Made available in DSpace on 2015-03-26T13:58:37Z (GMT). No. of bitstreams: 1 texto completo.pdf: 904859 bytes, checksum: 818ef8bd958bac77d3e6400dba52155c (MD5) Previous issue date: 2013-09-19 / Brazil is among the greatest world producers and traders of agricultural products. The inevitable growth of international trade, combined with the country s huge borders contributes, nevertheless, to increased risk of entrance and establishment of pests that may cause significant economic, social and environmental impact. The Ministry of Agriculture, Livestock and Food Supply s (MAPA) decentralized plant protection units, although operating in line with legal regulation and guidelines, and within the limits of its regimental competencies, do not have a procedural manual. The objective of this dissertation is to develop a procedural manual to guide the performance of the plant protection activities. The methodology used in planning, research, mapping and construction of the manual is the one developed and adopted by MAPA s Planning and Management Coordination, through its Mapping, Improvement and Implementation of Service Processes Manual (MAIPS). As a result of field research, twenty-four sub-processes have been mapped, comprised in three service processes that compose the macro-process Plant Health of MAPA s decentralized plant protection units. In general, research demonstrated that there are no written standard operational procedures in place and that the forms are not standardized. Throughout the improvement stage, a Procedural Manual has been built, composed by flowcharts, activity descriptions, tasks, rules and forms. / O Brasil ocupa posição de destaque no mundo como produtor e exportador de produtos agropecuários. O incremento do comércio internacional, aliado às dimensões do país, contribui, entretanto, para maior risco da entrada e estabelecimento de pragas que podem causar significativo impacto econômico, social e ambiental. As unidades de sanidade vegetal das Superintendências Federais de Agricultura, Pecuária e Abastecimento (SFAs), embora atuem embasadas nas normas legais e diretrizes emanadas pelo Departamento de Sanidade Vegetal da Secretaria de Defesa Agropecuária do Ministério da Agricultura, Pecuária e Abastecimento (DSV/SDA/MAPA) e no escopo de suas competências regimentais, não dispõem de um manual de procedimentos e de formulários que estabeleça padrões para execução de suas atividades. Esta dissertação teve por objetivo desenvolver um manual de procedimentos para a realização dessas atividades. A metodologia empregada no planejamento, pesquisa, mapeamento e elaboração do manual foi aquela desenvolvida e adotada pela Coordenação de Planejamento e Gestão do MAPA, por meio de seu Manual de Mapeamento, Aprimoramento e Implementação de Processo de Serviço (MAIPS). Como resultado da pesquisa de campo, foram mapeados vinte e quatro subprocessos, compreendidos em três processos de serviço que compõem o macroprocesso Sanidade Vegetal das SFAs. Verificou-se ainda que, regra geral, não existem procedimentos operacionais padrão escritos e que os formulários utilizados não são uniformizados. Como resultado da fase de aprimoramento, foi construído o Manual de Procedimentos para as Unidades de Sanidade Vegetal das SFAs, composto de fluxogramas, descrições de atividades, tarefas, regras e formulários.

