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Thesis (M.A. Agricultural Administration (Pasture Science)) -- University of Limpopo, 2014 / In the summer rainfall areas of South Africa small scale farmers, as well as commercial farmers experience low animal production due to a lack of good quality roughage. The nutritional value of the rangeland cannot maintain livestock during autumn and winter. Producing winter fodder could be expensive and literature showed that planting fodder radish and turnip might be a cheap relative option (not in sweet veld). Winter supplementation contributes largely to high input costs in livestock production, which can make this enterprise uneconomically. For this reason alternative winter feeding strategies should be investigated, like the use of Brassica and Raphanus species for feed supply, of high quality, in winter. Two Localities [Syferkuil, (University of Limpopo’s experimental farm (Limpopo) and Dewageningsdrift, (Hygrotech Experimental farm (Gauteng)] were used for this research project. On the two localities three different factors were tested:  Three planting dates (February, March and April)  Three cultivars (Nooitgedacht fodder radish, Forage star turnip (not on Syferkuil) and Mammoth purple top turnip)  Three cutting frequencies (first cut10 weeks after planting + regrowth; first cut 14 weeks after planting + regrowth and 18 weeks after planting, no regrowth). Samples (for dry matter production and nutritional value analysis) were collected at both localities as per cutting frequency treatments during the 2007 growing season. The samples were used to evaluate the influence of the mentioned treatments on total dry matter production, nutritional value, leaf production and tuber production of the three cultivars. At Syferkuil the DM production Nooitgedacht fodder radish was higher (5.23 to 5.9 t/ha) than that of Mammoth purple top turnip (3.24 t/ha) when planted in February. The same trend was seen during the March planting date (4.7 t/ha and 3.6 t/ha respectively for 18 W treatment). During the April planting date the highest production was higher (5.07 t/ha and 5.13 t/ha respectively) than that of the March iv planting date. The 10 Weeks + Re-growth cutting treatment resulted in general in the lowest production. At Dewageningsdrift (Gauteng) Nooitgedacht fodder radish produced the highest of all three cultivars at the 18 Weeks treatment, with the highest when planted in March (7.67 t/ha), 5.5 t/ha when planted in April and 5.3 t/ha when planted in February. For the rest of the treatments the DM production of Nooitgedacht varied between 2.9 t/ha and 4.6 t/ha. The highest DM production of Forage star turnip was 3.01 t/ha (10 W+R, February planting date), 1.35 t/ha (14 Weeks + Re-growth, March planting date) and 2.34 t/ha (18 Weeks, April planting date). The highest DM production of Forage star turnip was 2.96 t/ha (18 Weeks, February planting date), 2.59 t/ha (14 Weeks + Re-growth, March planting date) and 4.1 t/ha (18 Weeks, April planting date). An estimation of the grazing/feeding potential of the different cultivars, at different planting dates and defoliation/cutting treatments, was calculated by using the leave and tuber production (variable criteria) from each treatment. The period from the initial cut to the last regrowth cut was a second variable criterion that was used. The third criterion (non-variable) was the standard norm that the daily intake of a matured livestock unit (MLU) of 450 kg is 10 kg. According to the results the following example of a combination of treatments can be used to maintain ± 10 MLU/ha for the longest period in the winter in Limpopo: Plant 1.1 ha Nooigedacht radish in February, utilize from ± 27 April to 22 June, Plant 2.4 ha Nooigedacht radish in April, utilize from ± 22 June to 27 August, Plant 0.9 ha Mammoth purple top in April, utilize from ± 20 August to 3 Oct According to the results the following example of a combination of treatments can be used to maintain ± 10 MLU/ha for the longest period in the winter in Gauteng: Plant 2.1 ha Forage star turnip in February, utilize from ± 12 April to 13 June, Plant 1.7 ha Mammoth purple top in February, utilize from ± 7 June to 28 July, Plant 1.5 ha Forage star turnip in April, utilize from ± 18 July to 29 August, Plant 2.1 ha Forage star turnip in April, utilize from ± 17 August to 4 Oct.

Thesis (M.S.)--Auburn University, 2008. / Abstract. Vita. Includes bibliographical references.

No description available.

