Hipertensão do avental branco significa uma elevação persistente da pressão arterial no consultório médico ou clínica, com pressão normal em quaisquer outras circunstâncias. Existem diversos questionamentos a respeito da origem, significado clínico, prognóstico e tratamento desta manifestação. Em relação à etiologia, nossa hipótese é que uma alteração endotelial, resultando em deficiência na produção ou utilização de óxido nítrico endógeno, constitua um fator primário para a ocorrência da hipertensão do avental branco. Este estudo, desenvolvido entre moradores do município de Dumont - São Paulo, Brasil, teve como objetivos caracterizar os participantes em relação a fatores demográficos, alterações fisiológicas e metabólicas para posteriormente identificar e comparar os níveis plasmáticos de nitrato - produto da degradação do óxido nítrico ? entre os sujeitos da pesquisa. De uma amostra de 441 voluntários, selecionamos 109 indivíduos, que foram divididos em três grupos: normotensão (no=58), hipertensão essencial (no=33) e hipertensão do avental branco (no=18), após medidas de pressão arterial com aparelho oscilométrico e exame de Monitorização Ambulatorial da Pressão Arterial. Realizamos entrevista, mensuração de dados e coleta de exames laboratoriais para comparação das variáveis encontradas entre os grupos. Para o tratamento estatístico, foram utilizados os testes ANOVA e Tukey. Os resultados foram expressos como médias ± erros padrões das médias. As diferenças foram consideradas estatisticamente significativas para p<0,05. A prevalência de hipertensão do avental branco foi de 34,1%, com predominância do sexo feminino (83,3%), média de idade de 45,28 anos, sendo a maioria natural do Estado de São Paulo (66,7%), de cor branca (88,9%), alfabetizada (33,3%), casada (72,2%), com histórico familiar para doenças cardiovasculares (72,2%). A análise da quantificação de nitrato plasmático apontou diferença significativa entre os grupos hipertensão do avental branco e normotensão em comparação aos hipertensos, com elevação dos níveis de nitrato sérico em portadores de hipertensão essencial. Também encontramos diferença estatisticamente significativa para índice de massa corporal, relação cintura/quadril, glicemia e creatinina plasmáticas, na comparação entre hipertensos do avental branco e normotensos. As distinções observadas entre os grupos e a presença de variações clínicas, demográficas e bioquímicas possibilitam inferir que a hipertensão do avental branco é uma condição que deve ser analisada de maneira distinta em relação a indivíduos normotensos e portadores de hipertensão essencial. / The white coat hypertension is understood as a persistent increase in arterial pressure in the medical office or clinic, while normal blood pressure is observed in any other circumstances. There are several issues regarding the origin, clinical meaning, prognosis and treatment of this condition. Concerning the etiology, our hypothesis is that an endothelial alteration, leading to deficiency either in the production or utilization of endogenous nitric oxide, may constitute a primary factor for the occurrence of white coat hypertension. This study, developed with the population of the city of Dumont São Paulo, Brazil, aims to characterize the participants in relation to demographical factors and metabolic and physiological changes to afterwards identify and compare plasma levels of nitrate product of nitric oxide degradation among the researchs subjects. We selected 109 individuals, from a sample of 441, who were divided in three groups: normotensive (n=58), essential hypertension (n=33) and white coat hypertension (n=18), following arterial pressure measures with oscilometric device and Arterial Pressure Monitoring Exam. Interviews, data measures and laboratory exams were accomplished as to enable the comparison of the variables found between groups. For the statistical treatment, ANOVA and Tukeys test were used. Results were expressed in terms of means ± means standard deviations. The significance level adopted was p<0,05. White coat hypertension prevalence was of 34,1% with predominance of the feminine gender (83,3%), mean age 45,28, most of the participants original from the state of São Paulo (66,7%), white (88,9%), alphabetized (33,3%), married (72,2%) and with family history of cardiovascular diseases (72,2%). Quantification of plasma nitrate showed significant difference between the white coat hypertension group and the normotensive group in comparison to hypertensive patients, with increased levels of serum nitrate in essential hypertension patients. We also found statistically significant difference for corporal mass index, hip/waist ratio, plasma glucose and creatinine, in the comparison between white coat hypertensive and normotensive patients. The distinctions observed between groups and the presence of clinical, demographical and biochemical variations allow us to suggest that the white coat hypertension is a condition which must be analyzed in a distinct way in relation to normotensive and essential hypertension patients.

