Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-10-26T16:56:10Z No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) deliane_cristina_costa.pdf: 814839 bytes, checksum: 2b161efbc9e0911afd6a3fd084af909a (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-10-26T16:57:27Z (GMT) No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) deliane_cristina_costa.pdf: 814839 bytes, checksum: 2b161efbc9e0911afd6a3fd084af909a (MD5) / Made available in DSpace on 2015-10-26T16:57:36Z (GMT). No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) deliane_cristina_costa.pdf: 814839 bytes, checksum: 2b161efbc9e0911afd6a3fd084af909a (MD5) Previous issue date: 2011-07-15 / O presente estudo teve como objetivo avaliar a larvicultura de Brycon sp. (piabanha-do-Pardo) sob distintas cores de aqu?rios e fotoper?odos, em dois experimentos distintos, com dura??o de 15 dias cada. Os trabalhos foram realizados em Machado Mineiro, distrito de ?guas Vermelhas (-15? 44'S e -41? 27'W), em dezembro de 2009 e janeiro de 2010. Em ambos os experimentos foram utilizados exemplares com 24 h, p?s-eclos?o. Durante os cinco primeiros dias de vida, as larvas foram alimentadas com 15 larvas de curimba larva-1 de piabanha dia-1 e, ap?s o sexto dia, com ra??o comercial. Os delineamentos foram inteiramente casualizados com cinco tratamentos e quatro repeti??es cada. Ao t?rmino dos trabalhos, foi determinada a sobreviv?ncia e determinada a biometria (peso e comprimento). O primeiro trabalho objetivou avaliar o desempenho e sobreviv?ncia de larvas de Brycon sp. (piabanha-do-Pardo) cultivadas em aqu?rios de cores claras e escuras. Os tratamentos consistiram na utiliza??o de aqu?rios de diferentes cores, sendo: tr?s cores claras (branco, verde e azul) e duas escuras (marrom e preto). Os exemplares foram estocados na densidade de 15 larvas L-1 em 20 aqu?rios com 4 L de volume ?til cada. O aqu?rio marrom levou a uma maior sobreviv?ncia (84,17%) e menor mortalidade (8,33%) e canibalismo (6,25%), quando comparado ao azul, que resultou em menor sobreviv?ncia (66,25%) e maiores mortalidade (15,00%) e canibalismo (21,25%). Os aqu?rios branco, verde e preto n?o diferiram entre si e do azul e marrom. Verificou-se uma diferen?a na pigmenta??o da pele das larvas (p<0,05), ficando mais escuras nos aqu?rios marrom e preto e mais claras nos ambientes branco, verde e azul. Aqu?rios marrons s?o recomendados para a larvicultura de piabanha-do-Pardo por promoverem maiores valores de sobreviv?ncia e menores taxas de mortalidade e canibalismo. O segundo experimento objetivou determinar a influ?ncia do fotoper?odo no crescimento e na sobreviv?ncia de larvas de Brycon sp. (piabanha-do-Pardo). Os exemplares foram estocados na densidade de 15 larvas L-1 em 20 aqu?rios com 5 L de volume ?til cada. Elas foram cultivadas sob diferentes fotoper?odos: 0HL (HL: horas de luz), 6HL, 12HL, 18HL e 24HL. Larvas cultivadas em 0HL, 12HL, 18HL e 24HL apresentaram maiores valores de peso, comprimento total e TCE quando comparadas ao de 6HL. Maiores valores de Fulton foram observados para fotoper?odos de 24HL, 0HL e 18HL, respectivamente, refletindo uma boa condi??o corporal das larvas nesses tratamentos. Menores valores foram registrados para 12HL e 6HL. A sobreviv?ncia reduziu com o aumento do fotoper?odo a partir de 12HL. Portanto, sugere-se a utiliza??o de um fotoper?odo intermedi?rio em torno de 9 h de luz, por promover maiores valores de sobreviv?ncia. A determina??o das cores e fotoper?odo adequados demonstrou ser importante para otimiza??o das t?cnicas de manejo. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT This study aimed to evaluate the larviculture of Brycon sp. (Piabanha-the-Pardo) under different colors of aquariums and photoperiod, in two separated experiments, lasting 15 days each. The studies were conducted in Machado Mineiro, ?guas Vermelhas District (-15? 44'S and -41? 