A intensidade de pastejo e o uso de fertilizantes nitrogenados podem impactar as características estruturais no dossel forrageiro afetando o ambiente luminoso dentro da vegetação, o que por sua vez pode influenciar a taxa fotossintética das folhas e do dossel, e também a produção de forragem. O objetivo deste trabalho foi descrever e explicar os efeitos da altura do dossel mantida constante e da taxa de crescimento aplicada como dose de nitrogênio (N) do capim Mulato II (Brachiaria brizantha × B. decumbens × B. ruziziensis) sob lotação contínua e taxa de lotação variável sobre as características produtivas e morfofisiológicas e a participação de diferentes categorias de folhas na composição do índice de área foliar (IAF) e na fotossíntese do dossel. O estudo foi conduzido na ESALQ/USP em Piracicaba-SP durante dois verões agrostológicos. O delineamento experimental foi o de blocos completos casualizados, com arranjo fatorial combinando três alturas do dossel (10, 25 e 40 cm) e duas taxas de crescimento geradas por doses de N (50 e 250 kg ha-1 ano-1), em três repetições. As variáveis estudadas incluíram: acúmulo total anual de forragem (ATF), IAF, interceptação de luz (IL), taxas de fotossíntese foliar e do dossel e contribuição relativa de diferentes categorias de folhas no IAF e na fotossíntese do dossel. O ATF aumentou linearmente com a altura do dossel (de 8560 para 13600 kg MS ha-1 ano-1). Aumentos também foram observados para IAF, IL, taxa de fotossíntese do dossel e contribuição das folhas maduras (MAD) no IAF e das folhas mais jovens completamente expandidas (JCE) na fotossíntese do dossel. A contribuição das folhas em expansão (EXP) e das folhas JCE no IAF, a taxa de fotossíntese das folhas EXP e MAD e a contribuição relativa das folhas EXP na fotossíntese do dossel aumentaram com a diminuição da altura do dossel. A aplicação de 250 kg N ha-1 ano-1 resultou em aumento de 137% no ATF em relação à taxa de 50 kg ha-1 ano-1. A maior dose de N resultou em aumento significativo no IAF, IL, taxa de fotossíntese de todas as categorias de folhas, fotossíntese do dossel e contribuição das folhas JCE na fotossíntese do dossel, embora a contribuição das folhas EXP na fotossíntese do dossel tenha sido maior na menor dose de N. A contribuição das folhas MAD na fotossíntese do dossel foi maior quando o dossel foi mantido mais alto e também com maior dose de N na altura de 10 cm. Nos dosséis de 25 cm a proporção de folhas MAD foi maior na menor dose de N e não diferiu entre doses de N quando mantido a 40 cm. Embora dosséis mantidos mais baixos apresentem maior proporção de folhas mais jovens (EXP e JCE) que são as que apresentam maiores taxas fotossintéticas, a fotossíntese do dossel e o ATF foram maiores nos dosséis mais altos. A aplicação de 250 kg N ha-1 ano-1 resulta em aumento na fotossíntese do dossel como consequência do aumento do IAF e da taxa de fotossíntese foliar, resultando em aumento no ATF. / Grazing intensity and nitrogen fertilization can impact the structural characteristics of the canopy affecting the light environment within the vegetation, which in turn may affect leaf and canopy photosynthetic rates, and also forage production. The objective of this study was to describe and explain the effects of canopy height kept constant and growth rate applied as N rate of Mulato II brachiariagrass (Brachiaria brizantha × B. decumbens × B. ruziziensis) under continuous stocking and variable stocking rate, on the productive and morpho-physiological characteristics, and the relative contribution of different leaf categories to the leaf area index (LAI) and to canopy photosynthesis. A field trial was conducted at ESALQ/USP in Piracicaba, SP, Brazil, during two summer growing seasons. A randomized complete block design was used, with a factorial arrangement combining three canopy heights (10, 25, and 40 cm) and two growth rates imposed by N rates (50 and 250 kg ha-1 yr-1), with three replications. The variables studied included: total annual forage accumulation (TFA), LAI, light interception (LI), leaf and canopy photosynthetic rates, and the relative contribution of different leaf categories to the LAI and to canopy photosynthesis. There was a linear increase in TFA with increased canopy height (from 8560 to 13600 kg DM ha-1 yr-1). The LAI, LI, canopy photosynthesis rates, the relative contribution of mature leaves (MAT) to the LAI, and relative contribution of the youngest fully-expanded leaves (YFE) to canopy photosynthesis also increased with canopy height. The relative contribution of expanding leaves (EXP) and YFE to the LAI, leaf photosynthesis of EXP and MAD leaves, and relative contribution of EXP leaves to canopy photosynthesis increased with the reduction in canopy height. The use of a greater N rate (250 kg N ha-1 yr-1) increased ATF by 137%. The application of more N (250 kg N ha-1 yr-1) also increased LAI, LI, photosynthetic rates of all leaf categories, canopy photosynthesis, and relative contribution of YFE leaves to canopy photosynthesis, but the relative contribution of EXP leaves to canopy photosynthesis was greater in the lesser N rate (50 kg N ha-1 yr-1). The relative contribution of MAT leaves to canopy photosynthesis was greater in taller canopies, and also in canopies maintained at 10 cm fertilized with 250 kg N ha-1 yr-1. The canopies maintained at 25 cm showed greater proportion of MAD leaves in the lesser N rate, and was similar in 40-cm canopies for both N rates. Although canopies kept at 10 cm showed greater relative proportion of younger leaves (EXP and YFE), which are those with greater photosynthetic rates, canopy photosynthesis and the TFA were greater in taller canopies. The application of 250 kg N ha-1 yr-1 results in increased canopy photosynthesis, due to increased LAI and leaf photosynthetic rate, resulting in increased TFA.