Made available in DSpace on 2015-03-26T13:58:39Z (GMT). No. of bitstreams: 1 texto completo.pdf: 1156754 bytes, checksum: ec0b59041b7c6272f3daf112a5c980d1 (MD5) Previous issue date: 2013-12-12 / In Brazil, the control of the boll weevil population has been highlighted by the consolidation of a public-private partnership, through the State Agencies for Animal and Plant Production and Health, Ministry of Agriculture, EMBRAPA, State Foundations, Growers Associations, and State Funds of Incentives for Cotton Production, that coordinate and promote the Programs of Prevention, Control and of Suppression of the Cotton Boll Weevil. These efforts have shown satisfactory results in suppressing the pest over the years, sharply reducing yield losses and reducing insecticide applications. Based on the results of research and experience with the legislative control for implementation of the Programs in the State of Goias, in this study is proposed a legislation to meet the interests of cotton growers that adopt the methodologies of the weevil population suppression. The legislation proposed is based on the guidelines of the International Standards for Phytosanitary Measures - ISPM for determining a Low Prevalence Area of Pest - LPAP for the Boll Weevil - Anthonomus grandis, which will subsidize a government policy aimed at the phytossanitary control of the weevil. / No Brasil, o controle populacional do bicudo-do-algodoeiro vem se destacando pela consolidação de uma parceria pública e privada, através dos Órgãos Estaduais de Defesa Agropecuária, do Ministério da Agricultura, da EMBRAPA, das Fundações Estaduais, das Associações de Produtores e Fundos de Incentivos Estaduais à Cotonicultura, que coordenam e fomentam os Programas de Prevenção, Controle e de Supressão do Bicudo-do-Algodoeiro. Ao longo dos anos essas ações demonstraram resultados satisfatórios na supressão da praga, reduzindo acentuadamente as perdas provocadas e diminuindo as aplicações de inseticidas. Baseando-se nos resultados de pesquisas e na experiência obtida no acompanhamento da fiscalização do controle legislativo para implantação dos Programas de Controle e Supressão no Estado de Goiás, é proposto no presente estudo uma legislação para atender ao interesse dos produtores que realizam as metodologias de supressão populacional do bicudo. A minuta de legislação é baseada nas diretrizes das Normas Internacionais de Medidas Fitossanitárias NIMF, para a determinação de uma Área de Baixa Prevalência da Praga ABPP para o Bicudo-do-Algodoeiro Anthonomus grandis, a qual subsidiará uma política governamental voltada para o controle fitossanitário da praga.

Made available in DSpace on 2015-04-20T12:22:46Z (GMT). No. of bitstreams: 1 TESE CRISTOVAO.pdf: 2860574 bytes, checksum: 30d47db4b1a98ddd577508efff6caad6 (MD5) Previous issue date: 2011-06-04 / Fundação de Amparo à Pesquisa do Estado do Amazonas / The guarana cultivation has great social and economic importance in Maués City. Aimed at increasing crop yields, studies are needed on the plant population per unit area. This work aimed to evaluate the effects of different plant stands in the cultivation of guarana on aspects of vegetative growth, diseases, soil fertility and productivity, to improve the productive use of areas with the culture of guarana and reducing the impact of the implementation of the culture in new areas, providing an economic return for producers of guarana in Maués City, Amazonas State. We studied two clones BRS-Amazonas (CMU-300) and BRS-Maués (CMU-871) in booths of 625, 833, 1,111, 1,666, 2,500 and 5,000 plants per hectare. The experimental design for the analysis was completely randomized in factorial arrangement 2x6 and the number of replicates ranged from 3 to 10 according to the variables studied. The increase in the plant stands showed the economic viability for all indicators, with low risk of obtaining unfavorable results in the region analyzed when the used clones show high resistance to major diseases affecting the crop. There is need for continued evaluation to define the better stand of plants per unit area, which should be set at the end of at least five years of evaluation, after the stabilization of yield per plant. / O cultivo do guaranazeiro tem grande importância social e econômica na região do município de Maués. Visando o aumento da produtividade da lavoura, são necessários estudos sobre a população de plantas por unidade de área cultivada. Este trabalho teve como objetivo avaliar os efeitos de diferentes estandes de plantas no cultivo do guaranazeiro sobre aspectos de crescimento vegetativo, doenças, produtividade e fertilidade do solo, possibilitando uma melhor utilização das áreas utilizadas com a cultura do guaranazeiro, reduzir o impacto da implantação da cultura em novas áreas apresentando um retorno econômico aos produtores de guaraná do município de Maués, Estado do Amazonas. Foram estudados duas cultivares BRS-Amazonas e BRS-Maués em estandes de. 625, 833, 1.111, 1.666, 2.500 e 5.000 plantas por hectare. O delineamento experimental adotado para a análise foi o inteiramente casualizado em arranjo fatorial 2 x 6, sendo que o número de repetições variou de 3 a 10, de acordo com as variáveis estudadas. O aumento do estande de plantas mostrou viabilidade econômico-financeira para todos os indicadores, com baixo risco de obter resultados desfavoráveis na região analisada quando utilizadas cultivares que apresentem alta resistência às principais doenças que afetam a cultura. Existe a necessidade de se definir o estande ideal de plantas por unidade de área, considerando o tempo de estabilização da produção que ocorre a partir do quinto ano.