27'W) in December 2009 and January 2010. In both experiments we used specimens 24 h after hatching. During the first five-day-old, the larvae were fed with 15 larva/day of curimba/piabanha and after the sixth day, with commercial diet with 55% crude protein twice a day. The designs were completely randomized design with five treatments and four replicates. At the end of the work there was determined the survival and performed the biometry of all animals (weight and length), the data were submitted to ANOVA and means were compared by Tukey test at 5% probability. The first study aimed to evaluate the performance and survival of larvae of Brycon sp. (piabanha-do-Pardo) grown in aquaria with light and dark colors. The treatments consisted in the use of aquariums in different colors: three colors (white, green and blue) and two dark (brown and black). The specimens were stored at a density of 15 larvae/L in 20 aquariums with total volume of 4L each. The brown aquarium led to greater survival (84.17%) and lower mortality (8.33%) and cannibalism (6.25%) compared to blue which resulted in lower survival (66.25%), mortality (15.00%) and cannibalism (21.25%). While the white, green and black aquariums did not differ when compared to the blue and brown. There was a difference in skin pigmentation of the larvae (p<0.05), becoming more dark in the brown and black aquariums and more clear in white, green and blue ambients. Brown aquariums are recommended for the larvicultura of piabanha-do-Pardo for promoting the highest survival values and lower rates of mortality and cannibalism. The second experiment aimed to determine the influence of photoperiod on growth and survival of of Brycon sp. larvae (Piabanha-the-Pardo). The specimens were stored at a density of 15 larvae/L in 20 aquariums with 5L useful volume each. They were grown under different photoperiods: 0HL (HL: hours of light), 6HL, 12HL, 18HL and 24HL. The larvae maintained at 0HL, 12HL, 18HL and 24HL showed the best results for weight, length, specific growth rate (TCE) and condition factor of Fultom (K), when in comparison to 6HL. Higher Fulton values were observed for photoperiods of 24HL, 0HL and 18HL, respectively, reflecting a good body condition of the larvae in these treatments. Lowest values were recorded for 12HL and 6HL. Survival decreased with increasing photoperiod from 12HL. Therefore, it is suggested the use of an intermediate photoperiod around 9 h 23 min of light, due to promote higher levels of survival. The determination of the colors and appropriate photoperiod has shown to be important for optimizing management techniques.

Submitted by PPG Biologia Celular e Molecular (bcm@pucrs.br) on 2018-02-26T11:20:29Z No. of bitstreams: 1 FERNANDA_ROSA_SAWITSKI_TES.pdf: 5519043 bytes, checksum: d8234f6f341fd9d5f2c4a39db88b714c (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-02-26T20:03:16Z (GMT) No. of bitstreams: 1 FERNANDA_ROSA_SAWITSKI_TES.pdf: 5519043 bytes, checksum: d8234f6f341fd9d5f2c4a39db88b714c (MD5) / Made available in DSpace on 2018-02-26T20:08:29Z (GMT). No. of bitstreams: 1 FERNANDA_ROSA_SAWITSKI_TES.pdf: 5519043 bytes, checksum: d8234f6f341fd9d5f2c4a39db88b714c (MD5) Previous issue date: 2017-11-07 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Genes related to externally visible characteristics (EVC) present a great importance for the prediction of skin and eye colors in humans. In this study, we tested the ability of a set of SNPs in pigment-related genes to predict skin and eye colors in an admixed south Brazilian population. First, we used the Cinderella - Tiana - Snow White model to analyze the allelic distribution of eight biallelic SNPs in pigment-related. Cinderella and Tiana patterns have respectively low and high melanin content in skin and eyes; Cinderella has white skin and blue eyes (low melanin content; LMC) and Tiana has dark skin and eyes (high melanin content; HMC). Comparative investigation between frequencies of genetic variants in Cinderella-Like versus Tiana-Like subjects may indicate which polymorphic variant is associated with melanin synthesis in skin and eyes. Coordinately, studies with Snow White-Like subjects may be informative to reveal any tissue-specific expression, since these individuals have both white skin (LMC) and dark eyes (HMC). Based on allele frequencies of different human popullations, allele ?L? was used for the alleles associated with low melanin content populations (Cinderela-like subjects), and allele ?H? was used for the alleles