by Nellie Yuk Fei Chung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 111-120). / Abstract also in Chinese. / Abstract --- p.i / List of Abbreviations --- p.iii / Acknowledgements --- p.iv / Publication of this study --- p.vi / Table of Contents --- p.vii / Chapter Chapter1: --- Introduction --- p.1 / Chapter 1.1 --- Multiple Myeloma --- p.2 / Chapter 1.2 --- The Problem --- p.2 / Chapter Chapter2: --- Literature Review --- p.5 / Chapter 2.1 --- Molecular Genetics of Multiple Myeloma --- p.6 / Chapter 2.1.1 --- Cytogenetics --- p.6 / Chapter 2.2 --- Alterations of Proto-Oncogenes --- p.9 / Chapter 2.2.1 --- c-myc --- p.9 / Chapter 2.2.2 --- Ras --- p.10 / Chapter 2.2.3 --- Bcl-2 and Related Protein --- p.10 / Chapter 2.3 --- Alteration of Tumor-Suppressor genes --- p.11 / Chapter 2.3.1 --- p53 Gene Mutations --- p.11 / Chapter 2.3.2 --- Retinoblastoma (Rb) Gene --- p.11 / Chapter 2.3.3 --- p16 and p15 Genes --- p.13 / Chapter Chapter3: --- DNA Methylation and Cancers --- p.14 / Chapter 3.1 --- Role of DNA Methylation --- p.15 / Chapter 3.2 --- CpG Islands --- p.15 / Chapter 3.3 --- Abnormalities of DNA Methylation in Neoplasia --- p.16 / Chapter 3.3.1 --- DNA Hypomethylation in Cancer --- p.16 / Chapter 3.3.2 --- DNA Methyltransferase Activity in Cancer --- p.17 / Chapter 3.4 --- Regional DNA Hypermethylation in Cancer --- p.17 / Chapter 3.4.1 --- p16 and p15 Genes in Solid Tumors --- p.18 / Chapter 3.4.2 --- The p16 and p15 Genes in Leukemia and other Hematopoietic Malignancies --- p.19 / Chapter 3.4.3 --- Retinoblastoma Gene --- p.20 / Chapter 3.5 --- Mechanism Underlying the DNA Methylation Changes --- p.21 / Chapter Chapter4: --- Background of Study --- p.23 / Chapter 4.1 --- Background of Study --- p.24 / Chapter 4.2 --- Project Objectives --- p.27 / Chapter Chapter5: --- Materials and Methods --- p.29 / Chapter 5.1 --- Patients Samples --- p.30 / Chapter 5.2 --- Normal Controls --- p.30 / Chapter 5.3 --- Storage of the Samples --- p.32 / Chapter 5.4 --- Materials --- p.32 / Chapter 5.4.1 --- Chemicals --- p.32 / Chapter 5.4.2 --- Primers --- p.33 / Chapter 5.4.3 --- Enzymes --- p.35 / Chapter 5.5 --- Methods --- p.35 / Chapter 5.5.1 --- Cloning of p16 and p15 Exon 1 Probes for Southern Analysis --- p.35 / Chapter 5.5.1.1 --- PCR Amplification of p16 and p15 exon1 Probes from Normal Blood DNA --- p.35 / Chapter 5.5.1.2 --- Recovery and Purification of p16 and p15 Exon 1 DNA Fragment --- p.36 / Chapter 5.5.1.3 --- Ligation --- p.37 / Chapter 5.5.1.4 --- Transformation --- p.37 / Chapter 5.5.1.5 --- Plating --- p.38 / Chapter 5.5.1.6 --- Screening of Recombinant Plasmid --- p.38 / Chapter 5.5.1.7 --- Confirmation of Cloned DNA by Sequencing --- p.42 / Chapter 5.5.2 --- DNA Extraction and Purification --- p.45 / Chapter 5.5.2.1 --- DNA Extraction from Bone Marrow Aspirate and Peripheral Blood --- p.45 / Chapter 5.5.2.2 --- Isolation of Plasmid DNA from Transformant Cutures --- p.46 / Chapter 5.5.2.3 --- Qualification and Quantification of DNA --- p.49 / Chapter 5.5.3 --- Detection of Hypermethylation by Southern Analysis --- p.50 / Chapter 5.5.3.1 --- Restriction Enzyme Digestion --- p.50 / Chapter 5.5.3.2 --- Agarose Gel Electrophoresis --- p.51 / Chapter 5.5.3.3 --- Southern Transfer --- p.51 / Chapter 5.5.3.4 --- Membrane Fixation --- p.51 / Chapter 5.5.3.5 --- Recovery and Purification of p16 and p15 Exon 1 Probes from Plasmid --- p.52 / Chapter 5.5.3.6 --- Probe Labeling --- p.54 / Chapter 5.5.3.7 --- Purification of Radioactive labeled DNA --- p.54 / Chapter 5.5.3.8 --- Southern Hybridization --- p.55 / Chapter 5.5.3.9 --- Post Hybridization --- p.55 / Chapter 5.5.3.10 --- Autoradiography --- p.56 / Chapter 5.5.4 --- Polymerase Chain Reaction-Single Strand Conformational Polymorphism Analysis (PCR-SSCP) --- p.56 / Chapter 5.5.4.1 --- 5'- end Radioactive Labeling of Primer --- p.56 / Chapter 5.5.4.2 --- Amplification of Target Sequence by PCR --- p.57 / Chapter 5.5.4.3 --- Non-denaturing Polyacrylamide Gel Electrophresis --- p.57 / Chapter 5.5.4.4 --- Direct DNA Sequence of PCR Products --- p.58 / Chapter 5.5.5 --- Prevention of Overall Contamination in PCR --- p.60 / Chapter 5.5.6 --- "Sensitivity, Specificity Controls" --- p.62 / Chapter Chapter6: --- Results --- p.64 / Chapter 6.1 --- Patient Characteristics --- p.65 / Chapter 6.1.1 --- General Patient Characteristics --- p.65 / Chapter 6.1.2 --- Clinical and Laboratory Features --- p.65 / Chapter 6.2 --- Southern Blot Analysis of p16/p15 and Rb --- p.79 / Chapter 6.2.1 --- Absence of Deletions or hypermethylationin Normal Controls --- p.79 / Chapter 6.2.2 --- Absence of Homozygous Deletions or Mutationsin p16/15 and Rb among all MM Patients --- p.79 / Chapter 6.2.3 --- Hypermethylation of p16 --- p.89 / Chapter 6.2.4 --- Hypermethylation of p15 --- p.92 / Chapter 6.3 --- Hypermethylation of p16/p15 and Clinico-pathologic Correlation --- p.94 / Chapter Chapter7: --- Discussion --- p.97 / Chapter 7.1 --- "Absence of Homozygous Deletions, Gene Rearrangements and Mutations in p16/p15 and Rb" --- p.98 / Chapter 7.2 --- Hypermethylation of p16/p15-An Alternative Way for Gene Inactivation --- p.100 / Chapter 7.2.1 --- Methylation of p15 Gene --- p.101 / Chapter 7.2.2 --- Methylation of 5'-CpG Island of p16/p15 and Lack of Gene Expression --- p.102 / Chapter 7.2.3 --- Comparison of Methylation Status of Primary Samples and Cell Lines in MM --- p.103 / Chapter 7.2.4 --- Progressive Gene Inactivation by Random Methylation Errors --- p.104 / Chapter 7.2.5 --- The Lack of Correlation of Tumor Contents Revealed by the Southern Analysis and Morphologic Assessment --- p.105 / Chapter 7.3 --- Knudson's Two-hit Model of Tumorigenesis --- p.106 / Chapter 7.4 --- Inverse Relationship of p16 and Rb --- p.107 / Chapter 7.5 --- Implications of Our Findings --- p.109 / Chapter 7.6 --- Future Studies --- p.109 / References --- p.111