Ácaros fitoseídeos são eficientes predadores de ácaros pragas em algumas culturas. A precisa identificação das espécies é o passo inicial na seleção de inimigos naturais em um projeto de controle biológico. Os ácaros são geralmente identificados com base nas características morfológicas, mas aspectos biológicos e ecológicos, e mais recentemente características moleculares vêm sendo usadas nesse processo. Populações dos fitoseídeos identificados como Euseius citrifolius Denmark & Muma provenientes de Arroio do Meio-RS, Campinas-SP e Petrolina-PE, e Euseius concordis (Chant) procedentes de Arroio do Meio, Jaguariúna-SP, Petrolina, Pontes e Lacerda-MT e Viçosa-MG foram estudados em relação a morfologia, compatibilidade reprodutiva e características moleculares. A caracterização morfológica correspondeu as medições de estruturas de fêmeas e machos. A compatibilidade reprodutiva foi avaliada através de cruzamentos e retrocruzamentos homogâmicos e heterogâmicos. A caracterização molecular foi realizada com o seqüenciamento dos espaços internos transcritos (ITS1 e ITS2) do DNA ribossomal. Relações significativas foram observadas dentro de cada população entre o comprimento médio das setas e as respectivas amplitudes de variação. Ambos os sexos de E. citrifolius de Petrolina e E.concordis de Jaguariúna tiveram algumas setas mais curtas que as demais populações da mesma espécie; esta última diferiu marcadamente da população de Petrolina. A comparação das medições das estruturas de cada população e dos espécimes tipo de E. citrifolius e E. concordis confirmaram a identificação morfológica preliminar das populações ao nível de espécie. Medições de machos resultantes de cruzamentos heterogâmicos indicaram que essas espécies se reproduzem por pseudo-arrenotoquia. Incompatibilidade parcial foi observada nos cruzamentos heterogâmicos envolvendo fêmeas de E. citrifolius de Petrolina; descendentes produziram poucos ovos e inviáveis quando retrocruzadas com machos das populações parentais. Machos de Petrolina produziram descendentes viáveis quando cruzados com fêmeas de Arroio do Meio e Campinas. Não ocorreu oviposição nos cruzamentos e retrocruzamentos heterogâmicos envolvendo fêmeas de E. concordis de Petrolina. Nos cruzamentos heterogâmicos envolvendo machos de Petrolina a oviposição foi reduzida e somente machos (viáveis) foram produzidos. Cruzamentos de fêmeas de Pontes e Lacerda e machos de Jaguariúna e vice-versa produziram somente machos. Entretanto o fluxo gênico entre essas populações seria possível indiretamente, através de cruzamentos entre essas populações e a população de Arroio do Meio. Populações de Arroio do Meio, Jaguariúna, Pontes e Lacerda e Viçosa pertencem a mesma espécie. Maior variação entre as populações foi observada no espaçador ITS1. O seqüenciamento dos espaçadores ITS1 e ITS2 permitiu discriminar entre os grupos de populações identificadas como E. citrifolius de E. concordis. O seqüenciamento dos ITSs pode ser aplicado como uma ferramenta complementar na identificação de fitoseídeos. Apesar do fato de que alguns tipos de diferenças foram sempre observadas nesta tese entre a população de Petrolina identificada preliminarmente como E. concordis, não é conveniente descrever esta população como uma nova espécie, devido a dificuldade em separar indivíduos dessa população daqueles das populações identificadas como E.concordis. Para uma conclusão, é sugerido que outros estudos de cruzamentos sejam conduzidos com populações morfologicamente identificadas como E. concordis coletadas entre Petrolina e Viçosa, e que a caracterização molecular envolvendo o gene citocromo oxidase seja conduzida. / Phytoseiidae mites are efficient predators of pest mites on several crops. Precise identification is the initial step in the selection of natural enemies in a biological control project. Mites are usually identified by their morphology, but biological and ecological aspects and, more recently, molecular characteristics have also been used in this process. Populations of phytoseiid mites identified as Euseius citrifolius Denmark & Muma from Arroio do Meio-RS, Campinas-SP and Petrolina-PE, and E. concordis (Chant) from Arroio do Meio, Jaguariúna-SP, Petrolina Pontes e Lacerda-MT and Viçosa-MG were studied in relation to morphology, reproductive compatibility and molecular characteristics. Morphological characterization corresponded to measurements of structures of females and males. Reproductive compatibility