Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:43Z No. of bitstreams: 5 rafaela.pdf: 1470955 bytes, checksum: eed92eb403aa475a00a1b8d776f95ebc (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T10:51:15Z (GMT) No. of bitstreams: 5 rafaela.pdf: 1470955 bytes, checksum: eed92eb403aa475a00a1b8d776f95ebc (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T10:51:15Z (GMT). No. of bitstreams: 5 rafaela.pdf: 1470955 bytes, checksum: eed92eb403aa475a00a1b8d776f95ebc (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2014 / O objetivo do presente estudo foi verificar se a aplica??o t?pica de bebidas alco?licas destiladas, cacha?a e u?sque, altera a espessura epitelial da mucosa lingual de hamsters. Ap?s c?lculo amostral, 720 campos microsc?picos foram utilizados para mensura??o da espessura total do epit?lio de l?ngua (camada epitelial mais camada c?rnea). Quarenta hamsters foram divididos em dois grupos experimentais, conforme aplica??o da subst?ncia teste, em grupo 1 (cacha?a 48?gl) e grupo 2 (u?sque 40?gl), ambos avaliados em per?odos experimentais de 13 e 20 semanas. A borda contralateral da l?ngua, que n?o recebeu a subst?ncia teste, foi utilizada como controle. Ap?s eutan?sia, as l?nguas foram dissecadas, fixadas em solu??o de formol 10% e processadas para a obten??o de cortes histol?gicos, que foram corados pela t?cnica de hematoxilina e eosina (HE). Para a an?lise morfom?trica, tr?s l?minas por esp?cime foram avaliadas. A espessura do epit?lio, de ambas as bordas da l?ngua, foi mensurada com aux?lio do Programa Motic Images Plus 2.0 (Motic?). Os dados da an?lise morfom?trica foram avaliados pela estat?stica descritiva e teste de Wilcoxon e teste de Mann-Whitney. No grupo cacha?a, em rela??o ? espessura total do epit?lio, houve diferen?a estat?stica significativa entre a m?dia do lado teste, 61,87 (14,75)?m e a m?dia do lado controle, de 67,20 (9,96)?m (p=0,044). Tamb?m observou-se uma redu??o altamente significativa da espessura das camadas epitelial (p<0,001) e c?rnea (p=0,021). No per?odo de 13 semanas, houve diminui??o estat?stica significativa da espessura das camadas c?rnea (p=0,032) e epitelial (p<0,001), enquanto que, em 20 semanas, somente a camada epitelial apresentou-se significativamente reduzida (p=0,002). No grupo u?sque houve aumento significativo da espessura da camada c?rnea (p=0,015) e epitelial (p=0,012) em 13 semanas. Conclui-se que tipos diferentes de bebidas alco?licas destiladas, com concentra??o alco?lica entre 40?gl e 48?gl, promoveram altera??es morfom?tricas e morfol?gicas diferenciadas. A cacha?a promoveu atrofia epitelial, o que poderia facilitar a penetra??o de sust?ncias ou agentes carcinog?nicos. J? o u?sque promoveu hiperplasia, podendo assim sugerir o in?cio do desenvolvimento de les?es pr?-malignas. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT The aim of this study was to determine whether topical application of distilled spirits, cacha?a and whiskey, alter the epithelial thickness of tongue mucosa of hamsters. After sample size calculation, 720 microscopic fields were used to measure the total thickness of the tongue epithelium (epithelial layer over the stratum corneum). Forty hamsters were divided into two groups, depending on the application of the test substance in group 1 (cacha?a 48?gl) and group 2 (whiskey 40?gl), both evaluated in the experimental periods of 13 and 20 weeks. The contralateral side of the tongue, which has not received the test substance was used as control. After euthanasia, the tongues were dissected, fixed in 10% formalin solution, processed to obtain histological sections and were stained by hematoxylin and eosin (HE). For the morphometric analysis, three slides per specimen were evaluated. The thickness of the epithelium, both edges of the tongue, was measured with the aid of the program Motic Images Plus 2.0 (Motic ?). Data from morphometric analysis were evaluated by descriptive statistics and by Wilcoxon and Mann-Whitney tests. In cacha?a group, regarding the total thickness of the epithelium, there was a statistically significant difference between the average of the test side, 61.87 (14.75) ?m and the average of the control side, 67.20 (9.96) ?m (p=0.044). It was also observed a highly significant reduction in the thickness of the epithelium (p<0.001) and the cornea (p=0.021). At 13 weeks, there was a statistically significant decrease in the thickness of the cornea (p=0.032) and epithelial (p<0.001), whereas at 20 weeks, only the epithelial layer was significantly reduced (p=0.002). In the Whiskey group, there was a significant increase in thickness of the cornea (p=0.015) and epithelial (p=0.012) at 13 weeks. It was conclude that different types of distilled spirits with alcohol concentration between 40 ?gl ?and 48 ?gl, promoted ?morphological and morphometric changes differentiated. Cacha?a promoted epithelial atrophy, which could facilitate the penetration of substances give or carcinogens. The whiskey promoted hyperplasia which suggest the early development of premalignant lesions.