The presented work deals with preparation of test samples filled microparticles of mica and nanoparticles of silicon dioxide (SiO2). Monitors the impact of climatic conditions and filler on electrical properties of composite materials. Electrical properties of composite materials are measured by dielectric relaxation spectroscopy. Test samples influence of epoxide resin TSA 220S and polyester resin DOLPHON XL 2102. Varnishes are modified with different weight percentage of nanoparticles of silicon dioxide, or micronized mica. The results of this project are frequency dependencies of parts of complex permittivity and their evaluation.

Thesis (M. Phil.)--University of Hong Kong, 1982.

Interleukin-2 activates lymphocytes to become highly cytotoxic for a wide range of tumour cell types in vitro (Iymphokine activated killer or LAK cells), and in animal models. However, only limited therapeutic benefit was observed in clinical trials of LAK cell therapy. This project aimed to investigate the molecular and cellular interactions involved in the production and effector functions of LAK cells, to identify factor(s) which might be responsible for the poor clinical responses observed in LAK cell therapy. Tumour cell lines were heterogeneous in their response to killing by cytokines (TNFα, LT, IFNγ and IL-1β), and purified monocytes or lymphocytes, but were consistently highly sensitive to LAK cell cytotoxicity. Autologous monocytes and lymphocytes were not killed by LAK cells, in contrast to human umbilical vein endothelial cells and fibroblasts. Supernatants from LAK cells were considerably less cytotoxic than the effector cells, and physical separation of effector and target cells resulted in inhibition of killing. Lymphocyte and LAK cell cytotoxicity was associated predominantly with the CD8+ (cytotoxic T-cell) lymphocyte sub-population, and was significantly inhibited by anti-TNFα and anti-LT, demonstrating that these cytokines were the primary effector molecules in this system. LAK cells and A375 melanoma cells showed procoagulant activity, predominantly via the tissue factor pathway, and LAK cells also possessed surface factor V. In addition, A375 cells were highly fibrinolytic. Tumour cell killing by LAK cells was inhibited by plasma, and further experiments determined that polymerised fibrin, but not fibrin monomer was responsible. From these results it was suggested that culture of small numbers of cells from tumour biopsies, and the determination of their sensitivity to cytotoxic drugs, cytokines and effector cells may lead to more effective treatment protocols for immunotherapy of individual tumours. In order to enhance the efficacy of immunotherapy, further in vivo research is required to elucidate the interactions between immune effector cells and the coagulation/fibrinolytic systems.

Humboldt-Univ., Diss.--Berlin, 2004.

Thesis (doctoral)--Humboldt-University, Berlin, 2004.

This diploma thesis deals with the issue of the degree of damage done to the vehicle‘s body paint varnish by bird droppings; with respect to the influence of the pollution time on the thickness of the layer of lacquer. The first chapter deals with the importance of varnish and other layers of body protection, types of body varnish damage, painting technology, lacquer defects, bird droppings and its effects on the varnish of the vehicle‘s body. The second chapter is dedicated exclusively to the description, planning and measurement of the coated layers on the vehicle’s body contaminated with bird feces.

This work consider with monitoring components of komplex permittivity at frequency section of electro-insulation material in process of wetting by dielectric relaxation spectroscopy.

This master's thesis focuses on composite systems based on varnish combined with different filler ratios of micronized mica. The aim is to analyse impact of the filler on dielectric properties of the varnish, i.e. relative permittivity and loss factor as functions of frequency as well as charging and discharging currents as functions of time. Dielectric mixture formulas are applied on relative permittivity values. Calculated and measured values are compared.

