Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:44Z No. of bitstreams: 5 saulo.pdf: 2174121 bytes, checksum: 837d6359c95ce3c616f0b0f600ff2489 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T10:50:10Z (GMT) No. of bitstreams: 5 saulo.pdf: 2174121 bytes, checksum: 837d6359c95ce3c616f0b0f600ff2489 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T10:50:10Z (GMT). No. of bitstreams: 5 saulo.pdf: 2174121 bytes, checksum: 837d6359c95ce3c616f0b0f600ff2489 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / Muito ainda se discute com rela??o ? fisiopatologia das les?es perif?ricas de c?lulas gigantes (LPCG) e les?es centrais de c?lulas gigantes (LCCG). Ambas as les?es apresentam caracter?sticas cl?nicas distintas, apesar de possu?rem caracter?sticas histol?gicas semelhantes. Assim, estudos imunoistoqu?micos em LPCG e LCCG est?o sendo realizados, para permitir um melhor entendimento dessas les?es. Tem sido relatado que a express?o aumentada de FASN e a angiog?nese est?o diretamente ligadas com desenvolvimento dos tumores. No entanto, ainda n?o se sabe se estes eventos est?o envolvidos na patog?nese das LPCG e LCCG. O objetivo deste trabalho foi avaliar a express?o de FASN e o grau de angiog?nese entre LPCG e LCCG, al?m de verificar a correla??o entre essas vari?veis. Assim, 13 casos de LCCG e 14 casos de LPCG foram selecionados para an?lise da express?o imunoistoqu?mica de FASN, CD34, CD105 e D2-40. A express?o de FASN foi avaliada nos componentes celulares da les?o, seguida da mensura??o da densidade microvascular (DMV) e ?rea microvascular (AMV) para cada uma das amostras selecionadas. Os dados coletados foram submetidos ? an?lise descritiva e sequencialmente aos testes de Mann Whitney, teste t para amostras independentes e testes de correla??o de Pearson e Spearman. Os resultados do nosso estudo indicam que: (1) n?o h? diferen?as na imunoexpress?o de FASN entre os grupos de les?es (CM ? 8% FASN+ / CGM ? 38% FASN+); (2) LPCG possuem maior DMV em CD34; n?o houve diferen?as na DMV em CD105 e D2-40 entre as les?es. A AMV em LPCG foi maior que em LCCG para CD34, CD105 e D2-40; (3) em LPCG houve correla??o positiva entre (CM ? FASN+ com DMV/CD105); (4) nas LCCG houve correla??o positiva entre (CM ? FASN+ com DMV/CD105), (CM ? FASN+ com AMV/CD105 e CD34), (CGM ? FASN+ com AMV/CD105). A partir dos resultados obtidos concluiu-se que os n?veis similares da express?o imunoistoqu?mica de FASN indicam processos constitutivos da manuten??o tissular de ambas as les?es. No entanto, as diferen?as na vasculariza??o, entre os grupos de les?es, parecem ser influenciadas por CM positivas para FASN. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT There is still a lot of discussion about the pathophysiology of Peripheral Giant Cell Lesion (PGCL) and Central Giant Cell Lesion (CGCL). These lesions show distinct clinical features, although they have similar histological characteristics. Thus, immunohistochemical studies in PGCL and CGCL are being done to improve understanding these diseases. It has been reported that high level of FASN and angiogenesis are linked with tumors development. However, remains unknown whether these events are involved in the pathogenesis of LPCG and LCCG. The aim of this research was to study FASN expression and angiogenesis degree between PGCL and CGCL, in addition, verify the correlation between this variables. Thus, 13 cases of CGCL and 14 cases of PGCL were selected and examined by immunoexpression of FASN, CD34, CD105 and D2-40. The immunoexpression of FASN was assessed in components cells of lesions, followed by measurement of Microvassel Density (MVD) and Microvassel Area (MVA) for each selected sample. Data collected was submitted to descriptive analysis and followed by Mann Whitney test, ?t? test to independent samples and Person?s and Spearman?s correlation. The results of this study indicate that: (1) there are no differences in FASN immunoexpression between group lesions (MC ? 8% FASN+ / MGC ? 38% FASN+); (2) PGCL have greater MVD in CD34 than CGCL; there are no MDV differences in CD105 and D2-40 between lesions. PGCL have greater MVA in CD34, CD105 and D2-40 than CGCL; (3) in PCGL there was a positive correlation between (MC ? FASN+ and MVD/CD105); (4) in CGCL there was a positive correlation between (MC ? FASN+ and MVD/CD105), (MC ? FASN+ and MVA/CD105 and CD34), (MGC ? FASN+ and MVA/CD105). With base on these results it is concluded that similar expression of FANS levels indicate constituent process of tissue maintenance in both lesions. On the other hand, differences on angiogenesis between lesions seem be influenced by FASN+ mononuclear cells.