Eucalyptus spp. and hybrids dominate the global plantation forestry industry, and vegetative propagation through cuttings is the preferred method for their commercial use. However, the cuttings of some species and hybrids show recalcitrance to rooting. The first aim of this study was to improve percentage rooting of three clones of E. grandis x E. nitens (Clones 1, 2 and 3) identified by a commercial nursery as having variable rooting abilities. The second was to relate their rooting responses as cuttings to their rooting responses in vitro. Minicuttings (3.5 – 4 cm in length) (hereafter referred to as cuttings) were subjected to commercial nursery propagation practices. Initial results revealed that in the absence of exogenous plant growth regulators (PGRs), soft (juvenile, thin diameter) cuttings survived (87 – 95%) and rooted (29 – 32%) significantly better than hard (mature, thick diameter) ones (62 – 71% survival and 2 – 8% rooting). This validated the use of soft cuttings by the nursery and all subsequent studies were conducted with soft cuttings. The other nursery practice of applying the commercial rooting powder Seradix 2 (3 g kgˉ¹ indole-3-butyric acid [IBA]) adversely affected the survival and subsequent rooting of cuttings of Clones 1 and 2. Ensuing studies investigated: 1) the effect of mode of IBA application (powder vs. liquid); 2) concentrations of Seradix (0, 0.5, 1, 2 and 3 g kgˉ¹ IBA), applied at initial placement of cuttings and two weeks later; and 3) the influence of season on the survival and subsequent rooting of cuttings. Results showed that regardless of the mode of application, IBA significantly reduced percentage survival and rooting in cuttings of Clones 1 and 2. The delayed application of Seradix, two weeks after cuttings were initially set, resulted in a higher percentage survival and rooting than when cuttings were supplied with Seradix at initial placement. Nevertheless, the best survival for Clones 1, 2 and 3 (95%, 99% and 71%, respectively) and rooting (83%, 64% and 47%, respectively) occurred in the absence of Seradix. In addition, the survival and rooting of cuttings were seasonally variable, with particularly low rooting during winter (e.g. for Clone 1, 32%) when compared with summer (e.g. for Clone 1, 83%). Shoots from all the clones were multiplied in vitro, followed by elongation on either of two media (E1= kinetin, α-naphthalene acetic acid [NAA] and IBA; E2 = kinetin and indole-3-acetic acid [IAA]), and then rooting on 0, 0.1 or 1.0 mg 1ˉ¹ IBA. The latter were selected to typify the range of Seradix concentrations used for the cuttings (i.e. no IBA, low and high IBA concentrations). For all three clones, shoots elongated on E1 or E2 displayed high survival (> 80%) but failed to root without IBA in the rooting medium. For Clones 1, 2 and 3 the best in vitro survival (80%, 100% and 100%, respectively) and rooting (40%, 75% and 40%, respectively) occurred when shoots were elongated on E2 and rooted on 0.1 mg 1ˉ¹ IBA. However, 1.0 mg 1ˉ¹ IBA in the rooting medium severely inhibited survival (0 – 50%), irrespective of the clone or the elongation treatment used. Overall, cuttings demonstrated the best survival and rooting in the absence of exogenous IBA, which suggested that sufficient endogenous auxin was present within the shoots for successful root induction. The application of exogenous IBA may have disrupted the cuttings’ endogenous PGR balance resulting in an inhibition of survival and rooting. In vitro shoots required a low concentration of IBA (0.1 mg 1ˉ¹) in order to counteract the antagonistic effect of cytokinins that were supplied during the multiplication and elongation culture stages, and promote rhizogenesis. Essentially, both cuttings and in vitro shoots demonstrated adverse survival and rooting responses when subjected to excessively high IBA concentrations. / Thesis (M.Sc.)-University of KwaZulu-Natal, Durban 2013.

