The research and development of zinc selenide (ZnSe) based wide-gap II¡VVI materials and related blue/green light-emitting devices have significantly progressed since 1990. ZnSe is also a promising material for use in windows, lenses, output couplers, beam expanders, optically controlled switching, visible transmission and giant photo-resistivity. Recently, ZnSe has become an important material used as the window layer of CuInSe2-based solar cells . To improve device performance, it is necessary to reduce interface defects and improve epitaxial layer quality. However, ZnSe thin films on glass substrates with high reliability and applicability have not yet been developed. In this study, a ZnSe buffer layer was firstly grown by SME during the initial stage of film growth; the film crystallinity could gradually be improved layer by layer with smaller thicknesses. While the lattice disorder of the buffer layer decreased rapidly after several cycles of SME growth, the interfacial lattice mismatch between the buffer layer and the ZnSe film deposited by MBD was reduced. Thus, ZnSe films with buffer layers demonstrated better crystallinity. Initial growth stage evaluation of high-quality ZnSe films deposited on glass substrate was investigated. The self-limiting monolayer epitaxial (SME) process was used to pre-growth the buffer layer for a zinc selenide (ZnSe) film deposited. After alternating depositions for several cycles, the growth mode was changed to the molecular beam deposition (MBD) mode under growth conditions. Films deposited at substrate temperatures of 250¡V350¢XC and Se/Zn beam equivalent pressure (BEP) ratios of 0.77¡V1.87 were investigated. The crystal structure and preferred orientation of as-grown ZnSe films were examined using X-ray diffraction (XRD) patterns. The optical properties of the ZnSe films were revealed by photoluminescence spectra. The structure properties of as-deposited ZnSe films have been measured by X-ray diffraction (XRD) technique. The optimum film growth condition has been determined rapidly by comparing and analyzing the relative full width at half-maximum (FWHM) and peak intensity of XRD spectra. The composition of ZnSe films is determined by energy dispersive spectroscopic (EDS) analysis. Optical properties of ZnSe films are characterized by photoluminescence spectra. In addition, the structural parameters, crystallinity, lattice constant, grain size, strain, dislocation density and orientation of ZnSe film calculated are correlated with their growth conditions. The characteristics of the ZnSe films with and without a buffer layer were compared and discussed in detail. Finally, our results demonstrate that how the quality of ZnSe film can be improved on glass substrates for application to various devices.

