Modified cellulase enzyme assay methods were developed to determine the potential of using enzyme activities to evaluate the decomposition of organic matter in carbon amended mine tailings systems. Cellulase assays are commonly performed in soil science applications, industrial production and bio-energy research to determine organic matter response to physical, chemical or temporal variation but, they have not been applied in a mine waste environment. Heavy metal content is considered to be a potential inhibitor to cellulase enzyme activity. Using samples collected from Greens Creek Mine in Juneau, Alaska, USA, the modified assay was evaluated to develop a method that provided reproducible results. It was determined that a sample mass of three grams is sufficient to provide consistent enzyme measurements. Each sample location was characterized by four replicates to ensure statistically representative data. Matrix interferences were determined to be inconsequential in this system. Despite the low organic content amended to the tailings, heavy metal content and potentially low enzyme activity, the modified enzyme assay method provided reproducible enzyme measurements. Following the development of the cellulase assays, spatial and temporal variations in cellulase enzymes were investigated from carbon amended tailings samples collected at the Greens Creek Mine. Six test cells, containing a mixture of tailings and a combination of peat, spent brewery grain and/or municipal biosolids were sampled in the fall of 2005, 2007 and 2009. Exo-(1,4)-β-D-glucanase (EC 3.2.1.91), Endo-(1,4)-β-D-glucanase (EC 3.2.1.4) and β-glucosidase (EC 3.2.1.21) enzymes were assayed from core sections at five different depths. Enzyme activities were compared to sulfate reducing and acid producing bacterial enumerations, sulfide trends and carbon content. General trends were consistent between enzyme activity and SRB enumerations. The range of total carbon values fell between 3 and 5 wt % in each test cell while the average inorganic carbon content was 3.5 wt %. The range of organic carbon content was between 0.2 and 1.2 wt %. Total, inorganic and organic carbon values were more characteristic of test cell carbon distribution. Cellulase enzyme assays provide valuable information regarding the degradation of cellulose and hemi-cellulose. This study demonstrates that enzymes can be monitored in a tailings environment and that enzyme assays conducted for monitoring purposes may be a useful practice to indicate the sustained or declining performance of organic matter in a carbon amended remedial system.

Smaltjärnen tailings repository located in Yxsjöberg, Sweden, attracts researchers with questions regarding characterization and potential re-mining. This thesis continuous geophysical characterization done by researchers from the Exploration Geophysics department at Luleå University of Technology but using new data from 2019. Geophysical methods used were self potential (SP), direct current resistivity (DCR), induced polarization (IP), and ground-penetrating radar (GPR). SP data were collected using a fixed base procedure and equipment from EMIT. Data were processed in MATLAB and presented in Oasis Montaj/ Geosoft software, yielding results difficult to interpret. Similar pattern was seen in previous investigations from 2016. However, some discrepancies were noticed, and more work is needed in order to validate these data. Therefore, data is presented without any interpretation. RES2DINV inversion software by Geotomo Software (now maintained by Aarhus GeoSoftware) was used for inversion of DCR data to produce four 2D resistivity sections, and the 3D resistivity model was made by Jingyu Gao with his software. DCR data were acquired by using Terrameter LS by ABEM and measuring using a roll-along procedure and dipole-dipole configuration. Results show consistency between vertical variations at profile crossings from different profiles. Three layers are indicated from results, interpreted to contain mine tailings and quaternary deposits, at some locations interpreted to be water-saturated, and bedrock. No IP effect is seen at Smaltjärnen. GPR data were processed in GPRSoft® PRO produced by Geoscanners to understand internal structures and water table, by using zero-offset surveying with 250 MHz antenna from Malå Geoscience and 300 MHz antenna from Geoscanners. Since the tailings of Smaltjärnen consists of very thin layers, the results are complex to interpret. Hyperbolas and layers, along with other more uncertain patterns, are seen in radargrams, and further research is needed to fully understand the images.

