Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2014-11-24T13:11:26Z No. of bitstreams: 2 Dissertação - Ana Paula de Toledo Vieira - 2012.pdf: 10699833 bytes, checksum: 2e25915716db787ecacbd31491c5309a (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2014-11-24T14:02:12Z (GMT) No. of bitstreams: 2 Dissertação - Ana Paula de Toledo Vieira - 2012.pdf: 10699833 bytes, checksum: 2e25915716db787ecacbd31491c5309a (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2014-11-24T14:02:12Z (GMT). No. of bitstreams: 2 Dissertação - Ana Paula de Toledo Vieira - 2012.pdf: 10699833 bytes, checksum: 2e25915716db787ecacbd31491c5309a (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2012-02-27 / Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG / Nematoda Lagochilascaris minor is the etiologic agent of lagochilascaríase in human beings. Until today, no one knows the natural host of this helminth and Brazil registers about 90% of the cases of lagochilascaríase world-wide human being. The life cycle of L. minor was described using an experimental model in mice and domestic cat, behaving as an intermediate host and definitive host respectively. The objective of this study was to evaluate the histology of the adult males of this helminth worms, using light microscopy. These specimens were removed after necropsy of cats kept in their cages cataloged in male and female worms, and the adult male specimens were fixed in formalin solution 10 % hot, AFA and 4% paraformaldehyde in sodium cacodylate buffer 0 1 M, pH 7.2. After the different fixations, the samples were dehydrated in a gradual series of ethanol in different concentrations of alcohol levels, and included in historesin cut with 3μm –thick microtome, with horizontal and transverse serial sections. The sections were stained with Hematoxylin & Eosin (HE), Carmin, Giemsa and Toluidine Blue. The best fixation was achieved with the use of AFA and 4% paraformaldehyde in 0.1 M sodium cacodylate buffer pH 7.2 and the best staining was obtained from the use of the dye HE, which showed histological structures of adult male worms L. minor loop as testicular, seminal vesicle, ejaculatory duct, sperm and spicule. It was also observed structures of the digestive system, muscular system, cuticle, wing side and lateral line. The sections were photomicrographed using photomicroscope with the help of the program Motic Images Plus 2.0, selected and analyzed. / O nematoda Lagochilascaris minor é o agente etiológico da lagochilascaríase em humanos. Até hoje, não se conhece o hospedeiro natural deste helminto e o Brasil registra cerca de 90% dos casos da lagochilascaríase humana mundial. O ciclo evolutivo de L. minor foi descrito utilizando um modelo experimental com camundongo e gato doméstico, comportando-se como hospedeiro intermediário e hospedeiro definitivo, respectivamente. O objetivo deste trabalho foi avaliar a histologia dos vermes adultos machos deste helminto, utilizando microscopia de luz. Estes exemplares foram retirados após necropsia de gatos mantidos em biotério, catalogados em vermes machos e fêmeas, sendo que os exemplares machos adultos foram fixados em solução de Formalina a 10% a quente, AFA e Paraformaldeído a 4% em tampão cacodilato de sódio 0,1 M pH 7,2. Após as diferentes fixações, as amostras foram desidratadas em uma série gradual de etanol em diferentes concentrações de teores alcoólicos, incluídas em historesina e cortadas com 3μm de espessura em micrótomo, com secções seriadas horizontais e transversais. Os cortes foram corados com Hematoxilina & Eosina (HE), Carmim, Giemsa e Azul de Toluidina. A melhores fixações foram alcançadas com o uso de AFA e Paraformaldeído a 4% em tampão cacodilato sódio 0,1 M pH 7,2 e a melhor coloração obteve-se a partir da utilização do corante HE, que evidenciou estruturas histológicas dos vermes adultos machos de L. minor como alça testicular, vesícula seminal, ducto ejaculatório, espermatozoides e espículo. Observou-se também estruturas do sistema digestório, sistema muscular, cutícula, asa lateral e linha lateral. As imagens dos cortes foram realizadas utilizando um fotomicroscópio com o auxílio do programa Motic Images Plus 2.0, selecionadas e analisadas.