Neste trabalho foram realizados as sínteses, caracterização e o estudo da reatividade química dos complexos trans-[RuCl(NO)(py)4](PF6)2½H2O e trans-[RuCl(NO)(py)4]Cl23H2O. Os compostos foram isolados e caracterizados através de técnicas espectroscópicas (UV-vis, infravermelho e 1H RMN), eletroquímicas (voltametria cíclica e polarografia de pulso diferencial) e análise elementar. Os dados espectroscópicos, eletroquímicos e os resultados da análise elementar foram consistentes com as fórmulas dos complexos analisados. Foram utilizados como precursores os compostos [RuCl2(DMSO)4], trans-[RuCl2(py)4] e trans-[Ru(NO2)2(py)4]. O composto com NO foi obtido a partir do complexo trans-[Ru(NO2)2(py)4] em ácido clorídrico concentrado e precipitado como sal de PF6-, formando o complexo trans-[RuCl(NO)(py)4](PF6)2½H2O. O trans-[RuCl(NO)(py)4]Cl23H2O foi obtido a partir de uma solução do complexo trans-[RuCl(NO)(py)4](PF6)2.½H2O, adicionando-se uma solução de cloreto de tetrabutilamônio para precipitar o composto na forma de cloreto. Os espectros na região do UV-vis dos complexos trans-[RuCl(NO)(py)4](PF6)2½H2O e trans-[RuCl(NO)(py)4]Cl23H2O apresentaram três bandas nas regiões ao redor de 230 nm, 260nm e 450 nm. Os espectros de infravermelho apresentaram freqüência de estiramento NO entre 1900 e 1925 cm-1, cujo valor variou dependendo das condições em que foram obtidos os espectros. O ciclovoltamograma do composto trans-[RuCl(NO)(py)4](PF6)2½H2O, obtido em solução de acetonitrila, apresentou um processo redox atribuído ao par NO+/0 e um outro processo com E2c = -0,59 V vs Ag/AgCl atribuído à redução do NO0. A atribuição do processo redox com E½ = +0,30 V vs Ag/AgCl foi confirmada através do experimento de espectro eletroquímica na região do infravermelho, onde a banda atribuída ao NO foi diminuindo à medida que o composto ia sendo reduzido. Na espectroeletroquímica na região de UV-vis em acetonitrila foi possível observar uma reação acoplada à redução do composto trans-[RuCl(NO)(py)4](PF6)2½H2O, podendo ser a substituição do NO0 por uma molécula de solvente. O comportamento eletroquímico do composto trans-[RuCl(NO)(py)4]Cl23H2O foi estudado em solução aquosa. O ciclo voltamograma do composto apresentou um processo redox com E½ =+0,13 V vs Ag/AgCl, que é atribuído ao par NO+/0, um processo em +0,25 V e outro em 0,59 V. Dois processos redox dependentes do processo em +0,25 V foram observados nas faixa entre 0,4-0,7 V e podem ser devidos a produtos formados após uma provável saída do Cl- seguido da liberação do NO, como ocorre com o complexo trans-[RuCl(NO)(cyclam)](PF6)2. No estudo da reatividade do ligante NO no complexo trans-[RuCl(NO)(py)4]2+, observou-se que o NO é pouco reativo ao ataque nucleofílico do OH-, apresentando um pKa do equilíbrio entre as espécies nitro e nitrosilo em 11,5 e um Keq = 6,2.104 L2.mol-2. / In this work, the syntheses, characterization and chemical reactivity of trans-[RuCl(NO)(py)4](PF6)2½H2O and trans-[RuCl(NO)(py)4]Cl23H2O were studied. The complexes were isolated and characterizated by elemental analyses UV-vis, infrared and 1H NMR spectroscopies, cyclic voltammetry and spectroelectrochemistry. [RuCl2(DMSO)4], trans-[RuCl2(py)4] and trans-[Ru(NO2)2(py)4] were used as precursor complexes. The compound with NO ligand was obtained from the reaction of trans-[Ru(NO2)2(py)4] with chloridric acidic, and precipitation with PF6-, resulting in trans-[RuCl(NO)(py)4](PF6)2½H2O. The trans-[RuCl(NO)(py)4]Cl23H2O complex was obtained from a trans-[RuCl(NO)(py)4](PF6)2½H2O acetonitrile solution adding a tetrabutylammonium chloride acetonirile solution. The complex was precipitated as a chloride salt. The UV-vis spectra of trans-[RuCl(NO)(py)4](PF6)2½H2O and trans-[RuCl(NO)(py)4]Cl23H2O displayed three bands around the 230 nm, 260 nm and 450 nm regions. The infrared spectra showed