O manejo populacional de cães e gatos é um conjunto de estratégias para controlar e prevenir problemas relacionados com o convívio entre esses animais e os seres humanos. Nesta tese é proposto um fluxo de trabalho baseado em métodos quantitativos, para auxiliar o planejamento, implementação e monitoramento de programas de manejo populacional de animais de companhia. Ao seguir o fluxo de trabalho é possível coletar dados para caracterizar populações, analisar esses dados para propor intervenções e avaliar o efeito das intervenções. A proposta foi baseada na articulação de cinco pesquisas. Na primeira pesquisa foi implementado de um desenho amostral complexo para caracterizar a população de cães e gatos domiciliados de Votorantim, São Paulo. Na segunda pesquisa que foi baseada nos dados levantados na primeira, foram usadas análises de correspondências múltiplas para identificar perfis de opiniões públicas em relação ao abandono de cães e gatos. Na terceira pesquisa foi avaliada a validade do desenho amostral usado na primeira pesquisa, mediante simulações estocásticas. Na quarta pesquisa foi desenvolvido um modelo matemático de manejo populacional que permite priorizar as intervenções de acordo com o efeito que produzem. Na quinta pesquisa foi desenvolvido um modelo matemático para avaliar a eficiência do controle reprodutivo realizado com contraceptivos de efeito reversível. Os modelos das duas últimas pesquisas foram baseados em sistemas acoplados de equações diferenciais e em análises de sensibilidade global e local. A proposta foi implementada em um software de código aberto, o pacote do R capm, que pode ser incorporado na rotina de trabalho dos setores envolvidos no manejo populacional de animais de companhia / Dog and cat population management is a set of strategies to control and prevent problems related with the coexistence between those animals and human beings. In this thesis it is proposed a work-fiow based on quantitative methods to support the planning, implementation and mo- nitoring of companion animal population management programs. Following the work-fiow, it is possible to collecf data to characterize populations, analyze that data to propose interventions and assess the effect of interventions. The proposal was based on the articulation of five rese- arches. In the first research, a complex sampling design was implemented to characterize the owned dog and cat populations of Votorantim, São Paulo. In the second research, which was based on data from the first one, public opinion profiles regarding dog and cat abandonment were identified using multiple correspondence analysis. In the third research, the validity of the sampling design used in the first research was assessed through stochastic simulations. In the fourth research, a mathematical model of population management was developed. With that model, it is possible to prioritize interventions according to the effect they produce. In the fifth research, a mathematical model was developed to assess the efficiency of reproductive control based on contraceptives with reversible effect. The models of the last two researches were based on systems of coupled differential equations, and on global and local sensitivity analysis. The proposal was implemented in an open source software, the R package capm, that can be incorporated in the working routine of sectors involved with companion animal population management

R-phenylacetylcarbinol (PAC) is used as a precursor for production of ephedrine and pseudoephedrine, which are anti-asthmatics and nasal decongestants. PAC is produced from benzaldehyde and pyruvate mediated by pyruvate decarboxylase (PDC). A strain of Rhizopus javanicus was evaluated for its production of PDC. The morphology of R. javanicus was influenced by the degree of aeration/agitation. A relatively high specific PDC activity (328 U decarboxylase g-1 mycelium) was achieved when aeration/agitation were reduced significantly in the latter stages of cultivation. The stability of partially purified PDC and crude extract from R. javanicus were evaluated by examining the enzyme deactivation kinetic in various conditions. R. javanicus PDC was less stable than Candida utilis PDC currently used in our group. A kinetic model for the deactivation of partially purified PDC extracted from C. utilis by benzaldehyde (0?00 mM) in 2.5 M MOPS buffer has been developed. An initial lag period prior to deactivation was found to occur, with first order dependencies of PDC deactivation on exposure time and on benzaldehyde concentration. A mathematical model for the enzymatic biotransformation of PAC and its associated by-products has been developed using a schematic method devised by King and Altman (1956) for deriving the rate equations. The rate equations for substrates, product and by-products have been derived from the patterns for yeast PDC and combined with a deactivation model for PDC from C. utilis. Initial rate and biotransformation studies were applied to refine and validate a mathematical model for PAC production. The rate of PAC formation was directly proportional to the enzyme activity level up to 5.0 U carboligase ml-1. Michaelis-Menten kinetics were determined for the effect of pyruvate concentration on the reaction rate. The effect of benzaldehyde on the rate of PAC production followed the sigmoidal shape of the Monod-Wyman-Changeux (MWC) model. The biotransformation model, which also included a term for PDC inactivation by benzaldehyde, was used to determine the overall rate constants for the formation of PAC, acetaldehyde and acetoin. Implementation of digital pH control for PAC production in a well-stirred organic-aqueous two-phase biotransformation system with 20 mM MOPS and 2.5 M dipropylene glycol (DPG) in aqueous phase resulted in similar level of PAC production [1.01 M (151 g l-1) in an organic phase and 115 mM (17.2 g l-1) in an aqueous phase after 47 h] to the system with a more expensive 2.5 M MOPS buffer.