Copies no. 3007841664 and 3007841665 are photocopies of the original. / Thesis (MScAgric)-- University of Stellenbosch, 2003. / ENGLISH ABSTRACT: Buchu (Agathosma betulina and A. crenulata) are grown commercially as an aromatic crop and are endemic to the Western Cape of South Africa. Poor rooting of cuttings have limited the development of superior clones. Under standard mist bed conditions terminal, sub-terminal or basal stem cuttings were taken from March to August. When not treated with an auxin, rooting percentages of between 20 and 25 were obtained. Rooting percentages increased to between 40 and 45 after treatment with 500-1000 ppm indolebutyric acid (lBA). Substituting lBA with naphthaleneacetic acid (NAA) did not improve rooting. There was a tendency for cuttings with fewer than four leaf pairs to give lower rooting percentages. Plants of Agathosma betulina x A. crenulata, grown in Paarl, and A. betulina, grown in Piketberg, were used as source plants for making cuttings. Paarl plants were shaded with 80 percent shade and Piketberg plants with 60 or 80 percent shade respectively from February to October 2002. Plants in full sun served as a control. Plants were pruned back initially in February and then two months before samples were taken in March, June, August and October at both locations. New shoots were used as cuttings. Terminal cuttings for rooting and for carbohydrate analyses were collected on four different dates (March, June, August and October). Cuttings were treated with 500 ppm indolebuteric acid (lBA) and placed in misting beds with bottom heating (18-25°C) for a period of three months. Shading reduced rooting of cuttings from the Paarl plants. However, it did not significantly increase rooting of cuttings taken from Piketberg plants. Rooting percentage was the highest in August (43%) for cuttings from sun grown plants in Paarl. No consistent relationship between, respectively, dry mass or carbohydrate content of cuttings and rooting could be established. Terminal current years' growth, taken from Agathosma crenulata x A. betulina (hybrid) softwood cuttings, collected in January 2002, were extracted with methanol and fractioned by thin layer chromatography (Silica gel) in isopropanol: acetic acid: water (4: 1:1 v/v). The chromatographs were divided in ten fractions and were bio-assayed for a rooting co-factor with the mung bean rooting test. Extracts from buchu cuttings showed significant activity at the Rf values of co-factor 3. Co-factors 1,2 and 4 do not seem to be present in significant quantities. However, co-factors with Rf values different from previous reported values were present in significant quantities. No inhibition was found in buchu. In fact, all Rf values stimulated rooting. / AFRIKAANSE OPSOMMING: Boegoe (Agathosma betulina x A. crenulata) word kommersieël verbon as 'n aromatiese gewas en is endemies tot die Wes-Kaap. Die ontwikelling van superieure klonale materiaal word beperk deur swakbeworteling. Terminale, sub-terminale en basale steggies is gesnyonder standaard misbed toestande van Maart tot Augustus. Beworteling was tussen 20 en 25 persent as geen ouksien gebruik word nie. As indolebottersuur (IBS) gebruik word tussen 500-1000 dpm, verhoog die bewortelingspersentasie tot tussen 40 en 45 persent. Die gebruik van naftaleen asynsuur (NAS) in plaas van IBS het nie beworteling verbeter nie. Daar was a tendens dat steggies wat minder as vier blaarpare gehad het 'n verlaging in bewortelingspersentasies gehad het. Plante van Paarl, A. betulina x A. crenulata, en Piketberg, A. betulina, is gebruik as plantmateriaal vir steggies. Plante in die Paarl was onder 80 persent skadu geplaas en plante in Piketberg onder 60 en 80 persent skadu van Februarie tot Oktober 2002. Plante in vol son was as 'n kontrole gebruik. Plante was eers in Februarie teruggesny en dan weer twee mande voor monsters geneem is. Die monsters is in Maart, Junie, Augustus en Oktober geneem in beide liggings. Terminale steggies is vier keer ingesamel (Maart, Junie, Augustus en Oktober) vir beworteling en koolhidraat analises. Die steggies is met 500 dpm IBS behandel. Daarna is die steggies vir drie maande in die misbed geplaas met bodem-verhitting (18- 25°C). Dit is gevind dat die gebruik van skadu die beworteling in Paarl verminder het alhoewel die beworteling in Piketberg nie beduidend beinvloed is nie. Die hoogste bewortelingspresentasies is waargeneem in Augustus (43%) in Paarl van plante wat in vol son was. Geen verband tussen onderskeidelik die droe massa of koolhidraat inhoud en beworteling kon gevind word nie. Terminale steggies van dieselfte jaar se groei van Agathosma betulina x A. crenulata (hibried) is in Januarie 2002 ingesamel. Die materiaal is geëkstraheer en gefraksioneer deur dunlaag kromatografie in isopropanol: asynsuur: water (4: 1:1 v/v). Die kromograaf is in 10 fraksies verdeel. Die fraksies was bioassaieer VIr beworteling ko-faktore met die mungboontjie bewortelingstoets. Die ekstrakte van boegoe het beduidende aktiwiteit by die Rf waardes van ko-faktor 3 getoon. Ko-faktore 1, 2 en 4 is nie in groot genoeg hoeveelhede waargeneem nie. Ko-faktore, wat nie voorheen gevind is nie, is waargeneem in beduidende hoeveelhede. Geen inhibitors is in boegoe gevind nie en al die getoetste ko-faktore het beworteling gestimuleer.