Made available in DSpace on 2014-07-29T15:30:43Z (GMT). No. of bitstreams: 1 AlessandraPadua2006.pdf: 748062 bytes, checksum: 6119b87ba9ccd000536ca1e719e321dc (MD5) Previous issue date: 2006-03-31 / A lagochilascaríase é uma infecção causada por Lagochilascaris minor, parasito causador de lesões debilitantes da região cervical de humanos, que apesar de não constituir um problema de saúde pública, é emergente devido ao crescente número de casos no Brasil. Camundongos C57BL/6 e B10.A foram inoculados por via oral com 2000 ovos viáveis de L. minor com o intuito de analisar aspectos parasitológicos, morfológicos, histopatológicos e sobrevida. Camundongos C57BL/6 apresentaram maior número de nódulos (machos, 330; fêmeas, 210) que os camundongos B10.A (machos, 225; fêmeas, 110) aos 90 dias após infecção. Larvas (L3/L4) e vermes adultos foram encontrados nos nódulos de ambas as linhagens, mas não houve diferença quanto ao tamanho e largura das mesmas. Infiltrados inflamatórios focais de menor intensidade predominaram no fígado de camundongos C57BL/6 aos 15 dias de infecção, enquanto no mesmo período, em camundongos B10.A houve presença em maior intensidade de infiltrados inflamatórios focais mais bem formados. Camundongos C57BL/6 apresentaram infiltrado inflamatório difuso pulmonar, do início ao final do período avaliado, de maior gravidade que os apresentados por animais B10.A, principalmente após 120 dias de infecção. Em ambas as linhagens observamos no pulmão granulomas cisticos contendo larvas L3 integras ou em início de degeneração, dos 45 aos 210 dias de infecção; entretanto, camundongos B10.A apresentaram formação precoce (60 dias após infecção) de infiltrados inflamatórios focais sem presença de larvas L3 no pulmão, quando comparados aos animais da linhagem C57BL/6 (120 dias após infecção). A análise de sobrevida durante 360 dias de infecção em ambas as linhagens, demonstrou que camundongos B10.A (44%) sobreviveram um pouco mais do que os camundongos C57BL/6 (39%), sendo esta diferença estatisticamente significativa. Estes dados evidenciam que animais B10.A são pouco mais resistentes à infecção por L. minor que camundongos C57BL/6, mas esta diferença é estatisticamente significativa; e que animais das duas linhagens podem atuar como hospedeiro definitivo e intermediário na infecção experimental.

This thesis examines the history and origins of Beethoven's E minor Piano Sonata and examines the possibility of the programmatic conception of the work. Dedicated to Beethoven's friend Count Moritz Lichnowsky, the sonata may have been inspired by the Count's illicit affair with his future wife, the singer and actress Josefa Stummer. Providing a thorough Schenkerian analysis of both movements, the inner harmonic structure of the composition is revealed and explained. The author also investigates and details the unpublished original analyses of the composition by Heinrich Schenker, Erika Elias, and Hans Weisse. Both English and German language sources are incorporated into a comprehensive examination of Beethoven's Piano Sonata, op. 90.

Most studies of the Mass in B Minor deal with the history of the work, its reception history, primary sources, performance practice issues, rhetoric, and even theological and numerical symbolism. However, little research focuses on an in-depth analysis of the music itself. Of the few analytical studies undertaken, to date only a limited number attempt to explain Bach's use of parody technique or unity in the whole composition. This thesis focuses on understanding three primary concerns in regards to the Mass in B minor: to comprehend how preexistent material was adapted to the context of the Mass, how this material functions in the network of the entire composition, and how unity is achieved by means of large-scale voice leading. The results of this study not only provide new information about this monument of Western music, but also provide insight to the deep sense of large-scale structure in Bach's work.

The purpose if this study is to make an analysis of the structural elements and stylistic characteristics in the Concerto in A Minor for Piano by the nineteenth century German composer, Robert Schumann. These elements include the composer's treatment of melody, rhythm, form, and piano idiom. This problem has been limited to a stylistic analysis of Schumann's only concerto in A minor for piano and orchestra, Op. 54. Its purpose is to make an analysis of the structural and stylistic elements in the Concerto. These include the composer's treatment of melody, rhythm, form and the piano idiom. Since the matter of harmony is not of primary importance in this work, it is not discussed here. The present study does not include a consideration of the orchestral score and its relationship to the piano; however mention of it is made in the chapter on piano idiom.