An experimental and modelling investigation of a tetrafunctional initiator designed for free radical polymerizations is presented. Multifunctional initiators are believed to provide two advantages over traditional monofunctional initiators. With a higher number of functional sites per molecule, they are able to increase polymer production while simultaneously maintaining or increasing polymer molecular weight. Examination of the literature indicates the majority of academic and industrial published studies have investigated difunctional initiators with most focusing on styrene. In this thesis, a tetrafunctional initiator, JWEB50, was systematically investigated for a variety of monomer systems in order to develop a better understanding of the behaviour of multifunctional initiators in free radical polymerizations. <br /><br /> A kinetic study comparing the tetrafunctional initiator to a monofunctional counterpart, TBEC, demonstrated that the impact of a multifunctional initiator is dependent upon monomer type. Regardless of the homo- or copolymer system examined, it was observed that the tetrafunctional initiator could produce higher rates of polymerization due to the greater number of labile groups per initiator molecule. However, the influence of the tetrafunctional initiator on the polymer molecular weight was dictated by the polymerization characteristics of the system in question. In the case of styrene, the tetrafunctional initiator maintained similar molecular weights compared to the monofunctional initiator while for methyl methacrylate (MMA), switching from a mono- to a tetrafunctional initiator actually decreased the polymer molecular weight. Other monomers such as butyl acrylate and vinyl acetate and copolymers of MMA and styrene or alpha-methyl styrene were examined to study the effect of initiator functionality in free radical polymerizations. <br /><br /> Subsequent to the kinetic investigation, polystyrene and poly(methyl methacrylate) samples produced with the tetrafunctional initiator were characterized in detail in order to examine the effects of initiator functionality on polymer properties. Samples generated with the monofunctional initiator were used for comparison purposes. Chromatographic and dilute solution methods were able to detect significant levels of branching in the polystyrene sample produced with JWEB50, while poly(methyl methacrylate) samples showed no evidence of branching. Rheological tests involving a combination of oscillatory and creep shear measurements were completed in order to detect differences between samples. The presence of branching using rheological techniques was clearly observed for both polystyrene and poly(methyl methacrylate) samples produced with the tetrafunctional initiator. <br /><br /> In order to explain the experimental results observed in the kinetic and polymer properties studies, a reaction mechanism for polymerizations initiated with a tetrafunctional initiator was proposed and used in the development of a mathematical model. Reactions involving the fate/efficiency of functional groups are properly accounted for, while in the past this had been ignored by modelling work in the literature. Based on model predictions, di-radical concentrations were estimated to be several orders of magnitude smaller than mono-radical concentrations and their contribution in the reaction mechanism was found to be negligible. Modelling results also demonstrated that the concentration and chain length of various polymer structures (i. e. , linear, star or coupled stars) depend upon monomer type and reaction conditions.

