The olive (Olea europaea L.) is one of the most ancient fruit trees and has been cultivated for its oil in the Mediterranean area for thousands of years. Today, the consumption of olive oil and table olives is increasing both in traditional producing countries and the entire world. Most olive cultivars are self-incompatible and do not produce a commercial yield after self pollination. In this thesis, inflorescence architecture and sexual compatibility relationships of some olive cultivars, and gene expression in olive pistils during flowering were studied. To study the inflorescence architecture of olive, 45 inflorescences in each of the cultivars Manzanillo, Mission, and Frantoio were checked every morning from flower opening to petal fall. The flower position on the inflorescence had a highly significant effect on the opening day in all cultivars. Terminal flowers and the flowers located on the primary branches opened earlier than flowers located on the secondary branches. Flower position also had a highly significant effect on gender in Manzanillo and Mission. In Manzanillo, the secondary branches had fewer perfect flowers than the primary branches. In Mission, the secondary branches had no perfect flowers at all. In Manzanillo, perfect flowers had significantly longer petal persistence than staminate flowers. To study flower competition within the inflorescence, the distal halves, on which the flowers tend to be perfect, of 120 inflorescences in three trees of Manzanillo were removed about one month before full bloom. This resulted in a highly significant increase in the percentage of perfect flowers on the proximal halves. The effects of shoot orientation and inflorescence location on inflorescence characteristics in the cultivars Frantoio, Kalamata, and Koroneiki were also studied. For each cultivar, inflorescence characteristics in three sections of shoots (top, middle, and base) and four sides of the three selected trees (north, south, east, and west) were recorded. The statistical analysis showed that basal inflorescences were shorter and with fewer flowers but with the same percentage of perfect flowers. Shoot orientation did not have any influence on these characteristics in any of the cultivars. Sexual compatibility was assessed using two methods. In the first method, controlled crossings were performed in the cultivars Frantoio, Koroneiki, and Kalamata. The pistils were harvested one week after hand pollination and stained with 0.1% aniline blue. The styles and ovules were separated, mounted in 80% glycerol, and observed under a fluorescence microscope. In Frantoio and Koroneiki, the number of ovules penetrated by a pollen tube was used to estimate the level of sexual compatibility. In Kalamata, the numbers of ovules penetrated by pollen tubes were not significantly different between treatments; therefore, the number of pollen tubes in the lower style was used. All the cultivars studied were self- incompatible. Frantoio (as a host) was incompatible with Koroneiki and Barnea but partially compatible with Mission. Koroneiki (as a host) was incompatible with Barnea but partially compatible with Frantoio and Mission. Kalamata (as a host) was compatible with Barnea, incompatible with Mission and Koroneiki in 2004, but partially compatible with them in 2005. In the second method, eight microsatellite markers were used for genotyping three Kalamata mother trees, 40 embryos per mother tree, and all the potential pollen donors. Genotyping data were analysed using FaMoz software, and the number of embryos assigned to each putative pollen donor was determined. Paternity analysis showed that Kalamata (as a host) was self-incompatible, compatible with Barnea, Benito, and Katsourela, but incompatible with Arbequina, Azapa, and Picual. To study the gene expression in olive pistils during flowering, a genomic approach was initiated using cDNA subtractive array analysis. Total RNA was isolated from olive pistils at two developmental stages, where self-incompatibility (SI) genes are expected to be differentially expressed: 1) small green flower buds (expression of SI genes not expected) and 2) large white flower buds containing receptive pistils just prior to opening (expression of SI genes expected). From each stage, cDNA libraries were prepared and put through forward and reverse subtractive hybridisations to enrich for differentially expressed cDNAs in stage 2. Macroarrays were prepared by printing 2304 differentially expressed cDNAs onto nylon membranes and hybridised with forwardand reverse-subtracted probes. The analysis identified 90 up-regulated cDNA clones highly expressed in receptive pistils. Further subtracted and unsubtracted hybridisations confirmed up-regulation of the majority of these cDNAs. Gene expression profiles across different tissues showed that most of the genes were pistil-specific. The expression pattern of the genes showed high similarity in Kalamata, Frantoio, Barnea, and Pendolino. All the screened genes were sequenced and their similarities were searched in the NCBI database. The most redundant and interesting up-regulated clones were those similar to a receptor protein kinase-like protein. Some versions of this protein play a role in the sporophytic SI system of Brassica and the gametophytic SI system of Papaver and rye. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1325369 / Thesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2008

