Nematicides are not only expensive and unaffordable to small-scale farmers but are also harmful to the environment as they kill both the target organisms and non-target micro and macro-organisms, thereby destabilising the ecosystem. Most developed countries have or are in the process of banning use of chemicals for pest management, implying that agricultural products from developing countries using pesticides will not be marketed in the developed countries. In former studies, it was shown that plant parasitic nematodes posed serious problems in sugarcane fields as their attacks on sett roots during germination period decreased sett root weights, delayed bud germination or led to fewer buds germinating as most buds abort. Those that germinate later are then faced with competition for food, space and light from the "older" shoots and often die. The attacks on shoot roots may lead to inefficient uptake of water and nutrients by the plant thereby leading to stunted plants. Fewer and shorter sugarcane stalks due to nematode attacks result in poor yield. In this study, it has been shown that a large number of endoparasites would be needed to reduce sett root weights. Agricultural systems based on monoculture are rarely successful in the long term and because sugarcane fields have been monocultured for a very long time, they are losing their productive capacity and this is termed "Yield Decline". Instead of using nematicides, alternative methods can be used for the management of nematode communities. Research has shown on other plants that nematode communities dominated by Helicotylenchus dihystera are less pathogenic to the plants than other ectoparasitic nematodes, e.g., Xiphinema elongatum and Paratrichodorus spp. A study conducted as a pot experiment showed that sugarcane grown in soil with high H. dihystera grew taller and produced greater root and aerial biomass than one grown in X elongatum infested soil. To induce a nematode community dominated by H. dihystera in the field, two strategies were followed: (i ) abiotic factors that influence the nematode's environment were identified. Certain elements found in soil and sugarcane leaves were found to be correlated to certain species, e.g., H. dihystera was negatively correlated to soil sulphur, medium and coarse sand while X elongatum was positively correlated to these soil types and soil elements. Sugarcane leaves with high levels of Ca, Zn, Cu and Fe were found in areas with high percentages of H. dihystera while the reverse was true for X elongatum. (ii) organic amendments were used to improve the sugarcane growth, modify the environment and decrease competition among species within a community. Application of organic matter to the soil improves soil properties such as water infiltration, water holding capacity, erodibility and nutrient cycling, increases suppressiveness of soils to plant parasitic nematodes and stimulates other anti-nematode micro-organisms, e.g., nematode-trapping fungi. Organic amendments were therefore used in this study not only as screens to protect sugarcane roots from nematode attacks but also to manipulate nematode communities for the less pathogenic species, H. dihystera. In a field study where organic amendments were used, plots treated with filter cake, thume + filter cake, trash + filter cake, filter cake + furfural and Temik (aldicarb) had high percentages of H. dihystera while control plots had high percentages of X elongatum. However, the change in relative proportion of H. dihystera by certain treatments was not followed by an average increase in yield, probability because of the overall variability. The yield results, however, showed that for all treatments, including control, the highest yields corresponded to plots with higher H. dihystera proportions, conflicting the initial hypothesis. As a result, if an organic amendment that can substantially increase the relative proportions of H. dihystera can be found, a substantial increase in yield can be expected. Although the organic amendments did not successfully manipulate the nematode communities for the less pathogenic species, H. dihystera, plots with higher yield were those that had high H. dihystera percentages in their nematode communities. / Thesis (Ph.D.)-University of Natal, Durban, 2004.

In the summer of 1997, 217 fishes of different species (Raja erinacea, R. radiata, R. ocellata, Malacoraja senta, and Squalus acanthias) were collected from the West Isles of the Bay of Fundy, New Brunswick. / Parasites belonging to 13 species were found: 10 Cestoda; 2 Nematoda; and 1 Acanthocephala. Of the 10 cestodes, five are described as new species. The remaining five re-described in detail and compared to similar worms reported in the literature. None of the eight tetraphyllidean cestodes was found in more than one host species, thereby supporting current dogma that the Tetraphyllidea are species specific with respect to their definitive hosts. / There is a close relationship between the topography of the spiral-valve mucosa of the preferred attachment-site and that of the inner-bothridial tegument. This provides further evidence supporting the strict morphological specificity proposed by Williams (1960).