Made available in DSpace on 2014-06-11T19:26:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-10-06Bitstream added on 2014-06-13T19:34:19Z : No. of bitstreams: 1 fraguas_cb_dr_botfca.pdf: 1400606 bytes, checksum: e3a3b343e1fdf633f6e3bb2a35833a4e (MD5) / Este trabalho teve como objetivo avaliar os efeitos da aplicacao exogena de poliaminas e TIBA (antiauxina) na micropropagacao e anatomia foliar de plantulas de abacaxizeiro eIAC Gomo-de-mel f e analisar os teores de poliaminas endogenas, atividade de peroxidase, proteina, IAA-oxidase e a provavel relacao com as diferentes fases da micropropagacao. Inicialmente, retiraram-se as gemas da coroa de frutos sadios e a assepsia foi realizada com hipoclorito de sodio comercial (2 a 2,5% de cloro ativo) a 30% por 20 minutos e lavadas por 3 vezes em agua destilada e autoclavada. Em seguida, inocularam-se as gemas em meio MS solido contendo diferentes combinacoes de BAP (0; 0,5; 1,0 e 1,5 mg L-1) e NAA (0; 0,5 e 1,0 mg L-1). Apos 60 dias, foram selecionados os melhores tratamentos para proliferacao das brotacoes, que foram individualizadas e transferidas para estes meios por mais trinta dias, em meio MS liquido. Plantas oriundas do experimento anterior tiveram as folhas cortadas e apenas segmentos de 1 cm foram inoculados em meio MS liquido contendo os diferentes tratamentos: T1-MS, T2-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA, T3-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM SPD, T4-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM SPM, T5-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM PUT, T6-MS + 10 mM SPD, T7-MS + 10 mM SPM e T8-MS + 10 mM PUT. Na ultima fase, segmentos foram inoculados em meio MS liquido contendo os tratamentos: T1-MS, T2-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA, T3-MS + 0,5 ÊM TIBA, T4-MS + 1,0 ÊM TIBA, T5-MS + 2,0 ÊM TIBA, T6-MS + 4,0 ÊM TIBA, T7-MS + 8,0 ÊM TIBA e T8-MS + 16,0 ÊM TIBA. Apos, as plantas foram aclimatizadas em substrato Plantmax e houve 100% de sobrevivencia, independente do tratamento. Foram realizados cortes histologicos foliares para estudar a influencia das poliaminas e TIBA na anatomia foliar... / The effects of exogenous polyamines and TIBA (antiauxin) application in the micropropagation, leaf anatomy of pineapple plants IAC Gomo-de-mel and the contents of endogenous polyamines, peroxidase activity, protein, IAA-oxidase and probable relationship with the different micropropagation phases were studied. Initially, the axillary bud explants were excised from the crown of healthy fruits and the asepsis was accomplished with sodium hypochloride (2 to 2,5% of active chlorine) 30% for 20 minutes and washed 3 times in distilled and autoclaveted water. After, the axillary buds were inoculated in MS solid medium containing the different BAP (0; 0,5; 1,0 and 1,5 mg L-1) and NAA (0; 0,5 and 1,0 mg L-1) combinations. After 60 days, the best treatments were selected for shoot proliferation that were individualized and transferred to these media for more 30 days, in MS liquid medium. Plants from the previous experiment had the leaves cut and just 1 cm segments were inoculated in MS liquid medium containing the different treatments: T1-MS, T2-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA, T3-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM SPD, T4- MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM SPM, T5-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM PUT, T6-MS + 10 mM SPD, T7-MS + 10 mM SPM and T8-MS + 10 mM PUT. In the last phase, segments were inoculated in MS liquid medium containing the treatments: T1-MS, T2-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA, T3-MS + 0,5 mM TIBA, T4-MS + 1,0 mM TIBA, T5-MS + 2,0 mM TIBA, T6-MS + 4,0 mM TIBA, T7-MS + 8,0 mM TIBA and T8-MS + 16,0 mM TIBA. After, the plants were acclimatizated in Plantmax substrate and there were 100% survival, independent of the treatment. Leaf cuts were made to study polyamines and TIBA influence in the leaf anatomy. The highest shoot number wasobserved with 1,0 mg L-1 BAP + 0,5 mg L-1 NAA, however with hyperhydricity...(Complete abstract click electronic access below)

A maioria das fruteiras nativas pertencem a família Myrtaceae, que apresentam como principal potencialidade a comercialização seus frutos. Apesar da grande aceitação dos frutos destas fruteiras, ainda é necessário a criação de pomares comerciais, pois prevalece-se o extrativismo. Para iniciar qualquer pomar, o primeiro ponto está na escolha da planta matriz, devendo esta apresentar características de superioridade quando comparadas aos outros genótipos. A partir disso, deve-se proceder na escolha do método que possibilite produzir quantidade de mudas satisfatória e de preferência sem perder as características da planta mãe. Para isso adota-se técnicas assexuadas, com opção para enxertia, estaquia e alporquia. Estas técnicas quando testadas com fruteiras nativas, mostraram-se limitantes em diversos aspectos, devendo-se testar outra que poderá ser recomendada para uso, principalmente naquelas de maior potencialidade como jabuticabeiras, pitangueiras, sete capoteiro e araçazeiro amarelo. O objetivo deste trabalho foi testar o uso da propagação assexuada por mini-estaquia nestas fruteiras, de acordo com época de coleta, comprimento da mini-estaca e concentração de AIB, bem como, relacionar tais resultados de rizogênese com triptofano extraído em determinados períodos. O