectives of this study are i) to test whether synoptic conditions favourable to severe mid-latitude cyclonic storms that are well described in the literature were associated with the selected storms, ii) to determine the time patterns of high wind speed and its direction and maximum gusts, iii) to test necessity of considering the spatial variation in air density and its controls in general assessments of the spatial variation in wind pressure and wind damage potential in the local area, iv) to identify potential areas susceptible to wind damage. Observations taken every second were from Davis Vantage Pro2 TM Plus weather stations located on the southern edge of school building roofs. Thirty-minute means and gust wind speeds were used. All six storms went north of Victoria. The synoptic conditions associated with the selected mid-latitude cyclones agreed with the ones described in literature. Strongest winds at most stations were generally from the southwest, and multiple wind speed peaks were found. The daily iii  maximum gust wind speeds were found before and/or after the highest mean wind speed peak. The spatial variation in air density and its controls were found to be negligible. Although there are a number of interacting causes of the distribution, strongest winds were at stations with smooth surrounding surfaces, close to the southern shoreline, on exposed slopes and/or near relief constrictions. The area with greatest wind speeds and damage potential was found from the east of downtown extending to Lansdowne Middle School. This study provides new knowledge of winds in the Greater Victoria area and contributes to people’s better response to wind storms, land use planning and forecasting severe windstorms. / Graduate / 0368 / mmatsuda@uvic.ca

From the mid 1850s through the early 1900s, the white middle and upper class inhabitants of British Columbia persevered in their attempts to solve the ‘servant problem’ and to re-create the British domestic sphere in a new land. Some families emigrated with their British servants in tow. There were repeated efforts to import English girls and women en masse. And many employers were obliged to tolerate ‘strangers’ (Aboriginal and Chinese servants) in their homes. British Columbia’s peculiar ‘servant problem’ ensured that the Imperial vision of employer-servant relations and domestic order could not be exactly reconstructed.

Communities such as the Capital Regional District (CRD) of Vancouver Island (BC) currently struggle to meet the transportation needs of older adults and other vulnerable populations, particularly in terms of resources and funding. In the future, these challenges will continue given the current demographic shift and the existing structure of the transportation system. Since the provision of transportation is an important element for the quality of life of older adults, it is important to consider solutions that promote a more effective use of transportation opportunities to ensure health, self-sufficiency and community connectedness amongst this population. This research study examines one solution to the issues surrounding the provision of transportation to older adults. A Coordinated Accessible Transportation (CAT) approach supports the pooling and sharing of existing transportation resources within a formalized framework, and aims to provide safe, efficient and affordable transportation options to older adults. This research focuses on the identification of barriers and incentives to the implementation of a CAT program. This project used a qualitative, case study approach to reach its objectives. Twenty-three semi-structured interviews were conducted with stakeholders involved in the provision of transportation. A Program Logic Model (PLM) was used to organize and analyze the information gathered. In summary, although aware of the need for a new approach, transportation providers in the CRD are reluctant to change the current system without active governance being in place at all levels. The results of this research provide a `blueprint' for the implementation of a CAT program and have relevance for other Canadian communities facing similar challenges.