Thesis (M.Ed.)--University of Hong Kong, 1982. / Includes bibliographical references (leaf 104-109) Also available in print.

The reliability of genome analysis and proficiency of genetic manipulation in vivo and in vitro are increased by assignment of linkage groups to specific chromosomes, placement of centromeres, orientation with respect to telomeres, and linear alignment with respect to chromosomal features and dimensions. I undertook five studies aimed at integrating sorghum genomics and cytogenetics at several levels. The results help establish an entirely new "cyto-genomics" resource, impacts of which are likely to be broad. In the first study, I developed a FISH-based karyotyping system for Sorghum bicolor Moench. I used integrated structural genomic resources, including linkage maps and large-insert clonal libraries of sorghum genomic DNA to develop a 17-locus probe cocktail for simultaneous fluorescent in situ hybridization (FISH). This probe enabled facile identification of all chromosome pairs in mitotic chromosome spreads. Perhaps just as important, I established time-efficient means to select sorghum BAC clones for multi-probe FISH. Thus, an integrated cyto-genomics system for sorghum can be constructed without need of chromosome flow sorting or microdissection, both of which are difficult and costly. In the second study, hybridization of DNA clones from 37 different genomic regions enabled the assignment of linkage groups and orientation of linkage maps to chromosomes. Comparisons between genetic and physical distances throughout the genome enabled a new nomenclature for linkage group designation in sorghum. The results provide an integrated nomenclature system of Sorghum bicolor chromosomes and linkage groups. In the third study, I created high-resolution maps by FISH to pachytene bivalents for two linkage groups (B and H), and defined relationships between pericentromeric heterochromatin, centromeres, mapped markers and recombination rates. These relationships will help guide the development and use of sorghum genomics. In the fifth study, I used FISH in two ongoing gene-targeted efforts. For the maturity gene ma5 and fertility restoration gene rfl, I estimated physical lengths between currently available flanking molecular markers. This enables estimation of recombination densities in these regions and assessment of the applicability of map-based and -assisted cloning.

The reliability of genome analysis and proficiency of genetic manipulation in vivo and in vitro are increased by assignment of linkage groups to specific chromosomes, placement of centromeres, orientation with respect to telomeres, and linear alignment with respect to chromosomal features and dimensions. I undertook five studies aimed at integrating sorghum genomics and cytogenetics at several levels. The results help establish an entirely new "cyto-genomics" resource, impacts of which are likely to be broad. In the first study, I developed a FISH-based karyotyping system for Sorghum bicolor Moench. I used integrated structural genomic resources, including linkage maps and large-insert clonal libraries of sorghum genomic DNA to develop a 17-locus probe cocktail for simultaneous fluorescent in situ hybridization (FISH). This probe enabled facile identification of all chromosome pairs in mitotic chromosome spreads. Perhaps just as important, I established time-efficient means to select sorghum BAC clones for multi-probe FISH. Thus, an integrated cyto-genomics system for sorghum can be constructed without need of chromosome flow sorting or microdissection, both of which are difficult and costly. In the second study, hybridization of DNA clones from 37 different genomic regions enabled the assignment of linkage groups and orientation of linkage maps to chromosomes. Comparisons between genetic and physical distances throughout the genome enabled a new nomenclature for linkage group designation in sorghum. The results provide an integrated nomenclature system of Sorghum bicolor chromosomes and linkage groups. In the third study, I created high-resolution maps by FISH to pachytene bivalents for two linkage groups (B and H), and defined relationships between pericentromeric heterochromatin, centromeres, mapped markers and recombination rates. These relationships will help guide the development and use of sorghum genomics. In the fifth study, I used FISH in two ongoing gene-targeted efforts. For the maturity gene ma5 and fertility restoration gene rfl, I estimated physical lengths between currently available flanking molecular markers. This enables estimation of recombination densities in these regions and assessment of the applicability of map-based and -assisted cloning.

abstract: A volunteered geographic information system, e.g., OpenStreetMap (OSM), collects data from volunteers to generate geospatial maps. To keep the map consistent, volunteers are expected to perform the tedious task of updating the underlying geospatial data at regular intervals. Such a map curation step takes time and considerable human effort. In this thesis, we propose a framework that improves the process of updating geospatial maps by automatically identifying road changes from user-generated GPS traces. Since GPS traces can be sparse and noisy, the proposed framework validates the map changes with the users before propagating them to a publishable version of the map. The proposed framework achieves up to four times faster map matching performance than the state-of-the-art algorithms with only 0.1-0.3% accuracy loss. / Dissertation/Thesis / Masters Thesis Computer Science 2017