Includes bibliographical references. (p. 131-140) / 140 leaves : ill. (some col.) ; 26 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Zoology, 1968

Attempts were made to grow Fergusobia nematodes in a dual aseptic culture with Eucalyptus camaldulensis. Callus tissue was grown from E. camaldulensis stem pieces in aseptic conditions. Calli were prone to deterioration after 14 days unless transferred to fresh growth medium. Lower levels of solutes (25% Murashige and Skoog salts, 25% plant vitamins and 0.5% sucrose) were more successful than published concentrations. Fergusobia J2 nematodes were surface sterilised with either Hibitane or washed with water to prepare them for inoculation of callus (Hay, 1994). Fergusobia subsequently recovered from plates of callus were all dead, which raised questions of how the nematodes are suited to Murashige and Skoog salt solutions. The survival of Fergusobia in aqueous solutions was then observed. It has been assumed that Fergusobia live about 2 days after dissection. Amphimictic nematodes from E. camaldulensis axial bud galls were used for a survival study. Nematodes lived for as long as 12 days in fresh tap water and 11 days in 1% M.S. solution. They were more active in tap water than in 1% M.S. solution. Nematodes in a dish together with dissected gall material died within 2 days. Nematodes in a separate dish with fresh tap water and clean gall fibres were observed to gather around the fibres. Observations of Fergusobia could be made within fresh solutions providing deteriorating gall tissues were removed from the dish. Gall production was attempted on E. camaldulensis grown in the glasshouse. These tree saplings were pruned to encourage new growth and periodically treated for infestations of scale insects leading to growth of sooty mould. Two forms of cage construction were used: (1) 1 m ³ screened cages and (2) acetate sleeves as used by Goolsby et al., 2000. Within the 1 m ³ cages containing flies, the growing points on saplings were blackened, possibly due to over-exploitation by ovipositing flies. One growing point caged in an acetate sleeve showed oviposition scars but did not produce a mature gall. Production of galls in the glasshouse was hindered by a lack in coincidence of flies emerging from mature galls and the flush of new growth following pruning. The production of galls within the glasshouse was not achieved. The phenology of E. camaldulensis, a host for the Fergusonina/Fergusobia mutualism, and gall ecology were observed in a two-year, non-destructive, field study in the Urrbrae Wetlands, Adelaide, South Australia. Tree growth and gall development was observed in the lower regions (0 – 2 m) of young trees. Three bud forms, terminal leaf bud, axial leaf bud and flower bud galls were monitored on the trees. The densities of galls were highly seasonal. Greatest density of growing points, axial leaf bud and flower bud galls occurred mostly during mid-winter to spring, whereas that of terminal leaf bud galls occurred during mid-spring to summer. Galling of flower buds did not appear to influence flowering and more flower buds and flowers occurred in the second year of the study as the trees matured. Trees mostly had medium (30-70%) levels of leaf damage, but there were seasonal trends in damage levels. Low scores for leaf damage were associated with increases in flower bud and flower production. Leaf damage, including sooty mould, appeared to increase during the cooler winter months. There were no significant seasonal relationships between levels of leaf damage and either growing point density or the occurrence of galls. When trees were compared with each other, those with lower leaf damage were more likely to have more growing points. The appearance of the canopy and the likelihood of a tree to have galls varied greatly between the trees. One tree was particularly susceptible to leaf attack, rarely had new leaves and produced no mature galls during the study. The colouring of leaves varied between trees, which indicates possible genetic variations causing some trees to be more likely hosts for Fergusonina/Fergusobia. Both new vegetative growth and terminal leaf bud galls were concentrated on the northern