• Refine Query
  • Source
  • Publication year
  • to
  • Language
  • 932
  • 737
  • 288
  • 106
  • 35
  • 35
  • 35
  • 35
  • 33
  • 26
  • 23
  • 12
  • 10
  • 10
  • 8
  • Tagged with
  • 3556
  • 738
  • 639
  • 593
  • 553
  • 533
  • 332
  • 316
  • 316
  • 251
  • 242
  • 184
  • 183
  • 161
  • 156
  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
531

Tunable multiwavelength picosecond pulses generated from a fabry-perot laser diode.

January 1998 (has links)
by Sui-Pan Yam. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references. / Abstract also in Chinese. / Acknowledgements --- p.V / Abstract --- p.VI / Chapter Chapter 1 --- Introduction --- p.1-1 / Chapter 1.1) --- Tunable Multi-Wavelength Optical Sources --- p.1 -1 / Chapter 1.2) --- All-Optical Switching --- p.1 -3 / Chapter 1.2.1) --- Nonlinear Effect / Chapter 1.2.2) --- Special Design of the Laser Structure / Chapter 1.2.3) --- Self-Injection Seeding of Fabry-Perot Laser Diode / Chapter 1.3) --- About This Project --- p.1-6 / Chapter Chapter 2 --- Basic Theory --- p.2-1 / Chapter 2.1) --- Mechanism of Gain-Switching --- p.2-1 / Chapter 2.1.1) --- General Description / Chapter 2.1.2) --- "Optical Pulsewidth, Spectra, and Frequency Chirping of Gain-Switched Pulses" / Chapter 2.2) --- Mechanism of Self-Injection Seeding --- p.2-8 / Chapter 2.2.1) --- General Description / Chapter 2.2.2) --- Dynamics of Single-Mode Formation / Chapter 2.2.3) --- Frequency Evolution of the Laser Diode for Cavity Mode Selection / Chapter 2.2.4) --- Turn-On Delay Time Jitter (TOJ) / Chapter 2.3) --- Mechanism of Injection Seeding --- p.2-17 / Chapter 2.3.1) --- General Description / Chapter 2.3.2) --- The Model of Weak Injection / Chapter 2.3.3) --- The Model of Strong Injection / Chapter Chapter 3 --- Single- and Multi-wavelength Optical Pulses Generated by a Diffraction Grating --- p.3-1 / Chapter 3.1) --- Introduction --- p.3-1 / Chapter 3.2) --- Basic Principle --- p.3-2 / Chapter 3.3) --- Experimental Setup --- p.3-5 / Chapter 3.4) --- Results and Discussion --- p.3-7 / Chapter 3.4.1) --- Spectral Characteristics Analysis / Chapter 3.4.2) --- Individually Access of the Four-Wavelength Output / Chapter 3.4.3) --- The Optical Pulsewidth Characteristics / Chapter 3.4.4) --- Discussion / Chapter 3.5) --- Summary --- p.3-14 / Chapter Chapter 4 --- Using a Highly Dispersive Fiber for Tunable Multi-Wavelength Pulse Generation --- p.4-1 / Chapter 4.1) --- Introduction --- p.4-1 / Chapter 4.2) --- Basic Principle --- p.4-2 / Chapter 4.3) --- Experimental Setup --- p.4-5 / Chapter 4.4) --- Experimental Results --- p.4-7 / Chapter 4.4.1) --- Spectral and Temporal Characteristics / Chapter 4.4.2) --- Wavelength Tuning / Chapter 4.4.3) --- Individually Access of Two Wavelength Channels / Chapter 4.4.4) --- Multi-Wavelength Generation / Chapter 4.5) --- Summary --- p.4-13 / Chapter Chapter 5 --- Comparison of Two Self-Seeding Configurations --- p.5-1 / Chapter 5.1) --- Introduction --- p.5-1 / Chapter 5.2) --- Polarization Sensitivity --- p.5-1 / Chapter 5.3) --- Stability --- p.5-2 / Chapter 5.4) --- Tunability --- p.5-2 / Chapter 5.5) --- Simplification --- p.5-3 / Chapter 5.6) --- Summary of the advantages and disadvantages of Two Configurations --- p.5-4 / Chapter Chapter 6 --- All-Optical Wavelength Switching achieved by Self-Seeding and External Injection-Seeding --- p.6-1 / Chapter 6.1) --- Introduction --- p.6-1 / Chapter 6.2) --- Experimental Setup --- p.6-2 / Chapter 6.3) --- Results and Discussion --- p.6-4 / Chapter 6.3.1) --- Spectral Characteristics / Chapter 6.3.2) --- The Optical Pulsewidth / Chapter 6.3.3) --- The Optical Switching Behaviors / Chapter 6.3.4) --- The Detail Information of Switching / Chapter 6.3.5) --- Optical Power / Chapter 6.4) --- Summary --- p.6-10 / Chapter Chapter 7 --- A Novel Self-Injection Seeding Scheme --- p.7-1 / Chapter 7.1) --- Introduction --- p.7-1 / Chapter 7.2) --- Basic Principle --- p.7-2 / Chapter 7.3) --- Experimental Setup --- p.7-9 / Chapter 7.4) --- Results and Discussion --- p.7-11 / Chapter 7.4.1) --- Spectral and Temporal Characterizations of Two-Wavelength Switching / Chapter 7.4.2) --- Different Wavelength Selection / Chapter 7.4.3) --- Operation Frequency Against the Fiber Length / Chapter 7.4.4) --- Multi-Wavelength Generation / Chapter 7.5) --- Discussion --- p.7-20 / Chapter 7.6) --- Summary --- p.7-22 / Chapter Chapter 8 --- Comparison of Switching Methods --- p.8-1 / Chapter 8.1) --- Introduction --- p.8-1 / Chapter 8.2) --- Switching between Self-Seeding and Injection-Seeding --- p.8-1 / Chapter 8.3) --- Switching by Self-Seeding of a F-P Laser Diode --- p.8-2 / Chapter 8.4) --- Summary --- p.8-3 / Chapter Chapter 9 --- Conclusion --- p.9-1 / References / Figure Captions / Appendix 一 Equipment Descriptions / List of Accepted and Submitted Publications
532