Cheung Kin Fai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 87-105). / Abstracts in English and Chinese. / Abstract (English version) --- p.i / Abstract (Chinese version) --- p.iii / Acknowledgments --- p.vi / Table of Contents --- p.v / List of Tables --- p.viii / List of Figures --- p.iv / List of Abbreviations --- p.xi / Chapter CHAPTER 1 --- GENERAL INTRODUCTION --- p.1 / Chapter CHAPTER 2 --- LITERATURE REVIEW --- p.3 / Chapter 2.1 --- Multiple myeloma --- p.3 / Chapter 2.2 --- Epidemiology of MM --- p.3 / Chapter 2.3 --- Risk factors --- p.4 / Chapter 2.4 --- Pathophysiology of MM --- p.5 / Chapter 2.5 --- Clinical presentations and diagnosis --- p.6 / Chapter 2.5.1 --- Diagnosis --- p.6 / Chapter 2.5.1.1 --- Laboratory testing of blood and urine --- p.6 / Chapter 2.5.1.2 --- Radiographic evaluations --- p.1 / Chapter 2.5.1.3 --- Bone marrow biopsy --- p.7 / Chapter 2.6 --- Staging and classification --- p.9 / Chapter 2.6.1 --- Staging --- p.9 / Chapter 2.6.2 --- Classification --- p.11 / Chapter 2.6.2.1 --- Monoclonal gammopathy of undetermined significance --- p.11 / Chapter 2.6.2.2 --- Asymptomatic MM --- p.12 / Chapter 2.6.2.3 --- Smouldering MM --- p.12 / Chapter 2.6.2.4 --- Indolent MM --- p.12 / Chapter 2.6.2.5 --- Symptomatic MM --- p.12 / Chapter 2.7 --- Treatment --- p.14 / Chapter 2.8 --- Epigenetics: DNA methylation --- p.15 / Chapter 2.9 --- Fundamental aspects of DNA methylation --- p.16 / Chapter 2.9.1 --- CpG islands --- p.16 / Chapter 2.9.2 --- Roles of DNA methylation --- p.16 / Chapter 2.9.3 --- Proposed mechanisms of transcriptional repression mediated by methylation --- p.18 / Chapter 2.10 --- Possible mechanisms to initiate aberrant DNA methylation --- p.21 / Chapter 2.11 --- DNA methylation in tumorigenesis --- p.22 / Chapter 2.11.1 --- Oncogenic point C → T mutation --- p.22 / Chapter 2.11.2 --- Global DNA hypomethylation --- p.23 / Chapter 2.11.3 --- Regional DNA hypermethylation --- p.23 / Chapter 2.12 --- Aberrant DNA methylation in MM --- p.25 / Chapter 2.12.1 --- Self-sufficiency in growth signals --- p.25 / Chapter 2.12.2 --- Evading apoptosis --- p.26 / Chapter 2.12.3 --- Insensitivity to antigrowth signals --- p.26 / Chapter 2.12.4 --- Tissue invasion and metastasis --- p.27 / Chapter 2.12.5 --- Infinite replicative potential --- p.28 / Chapter 2.12.6 --- Genome instability --- p.30 / Chapter 2.13 --- Methodologies of DNA methylation analysis --- p.32 / Chapter 2.13.1 --- Genome wide screening method: MS.AP-PCR --- p.32 / Chapter 2.13.2 --- Combined bisulfite restriction analysis --- p.34 / Chapter 2.13.3 --- Cloned bisulfite genomic sequencing --- p.36 / Chapter 2.13.4 --- Treatment with demethylating agent --- p.36 / Chapter CHAPTER 3 --- MATERIALS AND METHODS --- p.38 / Chapter 3.1 --- MM specimens --- p.38 / Chapter 3.1.1 --- MM samples --- p.38 / Chapter 3.1.2 --- MM cell lines --- p.38 / Chapter 3.2 --- Magnetic cell sorting of CD138-positive plasma cells --- p.39 / Chapter 3.3 --- Isolation of nuclear pellet from PB --- p.41 / Chapter 3.4 --- "DNA extraction from MM cell lines, MM plasma cells and PB" --- p.41 / Chapter 3.5 --- MS.AP-PCR --- p.42 / Chapter 3.5.1 --- Restriction enzyme digestion of genomic DNA --- p.42 / Chapter 3.5.2 --- Arbitrarily primed polymerase chain reaction --- p.42 / Chapter 3.5.3 --- Isolation of differentially methylated DNA fragments --- p.43 / Chapter 3.6 --- Cloning of differentially methylated DNA fragments --- p.46 / Chapter 3.6.1 --- TA cloning --- p.46 / Chapter 3.6.2 --- Heat shock transformation --- p.46 / Chapter 3.6.3 --- Screening of positive clones by PCR --- p.46 / Chapter 3.6.4 --- Alkaline lysis for plasmid DNA preparation --- p.47 / Chapter 3.7 --- MS.AP-PCR sequence analysis --- p.47 / Chapter 3.7.1 --- Nucleotide sequencing --- p.47 / Chapter 3.7.2 --- CpG islands analysis of differentially methylated sequences --- p.48 / Chapter 3.8 --- DNA methylation analysis --- p.48 / Chapter 3.8.1 --- Sodium bisulfite modification --- p.48 / Chapter 3.8.2 --- Combined bisulfite restriction analysis --- p.49 / Chapter 3.8.3 --- Cloned bisulfite genomic sequencing --- p.49 / Chapter 3.9 --- Gene expression analysis --- p.50 / Chapter 3.9.1 --- RNA extraction --- p.50 / Chapter 3.9.2 --- Reverse transcription PCR --- p.50 / Chapter 3.9.3 --- 5'-aza-2'-deoxycytidine treatment --- p.51 / Chapter CHAPTER 4 --- RESULTS --- p.53 / Chapter 4.1 --- Generation of DNA methylation patterns by MS.AP-PCR --- p.53 / Chapter 4.1.1. --- Global methylation content in MM samples and normal PB lymphocytes --- p.56 / Chapter 4.1.2. --- Differential methylation in MM --- p.56 / Chapter 4.2 --- UCSC BLAT analysis of differentially methylated DNA fragments --- p.60 / Chapter 4.3 --- Identification of two candidate genes with downregulated expression --- p.60 / Chapter 4.4 --- Zinc fingers and homeoboxes 2 (ZHX2) --- p.62 / Chapter 4.4.1 --- ZHX2 CpG islands BLAT search analysis --- p.62 / Chapter 4.4.2 --- Hypermethylation of ZHX2 in MM cell lines --- p.63 / Chapter 4.4.3 --- Downregulated expression of ZHX2 in methylated MM cell lines --- p.66 / Chapter 4.4.4 --- Restoration of ZHX2 expression by 5-Aza-dC treatment --- p.67 / Chapter 4.4.5 --- Unmethylation of ZHX2 in primary MM tumors --- p.68 / Chapter 4.5 --- Ring finger protein 180 (RNF180) --- p.69 / Chapter 4.5.1 --- RNF180 CpG islands BLAT search analysis --- p.69 / Chapter 4.5.2 --- Hypermethylation of RNF180 in MM cell lines --- p.70 / Chapter 4.5.3 --- Downregulated expression of RNF180 in methylated MM cell lines --- p.73 / Chapter 4.5.4 --- Restoration of RNF180 expression by 5-Aza-dC treatment --- p.74 / Chapter 4.5.5 --- Methylation of RNF180 in primary MM tumors --- p.75 / Chapter CHAPTER 5 --- DISCUSSION --- p.76 / Chapter 5.1 --- Importance of methylation in MM --- p.76 / Chapter 5.2 --- Genome-wide screening approach by MS.AP-PCR --- p.76 / Chapter 5.3 --- Sample selection in MS.AP-PCR --- p.78 / Chapter 5.4 --- Methylation patterns in MM --- p.79 / Chapter 5.5 --- Candidate genes selection strategies --- p.81 / Chapter 5.6 --- Zinc fingers and homeoboxes 2 --- p.81 / Chapter 5.7 --- Ring finger protein 180 --- p.83 / Chapter 5.8 --- Limitations --- p.84 / Chapter CHAPTER 6 --- CONCLUSION --- p.86 / REFERENCES --- p.87

Cheung, Hing Yau Coty. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2013. / Includes bibliographical references (leaves 111-132). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstracts also in Chinese.

Cheng Suk Hang. / "February 2005." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (p. 142-160) / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Ng Heung-ling, Margaret. / "April 2003." / Thesis (M.D.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (p. 216-237). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web.

