A major physiological risk factor of temporomandibular disorders (TMD) is sensitization of peripheral and central nervous system pain processing pathways. Calcium channel, voltage-dependent, alpha-2/delta subunit-1 (CACNA2D1) has a crucial role in relaying nociceptive information in the spinal dorsal horn. Up-regulation of CACNA2D1 results in abnormal excitatory synapse formation and enhanced presynaptic excitatory neurotransmitter release. Blocking CACNA2D1 with gabapentinoid-class drugs relieves orofacial hypersensitivity. Drs. Foley, Horton, and Sciote previously reported that in a small sample group (n=12), CACNA2D1 expression was greater in males than females, but increased in women with TMD. The objectives of this study are to corroborate these data and investigate expression patterns of other ion channel and conducting system genes. Additionally, since the null polymorphism ACTN3-577XX associates with muscle fiber microdamage during eccentric contraction, we tested for possible gene associations with ACTN3-R577XX genotypes. Masseter muscle samples came from human subjects (n=23 male; 48 female) with malocclusions undergoing orthognathic surgery. This population had skeletal disharmony of the jaws and thus was prone to eccentric contraction. Three males and eighteen females were diagnosed with localized masticatory myalgia. Muscle total RNA was isolated and CACNA2D1, CACNA1S, GABARAP, and TRPM7 expression was quantified using RT-PCR. Expression of these genes were compared based on TMD status and various characteristics that may influence TMD including: sex, age, facial symmetry, sagittal dimension, vertical dimension, ACTN3-577 genotype and fiber type. CACNA2D1 expression differed significantly between sexes, overall (p<0.02), and without TMD (p=0.001). Women with (n=13) and without (n=23) TMD differed significantly (p<0.03). CACNA2D1 expression was also significantly higher (p=0.031) in subjects below age 25. Similarly, GABARAP expression was significantly higher (p=0.001) for patients younger than 25 and for patients less than or equal to age 18 (p=0.013). Otherwise, CACNA1S, TRPM7 and GABARAP differences were not significant. GABARAP expression differed, but not significantly by sex and for the ACTN3-577XX-null genotype. In a population of malocclusion patients, masseter muscle CACNA2D1 expression is significantly higher than CACNA1S, TRPM7, and GABARAP. CACNA2D1 expression is greater in males than females without TMD. However, CACNA2D1 expression increases significantly in females with TMD-associated myalgia. This may support evidence for calcium channel regulation of nociception differences seen between sexes in TMD. It was also found that expression of CACNA2D1 and GABARAP is significantly higher in younger subjects. Additionally, observations presented here suggest potential influence of ACTN3-null condition on function of GABARAP. / Oral Biology

Objective: Obstructive sleep apnea (OSA) is defined by recurrent breathing cessations accompanied by a collapse of the pharyngeal airway. Co-morbid conditions include obesity, cardiovascular disease, diabetes, and in some cases, retrognathia and muscle dysfunction. The latter two conditions may prompt orthognathic correction. Past investigations have shown a genetic association with OSA. Given that masseter muscle influences skeletal malocclusion and is active during OSA, we investigated whether the expression of OSA-associated genes is altered in a Class II open bite OSA subject. Methods: Eleven mandibular advancement surgery patients were classified as skeletal Class II or III and open or deep bite malocclusion, including a Class II open bite patient with OSA. Masseter muscle samples were collected at surgery and frozen. Tissue was used for gene expression analysis on Affymetrix HT2.0 microarray chips and quantitative RT-PCR. Data for ten genes associated with OSA were individually evaluated in the microarray and compared between the OSA patient and eight symmetrical malocclusion subjects. In order to corroborate these expression data, one gene of interest, tumor necrosis factor (TNF), was quantified in the ten malocclusion subjects from the microarray, an OSA subject from the microarray and one additional OSA subject by RT-PCR. Results: Among OSA-associated genes on the microarray, interleukin genes