Development of a process for characterization of Nd:YAG crystals

Bronski, Mark T 09 April 2003 (has links)
The objectives of this thesis were to develop a methodology for the measurement of laser beam characteristics from a single cavity laser and to establish a preliminary guideline that would determine which crystals were acceptable for use in production of laser devices. These objectives were achieved by developing the experimental procedures and by statistical analysis of the data obtained. However, additional future work is needed to independently confirm the results of this thesis. Efficient and reliable operation of a lamp-pumped Nd:YAG laser is highly dependent on the crystal from which the beam is derived. However little attention is given to the quality of the laser beam produced by each crystal. Although many factors influence the output beam, the power dependent focal length is of particular importance. Unfortunately, direct measurement of the crystal focal length is not possible with a Nd:YAG laser beam. This is because the single cavity laser functions as both a resonator and amplifier simultaneously. Therefore, a method was developed that measured the caustic of the laser beam after it had emerged from the resonator and been focused by means of a focusing element. The caustic of the beam was analyzed utilizing a beam analyzer that calculated the beam focusability factor and the beam waist size. From this information, the waist diameter at the outcoupler mirror was calculated using Gaussian beam propagation principles. A resonator model was developed based on the self-repeating ABCD matrix that allowed for the determination of the induced thermal lens based on the input power. Several approaches to model the thermal lensing effect were taken, each with increasing complexity. As a result, three parameters were evaluated with the intention of using one or more as a means to classify good and bad crystals. They were the crystal sensitivity factor, the beam focusability factor, and the beam waist size at the measurement plane. Calculation of the crystal sensitivity factor, M^-1, was based on the developed resonator model and numerous approximations of the crystal behavior. Thus, after calculating the M-1 factor as a function of input power, no distinguishable pattern was seen. However, the beam focusability factor and the beam size, both showed distinct regions that separate good and bad crystals. Statistical analysis performed on the data supports a preliminary conclusion that these two parameters may be used as a quality control measure. These parameters are measured using existing internationally accepted procedures and are therefore the best currently available tools for determination of the quality of Nd:YAG crystals.
533