L'utilisation d'organismes génétiques modèles a permis l'investigation à grande échelle de mécanismes cellulaires et moléculaires pour la biologie. La larve de poisson zèbre (Danio rerio) est un organisme vertébré modèle simple qui présente plusieurs avantages pour identifier les bases moléculaires et pharmacologiques du développement et du comportement à travers des cribles génétiques et chimiques respectivement. Plusieurs paradigmes expérimentaux reposent sur une caractérisation précise du phénotype comportemental associé avec un génotype ou l’application d’une drogue. Ce processus de phénotypage bénéficierait d'une compréhension globale des manœuvres possibles afin de caractériser précisément le comportement globale de larves observé au cours d'une expérience. L'analyse sur plusieurs minutes de la locomotion spontanée des larves de poisson zèbre en groupe fournit un cadre idéal pour atteindre ces objectifs.Si l'analyse manuelle du comportement animal est possible pour l'observation de manœuvres isolées et stéréotypées, elle n’est pas compatible avec une approche à grande échelle. A partir de cinq jours, la larve de poisson zèbre se meut sous forme de bouffées natatoires qui arrivent à haute fréquence et sont séparées par quelques secondes. La difficulté d'observation à haute fréquence et d'analyse de la locomotion en bouffée rend une analyse manuelle simple à partir de séquences vidéos du comportement impossible. Le développement récent de caméras rapides avec acquisition en mode continu, couplé aux avancées en informatique et en intelligence artificielle rend possible une analyse automatique du comportement. Les systèmes commerciaux actuels permettent des enregistrements sur des longues durées mais sans capturer la complexité et la diversité des mouvements réalisés. Nous avons donc créé ZebraZoom, notre système d'analyse haut débit permettant de suivre, de quantifier et de catégoriser le comportement spontané de chaque larve de poisson zèbre au sein d’un groupe.Nous avons monté un dispositif expérimental qui consiste à placer sept larves de poisson zèbre âgées d'entre cinq et sept jours dans huit boîtes de pétri. Les boîtes de pétri sont disposées sur une table lumineuse et une caméra rapide positionnée au-dessus enregistre le comportement spontané à 337 Hz pendant quatre minutes. Une fois la vidéo acquise, ZebraZoom localise automatiquement chacune des huit boîtes de pétri, puis suit tête et queue de chacun des animaux sur l'ensemble de la vidéo, grâce à des procédures de vision par ordinateur. ZebraZoom identifie automatiquement chaque bouffée natatoire. Le phénotypage du comportement a été réalisé grâce à deux méthodes complémentaires : l'extraction de paramètres globaux caractérisant la dynamique des mouvements et la catégorisation automatique des mouvements en différents manœuvres stéréotypées. Nous avons utilisé l’analyse des paramètres globaux afin de caractériser l'effet de drogues agissant sur les récepteurs de neurotransmetteurs et l’analyse d’un mutant aveugle. La catégorisation automatique des mouvements est réalisée grâce a des procédures d’apprentissage automatique (« Machine Learning »). Nous avons illustré l’utilité de cette catégorisation pour étudier les interactions entre larves à ces stades précoces. En conclusion, notre programme ZebraZoom permet de réaliser un phénotypage automatique et complet, et cette approche pourra être appliquée dans d'autres systèmes et contextes expérimentaux. / The use of simple genetic model organisms has allowed large-scale investigation of cellular and molecular mechanisms in biology. The zebrafish larva (Danio rerio) is a simple vertebrate model with several advantages for the identification of the molecular and pharmacological basis of development and behavior thanks to genetic and chemical screens. Several experimental paradigms rely on a precise behavioral characterization associated with a genotype or a drug application. This phenotyping process would benefit from a global comprehension of all possible zebrafish larvae maneuvers in order to precisely characterize the global behavior of larvae observed during an experiment. The analysis over multiple minutes of the spontaneous locomotion of zebrafish larvae in a group provides an ideal framework for achieving these goals.The manual analysis of animal behavior is possible for the observation of stereotyped and isolated maneuvers, but it is not compatible with large-scale approaches. After five days, the zebrafish larva moves in burst of movement occurring at high frequency and separated by a few seconds. The difficulty of observation of high-frequency maneuvers makes a manual analysis of videos impossible. The recent development of high-speed cameras, coupled with advances in computer science and artificial intelligence, make an automatic analysis of behavior possible. The available commercial solutions allow long acquisitions but without capturing all the complexity and diversity of the movements. We thus created ZebraZoom, our high-throughput analysis system which makes it possible to follow, quantify and categorize the spontaneous behavior of all zebrafish larva in a group.We built an experimental setup which consisted in placing seven 5- to 7-day old zebrafish larvae in eight petri dishes. The petri dishes were placed on a light table and a high-speed camera was placed above the setup and allowed to record the spontaneous behavior at 337 Hz during four minutes. Once the video was acquired, ZebraZoom localized automatically each of the eight petri dishes and followed the head and the tail of each of the animals on the entire video, thanks to computer vision methods. ZebraZoom then identifies automatically all burst of movement.The behavior phenotyping was performed thanks to two complementary methods: the extraction of global parameters characterizing movement dynamics and the automatic categorization of movements into stereotyped maneuvers. We used global parameters analysis to characterize the effect of drugs acting on neurotransmitters receptors and to analyze the behavior of a blind mutant. The automatic categorization of movements was carried out thanks to machine learning procedures. We showed the utility of this categorization method in studying interactions between larvae at early stages. In conclusion, our ZebraZoom program makes it possible to carry out an automatic and complete phenotyping, and this approach could be used in other systems and experimental contexts.