was evaluated by homogamic and heterogamic crosses and backcrosses. Molecular characterization was done by sequencing the internal transcribed spacers of the ribosomal DNA (ITS1 and ITS2). Significant relationships were observed within each population between mean setal lengths and the respective ranges. Both sexes of E. citrifolius from Petrolina and E. concordis from Jaguariúna had some setae shorter than other populations of the same species; the latter differed most markedly from the Petrolina population. A comparison of the measurements of structures for each population and type specimens of E. citrifolius and E. concordis confirmed the preliminary morphological identification of the populations at species level. Measurements of males resulting from heterogamic crosses indicated that both species reproduce by pseudo-arrhenotoky. Partial incompatibility was observed in heterogamic crosses involving females of E. citrifolius from Petrolina; progeny produced just few, unviable eggs when backcrossed with males of the parental populations. Males from Petrolina produced viable offspring when crossed with females from Arroio do Meio or Campinas. No eggs were produced in heterogamic crosses and backcrosses involving females of E. concordis from Petrolina. In heterogamic crosses involving males from Petrolina, oviposition was reduced and only (viable) males were produced. Crosses of females from Pontes e Lacerda and males from Jaguariúna and vice-versa produced only male progeny. However, gene flow between those population could be possible indirectly, through crosses between those populations and Arroio do Meio population. Populations from Arroio do Meio, Jaguariúna, Pontes e Lacerda and Viçosa belong to a same species. Most of the molecular variation between populations was observed in ITS1. The sequencing of ITS1 and ITS2 allowed the discrimination between the group of populations identified as E. citrifolius from that identified as E. concordis. With the information presently available, sequencing of ITSs can be applied as a complementary tool to identify phytoseiids. Despite the fact that some type of differences were always observed in this thesis between the Petrolina population preliminarily identified as E. concordis and the remaining populations, it is not convenient to describe such population as a new species presently, given the difficulty in separating individuals from that population from those of populations identified as E. concordis. For a conclusion, it is suggested that other crossing studies be conducted with populations morphologically identifiable as E. concordis collected between Petrolina and Viçosa, and that the characterization of the cytochrome oxidase gene be conducted.

A Mata Atlântica (MA) é um dos ambientes naturais mais ameaçados do Brasil, tendo sua área original atualmente bastante reduzida pelas ações antrópicas. As atividades agrícolas estão incluídas entre os fatores de impacto a este Bioma; e inserida na área de domínio da MA está a região de Apiaí - SP, produtora de tomate (Solanum lycopersicum L.) de mesa, uma cultura caracterizada pela sua importância alimentar, e responsável por significativos impactos socioambientais devido ao uso de agroquímicos, desmatamentos e más condições de trabalho. Devido à infestação por insetos-praga e às ocorrências de doenças agrícolas, esta cultura é considerada uma das mais exigentes em tratos culturais necessitando pulverizações constantes e uso geral de agroquímicos. Este trabalho busca ampliar o conhecimento sobre as funções que a biodiversidade florestal pode ter como um instrumento para auxiliar o manejo agrícola de insetos fitófagos e doenças em cultivos de tomate de mesa. No município de Apiai - SP, entre 2008 e 2010, foram instalados 5 módulos experimentais de cultivo de tomate em manejo agroecológico no modelo de Ilhas de Alta Produtividade (IAPs), em locais onde a biodiversidade florestal é um elemento presente no entorno. Em cada módulo de cultivo, foram analisados: a biodiversidade florestal no entorno até uma distância de 300 m nos sentidos N, S, L, O; a ocorrência de insetos-praga e doenças agrícolas e a viabilidade econômica e socioambiental. Os resultados foram comparados aos mesmos dados coletados de outros 5 cultivos convencionais da mesma região ecológica, que cultivaram variedades semelhantes, no mesmo período, em ambientes com menor biodiversidade florestal nativa em seu entorno. Os módulos experimentais de