South Africa has an array of mining commodities which all play an integral role in our everyday surroundings, income, and most importantly, in the economy of the country. These mining activities also produce vast amounts of discard material, better known as tailings material, which is stored in different ways after extraction has taken place. Usually, storage entails the construction of tailings storage facilities, normal discard or tailings dumps. The upper surfaces of these anthropogenic structures are usually unstable and are, in most cases, characterised by different forms of erosion. This can be due to the chemical and physical properties of the materials of which they are constructed, but mainly due to unstable construction geomorphology, steep slopes, which leads to poor water run-off management and subsequent instability. Therefore, these structures need to be actively managed in order to increase and maintain their stability. Grass establishment, as a stabilisation technique, is the most effective out of all of the techniques, but there are certain constraints regarding this method (Titshall et al. 2013). The most costly constraint is nutrient supplementation during aftercare phases. In order to minimize this cost, new and innovative technologies need to be explored, and trialled. The contribution of soil biological processes in this regard was assessed, in order to minimise anthropogenic inputs. These biological processes refer to the fixation of atmospheric nitrogen by nodular root bacteria that grow on a group of plants referred to as legumes. These bacteria, also known as rhizobia, live in a symbiotic relationship with the host plant where they receive energy in the form of nutrients by trading nitrogen, which is an essential plant nutrient. Nine different tailings materials from different commodities available from South African Mines were selected. For a control medium, a well-drained soil type with an apedel structure and a clay content of approximately 6% was selected in order to promote optimal natural growth. These materials were chemically and physically analysed in order to develop a more holistic understanding on a micro scale level, as well as to ascertain possible constraints in this regard. Pot trials were selected as the experimental method in order to apply more specific control over root growth, plant development and growing conditions. The experimental data were collected over one growing season for both live forms. For this study, seven legume species were selected for establishment in the tailings materials in order to investigate their establishment potential in the growth mediums and their ability to fixate nitrogen. Based on the data, specific species were identified as viable options to include in future tailings amelioration projects; it can be assumed that the nitrogen produced by these species will be available in the growth medium for uptake by neighbouring plants that lack this biological function. These plants will also play a vital role in the long-term sustainable development of vegetation in the anthropogenic growth mediums. Sericea lespedeza had the highest enrichment ability during this study. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2015

South Africa has an array of mining commodities which all play an integral role in our everyday surroundings, income, and most importantly, in the economy of the country. These mining activities also produce vast amounts of discard material, better known as tailings material, which is stored in different ways after extraction has taken place. Usually, storage entails the construction of tailings storage facilities, normal discard or tailings dumps. The upper surfaces of these anthropogenic structures are usually unstable and are, in most cases, characterised by different forms of erosion. This can be due to the chemical and physical properties of the materials of which they are constructed, but mainly due to unstable construction geomorphology, steep slopes, which leads to poor water run-off management and subsequent instability. Therefore, these structures need to be actively managed in order to increase and maintain their stability. Grass establishment, as a stabilisation technique, is the most effective out of all of the techniques, but there are certain constraints regarding this method (Titshall et al. 2013). The most costly constraint is nutrient supplementation during aftercare phases. In order to minimize this cost, new and innovative technologies need to be explored, and trialled. The contribution of soil biological processes in this regard was assessed, in order to minimise anthropogenic inputs. These biological processes refer to the fixation of atmospheric nitrogen by nodular root bacteria that grow on a group of plants referred to as legumes. These bacteria, also known as rhizobia, live in a symbiotic relationship with the host plant where they receive energy in the form of nutrients by trading nitrogen, which is an essential plant nutrient. Nine different tailings materials from different commodities available from South African Mines were selected. For a control medium, a well-drained soil type with an apedel structure and a clay content of approximately 6% was selected in order to promote optimal natural growth. These materials were chemically and physically analysed in order to develop a more holistic understanding on a micro scale level, as well as to ascertain possible constraints in this regard. Pot trials were selected as the experimental method in order to apply more specific control over root growth, plant development and growing conditions. The experimental data were collected over one growing season for both live forms. For this study, seven legume species were selected for establishment in the tailings materials in order to investigate their establishment potential in the growth mediums and their ability to fixate nitrogen. Based on the data, specific species were identified as viable options to include in future tailings amelioration projects; it can be assumed that the nitrogen produced by these species will be available in the growth medium for uptake by neighbouring plants that lack this biological function. These plants will also play a vital role in the long-term sustainable development of vegetation in the anthropogenic growth mediums. Sericea lespedeza had the highest enrichment ability during this study. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2015