Laser de baixa intensidade no reparo tecidual do segundo molr após cirurgia de terceiro molar : ensaio clínico randomizado duplo cego /

Oliveira, Rúbia Gomes de. January 2018 (has links)
Orientador: Michelle Bianchi de Moraes / Coorientador: Fernando Vagner Raldi / Banca: Andrea Carvalho De Marco / Banca: Fábio da Silva Matuda / Resumo: A exodontia de terceiros molares inferiores é uma prática comum nas clínicas odontológicas, porém o pós-operatório inerente a esta cirurgia apresenta dor, edema e trismo. Algumas das posições de inclusão do terceiro molar podem acarretar comprometimento periodontal nos segundos molares adjacentes. Com isso o presente trabalho buscou desenvolver através deste estudo clínico controlado, um protocolo com o laser de baixa intensidade para aplicação no pós-operatório das exodontias de terceiros molares mandibulares, de modo a minimizar os sinais e sintomas e melhorar os parâmetros periodontais dos segundos molares adjacentes. O laser empregado foi o Photon Laser III - DMC, São Carlos, SP/Brasil - como meio de condução o vermelho, com comprimento de onda 660 nm, potência útil de 30 mW, meio ativo AsGaAl. Sessenta pacientes de ambos os gêneros, com necessidade de exodontia dos terceiros molares, em classificações padrões de posição, foram randomizados em três grupos para análise - Grupo I: 10J/cm2, Grupo II: 30J/cm2 e Grupo III: Sham, e acompanhados pelo período de 6 meses. A análise do trismo e edema facial no pós-operatório e do nível clínico de inserção encontraram diferenças estatísticas significantes entre os grupos que receberam a terapia laser quando comparado ao grupo sham. Portanto, a utilização do laser de baixa intensidade como terapia adjuvante após a exodontia de terceiros molares demonstrou efetividade. Sendo que o Grupo I se destacou, após seis meses de avaliação. / Mestre
534

Generation and characterization of tunable multi-wavelength continuous-wave and picosecond-pulsed outputs from a semiconductor laser. / CUHK electronic theses & dissertations collection

January 1998 (has links)
by Ka-Suen Lee. / "June 1998." / Thesis (Ph.D.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references. / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.
535

Synchronization dynamics of dual-mode solid-state and semiconductor DFB lasers under frequency-shifted feedback : applications to microwave photonics / Dynamiques de synchronisation de lasers bifréquence à état solide et DFB soumis à une réinjection décalée en fréquence : applications en photonique micro-onde