IL1B, IL1R2, IL6 and IL8 were +2.5 to +9.2 fold greater (p < 0.02) and chemokine genes CCL2, CCL3, CCL3L3, CCL4 and CXCR1 were +2.0 to +12.1 fold greater (p < 0.05). Likewise, TNF expression differed significantly in the muscle of the OSA subject (+2.2 fold greater; p < 0.001). By quantitative RT-PCR, TNF expression was significantly greater in malocclusion subjects with OSA compared to those without OSA (p = 0.0004). Conclusions: Our findings support evidence that OSA is an inflammatory disorder, which may elicit hypoxia-induced inflammatory responses believed to promote skeletal muscle dysfunction. Specifically, we report that inflammatory gene expression is significantly increased in masseter muscle in Class II open bite subjects with OSA. In turn, malocclusion may contribute to OSA, which negatively affects masseter function, resulting in exacerbation of both disorders. Because OSA is reported to associate with a polymorphism in the TNF-alpha; gene in children, future studies are needed to test for similar genetic associations in malocclusion subjects with OSA. / Oral Biology

Para melhor compreender a fisiopatologia que afeta a musculatura do sistema estomatognático, a análise eletromiográfica dos músculos da mastigação tem sido incluída em muitos estudos. A eletromiografia (EMG) permite verificar e avaliar a eficácia muscular, entre os músculos de ambos os lados do corpo (simetria) e entre pares de músculos, com um possível efeito de desvio lateral da mandíbula (torque) (Ferrario et al., 1999, 2002). O objetivo desse trabalho foi estudar por meio da EMG de superfície os músculos masseter (porção superficial) e temporal (porção anterior) bilateralmente, e verificar o efeito do uso da placa oclusal resiliente, como um dos recursos para o tratamento das desordens temporomandibulares (DTMs), e comparar com um grupo controle. Foram envolvidos 23 indivíduos com sinais e sintomas de DTM, avaliados clinicamente, que receberam depois tratamento com placas oclusais resilientes. Para estabelecer a presença ou ausência de DTM, foi utilizado o Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD); além de registrar a atividade EMG dos músculos masseter e temporal, bilateralmente, antes (inicial Etapa 1), 30 (Etapa 2) e 60 dias após o uso da placa oclusal resiliente (Etapa 3) (Pettengill et al., 1998); relacionar os achados eletromiográficos com a avaliação clínica da oclusão e das funções estomatognáticas; comparar os resultados de uma população com DTM e o grupo controle de 23 indivíduos. As avaliações EMG foram registradas por meio de movimentos de mastigação e das condições clínicas de repouso, lateralidade com contato bilateral, protrusão e apertamento dental. A análise estatística foi realizada com o emprego do software SPSS versão 15.0 (Chicago, IL, USA). Devido ao fato de que foi rejeitada a hipótese de normalidade da grande maioria das variáveis (Teste de Shapiro-Wilks) foram utilizados métodos não-paramétricos na análise. O nível de significância adotado foi p ≤0,05. Os dados dos grupos controle e DTM foram comparados por meio do teste não-paramétrico de Mann-Whitney, e as comparações intragrupo DTM (inicial, 30 e 60 dias de uso de placa) foram realizadas por meio do teste não-paramétrico de Wilcoxon. De acordo com os resultados, na condição clínica de repouso, não houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM; e foi observado diferença estatisticamente significativa intra-grupo de DTM. Na condição clínica de lateralidade direita, houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM, e na comparação intra-grupo de DTM, não houve diferença estatisticamente significativa. Entretanto, na condição clínica de lateralidade esquerda, não houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM, e na comparação intra-grupo DTM. Na condição clínica de protrusão, na comparação entre os grupos controle e DTM, e na comparação intra-grupo de DTM, houve diferença estatisticamente significativa. Na condição clínica de apertamento de parafilme, houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM, e não houve diferença significativa na comparação intra-grupo de DTM. Nas provas de mastigação de Bis e amendoim, houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM, e não houve diferença estatisticamente significativa na comparação intra-grupo DTM. Para as provas de