the stretching frequencies NO in the 1900-1925 cm-1 range whose values depend on the conditions with which the spectra were recorded. The cyclic voltammetry of the trans-[RuCl(NO)(py)4](PF6)2½H2O in acetonitrile solutions showed a pair of peaks (E½ = +0.30 V vs Ag/AgCl) corresponding to the NO+/0 redox processes, and other process at E2c = -0.59 V vs Ag/AgCl assigned to NO0 reduction. The assignment of the redox processes was confirmed through infrared spectroelectrochemistry, where the band of NO decreases while the compound was reduced. The UV-vis spectroelectrochemistry in acetonitrile solution, showed a coupled reaction following the trans-[RuCl(NO)(py)4]2+ reduction. This coupled reaction is probably the NO substitution by a solvent molecule. The electrochemical behavior of trans-[RuCl(NO)(py)4]Cl23H2O was studied in aqueous solution. The cyclic voltammetry of this complex displayed a pair of peaks (E½ = +0.13 V vs Ag/AgCl) corresponding to NO+/0 redox processes, a process at -0,25 V and other at 0,59 V. Two other couples dependent on the process at 0.25 V, were observed between +0,4 V and +0,7 V. These may be due to products of the Cl- release followed by the NO release, as occurs in trans-[RuCl(NO)(cyclam)](PF6)2. The trans-[RuCl(NO)(py)4]2+ complex showed a low affinity for OH-, with a pKa of the equilibrium between nitro and nitrosyl species at 11.5 and a Keq = 6.2 x 104 L2.mol-2.

A redução eletroquímica de íons complexos do tipo trans-[Ru(NH3)4LNO]2+, foi investigada com o intuito de verificar a possibilidade desses complexos atuarem como doadores de nitroxil (HNO/NO-). Neste trabalho foi dada uma ênfase especial no estudo da espécie em que L = P(OEt)3. Aplicando-se as técnicas de voltametria cíclica e de pulso diferencial, voltametria de onda quadrada e espectroeletroquímica, foi possível verificar que a espécie trans-[Ru(NH3)4P(OEt)3NO]2+ sofre redução monoeletrônica gerando o íon complexo trans-[Ru(NH3)4P(OEt)3NO]+. Foram analisadas as estabilidades relativas das espécies possivelmente formadas, trans- [Ru(NH3)4P(OEt)3NO]+ e trans-[Ru(NH3)4P(OEt)3HNO]2+, baseadas na Teoria de Densidade Funcional (DFT). Após a transferência eletrônica, de acordo com a equação abaixo, a espécie formada sofre isomerização de spin, ocorrendo posteriormente a dissociação do grupo NO O processo apresenta-se como irreversível no espectro voltamétrico devido às restrições de spin. A reação do íon complexo trans-[Ru(NH3)4P(OEt)3NO]3+ com o alfacetoglutarato (Ered = -0,90 V), composto capaz de agir como redutor de dois elétrons por transferência de elétrons de esfera externa, foi monitorada eletroquímica e espectrofotometricamente utilizando a metmioglobina, Mb - Fe(III), captador seletivo de nitroxil. Os resultados obtidos sugerem o complexo supracitado como um possível doador de niroxil. / The electrochemical reduction behavior of the complexes trans-[Ru(NH3)4P(OEt)3NO]2+ was investigated in order to determine the possibility of these complexes to serve as nitroxyl donors (HNO/NO-). Special emphasis was given when L = P(OEt)3. Using cyclic voltammetry and differential pulse, square wave voltammetry and spectroelectrochemistry techniques was possible to infer that the ion complex trans-[Ru(NH3)4P(OEt)3NO]2+ suffers an one electron reduction generating the ion complex trans-[Ru(NH3)4P(OEt)3NO]+. Analyzing the behavior of the cyclic voltammetry spectra, is possible to conclude that the process is irreversible by spin restrictions. The reaction of the ion complex trans-[Ru(NH3)4P(OEt)3NO]+ with the reductor agent alpha-ketoglutarate (Ered = -0,90V), a two electron reductor by outersphere pathway, was monitored by electrochemistry and UV-vis spectroscopy techniques using Mb-Fe(III) as a selective nitroxyl scavenger. The results obtained with these experiments confirm the original purpose of this study.