Thorette, Aurélien 30 November 2018 (has links)
Le contrôle de la différence de fréquence entre deux lasers est un défi transversal à de nombreux domaines de la photonique, que ce soit dans un but de génération hétérodyne d'un battement micro-onde de grande pureté, ou pour des expériences de métrologie ou de télécommunication. L'avancée des connaissances sur la dynamique de lasers soumis à divers couplages a permis le développement de méthodes de stabilisation basées sur l'injection optique. Nous étudions ici théoriquement et expérimentalement un mécanisme appelé réinjection décalée en fréquence (RDF), qui permet dans des situations variées de contrôler précisément la différence de fréquence entre deux lasers. Dans un premier temps, la méthode RDF est appliquée à un laser à état solide bi-polarisation bi-fréquence Nd:YAG afin de verrouiller en phase ses deux modes de polarisation orthogonaux. Le développement d'un modèle type «rate equations» en bonne adéquation avec les expériences a aussi permis de mettre en lumière un certain nombre de régimes de synchronisation partielle dits de phase bornée. De plus, nous montrons que cet état peut subsister en présence d'oscillations chaotiques de l'intensité et de la phase. Le comportement du laser sous RDF est étudié pour différentes valeurs du désaccord de fréquence, du taux d'injection, du retard éventuel, et du couplage inter-modes. Enfin, la nécessité d'inclure un couplage phase-amplitude (facteur de Henry non-nul) dans le modèle a mené au développement d'une méthode pour mesurer ce coefficient habituellement négligé dans les lasers solides. Le mécanisme de stabilisation par RDF est ensuite appliqué à un composant semiconducteur original contenant deux lasers DFB sur InP. Malgré une plus grand complexité du schéma de couplage, et la présence de retards effectifs importants, il reste possible de synchroniser en phase ces lasers. Des bandes d'accrochages liées au retard sont observées, et reproduites à l'aide d'un modèle numérique. Ce dernier permet aussi de déterminer les conditions de fonctionnement minimisant l'influence de paramètres expérimentaux non maîtrisés. Enfin, ce système, permettant de contrôler une phase micro-onde sur porteuse optique, peut être intégré dans une boucle résonante de type oscillateur opto-électronique (OEO) qui ne nécessite pas de référence externe. On réalise un oscillateur micro-onde sur porteuse optique auto-référencé, à bande latérale unique, ayant des performances encourageantes. Dans ce cadre, il semble que la plupart des techniques développées pour les OEO puissent être réinvesties. / The control of the frequency difference between two lasers is a cross-cutting challenge in many fields of photonics, either for the generation of high-purity heterodyne microwave beatnotes, or in metrology and telecommunication experiments. The advances of the comprehension of laser dynamics under various couplings has allowed to develop stabilization methods based on optical injection. We study here theoretically and experimentally a mechanism called frequency-shifted feedback (FSF), which allows to precisely control the frequency difference between two lasers in several situations.First, the FSF method is applied to a dual-frequency dual-polarization solid-state Nd:YAG laser, in order to lock the phases of its two orthogonal polarization modes. A model of rate equations is used to precisely describe the experiment, and allows to highlight partial "bounded phase" synchronization regimes. Furthermore, we show that in some cases this synchronization can subsist even with chaotic oscillations of the intensity and phase. The behavior of the laser under FSF is studied for varying values of the frequency detuning, injection rate, possible injection delay, and mode coupling in the active medium. Finally, we find that the inclusion of a phase-amplitude coupling (non-zero linewidth enhancement factor) is needed in the model to account for experimental observation. This leads to the development of an ad-hoc technique to measure the low value of this usually neglected factor in solid-laser lasers.The FSF stabilization mechanism is then applied to a custom semiconductor component embedding two DFB lasers overs InP. In spite of a more complex coupling scheme and the large effective delays into play, phase locking of the two lasers is possible. Due to the delay, locking bands appear when the detuning changes, and this behavior can be replicated using a numerical model. This model also permit to determine working conditions minimizing the influence of uncontrolled experimental optical feedback phases. Finally, as this system allows to control a microwave phase over an optical carrier, it can be integrated in a resonant loop not unlike an opto-electronic oscillator (OEO). We realized an oscillator generating a self-referenced, single sideband microwave signal over an optical carrier, with encouraging phase noise performances. In this case, it seems that most of the techniques that exist for standard OEO can be reused.
536

New schemes of picosecond pulse generation with broad tunability in wavelength and repetition rate. / CUHK electronic theses & dissertations collection

