No description available.

No / In this paper we investigate the use of general topological spaces in connection with a generalised variant of membrane systems. We provide an approach which produces a fine grain description of local operations occurring simultaneously in sets of compartments of the system by restricting the interactions between objects. This restriction is given by open sets of a topology and multisets of objects associated with them, which dynamically change during the functioning of the system and which together define a notion of vicinity for the objects taking part in the interactions. / The first, the second, and the third authors were partially supported under the Royal Society International Exchanges Scheme (ref. IE110369). The second author was also partially supported by the project MuVet, Romanian National Authority for Scientific Research (CNCS – UEFISCDI) grant number PNII- ID-PCE-2011-3-0688. This work was partially completed whilst the third author was a visiting fellow at the Isaac Newton Institute for the Mathematical Sciences in the programme ‘Semantics & Syntax: A Legacy of Alan Turing’. The work of the first and the fourth author was also supported in part by the Hungarian Scientific Research Fund (OTKA), Grant no. K75952. The fourth author was supported by the European Union through the T´AMOP-4.2.2.C-11/1/KONV-2012-0001 project which is co-financed by the European Social Fund.

No description available.

This dissertation addresses the role of individual fouling mechanisms on productivity decline and solute mass transport in nanofiltration (NF) of surface waters. Fouling mechanisms as well as solute mass transport mechanisms and capabilities must be understood if NF of surface waters is to be successful. Nanofiltration of surface waters was evaluated at pilot-scale in conjunction with advanced pretreatment processes selected for minimization of nanofilter fouling, which constituted several integrated membrane systems (IMSs). Membrane fouling mechanisms of concern were precipitation, adsorption, particle plugging, and attached biological growth. Fouling was addressed by addition of acid and antiscalent for control of precipitation, addition of monochloramine for control of biological growth, microfiltration (MF) or coagulation-sedimentation-filtration (CSF) for control of particle plugging, and in-line coagulation-microfiltration (C/MF) or CSF for control of organic adsorption. Surface water solutes of concern included organic solutes, pathogens, and taste and odor compounds. Solute mass transport was addressed by evaluation of total organic carbon (TOC), Bacillus subtilis endospores, gesomin (G), 2-methlyisoborneol (MIB), and threshold odor number (TON). This evaluation included modeling to determine the role of diffusion in solute mass transport including assessment of the homogeneous solution diffusion equation. A cellulose acetate (CA) NF was less susceptible to fouling than two polyamide (PA) NFs. NF fouling was minimized by the addition of monochloramine, lower flux, lower recovery, and with the use of a coagulant-based pretreatment (C/MF or CSF). NF surface characterization showed that the low fouling CA film was less rough and less negatively charged than the PA films. Thus the theory that a more negatively charged surface would incur less adsorptive fouling, due to charge repulsion, was not observed for these tests. The rougher surface of the PA films may have increased the number of sites for adsorption and offset the charge repulsion benefits of the negatively charged surface. The addition of monochloramine significantly reduced biodegradation and integrity loss of the CA membrane. PA membranes are inherently not biologically degradable due to their chemical structure. Monochloramination reduced the rate of fouling of the PA membrane but resulted in a gradual increase in water mass transfer coefficient and a decrease in TDS rejection over time, which indicated damage and loss of integrity of the PA membrane. Based on surface characterization by X-ray Photoelectron Spectroscopy (XPS) and Fourier Transform Infrared Spectrometry (FTIR), the PA membrane degradation appeared to be chemically-based and initiated with chlorination of amide nitrogen and/or aromatic rings, which ultimately resulted in disruption of membrane chemical structures. The recommended Integrated Membrane System to control fouling of a surface water nanofiltration system is CSF monochloramine/acid/antiscalent„³monochloramine-tolerant NF. This IMS, at low flux and recovery, operated with no discernable fouling and is comparable to a groundwater nanofiltration plant with cleaning frequencies of once per six months or longer. A significant portion of the organic solutes including total organic carbon (TOC) passing through the membranes was diffusion controlled. Permeate concentration increased with increasing recovery and with decreasing flux for both PA and CA membranes. The influence was diminished for the PA membrane, due to its high rejection capabilities. Total rejection of spores used as pathogen surrogates was not achieved as spores were indigenous and high spore concentrations were used in all challenge studies; however, Integrated Membrane System spore rejection exceeded credited regulatory rejection of similar sized microorganisms by conventional treatment by several logs. Spore rejection varied by NF but only slightly by MF as size-exclusion controlled. There was no difference among spore rejection of IMS with and without in-line coagulation. Consequently, these results indicate membrane configuration (Hollow fiber>Spiral Wound) and membrane film (Composite Thin Film>CA) significantly affected spore rejection. Geosmin and methylisoborneol have molecular weights of 182 and 168 respectively, and are byproducts of algal blooms, which commonly increase taste and odor as measured by the threshold odor number (TON) in drinking water. Although these molecules are neutral and were thought to pass through NFs, challenge testing of IMS unit operations found that significant removal of TON, G and MIB was achieved by membrane processes, which was far superior to conventional processes. A CA NF consistently removed 35 to 50 percent of TON, MIB, and G, but did not achieve compliance with the TON standard of 3 units. A PA NF provided over 99 percent removal of MIB and G. Challenge tests using MIB and G indicated that size-exclusion controlled mass transfer of these compounds in NF membranes.