Phytostabilization offers a less expensive alternative to traditional cap and plant methods for containing metalliferous mine tailings to prevent wind erosion and contamination of nearby communities and the environment. However, plant establishment during phytostabilization of pyritic legacy mine tailings in semiarid regions is challenging due to particularly extreme conditions including low pH, low organic carbon, low nutrients, and high toxic metal(loid) concentrations. Microorganisms drive major biogeochemical cycles in soils, however, the roles microorganisms play at the root – soil interface during phytostabilization, particularly in relation to plant health and metal immobilization, are not yet fully understood. The aims of this dissertation are to focus on bacterial communities associated with the roots of buffalo grass used in the phytostabilization of pyritic metalliferous mine tailings to: i) characterize bacterial diversity and communities of rhizosphere and bulk substrate, ii) delineate associations between rhizoplane bacterial colonization patterns and environmental and plant status parameters, and iii) develop an in situ method to visually assess associations between roots, bacteria, and metals. Key findings indicate that after addition of a compost amendment to alleviate the plant-growth inhibiting characteristics of mine tailings, rhizosphere and bulk substrate contain a diverse, plant-growth supporting bacterial community. As substrate re-acidifies due to compost erosion, an emergence of an iron (Fe)- and sulfur (S)-oxidizer and Fe-reducer dominated, less diverse community develops in the bulk and rhizosphere substrate, thus posing a threat to successful plant establishment. However, even at low pH, some plant-growth-promoting bacteria are still evident in the rhizosphere. On the rhizoplane (root surface), the relative abundance of metabolically active bacteria was positively correlated with plant health, verifying the strong association between plant health and bacteria. Furthermore, pH showed a strong association with the relative abundance of Alphaproteobacteria and Gammaproteobacteria on the rhizoplane. In relation to microbe-metal interactions on the root surface, results showed that Actinobacteria and Alphaproteobacteria colocalized with Fe-plaque and arsenic (As) contaminant on the root surface, indicating their potential role in adsorbing or cycling of these metal(loid)s. Developing a more thorough understanding of bacteria-root-metal interactions in relation to plant health and metal immobilization can help to improve phytostabilization efforts and success.

Student Number : 0418755N - MSc (Eng) dissertation - School of Civil and Environmental Engineering - Faculty of Engineering and the Built Environment / Environmental impacts from tailings impoundments differ according to their mineral constituents. Erosion is one of the processes that aggravate the environmental impacts from tailings due to the transportation of particles, and knowing the susceptibility mechanisms of those tailings particles for erosion will provide understanding of how to prevent impacts arising from erosion. Laboratory pinhole erosion tests were used to determine the susceptibility of tailings particles to erosion. Compacted tailings samples were used, as compaction is an important parameter of erosion susceptibility. The study entails investigation of factors that affect erosion from the slopes of tailings deposits in order to evaluate mitigation measures. The results could help to provide more effective methods to reduce gully formation and enhance environmental protection. It is advisable to prevent environmental impacts at the source, before they become detrimental and costly to mitigate.