In this diploma thesis the impacts of both nitrogen fertilization and drought on the content of proteins of the gliadin fraction in case of a winter wheat variety Tiguan were observed. Selected samples were cultivated at two locations in a total duration of two years. Two different conditions were selected: one without the nitrogen fertilization (0 kg N/ ha) and the other with the nitrogen fertilization in 140 kg N/ ha concentration. Second observed factor was the drought which was ensured by roofs above the crops and their cultivation in common climatic environment. Gliadin fraction was separated by the A-PAGE method and the protein quantification carried out by a computer densitometry. Such nitrogen fertilization caused a significant increase of gliadins, especially -1,2 and -5 fractions. Stress induced by the drought caused an increase of gliadin content compared to the control sample, particularly in case of - and - fractions. The most important factor influencing the gliadin content in grains was therefore the nitrogen fertilization in a dry environment. The observed increase of gliadin content was lower in case of the sample which was fertilized in a humid environment than in case of the sample which was fertilized in the dry one.

Resumo: O objetivo deste trabalho foi avaliar respostas do capim-tifton 85 a doses de nitrogênio (N) associadas a doses e fontes de boro (B). Em casa de vegetação, conduziu-se um experimento fatorial 3 ' 3 ' 3 {três doses de N (0, 100 e 200 mg kg-1), três doses de B (0, 1,3 e 2,6 mg kg-1) e três fontes de B [colemanita fundida ao termofosfato (CF), colemanita em mistura com termofosfato (CM) e ácido bórico (H3BO3)]} em delineamento inteiramente ao acaso. As plantas cresceram em vasos preenchidos com amostra de um Latossolo Vermelho distrófico. Elas foram avaliadas em três ciclos de crescimento. O N foi aplicado no início de cada ciclo e o B apenas antes do plantio. A aplicação de N aumentou a massa seca da parte aérea e o número de perfilhos do capim nos três ciclos. A aplicação de B, isolada ou associada à de N, não teve influência nesses atributos, embora o solo tivesse baixo teor de B disponível. O suprimento de N aumentou também os teores de carboidratos de reserva na raiz da planta nos dois ciclos em que foram avaliados (primeiro e terceiro). O suprimento de B aumentou o teor desses carboidratos na base do caule no primeiro ciclo. Os fornecimentos de N e B aumentaram seus respectivos teores na parte aérea do capim no três ciclos, exceto no caso do teor de B no terceiro ciclo. Todas as fontes de B aumentaram o teor de B disponível no solo, mas a CM e o H3BO3 aumentaram mais do que a CF. O efeito mais evidente da aplicação conjunta de N e B ocorreu no acúmulo de B na parte aérea da planta, aumentando mais com a combinação das duas aplicações. O teor inicial de B no solo (0,15 mg dm-3) foi suficiente para atender as necessidades do capim-tifton 85 nos três ciclos de crescimento. / Abstract: The objective of this work was to evaluate responses of Tifton 85 bermudagrass to nitrogen (N) rates associated with boron (B) rates and sources. In greenhouse, it was carried out a factorial experiment 3 ' 3 ' 3 {three N rates (0, 100 and 200 mg kg-1), three B rates (0, 1.3 and 2.6 mg kg-1) and three B sources [colemanite fused to termophosphate (CF), colemanite in mixture with termophosphate (CM) and boric acid (H3BO3)]} in complete randomized design. The plants grew in pots filled with sample of a Typic Haplustox. They were evaluated during three growth cycles. The N was applied at the beginning of each cycle and the B just before the planting. The N application increased both top dry mass and tiller number of the grass in the three cycles. The B application, isolated or associated with N application, did not have influence on these attributes, though the soil had low available B content. The N supply increased reserve carbohydrates contents in plant root in the two cycles in which they were evaluated (first and third). The B supply increased the content of these carbohydrates in the stem base in the first cycle. The N and B furnishings increased their respective contents in top grass in the three cycles, except in the case of the B content in the third cycle. All the sources increased the available B content in soil, but CM and H3BO3 increased more than CF. The most evident effect of the N and B applications together occurred in the B accumulation in top plant, increasing more with the combination of the two applications. The initial B content in soil (0.15 mg dm-3) was enough to attend necessities of Tifton 85 bermudagrass in the three growth cycles. / Orientador: Edson Luiz Mendes Coutinho / Coorientador: Edemo João Fernandes / Banca: Mara Cristina Pessôa da Cruz / Banca: Cassio Hamilton Abreu Junior / Banca: Takashi Muraoka / Banca: Renato de Mello Prado / Doutor