Many illnesses and infections are exacerbated and/or caused by biofilms. Neisseria gonorrhoeae, the etiologic agent of gonorrhea, is frequently asymptomatic in women, which can lead to persistent infection. Persistent infection can result in pelvic inflammatory disease, tubo-ovarian abscesses, infertility, and ectopic pregnancy. N. gonorrhoeae has been shown to form biofilms over glass, primary and immortalized cervical cells, and during natural cervical infection. Asymptomatic infection occurs in only 1% of infected males, and the infection site is subject to periodic rapid fluid flow, which may limit biofilm formation. Thus, biofilm formation may specifically play an important role in the infection of women and could contribute to the infrequent occurrence of symptoms. Prior to work presented in this dissertation, little was known about biofilm formation by N. gonorrhoeae. Therefore, we elected to compare the transcriptional profiles of biofilms to their planktonic counterparts, to identify genetic pathways involved in biofilm formation and maintenance. We found that 3.8% of the genome was differentially regulated, and that genes involved in anaerobic metabolism and oxidative stress tolerance were up-regulated in biofilm, while genes involved in aerobic metabolism were down-regulated. We determined that expression of aniA , ccp, and norB is required for robust biofilm formation over glass and human cervical cells, and anaerobic respiration occurs in the substratum of gonococcal biofilms. Disruption of the norB gene resulted in severe attenuation of biofilm formation. We determined that the accumulation of nitric oxide (NO) contributes to the phenotype of a norB mutant and can retard biofilm formation when present at sublethal concentrations. However, higher concentrations of NO can enhance biofilm formation in the absence of nitrite. NO enhances biofilm formation in an aniA mutant, but cannot completely restore biofilm formation, suggesting that NO can support anaerobic growth, although nitrite is preferred. We determined that the majority of the genes involved in gonococcal oxidative stress tolerance are required for normal biofilm formation, as mutations in the following genes resulted in biofilm attenuation over cervical cells and/or glass: oxyR, gor, prx, mntABC, trxB, and estD. Overall, biofilm formation may represent an adaptation for coping with the stresses present in the female genitourinary tract.

The atmosphere is a heterogeneous system which is rich in potential chemistry. The processes taking place within this system as well as at the interface of its constituents are of immense importance in understanding how the atmosphere in turn can impact the well-being of all living on the surface of earth. Thus, the heterogeneous chemistry of atmospheric aerosols has since long been subjected to extensive scientific investigation, in view of broadening our understanding of this imperative system. In this study, the heterogeneous interactions of water vapor and gaseous HNO3 on goethite (a-FeOOH), a prominent component of mineral dust aerosol is investigated with quartz crystal microbalance (QCM) measurements and attenuated total reflectance - Fourier transform IR (ATR-FTIR) spectroscopy. Laboratory synthesized goethite samples of varying size (microrods of specific surface area 34 m2/g and nanorods of specific surface area 121 m2/g) were used in order to identify the size dependent interaction of goethite with H2O and HNO3. The study revealed that the exposure of goethite to gas phase H2O and HNO3 results in the uptake of these gases via surface adsorption. Additionally, this novel combined approach of QCM and ATR-FTIR spectroscopy allowed for quantification of the amount of uptake while the spectroscopic data provided information on the speciation of adsorbed products. Thus, with the QCM and spectroscopic data in hand, a precise interpretation of the reactivity as well as its size dependence was sought. In a general sense, the reactivity of a substance is believed to increase with decreasing particle size. The results of this investigation show that in the case of H2O, both microrods and nanorods take up water while the total amount of adsorbed water, when normalized to surface area, is similar for both particle sizes. However, for HNO3, the saturation coverage of total and irreversibly bound HNO3 on microrods was observed to be higher than that on nanorods. With supplementary analysis, this anomalous size effect was attributed to structural features such as the involvement of surface hydroxyl groups in determining the reactivity, which would be subjected to change as a function of particle size. Furthermore, an investigation of the behavior of HNO3 reacted goethite in aqueous media and the uptake of H2O and HNO3 at their mutual presence was carried out such as to better understand the effects of atmospheric processing upon dispersal within the hydrosphere. Further analysis is warranted before arriving at a general conclusion on the size-dependent reactivity of goethite. However, we may argue that goethite containing aerosols may indicate the same pattern of reactivity within the atmosphere as that observed here. Thus, the inference of this investigation proves to be significant in broadening our understanding of this atmosphere as well as the entire biosphere as a whole.