mastigação de chiclete e de parafilme, não houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM, e na comparação intra-grupo DTM. Pode-se concluir que a atividade eletromiográfica dos músculos masseter e temporal do grupo DTM diminuiu ao final do tratamento com uso de placa oclusal por 60 dias, ao se avaliar as condições clínicas de repouso (exceto para o músculo masseter direito), lateralidade direita e esquerda (para todos músculos); e protrusão (exceto para o músculo masseter esquerdo). Verificouse esta mesma diminuição para o apertamento dental com parafilme. / In order to understand better the physiopathology that affects the muscles of the stomatognathic system, an electromyography analyses has been included in the studies. The electromyography (EMG) allows us to check and evaluate the muscle efficiency, in both sides of the body (symmetry) and between the muscle pairs as a possible effect of the lateral jaw deviation (torque) (Ferrario et al., 1999, 2002). The aim of this work was to study, by surface EMG, the masseter muscles (superficial part) and temporal (back part) in both sides, as well as verifying the effect of the resilient occlusal splint use, as a devise for the temporomandibular disorder treatment (TMDs), and also compare it with the control group. We studied 23 individuals with TMD signs and symptoms, clinically evaluated, who received the resilient occlusal splint use treatment. We used the Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) in order to establish the presence or the absence of TMD; also it was registered an EMG activity of the masseter and temporal muscles, in both sides, before, (initial Step 1), 30 days (Step 2) and after 60 days of the resilient occlusal splint use (Step 3) (Pettengill et al., 1998); and relate the electromyography findings with the occlusion and stomatognathic functions clinic evaluation; compare the results of a population with TMD and the control group of 23 individuals. The EMG evaluation was registered through chewing movements and the rest clinic conditions, as well as bilateral laterality with contact, protrusion and tooth clench. The statistic analysis was done with the SPSS software version 15.0 (Chicago, IL, USA). Due to the fact that the hypothesis of the majority of the variable normality was rejected (Shapiro-Wilks Test) non-parametric methods were used in the analysis. The level of significance adopted p ≤0,05. The control group data and the TMD were compared by the non-parametric of Mann-Whitney test, and the intra-group comparisons TMD (initial, 30 and 60 days of splint use) were done with the non-parametric Wilcoxon test. According to the results in the rest clinic condition, there was no significant statistic difference between the control groups and TMD. It was seen a significant difference intra-group of TMD. There was a significant difference on the right laterality comparing both control groups and TMD, and there was no significant statistic difference in the intra-group comparison. Therefore, on the left laterality, we didnt find any significant difference between the control groups and the TMD either on the intra-group TMD comparison. We could find a quite significant difference between the protrusion clinic condition, in the comparison between the control groups and TMD, in the TMD intra-group comparison. Regarding the teeth clenching with parafilm clinic condition, there was a difference between the control groups and the TMD, but there was no difference between the TMD intra-group. The chewing test with Bis and peanuts, we could see a TMD group. We could see on the Bis test that there was a difference between the control group and the TMD, and there was no difference between the TMD intra-group. For the chewing gum test and of the parafilm, we didnt noticed difference between the control group, in the TMD and in the TMD intra-group. This way, we can conclude that the electromyography activity of the masseter and temporal muscles of the TMD group decreased at the end of the treatment with the use of the occlusal splint in 60 days, considering the rest clinic conditions (except for the right masseter muscle), right and left laterality (for all muscles), and protrusion (except for the left masseter muscle). It was verified the same decrease for the teeth clenching with parafilm.