Resumo: Os tecidos vegetais tendem a ficar mais tenros em sistemas de produção onde maior fertilização com nitrogênio (N) é exigida, favorecendo o auto-sombreamento das plantas; entretanto, com aplicação de silício (Si), há melhoria na arquitetura foliar, reduzindo tal fenômeno. Objetivou-se, com este estudo, avaliar os efeitos da adubação nitrogenada e sua interação com a adição de Si na forma de escória de siderurgia nos atributos químicos do solo, na nutrição e na produtividade da cana-planta. O experimento foi instalado em condições de campo no município de Jaboticabal, SP, Brasil. Os tratamentos foram dispostos em esquema fatorial 5x2, sendo cinco doses de N (0, 30, 60, 90 e 120 kg ha-1) com Si (escória de siderurgia) e sem adição de Si (calcário), em delineamento em blocos ao acaso, com quatro repetições. Os atributos químicos do solo não diferiram com a aplicação de calcário ou de escória após 99 dias da aplicação dos materiais corretivos tampouco não houve mobilidade das bases no perfil do solo. A adubação nitrogenada com ou sem adição de Si não afetou as variáveis de desenvolvimento da cana-planta. Os maiores acúmulos de N, P, K, Mg, S, Cu e Zn ocorreram no colmo, ao passo que os maiores acúmulos de Ca, B, Fe, Mn e Si apresentaram-se nas folhas. Os elementos mais extraídos pela cana-planta (variedade RB855156) foram Si, K e N. Houve efeito da adubação nitrogenada na produtividade da cana-planta quando associada à adição de Si na forma de escória, ocorrendo aumento de 15 t ha-1 na produção de colmos industrializáveis. O uso da adubação nitrogenada incrementou o teor de sacarose e fibra de forma linear, entretanto, não houve efeito da escória nas variáveis tecnológicas da cana-planta. De modo geral, a adubação nitrogenada associada à adição de Si na forma de escória mostra-se adequada ao cultivo da cana-planta / Abstract: In cases where more N fertilization is applied, plant tissues are tender and self-shading of the plants may result. Moreover, Si is important because it promotes improved leaf architecture and reduces self-shading. The objective of this study was to evaluate the effect of nitrogen with addition of Si as slag in soil chemical properties, nutrition and cane plant yield. The experiment was carried out in Jaboticabal, Sao Paulo State, Brazil with five N rates: 0, 30, 60, 90, and 120 kg ha-1 with Si (slag) and without Si (lime), in a randomized block with four replications. The soil chemical properties did not differ with the application of lime or slag in 99 days after application neither any mobility of bases in the soil profile was verified. Nitrogen fertilization with or without addition of Si did not affect the growth parameters of the cane plant. Accumulation of N, P, K, Mg, S, Cu and Zn mostly occurred in the stalk, while the highest Ca, B, Fe, Mn and Si accumulation were found in the leaves. Si, K and N were the most elements extracted by the cane plant (variety RB855156). No significant effects of N fertilization on cane plant yield; however, when associated with Si as slag, there was an increase of 15 t ha-1 of stalks. Nitrogen fertilizer increased the sucrose content and fiber linearly, however, no effect of adding slag in the technological variables of the cane plant. In general, nitrogen associated with Si added in the form of slag proved to be adequate for the cultivation of cane plant / Orientador: Renato de Mello Prado / Coorientador: Wanderley José de Melo / Banca: José Lavres Junior / Banca: Francisco Maximino Fernandes / Banca: Edson Luiz Mendes Coutinho / Banca: Miguel Ângelo Mutton / Doutor

Nitrogen (N) is a central nutrient in cropping systems. However, a considerable N fraction is lost through runoffs and leaching with detrimental consequences for environment and controversial effects on human health. Increasing the plant N uptake by optimizing the degree of root branching for exploring a larger soil volume in search of the mobile nitrate resource may contribute to limit soil leaching and subsequently to rely more efficiently on the soil mineralization and fertilizer inputs. Rapeseed (Brassica napus L.) is a major oil crop that highly depends on N fertilization. This doctoral thesis aims at exploring the diversity of root morphology in recently selected cultivars and in a large set of rapeseed inbred lines, and at understanding the genetic control on root morphology and how it is impacted by N nutrition.Firstly, a panel of twenty-eight European recently selected cultivars of winter oilseed rape were tested in laboratory and field conditions. Upon hydroponic culture, these