and eastern quadrants. Axial leaf bud and flower bud galls occurred more on the western or southern quadrants where they were possibly more protected from sun exposure on the northern or eastern quadrants. Axial galls on the northern side of one tree became reddened while those in the southern and western quadrants remained green. Reddening of axial galls may increase their likelihood of parasitism and predation by birds. Each of the three gall forms occurred within certain positions in the canopy. The tree host resource is partitioned effectively, with the three gall forms occurring on three different host structures. Additionally, the two vegetative forms terminal leaf bud and axial leaf bud galls occur on different shoot regions and in different seasons. The numbers of the galls is probably also affected by biotic and climatic influences. Parasitism, plant canopy shading, nutrient levels and host genetics are possible influences. Assuming an interval of 4 weeks between oviposition and first observation within the current study, terminal leaf bud galls had an average longevity of 11 weeks and axial leaf bud galls an average longevity of 14 weeks. Flower bud galls had longevities of 14 to 27 weeks from oviposition to senescence, assuming an interval of 6 weeks between oviposition and first observation within the current study. Flowers and flower buds occur irregularly within the eucalypts so it would be advantageous for flies and nematodes developing within flower bud galls to have extended or variable longevities to allow fly emergences to coincide with new flower buds. Not all of the galls recorded matured to produce adult flies. Nearly half of the terminal leaf bud galls initiated were aborted, recorded as absent, parasitised or eaten (45% of initiated galls). Of the three gall forms, they were the most prone to obvious parasitism and as many as 12 hymenopteran species have been reared from terminal leaf bud galls on E. camaldulensis (Taylor et al., 1996). These galls obviously provide a resource for many species within multiple trophic levels. Fourteen percent of axial leaf bud galls were absent or eaten and birds were seen breaking off and feeding on the galls. More than half (55%) of the initiated flower bud galls disappeared during the period of observation, possibly due to the foraging of birds. Destructive sampling and rearing out of parasitoids from both axial leaf bud and flower bud gall forms is needed to establish what species exist within them. Terminal leaf bud galls ranged from 7.5 to 30.1 mm in diameter and 10.0 to 43.6 mm in length. Flower bud gall size varied, with the largest being 15.0 mm by 22.3 mm. Axial leaf bud galls, ranged from 2.6 to 13.0 mm in diameter and length ranged from 2.3 to 10.5 mm. The larger axial leaf bud galls were nodular and appeared to have multiple locules. Destructive sampling and rearing out of flies is needed to establish the relationship between size and numbers of flies emerging. Terminal leaf bud galls increased in size, including many locules and exit holes per gall. Axial leaf bud galls were much smaller than the terminal leaf bud galls and 99% had only one to three exit holes. The rounded shape and presence of few locules within the axial leaf bud galls indicate that this form is limited to a shape and size producing few flies. The observation of greater size of terminal leaf bud galls suggests that these galls may have multiple foundresses. Twelve of the 13 flower bud galls with exit holes had either one or two holes. In flower bud galls on E. camaldulensis. the operculum remains sealed and the characteristic Fergusonina “window” appears at the side of the flower bud before fly emergence through a single hole. Destructive sampling is also necessary to determine parasitism of each of the gall forms. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1331016 / Thesis (M.Sc.) -- University of Adelaide, School of Agriculture, Food and Wine, 2008

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2007. / Härtill 5 uppsatser.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniversitet, 2006. / Härtill 4 uppsatser.

Diss. (sammanfattning) Alnarp : Sveriges lantbruksuniversitet, 2008. / Härtill 4 uppsatser.