La méthylation de l’ADN est un phénomène épigénétique qui joue un rôle important dans le développement des mammifères et qui est associé à une répression transcriptionnelle. La méthylation de loci CpG de l’ADN est médiée par les méthyltransférases de l’ADN – les Dnmts. La méthylation joue également un rôle clef dès les stades précoces de la cancérogenèse dans une grande partie des tumeurs où on observe une méthylation, notamment la répression des gènes suppresseurs de tumeurs et une déméthylation, notamment l’expression de séquences d’ADN parasites. Dans une première partie de la thèse, nous nous sommes intéressés à la méthyltransférase de l’ADN Dnmt3L et plus particulièrement à identifier les mécanismes par quels cette méthyltransférase peut réprimer l’expression génique. Dnmt3L, identifiée et clonée en 2000, est caractérisée comme une méthyltransférase dépourvue de son domaine catalytique jouant probablement un rôle dans la régulation de la méthylation de l’ADN plutôt que dans l’ajout de groupes méthyls à l’ADN. Son rôle est fondamental dans l’établissement de l’empreinte génétique maternelle. Des travaux récents, réalisés, notamment par notre équipe, ont permis de montrer que plusieurs Dnmt répriment la transcription non seulement en méthylant l’ADN mais également en interagissant avec les déacétylases d’histones, HDAC. Au regard de ces résultats, nous nous sommes intéressés à évaluer si Dnmt3L est également capable de réprimer la transcription en recrutant une activité HDAC. Des recherches menées de concert avec Rachel Deplus, étudiante en thèse au laboratoire, ont permis de montrer que Dnmt3L interagit avec les déacétylases d’histones, ce qui conduit à la répression de la transcription. Dans l’ensemble, nos résultats ont permis de montrer que Dnmt3L, bien que dépourvue d’activité méthyltransférase, peut, tout comme les autres Dnmt, également réprimer la transcription par le biais de son interaction avec les enzymes HDAC. Dans une deuxième partie majeure de la thèse, nous nous sommes intéressés à l’étude des mécanismes par lesquels la méthylation de l’ADN peut être ciblée au sein du génome. Cette thématique bien que fondamentale, est encore peu connue à l’heure actuelle. Des études très récentes, réalisées dans notre laboratoire, suggèrent que les Dnmt peuvent être recrutées au sein de loci spécifiques suite à leur association avec des facteurs de transcription. Dans le cadre de ma thèse, nous avons pu montrer que la méthyltransférase de l’ADN Dnmt3a interagit in vitro et in vivo avec l’oncoprotéine Myc. Nous avons également pu mettre en évidence que la protéine Myc endogène recrute une activité méthyltransférase de l’ADN. Bien que l’oncoprotéine Myc soit surtout connue pour être un activateur de la transcription, Myc est également décrit pour réprimer la transcription de certains gènes spécifiques. Il était donc raisonnable de proposer que Dnmt3a pourrait être recruté pour réprimer les gènes régulés négativement par Myc. En effet en testant l’activité promotrice du gène p21, un gène connu pour être réprimé par Myc, nous avons démontré que Dnmt3a agit comme un co-répresseur transcriptionnel de Myc. Par des essais d’immunoprécipitation de la chromatine (ChIP), nous avons démontré que Myc et Dnmt3a forment un complexe stable sur le promoteur du gène p21. D’autre part nous avons montré que la 5 azacytidine, un agent déméthylant, lève la répression médiée par Myc au sein du promoteur du gène p21. En collaboration avec d’autres laboratoires, nous avons également effectué du séquençage au bisulfite du promoteur proximal de ce gène à partir d’ADN provenant de cellules sauvages pour Myc (myc+/+) ou invalidées pour Myc (myc-/-), montrant que la méthylation de ce promoteur est dépendante de la présence de Myc. Ainsi il semble que l’activité méthyltransférase de l’ADN de Dnmt3a soit requise pour sa fonction de co-répresseur des gènes régulés négativement par Myc. Cette étude confirme et valide le nouveau concept du recrutement des Dnmt par des interactions protéine-protéine. Notre travail a également des implications sur la compréhension du rôle de la méthylation de l’ADN dans la tumorigenèse. De plus, cette étude a permis de montrer pour la première fois que l’oncoprotéine Myc n’est pas seulement impliquée dans une répression génique passive (séquestration de co-activateurs) mais également une répression active (recrutement du co-represseur Dnmt3a).

The nucleosome remodeling and deacetylase (NuRD) complex is an abundant deacetylase complex, which couples histone deacetylation and chromatin remodeling ATPase activities, and has a broad cellular and tissue distribution. Although the working model of how this complex forms and functions is not well known, we have demonstrated that the coiled-coil interaction between two proteins (MBD2 and p66α) is critical for DNA methylation dependent gene silencing in vivo. Chapter one: ‘Unique features of the anti-parallel, heterodimeric coiled-coil interaction between methyl-cytosine binding domain 2 (MBD2) homologues and p66α dictate high affinity binding’ describes this unique coiled coil interaction. Coiled-coils were studied using a variety of biophysical techniques including analytical ultracentrifugation (AUC), isothermal titration calorimetry (ITC) and circular dichroism (CD). Results were compared across homologues and mutation studies were carried out to test our hypotheses. The studies reported in this chapter add to our understanding of coiled-coil interaction and thereby facilitate development of small peptide based drugs which target such interactions in nature.A number of proteins have been identified in humans that specifically bind to methylated CpG via a methyl binding domain (MBD). The human genome encodes at least five MBD proteins: MeCP2 and MBD1 through MBD4, which are homologous in their methyl binding domains but not many similarities are seen outside the MBD. Out of the five MBDs, MBD4 has a c-terminal glycosylase domain through which it recognizes mCpG.TpG mismatch and is important for base excision repair system. Chapter two: ‘Dynamic behavior of MBD4 in methylated DNA recognition’ focuses on MBD4 and its preference for DNA methylation mark. Techniques of surface plasmon resonance (SPR), nuclear magnetic resonance (NMR) spectroscopy are used to study binding affinity for variations of methylated DNA mark. Chemical exchange studies are used to demonstrate how MBD4 scans for methylation mark and these studies have added a new dimension to our understanding of how MBD proteins ‘read’ DNA methylation marks. Chapter three: ‘Solving the solution structure of MBD domain of MBD4 on methylated DNA by NMR’ describes a process of structure determination using NMR spectroscopy. The focus of this chapter is not on developing a new technique but rather on using current resources to solve a protein structure, which can be used to further understand our biological system. Here, I have discussed the workflow used to determine a final three-dimensional structure starting from sample preparation, data collection, data analysis to structure calculation.