associated with high melanin content populations (Tiana-like subjects). Allelic distribution of eight SNPs showed that 100% of Cinderella- like subjects (N= 73) had less than eight H alleles, and 82% of Tiana-Like subjects (N= 61) had eight or more H alleles. The AUC (Area Under the Receiver Operating Characteristic Curve) value was 0.99, and the calculation of PGL (Pathway Genetic Load) and GP (Genetic Probability) showed that the SNP set presented 93% and 91% concordance between DNA genotype and phenotypes, respectively. Factorial discriminant analyses (FDA) performed in the Snow White group (light skin and dark eyes; N= 116) showed an association between SNPs rs16891982 (SLC45A2), rs8045560 (MC1R), rs1426654 (SLC24A5), rs2733832 (TYRP1), and rs1042602 (TYR) and the Cinderella cluster for skin phenotype, and an association between SNPS rs4778138 (OCA2), rs12913832 (HERC2), and rs916977 (HERC2) and the Tiana cluster for eye phenotype. Lastly, we studied 436 South Brazilian subjects with different skin and eye colors to construct a multinomial logistic regression model able to predict phenotype. The model was tested in 40 random subjects to measure the efficiency of the prediction. The data presented 93% concordance between the predicted and the observed phenotype, showing the successful phenotype prediction of the model in South Brazilian individuals. This is important since Brazil has an ethnically mixed population in which genomic structure may favor epistatic and pleiotropic effects not observed in populations that are more homogeneous. The analyses presented here are an important contribution to forensic DNA phenotyping scenario. / Os genes relacionados a caracter?sticas externamente vis?veis (EVC) apresentam grande import?ncia para a previs?o de cores de pele e olho em humanos. Neste estudo, testamos a capacidade de um conjunto de SNPs em genes relacionados ? s?ntese de pigmento melanina para prever cores de pele e olho em uma popula??o sul-brasileira. Inicialmente, utilizamos o modelo Cinderela - Tiana - Neve Branca para analisar a distribui??o al?lica de oito SNPs bial?licos associados ? produ??o da melanina. Os padr?es Cinderela e Tiana possuem respectivamente baixo e alto conte?do de melanina na pele e nos olhos; Cinderela tem pele branca e olhos azuis (baixo conte?do de melanina, LMC) e Tiana tem pele e olhos escuros (alto conte?do de melanina, HMC). A investiga??o comparativa entre frequ?ncias de variantes gen?ticas em indiv?duos com Cinderela-Like versus Tiana-Like pode indicar qual variante polim?rfica est? associada ? s?ntese de melanina na pele e nos olhos. De forma coordenada, os estudos com indiv?duos Branca de Neve-Like podem ser informativos para revelar a express?o tecido-espec?fica, uma vez que esses indiv?duos t?m pele branca (LMC) e olhos escuros (HMC). Com base em frequ?ncias de alelos de diferentes popula??es humanas, o nome alelo "L" foi utilizado para os alelos associados a popula??es de baixo conte?do de melanina (indiv?duos Cinderela-Like) e o nome alelo "H" foi utilizado para os alelos associados a popula??es de alto conte?do de melanina (Indiv?duos Tiana-Like). A distribui??o al?lica de oito SNPs mostrou que 100% de indiv?duos com Cinderela-Like (N= 73) tinham menos de oito alelos H e 82% de indiv?duos Tiana-Like (N= 61) tinham oito ou mais alelos H. O valor AUC (Area Under the Receiver Operating Characteristic Curve) foi de 0,99 e o c?lculo de PGL (Pathway Genetic Load) e GP (Probabilidade Gen?tica) mostrou que o conjunto de SNP apresentou concord?ncia de 93% e 91% entre gen?tipo e fen?tipo, respectivamente. As an?lises discriminantes fatoriais (FDA) realizadas no grupo Branca de Neve-Like (pele clara e olhos escuros; N= 116) mostraram associa??o positiva entre SNPs rs16891982 (SLC45A2), rs8045560 (MC1R), rs1426654 (SLC24A5), rs2733832 (TYRP1) e rs1042602 (TYR) e o cluster de Cinderela para o fen?tipo da pele, e associa??o positiva entre SNPS rs4778138 (OCA2), rs12913832 (HERC2) e rs916977 (HERC2) e o cluster Tiana para o fen?tipo dos olhos. Por fim, estudamos 436 sujeitos sul-brasileiros com diferentes cores de pele e de olhos para construir um modelo de regress?o log?stica multinomial capaz de prever o fen?tipo. O modelo foi testado em 40 indiv?duos aleat?rios para medir a efici?ncia da predi??o. Os dados apresentaram concord?ncia de 93% entre o fen?tipo previsto e observado, mostrando a previs?o fenot?pica bem-sucedida do modelo em indiv?duos sul-brasileiros. Isso ? importante, uma vez que o Brasil tem uma popula??o etnicamente mista, na qual a estrutura gen?mica pode favorecer os efeitos epistaticos e pleiotr?picos n?o observados em popula??es mais homog?neas. As an?lises aqui apresentadas s?o uma importante contribui??o para a ?rea da fenotipagem por DNA.