cultivo agroecológico demonstraram uma área media de cobertura Florestal Atlântica em torno de 43,82%%, com uma ocorrência media de 46 espécies arbóreas nativas com Índice de Biodiversidade Florestal de 3,87; os cultivos comparativos apresentaram áreas médias de cobertura florestal em seu entorno de 31,34 %, com uma ocorrência de 26 espécies arbóreas nativas e índice de biodiversidade florestal de 3,44. Em amostras de 20 plantas de tomate por cultivo, nas áreas experimentais, não foram identificadas a ocorrência de viroses, uma única espécies causadora de doença fúngica e um total de 4 espécies de insetos-praga foram identificadas. Os produtores convencionais comparados tiveram a ocorrência de 11 espécies de doenças fúngicas e bacterianas, com 7% de ocorrência de viroses e 7 espécies de insetos fitófagos. Os dados mostraram que existe relação da biodiversidade florestal com o número de espécies de insetos-praga nos cultivos. Em uma análise de viabilidade socioambiental entre os cultivos experimentais e comparativos, foi identificada uma maior taxa de risco, investimento, produtividade, volume de produção, e prejuízos financeiros, bem como maiores impactos ambientais e sociais entre os cultivos de tomate convencionais comparativos. Os modelos experimentais com biodiversidade florestal em seu entorno se mostraram viáveis social e ambientalmente para utilização prática na agricultura familiar na região de Apiai - SP. / The neotropical Atlantic Rain Forest (MA) is one of the most threatened natural environments of Brazil, with its original area now greatly reduced by human actions. Agricultural activities are included among the impact factors in this biome, and inserted in the area with the MA is the region of Apiaí - SP producer of tomato (Solanum lycopersicum L.) table, a culture characterized by its nutritional importance and also responsible for significant environmental impacts due to the use of agrochemicals, deforestation and poor working conditions. Due to infestation by insect pests and plant diseases this crop is considered one of the most demanding in cultivation requirements such as constant spraying and general use of agrochemicals. This work seeks to expand the knowledge about the functions that forest biodiversity can perform as a tool to assist the cultivation control in the control of phytophagous insects and diseases in tomato crop. In the district of Apiaí - SP, from 2008 to 2010, five modules have been installed for experimental cultivation of tomato in the agroecologic model called Islands of High Productivity (IAPs), in places where forest biodiversity is an element current around. The biodiversity in the surrounding forest to a distance of 300 m in the directions N, S, E, W, the occurrence of insect pests and diseases and also the economic viability and environmental impact were analyzed in each module of cultivation. The results were compared to the same data collected from five other conventional crops in the same ecological region which cultivated similar varieties in the same period in environments with less native forest biodiversity in their environment. The experimental agroecologic modules showed an average area of Atlantic Forest cover around 43.82%, with an average occurrence of 46 native tree species and forest biodiversity indices of 3.87; the conventional modules had 31.34% of atlantic forest cover, with an occurrence of 26 native tree species and forest biodiversity indices of 3.44. In the experimental areas, with sampling of 20 tomato plants/ cultivation, were not identified any occurrence of viruses but only a single species causing fungal disease and a total of four species of insect pests. The conventional producers had the occurrence of 11 species of fungal and bacterial diseases, with 7% occurrence of viruses and 7 species of phytophagous insects. The data showed that there are relationship of forest biodiversity and number of species of insect pests in crops. In analysis of economic and socio-environmental viabilities between the experimental and comparative cultivations, it was identified a higher rate of risk, investment, productivity, production volume and financial losses, as well as higher environmental and social impacts of the conventional tomato cultivations. The agroecologic experimental models with higher forest biodiversity around have proved being socially and environmentally viable for practical use in family farming in the region of Apiai - SP.