Os objetivos do presente estudo foram analisar os efeitos do tratamento de pacientes com Desordem temporomandibular (DTM) articular crônica com a placa interoclusal estabilizadora modelo Michigan por meio dos índices eletromiográficos POC dos temporais, POC dos masseteres, TORS, ASSIM, Ativação, Ativação absoluta, TORQUE, Impacto, em máximo apertamento dental voluntário (MCV) em máxima intercuspidação habitual (MIH) e máximo apertamento voluntário com algodão entre os dentes (MAA). Foram também analisados os sinais e sintomas de DTM. Participaram do estudo 20 sujeitos, jovens e adultos, sendo 10 com DTM articular crônica, que receberão tratamento com placa oclusal modelo Michigan (Grupo DTM) e 10 sujeitos sem sinais e sintomas de DTM (Grupo Controle). Os sujeitos passarão por exame clínico e responderão ao Protocolo para Determinação dos Sinais e Sintomas de DTM para Centros Multiprofissionais (Felício et al., 2006). Os registros e cálculos dos índices eletromiográficos serão realizados com o Eletromiógrafo Freely de oito canais (De Götzen srl; Legano, Milano, Italy). Foram comparados os dados do grupo DTM na fase de diagnóstico (FD) e na fase final (FF) de tratamento, bem como os dados deste grupo com os do grupo controle. Para os dados expressos em nível intervalar de mensuração, como os dos exames clínicos, foi empregada estatística não-paramétrica. Os dados em nível de razão, isto é os dados eletromiográficos, foram analisados por meio de estatística paramétrica. O nível de significância estabelecido foi de 5%. / The objectives of this study were to analyze the effects of treatment of patients with temporomandibular disorder (TMD) joint with chronic plaque-stabilizing model interocclusal Michigan through electromyographic indices of temporal POC, the POC masseter, TORS, SO, Activation, Activation absolute TORQUE, Impact, for maximum voluntary tooth clenching (MCV) in maximum intercuspal usual (MHI) and maximum voluntary clenching with cotton between teeth (MAA). We also analyzed the signs and symptoms of TMD. The study included 20 subjects, young people and adults, and 10 with chronic articular TMD, which would be treated with occlusal splints Michigan model (DTM Group) and 10 subjects without signs and symptoms of TMD (control group). The subjects will undergo clinical examination and respond to the Protocol for the Determination of the signs and symptoms of TMD for multi Centers (Felicio et al., 2006). Records and calculations of indices electromyographic out with the electromyograph Freely eight channels (De Götzen srl; Legano, Milano, Italy). We compared the data from the DTM group stage of diagnosis (FD) and the final stage (FF) treatment, as well as data from this group with the control group. For data expressed as interval level of measurement, such as clinical examination, was used non-parametric statistics. Data on level of reason, ie electromyographic data were analyzed using parametric statistics. The significance level was set at 5%.

Para melhor compreender a fisiopatologia que afeta a musculatura do sistema estomatognático, a análise eletromiográfica dos músculos da mastigação tem sido incluída em muitos estudos. A eletromiografia (EMG) permite verificar e avaliar a eficácia muscular, entre os músculos de ambos os lados do corpo (simetria) e entre pares de músculos, com um possível efeito de desvio lateral da mandíbula (torque) (Ferrario et al., 1999, 2002). O objetivo desse trabalho foi estudar por meio da EMG de superfície os músculos masseter (porção superficial) e temporal (porção anterior) bilateralmente, e verificar o efeito do uso da placa oclusal resiliente, como um dos recursos para o tratamento das desordens temporomandibulares (DTMs), e comparar com um grupo controle. Foram envolvidos 23 indivíduos com sinais e sintomas de DTM, avaliados clinicamente, que receberam depois tratamento com placas oclusais resilientes. Para estabelecer a presença ou ausência de DTM, foi utilizado o Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD); além de registrar a atividade EMG dos músculos masseter e temporal, bilateralmente, antes (inicial Etapa 1), 30 (Etapa 2) e 60 dias após o uso da placa oclusal resiliente (Etapa 3) (Pettengill et al., 1998); relacionar os achados eletromiográficos com a avaliação clínica da oclusão e das funções estomatognáticas; comparar os resultados de uma população com DTM e o grupo controle de 23 indivíduos. As avaliações EMG foram registradas por meio de movimentos de mastigação e das condições clínicas de repouso, lateralidade com contato bilateral, protrusão e apertamento dental. A análise estatística foi realizada com o emprego do software SPSS versão 15.0 (Chicago, IL, USA). Devido ao fato de que foi rejeitada a hipótese de normalidade da grande maioria das variáveis (Teste de Shapiro-Wilks) foram utilizados métodos não-paramétricos na análise. O nível de significância adotado foi p ≤0,05. Os dados dos grupos controle e DTM foram comparados por meio do teste não-paramétrico de Mann-Whitney, e as comparações intragrupo DTM (inicial, 30 e 60 dias de uso de placa) foram realizadas por meio do teste não-paramétrico de Wilcoxon. De acordo com os resultados, na condição clínica de repouso, não houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM; e foi observado diferença