January 2005 (has links)
Active mode locking is one of the simplest ways to generate picosecond pulses at gigahertz repetition rates. In my works, I demonstrate the generation of picosecond pulses with a center-wavelength spanning from 1489nm to 1589nm using a polarization maintaining fiber loop mirror filter (PMF-LMF) in a mode-locked semiconductor optical amplifier (SOA) ring laser. By applying the SOA gain shifting technique and with the help of the controllable transmission ratio of the PMF-LMF, the tuning range of the output wavelength can be extended. By applying the technique of dispersion tuning, electrical wavelength tuning can be achieved across a range of 100nm. / Compared to the active mode-locking method, the regenerative mode-locking is very convenient because it does not require any external source for modulation and is proved to be more robust against fluctuations in ambient temperature. We demonstrate a 10-GHz regeneratively mode-locked fiber laser using a PMF-LMF. The operating frequency is determined by the free-spectral-range of the PMF-LMF and the component is extracted optically from the ring laser output. / In addition, we also demonstrate a simple technique to generate wavelength tunable picosecond pulses at adjustable repetition rate without using electrical or optical RF filter to extract the radio frequency (RF). The RF signal for mode locking is generated from a Fabry-Perot laser diode (FP-LD) under optical injection. The output frequency can be varied by adjusting the biasing current of the FP-LD. (Abstract shortened by UMI.) / Picosecond optical pulse sources with broad tunability and various repetition rates are key elements for applications in wavelength- and time-division multiplexed optical transmission systems. Mode-locking is one of the main techniques for the generation of optical pulses with high repetition rate picosecond pulse trains. This thesis presents our research efforts in high repetition rate optical pulse generation using active and regenerative mode-locking techniques, and a self-starting approach. We also demonstrate the application of harmonic mode locking in all-optical clock recovery from NRZ data. / Tang Wing Wa. / "August 2005." / Adviser: C. T. Shu. / Source: Dissertation Abstracts International, Volume: 67-07, Section: B, page: 4015. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references. / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract in English and Chinese. / School code: 1307.
537

Distributed feedback dye-doped sol-gel silica lasers. / CUHK electronic theses & dissertations collection

January 2001 (has links)
Zhu Xiao Lei. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (p. 116-121). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.
538

Caracterização numerica e experimental da atenuação da radiação laser em espuma metalica

Junqueira, Silvio Luiz de Mello 01 July 1996 (has links)
Orientador: Jose Luis Lage, Luiz Fernando Milanez / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Mecanica / Made available in DSpace on 2018-11-08T18:36:48Z (GMT). No. of bitstreams: 1 Junqueira_SilvioLuizdeMello_D.pdf: 23366873 bytes, checksum: 40ff350275cdc60184b7883f90658798 (MD5) Previous issue date: 1996 / Resumo: O presente trabalho trata do estudo teórico e experimental dos efeitos térmicos causados pela aplicação da radiação laser sobre meios porosos e objetiva a determinação do coeficiente de atenuação de um meio poroso imerso em fluido. O modelamento matemático proposto utiliza a técnica dos volumes finitos, para resolver numericamente a equação do transporte do calor em coordenadas.cilíndricas. Um sistema de aquisição de dados baseado no conceito de instrumentos virtuais é elaborado para analisar o processo de aquecimento pela radiação de um laser de Argônio sobre uma matriz porosa de Alumínio 6101 imersa em quatro fluidos diferentes: ar, água, óleo polialfaolefina e Mercúrio. Uma metodologia inversa, estabelecida pela comparação de resultados numéricos e experimentais, é empregada para obter o coeficiente de atenuação da espuma metálica saturada. A análise inclui o emprego de duas metodologias de cálculo da condutividade térmica equivalente. Uma correlação entre os coeficientes de atenuação e um número de Prandtl equivalente do meio poroso é estabelecida / Abstract: In the present work, an investigation of thermal effects due to laser application is accomplished in order to determine the attenuation coeflicient of a porous matrix immersed in fluido The study included both theoretical and experimental analisys. In the theoretical analisys a numerical mo deI based on control volume method is developed to simulate the lasing process by solving the energy equation in cilindrical coordinates. A data acquisition system based on virtual instruments concept is elaborated to analyse the heating process result,ing from Argon Laser radiation over an Aluminum foam porous medium immersed in four different fluids, namely air, water, polyalphaolefin oil and Mercury. An inverse methodology, estabilished by comparison of numerical and experimental results, is used to obtain the attenuation coeflicient of the saturated metal foam. Calculations also included the use of two models for the effective thermal conductivity. Results indicated the existence of a linear correlation between laser attenuation and a defined equivalent Prandtl number / Doutorado / Termica e Fluidos / Doutor em Engenharia Mecânica
539