An experimental and modelling investigation of a tetrafunctional initiator designed for free radical polymerizations is presented. Multifunctional initiators are believed to provide two advantages over traditional monofunctional initiators. With a higher number of functional sites per molecule, they are able to increase polymer production while simultaneously maintaining or increasing polymer molecular weight. Examination of the literature indicates the majority of academic and industrial published studies have investigated difunctional initiators with most focusing on styrene. In this thesis, a tetrafunctional initiator, JWEB50, was systematically investigated for a variety of monomer systems in order to develop a better understanding of the behaviour of multifunctional initiators in free radical polymerizations. <br /><br /> A kinetic study comparing the tetrafunctional initiator to a monofunctional counterpart, TBEC, demonstrated that the impact of a multifunctional initiator is dependent upon monomer type. Regardless of the homo- or copolymer system examined, it was observed that the tetrafunctional initiator could produce higher rates of polymerization due to the greater number of labile groups per initiator molecule. However, the influence of the tetrafunctional initiator on the polymer molecular weight was dictated by the polymerization characteristics of the system in question. In the case of styrene, the tetrafunctional initiator maintained similar molecular weights compared to the monofunctional initiator while for methyl methacrylate (MMA), switching from a mono- to a tetrafunctional initiator actually decreased the polymer molecular weight. Other monomers such as butyl acrylate and vinyl acetate and copolymers of MMA and styrene or alpha-methyl styrene were examined to study the effect of initiator functionality in free radical polymerizations. <br /><br /> Subsequent to the kinetic investigation, polystyrene and poly(methyl methacrylate) samples produced with the tetrafunctional initiator were characterized in detail in order to examine the effects of initiator functionality on polymer properties. Samples generated with the monofunctional initiator were used for comparison purposes. Chromatographic and dilute solution methods were able to detect significant levels of branching in the polystyrene sample produced with JWEB50, while poly(methyl methacrylate) samples showed no evidence of branching. Rheological tests involving a combination of oscillatory and creep shear measurements were completed in order to detect differences between samples. The presence of branching using rheological techniques was clearly observed for both polystyrene and poly(methyl methacrylate) samples produced with the tetrafunctional initiator. <br /><br /> In order to explain the experimental results observed in the kinetic and polymer properties studies, a reaction mechanism for polymerizations initiated with a tetrafunctional initiator was proposed and used in the development of a mathematical model. Reactions involving the fate/efficiency of functional groups are properly accounted for, while in the past this had been ignored by modelling work in the literature. Based on model predictions, di-radical concentrations were estimated to be several orders of magnitude smaller than mono-radical concentrations and their contribution in the reaction mechanism was found to be negligible. Modelling results also demonstrated that the concentration and chain length of various polymer structures (i. e. , linear, star or coupled stars) depend upon monomer type and reaction conditions.

This thesis presents the analysis of complex slope deformations through the application of numerical modelling techniques. Complex slope deformations, in this thesis, include cases where the use of more conventional analytical tools such as limit equilibrium techniques or the use of empirical criteria are not readily applicable. Such a scenario often results from adverse geological and environmental conditions or from human activity. Examples of complex slope deformations are the influence of underground mining on a slope, or situations where rigid jointed rocks overly relatively weak layers. The use of numerical modelling techniques, both continuum and discontinuum, in the analysis of slope stability problems has increased rapidly in the last decade and proved valuable in the analysis of complex geomechanical problems. Two numerical modeling programs FLAC (Fast Lagrangian Analysis of Continua) and UDEC (Universal Distinct Element Code) were used in this thesis. Three main groups of problems were investigated: (1) The analysis of deformation associated with rigid jointed rocks overlying relatively weak layers including a case study involving deformation taking place in the foundation of the Spis Castle in Slovakia. It was demonstrated that the type of deformation in such cases depends on the strength, deformability and thickness of the weak layer as well as the jointing pattern of the overlying rocks. It was shown, that the deformations at Spis castle are governed primarily by the presence of a weak, plastic "creep zone" under the base of the travertine blocks on which the castle is founded. (2) The analysis of toppling deformation in a weak rock slope comprising several lithostratigraphic units at the Luscar Mine, Alberta. It was found that the instability mechanism in the initial phase was flexural toppling, confined to a distinct quasi-linear failure surface which provided the shear plane for subsequent sliding movement. A prediction of slope stability for a planned mine extension in the same pit was made, thereby determining "safe excavation limits". (3) The analysis of interaction between underground mining and slope instability. The analyses of various slope deformation mechanisms that can be induced by underground mining are presented. The analysis of the Frank Slide in southwestern Alberta illustrated the critical role of underground mining at the base of the Turtle Mountain on triggering the final slope failure. The analyses present within this thesis demonstrate the application of numerical modelling techniques in the characterization of complex slope deformations. New interpretations of existing failure mechanisms were presented in the case of the Frank Slide, and improved understanding of the failure mechanism and slope deformation were gained in the Luscar Mine and Spis Castle case studies. Furthermore, hypothetical modelling studies relevant to underground mining and block-type deformations allow an increased understanding of complex slope deformations.