The olive (Olea europaea L.) is one of the most ancient fruit trees and has been cultivated for its oil in the Mediterranean area for thousands of years. Today, the consumption of olive oil and table olives is increasing both in traditional producing countries and the entire world. Most olive cultivars are self-incompatible and do not produce a commercial yield after self pollination. In this thesis, inflorescence architecture and sexual compatibility relationships of some olive cultivars, and gene expression in olive pistils during flowering were studied. To study the inflorescence architecture of olive, 45 inflorescences in each of the cultivars Manzanillo, Mission, and Frantoio were checked every morning from flower opening to petal fall. The flower position on the inflorescence had a highly significant effect on the opening day in all cultivars. Terminal flowers and the flowers located on the primary branches opened earlier than flowers located on the secondary branches. Flower position also had a highly significant effect on gender in Manzanillo and Mission. In Manzanillo, the secondary branches had fewer perfect flowers than the primary branches. In Mission, the secondary branches had no perfect flowers at all. In Manzanillo, perfect flowers had significantly longer petal persistence than staminate flowers. To study flower competition within the inflorescence, the distal halves, on which the flowers tend to be perfect, of 120 inflorescences in three trees of Manzanillo were removed about one month before full bloom. This resulted in a highly significant increase in the percentage of perfect flowers on the proximal halves. The effects of shoot orientation and inflorescence location on inflorescence characteristics in the cultivars Frantoio, Kalamata, and Koroneiki were also studied. For each cultivar, inflorescence characteristics in three sections of shoots (top, middle, and base) and four sides of the three selected trees (north, south, east, and west) were recorded. The statistical analysis showed that basal inflorescences were shorter and with fewer flowers but with the same percentage of perfect flowers. Shoot orientation did not have any influence on these characteristics in any of the cultivars. Sexual compatibility was assessed using two methods. In the first method, controlled crossings were performed in the cultivars Frantoio, Koroneiki, and Kalamata. The pistils were harvested one week after hand pollination and stained with 0.1% aniline blue. The styles and ovules were separated, mounted in 80% glycerol, and observed under a fluorescence microscope. In Frantoio and Koroneiki, the number of ovules penetrated by a pollen tube was used to estimate the level of sexual compatibility. In Kalamata, the numbers of ovules penetrated by pollen tubes were not significantly different between treatments; therefore, the number of pollen tubes in the lower style was used. All the cultivars studied were self- incompatible. Frantoio (as a host) was incompatible with Koroneiki and Barnea but partially compatible with Mission. Koroneiki (as a host) was incompatible with Barnea but partially compatible with Frantoio and Mission. Kalamata (as a host) was compatible with Barnea, incompatible with Mission and Koroneiki in 2004, but partially compatible with them in 2005. In the second method, eight microsatellite markers were used for genotyping three Kalamata mother trees, 40 embryos per mother tree, and all the potential pollen donors. Genotyping data were analysed using FaMoz software, and the number of embryos assigned to each putative pollen donor was determined. Paternity analysis showed that Kalamata (as a host) was self-incompatible, compatible with Barnea, Benito, and Katsourela, but incompatible with Arbequina, Azapa, and Picual. To study the gene expression in olive pistils during flowering, a genomic approach was initiated using cDNA subtractive array analysis. Total RNA was isolated from olive pistils at two developmental stages, where self-incompatibility (SI) genes are expected to be differentially expressed: 1) small green flower buds (expression of SI genes not expected) and 2) large white flower buds containing receptive pistils just prior to opening (expression of SI genes expected). From each stage, cDNA libraries were prepared and put through forward and reverse subtractive hybridisations to enrich for differentially expressed cDNAs in stage 2. Macroarrays were prepared by printing 2304 differentially expressed cDNAs onto nylon membranes and hybridised with forwardand reverse-subtracted probes. The analysis identified 90 up-regulated cDNA clones highly expressed in receptive pistils. Further subtracted and unsubtracted hybridisations confirmed up-regulation of the majority of these cDNAs. Gene expression profiles across different tissues showed that most of the genes were pistil-specific. The expression pattern of the genes showed high similarity in Kalamata, Frantoio, Barnea, and Pendolino. All the screened genes were sequenced and their similarities were searched in the NCBI database. The most redundant and interesting up-regulated clones were those similar to a receptor protein kinase-like protein. Some versions of this protein play a role in the sporophytic SI system of Brassica and the gametophytic SI system of Papaver and rye. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1325369 / Thesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2008

Thesis (Msc Food Sc (Food Science))--University of Stellenbosch, 2005. / Near infrared (NIR) spectroscopy prediction models were developed for the rapid determination of the peroxide value, free fatty acid content, specific extinction coefficients at 232 and 270 nm, thiobarbituric acid reactive substances value, pigment, total polyphenol, oleic acid, linoleic acid and saturated fatty acid contents ...