Avaliação in vitro da condutibilidade hidráulica da dentina após a irradiação de diferentes parâmetros de laser associados ou não com verniz fluoretado / In vitro evaluation of dentin hydraulic conductance after irradiation with different LASER parameters, associated or not with a fluoride varnish

Rizzante, Fabio Antonio Piola 24 February 2015 (has links)
Os tratamentos que visam a remissão dos sintomas provocados pela hipersensibilidade dentinária são baseados na obliteração física dos túbulos dentinários com o objetivo de reduzir a condutibilidade hidráulica da dentina. Nesse contexto, diversos tratamentos têm sido avaliados, entre eles, a irradiação da superfície dentinária com laser. O objetivo do presente estudo foi avaliar a condutibilidade hidráulica da dentina radicular bovina, após irradiação com um laser de diodo (980nm), associado ou não com um verniz fluoretado. Sessenta espécimes de dentina radicular bovina com dimensões 5,5 X 5,5 X 1mm foram divididos em 6 grupos (n=10): Grupos 1, 3 e 5 (laser de diodo com 0,5; 0,7 e 1W respectivamente); Grupos 2, 4 e 6 (associação entre verniz fluoretado e laser de diodo com 0,5; 0,7 e 1W). A condutibilidade hidráulica da dentina, avaliada em FLODEC, foi analisada em 4 momentos: após criação da smear layer (permeabilidade mínima), após condicionamento com gel de ácido fosfórico a 37% (permeabilidade máxima), após os tratamentos propostos e após o desafio ácido com ácido cítrico a 6%. Os dados obtidos foram avaliados pela ANOVA a 2 critérios e teste de Tukey para comparação entre os diferentes grupos e ANOVA 1 critério de medidas repetidas e teste de Tukey para comparação entre as diferentes leituras dentro de cada grupo, sempre com p<0,05%. As análises demonstraram um melhor resultado conforme o aumento da potência de irradiação, principalmente se esta for associada à aplicação do verniz fluoretado, garantindo uma melhor estabilidade dos tratamentos, mesmo após o desafio ácido. Após essa última etapa, os espécimes foram avaliados quanto à composição mineral em EDX, apresentando como principais alterações nos grupos irradiados com laser, um aumento na proporção de ions Cálcio e Fósforo, associada à redução dos íons Carbono e Oxigênio. Dessa forma, a irradiação da dentina exposta com laser de diodo promoveu redução significante na condutibilidade hidráulica, principalmente com densidades de energia mais elevadas e quando associado ao verniz fluoretado. / The dentin hypersensitivity treatments are based on the physical obliteration of dentinal tubules in order to reduce the dentin hydraulic conductance. Hence, many treatments have been evaluated, such as the dentin surface laser irradiation. The aim of the present study was to evaluate the bovine root dentin hydraulic conductance after 980nm diode laser irradiation, associated or not with a fluoride varnish. Sixty bovine root dentin specimens were divided into six groups (n=10): Groups 1, 3 and 5 (0.5, 0.7 and 1W diode laser respectively); Groups 2, 4 and 6 (association between fluoride varnish application and 0.5, 0.7 and 1W diode laser irradiation). The dentin hydraulic conductance was evaluated at 4 moments, with FLODEC: after smear layer (minimum permeability), after 37% phosphoric acid gel etching (maximum permeability), after the treatments and after 6% citric acid challenge. The analysis demonstrated a better result with increased irradiation power, especially if the diode laser irradiation was associated with the application of a fluoride varnish, ensuring a greater treatment stability, even after acid challenge. After the citric acid challenge, the specimens were evaluated for mineral composition in EDX. The irradiated groups showed, as major changes after laser irradiation, an increase in the proportion of calcium and phosphorus ions, associated with the reduction of carbon and oxygen ions. In conclusion, the laser irradiation of the exposed dentin promoted a significant reduction in hydraulic conductance, mainly with higher energy densities and when combined with a fluoride varnish.
540