trabalho foi realizado na Universidade Tecnológica Federal do Paraná - Câmpus Dois Vizinhos. As coletas foram realizadas bimestralmente. Foram preparadas mini-estacas com 6 ou 8 cm, com par de folhas reduzido a 25% do tamanho original. As mini-estacas tiveram sua base imersa em solução líquida de ácido indol-butírico (AIB), nas concentrações de 0, 3000 e 6000 mg L-1 e em seguida foram colocadas em tubetes contendo substrato comercial. O delineamento experimental foi inteiramente casualizado com fatorial 2 x 3 x 6 (comprimento de estaca x concentração de AIB x época do ano), com quatro repetições, sendo a unidade experimental variou de acordo com a quantidade de brotações obtidas em tais períodos. Aos 120 dias da implantação de cada coleta foram avaliados o enraizamento e calogênese (%), número médio de raízes por mini-estaca e comprimento médio das raízes. Após 60 dias destas análises avaliou-se a sobrevivência das mini-estacas enraizadas pós-plantio. Também avaliou-se a produção de mini-estacas de cada tamanho em cada época do ano. Ao final do experimento avaliou-se o percentual de sobrevivência das mudas matrizes. Para análise de triptofano utilizou-se materiais de ramos, folhas e ramos com folhas, retirados dos materiais utilizados para produção de mini-estacas. Recomenda-se para jabuticabeira híbrida o uso de mini-estacas com oito centímetros, tratadas com 6000 mg L-1 de AIB e coletadas em junho. Para jabuticabeira de cabinho e araçazeiro o período de coleta para propagação por mini-estaquia destas deve ser em agosto, independente da concentração de AIB e comprimento da mini-estaca. Com a jabuticabeira sabará e sete capoteiro é importante para obtenção de resultados mais satisfatórios a coleta sendo realizada tanto em outubro quanto em dezembro, com mesma independência dos outros níveis testados nos outros fatores. Contudo, para sete capoteiro deve-se testar outras técnicas para aumentar a eficiência da propagação. E com pitangueira recomenda-se coletar em junho, porém nas de 6 cm aplicação de 3000 mg L-1 de AIB e de 8 cm com 6000 mg L-1 de AIB. / The most native fruit trees are belonging to Myrtaceae family, which it have as main marketing potential their fruit. Despite the wide acceptance of the fruits of these native fruit cultura, the establishment of commercial orchards is still necessary, because if it prevails extraction in the forest. To start the cultivo in the orchard, the first point is on the mother plant choice, which should provide superior characteristics when compared to other genotypes. Then, it is necessary to choose the method to can produce satisfactory amount of seedlings and preferably without it to lose the mother plant characteristics. For this, it adopts the asexual thechniques, with option for grafting, cuttings and air layering. These techniques when tested with native fruits tree, it proved limiting in theses results, with this, it should to test other it to recommend its use, especially, those fruit native of higher potential as jabuticaba tree, pitanga tree, sete capote tree and araça amarelo tree. The aim of this study was to test the use of asexual propagation through mini-cuttings in these native fruit trees, according to the time of collection, the mini-cutting length and concentration of IBA, as well as, it to relate the results of rooting with tryptophan extracted at certain times. The work was carried out at Universidade Tecnológica Federal do Paraná – Câmpus Dois Vizinhos, Brazil. The samples were collected each two months. The mini-cutting were prepared with 6 or 8 cm, with a pair of leaves reduced to 25% of the original size. The mini-cuttings had their base immersed in liquid solution of indole-butyric acid (IBA) in the concentrations of 0, 3000 and 6000 mg L-1 and then were placed in tubes containing commercial substrate. The experimental design was completely randomized with factorial 2 x 3 x 6 (mini-cutting length x IBA concentration x time of collection), with four replications, it being each plot varied according to the amount of shoots obtained by period time. After 120 days, the rooting and callus formation (%), average number of roots per mini-cutting and the average length of the roots were evaluated. After 60 days of these evaluations, the survival of mini-cuttings rooted after transplant was evaluated. It was evaluated also the production of mini-cuttings of each size in each period time. At the end of the experiment it was evaluated the percentage of survival of mother plantlets. For analysis of tryptophan was used materials branches, leaves and twigs with leaves, taken from the materials used for the production of mini-cutting. It was recommended for hybrid jabuticaba tree the use mini-cutting with eight cm, treated with 6000 mg L-1 of IBA and collected in June. For jabuticaba tree of cabinho and araça amarelo tree the period for propagation by mini-cuttings should be in August, regardless of IBA concentration and length of the mini-cutting. In the jabuticaba tree sabara and sete capote tree is important to obtain more satisfactory results realized the collect in October or December, with the same independence of other levels tested in other factors. However, for sete capote tree should test other techniques to increase the efficiency of propagation. And with pitanga tree recommended to the collection in June, but with 6cm the application of 3000 mg L-1 of IBA and 8 cm with 6000 mg L-1 of IBA.