estatisticamente significativa intra-grupo de DTM. Na condição clínica de lateralidade direita, houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM, e na comparação intra-grupo de DTM, não houve diferença estatisticamente significativa. Entretanto, na condição clínica de lateralidade esquerda, não houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM, e na comparação intra-grupo DTM. Na condição clínica de protrusão, na comparação entre os grupos controle e DTM, e na comparação intra-grupo de DTM, houve diferença estatisticamente significativa. Na condição clínica de apertamento de parafilme, houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM, e não houve diferença significativa na comparação intra-grupo de DTM. Nas provas de mastigação de Bis e amendoim, houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM, e não houve diferença estatisticamente significativa na comparação intra-grupo DTM. Para as provas de mastigação de chiclete e de parafilme, não houve diferença estatisticamente significativa na comparação entre os grupos controle e DTM, e na comparação intra-grupo DTM. Pode-se concluir que a atividade eletromiográfica dos músculos masseter e temporal do grupo DTM diminuiu ao final do tratamento com uso de placa oclusal por 60 dias, ao se avaliar as condições clínicas de repouso (exceto para o músculo masseter direito), lateralidade direita e esquerda (para todos músculos); e protrusão (exceto para o músculo masseter esquerdo). Verificouse esta mesma diminuição para o apertamento dental com parafilme. / In order to understand better the physiopathology that affects the muscles of the stomatognathic system, an electromyography analyses has been included in the studies. The electromyography (EMG) allows us to check and evaluate the muscle efficiency, in both sides of the body (symmetry) and between the muscle pairs as a possible effect of the lateral jaw deviation (torque) (Ferrario et al., 1999, 2002). The aim of this work was to study, by surface EMG, the masseter muscles (superficial part) and temporal (back part) in both sides, as well as verifying the effect of the resilient occlusal splint use, as a devise for the temporomandibular disorder treatment (TMDs), and also compare it with the control group. We studied 23 individuals with TMD signs and symptoms, clinically evaluated, who received the resilient occlusal splint use treatment. We used the Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) in order to establish the presence or the absence of TMD; also it was registered an EMG activity of the masseter and temporal muscles, in both sides, before, (initial Step 1), 30 days (Step 2) and after 60 days of the resilient occlusal splint use (Step 3) (Pettengill et al., 1998); and relate the electromyography findings with the occlusion and stomatognathic functions clinic evaluation; compare the results of a population with TMD and the control group of 23 individuals. The EMG evaluation was registered through chewing movements and the rest clinic conditions, as well as bilateral laterality with contact, protrusion and tooth clench. The statistic analysis was done with the SPSS software version 15.0 (Chicago, IL, USA). Due to the fact that the hypothesis of the majority of the variable normality was rejected (Shapiro-Wilks Test) non-parametric methods were used in the analysis. The level of significance adopted p ≤0,05. The control group data and the TMD were compared by the non-parametric of Mann-Whitney test, and the intra-group comparisons TMD (initial, 30 and 60 days of splint use) were done with the non-parametric Wilcoxon test. According to the results in the rest clinic condition, there was no significant statistic difference between the control groups and TMD. It was seen a significant difference intra-group of TMD. There was a significant difference on the right laterality comparing both control groups and TMD, and there was no significant statistic difference in the intra-group comparison. Therefore, on the left laterality, we didnt find any significant difference between the control groups and the TMD either on the intra-group TMD comparison. We could find a quite significant difference between the protrusion clinic condition, in the comparison between the control groups and TMD, in the TMD intra-group comparison. Regarding the teeth clenching with parafilm clinic condition, there was a difference between the control groups and the TMD, but there was no difference between the TMD intra-group. The chewing test with Bis and peanuts, we could see a TMD group. We could see on the Bis test that there was a difference between the control group and the TMD, and there was no difference between the TMD intra-group. For the chewing gum test and of the parafilm, we didnt noticed difference between the control group, in the TMD and in the TMD intra-group. This way, we can conclude that the electromyography activity of the masseter and temporal muscles of the TMD group decreased at the end of the treatment with the use of the occlusal splint in 60 days, considering the rest clinic conditions (except for the right masseter muscle), right and left laterality (for all muscles), and protrusion (except for the left masseter muscle). It was verified the same decrease for the teeth clenching with parafilm.