abstract: The core element of this research paper is an analysis of the B-flat minor prelude and fugue BWV 867. The author’s analysis is then compared with the main contributors to Bach’s analytical studies. An understanding of the work’s structure, together with its motivic and harmonic details, help the performer develop an interpretive approach to the work. Significant Bach scholars, including David Ledbetter and Peter Williams, are used as the source for the additional works to be studied. These scholars also mention close associates of Bach who offer additional insight into his music: Kirnberger and Weiss. The paper includes a brief discussion of the opening chorus fugue BWV 64, instrumental prelude to the cantata BWV 106, chorale prelude BWV 721, and Tombeau sur la Mort de Mr Comte de Logy by Weiss, as they relate to the B-flat minor prelude and fugue. In addition, the analysis provides materials on how the elements of the work relate to the Doctrine of Affections. From the B-flat minor prelude and fugue, the harmonic progressions and figuration are examined from the point of view of the Doctrine. The research also examines the fugue subject, with its unusual leap of a minor 9th, to its structural connection to the opening chorus fugue of BWV 64, and its ties to the Doctrine. Through the analysis of the B-flat minor prelude and fugue and a comparison to works by Bach that are stylistically connected to this work, the author offers insights into the music and its relationship to works that have a sacred text association. / Dissertation/Thesis / Doctoral Dissertation Music 2020

Seit 1983 begründet die Publikationsreihe "Graph Minors" von N. Robertson und P.D. Seymour im Wesentlichen die Minorentheorie mit mächtigen Hilfsmitteln wie der Baumzerlegung und weitreichenden Resultaten wie dem Minorensatz. Für gerichtete Graphen existiert allerdings noch keine einheitliche Minorentheorie und verschiedene Ansätze werden in dieser Arbeit systematisiert. Einige gerichtete Versionen der Baumzerlegung (gerichtete Baumzerlegung nach B. Reed, arboreale, D- und DAG-Zerlegung) werden unter einheitlichen Aspekten untersucht. Die D-Weite ist dabei besonders vielversprechend. Enge Verbindungen zu zwei gerichteten Räuber-und-Gendarmen-Spielen werden unter analogen Aspekten betrachtet und sind wichtige Hilfsmittel. Der zentrale Begriff des Minoren ist im Wesentlichen für ungerichtete Graphen definiert und eine gerichtete Version wirft einige Probleme auf, welche untersucht werden. In \"Directed Tree-Width\" schlugen T. Johnson, N. Robertson, P.D. Seymour und R. Thomas 2001 einen Kompromiss vor. Durch Einschränkung der möglichen Kontraktionen soll der gewonnen Minorenbegriff mit einigen fundamentalen Anforderungen vereinbar sein und trotzdem ein mächtiges Werkzeug darstellen. Dieser Ansatz wird mit einer Anforderungsliste systematisch verfolgt und schrittweise Einschränkungen betrachtet. Die gerichtete Version topologischer Minoren ist dabei besonders vielversprechend. Die Minorentheorie gerichteter Graphen wird auf reduzible Flussgraphen angewandt. Wesentliche Resultate sind Konstruktionen arborealer und D-Zerlegungen mit Weite <2, sowie Gegenbeispiele für die Beschränktheit der DAG-Weite. Analoge Resultate folgen für die jeweiligen gerichteten Räuber-und-Gendarmen-Spiele.