Devido ao longo período juvenil que a jabuticabeira apresenta quando proveniente de sementes torna-se importante a utilização de outros métodos que minimizem esse problema. O objetivo deste trabalho foi estabelecer método eficiente para propagação vegetativa da jabuticabeira por enxertia, alporquia e estaquia. O trabalho foi realizado de 2011 a 2013, no Viveiro de Produção de Mudas, da UTFPR – Câmpus Dois Vizinhos (PR). O trabalho foi dividido em 4 experimentos de acordo com o método utilizado. No experimento 1 testou-se enxertia por meio de delineamento em blocos completamente casualizados, em fatorial 3 x 2 (espécie de porta-enxerto x tipo de enxertia), com 4 repetições, usando 10 plantas por parcela. Testou-se a compatibilidade de enxertia da jabuticabeira Plinia cauliflora sobre três espécies da família Myrtaceae como porta-enxertos (Eugenia uniflora L., E. involucrata DC e Plinia cauliflora), obtidas por sementes. Foram testados dois tipos de enxertia (fenda cheia e inglês com entalhe). O experimento 2 de alporquia, adotou o delineamento em blocos ao acaso, em fatorial 3 x 3 x 3 (época x concentração de AIB x tipo de embalagem), com 4 repetições, considerando-se o uso de 5 alporques por parcela. Testou-se as concentrações de 0, 2000 e 4000 mg.L-1 de AIB e os materiais de revestimento saco plástico transparente, saco plástico transparente revestido por papel alumínio e saco plástico preto. No experimento 3 de estaquia foi adotado o delineamento em blocos completamente casualizados, em fatorial 6 x 3 x 3 [época de coleta x técnica de condicionamento x concentração de ácido indol-3- butírico (AIB)], com 4 repetições, com 20 estacas por parcela. As estacas lenhosas foram coletadas bimestralmente, testando-se as técnicas de anelamento e estiolamento. Aplicou-se concentrações de AIB de 0; 3000 e 6000 mg L-1. No experimento 4 de estaquia, o delineamento experimental foi em blocos completamente casualizados, em fatorial 6 x 4 x 3 (época de coleta x embalagem de propagação x tamanho da estaca), com 4 repetições, considerando-se o uso de 15 estacas por parcela. As estacas foram retiradas bimestralmente, testando-se os tamanhos de 6 e 12 e 18 cm de comprimento e, quatro tipos de embalagens [bandejas plásticas revestidas com plástico preto, garrafa PET® com bordas de caixas de leite Tetra Pak® pretas, garrafas PET® e caixas de leite Tetra Pak® pretas]. Deve-se na enxertia da jabuticabeira Açú utilizar como porta-enxerto plantas da mesma espécie, tanto pelo método de fenda cheia como inglês com entalhe. A embalagem plástica transparente revestida com papel alumínio para cobertura do substrato mostrou-se promissora no processo de alporquia da jabuticabeira Açú, indicando-se sua realização no mês de abril. As concentrações de AIB testadas na alporquia não influenciaram a rizogênese adventícia dos ramos. As técnicas de anelamento utilizadas foram viáveis para a estaquia de jabuticabeira, recomendando-se realizadas nos meses de fevereiro e abril, com AIB. Recomenda-se ainda que o tempo dos procedimentos (anelamento e estiolamento) e a retirada dos ramos deva-se melhor analisado. Entretanto, a época de coleta, tipo de embalagem e o tamanho de estacas não influenciaram no enraizamento das estacas de jabuticabeira. / The jabuticaba fruit tree from seeds present long juvenile period becomes important to use other methods that will minimize this problem. The aim of this study was to establish efficient methods for jabuticaba fruit tree vegetative propagation by grafting, air layering and cuttings. The study was carried out at Nursery Sector of UTFPR - Câmpus Dois Vizinhos, Paraná State, Brazil at 2011 to 2013. The work was divided into four experiments according the propagation vegetative method used. The experiment 1 evaluated the grafting used the experimental design was blocks completely randomized, in factorial 3. x 2 (rootstock specie x grafting type), with 4 replications of ten grafts by plot. It was tested the jabuticaba fruit tree (Plinia cauliflora) grafting compatibility in three Myrtaceae species (Surinam cherry, native cherry and jabuticaba fruit tree) as rootstock. It was tested the cleft grafting and wedge graft. The experiment 2 tested the experimental design was blocks completely randomized, in factorial 3. x 3 x 3 (time x IBA