Gopherus polyphemus, commonly known as the Gopher Tortoise, is a dryland reptile native to the southeastern United States. It is commonly a resident of longleaf pine and dry oak sand hill habitats. It is considered a keystone species because they dig deep burrows that provide shelter to them as well as many other animals. Habitat loss, fragmentation, and disease are major threats and have caused this species to be federally listed as a threatened species under the Endangered Species Act (ESA). Disease is a major threat to the gopher tortoise’s survival, and with declining populations, the need to investigate pathogens is crucial. Herpesvirus, is known to contribute to upper respiratory tract diseases (URTD) in G. polyphemus and is the primary focus of this project. Due to high mutation rates in the virus, a modified version of PCR, nested PCR, was conducted on eye and nose swabs and blood samples obtained from G. polyphemus to detect the presence of the alpha herpesvirus pathogen. The positive samples were then sent for genetic sequencing to confirm the occurrence of the pathogen. The detectability of Herpesvirus in eye and nose swabs was compared to blood and lymph samples and statistical tests concluded that both sample types had the same detectability.

The national mammal of the United States, the American Bison (Bison bison) was once nearly extinct. Populations have recovered to the degree that thousands roam the Great Plains today. Due to their large numbers and body size, this species has an oversized impact on the ecological communities where it lives and is considered a keystone herbivore in modern North American grasslands. This study explores the detailed, seasonally resolved, paleoecology of seven bison from the Greater Yellowstone Ecosystem during the Late Holocene through stable isotope analyses and species niche modeling. Isotopic analyses of δ13C, δ15N, and δ18O reveal that bison within high elevations regularly foraged on C3 vegetation while traveling among the valleys and ridges of ecoregions similar to those of modern-day. Species distribution models provide a bimodal niche, with best-suited temperatures of 4-8°C and 16-26°C, and topographic ranges of 250-800m and 2,000-4000m.

<p>Social learning, defined as learning from other individuals, has been well studied in vertebrates and social insect species. In order to promote further understanding of the evolution of social learning, I tested a non-social insect for social learning and socially influenced behaviour. The desert locust (Schistocerca gregaria) is a gregarious insect which has the opportunity for social learning and can benefit from such ability. Locusts showed rapid individual learning, preferring a diet they have experienced over another of equal nutritional quality. Adult locusts also showed socially influenced behaviour, preferring to eat and lay eggs in the vicinity of other locusts over doing so alone. Fifth instar locusts did not show the same socially influenced behaviours. Neither adult nor nymphs showed social learning after interacting with previously fed models or after observing models feed through a screen. These results provide evidence for socially influenced behaviour in locusts and for a difference in social behaviour between nymphs and adults. Further research utilizing locusts as a model system may help us gain a better understanding of the evolution of social learning.</p> / Master of Science (MS)

Salt marshes are important habitats that provide many ecosystem services, but they are susceptible to the impacts of sea level rise (SLR), often resulting in emergent vegetation loss. In areas with enough sediment input, marshes can keep pace with SLR by gaining elevation or through upland migration. However, salt marshes in areas with limited sediment input, such as the Grand Bay National Estuarine Research Reserve, often cannot keep pace with sea level rise. Additionally, the rate of SLR is increasing making it more difficult for marshes to keep pace. To assess the short-term response of marsh vegetation to sea level rise, percent cover, stem density, and elevation, data from 2016 to 2020 in four different marsh elevation zones were analyzed in this study. Results demonstrated that the four marsh elevation zones are responding disparately to SLR. These findings indicate that it is imperative to implement restoration plans to account for site variability to conserve these vital habitats.

The establishment of monoculture plantations of exotic tree species is common practice for supplementing native timber stocks. Such plantations typically provide inferior habitat for wildlife compared to native forest, which may result in a net reduction in biodiversity. However, some studies report that plantations may increase net biodiversity at the landscape scale by introducing novel habitats or supplementing existing natural forests. Using point count surveys, I examined six mature Norway spruce (Picea abies) plantations in western Massachusetts in 2016 and 2017 to evaluate bird use of these habitats relative to native forest stands. Count data were analyzed using N-mixture models to correct for imperfect detection, providing more accurate estimates of true abundance. Our findings showed that overall species richness for spruce plantations was not significantly lower than that of native forest habitats. Red-breasted nuthatch (Sitta canadensis) and golden-crowned kinglet (Regulus satrapa) were most abundant in spruce plantations. Conifer dependent species such as Blackburnian warbler (Setophaga fusca) and brown creeper (Certhia americana), were significantly more abundant in spruce plantations relative to native deciduous, hemlock, and mixed stands. Species that heavily associate with broadleaf habitat were rarely observed in spruce plantations. Species that associate with eastern hemlock habitat, such as Blue-headed vireo (Vireo solitarious) and black-throated green warbler (Setophaga virens) were observed using spruce plantations at similar levels as eastern hemlock stands. These results demonstrate that Norway spruce plantations can provide suitable habitat for native species associated with conifers, which is significant given projected continued decline of eastern hemlock in response to the hemlock wooly adelgid (Adelges tsugae). Although large-scale conversion of native forest to plantations would likely lead to a loss in biodiversity, land managers could be justified in allowing small-scale plantations to persist without suffering negative impacts to native biodiversity.

