This thesis is focused on poultry meat and its products. A definition of meat, sources of meat, types of poultry production and own goal of the thesis are explained in the first chapter. Then there are described technological processes of poultry treatment in common with a veterinary and hygienic supervision which provides a check of the production and processing of poultry meat. In addition, there are mentioned some manufacturing companies which are engaged in poultry processing. The third and the fourth chapters are about a general characteristic and a quality of poultry meat. There are described issues related with poultry meat and its quality, structure, production and consumption in the following chapter. The last chapter is theoretical and there is taking apart the main problem of this thesis which means the production of poultry meat products, other poultry products and an overview of legislation related to poultry meat products. The practical part is focused on consumer attitude to poultry meat and poultry meat products and other kinds of meat by a questionnaire. A discussion and evaluation are based on resulting graphs. An appropriate conclusion was developed from this part of thesis.

Os consumidores estão cada vez mais conscientes e informados sobre a relação entre ingestão de sódio e o desenvolvimento da hipertensão. Desta forma, a indústria de alimentos vem desenvolvendo numerosos produtos com baixo teor de sódio para atender às necessidades deste novo perfil de consumidores. O presente projeto estudou os impactos tecnológicos, sensoriais e microbiológicos em salsichas com teor reduzido de sódio, adicionadas de fosfato e cloreto de potássio. Foram realizadas avaliações em duas matrizes cárneas: a) com carne bovina e suína e b) de carne mecanicamente separada (CMS) de frango. Na primeira etapa foram estudadas emulsões, na qual a otimização do processo utilizou como técnica o planejamento fatorial com delineamento composto central rotacional - DCCR, baseado na Metodologia de Superfície de Resposta, sendo as variáveis independentes: fosfato, cloreto de sódio e cloreto de potássio. Esta etapa foi realizada em sistema modelo obtendo as massas em mini cutter com todos os ingredientes previstos para obtenção de salsicha, variando os teores de fosfato, sal (NaCl) e cloreto de potássio (KCl). Na segunda etapa os melhores níveis de fosfato e KCl foram fixados e o cloreto de sódio continuou sendo avaliado em 3 níveis (1,00%, 1,30% e 1,75%), os tratamentos foram processados em planta piloto e realizados as análises físico-químicas, tecnológicas, microbiológicas e sensoriais. Os tratamentos foram monitorados ao longo de 56 dias nos produtos embalados à vácuo e estocados à 5 ± 2ºC em cinco períodos (1, 14, 28, 42 e 56 dias). Na emulsão de carne os melhores níveis de KCl e fosfato foram 0,85% e 0,25% respectivamente e na salsicha os tratamentos com 1,30 e 1,75% de NaCl apresentaram melhor desempenho na maioria das análises, principalmente na análise sensorial com consumidor. Já na emulsão de CMS os melhores níveis de KCl e fosfato foram 0,46% e 0,40%, respectivamente e na salsicha de CMS os tratamentos com 1,3% e 1,75% de NaCl apresentaram os melhores resultados principalmente na análise sensorial com consumidor. A redução da adição de 1,75% de NaCl para 1,3%, representa uma redução de cloreto de sódio de aproximadamente 25% e é viável do ponto de vista tecnológico, microbiológico e sensorial. / Consumers are increasingly aware and informed about the relationship between sodium intake and the development of hypertension with subsequent coronary problems. Thus, the food industry has developed numerous products with low sodium content to meet the needs of this new consumer profile. This project studied the technological, sensory, and microbiological impacts on low sodium sausages, with phosphate and potassium chloride added. Evaluations were performed in two meat matrices: a) beef and pork b) mechanically recovered poultry meat (MRPM). In the first stage, emulsions were studied, in which the optimization process used the technique of factorial design with a central composite design, based on Response Surface Methodology, and the independent variables were phosphate, sodium chloride and potassium chloride. This stage was performed in a model system obtaining the mass in a mini cutter with all the ingredients to obtain sausage with varying levels of phosphate, salt (NaCl) and potassium chloride (KCl). In the second stage, the best levels of phosphate and KCl were fixed and sodium chloride remained rated at three contents (1.00 %, 1.30 %, and 1.75 %). The treatments were processed in a pilot plant and the physical/chemical, technological, microbiological, and sensorial analyses were performed. The treatments were monitored over 56 days in vacuum packaged products and stored at 5 ± 2°C in five periods (1, 14, 28, 42, and 56 days). In meat emulsion, the best contents of KCl and phosphate were 0.85% and 0.25%, respectively. In sausage, the treatments with 1.30 and 1.75% NaCl showed the best performance in most analyses, mainly in the sensory analysis. In emulsion of mechanically recovered poultry meat, the best contents of KCl and phosphate were 0.46 % and 0.40 %, respectively, and sausage MRPM treatments with 1.3% and 1.75% NaCl showed the best results mainly in the sensory analysis. The reduction of 1.75% to 1.3% of NaCl represents a reduction of about 25% of sodium chloride and is viable in terms of technological, microbiological, and sensorial aspects.