hybrids showed a great diversity for biomass production and root morphological traits. Differences in root and shoot dry biomasses and lateral root length were mainly explained by the genotype, while differences in primary root length by the nutrition. The cultivars were tested in a pluriannual field trial. The observed variation for yield and seed quality traits attributed to the genotype was more important than the year or the genotype x year interaction effects. The total root length measured in laboratory could predict the proportion of nitrogen taken-up from the field and reallocated to the seeds. The genetic interrelationship between cultivars, established with polymorphic markers, indicated a very narrow genetic base. Positive correlations were found between the genetic distance measures, root morphological trait distances during nitrogen depletion conditions, and agronomic performance. Secondly, three cultivars previously selected from a root morphology screen at a young developmental stage were field tested with two nitrogen applications. The purpose was to examine the relationship between root morphology and Nitrogen Uptake Efficiency (NUpE) and to test the predictiveness of canopy optical indices for seed quality and yield. A tube-rhizotron system was used to incorporate below-ground root growth information. One-meter length clear tubes were installed in soil at an angle of 45°. The root development was followed with a camera at key growth stages in autumn (leaf development) and spring (stem elongation and flowering). Autumn was a critical time window to observe the root development and exploration in deeper horizons (36-48 cm) was faster without any fertilization treatment. Analysis of the rhizotron images was challenging and it was not possible to clearly discriminate between cultivars. Canopy reflectance and leaf optical indices were measured with proximal sensors. The Normalized Difference Vegetation Index (NDVI) was a positive indicator of biomass and seed yield while the Nitrogen Balance Index (NBI) was a positive indicator of above-ground biomass N concentration at flowering and seed N concentration at harvest.Thirdly, the natural variability offered by a diversity set of 392 inbred lines was screened to apprehend the genetic control of root morphology in rapeseed and how it is impacted by nitrogen nutrition. Seedlings grew hydroponically with low (0.2 mM) or elevated (5 mM) nitrate supplies. Low nitrate supply triggered the primary root and lateral root growth, while elevated supply promoted shoot biomass production. A considerable variation degree in the root morphological traits was observed across the diversity set, and there was no trade-off between abundant lateral root branching and shoot biomass production. Root traits were mainly dependent on the genotype and highly heritable. A genome wide association study identified some genomic regions associated with biomass production and root morphological traits. A total of fifty-nine QTLs were identified and thirty of them were integrated into seven clusters on chromosomes A01 and C07. Some candidate genes were identified with Arabidopsis orthologs related to root growth and development, nitrogen nutrition or hormone regulation.This study provides promising routes for redesigning the root system architecture by uncovering nitrogen-interactive genomic regions shaping root morphology. A perspective is to develop genetic markers associated with root morphological traits that could be used for assisted breeding. / Doctorat en Sciences agronomiques et ingénierie biologique / info:eu-repo/semantics/nonPublished

Dans un contexte de réduction des impacts des pratiques agricoles sur la santé humaine et sur les écosystèmes, il est nécessaire de bien prendre en compte la volatilisation d’ammoniac (NH3) dans les inventaires d’émissions, la compréhension du devenir de l’azote après fertilisation et la modélisation de la qualité de l’air. Etant donné le poids de la fertilisation azotée (55%) dans le total des émissions nationales de NH3, cette dernière constitue un levier important pour réduire les émissions de NH3. Les inventaires nationaux actuels, basés sur l’utilisation de facteurs d’émission par défaut, souffrent d’un manque de description spatiale et temporelle, rendant difficile l’élaboration des politiques de réduction efficaces des émissions. Par ailleurs, même s’il existe à l’heure actuelle des modèles qui simulent de manière globale le devenir de l’azote sur le cycle de la culture, la volatilisation de NH3 n’est pas toujours prise en compte, et si elle l’est, les modules de volatilisation sont souvent relativement frustres et n’ont été que partiellement validés. Il existe pourtant des modèles dédiés exclusivement à la volatilisation de NH3 au champ, mais leurs exigences en données et paramètres d’entrée et leur temps de calcul limitent leur utilisation à grande échelle (exemple, échelle nationale…) sur de nombreux sites géographiques pendant plusieurs années, ainsi que leur intégration dans des modèles opérationnels de prédiction de la qualité de l’air, ou des