Submitted by Alberto Vieira (martins_vieira@ibest.com.br) on 2018-01-12T16:09:59Z No. of bitstreams: 1 552188.pdf: 13006169 bytes, checksum: 8bf2dc666edb41bf9ed601e7ecf7af4c (MD5) / Made available in DSpace on 2018-01-12T16:10:00Z (GMT). No. of bitstreams: 1 552188.pdf: 13006169 bytes, checksum: 8bf2dc666edb41bf9ed601e7ecf7af4c (MD5) Previous issue date: 2002 / Determina a composição genérica, a estrutura trófica e a estrutura da comunidade de nematódeos marinhos da planície de maré de Coroa Grande, Baía de Sepetiba, Rio de Janeiro, Brasil (22º 55' 00"S e 43º05' 30"W). Amostras mensais foram coletadas durante a baixa-mar, usando-se um amostrador de 1cm², no período de maio/1998 a abril/1999. As amostras foram obtidas em perfis com estratos que representaram os diferentes níveis da zona entre-marés. Também. coletaram-se amostras para determinar a composição granulométrica, o teor de matéria orgânica e a concentração de clorofila a. A análise canônica de correspondência foi utilizada para avaliar a estrutura da comunidade. Duas diferentes classificações foram utilizadas para determinar a estrutura trófica da comunidade. Um total de 31 gêneros foi encontrado: Anoplostoma, Bathylainnus, Comesoma, Daptonema, Enoplolainnus, Eurystomina, Halichoanolaimus, Haliplectus, Metachromadora, Oxystomina, Oncholainnus, Paracanthonchus, Paraodontophora, Sabatieria, Steineria, Sphaerolaimus, Spirinia, Terschellingia, Theristus, Viscosia e Trissonchulus. Os gêneros Chromadorella, Chromaspirinia, Dorylaimopsis, Euchromadora, Gomphionema, Gammanema, Linhomoeus e Paramonohystera apresentaram o seu primeiro registro de ocorrência para a costa brasileira. A composição faunística não foi diferente de outros hábitats similares. A análise canônica de correspondência mostrou uma estrutura espaço-temporal da comunidade de nematódeos, com as amostras dos níveis inferiores da planície de maré (próximos a zona infralitoral) formando um grupo separado das amostras dos níveis superiores. Os padrões de diversidade mostram-se influenciados pelas características sedimentológicas do ambiente, especialmente, a quantidade de finos. Os valores de diversidade encontrados para a área de estudo são semelhantes aos de outros estudos realizados em áreas tropicais, no entanto, são mais baixos quando comparados aos valores obtidos em áreas temperadas. Os padrões gerais da estrutura trófica da comunidade estão associados às variações na abundância dos três principais gêneros: Comesoma, Daptonema e Oncholaimus. Em termos espaciais, os comedores de depósito stricto sensu são dominantes nos níveis inferiores da planície de maré, enquanto os predadores facultativos predominam nos níveis superiores. / Determine the generic composition, trophic structure and community structure of marine nematodes on the Coroa Grande tidal flat, Sepetiba bay, Rio de Janeiro. Brazil (22º55'00"S e 43º05' 30''W). Monthly samples were taken during Iow tides, using a corer with 1cm², from may-98 to april-99. Samples were obtained within transect strata which represent the different levels of the intertidal zone. Samples for granulometric composition, organic matter anel chlorophyll analysis were also collected. Canonical correspondence analysis (CCA) was used to evaluate the community structure. Two different classifications were used in order to determine the trophic structure. The structure of nematode community showed a spatial-temporal. with samples of the lower tidal flat level (near to the subtidal zone) forming a separate group from samples of the upper levels. A total of 31 genera were found: Anoplostoma, Bathylainnus, Comesoma, Daptonema, Enoplolainnus, Eurystomina, Halichoanolaimus, Haliplectus, Metachromadora, Oxystomina, Oncholainnus, Paracanthonchus, Paraodontophora, Sabatieria, Steineria, Sphaerolaimus, Spirinia, Terschellingia, Theristus, Viscosia and Trissonchulus. The genera Chromadorella, Chromaspirinia, Dorylaimopsis, Euchromadora, Gomphionema, Gammanema, Linhomoeus and Paramonohystera were recorded for the first time in Brazilian sediments. The faunal composition was not very different from other similar habitats. Diversity patterns were influenced by the sedimentological characteristics of the environment, especially, the amount of fine sediments. Diversity values found on the studied arca were similar to those of different studies in tropical areas; nevertheless are low when compared to temperate areas. The general patterns of lhe trophic structure were associated with the change in the abundance of the three main genera: Comesoma, Daptonema e Oncholaimus. In spatial terms, deposit feeders stricto sensu predominated in the lower level of the tidal flat, while facultative predators dominated the upper levels.