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2018-09-24T17:51:40Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) tatiana_marques_bittencourt.pdf: 1215751 bytes, checksum: c3aad8d702755f268d177c8cafafe365 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-10-01T18:31:44Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) tatiana_marques_bittencourt.pdf: 1215751 bytes, checksum: c3aad8d702755f268d177c8cafafe365 (MD5) / Made available in DSpace on 2018-10-01T18:31:44Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) tatiana_marques_bittencourt.pdf: 1215751 bytes, checksum: c3aad8d702755f268d177c8cafafe365 (MD5) Previous issue date: 2018 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O gr?o seco de destilaria com sol?veis (DDGS) ? um coproduto da ind?stria de etanol do milho e pode ser utilizado na alimenta??o animal, como substituto do farelo de soja. Foram realizados dois experimentos com o objetivo de avaliar diferentes n?veis de inclus?o de DDGS de milho na dieta de codornas e galinhas poedeiras, sobre o desempenho, qualidade dos ovos, pigmenta??o da gema e an?lise econ?mica. No experimento 1, foram utilizadas 210 codornas poedeiras (Coturnix japonica) com 23 a 31 semanas de vida, distribu?das em delineamento inteiramente casualizado com cinco tratamentos e seis repeti??es, com sete aves por unidade experimental. No experimento 2, foram 150 galinhas poedeiras (Hisex Brown) com 54 a 62 semanas de vida, distribu?das em delineamento interiramente casualizado com cinco tratamentos e seis repeti??es, com cinco aves por unidade experimental. Os tratamentos utilizados nos dois experimentos foram: ra??o controle; inclus?o de 5% de DDGS; inclus?o de 10% de DDGS; inclus?o de 15% de DDGS e inclus?o de 20% de DDGS. As vari?veis avaliadas foram: consumo de ra??o, convers?o alimentar por d?zia e massa de ovos, produ??o de ovos, peso dos ovos, peso da gema, alb?men, varia??o do peso corporal, viabilidade das aves, gravidade espec?fica, colora??o da gema, componente do ovo e an?lise econ?mica. No experimento 1, para as vari?veis consumo individual, convers?o alimentar por massa de ovos, taxa de postura, gravidade espec?fica e e colora??o da gema pelo leque colorim?trico houve efeito com a inclus?o do DDGS (P<0,05), para as demais vari?veis n?o houve efeito significativo. No experimento 2, para as vari?veis colora??o da gema pelo leque colorim?trico e colorimetro digital (L* e a*) houve efeito linear com a inclus?o do DDGS (P<0,05), para as demais vari?veis n?o foi observada diferen?a significativa. Quanto ? an?lise econ?mica, o DDGS pode ser considerado um alimento alternativo pelo baixo custo (R$). O DDGS de milho tem o potencial pigmentante na gema do ovo, fazendo com que apresente mais amarela sem influenciar o desempenho e a qualidade dos ovos das galinhas e codornas poedeiras, al?m de ser um produto mais econ?mico. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2018. / Dry distillers grain with Solubles (DDGS) is a Coproduct of ethanol corn industry and can be used in animal nutrition, as a substitute for soybean meal. Two experiments were conducted to evaluate different levels of inclusion of DDGS of cornmaize in the diet of laying hens and Japanese quail, about the performance, eggs quality, yolk pigmentation and economic analysis. In experiment 1, 210 were used laying quail (Coturnix japonica) with 23 to 31 weeks of life, distributed in completely randomized design with six treatments and five replicates, with seven quail per experimental unit. In experiment 2, were 150 laying hens (Hisex Brown) with 54 to 62 weeks of life, distributed in entirely randomized