outils d’aide à la décision ou d’évaluation environnementale en termes de fertilisation azotée.Cette thèse propose des nouveaux outils. Le premier outil, CADASTRE_NH3, permet de décrire et d’analyser la variabilité spatio-temporelle des émissions de NH3 liées à la fertilisation azotée. Il couple le modèle de processus Volt’Air avec des bases de données géo-référencées sur les facteurs agro-pédoclimatiques à l’échelle nationale. Cette approche d’inventaires des émissions a montré sa capacité à capturer les variabilités spatio-temporelles de l’utilisation d’azote et des émissions de NH3 qui en résultent, et à prendre en compte l’effet des interactions des facteurs pédologiques et/ou climatiques sur les émissions. La confrontation des résultats de l’outil CADASTRE_NH3 avec les inventaires officiels français montrent de fortes convergences en ce qui concerne les quantités d’azote utilisé et les émissions de NH3 en France pour l’année 2005-06, mais aussi des divergences notamment pour le cas des produits résiduaires organiques. Le deuxième type d’outils correspond aux méta-modèles dérivés de Volt'Air pour prédire les taux d’émissions de NH3 après application d’engrais azotés. Ces méta-modèles présentent de nombreux avantages pratiques du fait de leur simplicité et opérationnalité. Ils ont des potentiels d’utilisation prometteurs pour accompagner la prise de décision en terme de conditions d’utilisation des engrais et également pour appuyer les politiques de réduction des émissions à travers par exemple la réalisation des tests de scénarios.Il serait cependant intéressant de confronter les outils développés dans le cadre de cette thèse avec des données expérimentales pour évaluer leur performance respective et valider l’ensemble de nos approches. Il serait également intéressant de développer des méta-modèles dynamiques dérivés du modèle Volt’Air capables de décrire la dynamique des flux de NH3 liés à l’application des engrais azotés pour être intégrés comme modules simples de volatilisation de NH3 dans des modèles agronomiques et atmosphériques. / In a context of reducing the impacts of agricultural practices on human health andecosystems, it is necessary to better account for ammonia (NH3) volatilization in the inventories of NH3 emissions, the understanding of the nitrogen behavior after fertilization and the modeling of air quality.Given the considerable weight of nitrogen fertilizer (55%) in the total national NH3 emissions, nitrogen fertilization is an important lever for reducing NH3 emissions. Current national inventories are based on the use of default emission factors (EF) and suffer from a lack of fine spatial and temporal NH3 emissions descriptions making it difficult to develop effective emission reduction policies. Moreover, even if there are currently models that globally simulate the fate of nitrogen on the crop cycle, their do not always take into account the volatilization of NH3, and if it is, the volatilization modules are often very frustrating and have not been validated. There are models dedicated exclusively to the volatilization of NH3 in the field, but their requirements for data and input parameters and their calculation time limit their large-scale use (e.g, at national scale) in many geographical locations for several years as well as their integration into airquality prediction models or decision support or environmental assessment tools in terms of nitrogen fertilization.This thesis proposes new tools. The first tool, CADASTRE_NH3 makes it possible to describe and analyze the spatio-temporal variability of NH3 emissions from nitrogen fertilization. It combines the Volt'Air process-based model with geo-referenced databases on agro-soil-meteorological factors at the national level. This approach has demonstrated its ability to capture the spatio-temporal variability ofnitrogen use and resulting NH3 emissions, and to take into account the effect of soil and climate factor interactions on emissions. The comparison of CADASTRE_NH3 results with the official French inventories shows strong convergences regarding the quantities of nitrogen used and NH3 emissions in France for the year 2005-06, but also divergences especially for the case of organic waste products. Thesecond type of tool corresponds to meta-models derived from Volt'Air for predicting NH3 emission rates after nitrogen fertilizer applications. These meta-models have many practical advantages because of their simplicity and operability. They have promising potential uses to support decision-making in terms of fertilizer use conditions and also to support emission reduction policies through, for example, scenario testing.However, it would be interesting to compare the tools developed in this thesis with experimental data to evaluate their respective performance and validate all our approaches. It would also be interesting to develop dynamic meta-models of Volt'Air that can describe the dynamics of NH3 fluxes related to nitrogen fertilizer applications and to be integrated as simple modules of NH3 volatilization in agronomic and atmospheric models.