Made available in DSpace on 2014-06-11T19:28:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-02-26Bitstream added on 2014-06-13T19:37:24Z : No. of bitstreams: 1 gabia_aa_me_botfca.pdf: 416606 bytes, checksum: 894930ec055a3224888bc51077855bce (MD5) / Tylenchulus semipenetrans é o principal nematoide em citros, causando perdas que variam de 10 a 30%. Está amplamente disseminado nos pomares citrícolas do Brasil. Para determinar o nível populacional desse nematoide, que varia durante o ano, diferentes variáveis são utilizadas. Sendo assim o objetivo deste trabalho foi avaliar a flutuação populacional do nematoide T. semipenetrans em pomar de laranja ‘Pera Rio’ enxertada em limoeiro ‘Cravo’, correlacionar a população do nematoide no final de cada estação com a produção e correlacionar o número de fêmeas na raiz com o número de juvenis encontrados nas amostras de solo e raiz. As amostragens foram realizadas no final de cada estação climática, durante a safra 2011/2012, numa profundidade de 0-30 cm, na projeção da copa de 10 plantas sem sintomas, 10 com sintomas e as adjacentes das sintomáticas, totalizando 60 plantas. Os nematoides presentes no solo foram extraídos pela metodologia de flutuação em centrifuga e das raízes pela técnica do liquidificador e centrifugação. Os frutos de cada planta foram colhidos separadamente e pesados. Para contagem das fêmeas, 1g de raiz de cada amostra foi submetida à coloração para facilitar a contagem. O número de nematoides no solo e na raiz foram plotados em gráficos e correlacionados com dados de temperatura e precipitação pluviométrica pela análise de Pearson; os dados de peso de fruto por planta e número de nematoides em solo... / Tylenchulus semipenetrans is the main nematodes in citrus trees, causing losses ranging from 10 to 30%. It is widespread in the citrus orchards of Brazil. To determine the level of nematode population which varies over the year, different variable are used. Therefore the objective of this study was to evaluate the fluctuation of nematode T. semipenetrans on acreage with orange ‘Pera Rio’ grafted on ‘Rangpur’, correlate the nematode population at the end of each season with the production and correlate the number of females in the root with the number of juveniles found in soil samples and root . Samples were taken at the end of each season climate, during the 2011/2012 season, at a depth of 0-30 cm, the crown projection of 10 plants without symptoms, 10 with symptoms and symptomatic adjacent plant, totaling 60 plants. The nematodes in the soil were extracted by centrifugal flotation method and the technique of the roots of the blender and centrifugation. The fruits of each plant were harvested and weighed separately. To count the number of females in the roots, 1g of each root sample was colored and subsequently dried. The number of nematodes in soil and roots were plotted on graphs and correlated with data on temperature and rainfall by Pearson analysis, the data for fruit weight per plant and the number of nematodes in soil and roots, on each season, were transformed and analyzed by Spearman correlation, considering both the significance level of 5%. The data on soil nematode, root nematode, sum of soil and root nematode and females per gram of root were analyzed by Pearson correlation analysis and subsequent to the correlation matrix. In the region of Botucatu, central western state of São Paulo, the largest population level of the citrus nematode occurred in late winter and lowest in late spring, and the nematodes... (Complete abstract click electronic access below)