design with six treatments and five replicates, with five birds per experimental unit. The treatments used in two experiments were: T1: ration control; T2: inclusion of 5% of DDGS; T3: inclusion of 10% of DDGS; T4:15% inclusion of DDGS; T5: inclusion of 20% of DDGS. The variables evaluated were: feed intake, feed conversion per dozen and mass of eggs, egg production, egg weight, weight variation, viability of birds, gravity specifies, coloring of the yolk, egg component and economic analysis. The data were subjected to analysis by linear and quadratic regression models, the 5% probability. In experiment 1, to the individual consumption and feed conversion ratio by mass of eggs there was quadratic effect with the inclusion of DDGS (P < 0.05), for gravity and egg weight, egg yolk, and gem coloration for range and colorimetric digital colorimeter (b *) effect with the inclusion of DDGS (P < 0.05), for the other variables had no significant difference. In experiment 2, for the variables weight of yolk, albumen and bark, and gem coloration for range and colorimetric digital colorimeter (L *) there was no linear effect with the inclusion of DDGS (P < 0.05), the other variables had no significant difference. As the economic analysis the DDGS can be considered an alternative food for low cost (R$). The DDGS of corn has the potential pigmentante in egg yolk, making present more yellow mainly to higher levels, without affecting the performance and eggs quality of hens and laying quails, as well as being a more economic product.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-01-06T16:36:08Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) thais_garcia_santos.pdf: 1190312 bytes, checksum: 9585de4f7a0418cbc740bc18909a228a (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-01-31T14:08:36Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) thais_garcia_santos.pdf: 1190312 bytes, checksum: 9585de4f7a0418cbc740bc18909a228a (MD5) / Made available in DSpace on 2017-01-31T14:08:36Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) thais_garcia_santos.pdf: 1190312 bytes, checksum: 9585de4f7a0418cbc740bc18909a228a (MD5) Previous issue date: 2016 / Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Banco do Nordeste do Brasil / A sobreviv?ncia de diversas esp?cies de peixes em cativeiro depende de sua adapta??o ?s condi??es ambientais, sendo essencial adequar par?metros como a luminosidade e cor ambiente. Com isso, objetivou-se, com o desenvolvimento de dois experimentos: com o primeiro, avaliar o desempenho de larvas de pacam? submetidos a duas cores de aqu?rio e dois n?veis de luminosidade e no segundo, avaliar o desempenho, o comportamento e pigmenta??o da pele de juvenis pacam? Lophiosilurus alexandri submetidos a diferentes n?veis de luminosidade. No primeiro experimento, conduzido por 25 dias, foram utilizadas 1200 larvas, distribu?dos em 4 tratamentos: aqu?rio branco com 0 Lux, aqu?rio branco com 88 Lux, aqu?rio preto com 0 Lux e aqu?rio preto com 88 Lux. Para o segundo experimento, conduzido por 75 dias, foram utilizados 160 juvenis de pacam?s submetidos a 4 luminosidades: 0 lux, 218 lux, 278 lux e 458 lux. Em ambos experimentos os animais foram alimentados quatro vezes ao dia, em ambiente com fotoperiodo de 12 horas de luz e 12 horas de escuro e aera??o constante. No primeiro experimento, indica-se para o cultivo de p?s larvas de pacam?s o uso de aqu?rios brancos e luminosidades entre 0 e 88 lux n?o afetam o desempenho dos peixes. J? no segundo experimento, as diferentes luminosidades n?o interferiram na pigmenta??o, crescimento e manteve baixo os n?veis de cortisol. Por?m recomenda-se o cultivo de juvenis de pacam?em ambiente sem luminosidade, pois diminui o consumo de ra??o e favorece a convers?o alimentar, sendo estas caracter?sticas desej?veis ? produ??o. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2016. / The survival of several species of fish in captivity depends on their adaptation to environmental conditions, it is essential adapt parameters such as brightness and color setting. It aimed to , with the development of two experiments : the first , to evaluate the performance of pacam? larvae submitted to two aquarium colors and two light levels and second , evaluate performance, behavior and skin pigmentation pacam? of juvenile Lophiosilurusalexandri subjected to different levels of brightness .In the first experiment, conducted for 25 days, they were used in 1200 larvae distributed in 4 treatments: White aquarium with 0 lux, white tank with 88 Lux, black tank with 0 lux and black tank with 88 Lux. For the second experiment was conducted for 75 days, they were used 160 pacam? juveniles subjected to 4 luminosities: 0 lux, 218 lux, 278 lux and 458 lux. In both experiments the animals were fed four times a day in photoperiod environment with 12 hour of light and 12 hours of dark and constant aeration. In the first experiment, it is indicated for post larvae pacam?s growing use of white and luminosities aquariums between 0 and 88 lux not affect fish performance. In the second experiment, the different luminosities not interfere in pigmentation, growth and kept the low cortisol levels. But it recommends the pacam? of juvenile culture in no light environment because it reduces feed intake and feed conversion favors, which are desirable characteristics to production.

Made available in DSpace on 2016-04-28T14:59:48Z (GMT). No. of bitstreams: 1 2008 - Thaiz Marinho Magalhaes Cedro.pdf: 1901514 bytes, checksum: e3897dc76edd8a3714a6678cf8139f8f (MD5) Previous issue date: 2008-08-29 / 1160 eggs were utilized in four experiments were conducted in Eggs Analyses Laboratory of the Institute of Animal Science of the Rural Federal University of Rio de Janeiro (UFRRJ) using commercial eggs produced by two groups of laying hens line Isa Brown with 33 weekold. In group 1, the birds were fed throughout their productive life with a basic diet of corn and soybean meal, while in group 2, since 22? weeks-old of the hens, was added to the basic diet 1.5% of marine algae substrate and 1.8% fish oil (production of enriched &#969;-3 eggs). In experiment 1, whose aim was to evaluate the internal and external quality of conventional and enriched &#969;-3 eggs stored at different temperatures, it was found that both types of eggs studied had similar characteristics of internal and external quality. Eggs stored for 21 days at 25?C had lower averages for Haugh unit and yolk index and higher averages of albumen pH and yolk pH when compared to other treatments related to temperature and period of storage. There were no significant differences in egg shell thickness, shell weight and shell percentage between the two types of eggs stored for 21 days, regardless of the temperature of storage. In experiment 2 was assessed the influence of the season on the quality of enriched &#969;-3 eggs stored in refrigerated environment. In this second issue was verify that the eggs collected in the summer showed eggs weight and internal and external quality aspects lower than those collected in winter. In this second issue was verify that the eggs collected in the summer showed eggs weight and internal and external quality aspects lower than those collected in winter. In this second issue was verify that the eggs collected in the summer showed eggs weight and internal and external quality aspects lower than those collected in winter. The storage affected negatively the eggs weight, Haugh unit and yolk index, but there was no effect on the shell thickness. In experiment 3 was compared the fatty acids levels and relationships in conventional and enriched &#969;-3 eggs. The enriched &#969;-3 eggs showed total levels of &#969;-3 polyunsaturated (1839 mg/100g of yolk) and monounsaturated fatty acids (10,744 mg/100g of yolk) higher than those of conventional eggs (927 and 7997 mg/100g of yolk, respectively). Relations between polyunsaturated/saturated fatty acids (P/S) and between &#969;-6/&#969;-3 of enriched eggs were close to the ideal estimated for human consumption (1.10 and 3.00, respectively). The conventional eggs had total levels of saturated (8740 mg/100g of yolk) and &#969;-6 polyunsaturated fatty acids (9600 mg/100g of yolk) significantly higher than the enriched &#969;-3 eggs (6640 and 5510 mg/100g of yolk, respectively). In Experiment 4, three people individually assessed the intensity of yolk