Made available in DSpace on 2018-08-02T00:03:48Z (GMT). No. of bitstreams: 1 tese_11160_CamilaZacche_dissertacao_final.pdf: 5437260 bytes, checksum: 0c96c6b2cce9c15ea234627fad78ac9a (MD5) Previous issue date: 2017-03-31 / 8 Resumo Os mapas conceituais são ferramentas gráficas para a representação e construção do conhecimento. Conceitos e relações formam a base para o aprendizado e, portanto, os mapas conceituais têm sido amplamente utilizados em diferentes situações e para diferentes propósitos na educação, sendo uma delas a represent ação do texto escrito. Mes mo um gramá tico e complexo texto pode ser representado por um mapa conceitual contendo apenas conceitos e relações que represente m o que foi expresso de uma forma mais complicada. No entanto, a construção manual de um mapa conceit ual exige bastante tempo e esforço na identificação e estruturação do conhecimento, especialmente quando o mapa não deve representar os conceitos da estrutura cognitiva do autor. Em vez disso, o mapa deve representar os conceitos expressos em um texto. Ass im, várias abordagens tecnológicas foram propostas para facilitar o processo de construção de mapas conceituais a partir de textos. Portanto, esta dissertação propõe uma nova abordagem para a construção automática de mapas conceituais como sumarização de t extos científicos. A sumarização pretende produzir um mapa conceitual como uma representação resumida do texto, mantendo suas diversas e mais importantes características. A sumarização pode facilitar a compreensão dos textos, uma vez que os alunos estão te ntando lidar com a sobrecarga cognitiva causada pela crescente quantidade de informação textual disponível atualmente. Este crescimento também pode ser prejudicial à construção do conhecimento. Assim, consideramos a hipótese de que a sumarização de um text o representado por um mapa conceitual pode atribuir características importantes para assimilar o conhecimento do texto, bem como diminuir a sua complexidade e o tempo necessário para processá - lo. Neste contexto, realizamos uma revisão da literatura entre o s anos de 1994 e 2016 sobre as abordagens que visam a construção automática de mapas conceituais a partir de textos. A partir disso, construímos uma categorização para melhor identificar e analisar os recursos e as características dessas abordagens tecnoló gicas. Além disso, buscamos identificar as limitações e reunir as melhores características dos trabalhos relacionados para propor nossa abordagem. 9 Ademais, apresentamos um processo Concept Map Mining elaborado seguindo quatro dimensões : Descrição da Fonte de Dados, Definição do Domínio, Identificação de Elementos e Visualização do Mapa. Com o intuito de desenvolver uma arquitetura computacional para construir automaticamente mapas conceituais como sumarização de textos acadêmicos, esta pesquisa resultou na ferramenta pública CMBuilder , uma ferramenta online para a construção automática de mapas conceituais a partir de textos, bem como uma api java chamada ExtroutNLP , que contém bibliotecas para extração de informações e serviços públicos. Para alcançar o objetivo proposto, direcionados esforços para áreas de processamento de linguagem natural e recuperação de informação. Ressaltamos que a principal tarefa para alcançar nosso objetivo é extrair do texto as proposições do tipo ( conceito, rela ção, conceito ). Sob essa premissa, a pesquisa introduz um pipeline que compreende: regras gramaticais e busca em profundidade para a extração de conceitos e relações a partir do texto; mapeamento de preposição, resolução de anáforas e exploração de entidad es nomeadas para a rotulação de conceitos; ranking de conceitos baseado na análise de frequência de elementos e na topologia do mapa; e sumarização de proposição baseada na topologia do grafo. Além disso, a abordagem também propõe o uso de técnicas de apre ndizagem supervisionada de clusterização e classificação associadas ao uso de um tesauro para a definição do domínio do texto e construção de um vocabulário conceitual de domínios. Finalmente, uma análise objetiva para validar a exatidão da biblioteca Extr outNLP é executada e apresenta 0.65 precision sobre o corpus . Além disso, uma análise subjetiva para validar a qualidade do mapa conceitual construído pela ferramenta CMBuilder é realizada , apresentando 0.75/0.45 para precision / recall de conceitos e 0.57/ 0.23 para precision/ recall de relações em idioma inglês e apresenta ndo 0.68/ 0.38 para precision/ recall de conceitos e 0.41/ 0.19 para precision/ recall de relações em idioma português. Ademais , um experimento para verificar se o mapa conceitual sumarizado pe lo CMBuilder tem influência para a compreensão do assunto abordado em um texto é realizado , atingindo 60% de acertos para mapas extraídos de pequenos textos com questões de múltipla escolha e 77% de acertos para m apas extraídos de textos extensos com quest ões discursivas