concentration x packaging type), with 4 replications of five branches by plot. It was used in the cambial region IBA in the 0, 2000 and 4000 mg L-1 concentrations and the transparent plastic packaging, plastic packaging transparent coated foil or black plastic packaging as substrate packaging involved. The experiment 3 was used the experimental design was blocks completely randomized, in factorial 6. x 3 x 3 (time harvest x conditioning technical x IBA concentration), with 4 replications of twenty cutes by plot. The wood cuttings were harvested each two monts, it tested the girdling and etiolation. It was applied the IBA concentrations of 0, 3000 and 6000 mg L-1. The experiment 4 the experimental design was blocks completely randomized, in factorial 6. x 4 x 3 (time harvest x propagation bag x IBA concentration), with 4 replications of fifteen cutes by plot. The wood cuttings were harvested each two monts, it tested 6, 12 and 18 cm cutting sizes and, four packaging type (plastic trays involved with black plastic, PET® bottle with Ò black Tetra Pak® packaging, PET® bottle and black Tetra Pak® packaging). For Açú jabuticaba fruit tree must to use plants of same species as rootstock in the grafting, with the cleft grafting and wedge graft technicals. The plastic packaging transparent coated foil was promising for Açu jabuticaba fruit tree air layering, it wa recommend realized in April. The IBA concentrations did not influence the branches adventitious root formation. It was recommend that to use the girdling technique for jabuticaba fruit tree cutting in February and April months, with IBA. It was recommend that procedures time for girdling and shading and the branches harvest must be better analyzed. However, the harvest time, propagation bag and cutting length did not influence the jabuticaba fruit tree cutting rooting of jabuticabeira.

A maioria das fruteiras nativas pertencem a família Myrtaceae, que apresentam como principal potencialidade a comercialização seus frutos. Apesar da grande aceitação dos frutos destas fruteiras, ainda é necessário a criação de pomares comerciais, pois prevalece-se o extrativismo. Para iniciar qualquer pomar, o primeiro ponto está na escolha da planta matriz, devendo esta apresentar características de superioridade quando comparadas aos outros genótipos. A partir disso, deve-se proceder na escolha do método que possibilite produzir quantidade de mudas satisfatória e de preferência sem perder as características da planta mãe. Para isso adota-se técnicas assexuadas, com opção para enxertia, estaquia e alporquia. Estas técnicas quando testadas com fruteiras nativas, mostraram-se limitantes em diversos aspectos, devendo-se testar outra que poderá ser recomendada para uso, principalmente naquelas de maior potencialidade como jabuticabeiras, pitangueiras, sete capoteiro e araçazeiro amarelo. O objetivo deste trabalho foi testar o uso da propagação assexuada por mini-estaquia nestas fruteiras, de acordo com época de coleta, comprimento da mini-estaca e concentração de AIB, bem como, relacionar tais resultados de rizogênese com triptofano extraído em determinados períodos. O trabalho foi realizado na Universidade Tecnológica Federal do Paraná - Câmpus Dois Vizinhos. As coletas foram realizadas bimestralmente. Foram preparadas mini-estacas com 6 ou 8 cm, com par de folhas reduzido a 25% do tamanho original. As mini-estacas tiveram sua base imersa em solução líquida de ácido indol-butírico (AIB), nas concentrações de 0, 3000 e 6000 mg L-1 e em seguida foram colocadas em tubetes contendo substrato comercial. O delineamento experimental foi inteiramente casualizado com fatorial 2 x 3 x 6 (comprimento de estaca x concentração de AIB x época do ano), com quatro repetições, sendo a unidade experimental variou de acordo com a quantidade de brotações obtidas em tais períodos. Aos 120 dias da implantação de cada coleta foram avaliados o enraizamento e calogênese (%), número médio de raízes por mini-estaca e comprimento médio das raízes. Após 60 dias destas análises avaliou-se a sobrevivência das mini-estacas enraizadas pós-plantio. Também avaliou-se a produção de mini-estacas de cada tamanho em cada época do ano. Ao final do experimento avaliou-se o percentual de sobrevivência das mudas matrizes. Para análise