Artificial lighting at night is a growing worldwide phenomenon that can negatively impact flora and fauna in the surrounding area. With new technology, there is now opportunity for change: newer LEDs can emit specific wavelengths of light that may be less ecologically damaging. In this study, we aim to describe the circadian spectral sensitivity of two phylogenetically distant urban spider species with similar life histories, the Araneoid Parasteatoda tepidariorum and non-Araneoid Pholcus manueli, to determine differences between them. We exposed these spiders to progressively dimmer levels of white, blue, green, or red light. Despite evidence that spiders lack visual sensitivity to red light, we found that not only could both species entrain to the dimmest red light, P. tepidariorum had stronger entrainment to green and red light than white light. This study suggests that wavelength selection for ALAN will require a nuanced approach considering the sensitivities of individual local species.

Population structure is influenced by extrinsic factors, such as landscape architecture and dispersal barriers. Lotic network architecture is known to constrain ecological, demographic and evolutionary processes, including population genetic structure. I assessed the population structure of a widespread aquatic salamander, Necturus maculosus, across three river basins in central and eastern Kentucky. I examined the role of network architecture, anthropogenic barriers, and spatial scale on patterns of population structure. I also provided a review of N. maculosus capture methods and offer an improved trap design. I identified significant structuring between the combined Licking/Kinniconick basin and the Kentucky River basin, with further structure within each basin. I found evidence for both hierarchically organized populations structure (e.g. Stream Hierarchy Model), as well as population structure unaffected by network hierarchy (e.g. Death Valley Model). These results highlight the importance of scale when examining population structure. Whereas one model may suffice to explain population structure at a local scale, a second model may be necessary to accurately describe the population structure across larger spatial scales. These results suggest that local factors affect population structure uniquely across a species’ range, and support a multi-model approach for assessing population structure.

Located at the interfaces of terrestrial and marine environments, coastal habitats are inherently vulnerable to the effects of global change. Barrier island systems in particular serve not only as protective buffers against storm events, but also as sentinel ecosystems for observation of the impacts of sea level rise, and of increasing storm frequency and intensity. In the mid-Atlantic region, shrub thickets of Morella species compose the dominant forest community. The often monospecific nature of these plant community assemblages is advantageous to ecological studies and cross-scale applications; the relatively low diversity facilitates transitions between scales. My objective was to investigate the distribution and community roles of lianas in mid-Atlantic barrier island forest communities. I quantified environmental variables at two barrier habitats with differing site management histories and corresponding topography, and found that abiotic factors affected distributions of woody species, which subsequently affected vine species distributions. Some association of prevalent vine species with the common woody plants Prunus serotina and Morella cerifera was observed, though neither vines nor woody species demonstrated significant species-specific phytosociological associations. Vines demonstrated a long-lasting effect of arresting or delaying succession, and are potentially responsible for the lack of redevelopment of mature maritime forest at these sites. At Hog Island, Virginia, remotely-sensed data were utilized to determine the three-dimensional structural effects of vine infiltration in woody canopies. Vines were found to reduce canopy height and depth, and increase density, short-term diversity, and light-intercepting biomass. Significant vine infiltration can accelerate senescence of shrub thickets, but often results in persistent tangled masses of vegetation which reduce recruitment of later-successional species. These effects may represent long-term, lasting impacts of vine establishment and expansion in these habitats, affecting community succession towards diverse and stable maritime forest, and significantly altering resource dynamics in these sensitive ecosystems.

Dune grasses are integral to biogeomorphic feedbacks that create and alter foredunes and barrier island stability. In a glasshouse study, Ammophila breviligulata Fern. and Uniola paniculata L. were planted together and subjected to sand burial to quantify morphological and physiological response. Ammophila breviligulata physiological and morphological performance declined when planted with U. paniculata but U. paniculata was not affected when planted with A. breviligulata. Burial had a positive effect on A. breviligulata and U. paniculata as indicated by electron transport rate and total biomass at the end of the experiment. Due to their different growth strategies, A. breviligulata and U. paniculata form continuous versus hummocky dunes, respectively. As global temperatures rise and U. paniculata migrates into A. breviligulata dominated habitat, A. breviligulata performance may diminish, and changes in dune form could result in altered island stability via increased overwash. Foredune community structure could also change due to the shift in dominant species which could alter dune succession.

Stability of coastal systems are threatened by oceanic and atmospheric drivers of climate change. Sea-level rise compounded with increased frequency and intensity of storms emphasizes need for protection of inner island systems by dune formations. Dune building processes are affected by interactions between growth of ecosystem engineering dune grasses and environmental factors associated with disturbance such as sand burial and salt spray. Climate change may also cause latitudinal expansion of some species, resulting in emergence of competitive interactions that were previously absent. Topographic structure of coastlines, traditionally influenced by sand burial, could change as a result of competition emergence. My goal was to determine if species functional trait responses to common abiotic factors are altered by novel and current biotic interactions. I performed a multi-factorial greenhouse experiment by planting three common dune grasses (Ammophila breviligulata, Uniola paniculata, and Spartina patens) in different biotic combinations, using sand burial and salt spray as abiotic stressors. I hypothesized that biotic interactions will cause these dune grasses to shift functional trait responses to abiotic factors that are associated with dune building. I found that plants consistently decreased in biomass when buried. I also found that competition between A. breviligulata and U. paniculata negatively affected dune building function traits of A. breviligulata. This indicates that competition with U. paniculata could alter dune structure. In comparison A. breviligulata had a positive interaction with S. patens, which increased functional trait responses to abiotic stress. Last, we found that competitive intransitivity could occur between these species. My results can be used to make predictions on cross-scale consequences of novel competitive events. This experiment also provides evidence that consideration of local biotic interactions is important in understanding connections between plant level dynamics and large-scale landscape patterns in high stress environment.