pigmentation with a color range. It was found that conventional eggs had the yolk less pigmented enriched &#969;-3 ones. The storage at different temperatures caused the appearance of dark spots in the egg yolks enriched. Based on the results obtained in experimental conditions used it was found that enriched &#969;-3 eggs showed high levels of &#969;-3 polyunsaturated fatty acids, appropriate balance of the relations between P/S and between &#969;-6/&#969;-3 and good characteristics of internal and external quality, thus those eggs can be considered an excellent alternative food for those consumers concerned about eating healthy diets. / Foram utilizados 1160 ovos comerciais em quatro experimentos realizados no Laborat?rio de An?lises de Ovos do Instituto de Zootecnia da Universidade Federal Rural do Rio de Janeiro (UFRRJ) produzidos por dois grupos de poedeiras da linhagem Isa Brown com 33 semanas de idade. No grupo 1, as aves foram alimentadas durante toda vida produtiva com ra??o a base de milho e farelo de soja (produ??o de ovos convencionais), enquanto que no grupo 2, ? partir da 22? semana de idade das aves, foi acrescentado a ra??o b?sica 1,5% de substrato de algas marinhas e 1,8% de ?leo de peixe (produ??o de ovos enriquecidos com &#969;-3). No experimento 1, cujo objetivo foi avaliar a qualidade interna e externa de ovos comerciais convencionais e enriquecidos com &#969;-3 armazenados em diferentes temperaturas, foi verificado que os dois tipos de ovos estudados apresentaram caracter?sticas de qualidade interna e externa semelhantes. Os ovos armazenados por vinte e um dias a 25?C apresentaram menores m?dias para unidade Haugh e ?ndice de gema e maiores para pH do alb?men e da gema quando comparado aos demais tratamentos relacionados a temperatura e per?odo de armazenamento. N?o foram observadas diferen?as significativas para espessura, percentual e peso da casca entre os dois tipos de ovos estocados por vinte e um dias, independentemente da temperatura de armazenamento. No experimento 2 foi avaliada a influ?ncia da esta??o do ano sobre a qualidade de ovos enriquecidos com &#969;-3 armazenados em ambiente refrigerado. Neste segundo ensaio foi observado que os ovos coletados no ver?o apresentaram peso, qualidade interna e externa inferior ao daqueles coletados no inverno. Com rela??o ao armazenamento, foi verificado efeito negativo sobre o peso, unidade Haugh e ?ndice de gema, mas n?o houve efeito sobre a espessura da casca destes ovos. No experimento 3, comparou-se os n?veis de ?cidos graxos e suas rela??es em ovos comerciais convencionais e enriquecidos com &#969;-3 e foi verificado que os ovos enriquecidos com &#969;-3 apresentaram teores totais de ?cidos graxos (AG) poliinsaturados da s?rie &#969;-3 (1839 mg/100g de gema) e de AG monoinsaturados (10744 mg/100g de gema) significativamente superiores aos dos ovos convencionais (927 e 7997 mg/100g de gema, respectivamente). As rela??es entre os ?cidos graxos poliinsaturados/saturados (P/S) e entre &#969;-6/&#969;-3 dos ovos enriquecidos foram pr?ximas ao ideal estimado para o consumo humano (1,10 e 3,00; respectivamente). Os ovos convencionais apresentaram teores totais de AG saturados (8740 mg/100g de gema) e de AG poliinsaturados da s?rie &#969;-6 (9600 mg/100g de gema) significativamente superiores aos dos ovos enriquecidos &#969;-3 (6640 e 5510 mg/100g de gema, respectivamente). No experimento 4, tr?s pessoas individualmente avaliaram a intensidade de pigmenta??o da gema com o aux?lio de um leque colorim?trico. Foi verificado que os ovos convencionais apresentaram gema menos pigmentada que os ovos enriquecidos com &#969;-3. O armazenamento em diferentes temperaturas provocou o aparecimento de manchas escuras nas gemas dos ovos enriquecidos. Com base nos resultados obtidos nas condi??es experimentais utilizadas concluiu-se que os ovos enriquecidos com &#969;-3 apresentaram elevados n?veis de AG poliinsaturados da s?rie &#969;-3, balan?o adequado das rela??es entre &#969;-6/&#969;-3 e P/S e boas caracter?sticas de qualidade interna e externa, sendo desta forma, considerados uma excelente alternativa de alimento para aqueles consumidores preocupados em ingerir dietas mais saud?veis.