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:43Z No. of bitstreams: 5 anne.pdf: 708252 bytes, checksum: 4181732fe1d95bea52fbf78610b24a96 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T11:08:27Z (GMT) No. of bitstreams: 5 anne.pdf: 708252 bytes, checksum: 4181732fe1d95bea52fbf78610b24a96 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T11:08:27Z (GMT). No. of bitstreams: 5 anne.pdf: 708252 bytes, checksum: 4181732fe1d95bea52fbf78610b24a96 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / A pesquisa intitulada ?Sa?de Bucal de portadores de HIV-AIDS: influ?ncia de fatores sist?micos e locais no determinante sa?de-doen?a?, foi realizada durante o ano de 2012 em Diamantina, Minas Gerais. O objetivo desta pesquisa foi avaliar as condi??es bucais e sist?micas de portadores HIV/AIDS atendidos pelo Programa Municipal de DST-AIDS de Diamantina, provenientes de 24 munic?pios do Vale do Jequitinhonha. Para isso, foram avaliados 118 pacientes com HIV-AIDS. Foram observadas as condi??es de sa?de bucal e geral dos pacientes, condi??es socioecon?micas, aspectos epidemiol?gicos da infec??o pelo HIV (forma de contamina??o e de diagn?stico), uso de terapia antirretroviral de alta pot?ncia (HAART), contagem de linf?citos T CD4, h?bitos presentes (fumo, ?lcool e outras drogas), bem como o acesso destes pacientes a servi?os de Odontologia. Os resultados revelaram que a maioria dos pacientes pertencia ao g?nero masculino (53,4%), a m?dia de idade encontrada foi de 39,7 anos, a forma de contamina??o de 98,3% dos pacientes foi por via sexual e a renda familiar de 44,9% dos pacientes foi de at? 1 (um) sal?rio m?nimo mensal. Foi observado um alto ?ndice de doen?as e les?es bucais, sendo as mais prevalentes as doen?as periodontais (70,3%), a hiperpigmenta??o da mucosa (52,5%), a queilite angular (32,2%) e ulcera??es aftosas recorrentes (29,7%). A hiperpigmenta??o de mucosa parece ser mais frequente entre pacientes em uso de HAART e em tabagistas. A candidose bucal foi associada com diabetes, anemia, xerostomia, uso de HAART e taxa de CD4 > 500 cel/mm3, enquanto a ulcera??o aftosa recorrente foi associada com tabagismo, etilismo e xerostomia. / ABSTRACT The research entitled "Oral Health of HIV-AIDS patients: influence of local and systemic factors" was held during the year 2012 in Diamantina, Minas Gerais. The aim of this research was to evaluate the oral and systemic conditions of patients with HIV / AIDS attended by Municipal DST-AIDS of Diamantina, from 24 cities of Jequitinhonha Valley. To do this, 118 patients with HIV-AIDS were evaluated. The patients were observed about their conditions of oral and general health, socioeconomic aspects, epidemiological aspects of HIV infection (form of contamination and diagnosis), use of highly active antiretroviral therapy (HAART), CD4 count, customs(smoking, alcohol and other drugs), as well as access of these patients to dental services. The results revealed that most of the patients were males (53.4%), mean age was 39.7 years, the form of contamination of 98.3% of patients was through sex and the family income of 44.9% of patients is a monthly wage like $362,00. There was a high rate of disease and oral lesions, the most prevalent periodontal diseases (70.3%), mucosal hyperpigmentation (52.5%), angular cheilitis (32.2%) and recurrent aphthous ulcerations ( 29.7%). Hyperpigmentation of mucosa seems to be more frequent among patients on HAART and in smokers. Oral candidiasis was associated with diabetes, anemia, xerostomia, HAART use and CD4 count> 500 cells/mm3, while recurrent aphthous ulceration was associated with smoking, alcohol and xerostomia.