archives@tulane.edu / Experimental stroke in endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) knockout mice showed diverse effects on brain injury. nNOS and eNOS have been shown to uncouple in pathological conditions to produce superoxide. Oxidative stress is believed to be the underlying cause of several cardiovascular diseases including ischemic stroke. However, the role of eNOS and nNOS uncoupling in ischemic stroke is not well studied. Our objective of the study was to determine the effect of eNOS and nNOS inhibition on reactive oxygen species (ROS), NO, viability and mitochondrial bioenergetics in rat brain microvascular endothelial cells (BMECs) and rat cortical neurons following oxygen-glucose deprivation-reoxygenation (OGD/R). We found that non-specific inhibition of NOS in endothelial cells reduced ROS levels in BMECs but increased ROS levels in neurons under normoxia. This suggests that a pool of uncoupled NOS exists in the BMECs whereas the dominant functional NOS in neurons produces NO. We observed increased levels of ROS following OGD/R that is sensitive to NOS inhibition in both BMECs and neurons indicating eNOS and nNOS uncoupling during OGD/R. Furthermore, NOS inhibition reduced mitochondrial respiration while it improved cell survival rate in both BMECs and neurons following OGD/R. Thus, it is possible that decreased mitochondrial respiration in the immediate aftermath (4 hours) of OGD/R could be protective against reoxygenation injury. Moreover, we identified the expression of nNOS in BMECs from rat, human, and mouse. We observed that the nNOS in the BMECs constitutively produces superoxide under physiological conditions instead of NO. In contrast, nNOS in the neurons produces NO and doesn’t contribute to ROS. We also confirmed the nNOS expression and its function in freshly isolated rat brain microvessels. In addition, we developed a novel method to measure mitochondrial respiration in freshly isolated mouse brain microvessels using Seahorse XFe24 Analyzer. We validated the method by demonstrating impaired mitochondrial respiration in cerebral microvessels isolated from old mice compared to young mice. In summary, the present doctoral research investigated the distinct role of NOS isoforms in BMECs and Neurons leading to the identification of novel functional variant of nNOS in BMECs and brain microvessels. / 1 / RAMARAO SVNL

Accurate knowledge of heat transfer to materials in recombining plasmas is needed to improve heat shield designs. A lack of understanding of the chemical component of surface heating motivates the use of conservative assumptions with regards to surface catalysis in the design of thermal protection systems (TPS) that detrimentally impact payload capability. Chemical heating is the release of potential energy from recombining reactive species on the surface to form molecules. For a stable surface interacting with partially-dissociated air, the chemical heating component is due to surface-catalyzed recombination reactions of atomic O and N to produce molecular O2, N2, and NO. Unfortunately, heat flux measurements provide no fundamental information about the surface recombination pathways involved, or how the energy reaches the surface. Rather, they give a total heating rate. This work has taken steps to advance the current poor understanding about the chemical energy transport to and from material surfaces in high-temperature, recombining plasmas. A combination of spatially resolved laser-based diagnostics and emission spectroscopy was used to measure the number densities and gradients of the reactants (N, O), the products (NO, N2) and the energy distribution of recombined molecules (NO, N2) in the boundary layer adjacent to a plasma heated material. Laser excitation can probe individual species by electronic state (atoms) and by electronic, vibrational and rotational states (molecules). Emission can probe the radiative emission for a range of species and electronic, vibrational and rotational states of both atoms and molecules. These measurements of spatial variations in species concentrations through the boundary layer are directly related to near-surface gas-phase chemistry and energy exchange and have provided experimental information that was not currently available. Results provide the initial steps to determine recombination rates and the energy deposited on the surface due to surface catalyzed recombination of atomic nitrogen and oxygen in air plasma.