Plant health system is an important element for the control and management of plant pests to ensure food production and security as well as safe trade. The objectives of this study were to: identify the existing knowledge gaps on current plant health system at the national and provincial level, identify critical areas to be addressed in the current plant health institutional framework and identify the existing limitations and risks on current legislation. A semi-structured questionnaire was used to collect the data. The data was analysed statistically using the one-way frequency and Spearman’s Rank correlation coefficients.The study found that the Provincial Departments of Agriculture (PDAs) (below 50%) lack knowledge of plant health system as compared to the national authorities: DAFF and DEA (above 70%). The study also found that there were no formal linkages between the national and provincial authorities. The study further revealed that there is a need for harmonisation of relevant legislation. It can be concluded that there are knowledge gaps on plant health matters between the national authority and the provincial level and that there are no formal linkages within the spheres of government on plant health matters. / Agriculture, Animal Health and Human Ecology / M. Sc. (Agriculture)

This study aimed to develop a novel end-to-end plant diagnosis model for the analysis of plant health conditions in near real-time to optimize the rate of production on farmlands for an intensive, yet environmentally safe farming production to preserve the natural environment. First, field research was conducted to determine the extent of the problems faced by farmers in agricultural production. This allowed us to refine the research statement and the level of technology involved in the production processes. The advantages of unmanned aerial systems were exploited in the continuous monitoring of farm plantations to develop automated and accurate measures of farm conditions. To this end, this thesis applies the Precision Agricultural technology as a data based management system that takes into account spatial variations by using the Global Positioning System, Geographical Information System, remote sensing, yield monitors, mapping, and guidance system for variable rate applications. An unmanned aerial vehicle embedded with an optic and radiometric sensor was used to obtain high spectral resolution images of plantation status during normal production/growth cycle. Then, an ensemble of classifiers with Convolution Neural Networks (CNN) was used as off the shelf feature extractor to train images to develop an end-to-end feature detection and multiclass classification system for plant overall health’s conditions. Whereby previous works have concentrated on using CNN as off the shelf feature extractor and model training to detect only plant diseases from plants. To date, no research has yet been carried out to develop an end-to-end model for the overall plant diagnosis system. Previous studies focused on the detection of diseases at any given time, making it difficult to implement comprehensive real-time PA systems. Applying the pretrained model to the new images showed that the model can accurately predict any plant condition with an average of 97% accuracy.

La banane plantain (Musa paradisiaca) est l’aliment de base de millions de personnes dans le monde et sa culture génère des revenus permanents pour un grand nombre d’agriculteurs, dans des plantations de taille petite ou moyenne. Comme dans d’autres régions tropicales, la culture du plantain en Guadeloupe et en Haïti est soumise à de fortes contraintes parasitaires aussi bien telluriques (i.e. du sol : nématodes phytoparasites et charançon du bananier) qu’aériennes (Cercosporiose noire notamment). Les moyens de lutte conventionnels reposent sur l’usage de produits de synthèse dont les effets néfastes sur l’environnement (sols, eaux, animaux) comme sur la santé humaine ne sont plus supportables. Il est donc urgent de réfléchir à des solutions agro-écologiques permettant de rétablir les équilibres biologiques, de maintenir une bonne qualité des sols et une production optimale dans les systèmes de culture plantains. C’est le but de ce travail de thèse qui couple la réalisation d’un diagnostic agro-écologique dans des parcelles paysannes, et le test d’alternatives agro-écologiques en milieu semi-contrôlé. Pour ce faire, une typologie des systèmes de culture plantains a été réalisée à l’issue d’une enquête agro-environnementale dans les deux zones d’étude. Elle a permis de sélectionner 23 parcelles en Guadeloupe et 12 en Haïti dans lesquelles un diagnostic agro-écologique a été conduit. Sur la base de ce diagnostic et de la recherche d’alternatives agro-écologiques à l’usage des produits chimiques, une expérimentation au champ a été mise en place en Guadeloupe en station de recherche, permettant le test de trois pratiques culturales innovantes pour le plantain (seules et combinées), à savoir : i) l’introduction d’une plante de service Paspalum notatum pour la gestion des adventices et la réduction de l’utilisation d’herbicides ; ii) l’apport de vermicompost pour le contrôle des nématodes phytoparasites inféodés au bananier plantain et la fertilisation de celui-ci ; iii) l’utilisation de plants sains PIF (Plants Issus de Fragments de tiges) indemnes de nématodes et de larves de charançon du bananier. La typologie des systèmes de culture plantains révèle que : i) en Guadeloupe les précédents : jachère, ananas