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:44Z No. of bitstreams: 5 saulo.pdf: 2174121 bytes, checksum: 837d6359c95ce3c616f0b0f600ff2489 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T10:50:10Z (GMT) No. of bitstreams: 5 saulo.pdf: 2174121 bytes, checksum: 837d6359c95ce3c616f0b0f600ff2489 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T10:50:10Z (GMT). No. of bitstreams: 5 saulo.pdf: 2174121 bytes, checksum: 837d6359c95ce3c616f0b0f600ff2489 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / Muito ainda se discute com rela??o ? fisiopatologia das les?es perif?ricas de c?lulas gigantes (LPCG) e les?es centrais de c?lulas gigantes (LCCG). Ambas as les?es apresentam caracter?sticas cl?nicas distintas, apesar de possu?rem caracter?sticas histol?gicas semelhantes. Assim, estudos imunoistoqu?micos em LPCG e LCCG est?o sendo realizados, para permitir um melhor entendimento dessas les?es. Tem sido relatado que a express?o aumentada de FASN e a angiog?nese est?o diretamente ligadas com desenvolvimento dos tumores. No entanto, ainda n?o se sabe se estes eventos est?o envolvidos na patog?nese das LPCG e LCCG. O objetivo deste trabalho foi avaliar a express?o de FASN e o grau de angiog?nese entre LPCG e LCCG, al?m de verificar a correla??o entre essas vari?veis. Assim, 13 casos de LCCG e 14 casos de LPCG foram selecionados para an?lise da express?o imunoistoqu?mica de FASN, CD34, CD105 e D2-40. A express?o de FASN foi avaliada nos componentes celulares da les?o, seguida da mensura??o da densidade microvascular (DMV) e ?rea microvascular (AMV) para cada uma das amostras selecionadas. Os dados coletados foram submetidos ? an?lise descritiva e sequencialmente aos testes de Mann Whitney, teste t para amostras independentes e testes de correla??o de Pearson e Spearman. Os resultados do nosso estudo indicam que: (1) n?o h? diferen?as na imunoexpress?o de FASN entre os grupos de les?es (CM ? 8% FASN+ / CGM ? 38% FASN+); (2) LPCG possuem maior DMV em CD34; n?o houve diferen?as na DMV em CD105 e D2-40 entre as les?es. A AMV em LPCG foi maior que em LCCG para CD34, CD105 e D2-40; (3) em LPCG houve correla??o positiva entre (CM ? FASN+ com DMV/CD105); (4) nas LCCG houve correla??o positiva entre (CM ? FASN+ com DMV/CD105), (CM ? FASN+ com AMV/CD105 e CD34), (CGM ? FASN+ com AMV/CD105). A partir dos resultados obtidos concluiu-se que os n?veis similares da express?o imunoistoqu?mica de FASN indicam processos constitutivos da manuten??o tissular de ambas as les?es. No entanto, as diferen?as na vasculariza??o, entre os grupos de les?es, parecem ser influenciadas por CM positivas para FASN. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT There is still a lot of discussion about the pathophysiology of Peripheral Giant Cell Lesion (PGCL) and Central Giant Cell Lesion (CGCL). These lesions show distinct clinical features, although they have similar histological characteristics. Thus, immunohistochemical studies in PGCL and CGCL are being done to improve understanding these diseases. It has been reported that high level of FASN and angiogenesis are linked with tumors development. However, remains unknown whether these events are involved in the pathogenesis of LPCG and LCCG. The aim of this research was to study FASN expression and angiogenesis degree between PGCL and CGCL, in addition, verify the correlation between this variables. Thus, 13 cases of CGCL and 14 cases of PGCL were selected and examined by immunoexpression of FASN, CD34, CD105 and D2-40. The immunoexpression of FASN was assessed in components cells of lesions, followed by measurement of Microvassel Density (MVD) and Microvassel Area (MVA) for each selected sample. Data collected was submitted to descriptive analysis and followed by Mann Whitney test, ?t? test to independent samples and Person?s and Spearman?s correlation. The results of this study indicate that: (1) there are no differences in FASN immunoexpression between group lesions (MC ? 8% FASN+ / MGC ? 38% FASN+); (2) PGCL have greater MVD in CD34 than CGCL; there are no MDV differences in CD105 and D2-40 between lesions. PGCL have greater MVA in CD34, CD105 and D2-40 than CGCL; (3) in PCGL there was a positive correlation between (MC ? FASN+ and MVD/CD105); (4) in CGCL there was a positive correlation between (MC ? FASN+ and MVD/CD105), (MC ? FASN+ and MVA/CD105 and CD34), (MGC ? FASN+ and MVA/CD105). With base on these results it is concluded that similar expression of FANS levels indicate constituent process of tissue maintenance in both lesions. On the other hand, differences on angiogenesis between lesions seem be influenced by FASN+ mononuclear cells.