Submitted by JOSIANE SANTOS DE OLIVEIRA (josianeso) on 2018-12-21T13:54:34Z No. of bitstreams: 1 Priscilla Taroco Ocanha_.pdf: 4450525 bytes, checksum: 5e9351f219cb0cb895827f96ccfaf6ff (MD5) / Made available in DSpace on 2018-12-21T13:54:34Z (GMT). No. of bitstreams: 1 Priscilla Taroco Ocanha_.pdf: 4450525 bytes, checksum: 5e9351f219cb0cb895827f96ccfaf6ff (MD5) Previous issue date: 2018-09-28 / UNISINOS - Universidade do Vale do Rio dos Sinos / A crescente demanda de produtos derivados da oliva tem despertado o interesse das indústrias que vêem neste segmento a possibilidade de aprimoramento dos aspectos nutricionais e sensoriais para melhor atendimento das demandas existentes no mercado. O objetivo deste trabalho foi desenvolver um azeite de oliva aromatizado a partir do tomilho (Thymus vulgaris L., Lamiaceae) liofilizado, que atenda aos requisitos sensoriais desejados por pessoas críticas que buscam maiores informações por aquilo que consomem, que desejam diversificar os produtos existentes no mercado e prezem por manter uma alimentação saudável (sem a presença de ingredientes artificiais na formulação) e saborosa. O desenvolvimento ocorreu a partir de formulações encontradas em literatura, aplicadas em escala piloto. O produto foi analisado físico-quimicamente e também através de um perfil cromatográfico, que possibilitaram a comprovação da manutenção da qualidade do azeites, mesmo após a aromatização, todas as análises de controle de qualidade aplicadas mostraram que os parâmetros dos azeites se mantem nos níveis exigidos pelo Ministério da Agricultura, Pecuária e Abastecimento (MAPA) (BRASIL, 2012), para azeites de oliva extra virgem. Nenhuma diferença significativa foi demonstrada entre os azeites aromatizados, em temperatura ambiente e aquecido a 60oC, desta forma, para o tomilho liofilizado, não mostrou-se eficiente aquecer o azeite para obter melhorias na extração de compostos da erva para azeite, nem para características físico-químicas no geral, assim como, não notou-se nenhum prejuízo para o azeite o aquecimento nesta temperatura, no tempo de sete dias. / The growing request for olive products are now attracting interest from industries that see in this segment the possibility of improving nutritional and sensorial aspects to better meet the market’s demands. The aim of this work was to develop an olive oil flavored with lyophilized thyme (Thymus vulgaris L., Lamiaceae), which meets the sensory requirements desired by critical consumers who seek for more information about what they consume, that wish to diversify the existing products in the market and who are most attached to maintaining a healthy and tasty diet (without the presence of artificial ingredients in the formulation). The development took place from formulations found in literature, applied in pilot scale. The product was analyzed physically chemically and also through a chromatographic profile, which allowed the confirmation of the oils high quality maintenance. Even after the aromatization, all applied quality control analyzes showed that the parameters required by the Ministério da Agricultura, Pecuária e Abastecimento (MAPA) (BRAZIL, 2012) are maintained at the extra virgin olive oils levels. No significant difference was demonstrated between flavored oils at room temperature and heated to 60°C. Therefore, for lyophilized thyme heat the oil has not been efficient either to obtain improvements in the extraction of herb’s compounds for olive oil, or for physical-chemicals characteristics in general, as well as, it was not noticed any damage in heating the oil at this temperature.