<p>Aircraft developers like other development and manufacturing companies, are experiencing increasing complexity in their products and growing competition in the global market. One way to confront the challenges is to make the development process more efficient and to shorten time to market for new products/variants by using design and development methods based on models. Model Based Systems Engineering (MBSE) is introduced to, in a structured way, support engineers with aids and rules in order to engineer systems in a new way.</p><p>In this thesis, model based strategies for aircraft and avionics development are studied. A background to avionics architectures and in particular Integrated Modular Avionics is described. The integrating discipline Systems Engineering, MBSE and applicable standards are also described. A survey on available and emerging modeling techniques and tools, such as Hosted Simulation, is presented and Modeling Domains are defined in order to analyze the engineering environment with all its vital parts to support an MBSE approach.</p><p>Time and money may be saved by using modeling techniques that enable understanding of the engineering problem, state-of-the-art analysis and team communication, with preserved or increased quality and sense of control. Dynamic simulation is an activity increasingly used in aerospace, for several reasons; to prove the product concept, to validate stated requirements, and to verify the final implementation. Simulation is also used for end-user training, with specialized training simulators, but with the same underlying models. As models grow in complexity, and the set of simulation platforms is expanded, new needs for specification, model building and configuration support arise, which requires a modeling framework to be efficient.</p>

<p>This master thesis is Atlas Copco Craelius’ first step in incorporating numericalmethods in load case analysis during the development process. Atlas Copco needs toconstantly evolve and refine their methods in the design process to remain as thenumber one manufacturer of mining and construction equipment. Poor knowledge ofloads results either in structural failures or in oversized structures, both very costlyfor Atlas Copco.The main goal of this thesis is to examine the potential in MBS software by using it toidentify extreme load cases in one of Atlas Copco Craelius’ surface drill rigs, Mustang5. The MBS-software ADAMS View is used to build a model of the Mustang 5 drill rigand evaluate simulation results. The rig model is subject to motions which representreal case scenarios. The feed positioning possibilities are examined thoroughly since it was expected that the positioning has impact on the load levels. 25 different feedpositioning are simulated.The main conclusion is that the load levels are highly dependent on the feedpositioning. For example, the load levels in the boom raising cylinder can increaseseven times, if the rig is operated with the most unfavorable positioning compared tothe recommended. It could also be concluded that not only one positioning isextreme in terms of loads; it depends on which part of the boom system is beingobserved. Thus, several positioning cases have to be taken into consideration in orderto optimize the design of parts in the boom system.</p>

This master thesis is Atlas Copco Craelius’ first step in incorporating numericalmethods in load case analysis during the development process. Atlas Copco needs toconstantly evolve and refine their methods in the design process to remain as thenumber one manufacturer of mining and construction equipment. Poor knowledge ofloads results either in structural failures or in oversized structures, both very costlyfor Atlas Copco.The main goal of this thesis is to examine the potential in MBS software by using it toidentify extreme load cases in one of Atlas Copco Craelius’ surface drill rigs, Mustang5. The MBS-software ADAMS View is used to build a model of the Mustang 5 drill rigand evaluate simulation results. The rig model is subject to motions which representreal case scenarios. The feed positioning possibilities are examined thoroughly since it was expected that the positioning has impact on the load levels. 25 different feedpositioning are simulated.The main conclusion is that the load levels are highly dependent on the feedpositioning. For example, the load levels in the boom raising cylinder can increaseseven times, if the rig is operated with the most unfavorable positioning compared tothe recommended. It could also be concluded that not only one positioning isextreme in terms of loads; it depends on which part of the boom system is beingobserved. Thus, several positioning cases have to be taken into consideration in orderto optimize the design of parts in the boom system.

We use molecular beam deposition (MBD) system to grow CuAlSe2 thin film. The films have been characterized by electrical measurements but also by X-ray diffraction, electron probe microanalysis, optical measurements, scanning electron microscopy and photoluminescence. It is shown that CuAlSe2 thin film is chalcopyrite structure with a band gap of 2.65eV, p-type conductivity and the smallest resistivity is 1.26¡Ñ102(

