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Syfte: Att ta reda på hur lång livslängd en bildplatta har avseende slitage och bildkvalité.Material och metod: Tre fabriksnya bildplattor användes för att undersöka hur en enskild faktor i arbetsgången för bildgenerering påverkar bildkvalitén. Den första plattan exponerades och skannades 300 gånger. Platta nummer två användes för att undersöka effekten av totalt 300 av- och påtagningar i en frigolithållare, och platta tre användes för motsvarande test av en plasthållare. Exponering skedde utan patient och mot en neutral bakgrund. För samtliga plattor togs i initialskedet en referensbild, och resulterande bilder från testerna granskades i Planmeca Romexis 3.8 och bedömdes enligt en skala 0-3. Plattorna i sig granskades även okulärt, och test av uniformitet gjordes av referensbild och bild 300 i serie 1 med hjälp av ImageJ. Resultat: Samtliga bilder från samtliga plattor bedömdes vara likvärdiga med referensbild. Vid okulär granskning var reporna klart mer uttalade hos den platta som skannats 300 gånger, men reporna återfanns endast på den sida som ej är avsedd att exponeras. Test av uniformitet fann ingen skillnad mellan referensbild och bild nr 300 i serie 1. Slutsats: Studien visar att varken röntgenexponering, skanning eller av- och påtagning i hållare av två olika slag har någon inverkan på bildkvalité efter 300 upprepningar då momenten gjordes var för sig. Vi konkluderar att plattan sannolikt är som mest sårbar då den placeras intraoralt, och föreslår att alla moment gjorda i följd delvis kan svara för den reduktion av bildkvalité som observerats i andra studier. / Aim: To determine the longevity of photostimulable storage phosphor plates (PSPs) with respect to wear and image quality. Method: Three brand new PSPs were used to assess how a solitary factor in the picture generation process affects the picture quality. The first plate was exposed and scanned 300 times. Plate number two was used to assess the effect of mounting and demounting the plate 300 times onto a holder made of styrofoam, and the third plate was used for a corresponding test with a plastic holder. A flat surface was exposed and no patients were involved. A reference picture was taken initially for each plate, and the resulting pictures from the tests were viewed using Planmeca Romexis 3.8 and were evaluated on a scale from 0-3. The individual plates also underwent ocular inspection, and a uniformity test was performed on the first and last picture taken with plate 1, using ImageJ. Results: Each picture from each plate was deemed equivalent to the reference picture. The ocular inspection revealed a more distinct band of scratches on the plate that was scanned the most, although the scratches were only found on the side not meant to receive exposure. No difference in uniformity was found between the first and last picture taken using plate 1. Conclusion: X-ray exposure, scanning and mounting and demounting of PSPs onto holders of two different kinds do not affect the picture quality after 300 repetitions when done separately. We conclude that the plate is likely most susceptible to wear when placed intraorally, and suggest that all the steps made in succession can partly account for the reduction in picture quality observed in other studies.

<p>Aging involves the progressive decline of physical performance and effective metabolic regulation. To date, dietary interventions to slow this deterioration have shown limited success. I tested the effectiveness of a complex dietary supplement (that targets five key mechanisms of aging) for ameliorating age-related declines in physical activity, metabolism and energetic efficiency in mice. Supplemented mice maintained youthful levels of daily physical activity in old age, compared with a progressive decline in untreated controls. The diet also influenced aspects of metabolic rate, as supplemented mice showed age-related increases in fasting oxygen consumption and respiratory quotient compared to declines in these biomarkers in untreated mice. Furthermore, oxygen consumption over 24-h was significantly lower in supplemented mice in spite of being more active than untreated mice. Taken in conjunction with higher resting respiratory exchange ratios across age, this suggests that supplemented mice may utilize more carbohydrate than lipid as an energy substrate and they may express increased metabolic efficiency. These results hold promise for augmenting youthful athleticism and extending geriatric functionality. I also assessed the impact of the supplement on age- related changes in biomarkers of oxidative stress in heart and kidney samples from normal (Nr) and transgenic (Tg) mice that over-express growth hormone. Measures of whole-tissue H2O2 in the heart showed no significant changes in Nr or Tg mice, but catalase activity was ~33% higher in supplemented Nr and Tg compared to untreated controls. Kidney tissue from Nr mice showed significant and opposite age-related trends of H2O2, increasing in supplemented mice and decreasing in untreated controls, however, no changes were observed in Tg mice. Catalase activity in kidney tissue remained unchanged in both genotypes regardless of diet. Furthermore, the ratio of reduced to oxidized glutathione was 43% higher in urine from older (>12 months-old) supplemented mice, indicative of substantially lower whole-body oxidative status. Lastly, older supplemented mice showed improved whole-body glucose tolerance compared with untreated counterparts. These results confirm that the supplement reduces aspects oxidative stress and improves insulin sensitivity, two of the key design criteria for formulating the supplement. This work represents proof of principle that complex dietary supplements can extend functional capacities associated with metabolism and energetic efficiency into older ages.</p> / Master of Science (MSc)