Orientador: Pedro Luiz Martins Soares / Coorientador: Antônio de Goes / Banca: Rita de Cássia Panizzi / Banca: Daniel Junior de Andrade / Banca: Maria Amélia dos Santos / Banca: Bruno Flávio Figueiredo Barbosa / Resumo: A heveicultura assume papel de destaque no estado de São Paulo, que responde por mais da metade da produção Brasileira de borracha natural. Os nematoides são importantes patógenos que vêm causando perdas significativas à produção de diversas plantas cultivadas. Na literatura, encontram-se poucos trabalhos acerca dos nematoides na cultura da seringueira, principalmente quando se trata de Pratylenchus brachyurus, um nematoide de grande importância atual no Brasil. Neste sentido os objetivos deste trabalho foram: a) realizar um levantamento dos nematoides em viveiros de produção de mudas de seringueira do estado de São Paulo, b) avaliar a resistência e tolerância de plantas de seringueira utilizadas como porta enxertos à Pratylenchus brachyurus e Meloidogyne exigua, c) estudar a interação entre M. exigua ou P. brachyurus, e Lasiodiplodia theobromae em mudas de seringueira em duas condições hídricas. Os resultados do levantamento demonstram que os nematoides de maior frequência e densidade foram Meloidogyne e Pratylenchus. As principais espécies identificadas foram M. incognita, M. exigua, P. brachyurus e Rotylenchulus reniformis. Destes, P. brachyrus foi a espécie mais frequente nas amostras das raízes das mudas de seringueira e deve ser considerado praga chave da cultura. Este trabalho também foi primeiro relato de M. exigua no Estado de São Paulo causando danos em mudas de seringueira. O segundo experimento realizado em casa de vegetação, demonstrou que M. exigua causou reduções mais pronunciadas na altura e no diâmetro de caule dos porta-enxertos de seringueira 'GT 1', 'PB 235', 'PB 217', 'RRIM 501', 'PR 255', 'IAN 873', 'RRIM 600' e 'TJ 1', sendo todos considerados intolerantes e suscetíveis a M. exigua e P. brachyurus. E por fim, no terceiro experimento, realizado em casa de vegetação, observou-se que tanto as características biométricas, quanto os nematoides foram afetados... / Abstract: The heveiculture plays an important hole in São Paulo State, which produces more than 50% of the total Brazilian natural rubber production. The nematodes are important pathogens that have been causing significant losses to the crops. In the literature there is not many papers related to nematodes in rubber trees, especially about Pratylenchus brachyurus, the nematode most important to Brazil nowadays. In this sense the objectives of this work were: a) to do a survey of the nematodes occurrence in rubber trees nurseries in São Paulo State, b) study the resistance and tolerance of rubber trees used as rootstock to Pratylenchus brachyurus and Meloidogyne exigua, c) study the interactions between M. exigua or P. brachyurus, and Lasiodiplodia theobromae in rubber trees seedlings in two moisture conditions. As the results of the survey it was found that Meloidogyne and Pratylenchus were the most frequent and in high densities at the samples. The species identified were M. incognita, M. exigua, P. brachyurus e R. reniformis. Among them, P. brachyurus was the most frequent specie found at rubber trees roots and must be consider as a key nematode to the crop. This work was also the first report of M. exigua causing damage to the rubber trees seedlings in São Paulo State. The second experiment carried out at green house conditions, have demonstrate that M. exigua caused more pronounced damage to the rubber tree rootstocks 'GT 1', 'PB 235', 'PB 217', 'RRIM 501', 'PR 255', 'IAN 873', 'RRIM 600' e 'TJ 1', and all of them were intolerant and susceptible to M. exigua e P. brachyurus. Finally, at the third experiment also carried out in green house conditions, was observed that the biometrics characteristics and the nematodes were affected by the reduction in 50% (CC) of pot capacity. There was an interaction between L. theobromae and P. brachyurus in the increase of plant diameter. There was not an interaction among ... / Doutor