Esta pesquisa pretende, no contexto da expansão do protagonismo judicial (principalmente sobre o aspecto da judicialização das políticas públicas), estudar as audiências públicas realizadas pelo Supremo Tribunal Federal e seus efeitos na realização do direito à saúde que, por ser um direito social, se concretiza, principalmente, por meio de políticas públicas. O objetivo é verificar se as audiências públicas realizadas sobre o tema (audiências sobre a judicialização da saúde, sobre o programa Mais Médicos e sobre a internação com diferença de classe no SUS) foram satisfatórias no sentido de serem capazes de criar uma sistemática de atuação para o Poder Judiciário nos casos que envolvem o controle de políticas públicas de saúde. Para tanto, realizamos a análise de obras teóricas sobre a judicialização dos conflitos, o estudo de casos concretos envolvendo a efetivação da saúde e dividimos a dissertação em quatro partes. Na primeira parte (capítulo um), abordaremos os limites metodológicos do direito positivo tradicional no que se refere à efetivação dos direitos sociais, no contexto da expansão do protagonismo judicial e da ascensão do neoconstitucionalismo, apontando como reflexo a estes desafios a institucionalização das audiências públicas. No segundo capítulo trataremos do papel que as audiências públicas assumiram no STF, destacando de forma crítica a apropriação do instituto como um canal de diálogo democrático com a sociedade civil, asseverando que sua utilização é discricionária, pontual e seletiva, atendendo assim, principalmente, a uma busca por maior legitimidade decisória. No terceiro capítulo, tendo em vista as dificuldades inerentes para a efetivação do direito à saúde e o caráter limitado que as audiências públicas assumiram junto à Corte Suprema, analisaremos os efeitos do instituto na criação do que poderíamos chamar de política judiciária de saúde. Por fim, no quarto capítulo, apontaremos para caminhos que visam a superação da lógica compensatória e individualista da aplicação do direito à saúde, por meio da valorização de um verdadeiro diálogo do Judiciário com a sociedade civil, alcançada somente com a realização de audiências públicas verdadeiramente deliberativas. / In the context of the expansion of judicial protagonism (especially on the aspect of judicialization of public policies), this research aims to study the Supreme Court public hearings and the effects on social rights awereness. The goal is to verify if the public hearings regarding \"judicalization of health,\", \"Mais Médicos\" program and \"admission to differences classes in the SUS\", were able to create a pattern of the Judiciary acting in cases involving control of public health policies satisfactorily. The research compreends the analysis of theoretical works on the judicialization of conflicts, case studies involving the effectiveness of health and is divided in four parts. On the first chapter the analysis will be on the methodological limits of traditional positive law, in the context of the expansion of the judicial role and neoconstitutionalism rise. On the second, the analysis will be on the role of the public hearing at the Supreme Court level. The emphases is critically pointing the institute\'s institucionalization as a democratic channel of dialogue with civil society, stressing that its use is discretionary, timely and selective. In the third chapter, given the difficulties inherent to the application of the right to health and the limited nature that the public hearings have taken by the Supreme Court, we will analyze the effects of public hearings in creating what we might call \"judicial policy of health\" . Finally, in the fourth chapter, we will point to ways to overcome the compensatory and individualistic logic of the application of the right to health through the promotion of a true judicial dialogue with civil society.