Nos dias atuais, uma significante parte da população nacional pode apresentar alterações da relação maxilo-mandibular, chamada de má oclusão. A principal alteração do crescimento facial encontrado nos adultos é a deficiência transversal da maxila. A correção desta deficiência é realizada por um procedimento cirúrgico denominado de expansão rápida da maxila cirurgicamente assistida. Estas cirurgias causam uma mudança na atividade muscular, principalmente naqueles músculos responsáveis pelo fechamento mandibular, no qual se destaca o músculo masseter. Estas alterações musculares duram em média 30 dias, quando clinicamente note-se a diminuição da força ou potencial muscular de fechamento mandibular. Verificam-se os efeitos da expansão rápida de maxila sobre os músculos masséteres dos pacientes, avaliando resultados obtidos por eletromiografia de superfície. Realizaram quatro avaliações, no 7° dia pré-operatório e no 7°, 30° e 60° dia pós-operatório, em 20 pacientes, durante a contração máxima voluntária isométrica sobre um rolete de algodão. Comprovou-se que a atividade do músculo apresentou diminuição no 7° dia pós-operatório para 13,7% da sua atividade inicial. Nos 30º e 60º dias após a cirurgia, observou-se a recuperação até 26,7% e 94,9% de sua atividade inicial, respectivamente. / Nowadays, a significant part of the national population presents alterations of the maxilomandibular relationship, denominated bad occlusion. The main alteration of the facial growth found in the adults is the transverse deficiency of maxillary. The correction of this deficiency is accomplished through a surgical procedure denominated. These surgeries cause a change in the muscular activity, mainly in those muscles responsible for closing mandible, in which stands out the masséter muscle. These muscular alterations last 30 days on average, where clinically it is noticed the decrease of the force or muscular potential of closing the jaw. This work evaluates, through the electromyography exam, the effects of the surgical assisted rapid maxillary expansion on the patients\' muscles masseters. Twenty patients were evaluated four times: 7 days before surgery; and 7, 30 and 60 days after surgery. The evaluation was done during the maximum voluntary isometric contraction on a cotton piece. The activity of the muscle diminished 7 days after surgery to 13, 7% of the initial activity. Nevertheless, 30 and 60 days after surgery, there was a recovery to 26, 7 and 94, 9%, respectively.

A cirurgia de terceiro molar é um dos procedimentos mais realizados na área de cirurgia e traumatologia bucomaxilofaciais. A evolução pós-cirúrgica era baseada em evidência clínica. No nosso trabalho, propusemos acompanhar a evolução pós-cirúrgica através da utilização do eletromiografia de superfície. Realizamos quatro tomadas, no pré-operatório, normalizando, e três no pós-operatório, 7, 14, 21 dias, em amostra de 30 pacientes. Observamos, no vigésimo primeiro dia, recuperação próxima da normalização no gênero feminino e ainda aquém no gênero masculino. Constatamos que a eletromiografia de superfície é sensível para controle da evolução pós-cirúrgica, demonstrando ser um método eficaz e seguro. / The third molar surgery is one of the most frenquently performed procedures in the buccal-maxillary-facial traumatology and surgery areas, whereas the post-surgery evolution has been based on the clinical evidence until now. In our study, we proposed to analyze the post-surgery clinical evolution through the use of electromyograph of surface. We have made four takes, on the presurgery, normalizing, and three on the 7, 14 and 21 postoperative days, in a sample of 30 patients. We observed, in the twentieth-first day, a recovery close to normalization on women, but men did not reach normalization. The electromyograph of surface is sensitive to the post-surgery evolution control, and is an efficient and safe method.