<p>Mine tailings can be rich in sulphide minerals and may form acid mine drainage (AMD) through reaction with atmospheric oxygen and water. AMD contains elevated levels of metals and arsenic (As) that could be harmful to animals and plants. An oxygen-consuming layer of organic material and plants on top of water-covered tailings would probably reduce oxygen penetration into the tailings and thus reduce the formation of AMD. However, wetland plants have the ability to release oxygen through the roots and could thereby increase the solubility of metals and As. These elements are released into the drainage water, taken up and accumulated in the plant roots, or translocated to the shoots. </p><p>The aim was to examine the effects of plant establishment on water-covered mine tailings by answering following questions: A) Is plant establishment on water-covered mine tailings possible? B) What are the metal and As uptake and translocation properties of these plants? C) How do plants affect metal and As release from mine tailings, and which are the mechanisms involved?</p><p><i>Carex rostrata Stokes, Eriophorum angustifolium</i> Honck., <i>E. scheuchzeri</i> Hoppe, <i>Phragmites australis</i> (Cav.) Steud., <i>Salix phylicifolia</i> L. and <i>S. borealis</i> Fr. were used as test plants. Influences of plants on the release of As, Cd, Cu, Pb, Zn and in some cases Fe in the drainage water, and plant element uptake were studied in greenhouse experiments and in the field. </p><p>The results obtained demonstrate that plant establishment are possible on water-covered unweathered mine tailings, and a suitable amendment was found to be sewage sludge. On acidic, weathered tailings, a pH increasing substance such as ashes should be added to improve plant establishment. The metal and As concentrations of the plant tissue were found to be generally higher in roots than in shoots. The uptake was dependent on the metal and As concentrations of the tailings and the release of organic acids from plant roots may have influenced the uptake. The metal release from tailings into the drainage water caused by<i> E. angustifolium </i>was found to depend greatly on the age and chemical properties of the tailings. However, no effects of <i>E. angustifolium </i>on As release was found. Water from old sulphide-, metal- and As-rich tailings with low buffering capacity were positively affected by <i>E. angustifolium </i>by causing higher pH and lower metal concentrations. In tailings with relatively low sulphide, metal and As contents combined with a low buffering capacity, plants had the opposite impact, i.e. a reduction in pH and elevated metal levels of the drainage water. The total release of metal and As from the tailings, i.e. drainage water together with the contents in shoots and roots, was found to be similar for <i>C. rostrata</i>, <i>E. angustifolium </i>and <i>P. australis</i>, except for Fe and As, where the release was highest for <i>P. australis</i>. The differences in metal and As release from mine tailings were mainly found to be due to the release of O₂from the roots, which changes the redox potential. Release of organic acids from the roots slightly decreased the pH, although did not have any particular influence on the release of metal and As. </p><p>In conclusion, as shown here, phytostabilisation may be a successful technique for remediation of mine tailings with high element and sulphide levels, and low buffering capacity.</p>

Mine tailings can be rich in sulphide minerals and may form acid mine drainage (AMD) through reaction with atmospheric oxygen and water. AMD contains elevated levels of metals and arsenic (As) that could be harmful to animals and plants. An oxygen-consuming layer of organic material and plants on top of water-covered tailings would probably reduce oxygen penetration into the tailings and thus reduce the formation of AMD. However, wetland plants have the ability to release oxygen through the roots and could thereby increase the solubility of metals and As. These elements are released into the drainage water, taken up and accumulated in the plant roots, or translocated to the shoots. The aim was to examine the effects of plant establishment on water-covered mine tailings by answering following questions: A) Is plant establishment on water-covered mine tailings possible? B) What are the metal and As uptake and translocation properties of these plants? C) How do plants affect metal and As release from mine tailings, and which are the mechanisms involved? Carex rostrata Stokes, Eriophorum angustifolium Honck., E. scheuchzeri Hoppe, Phragmites australis (Cav.) Steud., Salix phylicifolia L. and S. borealis Fr. were used as test plants. Influences of plants on the release of As, Cd, Cu, Pb, Zn and in some cases Fe in the drainage water, and plant element uptake were studied in greenhouse experiments and in the field. The results obtained demonstrate that plant establishment are possible on water-covered unweathered mine tailings, and a suitable amendment was found to be sewage sludge. On acidic, weathered tailings, a pH increasing substance such as ashes should be added to improve plant establishment. The metal and As concentrations of the plant tissue were found to be generally higher in roots than in shoots. The uptake was dependent on the metal and As concentrations of the tailings and the release of organic acids from plant roots may have influenced the uptake. The metal release from tailings into the drainage water caused by E. angustifolium was found to depend greatly on the age and chemical properties of the tailings. However, no effects of E. angustifolium on As release was found. Water from old sulphide-, metal- and As-rich tailings with low buffering capacity were positively affected by E. angustifolium by causing higher pH and lower metal concentrations. In tailings with relatively low sulphide, metal and As contents combined with a low buffering capacity, plants had the opposite impact, i.e. a reduction in pH and elevated metal levels of the drainage water. The total release of metal and As from the tailings, i.e. drainage water together with the contents in shoots and roots, was found to be similar for C. rostrata, E. angustifolium and P. australis, except for Fe and As, where the release was highest for P. australis. The differences in metal and As release from mine tailings were mainly found to be due to the release of O2 from the roots, which changes the redox potential. Release of organic acids from the roots slightly decreased the pH, although did not have any particular influence on the release of metal and As. In conclusion, as shown here, phytostabilisation may be a successful technique for remediation of mine tailings with high element and sulphide levels, and low buffering capacity.