The large scale nitric acid oxidation of common sugars into their corresponding aldaric acids is being investigated as an important source of potentially useful components for industrial applications such as polymers. This thesis details the development of an Ion Chromatography (IC) method for the analysis of these oxidation mixtures and related samples from the work-up and purification processes. The method was developed for use with a Dionex ICS2000 IC system equipped with an AS11-HC column and utilising suppressed conductivity detection. IC proved to be a useful, versatile and straightforward method of studying the reactions and their products. The detected ions include but are not restricted to the anionic salt forms of: D-Glucaric acid, Xylaric acid, Mannaric acid, D-gluconic acid and both keto forms of the same, D-xylonic acid, D-mannonic acid, glycolic acid, oxalic acid, tartaric acid and tartronic acid. Nitrate from the nitric acid used in the oxidation is often observed. The results compare favorably to GC-MS and HPLC analysis of similar samples. An overview of the theory and operation of the instrument along with the method development and results from application to the oxidation mixtures and related samples are presented. As part of the investigation into the range of utility of IC for studying these reactions, a study was made of the retention behaviour of a large number of simple and low molecular weight (LMW) carboxylic acids eluted by the ion chromatography system in use. The results of this study are included with an explanation of the major factors affecting anion retention on the column

共著者あり。共著者名:Hizawa Nobuyuki, Yamaguchi Etsuro, Jinushi Eisei, Nishimura Masaharu. / Hokkaido University (北海道大学) / 博士 / 医学

<p>Reduksjon av gassbobler i blodbanen: metoder for å forebygge ugunstige effekter av dekompresjon.</p><p>Når en dykker returnerer til overflaten etter dykking, kan det dannes gassbobler i kroppen som følge av overmetning av gasser. Slike gassbobler kan igjen føre til trykkfallsyke, men det gjenstår fremdeles å finne alle mekanismene bak denne sammenhengen. Gassbobler er derimot gode indikatorer på risiko for trykkfallsyke, og den gjennomgående arbeidshypotesen i denne avhandlingen har vært at gassbobler i blodbanen er den bakenforliggende årsaken til alvorlig trykkfallsyke. Det å redusere mengden gassbobler vil dermed øke sikkerheten for dykkeren.</p><p>Avhandlingen består av tre studier som på forskjellige måter forsøker å redusere boblemengden ved trykkreduksjon. Alle arbeidene er gjennomført med bruk av gris som forsøksdyr, og alle dykkene er simulert i trykk-kammer spesielt laget for slike studier. For å måle gassbobler har vi benyttet ultralydavbildning, samt at vi har tatt ut kar for å måle eventuelle funksjonelle endringer i disse i etterkant av dykkene.</p><p>Den første studien demonstrer en ny metode for å redusere gassbobledannelsen ved dekompresjon. Ved kortvarig å øke trykket under pågående trykkreduksjon kan boblemengden signifikant reduseres, resultatene viser at en modell som tar hensyn til bobledannelse beskriver resultatene bedre enn en tradisjonell modell som bare tar hensyn til overmetningen. I den andre studien har vi for første gang vist at gassbobler i blodbanen kan påvirkes medikamentelt også hos store dyr under dekompresjon fra metning. Ved å gi nitrater umiddelbart før dekompresjonen startet, ble mengden gassbobler signifikant redusert sammenlignet med kontrollene som ikke fikk tilført nitrater. Studien åpner veien for videre studier av biokjemiske prosesser involvert i både dannelsen av og effektene av gassbobler. I den siste studien undersøkte vi om en behandlingsprosedyre for trykkfallsyke til bruk når et trykk-kammer ikke er tilgjengelig ville være effektiv om behandlingstrykket ble redusert fra 190 kPa til 160 kPa med pusting av ren oksygen. Vi viste her at trykket var tilstrekkelig for å fjerne boblene etter dykket, men vi forhindret ikke skader på blodkarene.</p><p>Kandidat: Andreas Møllerløkken</p><p>Institutt: Institutt for sirkulasjon og bildediagnostikk</p><p>Veileder: Professor Alf O. Brubakk</p><p>Finansieringskilder: Statoil, Norsk Hydro, Phillips Petroleum Company Norway og Petroleumstilsynet gjennom programmet forskning og utvikling innen dykking, kontraktsnr. 4600002328 med Norsk Undervannsintervensjon (NUI).</p><p>Ovennevnte avhandling er funnet verdig til å forsvares offentlig</p><p>for graden Philosophia Doctor i medisinsk teknologi</p><p>Disputas finner sted i Auditoriet, Medisinsk teknisk forskningssenter</p><p>Tirsdag 15.01.08 , kl. 12.15</p>