Background: Registering a true maximum bite force on the most commonly-used force transducers is problematic. It is often believed that this is related mainly to discomfort and the fear of breaking teeth. Objectives: The aim of the project was to compare the suitability of different bite force measuring transducers including ones which were designed to improve subject comfort. The transducers used were a traditional strain-gauge transducer with and without covering with ethylene vinyl acetate (EVA) sheets, and a newly-developed pressure transducer. Methods: Five separate studies were performed in this project. The experiments were carried out on human volunteer subjects (aged 24 to 41 years). They were all dentate with no missing anterior teeth and with no crowns on these teeth. The following procedures were used in some or all of the studies: measurement of MVBF, electrical stimulation of the masseter muscle, and EMG recording from two pairs of jaw closing muscles. Results: The highest MVBF values were recorded on the pressure transducer, mean (± S.D.) 464 N ± 224 N; followed by the strain-gauge transducer with EVA sheets, 243 ± 80 N; and last of all the strain-gauge transducer with silicone indices, 165 ± 35 N; or acrylic indices, 163 ± 82 N. Significantly higher maximum potential bite forces were predicted by twitch interpolation for the pressure transducer (730 ± 199 N) than for the strain-gauge transducer with EVA sheets, 354 ± 67 N (Paired t test, P < 0.05). Significantly higher EMGs of the masseter and anterior temporalis muscles were found to be associated with MVBFs on the pressure transducer than with MVBFs on the strain-gauge transducer with EVA sheets (Paired t test, P < 0.05). Conclusions: It is concluded that: a) the pressure transducer system and to a lesser extent the strain-gauge transducer covered with EVA sheets seemed to overcome the fear associated with biting on the hard surfaces of the strain-gauge transducer alone; b) the pressure transducer may have some multi-directional capabilities which allow for total bite forces, or at least larger parts of them, to be recorded than on a uni-directional strain-gauge transducer.

Made available in DSpace on 2014-06-11T19:28:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-12-16Bitstream added on 2014-06-13T18:35:14Z : No. of bitstreams: 1 pita_ms_me_araca.pdf: 1127223 bytes, checksum: 58ceb27b15fa47637a76872acfa04d3e (MD5) / O aumento da atividade muscular, induzido pelo apertamento dental, pode ser considerado como fator etiológico ou agravante das desordens temporomandibulares (DTMs), e essa atividade tem sido explorada de maneira direta através da eletromiografia e indiretamente pela variação da temperatura superficial cutânea sobre os músculos mastigatórios. As placas oclusais são os métodos não-invasivos mais utilizados para o tratamento das DTMs, particularmente as que afetam os músculos mastigatórios, objetivando a redução dessa hiperatividade muscular. Entretanto, há controvérsias em relação ao estabelecimento da sua espessura ideal. O objetivo do presente estudo, foi determinar o efeito da espessura de placas miorrelaxantes (3 mm e 6 mm) sobre a atividade elétrica e a temperatura dos músculos temporal anterior e masseter durante o repouso e o apertamento dental, considerando-se a condição assintomática de 20 indivíduos (10 homens e 10 mulheres) selecionados através do questionário Research Diagnostic Criteria (RDC). Os registros eletromiográficos foram realizados por meio do software “Bio EMG” (Biopack – System Bio-Research) e a temperatura das superfícies musculares realizada com o auxílio de um termômetro digital infravermelho (ICEL TD-971), com as respectivas medidas em microvolts ([V) e graus Celsius (ºC). Os resultados obtidos, analisados estatisticamente em um nível de 5% de significância (p<0,05), evidenciaram o aumento da atividade elétrica e a diminuição da temperatura dos músculos temporal anterior e masseter durante o apertamento dental em relação ao repouso. Entretanto não houve diferenças estatisticamente significativas em relação à atividade elétrica e a temperatura dos músculos mastigatórios entre as condições com e sem placa, entre o gênero masculino e feminino, bem como entre as espessuras de placas analisadas. / The increased of muscles activity, induced for dental clenching can be considered as causing or aggravating factor of temporomandibular disorders (TMD), and this activity has been exploited in a straightforward manner using electromyography and indirectly by the variation in skin surface temperature on the masticatory muscles. The occlusal splints are non-invasive methods more utilized for the treatment of TMD, particularly those from the masticatory muscles, aiming at the reduction of muscle hyperactivity. However, there are controversies regarding the establishment of their ideal thickness. The aim of this study was to determine the effect of thickness of the occlusal splints (3 mm and 6 mm) on the electrical activity and temperature of the anterior temporal and masseter muscles during dental clenching and resting, considering the asymptomatic condition of 20 individuals (10 males and 10 females) selected through the questionnaire Research Diagnostic Criteria (RDC). The electromyographic data were analyzed by the software Bio EMG (Biopack - Bio-Research System) and the surface temperatures of muscle carried out with the aid of a digital infrared thermometer (ICEL TD-971) and the corresponding measures in microvolts ([V) and degrees Celsius (ºC). The results, analyzed in a 5% of significance (p <0.05), showed increased electrical activity and lowering the temperature of the anterior temporal and masseter muscles during dental clenching in relation to the rest. However there were no statistically significant differences in relation to temperature and electrical activity of masticatory muscles between conditions with and without splints, between males and females, and between the thicknesses of occlusal splints analyzed.