Influência de aplicações do "laser" érbio:YAG sobre a viabilidade microbiana, sua resistência a drogas e atividade hemolítica /

Lopes, Angélica Marquezim. January 2003 (has links)
Resumo: A atividade antimicrobiana do laser Er:YAG foi avaliada sobre biofilme bacteriano constituído por Escherichia coli ATCC 8739, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538 e 3 cepas de Fusobacterium nucleatum e sobre biofilme de microrganismos salivares. Os biofilmes foram submetidos à ação do laser a 1,2 W e 10 Hz por 5, 10, 15, 20, 30 e 60 s e fez-se a avaliação da microbiota residual em ágar sangue, em anaerobiose. O biofilme salivar se mostrou mais sensível nos primeiros tempos de irradiação. A redução microbiana em relação ao controle foi estatisticamente significativa entre todos os tempos testados. Avaliou-se também a ação do laser Er:YAG sobre 7 cepas de Fusobacterium nucleatum inoculadas sobre a superfície de corpos-de-prova (5mmX4mm) de dentes extraídos. Fez-se a aplicação do laser nos mesmos parâmetros físicos mencionados anteriormente, durante 15 s, levando à eliminação total do conteúdo séptico. O estudo avaliou também a irradiação do laser de Er:YAG durante tempos subinibitórios sobre a atividade hemolítica e susceptibilidade de 9 cepas de Fusobacterium nucleatum a amoxicilina, eritromicina, metronidazol e tetraciclina. Após a irradiação do laser, determinou-se a concentração inibitória mínima (CIM) para as drogas através do método de diluição em ágar. A ação do laser sobre a atividade hemolítica foi determinada em sangue humano. Verificou-se que o laser Er:YAG não afetou a atividade hemolítica de Fusobacterium nucleatum, que se mostrou α-hemolítica, tampouco a susceptibilidade a drogas dos isolados testados. / Abstract: Antimicrobial activity of Er:YAG laser was evaluated on a bacterial biofilm constituted by Escherichia coli ATCC 8739, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538 and 3 strains of Fusobacterium nucleatum and on biofilm produced by salivary microorganisms. Biofilms were irradiated by Er:YAG laser, 1,2 W and 10 Hz, for 5, 10, 15, 20, 30, and 60 s and the evaluation of residual contamination was performed on blood agar, under anaerobiosis. It was verified that salivary biofilm showed to be more susceptibility to the Er:YAG laser in shorter periods of laser irradiation. Bacterial reduction was significative in all tested periods of irradiation. The activity of Er:YAG laser was also evaluated on 7 strains of Fusobacterium nucleatum inoculated on samples of human dentin (5mm X 4mm), obtained from extracted teeth. The laser was used following the same physical parameters, as previously described, for 15 s, leading to complete elimination of their septic content. The study also evaluated the effects of subinibitory irradiation of Er:YAG laser on bacterial susceptibility of 9 strains of Fusobacterium nucleatum to antimicrobial drugs (amoxicillin, erythromycin, metronidazole, tetracycline) and hemolysis. Thus, after laser irradiation, the minimal inhibitory concentration of antimicrobial drugs was determined by using an agar dilution method. The influence of laser on hemolysis was carried out on human blood. It was verified that Er:YAG laser did not produce any measurable effect on hemolytic activity and the microbial susceptibility to tested antimicrobial drugs. / Orientador: José Ricardo Kina / Coorientador: Elerson Gaetti Jardim Júnior / Mestre

Page generated in 0.055 seconds