MENVSC / Department of Ecology and Resource Management / Certain communities in Sekhukhune area are known to be practicing geophagia. Existing standard questionnaires as adopted to generate data on human geophagia included aspects on demography, socio-economics, cultural, ecological, physico-chemical aspects, indigenous knowledge and health effects of geophagic consumers. These data were gathered through distribution of questionnaires to 200 geophagic participants, of which 135 were from Ga-Nchabeleng Village and 65 from Mphanama Village. Both the Villages were based in the rural settlements in Sekhukhune area. In this study the behaviour of geophagic individuals was investigated and cytotoxicity of plant remains consumed in geophagic soils was evaluated for any toxicity. A total of 17 different geophagic sites were identified. Six geophagic sites each were from Ga-Nchabeleng Village and six from Mphanama Village. Five other sites were selected from sites where geophagia was not practiced and were recorded as the control sites. All the 17 soil samples were analysed using Munsell Soil Color charts for soil colour classification. Samples of plant remains were collected from the same geophagic sites where soil samples were collected. Due to similar plants and vegetation type from 17 different geophagic sites, a composite study was adopted. Geophagic consumers in Ga-Nchabeleng Village identified four plant species of plant remains consumed in the soil in the area and Mphanama village identified five species different from Ga-Nchabeleng plants. Samples of plant remains were grouped according to comparable features or characteristics. Sample 1 of plant remains was composed mainly of grasses; Cynodon dactylon, Aristida congesta and Eragrostis rigidior whereas sample 2 of plant remains was composed of Acacia plant; Vachellia tortilis. The two samples of plant remains were collected from Ga-Nchabeleng Village. Sample 3 of plant remains was composed of creeping, perennial weed herbs; Alternanthera pungens Kunth and Alternanthera lorentzii. Sample 4 of plant remains was composed of prominent woody plants; Combretum apiculutum, Kirkia wilmsii and Boscia albitrunca. Samples of plant remains 3 and 4 were collected from Mphanama Village study sites. Sample 5 of plant remains was the control site and composed mainly of Acacia plants; Vachellia nilotica, Acacia vii mearnsii and Vachellia tortilis and were collected from sites not used for geophagic practices. The plant remains parts mostly consumed were roots (50%) in Ga-Nchabeleng, whereas in Mphanama Village they were stems (54.5%) and the control site had leaves at 62.5%. The five samples of plant remains were recovered using physical separation method. The plant remains were washed and dried. Retch Muhle grinding machine was used to ground the samples. Methanol was used in the extraction of all the samples of plant remains. The result from the administration of the questionnaire revealed that geophagia in this area was practiced by both male and female Sepedi-speaking individuals. Ga-Nchabeleng Village had more female geophagic participants, whereas Mphanama Village had more males who have almost undergone secondary school. However, in general for the study there were more female geophagic consumers. Geophagic consumers ingest soil known locally as Mobu, Letsopa or Leraga collected mostly from the riverbanks, mountains/hills and valleys with only a few that indicated termite mounds. Geophagic consumers in the study used colour, among other things, to describe their soil of preference. The study consisted of 200 participants of whom 172 represented the geophagic group and 28 were the control group, aged between 18-65 years analysed using chi-square crosstabulation. There was no significant difference in human health effects associated with geophagia between the geophagic group and the control group. There was also no association established between soil consumption and other non-food substances between geophagic group and control group. Chi-square (χ2) analyses revealed a significant association of gender with geophagic habits (p<0.05), while there was no association of age, educational level, income source and marital status (p>0.05) with geophagic habits. Findings of the survey when two villages are combined revealed that more females (75.60%) practice geophagia compared to males (24.40%). The respondents from both study sites preferred digging technique when collecting the soil. It was established that craving was mainly the reason behind the practice in the study area. Hygiene and environmental conditions were not considered when mining viii geophagic soil as the majority of them used dirty utensils, hands for collection and non-sterile bags and tins for packaging. Some of the soils were collected close to waste dumping sites as seen whilst visiting geophagic mining sites. Majority of the consumers had little or no knowledge that the soil could be harmful or if it contained any contaminants. This sample of interviewees provided valuable information on human geophagic practices in Sekhukhune area. It became clear that this practice was entrenched in the cultural behaviour of people in the area and a need for educating them on health related aspects. The cytotoxicity of methanolic extracts of plant remains on HEK-293T cell line was evaluated using MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay for cell viability. The 50% cytotoxic concentration (CC50) was defined as the compound’s concentration (500, 250, 125, 62.5, 31.25, 15.625 and 7.8125 μg/ml) required for the reduction of cell viability by 50%. Evaluation of cell viability showed the methanolic extracts of plant remains on HEK-293T cell line ranged in the order of plant remains extract=3>1>4>2>5 according to their cytotoxicity activities. Plant remains extract 3 from Mphanama Village showed high cytotoxicity with a CC50 of 13.75 μg/ml, followed by plant remains extract 1 at 16.68 μg/ml, plant remains extract 4 at 58.95 μg/ml, plant remains extract 2 at 92.75 μg/ml and the control at 251.4 μg/ml, respectively. In the study only the methanolic extract was investigated for cytotoxicity using HEK-293T cell line. Further research need to be conducted on the individual plants of each plant remains to be able to have conclusive results on the cytotoxicity profile. This will indicate which specific plant part is toxic or whether they exhibit a higher CC50 only when in combination.