Resumo: O aumento da atividade muscular, induzido pelo apertamento dental, pode ser considerado como fator etiológico ou agravante das desordens temporomandibulares (DTMs), e essa atividade tem sido explorada de maneira direta através da eletromiografia e indiretamente pela variação da temperatura superficial cutânea sobre os músculos mastigatórios. As placas oclusais são os métodos não-invasivos mais utilizados para o tratamento das DTMs, particularmente as que afetam os músculos mastigatórios, objetivando a redução dessa hiperatividade muscular. Entretanto, há controvérsias em relação ao estabelecimento da sua espessura ideal. O objetivo do presente estudo, foi determinar o efeito da espessura de placas miorrelaxantes (3 mm e 6 mm) sobre a atividade elétrica e a temperatura dos músculos temporal anterior e masseter durante o repouso e o apertamento dental, considerando-se a condição assintomática de 20 indivíduos (10 homens e 10 mulheres) selecionados através do questionário Research Diagnostic Criteria (RDC). Os registros eletromiográficos foram realizados por meio do software "Bio EMG" (Biopack - System Bio-Research) e a temperatura das superfícies musculares realizada com o auxílio de um termômetro digital infravermelho (ICEL TD-971), com as respectivas medidas em microvolts ([V) e graus Celsius (ºC). Os resultados obtidos, analisados estatisticamente em um nível de 5% de significância (p<0,05), evidenciaram o aumento da atividade elétrica e a diminuição da temperatura dos músculos temporal anterior e masseter durante o apertamento dental em relação ao repouso. Entretanto não houve diferenças estatisticamente significativas em relação à atividade elétrica e a temperatura dos músculos mastigatórios entre as condições com e sem placa, entre o gênero masculino e feminino, bem como entre as espessuras de placas analisadas. / Abstract: The increased of muscles activity, induced for dental clenching can be considered as causing or aggravating factor of temporomandibular disorders (TMD), and this activity has been exploited in a straightforward manner using electromyography and indirectly by the variation in skin surface temperature on the masticatory muscles. The occlusal splints are non-invasive methods more utilized for the treatment of TMD, particularly those from the masticatory muscles, aiming at the reduction of muscle hyperactivity. However, there are controversies regarding the establishment of their ideal thickness. The aim of this study was to determine the effect of thickness of the occlusal splints (3 mm and 6 mm) on the electrical activity and temperature of the anterior temporal and masseter muscles during dental clenching and resting, considering the asymptomatic condition of 20 individuals (10 males and 10 females) selected through the questionnaire Research Diagnostic Criteria (RDC). The electromyographic data were analyzed by the software Bio EMG (Biopack - Bio-Research System) and the surface temperatures of muscle carried out with the aid of a digital infrared thermometer (ICEL TD-971) and the corresponding measures in microvolts ([V) and degrees Celsius (ºC). The results, analyzed in a 5% of significance (p <0.05), showed increased electrical activity and lowering the temperature of the anterior temporal and masseter muscles during dental clenching in relation to the rest. However there were no statistically significant differences in relation to temperature and electrical activity of masticatory muscles between conditions with and without splints, between males and females, and between the thicknesses of occlusal splints analyzed. / Orientador: Paulo Rentato Junqueira Zuim / Coorientador: Alicio Rosalino Garcia / Banca: Karina Helga Turcio de Carvalho / Banca: Osvaldo Luiz Bezzon / Mestre