Προσομοίωση της υδάτινης κυκλοφορίας και της θερμοαλατικής συμπεριφοράς της λιμνοθάλασσας Κοτυχιού που βρίσκεται στη δυτική Πελοπόννησο. Το Κοτύχι είναι μια πολύ ρηχή λιμνοθάλασσα (μέσο βάθος 40 εκ) που χρησιμοποιείται σαν ένα φυσικό ιχθυοτροφείο εδώ και αιώνες. Είναι ένας βιότοπος πολύ μεγάλης οικολογικής και οικονομικής αξίας και προστατεύεται από τη διεθνή συνθήκη Ramsar. Δυστυχώς, η λ/θ Κοτυχιού έχει υποβαθμιστεί σημαντικά κατά την διάρκεια των 50 τελευταίων χρόνων εξαιτίας έντονων αγροτικών δραστηριοτήτων στις πλησίον περιοχές, ενώ η κατασκευή του φράγματος του Πηνιού στα τέλη του 60’ προκάλεσε σημαντικές προσχωματικές καταστροφές. Η μοντελοποίηση των φυσικών και οικολογικών διεργασιών στο Κοτύχι θα μπορούσε να αποτελέσει ένα πολή σημαντικό διαχειριστικό εργαλείο στα χέρια των ερευνητών που επιθυμούν να προτείνουν λύσεις σε οικολογικά προβλήματα παρόμοιας φύσης. Πρωταρχικός σκοπός της παρούσας διπλωματικής είναι να στήσει ένα αξιόπιστο υδροδυναμικό μοντέλο μέσα από την επιτυχημένη βαθμονόμηση και επιβεβαίωσή του. Αυτό το μοντέλο θα αποτελέσει τη βάση για προσομοιώσεις της διάχυσης της αλατότητας και της θερμοκρασίας, οι οποίες ομοίως θα λάβουν χώρα. Η βαθμονόμηση του μοντέλου πραγματοποιήθηκε με τη βοήθεια των μετρήσεων τριών μόνιμων σταθμών παρατήρησης (της ΤΕΔΚ Αχαΐας), οι οποίες φιλτραρίστηκαν κατάλληλα. Οι δύο σταθμοί βρίσκονταν στο εσωτερικό της λ/θ και ο τρίτος στο εσοδευτικό στόμιό της. Μετά από την επιτυχή βαθμονόμηση, τα αποτελέσματα του μοντέλου βρίσκονται σε πολύ καλή συμφωνία με τις χωρικές κατανομές που κατασκευάστηκαν με δειγματοληψία πεδίου προηγούμενων ημερών. Έπειτα, μετεωρολογικά και υδροδυναμικά δεδομένα ποικίλων θερινών και χειμερινών περιόδων χρησιμοποιήθηκαν για την πρόβλεψη των υδροδυναμικών και θερμοαλατικών συνθηκών στη λιμνοθάλασσα. Μια εκτεταμένη μελέτη του θερμικού ισοζυγίου μεταξύ νερού και ατμόσφαιρας επίσης έλαβε χώρα με αποτελέσματα που συμφωνούν με τις υπαίθριες μετρήσεις. / Two dimensional modeling methods are applied to simulate the water transport, temperature and salinity in Kotychi Lagoon, Western Peloponnese, Greece. Kotychi is a very shallow lagoon (average depth 40 cm) that is used as a natural fish farm since centuries. It is a biotope of great ecological and financial importance and is protected by the Ramsar International Convention. Unfortunately, it has been severely degraded and transformed during the past 50 years due to agricultural activities in the surrounding areas and watercourse alteration after the construction of Pinios river dam in the late 60’s. Modeling of physical and ecological processes in Kotychi lagoon could be a great managerial tool for researchers who wish to propose solutions at such ecological problems. The main purpose of the present study is to set up a reliable hydrodynamic model, by successfully calibrating and validating it. This model will be the base for simulations of the temperature and salinity dispersions which will also be carried out.The calibration of the model has been performed against the filtered measurements of three tide gauges, two of them located inside the Lagoon and one at its mouth. After calibration, model output shows a very good agreement with a set of data referring to a period of average winds and with spatial distributions created by field measurements at particular days. Meteorological and hydrological data are then used to set up a week long simulations that model the temperature and salinity of various winter and summer periods. An extended study of the water-air heat exchange of the lagoon has also been carried out with success.