MESHWR / Department of Hydrology and Water Resources / Communities in South Africa have been noted to consume earthy materials such a soil among others. Geophagy is largely practised in the rural areas as opposed to urban places, and in these places the level of sanitation is low and people usual practice open defecation. The practice of Geophagia has been associated with cultural, medicinal, psychological, religious and nutritional deficiency among others. The aim of the study was to investigate the effects of open defecation on geophagic soils and water resources in Siloam village. The study also aimed to understand the reasons why people practice geophagia in Siloam village and the health effect associated with consumption of geophagic soils. To carry out the aim of the study, a questionnaire survey was conducted among women who practice geophagia between the ages of 18 and above in Siloam village. Two hundred and eighty-three (283) women were selected using snowball sampling method to take part in the study. From the (283) women, (200) women represent the geophagic group and (83) women represent the control group. The existing standard questionnaire adopted to generate data on human geophagia included aspects on demography, socio-economic, cultural, ecological, physicochemical aspects, indigenous knowledge and health effects of geophagic consumers. Participants were asked basic questions on why they practice geophagy, their geophagic material preferences, where they collect the geophagic material and other related questions. A total of twelve soil samples were collected from Siloam village, from the twelve soil samples collected eight were collected from sites known for geophagic practice and another four soil samples were collected from sites where geophagia is not practiced and the samples were used as control soil samples. All the twelve soil samples were analysed for the presence of geohelminths ova using Ammonium Bicarbonate Protocol (AMBIC protocol). The AMBIC protocol consists of four analytical procedures, namely, sample preparation, sample washing with AMBIC solution, geohelminths ova recovery through a modified zinc flotation method and microscopic analysis. vi Furthermore, a total of twelve water samples were collected from Nzhelele River and ponds water in Siloam village. From the twelve water samples, eight samples were collected close to where geophagic materials were collected and where open defecation is reported to be taking place and the remaining four water samples were collected randomly from Nzhelele river and water ponds in Siloam village and was recorded as a control group. A total of 12 water samples were analysed for total coliform and faecal coliform Escherichia Coli (E.coli) indicator using the membrane filter technique. The results from the administration of the questionnaire revealed there was prevalence in the practice of geophagia in the area. The study found that most of the villagers in Siloam consumed soil nearly on daily basis. According to the results, only women were involved in the geophagic practices in the area. Additionally, the study revealed that geophagic consumers were mainly in the age of child bearing age group and reddish and yellowish soils were the most preferred. Furthermore, the results showed that geophagic consumers in the area generally consumed soil commonly because of cravings and pregnancy, however, those who were not pregnant also consumed soil. The respondents reported that they mostly consumed clay and this material was consumed in its dry state and mostly unprocessed, if processed, it would be baked. This geophagic material was mainly found in the wild (riverbed, valley etc.); it was also found that most of the soil consumers did not know that the substances they consumed could be harmful to them. Among those who knew the consequences of consuming the material stated that soil consumption causes constipation, tooth decay, body poisoning, and abdominal pains. The results from the control group, 83 women who do not practice geophagia, aged between 18 and above, analysed using chi-square revealed a significant association of age with consuming soils (p<0.05), while there was no association of income source (p>0.05) and educational level (p>0.05) with consuming soils. Chi-square (χ2) analyses further revealed that there was no association of knowledge on the harmful nature of the substance (p>0.05), frequency of getting infections (p>0.05) and experiencing chronic illnesses (p>0.05) with frequency of consuming soils. vii The results of geophagic soils revealed the absence of geohelminths ova in the entire geophagic sample. Meaning geophagic consumers in Siloam village are not at risk of acquiring geohelminths infection which may be of potential risk to human health. However, geophagic consumers may be exposed to various other potentially hazardous biological and non-biological soil contents. The results of the water samples revealed that most of the water samples in the areas where open defection is reported to be practised had higher composition of faecal and total coliform bacteria. The composition was above the South African recommended standard for negligible risk of microbial infection. This has caused a great threat to those who consume soil collected near water resources, especially from riverbed and those who also use these water sources on their daily basis. The study recommends that geophagic consumers should continue to bake their materials before consumption as it might reduce the bacteria and toxic substances found in soils. The study also recommends that the residents of Siloam be made aware of the potential health hazards that might be posed to soil consumers and the effects of practicing open defecation near community water resources as high level of faecal coliform (E. coli) were found in the water. / NRF