de triptofano utilizou-se materiais de ramos, folhas e ramos com folhas, retirados dos materiais utilizados para produção de mini-estacas. Recomenda-se para jabuticabeira híbrida o uso de mini-estacas com oito centímetros, tratadas com 6000 mg L-1 de AIB e coletadas em junho. Para jabuticabeira de cabinho e araçazeiro o período de coleta para propagação por mini-estaquia destas deve ser em agosto, independente da concentração de AIB e comprimento da mini-estaca. Com a jabuticabeira sabará e sete capoteiro é importante para obtenção de resultados mais satisfatórios a coleta sendo realizada tanto em outubro quanto em dezembro, com mesma independência dos outros níveis testados nos outros fatores. Contudo, para sete capoteiro deve-se testar outras técnicas para aumentar a eficiência da propagação. E com pitangueira recomenda-se coletar em junho, porém nas de 6 cm aplicação de 3000 mg L-1 de AIB e de 8 cm com 6000 mg L-1 de AIB. / The most native fruit trees are belonging to Myrtaceae family, which it have as main marketing potential their fruit. Despite the wide acceptance of the fruits of these native fruit cultura, the establishment of commercial orchards is still necessary, because if it prevails extraction in the forest. To start the cultivo in the orchard, the first point is on the mother plant choice, which should provide superior characteristics when compared to other genotypes. Then, it is necessary to choose the method to can produce satisfactory amount of seedlings and preferably without it to lose the mother plant characteristics. For this, it adopts the asexual thechniques, with option for grafting, cuttings and air layering. These techniques when tested with native fruits tree, it proved limiting in theses results, with this, it should to test other it to recommend its use, especially, those fruit native of higher potential as jabuticaba tree, pitanga tree, sete capote tree and araça amarelo tree. The aim of this study was to test the use of asexual propagation through mini-cuttings in these native fruit trees, according to the time of collection, the mini-cutting length and concentration of IBA, as well as, it to relate the results of rooting with tryptophan extracted at certain times. The work was carried out at Universidade Tecnológica Federal do Paraná – Câmpus Dois Vizinhos, Brazil. The samples were collected each two months. The mini-cutting were prepared with 6 or 8 cm, with a pair of leaves reduced to 25% of the original size. The mini-cuttings had their base immersed in liquid solution of indole-butyric acid (IBA) in the concentrations of 0, 3000 and 6000 mg L-1 and then were placed in tubes containing commercial substrate. The experimental design was completely randomized with factorial 2 x 3 x 6 (mini-cutting length x IBA concentration x time of collection), with four replications, it being each plot varied according to the amount of shoots obtained by period time. After 120 days, the rooting and callus formation (%), average number of roots per mini-cutting and the average length of the roots were evaluated. After 60 days of these evaluations, the survival of mini-cuttings rooted after transplant was evaluated. It was evaluated also the production of mini-cuttings of each size in each period time. At the end of the experiment it was evaluated the percentage of survival of mother plantlets. For analysis of tryptophan was used materials branches, leaves and twigs with leaves, taken from the materials used for the production of mini-cutting. It was recommended for hybrid jabuticaba tree the use mini-cutting with eight cm, treated with 6000 mg L-1 of IBA and collected in June. For jabuticaba tree of cabinho and araça amarelo tree the period for propagation by mini-cuttings should be in August, regardless of IBA concentration and length of the mini-cutting. In the jabuticaba tree sabara and sete capote tree is important to obtain more satisfactory results realized the collect in October or December, with the same independence of other levels tested in other factors. However, for sete capote tree should test other techniques to increase the efficiency of propagation. And with pitanga tree recommended to the collection in June, but with 6cm the application of 3000 mg L-1 of IBA and 8 cm with 6000 mg L-1 of IBA.