En Tunisie, l’extraction d’huile d’olives génère d’énormes quantités de sous-produits oléicoles : les grignons d’olives et les margines. De ce fait, la valorisation de ces sous-produits par deux voies (thermique et physicochimique) a été réalisée. Le premier volet concerne une nouvelle alternative qui consiste à utiliser les margines en mélange avec une matrice solide pour constituer un combustible de biomasse. La sciure et les grignons d’olives (GO) ont été choisis comme matrice d’absorption à moindre coût. Lors des essais de combustion, le bilan de carbone augmente avec l’augmentation de température, accompagné d’une augmentation du monoxyde de carbone et des composés organiques volatils. Toutefois, il a été mis en évidence la nécessité de travailler avec un temps de séjour plus élevé pour minimiser l’émission des imbrûlés. Ce procédé d’imprégnation est une technique prometteuse de réduction des impacts environnementaux de margines. Le mélange GO/margines ayant un taux d’imprégnation ≤ à 28% a montré un comportement similaire à ceux des grignons d’olives sous inerte et sous air. L’utilisation de ce mélange GO/margines semble être une solution pour la Tunisie puisque la ressource est présente et permettra d’augmenter les rendements des exploitations. Quant à la valorisation physicochimique des grignons d’olives, elle a été dédiée à l’étude de l’adsorption du nickel et du cuivre. L’étude de piégeage de ces métaux dans les cendres a révélé que le nickel et le cuivre se volatilisent peu ou ne se volatilisent pas. Ces métaux sont très faiblement solubles par lixiviation des cendres. En conséquence, le transfert vers l’atmosphère et l’environnement est alors inexistant ou minimal. / In Tunisia, oil extraction industry generates high amount of two by-products: an aqueous effluent (Olive Mill WasteWater) and a solid residue (Olive Mill Solid Waste). Two recovery pathways (thermal and physicochemical) were performed to reduce these wastes. Firstly, a combined process which includes impregnation on low-cost renewable absorbents and an energetic recovery via combustion was studied. Olive Mill Solid Waste (OMSW) and sawdust were chosen as absorbent of OMWW. During combustion tests, carbon balance increases with temperature increasing followed by an increase of CO and VOC emissions. Nevertheless, it was necessary to increase the residence time of gas in order to reduce the unburned compounds in the exhaust. This process may be a promising issue to reduce OMWW environmental impact. The mixture of OMSW/ OMWW with mass ratio of OMWW close to 28%wt shows a similar behaviour to original OMSW under inert and oxidative atmospheres. It seems that the use of this mixture of OMSW/OMWW may be a solution for Tunisia since the resource is abundant and may increase farm yields. The physico-chemical way of OMSW recovery focused on the removal of nickel and copper from wastewaters. The trapping of these metals in the ash reveals that nickel and copper are low volatilized. Moreover, these metals are not soluble during leaching. Consequently, their transfer to the atmosphere and the environment is minimal.

Thesis (Ph. D.)--University of Oregon, 2006. / Typescript. Includes vita and abstract. Includes bibliographical references (leaves 280-290). Also available for download via the World Wide Web; free to University of Oregon users.

Orientador: Robert Wayne Andrew Slenes / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Filosofia e Ciencias Humanas / Made available in DSpace on 2018-08-15T02:15:43Z (GMT). No. of bitstreams: 1 Gomes_RaquelGryszczenkoAlves_M.pdf: 1752359 bytes, checksum: 3e33e35f8962c9d619d450b209d8717e (MD5) Previous issue date: 2010 / Resumo: Olive Emilie Albertina Schreiner (1855-1920), literata sul-africana de origem anglófona, é hoje lembrada essencialmente por sua contribuição para o campo dos estudos de gênero e sexualidade, bem como por seu romance de estréia - The Story of an African Farm, publicado em 1883. Centramos nossa análise no período de expansão econômico-territorial sul-africana - aqui delimitado entre os anos 1880 e 1902 - para apreender o diálogo da escrita de Schreiner com os impactos da política imperialista britânica nas relações entre ingleses e bôeres; ingleses e nativos e nativos e bôeres. É também neste período que a literata começa a articular sua idéia de nação sul-africana e assume uma política de combate à exploração do nativo pelo sistema capitalista, além de estruturar um discurso de apoio ao bôer. Para tanto, à leitura de The Story of an African Farm associamos também o estudo de obras que receberam até então pouco destaque: Trooper Peter Halket of Mashonaland (1897) e Thoughts on South Africa (1923*). / Abstract: Olive Emilie Albertina Schreiner (1855-1920), South African writer of anglophone origin, is nowadays remembered primarily for her contribution to the field of gender and sexuality studies, as well as for her debut novel, The Story of an African Farm (1883). Focusing our analysis on the economic expansion