<p>It is a well-known fact that there are significant differences in production output and business results of farms operating under rather similar natural and socio-economic conditions. Such differences can be attributed to biological factors, specialization level and production intensity, size of farms, implemented production practice, but also to the differences in capabilities of farmers, that is, their managerial capacities.<br />The starting point in selection of subjects for the research was significance of managerial capacities and the fact that not enough attention is dedicated to them, as well as the necessity of urgent improvement of competitiveness in production of poultry meat in our country. Therefore, the subject of the research in this doctoral dissertation is managerial capacities of broiler producers in Vojvodina. When analysing managerial capacities of broiler producers, not only were personal aspects taken into account (gender, age, education level, previous experience, etc.), but the subject of consideration includes characteristics of decision making process (planning, implementation and control) as well. The main objective of the study is to point out to the significance of managerial capacities, possibilities and methods implemented in their assessment, and to establish the reason why some of the broiler producers in Vojvodina, operating under rather similar conditions, are more efficient as compared to other farmers. In order to analyse managerial capacities of broiler producers and to judge whether and to what extent do they influence the production output (technical and economic efficiency), 30 broiler farms of different production capacities, all at the territory of AP Vojvodina, were comprised in the analysis.<br />One of the starting hypotheses in this study considers significant differences in technical and economic efficiency between the selected broiler farms. Technical and economy efficiency indexes, obtained by implemented DEA method, indicate that such hypothesis was justified. The obtained TE indexes point out that broiler producers in Vojvodina are relatively technically efficient (six out of thirty producers achieved maximum level of TE). However, the number of farms that achieved maximum level of EE is much lower (only one out of thirty farms achieves maximum level of EE), which indicates the market difficulties that producers are coping with, but also proves that it is possible to operate better under the existing conditions which are assumed to be the same for all. The number of economically inefficient farms could be reduced through decrease of production costs (primarily reduction of feed costs, increase of operation productivity, reduction of energy costs, etc.), which certainly requires, as one of preconditions, the adequate managerial capacities of producers.<br />The assumption that certain aspects of managerial capacities, such as personal aspects and decision making process, influence TE and EE has been confirmed with the results of Tobit regression. Statistically significant influence of achieved TE and EE is confirmed in 9 out of 82 variables included in the models. This certainly does not mean<br />VIII<br />that other aspects of managerial capacities are not significant, but that under current conditions, relatively small number of variables determines the number of farms that will manage to achieve or at least to nearly achieve &ldquo;the best possible practice&rdquo;.