Yes / To solve the multi-objective mobile robot path planning in a dangerous environment with dynamic obstacles, this paper proposes a modified membraneinspired algorithm based on particle swarm optimization (mMPSO), which combines membrane systems with particle swarm optimization. In mMPSO, a dynamic double one-level membrane structure is introduced to arrange the particles with various dimensions and perform the communications between particles in different membranes; a point repair algorithm is presented to change an infeasible path into a feasible path; a smoothness algorithm is proposed to remove the redundant information of a feasible path; inspired by the idea of tightening the fishing line, a moving direction adjustment for each node of a path is introduced to enhance the algorithm performance. Extensive experiments conducted in different environments with three kinds of grid models and five kinds of obstacles show the effectiveness and practicality of mMPSO. / National Natural Science Foundation of China (61170016, 61373047), the Program for New Century Excellent Talents in University (NCET-11-0715) and SWJTU supported project (SWJTU12CX008); grant of the Romanian National Authority for Scientific Research, CNCSUEFISCDI, project number PN-II-ID-PCE- 2011-3-0688.

No

Membrane rafts (also referred as nanodomains) are membrane structures responsible for many cell processes. Their characterization is challenging because of the transparency, dynamics and small size of those structures. Moreover, high variability of cells makes their study even more complicated. In order to simplify the studies of membrane processes including the formation of those rafts often model membranes like Giant Unilamellar Vesicles (GUVs) and Supported Phospholipid Bilayers (SPBs) are used. In this Thesis new fluorescent tools for studying such membrane processed were developed, tested, or improved. Specifically, the phasor plot an approach applicable to the analysis of the fluorescence lifetime data, was theoretically and experimentally tested and afterwards applied to the characterization of the membrane nanodomains in GUVs. First, we introduced the phasor plots to the excitation state processes like solvent relaxation and Förster resonance energy transfer (FRET) in lipid vesicles. We also employed the phasor plots in protein-ligand interaction, protein folding and denaturation studies. Finally, the phasor plot analysis of FRET data in combination with Fluorescence Correlation Spectroscopy (FCS) was used in characterization of membrane nanodomains in terms of the size, mobility and...

Tensegrity-membrane systems are a class of new bar-tendon-membrane systems. Such novel systems can be treated as extensions of tensegrity structures and are generally lightweight and deployable. These two major advantages enable tensegrity-membrane systems to become one of the most promising candidates for lightweight space structures and gossamer spacecraft. In this dissertation, modeling and control of tensegrity-membrane systems is studied. A systematic method is developed to determine the equilibrium conditions of general tensegrity-membrane systems. Equilibrium conditions can be simplified when the systems are in symmetric configurations. For one-stage symmetric systems, analytical equilibrium conditions can be determined. Three mathematical models are developed to study the dynamics of tensegrity-membrane systems. Two mathematical models are developed based on the nonlinear finite element method. The other model is a control-oriented model, which is suitable for control design. Numerical analysis is conducted using these three models to study the mechanical properties of tensegrity-membrane systems. Two control strategies are developed to regulate the deployment process of tensegrity-membrane systems. The first control strategy is to deploy the system by a nonlinear adaptive controller and use a linear H∞ controller for rapid system stabilization. The second control strategy is to regulate the dynamics of tensegrity-membrane systems using a linear parameter-varying (LPV) controller during system deployment. A gridding method is employed to discretize the system operational region in order to carry out the LPV control synthesis. / Ph. D.

The interplay between lipids and membrane proteins is known to affect membrane protein topology and thus have significant effect (control) on their functions. In this PhD thesis, the influence of lipids on the membrane protein function was studied using three different membrane protein models. A monotopic membrane protein, monoglucosyldiacylglyecerol synthase (MGS) from Acholeplasma laidlawii is known to induce intracellular vesicles when expressed in Escherichia coli. The mechanism leading to this unusual phenomenon was investigated by various biochemical and biophysical techniques. The results indicated a doubling of lipid synthesis in the cell, which was triggered by the selective binding of MGS to anionic lipids. Multivariate data analysis revealed a good correlation with MGS production. Furthermore, preferential anionic lipid sequestering by MGS was shown to induce a different fatty acid modeling of E. coli membranes. The roles of specific lipid binding and the probable mechanism leading to intracellular vesicle formation were also investigated. As a second model, a MGS homolog from Synechocystis sp. PCC6803 was selected. MgdA is an integral membrane protein with multiple transmembrane helices and a unique membrane topology. The influence of different type of lipids on MgdA activity was tested with different membrane fractions of Synechocystis. Results indicated a very distinct profile compared to Acholeplasma laidlawii MGS. SQDG, an anionic lipid was found to be the species of the membrane that increased the MgdA activity 7-fold whereas two other lipids (PG and PE) had only minor effects on MgdA. Additionally, a working model of MgdA for the biosynthesis and flow of sugar lipids between Synechocystis membranes was proposed. The last model system was another integral membrane protein with a distinct structure but also a different function. The envelope stress sensor, CpxA and its interaction with E. coli membranes were studied. CpxA autophosphorylation activity was found to be positively regulated by phosphatidylethanolamine and negatively by anionic lipids. In contrast, phosphorylation of CpxR by CpxA revealed to be increased with PG but inhibited by CL. Non-bilayer lipids had a negative impact on CpxA phosphotransfer activity. Taken together, these studies provide a better understanding of the significance of the interplay of lipids and model membrane proteins discussed here.