This work highlights the design process to build a MBD (Multi-Body Dynamics) model with flexible parts for a RCV (Research Concept Vehicle). Full vehicle dynamic simulations of the RCV model with flexible parts were performed for different load cases and the results were compared with that of a MBD model with rigid body components. In addition, FE modelling of the RCV body parts, selection of attachment nodes, generation and verification of Modal Neutral Files (MNFs) are discussed. RCV is a concept vehicle developed at KTH Royal Institute of technology as a research platform to implement, validate and demonstrate results of various research projects. The vehicle consists of body, suspension and tire subsystems which were designed and developed as individual projects. The body subsystem comprises of rollcage, subframe and a composite baseplate. In this project, a MBD model of the RCV was developed in ADAMS/CAR to measure the forces acting at the interface of these body components and also to consider the suspension forces acting on the individual front and rear subframe parts. Finite element (FE) models were incorporated to consider the flexibility of the body components. The RCV is a vehicle constantly evolving with addition of new components to implement and test various research results. To study the application of this method, two Models of the RCV with design modifications were developed and studied. A model of the RCV without rollcage and a model with a rigid link connecting the body components were built and the results of dynamic simulations were compared with that of the existing RCV design. When flexibility of the baseplate was considered in the models, an overall change in dynamics of the body components was observed. Further, observing the results from models with design modifications, it was evident that this method can be used to study the effect of these modifications on the dynamic behaviour of the vehicle. / Det här arbetet belyser konstruktionsprocessen för att bygga en MBD-modell (Multi-Body Dynamics) med flexibla komponenter av konceptfordonet RCV (Research Concept Vehicle). Fullständiga fordonsdynamiska simuleringar med flexibla komponenter utfördes för olika lastfall och resultaten jämfördes med en MBD-modell med stela komponenter. Dessutom diskuteras FE modellering av RCVs olika delsystem, val av kopplingsnoder, generering och verifiering av ”Modal Neutral Files” (MNFs). RCV är ett konceptfordon som utvecklats vid Kungliga Tekniska Högskolan, KTH, som en forskningsplattform för att implementera, validera och demonstrera resultaten av olika forskningsprojekt. Fordonet består av delsystemen; chassi, hjulupphängning, och däck, vilka har utvecklats tidigare i separata projekt. Chassit består i sin tur av delsystemen; ”rollcage”, ”subframe” och ”baseplate”. I detta projekt har en MBD-modell av RCV utvecklats i ADAMS/CAR för att simulera olika körfall och beräkna de krafter som verkar mellan dessa delsystem och att också studera skillnaden i belastning av främre resp. bakre ”subframe”. FE modeller importeradesäven till modellen för att studera effekten av elasticiteten hos komponenterna på fordonets beteende.RVC är ett fordon som konstant utvecklas med tillägg av nya komponenter för att implementera och testa olika forskningsresultat. För att studera tillämpningen av denna metod skapades två modeller av RCV med olika konstruktiva förändringar vilkas inverkan på fordonet studerades. En modell av RCV utan ”rollcage” och en modell med styv länk som förbinder olika delar av chassit skapades och resultaten av dynamiska simuleringar jämfördes med simuleringsresultat för den befintliga RCV-designen. När flexibiliteten hos basplattan beaktades i modellerna observerades förändringar i dynamiken hos chassit vad gäller vertikala förskjutningar och vinkelförskjutningar. Utifrån dessa simuleringar kan vi dra slutsatsen att den utvecklade metoden är användbar för att studera effekter av konstruktionsförändringar på det dynamiska beteendet hos fordonet.