Soybean (Glycine max [L.] Merr.) has become one of the main agricultural grain crops produced in the United States. Soybean production continues to increase in high-yield environments throughout the U.S. New innovations are required to sustain gains in soybean yield potential. Field experiments were conducted at the Delta Research and Extension Center in Stoneville, MS in 2014 and 2015 to evaluate soybean aboveground biomass and grain yield response to supplemental N fertilization in a high-yielding environment on two soil textures commonly cropped to soybean in Mississippi. Greenhouse studies were conducted in 2016 at the Delta Research and Extension Center in Stoneville, MS to evaluate the influence of supplemental N fertilization on nodule formation and belowground biomass of soybean on two soil textures commonly cropped to soybean in Mississippi.

En el centro-este del país, el cultivo de trigo y la ganadería forman una parte importante del ingreso de las explotaciones agropecuarias. La producción ganadera se basa en el uso de recursos forrajeros perennes y cultivos forrajeros anuales de uso invernal. Los verdeos invernales son integrantes indispensables de la cadena forrajera de la invernada o el tambo en esta región. Teniendo en cuenta la magnitud de la superficie destinada a trigo, es importante considerar que, además de la producción de grano para cosecha, el forraje verde producido puede ser de utilidad simultáneamente para la producción bovina. La denominación trigo doble proposito (TDP) hace referencia a la utilización de este cultivo para un doble objetivo: la alimentación de animales a pastoreo durante el estadio de crecimiento vegetativo y la producción de grano. Así, el TDP es una alternativa forrajera en los sistemas mixtos agrícolo-ganaderos. La importancia radica en que en este período invernal existe una disminución en la oferta de forraje y, por otro lado, se incrementa la competencia por el uso del suelo con cultivos exclusivamente para grano. De esta manera, el uso de TDP puede generar una mayor eficiencia en la utilización de los recursos disponibles. En nuestro país es muy acotada la información referente al impacto producido por la fertilización nitrogenada sobre la composición nutricional de los verdeos de invierno, en especial los TDP. Para investigar este aspecto, en los años 2000 y 2001 se sembró TDP cultivar Prointa Super en dos sitios experimentales localizados en la provincia de Buenos Aires, pertenecientes a la Estación Experimental Coronel Suárez del Ministerio de Asuntos Agrarios y de la Producción: Pasman (38 21LS y 62 08LO) y Cabildo (38 25 LS y 61 42 LO). Los suelos son Hapludol típico con diferencias de textura y variación en el contenido de materia orgánica (Pasman: 4,4 %; Cabildo: 2,1%). A las parcelas se les aplicó distintas dosis de nitrógeno en forma de urea (0, 40, 80, 120, 160 kg/ha), con un diseño experimental de bloques al azar (n = 4). Se realizaron dos cortes por año, coincidentes con distintos momentos del ciclo de crecimiento del cultivo, el primer corte se realizó cuando el volumen de materia verde fue suficiente para un pastoreo. En el caso del segundo corte, correspondió antes de la emergencia del primer entrenudo (evento que marca el inicio del estado reproductivo de la planta). En cada corte se valuó la producción de forraje, tomándose muestras en cada corte para la determinación de materia seca (MS), proteína bruta (PB), proteína soluble (PS), carbohidratos no estructurales solubles (CNES), fibra detergente neutra (FDN), fibra detergente ácida (FDA), lignina (L) y minerales. Los datos fueron sometidos a análisis de varianza con un diseño factorial de 5 x 2 x 2. Con algunas variaciones entre localidades y años, la fertilización nitrogenada incrementó linealmente la producción de forraje y grano. Con respecto a la composición nutricional del TDP, la fertilización disminuyó linealmente la concentración de MS (P<0,01), como así también hubo una ligera disminución de la concentración de FDN (P<0,05), pero no tuvo un efecto marcado sobre el porcentaje de FDA ni de lignina. No hubo efecto sobre la digestibilidad de MS (DMS). La fertilización nitrogenada disminuyó el