MESMEG / Department of Mining and Environmental Geology / Literature indicated that several mineralogical identification studies have been carried out on clays but few have focused on the characterisation of geophagic materials from South Africa. Large quantities of earth materials are consumed daily in Mashau Village, however, their mineral content and geochemical compositions had not been determined. Moreover, though the consumption of geophagic materials is very common in the village, the associated health implications had not been addressed. Thus, the main aim of the research was to mineralogically and geochemically characterise geophagic materials commonly ingested in Mashau Village and infer on possible health implications that could result from their consumption. Questionnaires were administered to geophagists in the study area with the aim of generating data on the prevalence of geophagia and the motivations for the practice. Geophagic soils and their parent rocks (for determination of provenance) were sampled and analysed for mineralogical and geochemical content. Geophagic soil samples were subjected to the following physicochemical analyses: colour, particle size distribution, pH, cation exchange capacity (CEC) and electrical conductivity (EC). An x-ray diffractometer (XRD) was used for mineralogical analysis while major oxides and trace elements abundances were determined using x-ray fluorescence (XRF) spectrometry and laser ablation inductively coupled mass spectrometry (LA-ICP-MS), respectively. Furthermore, provenance of the geophagic materials was determined using data obtained from mineralogical and geochemical analysis. Inferred health implications were based on the physico-chemical, mineralogical and geochemical data obtained. Outcomes of the questionnaire survey revealed craving to be the motivation for geophagia in Mashau Village. Although the practice seemed to be prevalent in females of certain ages, it was certainly not limited to gender, age, educational level or socio-economic status. Out of the 20 geophagic samples, 3 samples were brown, 2 had a strong brown colour and another 2 had a light olive brown colour. Other soil colours were less common, as each colour was only observed in one sample. The sand fraction dominated the samples; the clay content was low, giving the samples a sandy clay loamy texture. The pH of the soil ranged from being slightly acidic (5.4) to being slightly alkaline. The CEC values were very high ranging from 17 t0 109 meq/100 g. vii The EC values were also high (ranging from 11.2 to 245 μS/cm) indicating a high amount of soluble salts. Mineralogical analysis of geophagic soils identified quartz, microcline, plagioclase, hornblende, dolomite, muscovite, kaolinite, smectite, talc, anatase, hematite, ilmenite, chlorite and epidote with quartz and kaolinite being the dominant minerals. Actinolite, augite, chlorite, epidote, forsterite, magnetite, muscovite, plagioclase, quartz, sepiolite and microcline were the minerals identified in rock samples. Geochemical analysis for major oxides content (SiO2, TiO2, Al2O3, Fe2O3, MnO, MgO, CaO, Na2O, K2O, P2O5 and Cr2O3) indicated that both geophagic soils and parent rocks were mainly composed of silica and alumina. Trace elements geochemistry showed a depletion of LREEs and an enrichment of HREEs in geophagic soils. The results also revealed that the REEs were enriched in the bulk fraction than in the clay fraction. Relative to the Upper Continental Crust (UCC) compositions, the concentrations of trace elements in geophagic soils were generally low. Provenance determination results showed that geophagic soils in Mashau were derived from basalts and sandstones. Majority of the samples were formed as a result of intense weathering while some were as a result of intermediate weathering. The negative health implications of the studied materials could include perforation of the colon, damage of the dental enamel and anaemia. However, geophagic materials could also be a good source of mineral nutrients and beneficial for reduction of nausea during pregnancy. / NRF

The research is concerned with how the post-mortem report can be used as a source of information in the investigation of murder cases to identify crime, individual crimes and trace suspects, etc. The understanding of the post-mortem report, and the information which can be obtained from it, will assist the police investigators to handle murder cases in a professional way. The purpose of this research was to evaluate the existing manner in which police investigators use post-mortem reports in their investigations, with the intention of determining its strengths and weaknesses, and considering how the usage can be improved. Secondly, the researcher wanted to explore how investigators use postmortem reports as a source of information in their investigations. To accomplish this, the researcher perused international and national literature in an attempt to explore the field. The researcher wanted to apply the new research knowledge in order to develop good practice in the field. This has been done by recommending new procedures to enhance performance and to improve the conviction rate in court cases. / Police Practice / M.Tech. (Forensic Investigation)

The research is concerned with how the post-mortem report can be used as a source of information in the investigation of murder cases to identify crime, individual crimes and trace suspects, etc. The understanding of the post-mortem report, and the information which can be obtained from it, will assist the police investigators to handle murder cases in a professional way. The purpose of this research was to evaluate the existing manner in which police investigators use post-mortem reports in their investigations, with the intention of determining its strengths and weaknesses, and considering how the usage can be improved. Secondly, the researcher wanted to explore how investigators use postmortem reports as a source of information in their investigations. To accomplish this, the researcher perused international and national literature in an attempt to explore the field. The researcher wanted to apply the new research knowledge in order to develop good practice in the field. This has been done by recommending new procedures to enhance performance and to improve the conviction rate in court cases. / Police Practice / M.Tech. (Forensic Investigation)

MSc (Microbiology) / Department of Microbiology / See the attached abstract below