Biogeochemical reactions and vadose zone transport, in particular gas phase transport, are inherently coupled processes. To explore feedback mechanisms between these processes in a quantitative manner, multicomponent gas diffusion and advection are implemented into an existing reactive transport model that includes a full suite of geochemical reactions. Multicomponent gas diffusion is described based on the Dusty Gas Model, which provides the most generally applicable description for gas diffusion. Gas advection is described by Darcy's Law, which in the current formulation, is directly substituted into the transport equations. The model is used to investigate the interactions between geochemical reactions and transport processes with an emphasis to quantify reaction-induced gas migration in the vadose zone. Simulations of pyrite oxidation in mine tailings, gas attenuation in partially saturated landfill soil covers, and methane production and oxidation in aquifers contaminated by organic compounds demonstrate how biogeochemical reactions drive diffusive and advective transport of reactive and non-reactive gases. Pyrite oxidation in mine tailings causes a pressure reduction in the reaction zone and drives advective gas flow into the sediment column, enhancing the oxidation process. Release of carbon dioxide by carbonate mineral dissolution partly offsets pressure reduction, and illustrates the role of water-rock interaction on gas transport. Microbially mediated methane oxidation in landfill covers reduces the existing upward pressure gradient, thereby decreasing the contribution of advective methane emissions to the atmosphere and enhancing the net flux of atmospheric oxygen into the soil column. At an oil spill site, both generation of CH4 in the methanogenic zone and oxidation of CH4 in the methanotrophic zone contribute to drive advective and diffusive fluxes. The model confirmed that non-reactive gases tend to accumulate in zones of gas consumption and become depleted in zones of gas production. In most cases, the model was able to quantify existing conceptual models, but also proved useful to identify data gaps, sensitivity, and inconsistencies in conceptual models. The formulation of the model is general and can be applied to other vadose zone systems in which reaction-induced gas transport is of importance.

In this thesis, column experiments were conducted in order to determine the effect of irrigation with local groundwater on mobilization of lead and zinc in 50 years old sulfate-acid mine tailings. In addition, the influence of soluble oxalic acid, a common rhizosphere organic acid, was assessed by varying its concentration across an environmentally relevant range. In general, metal contaminant dissolution was not affected by the presence of oxalic acid. In both tailings, Zn mobilization was higher than Pb suggesting the presence of more kinetically labile Zn phases, regardless of the treatment used. Lead mobilization was also low because effluent solutions were near to equilibrium conditions with respect to gypsum, preventing Pb dissolution from Pb-sulfate minerals. Geochemical modeling also indicated that lead release was controlled by anglesite and plumbojarosite dissolution. Zinc release appears to be controlled by Zn-talc and goslarite.