Financial services regulators are tasked with balancing the conflicting roles of empowering and policing their regulated communities. In order to be effective, agencies must be able to accomplish both tasks. This analysis examines several determinants of effectiveness among U.S. bank regulators. Using statistical and narrative analyses, it examines factors that have contributed to the regulatory effectiveness of the National Credit Union Administration, Office of the Comptroller of the Currency, and Office of Thrift Supervision. The study focused on the relationships between regulatory ability to prevent failures and influences including agency longevity, ability to manage complexity, appointee and staff qualities, mission stability, regulatory style, and resources. Agency longevity and resources had the greatest impact on effectiveness among the cases that were examined. Additionally, this study proposes a typology that suggests that more effective regulators are able to balance information from their regulated communities with a public interest orientation. This allows them to have current information regarding emerging regulatory issues but also to avoid becoming too reliant on their supervised institutions for information. / Ph. D.

The effects of temperature on emergence, development, oviposition, longevity and fecundity of <i>Cotesia orobenae</i> Forbes (Hymenoptera: Braconidae) were studied. Development time of parasitoid pupae decreased as temperature increased to 35°C. Temperatures between 20 and 25°C were most suitable for parasitoid development, oviposition and fecundity. Longevity was significantly affected by temperature, and was greatest between I5 and 20°C for both sexes. Sex ratios obtained from virgin females, females mated once and females exposed to multiple mating were compared. Virgin females produced only males, those with single or multiple mating produced more females than males. <i>Cotesia orobenae</i> exposed to five densities of third instar cross-striped cabbageworm, <i>Evergestis rimosaiis</i> (Guenee) (Lepidoptera: Pyralidae), for 24 h showed differences in percent parasitization among the treatments. The highest number of parasitoid cocoon masses was obtained from a density of 10 hosts. / Ph. D.

Selection strategies to increase productive traits of farm animals have been effective and highly specialized breeds and strains were obtained. At the same time, the effort made to obtain extremely high producing animals was accompanied by undesired effects, such as a reduced ability to sustain production, reproduction and health; especially under constrained conditions. The perception that selection was degrading robustness, lead to selection strategies aiming to improve the ability of animals to perform in a wider range of environmental constrain. However, at the present moment, the physiological mechanisms allowing farm animals to perform well in a wide range of environments, while others succumb, have not been described. The present thesis intended to address this question by describing the evolution of traits related to fitness, survival and to the adaptability to environmental constraints. Two maternal rabbit lines differing in their ability to face the environmental constraints, i.e. a `specialist¿ and a `generalist¿ maternal rabbit line were available. Additionally, two generations (20 generations apart) of the specialized line were simultaneously available. During the first two consecutive reproductive cycles, female rabbits were simultaneously subjected to three environmental conditions differing in the intensity and in the physiological constrain imposed. Digestive capacity, the acquisition of resources and the partitioning of resources into different function (i.e. litter size, milk yield, growth, body reserves, etc.) was also assessed. Results showed a greater acquisition capacity of `generalist¿ females in constrained conditions with respect to `specialist¿ females. Moreover, the greater acquisition capacity was not accompanied by a reduction in the digestive efficiency, allowing the `generalist¿ females a relative greater acquisition of digestible energy. The maintenance of reproductive performance by having a greater acquisition capacity, together with the avoidance of making an intensive use of body reserves were both related to the capacity of `generalist¿ females to sustain reproduction in a wide range of environmental conditions. Twenty generations of selection exclusively for reproduction (specialized line), was not accompanied by a higher acquisition capacity, but by a change in the relative priority between the litter being nursed (actual) and the litter being gestate (future litter). In this sense, females from the actual generation of selection for litter size at weaning had a greater milk yield in the first week of lactation (period of great importance to kits survival), reducing it by the end of lactation. The present thesis also evidenced the importance of the environment where the animals are being selected in the evolution of the interplay between competing functions. / Savietto, D. (2014). Environmental and genetic factors driving robustness in reproductive rabbit does [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/37198 / Premios Extraordinarios de tesis doctorales