Seit 1983 begründet die Publikationsreihe "Graph Minors" von N. Robertson und P.D. Seymour im Wesentlichen die Minorentheorie mit mächtigen Hilfsmitteln wie der Baumzerlegung und weitreichenden Resultaten wie dem Minorensatz. Für gerichtete Graphen existiert allerdings noch keine einheitliche Minorentheorie und verschiedene Ansätze werden in dieser Arbeit systematisiert. Einige gerichtete Versionen der Baumzerlegung (gerichtete Baumzerlegung nach B. Reed, arboreale, D- und DAG-Zerlegung) werden unter einheitlichen Aspekten untersucht. Die D-Weite ist dabei besonders vielversprechend. Enge Verbindungen zu zwei gerichteten Räuber-und-Gendarmen-Spielen werden unter analogen Aspekten betrachtet und sind wichtige Hilfsmittel. Der zentrale Begriff des Minoren ist im Wesentlichen für ungerichtete Graphen definiert und eine gerichtete Version wirft einige Probleme auf, welche untersucht werden. In \"Directed Tree-Width\" schlugen T. Johnson, N. Robertson, P.D. Seymour und R. Thomas 2001 einen Kompromiss vor. Durch Einschränkung der möglichen Kontraktionen soll der gewonnen Minorenbegriff mit einigen fundamentalen Anforderungen vereinbar sein und trotzdem ein mächtiges Werkzeug darstellen. Dieser Ansatz wird mit einer Anforderungsliste systematisch verfolgt und schrittweise Einschränkungen betrachtet. Die gerichtete Version topologischer Minoren ist dabei besonders vielversprechend. Die Minorentheorie gerichteter Graphen wird auf reduzible Flussgraphen angewandt. Wesentliche Resultate sind Konstruktionen arborealer und D-Zerlegungen mit Weite <2, sowie Gegenbeispiele für die Beschränktheit der DAG-Weite. Analoge Resultate folgen für die jeweiligen gerichteten Räuber-und-Gendarmen-Spiele.:1 Einleitung 1.1 Grundbegriffe der Graphentheorie 1.2 Reduzibilität 2 Über Minoren von Graphen 2.1 Minoren von Graphen 2.2 Topologische Minoren von Graphen 2.3 Baumzerlegung und Baumweite tw(G) 2.4 Wegzerlegung und Wegbreite pw(G) 2.5 Räuber-und-Gendarmen-Spiele auf Graphen 2.6 Resultate und Anwendungen 3 Über Minoren von Digraphen 3.1 Übertragung der Minorenrelation auf Digraphen 3.2 Hindernisse bei der De?nition einer gerichteten Baumweite 3.3 Arboreale Weite dtw(D) 3.3.1 Arboreale Weite und Räuber-und-Gendarmen-Spiele 3.3.2 Resultate und Anwendungen 3.4 Gerichtete Baumweite dtwR(D) 3.5 D-Weite dw(D) 3.6 DAG-Weite dgw(D) 3.6.1 DAG-Weite und Räuber-und-Gendarmen-Spiele 3.6.2 Resultate und Anwendungen 3.7 Räuber-und-Gendarmen-Spiele auf Digraphen 3.8 Eingeschränkte Minorenrelation für Digraphen 3.8.1 Die Teilgraphenrelation für Digraphen 3.8.2 Die topologische Minorenrelation für Digraphen 3.8.3 Die Minorenrelation auf Digraphen nach JRST 3.8.4 Eingeschränkte Minorenrelationen 4 Verbindungen zwischen Reduzibilität und Minoren von Digraphen 4.1 Reduzibilität und initiale Wurzeldigraphen 4.2 Charakterisierung der Reduzibilität durch eingeschränkte Minoren 4.3 Resultate der Minorentheorie für reduzible initiale Wurzeldigraphen 5 Zusammenfassung und Ausblick Literaturverzeichnis Abbildungsverzeichnis

This research explored fate of organic contaminants in aquatic plant systems through (i) experimental development of relationships to describe sorption, uptake and enzymatic processing of contaminants by plants and inhibition of aquatic plants by contaminants and (ii) incorporation of experimental relationships into a conceptual model which describes contaminant fate in aquatic plant systems. This study focused on interactions of aquatic plants L. minor and M. aquaticum with halogenated phenols. 2,4,5-trichlorophenol (2,4,5-TCP) and 2,4-dichlorophenol (2,4-DCP) are precursors for the highly toxic and heavily applied herbicides 2,4,5-T and 2,4-D and were examined in detail. Chlorophenols are generally resistant to microbial degradation, a property which may limit microbial remediation options as effective alternatives for clean up of contaminated sites. Relationships for fundamental interactions between plants and contaminants that dictate uptake, enzymatic processing and sequestration of contaminants by aquatic plants were established. An assay which quantified production of oxygen by plants was developed to quantify plant metabolic activity and inhibition. Uptake of chlorinated phenols depended on plant activity and aqueous phase concentration of contaminant in the protonated form. Therefore, plant activity, contaminant pKa and media pH were established as critical parameters controlling rate of contaminant uptake. A conceptual model was developed which incorporated plant activity and inhibition into a mathematical description of uptake of organic contaminants by aquatic plants. The conceptual model was parameterized using experimental data delineating effect of plant activity, inhibition and speciation on contaminant uptake and the model was verified using independently gathered data. Experimentation with radio-labeled chlorinated phenols established that contaminants were sequestered internal to plants by plant enzymatic processing. 19F NMR was established as a technique to quantify transformation and conjugation products internal to plants and contaminant assimilation by plants and demonstrated that multiple metabolites containing the parent compound were present and quantifiable internal to plants. Finally, fate of plant-sequestered contaminants in an anaerobic bioassay was examined using Desulfitobacterium sp. strain Viet1. The results of this study address the role of aquatic plants in sequestration of contaminants in surface waters that indicate the potential and limitations of use of aquatic plants in natural and engineered treatment systems.

The purpose of the following study is to make an analysis of the structural elements and stylistic characteristics of the Sonata in A Minor for violin and piano by David Stanley Smith. This analysis will include the composer's treatment of form, harmony, melody and tonality, rhythm, intellectual and emotional content, and mediums of expression.