contenido de CNES sólo en el año 2000. Hubo un efecto lineal ascendente muy marcado sobre la concentración de PB y PS (P<0,01) con el incremento de la dosis de urea. La fertilización nitrogenada incrementó la relación PB/CNES y PS/CNES (P<0,01). Sin embargo, el primer corte (C1) fue mayor al segundo corte (C2) en el año 2001 (P<0,01). Con respecto a los macrominerales el efecto más significativo de la fertilización nitrogenada fue sobre el contenido de K, el que aumentó linealmente con la dosis de urea (P<0,05). A modo de conclusión, la fertilización nitrogenada tuvo un efecto importante sobre la producción de forraje y grano en TDP. Aunque no afectó la DMS, la disminución de la concentración de MS y el incremento en la PB y la PS podrían impactar negativamente sobre la productividad animal. Este aspecto amerita posteriores investigaciones.

Der Pendelsensor “Crop-Meter” misst indirekt die oberirdische Biomasse von Getreide, um Stickstoffdünger (N) teilflächenspezifisch auf heterogenen Ackerflächen auszubringen. In On-Farm-Versuchen wurde eine variable N-Düngung mit „Crop-Meter“ gegenüber der praxisüblichen, einheitlichen N-Düngung in Wintergetreide getestet. Im Gegensatz zur klassischen Anlage von Parzellenversuchen war eine Bestandsheterogenität zum Sensoreinsatz im Getreide ausdrücklich gefordert. Prüfmerkmal war der Kornertrag. Auf Grund der georeferenzierten Ertragskartierung lagen die somit pseudo-wiederholten Daten vermutlich räumlich autokorreliert vor. Daher wurden zwei Ansätze zur statistischen Analyse gewählt. Im ersten Ansatz wurden die Ertragsdaten auf Basis der Biomasse post-stratifiziert, um die Biomassevariabilität einzugrenzen. In jedem Stratum wurden Autokorrelationsmodelle für die Residuen (räumliche Modelle) mit der Annahme nicht korrelierter Residuen (Nullmodell) verglichen. Stets wurden räumliche Modelle als beste statistische Modelle ausgewählt. Im Gegensatz zu den Nullmodellen waren die Ertragsdifferenzen räumlicher Modelle sehr häufig nicht signifikant. Dieses Ergebnis wurde auch durch verschiedene Stratifikationen und N-Einsparungen nicht verändert. Im zweiten Ansatz wurde jeweils der gesamte Ertragsdatensatz eines Versuchs zusätzlich zu den räumlichen Modellen mit Kovariablen (Trendmodelle) modelliert. Für jeden Versuch wurden individuell verschiedene Kombinationen aus Trend- und räumlichen Modellen selektiert. Die meisten Mittelwertdifferenzen waren trotz N-Reduktion in den variablen Varianten nicht signifikant (7…24 kg N ha-1). Diese Ansätze bilden eine Grundlage zur Ableitung fundierter Auswertungsmethoden von On-Farm-Versuchen mit georeferenzierten Daten. / A pendulum sensor (Crop-Meter) measures cereal aboveground biomass in order to apply nitrogen (N) fertilizers site-specifically on heterogeneous agricultural fields. In on-farm trials, common N application practice (constant) was compared to site-specific (variable) N application with the sensor to assess grain yields as response. Unlike the classic design of small-plot trials, cereal crop heterogeneity was explicitly desired to use the Crop-Meter. Simultaneously, spatial yield data provided pseudo-replications and possible autocorrelation. Therefore, two approaches were selected for statistical analysis. In the first approach, yield data were post-stratified on the basis of biomass measures to confine total biomass variability. For each stratum, spatial co-variance structures were assumed for residuals (spatial models) opposed to the assumption of non-correlated residuals (null model). Spatial models were selected as best statistical models. Very often, yield differences were not significant when selecting spatial models in contrast to null models. However, this was not due to different stratifications and stratum-dependent N savings. In the second approach, trend models were fitted for total yield datasets with covariates in addition to spatial models. For each on-farm trial, distinct combinations of trend and spatial models were selected. Most mean differences were not significant despite site-specific N reduction (7…24 kg N ha-1). These approaches provide basics to derive profound methods for analysis of on-farm trials with spatial data.