et banane plantain sont prédominants avec un niveau d’intensification faible (apports d’intrants chimiques faibles et peu fréquents) ou élevé (apports d’intrants chimiques élevés et plus fréquents) ; ii) en Haïti, les précédents : jachère, banane plantain et manioc prédominent avec un niveau d’intensification faible ou nul (apport d’intrants inexistant). Les résultats du diagnostic agro-écologique montrent que, i) lorsque le niveau d’intensification est faible, les bananeraies plantains pérennes et le précédent-ananas permettent de maintenir une bonne qualité du sol et une bonne régulation des parasites telluriques ; ii) lorsque le niveau d’intensification est fort, les populations d’ingénieurs du sol diminuent drastiquement, alors que le cortège parasitaire tellurique augmente sans que cela n’affecte l’obtention de bons niveaux de rendement instantannés (parcelles précédées d’ananas ou d’une jachère principalement) ; iii) en absence totale de fertilisation, il résulte une diminution de l’activité biologique du sol mais aussi du rendement du plantain, exacerbé par le choix des précédents-culturaux (manioc ou banane plantain) en lien avec les contraintes pédoclimatiques et la maladie des raies noires (Cercosporiose noire) causée par Mycosphaerella fijiensis, notamment au sein des parcelles Haïtiennes ; iv) la succession plantain/plantain est la plus pénalisante vis-à-vis de la culture du plantain, car quelque soit le niveau d’intensification, le rendement reste relativement faible, en lien avec une dégradation de l’état sanitaire, comparativement aux autres précédents. / Plantain (Musa paradisiaca) is the staple food of millions of people worldwide and its cropping generates ongoing revenues for many farmers who are planting small or medium size areas. As in other tropical regions, plantain cultivation in Guadeloupe and Haiti is under heavy parasitic constraints terrestrial (plant-parasitic nematodes and banana weevil) as well as aerial (black Sigatoka in particular). Conventional means of control based on the use of synthetic products which adverse effects on the environment (soil, water, animals ...) as on human health are not bearable any more. It is thus urgent to think about agroecological solutions allowing to restore the biological balances, to maintain good soil quality and optimal plantain cropping systems.This is the ultimate goal of this thesis which couples the realization of an agroecological diagnosis in peasants’ plots, and the test of agroecological alternatives in semi-controlled conditions. To do this, a typology of plantain cropping systems was carried out from an agrienvironmental survey in the two study areas. It allowed to select 23 plots in Guadeloupe and 12 in Haiti in which an agroecological diagnosis was implemented. Based on this diagnosis and the research of agroecological alternatives to the use of chemicals, a field experiment was set up in Guadeloupe, in an experimental station allowing the test of three innovative practices for plantain cultivation (alone and combined), namely : i) the introduction of a cover-crop Paspalum notatum for weed control while reducing the use of herbicides ; ii) the input of worms’ compost to control plant-parasitic nematodes specific to plantain and to fertilize it ; iii) the use of healthy “PIF” plants (plants issued from stem fragments) free from telluric pests (nematodes and weevil’s larvaes).The typology of plantains cropping systems shows: i) in Guadeloupe the previous crops are: fallow pineapple and plantain predominate with a low level of intensification (low and infrequent chemical inputs) or high (high and frequent chemical inputs); ii) in Haiti, the previous crops are: fallow, plantain and cassava predominate with a low level or no intensification at all (no inputs). The results of the agroecological analysis show that : i) when the level of intensification is low, perennial plantain and pineapple as previous crops help maintaining a good soil quality and a good regulation of the telluric pests ; ii) when the level of intensification is strong, the soil engineers drastically reduce, while the density of telluric parasites increases without affecting good levels of instantaneous yields (plots where the previous crop is pineapple or mostly fallow) ; iii) when the fertilization is totally missing, it decreases the biological activity of the soil furthermore the plantain yields, exacerbated by the choice of the previous crop (cassava or plantain), in connection with soils and climate constraints and the black Sigatoka caused by Mycosphaerella fijiensis, especially in the Haitian plots ; iv) the crop succession plantain/plantain is the most critical regarding the plantain’s cropping, because whatever the level of intensification, the yields remain relatively low in connection with a degradation of the health state, compared to other previous crops.The driving of an experiment in a research station shows that on the scale of one year, the three tested innovative practices allow maintaining a good soils quality. Healthy plants "PIF" have a better health state (absence of plant parasitic nematodes in the roots) which helps a significant increase of the yields. Cover-crop P. notatum helps the weeds and the soil pests control and favors the improvement of soil biological activity and plantain yields. Worms’ compost contributes to the maintenance of a better soils quality while allowing the regulation of the populations of plant-parasitic nematodes of the plantains.