A mathematical model of a steam-methane reformer (SMR) was developed for use in process performance simulations and on-line monitoring of tube-wall temperatures. The model calculates temperature profiles for the outer-tube wall, inner-tube wall, furnace gas and process gas. Reformer performance ratios and composition profiles are also computed. The model inputs are the reformer inlet-stream conditions, the geometry and material properties of the furnace and catalyst-bed. The model divides the furnace and process sides of the reformer into zones of uniform temperature and composition. Radiative-heat transfer on the furnace side is modeled using the Hottel Zone method. Energy and material balances are performed on the zones to produce non-linear algebraic equations, which are solved using the Newton-Raphson method with a numerical Jacobian. Model parameters were ranked from most-estimable to least estimable using a sensitivity-based estimability analysis tool, and model outputs were fitted to limited data from an industrial SMR. The process-gas outlet temperatures were matched within 4 ºC, the upper and lower peep-hole temperatures within 12 ºC and the furnace-gas outlet temperature within 4 ºC. The process-gas outlet pressure, composition and flow rate are also accurately matched by the model. The values of the parameter estimates are physically realistic. The model developed in this thesis has the capacity to be developed into more specialized versions. Some suggestions for more specialized models include modeling of separate classes of tubes that are in different radiative environments, and detailed modeling of burner configurations, furnace-gas flow patterns and combustion heat-release patterns. / Thesis (Master, Chemical Engineering) -- Queen's University, 2009-01-06 21:50:35.04

Replacing humans with underwater robots for accomplishing marine tasks such as oceanic supervision and undersea operations have been an endeavour from long time ago. Hence, a number of underwater robots have been developed. Among those underwater robots, developing biomimetic swimming robots has been appealing for many researchers and institutes since these robots have shown superior performance. Biomimetic swimming robots have higher swimming efficiency, manoeuvrability and noiseless performance. However, the existing biomimetic swimming robots are specialised for a single gait of locomotion like cruising, manoeuvrability and accelerating while for efficient accomplishment of marine tasks, an underwater robot needs to have multiple gaits of locomotion. In order to develop multiple-gaited swimming robots, the optimal characteristics of each gait of swimming must be combined together, whereas the combination is not usually possible. The problem needs to be addressed during the design process. Moreover, the optimality of the actuation mechanism of robots - that do not utilise any artificial muscle - could be assured using the mathematical model employed for simulation of their swimming behaviour. However, the existing models are incomplete and, accordingly, not reliable since their assumptions like the constant speed of flow around the fish robot could be used when the average speed of the flow is determined during experiment while before development of robots, the flow speed is not known. In addition to that, the simulation results must be optimised using the experimental observations in nature and analytical results while the optimisation algorithms are based on one fitness function. The aforementioned problems as well as the fabrication challenges of free-swimming biomimetic robots are addressed in a development process of multiple-gaited fish-mimetic robots introduced by the author in this thesis. This development method engages the improvement of all development steps of fish robots including design, mathematical modelling, optimisation and fabrication steps. In this thesis, the aforementioned steps are discussed and the contributions of the method for each step are introduced. As an outcome of the project, two prototypes of fish robots called UC-Ika 1 & 2 are built.