京都大学 / 新制・課程博士 / 博士(工学) / 甲第23151号 / 工博第4795号 / 新制||工||1750(附属図書館) / 京都大学大学院工学研究科都市社会工学専攻 / (主査)教授 澤田 純男, 教授 清野 純史, 准教授 後藤 浩之 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DFAM

Includes inset of Covington, Newport and vicinity. Includes hand-colored counties and handwritten note of C-1890 along with indications of railroad lines. Scale 1 in = 25 miles. / https://dc.etsu.edu/rare-maps/1155/thumbnail.jpg

Computers have vastly expanded capabilities for storing, creating, and manipulating spatial data, yet viewing area is still generally constrained to a single monitor. With this viewing window limitation, panning and zooming are required to view the full details of a map or image and, because of the large sizes of typical database, usually only in small portions. Multiple monitor configurations provide an attainable, low cost way for individuals to create large, high-resolution desktop displays. This increased screen real estate is particularly useful for viewing and interpreting rich and complex geospatial datasets because both context and amount of detail can be simultaneously increased, reducing reliance on virtual navigation to obtain the desired balance between context and scale. To evaluate the utility of multiple monitor displays for geospatial data, this experiment involved a variety of map and image reading tasks using both raster and vector data under three different monitor conditions: one monitor (1280 x 1024 pixels), four monitors (2560 x 2048 pixels), and nine monitors (3840 x 3072 pixels). Fifty-seven subjects took the test on one of the three display configurations. A computer program captured each subject's performance by recording answers, mouse click locations, viewing areas, tool usage, and elapsed time. A post-experiment questionnaire obtained additional qualitative feedback about subjects' experience with the tasks and display configuration. Overall, subjects did perform more efficiently on the larger display configurations as evidenced by a reduction in test completion time and in the amount of virtual navigation (mouse clicks) used to finish the test. Tool usage also differed among monitor conditions with navigation tools (zooming and panning) dominating on the single monitor while selecting tools (tools used to provide answers) predominated on the nine monitor display. While overall test results indicated the effectiveness of the larger displays, task-level analyses showed that specific performance varied considerably from task to task. The larger displays were the most efficient on some tasks, while other tasks showed similar results among all displays or even the single monitor as the most efficient. The best performance improvements occurred between the one and four monitor conditions, with the nine monitor condition mostly providing only modest additional improvement. Subjects rated the four monitor display size as the most ideal. / Master of Science

La voie ERK/MAPK est impliquée dans plusieurs processus biologiques dont la prolifération cellulaire, la différenciation, l'apoptose et la migration. Les protéines kinases MEK1 et MEK2 sont les seuls activateurs connus d'ERKl et ERK2. Les mutations inactivant les gènes Mekl et Mek2 ont été développées chez la souris pour étudier les fonctions des ces deux activateurs lors du développement embryonnaire. L'analyse phénotypique des mutants Mekl" montre une diminution de la prolifération et une augmentation de l'apoptose des populations trophoblastiques. Les embryons Mekïf~ meurent à mi-gestation d'une sous-vascularisation du labyrinthe placentaire qui normalement permet les échanges gazeux et nutritiormels foeto-maternels. Des analyses histologiques suggèrent également un problème de migration lors des processus d'invagination du réseau vasculaire. Ces résultats montrent que MEK1 est impliqué spécifiquement dans certaines fonctions des trophoblastes et que son absence induit une dysmorphogénèse létale du placenta murin. Pour étudier l'implication spécifique de MEK1 dans ces populations trophoblastiques, des modèles cellulaires ont été établis.

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