Wong, Ling Yee. / Thesis submitted in: August 2007. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 110-127). / Abstracts in English and Chinese. / Thesis Abstract --- p.i / 論文摘要 --- p.iv / Acknowledgements --- p.vi / Abbreviations --- p.vii / Thesis Content --- p.xii / List of Figures --- p.xv / List of Tables --- p.xvii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter Chapter 2 --- Literature Review --- p.3 / Chapter 2.1. --- Multiple Myeloma (MM) --- p.3 / Chapter 2.1.1 --- Epidemiology --- p.4 / Chapter 2.1.2 --- Cause and Risk Factors --- p.5 / Chapter 2.1.3 --- Pathophysiology --- p.5 / Chapter 2.1.4 --- Diagnosis and Clinical Presentation --- p.6 / Chapter 2.1.5 --- Classification of Plasma Cell Disorders --- p.6 / Chapter 2.1.5.1 --- Monoclonal Gammopathy of Undetermined Significance (MGUS) --- p.6 / Chapter 2.1.5.2 --- Asymptomatic (Smouldering) MM --- p.7 / Chapter 2.1.5.3 --- Indolent MM --- p.7 / Chapter 2.1.5.4 --- Symptomatic MM --- p.8 / Chapter 2.1.6 --- Staging --- p.9 / Chapter 2.1.7 --- Treatment --- p.11 / Chapter 2.1.8 --- Molecular Abnormality --- p.12 / Chapter 2.2 --- DNA Microarray Analysis in MM --- p.13 / Chapter 2.2.1 --- MM Pathogenesis --- p.15 / Chapter 2.2.2 --- Molecular Classification of MM --- p.18 / Chapter 2.2.3 --- Anti-MM Drug Studies --- p.22 / Chapter 2.3 --- Cancer Treatment Response Prediction --- p.24 / Chapter 2.3.1 --- MP Treatment --- p.24 / Chapter 2.3.1.1 --- Melphalan --- p.25 / Chapter 2.3.1.2 --- Prednisone --- p.27 / Chapter 2.3.1.3 --- MP Treatment Response Prediction in MM --- p.29 / Chapter 2.3.2 --- Cancer Prognosis using DNA Microarray --- p.31 / Chapter Chapter 3 --- Materials and Methods --- p.36 / Chapter 3.1. --- Patient Specimens for Gene Expression Profiling and Quantitative Real-time PCR --- p.36 / Chapter 3.2. --- Magnetic Cell Sorting of CD138-positive Plasma Cells --- p.37 / Chapter 3.2.1 --- Density Gradient Centrifugation --- p.37 / Chapter 3.2.2 --- Positive Selection of CD138-positive Cells --- p.37 / Chapter 3.3 --- Generation of Gene Expression Profiles --- p.39 / Chapter 3.3.1 --- RNA Extraction --- p.39 / Chapter 3.3.2 --- RNA Assessment --- p.40 / Chapter 3.3.3 --- Synthesis and Purification of Double-strand cDNA --- p.40 / Chapter 3.3.4 --- In vitro Transcription (IVT) and Recovery of Biotin-labeled cRNA --- p.41 / Chapter 3.3.5 --- cRNA Fragmentation and Hybridization Reaction Mixture Preparation --- p.41 / Chapter 3.3.6 --- Hybridization --- p.42 / Chapter 3.3.7 --- Post-hybridization Wash --- p.42 / Chapter 3.3.8 --- Detection with Streptavidin-dye Conjugate --- p.43 / Chapter 3.3.9 --- Bioarray Scanning and Spot Signal Quantitation --- p.43 / Chapter 3.4 --- Microarray Data Analysis --- p.45 / Chapter 3.4.1 --- Normalization and Filtering --- p.45 / Chapter 3.4.2 --- Unsupervised Clustering Analysis --- p.45 / Chapter 3.4.3 --- Supervised Class Comparison Analysis --- p.46 / Chapter 3.5 --- Microarray Verification and Candidate Gene Validation --- p.47 / Chapter 3.5.1 --- RNA Extraction --- p.47 / Chapter 3.5.2 --- Reverse Transcription PCR --- p.47 / Chapter 3.5.3 --- Quantitative Real-time PCR --- p.48 / Chapter 3.6 --- Predictive Value Calculation --- p.49 / Chapter 3.7 --- Experimental Flow --- p.49 / Chapter Chapter 4 --- Results --- p.53 / Chapter 4.1 --- Gene Expression Profiling of Chinese MM --- p.53 / Chapter 4.1.1 --- Unsupervised Clustering Analysis --- p.53 / Chapter 4.1.1.1 --- Hierarchical Clustering --- p.53 / Chapter 4.1.1.2 --- Principal Component Analysis (PCA) --- p.54 / Chapter 4.1.2 --- Identification of Statistically Differentially Expressed Genes --- p.58 / Chapter 4.1.2.1 --- Two-Sample t-statistics --- p.58 / Chapter 4.1.2.2 --- Significance Analysis of Microarrays (SAM) --- p.58 / Chapter 4.1.2.3 --- Microarray Verification --- p.66 / Chapter 4.2 --- Development of MP Treatment Response Biomarker in MM --- p.70 / Chapter 4.2.1 --- Unsupervised Clustering Analysis --- p.70 / Chapter 4.2.1.1 --- Hierarchical Clustering --- p.70 / Chapter 4.2.1.2 --- PCA --- p.70 / Chapter 4.2.2 --- Identification of Statistically Differentially Expressed Genes --- p.74 / Chapter 4.2.2.1 --- Two sample t-statistics --- p.74 / Chapter 4.2.2.2 --- SAM --- p.74 / Chapter 4.2.3 --- Verification of Candidate Gene CYB5D1 --- p.76 / Chapter Chapter 5 --- Discussion --- p.79 / Chapter 5.1 --- Global Gene Expression Profiling: DNA Microarray --- p.79 / Chapter 5.2 --- Microarray Data Normalization and Gene Filtering --- p.81 / Chapter 5.3 --- Microarray Data Analysis --- p.83 / Chapter 5.3.1 --- Unsupervised Clustering Analysis --- p.83 / Chapter 5.3.1.1 --- Hierarchical Clustering --- p.83 / Chapter 5.3.1.2 --- PCA --- p.85 / Chapter 5.3.2 --- Identification of Statistically Differentially Expressed Genes --- p.86 / Chapter 5.4 --- Verification of Candidate Genes by Quantitative Real-time PCR --- p.89 / Chapter 5.5 --- Gene Expression Profiling of Chinese MM --- p.90 / Chapter 5.5.1 --- Comparison of Gene Expression Patterns of MM and Normal Plasma Cells --- p.90 / Chapter 5.5.2 --- Differentially Expressed Genes between MM and Normal Plasma Cells..… --- p.91 / Chapter 5.5.2.1 --- Common Differentially Expressed Genes with Previous Studies --- p.94 / Chapter 5.5.2.2 --- Potential Tumor Suppressor Genes in Differentially Expressed Genes..… --- p.96 / Chapter 5.5.2.3 --- Verified Differentially Expressed Genes --- p.98 / Chapter 5.5.3 --- Future Studies --- p.101 / Chapter 5.6 --- Development of MP Treatment Response Biomarker in MM --- p.103 / Chapter 5.6.1 --- Comparison of Gene Expression Patterns of MP Good Responders (GR) and Poor Responders (PR) --- p.103 / Chapter 5.6.2 --- Differentially Expressed Gene between MP GR and PR: CYB5D1 --- p.104 / Chapter 5.6.3 --- Possible Role of CYB5D1 in MP Resistance in MM Cells --- p.104 / Chapter 5.6.4 --- Potential Clinical Application of CYB5D1 in MP Treatment Response Prediction in MM --- p.106 / Chapter 5.6.5 --- Future Studies --- p.106 / Chapter Chapter 6 --- Conclusion --- p.108 / Chapter 6.1 --- Gene Expression Profiling of Chinese MM --- p.108 / Chapter 6.2 --- Development of MP Treatment Response Biomarker in MM --- p.108 / References --- p.110 / Appendix --- p.128