of South African territory - period delimited here between years 1880-1902 - we intend to explore the dialog of her writings with the impacts of British imperialist policy in the relations between British and the Boers, British and natives and between natives and Boers. It is also during this period that the literate begins to articulate her idea of a South African nation and engages herself in a policy to combat the exploitation of the native by the capitalist system, besides articulating a speech in support of the Boer cause. Therefore, to the reading of The Story of an African Farm we also associated the study of some works that received little attention so far: Trooper Peter Halket of Mashonaland (1897) and Thoughts on South Africa (1923*). / Mestrado / Historia Social / Mestre em História

M.A. (English) / The fallen woman is the central figure in much of the fiction written in Britain during the nineteenth century, and she frequently reappears in colonial writing. In this study, fictional interpretations of the English victorian stereotype of the fallen woman in The story of an African Farm and From Man to Man by Olive Schreiner and in The Beadle by Pauline smith are examined. The first chapter of this dissertation is an attempt to establish the fallen woman's classic stereotypical qualities as exhibited in five British novels. Location in their historical context explains the subtle changes in metropolitan attitudes towards women in general, and fallen women in particular, brought about by the advent of organisations designed for the protection of young girls and the assertion of women's rights. Analysis reveals certain clearly defined conventional trends in characterisation, plot and imagery. The novels studied are Ruth (1853) by Mrs.Gaskell, Adam Bede (1859) by George Eliot, The Ordeal of Richard Feverel (1859) by George Meredith, East Lynne (1861) by Mrs. Henry Wood, and Tess of the D'Urbervilles (1891) by Thoma~ Hardy. In chapter two, the close interrelation between the predominant Western conceptions of manhood, colonialism, and racism is examined and an explanation given for the exaggeration in the colonies of the dual role of woman - as chaste angel or fallen devil. Examples of the social engineering undertaken in tribal or chieftainly patriarchies in Southern Africa and by the British Imperial administration at the turn of the century are given. The strong link between the oppression of blacks and women is illustrated. Dutch hierarchical notions of social caste and attitudes to women, which were in place when the British arrived, are discussed. The rapid internalisation of white male attitudes towards women of other races as reflected in the writing of white women in South Africa is then shown. Texts examined are The Letters of Lady Anne Barnard to Henry Dundas (1793 - 1803), edited by A.M. Lewin Robinson, Lady Duff Gordon's Letters from The Cape edited by John Purves, Sophie Levisseur: Memories, edited by Karel Schoeman, AVictorian Lady at the Cape (1849 -1851) edited by Alan F. Hattersley, Charlotte Moor's Marina de la Rey (i903), W.P.Livingstone's Christina Forsyth of Fingoland, the Story of the Loneliest Woman in Africa (circa 1911), Gertrude Page's The Pathway, and A Sketch of Women's Work (1893) edited by Lady Loch. It becomes evident in Saul Solomon's collection of letters entitled The contagious Diseases Act : Its operation at the Cape of Good Hope (1897), in G. Emily Conybeare's treatise entitled Womenly Women and Social Purity (1892) and in the Reverend C. Spoetstra's open letter to the editor of the Volksstem, published as a booklet under the title Delicate Matters (1896), that feminists, Members of the Cape Legislature, and clergymen were opposed to the double standard against women enshrined in Cape statutes. The reasons for their opposition are discussed. The character of the Afrikaner patriarchy which predominated at the turn of the century and into the early years of the twentieth century is described, and reasons suggested for the more mystical and sacrificial approach of this group towards the fallen woman as detected in the Reverend Spoetstra's letter. Having outlined the distortion of British attitudes towards women in South Africa in chapter two, I then examine the fictional interpretations of the fallen woman in Olive Schreiner's novels The story of an African Farm and From Man to Man. Her treatment of this character is related to the British stereotypes and to the influence of colonial attitudes to women and race already established. Biographical details and facts related to the composition of From Man to Man, which are of value in determining why the novelist chose, or was compelled, to represent her characters in the way she did, are studied. A progression of ideas regarding female autonomy and independence is traced in her novels by means of close textual analysis. The chapter ends with an evaluation of Schreiner's attitude to race and racism and of Schreiner's colonial version of a fate of women.