An increasing number of contemporary studies in aquatic ecology emphasize the im- portance of highly unsaturated fatty acids (HUFAs) at the plant-animal interface. Studies have demonstrated a wide range of fatty acid profiles in primary producers, forcing her- bivorous zooplankton to differentially retain fatty acids to meet somatic requirements. Herbivores also vary in their somatic fatty acid profiles; cladocerans collect Eicosapen- taenoic Acid (EPA), copepods prefer Docosahexaenoic Acid (DHA). Fatty acid internal reserves can be broken down to meet structural needs (i.e. phospholipid synthesis), fuel reproduction and may play a role in cold weather adaptation. Several authors have noted increases in HUFA concentration with lowering ambient temperatures. Cladoceran membranes form a gel at lower temperatures, while copepod membranes remain fluid and allow active overwintering. Both fish and crustaceans accumulate high concentrations of HUFAs during periods of rapid growth, but colimitation with elemental resources may exist. Recent modeling results suggest food webs with high quality (nutritional and biochemical) primary producers can attain inverted biomass distributions with efficient energy transfer between trophic levels. The adoption rate for this material into man- agement studies remains low, and while other sectors of the scientific community thrive on the potential of HUFAs, planktonic food-web studies are choosing traditional view points over forward thinking. Bearing in mind the emerging hypotheses on the critical factors that drive the energy flow in the plant-animal interface, my dissertation will at- tempt to address the following general questions: What are the distinct signatures of food quality and food quantity on planktonic food web dynamics? How do nutritional and biochemical factors affect the flow of energy at the plant-animal interface? What is our current understanding of the role of highly unsaturated fatty acids (HUFAs) in aquatic food webs? To what extent can the current generation of plankton models reproduce the lower food web patterns when explicitly accounting for HUFAs? Is the integration of the HUFA role into water quality management models feasible? Explicitly accounting for HUFAs requires integrating factors of animal physiology with macro-ecology: what are the ramifications? Finally, what are the evolutionary aspects of animals coping with food quality?

An increasing number of contemporary studies in aquatic ecology emphasize the im- portance of highly unsaturated fatty acids (HUFAs) at the plant-animal interface. Studies have demonstrated a wide range of fatty acid profiles in primary producers, forcing her- bivorous zooplankton to differentially retain fatty acids to meet somatic requirements. Herbivores also vary in their somatic fatty acid profiles; cladocerans collect Eicosapen- taenoic Acid (EPA), copepods prefer Docosahexaenoic Acid (DHA). Fatty acid internal reserves can be broken down to meet structural needs (i.e. phospholipid synthesis), fuel reproduction and may play a role in cold weather adaptation. Several authors have noted increases in HUFA concentration with lowering ambient temperatures. Cladoceran membranes form a gel at lower temperatures, while copepod membranes remain fluid and allow active overwintering. Both fish and crustaceans accumulate high concentrations of HUFAs during periods of rapid growth, but colimitation with elemental resources may exist. Recent modeling results suggest food webs with high quality (nutritional and biochemical) primary producers can attain inverted biomass distributions with efficient energy transfer between trophic levels. The adoption rate for this material into man- agement studies remains low, and while other sectors of the scientific community thrive on the potential of HUFAs, planktonic food-web studies are choosing traditional view points over forward thinking. Bearing in mind the emerging hypotheses on the critical factors that drive the energy flow in the plant-animal interface, my dissertation will at- tempt to address the following general questions: What are the distinct signatures of food quality and food quantity on planktonic food web dynamics? How do nutritional and biochemical factors affect the flow of energy at the plant-animal interface? What is our current understanding of the role of highly unsaturated fatty acids (HUFAs) in aquatic food webs? To what extent can the current generation of plankton models reproduce the lower food web patterns when explicitly accounting for HUFAs? Is the integration of the HUFA role into water quality management models feasible? Explicitly accounting for HUFAs requires integrating factors of animal physiology with macro-ecology: what are the ramifications? Finally, what are the evolutionary aspects of animals coping with food quality?