Xenohormesis suggests that consuming stressed food provides physiological adaptation to the consumer for similar stresses. To evaluate this, Daphnia magna was provided with heat-stressed Nannochloropsis limnetica grown at 25℃ whereas the control group was provided Nannochloropsis limnetica grown at 12℃. In the heat tolerance experiment, Daphnia kept at 28℃ and 20℃ while consuming 25℃ algae had significantly higher heat tolerance compared to the control. Additionally, in stressed conditions, Daphnia consuming 25℃-grown food had significantly higher lifespan compared to the control. Next, Daphnia consuming 25℃-grown algae had significantly higher levels of antioxidants which could explain the lifespan extension. Finally, in fatty acid analysis, eicosatetraenoic acid (EPA) was found lower in Daphnia consuming 25℃ cultures compared to Daphnia consuming 20℃ cultures. We speculate that this lower EPA level is increasing membrane rigidity and hence higher heat tolerance is observed. These findings indicate consumption of stressed algae might provide physiological benefits in Daphnia.

Nothing is perfect, and this includes the ability to maintain homeostasis within the cell with age. Factors such as aging, chemicals, and gene dysfunction disrupt cellular homeostasis, leading to increased stress and compromising the ability of animals to maintain a healthy lifespan. Dysregulated homeostasis can be detrimental on an organismal level, impacting locomotion, and on a cellular level causing proteins to misfold and become aggregates, which are toxic to cells. Toxic protein aggregation and loss of locomotory function are key hallmarks of several age-related diseases. My Ph.D. work examined the collapse of homeostasis on electrotaxis, the age-associated increase in proteotoxicity, the decline in longevity, and neuronal and muscle health. On a behavioural level I demonstrated that loss of various components of the MT-UPR, ER-UPR, and HSR modulated the speed of animals. Additionally, I found that activation of stress responses due to chemicals and exercise reduced and increased the speed of animals respectively. On a cellular level I elucidated potential mechanisms by which Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) affects the stress response to maintain homeostasis and prevent protein aggregation. I observed the novel localization and role of MANF in lysosomes to potentially act as a critical regulator of the stress response to maintain proteostasis, neuronal health and longevity, thereby bringing balance to the cell. Furthermore, the broad tissue expression of MANF revealed its localization to muscles. This supports the ability of MANF to act as more than a neurotrophic factor as it was found to be required for muscular health in animals in an age-dependent manner. Overall, my Ph.D. research has provided new insights into the stress response and behaviour and the precise role of MANF in mediating stress response signaling to promote organismal and cellular fitness. / Dissertation / Doctor of Science (PhD) / Cellular perturbations or stress disrupt homeostasis, activating multiple stress responses. Activation of the stress response can determine the fate of an organism and is crucial to its health. Although the stress response pathways are generally understood, little is known about how the stress responses preserve animal behaviour or how they are regulated to promote organismal survival. My work has provided a basis for how stress responses affect behaviour positively and negatively in animals. I found that the stress response required mesencephalic astrocyte derived neurotrophic factor (MANF) to promote organismal survival. My thesis determined that MANF acts as more than a neurotrophic factor. MANF was found to not only be essential in neuronal health but also longevity and muscle health. Overall, this thesis demonstrated the impact of stress response on behaviour and the potential mechanism by which MANF is cytoprotective in whole organisms.