Une meilleure connaissance des stratégies migratoires à l’échelle des espèces, des populations et des individus devrait faciliter l’identification de facteurs limitants et des défis rencontrés par les migrateurs. Les grives (Catharus spp.) de l’est de l’Amérique du nord semblent suivre le corridor de migration de l’Atlantique pour rejoindre leur aire d’hivernage plus au sud à l’automne. Jusqu’à récemment, les technologies ne nous permettaient pas de déterminer les différences entre les espèces, les populations et les individus à un niveau de résolution plus élevé. Cette étude compare les stratégies migratoires automnales individuelles de la Grive à dos olive, de la Grive de Bicknell et de la Grive à joues grises grâce à un réseau coordonné de radio-télémétrie, dont les récepteurs sont déployés dans l’est du Canada et des États-Unis. Les routes migratoires, la vitesse de migration et la variabilité interindividuelle ont été analysées. La Grive à dos olive migrait plus à l’ouest et utilisait davantage l’axe du Saint-Laurent et des Grands Lacs que la Grive de Bicknell et la Grive à joues grises, ces dernières longeant davantage la côte Atlantique. Les haltes migratoires utilisées par les trois espèces semblaient plus fréquentes au sud-est des États-Unis que plus au nord. Cependant, la progression vers le sud de la Grive à joues grises était plus rapide que celle des deux autres espèces. Les trois espèces avaient une variabilité individuelle importante (> 10 degrés) dans leur longitude maximale de migration. Dans une perspective de conservation, ces différences pourraient se traduire en enjeux différents entre les espèces, les populations et les régions. Les résultats de cette étude illustrent d’une nouvelle manière la polyvalence des stratégies migratoires chez des espèces aux caractéristiques écologiques similaires, et gagneront à être intégrés dans des efforts de synthèse visant une connaissance plus profonde des enjeux liés à la migration des oiseaux. / A better knowledge of the migratory strategies at species, population and individual levels should facilitate the identification of limiting factors and challenges faced by migrants. Catharus thrushes from eastern North America appear to follow the Atlantic migration corridor to reach their wintering grounds further south in the fall. Until recently, technologies did not allow us to determine the differences between species, populations and individuals at a greater resolution. This study compares the individual fall migration strategies of the Swainson’s Thrush, the Bicknell's Thrush and the Gray-cheeked Thrush using a coordinated radio-telemetry network, which receiving stations are deployed in eastern Canada and the United States. Migration routes, migration paces, and individual variability were analyzed. The Swainson's Thrush migrated further west along the St. Lawrence River and the Great Lakes than Bicknell's Thrush and Gray-cheeked Thrush, which migrated along the Atlantic coast. Stopovers by the three species appeared more frequent on the southeastern United States than further North. However, Gray-cheeked Thrushes migrated faster than the other two species. Conspecifics exhibited significant individual variability (> 10 degrees) in maximum migration longitude. From a conservation perspective, these differences could translate into different stakes depending on species, populations and regions. The results of this study illustrate in a new way the versatility of migration strategies within species with similar ecological characteristic, and will benefit from being integrated into synthesis efforts aiming a deeper knowledge of the issues related to bird migration.

The research within this thesis sought to determine the ability of various animal derived fats and plant derived oils to support the survival of several pathogenic cocktails over a multitude of storage times. The Salmonella study explored the survival rate of a four strain Salmonella cocktail in beef tallow, pig lard, duck fat, coconut oil, and extra virgin olive oil over seven days at 26˚C and 37˚C storage. The animal fats and the coconut oil supported the survival of the bacteria until the conclusion of the study. The Shiga-toxin producing Escherichia coli study explored the survival rate of a five strain STECs cocktail in extra virgin olive oil over seven days at 26˚C and 37˚C storage. The two Listeria studies explored the survival rate of a four strain Listeria monocytogenes cocktail in extra virgin olive oil over several time periods with different frequencies of sample mixing. In vitro, all genuses showed a 2.5-log cfu/mL to ≥ 7-log cfu/mL reduction in the extra virgin olive oil by the conclusion of the experiments. Extra virgin olive oil was then applied to cooked pork tenderloin, cheddar cheese snack squares, and turkey lunchmeat in hopes of inhibiting the L. monocytogenes cocktail. No reduction was observed.