The main aim of this master thesis is to improve the performance of the Research Concept Vehicle (RCV). The RCV is an electric over-actuated vehicle developed at Integrated Transport Research Lab (ITRL) at KTH Royal Institute of Technology. The vehicle steer, camber, drive, and brake on each wheel of the vehicle. The RCV also has various operation modes such as 2WD, 4WD, 2WS and 4WS. The RCV is used as a research platform to implement, validate, and demonstrate results of various research projects.  The RCV was developed in the year 2012. There is now a requirement to improve the performance of the vehicle to create a more dynamically capable platform to do more dynamic tests with. The main aim of this thesis is to explore the possibility of upgrading the suspension system with integrated wheel hub motor, electric steering actuator and electric camber actuator. It also involves packaging of the new battery pack system and reinforcing the chassis to reduce the flex during operation.  Steps followed involves analysis of the current electric steering and electric camber actuator systems using MBD method to test the performance. With this as the base, requirements are decided as to what must be done to improve the performance by creating another MBD model to obtain the new performance figures. Also, the new battery pack is to be positioned on the base plate of the vehicle and this is achieved by placing the new battery pack onto the existing CAD model. The chassis is to be reinforced with the help of cross members, also designed on CAD software. The damper unit needs to be repositioned to accommodate the battery pack. Based on the changes in the vehicle, new hardpoints are decided for the new steering system, camber system and suspension system.  Based on the new performance figures obtained from MBD, the requirements of the new electric steering and camber actuator systems are presented. The strength in the new frame is tested using FEM method. The new position of the damper unit is tested for performance using an MBD software.  With the end of this thesis, the requirements to develop the new and improved RCV was obtained, thereby allowing for more dynamic testing to be done with the electric vehicle. / Huvudsyftet med detta examensarbete är att förbättra prestanda för Research Concept Vehicle (RCV). RCV är ett elektriskt överaktuerat fordon utvecklat vid Integrated Transport Research Lab (ITRL) vid KTH Royal Institute of Technology. Fordonet styr, reglerar cambervinkeln, kör och bromsar med varje hjul i fordonet. RCV har också olika driftlägen som 2WD, 4WD, 2WS och 4WS. RCV används som en forskningsplattform för att implementera, validera och demonstrera resultat från olika forskningsprojekt.  RCV utvecklades år 2012. Nu är det nu ett krav att förbättra fordonets prestanda för att skapa en mer dynamiskt kapabel plattform att göra mer dynamiska tester med. Huvudsyftet med denna avhandling är att undersöka möjligheten att uppgradera upphängningssystemet med integrerad hjulnavmotor, elektrisk styrmanöverdon och elektrisk cambermanöverdon. Det handlar också om förpackning av det nya batteripaketet och förstärkning av chassit för att minska flex under drift.  Stegen som följs innefattar analys av de nuvarande elektriska styrsystemen och de elektriska camber-ställdonssystemen med MBD-metoden för att testa prestandan. Med detta som bas bestäms krav på vad som måste göras för att förbättra prestandan genom att skapa en annan MBD-modell för att erhålla de nya prestandasiffrorna. Det nya batteripaketet ska också placeras på fordonets bottenplatta med hjälp av CAD-programvara. Chassit ska förstärkas med hjälp av tvärbalkar, även utformade på CAD-programvara. Spjällenheten måste placeras om för att rymma batteripaketet. Baserat på förändringarna i fordonet bestäms nya hårda punkter för det nya styrsystemet, camber-systemet och upphängningssystemet.  Baserat på de nya prestandasiffrorna som erhållits från MBD presenteras kraven för de nya elektriska styr- och camber-ställdonssystemen. Styrkan i den nya ramen testas med FEM-metoden. Spjällenhetens nya position testas för prestanda med hjälp av en MBD-programvara.  I slutet av denna avhandling erhölls kraven för att utveckla den nya och förbättrade RCV, vilket möjliggjorde en mer dynamisk testning med elfordonet.

This thesis started as an initiative from one of the co-authors that previously worked at SAAB AB during summer 2018. During the summer she worked with the design process of In-process part Definition (IPPD) and an interest emerged for making it more efficient. The design process of IPPD (DPOI) is where a design article, designed in CATIA, become manufacturable and adapted for assembly. The DPOI can be seen as the interface between the department of Airframe development and Production at SAAB AB. The first step was to investigate the current DPOI and conduct a pre-study to find time-consuming activities.  The pre-study consisted of five interviews, an observational study and a time study were the aims was to collect employees' own opinions, approve a pre-defined workflow divided into twelve elements and find problem areas. Element 1.0-11.0 is tasks within the DPOI and element 12.0 is the first step in the review process called Checker. Element 4.0 and 8.0 were divided further into parallel activities where the operators in the time study performs either, e.g., E4.0 (macro) or E4.1 (manually). To find time-consuming activities a time study was performed. The authors of this thesis acted observers and clocked each element while three operators denoted A-C designed 24 IPPDs. The results from the time study showed that elements 1.0, 3.0, 4.1 and 7.0 were time-consuming and E4.1 had potential to become automated. The selection of 2-3 problems was carried out through two Weighted Sum Models (WSM) where criteria was defined and solutions was listed. Each solution was weighted to each criterion and got a total grade. The selected problems, based on the total grade, were: Documents and Combined macro. Documents and manuals for scenario 5, 6 and the entire design process of IPPD was developed to make new employees learning process more efficient. A draft macro for scenario 5 and new complete macros for scenario 1 and 6 was developed and used in the comparative study. The comparative study was conducted like the previous time study but instead the new developed macros was used to make E4.0 more efficient and eliminate E4.1. In the comparative study only E4.0 was clocked for all 24 IPPDs in the time study. The result showed that E4.0 has become average 60% more efficient for all IPPDs and the total time with the new developed macros for E4.0 vs E4.1 has become 14,3% more efficient. Problems and time-consuming activities has been found and improved. The performed comparative study shows that the DPOI can be minimized further in terms of time; there are possibilities to make more elements from the DPOI automated.