Die Anwendung von Biokohlen in der Landwirtschaft gewinnt durch die positiven Aspekte der Kohlenstoffsequestrierung, Bodenverbesserung und eines erhöhten Pflanzenwachstums in den letzten Jahren an Bedeutung. Deshalb geht die vorliegende Arbeit den Fragen nach, welche Wirkungen unterschiedliche Biokohlen in Kombination mit oder ohne Gärrest und / oder Stickstoffdünger auf die Bodenchemie, Bodenbiologie und Wachstum, Entwicklung, Ertrag, Ertragsstruktur, Nährstoffe sowie Qualität von Nutzpflanzen haben. Außerdem wurden die Effekte unterschiedlicher Biokohlen auf die Wurzelmorphologie von Sommerweizen quantifiziert. Eine weitere Frage war, inwiefern Biokohlen Stickstoffquellen sorbieren. Es wurden ein Feldversuch und mehrere Gefäßversuche durchgeführt, um die Änderungen der Bodenchemie, den Einfluss auf die Bodenbiologie am Beispiel von Collembolen und die Pflanzenparameter zu bestimmen. Für die Wurzeluntersuchungen wurden Rhizoboxversuche durchgeführt und zur Quantifizierung der Stickstoffsorption ein in vitro Versuch angelegt. Die Ergebnisse der Feld- und Gefäßuntersuchungen zeigten, dass die Biokohlen die Bodeneigenschaften positiv beeinflussten. Die Biokohlen nahmen keinen konsistenten Einfluss auf die Erträge von Kulturpflanzen. Die Nährstoffgehalte der Kulturpflanzen konnten zum Teil positiv beeinflusst werden. Die Collembolenabundanzen zeigten sowohl in der Feldstudie als auch im Gefäßversuch keine signifikanten Unterschiede zwischen den getesteten Biokohlen. Hohe Mengen der fermentierten HTC-Biokohle führten zu negativen Wirkungen auf die Collembolenabundanz im Gefäßversuch. Die Rhizoboxversuche zeigten einen positiven Einfluss der Pyro-Biokohle auf die oberirdische und unterirdische Trockenmasse sowie die Wurzelmorphologie von Sommerweizen. Unterschiedliche Stickstoffquellen wurden von der HTC-Biokohle stärker sorbiert als von der Pyro-Biokohle. Generell lässt sich aus den differenzierten Wirkungen der Biokohlen weitere Forschung mit dem Fokus Boden ableiten. / Over the past few years the use of biochars in agriculture has gained more importance due to positive effects on carbon sequestration, soil improvement and increased plant growth. Therefore, the present work pursues the question which effects different biochars have in combination with or without digestate and / or nitrogen fertilizer on soil chemistry, soil biology and growth, development, yield, yield components, nutrients and quality of crops. In addition, the effects of different biochars on root morphology of spring wheat were quantified. Further to elucidate potential mechanisms underlying biochar effects on crops it was studied how biochars sorb various nitrogen sources. A field test and several pot experiments were conducted to determine the changes in soil chemistry, the impact on soil-dwelling Collembola and plant parameters. For the root studies rhizobox experiments were performed. To quantify the nitrogen sorption of biochars an in vitro experiment was conducted. The results of the field test and pot experiments showed that biochars positively affected soil chemical characteristics. However, yields of crops were not consistently affected by biochars. The nutrient content of crops were positively influenced to some extent. The abundance of Collembola was not significantly influenced by the biochars tested in the field study and in the pot experiment. High amounts of fermented HTC-biochar had negative effects on the abundance of Collembola in the pot experiment. Rhizobox experiments showed a positive influence of Pyro-biochar on the aboveground dry matter, belowground dry matter and root morphology (e.g. root length) of spring wheat. Amount and reversibility of nitrogen sorption was dependent on the type of biochar with HTC-biochar sorbing more nitrogen than Pyro- biochar. Generally it can be concluded that because of the different effects of biochars further research with a focus on soil is needed.