<br />Statistically significant influence was also proven in certain personal aspects of producers, as well as in some aspects of decision making process. Therefore, it is necessary to explain to producers and advisors the significance of the aforesaid aspects and to work on their improvement. The only aspect proven as significant both for TE and EE is the experience of farmers, which only explains the poor working environment that our producers operate in. The conclusion which can be derived from the aforesaid is that the most significant and irreplaceable managerial capacity of broiler producers is their experience. Such experience mainly refers to business relations and acquaintances obtained from long-lasting engagement in broiler production, but also to their ability to find their way and achieve success in conditions of the black economy.<br />The fact that there is no statistical significance in terms of influence of certain aspects of managerial capacities on TE and EE, actually points out to underdevelopment of the required managerial capacities of broiler producers. Therefore, the improvement of managerial abilities must be set as the permanent task, so competitiveness of poultry meat sector could be increased. To this effect, it is necessary to provide constant education through trainings and seminars, as well as regular cooperation with advisory service (particularly in terms of significance of keeping records, planning and control of the achieved results), but also through exchange of experience and cooperation with other producers.</p>

In this project the factors influencing the stability and antimicrobial activity of allyl isothiocyanate (AITC) against Campylobacter jejuni, Salmonella or Listeria monocytogenes as well as factors that enhance sinigrin (glucosinolate in Oriental mustard) hydrolysis by these pathogens were investigated. The minimum inhibitory concentration (MIC) of AITC against 5 strains of each of Salmonella or L. monocytogenes, ranged from 60-100 ppm at 37 ºC. This was reduced to 10-40 ppm at 21 ºC and a further reduction to 5-10 ppm against strains of L. monocytogenes was observed at 4 ºC. This was attributed to greater stability of AITC as temperature was decreased. C. jejuni strains were more susceptible to AITC with MICs of 0.63-1.25 ppm and 2.5-5 ppm at 37 and 42 ºC, respectively. AITC was more inhibitory at ≤ 21 ºC against Salmonella with acidic pH or against L. monocytogenes with neutral pH. C. jejuni, Salmonella and L. monocytogenes strains and mixtures had the ability to degrade sinigrin to form inhibitory concentrations of AITC, and sinigrin hydrolysis was significantly enhanced by higher incubation temperature (21 ºC > 10 ºC > 4 ºC), the presence of 10 mM ferric or ferrous irons, and the presence of < 0.25% glucose. This project also investigated the antimicrobial activity of AITC or Oriental mustard extract alone or combined with ethylenediamine tetraacetic acid (EDTA), malic acid and acetic acid in edible antimicrobial coatings against C. jejuni and Salmonella on fresh, refrigerated, vacuum-packed chicken breasts or L. monocytogenes on refrigerated, cured roast chicken. Malic acid improved the antimicrobial activity of Oriental mustard extract against L. monocytogenes, while EDTA improved its activity against Salmonella. Incorporation of 25 to 50 µl/g AITC or 100 to 250 mg/g Oriental mustard extract in 0.5%κ-carrageenan/2%chitosan coatings, prepared using 1.5% malic or acetic acid, reduced L. monocytogenes on cooked, cured, vacuum-packed chicken slices 4.2 to > 7.0 log10 CFU/g, compared to uncoated chicken by 70 d at 4 ºC. In addition, 0.2%κ-carrageenan/2%chitosan coatings (prepared using a 1% acetic acid solution) containing 250 mg/g mustard extract or 50 µl/g AITC reduced Salmonella numbers on vacuum-packed chicken breasts 3.0 log10 CFU/g by 21 d at 4 ºC. Further, 0.2%κ-carrageenan/2%chitosan coatings containing 50 or 100 µl/g AITC reduced numbers of C. jejuni on fresh, vacuum-packed chicken breasts > 5.0 log10 CFU/g (C. jejuni cells were not detected) after 5 d storage at 4 ºC, while coatings containing 200 to 300 mg/g Oriental mustard extract or 25 µl/g AITC reduced C. jejuni numbers by 3.6 to 4.6 log10 CFU/g. Numbers of lactic acid and aerobic bacteria on poultry meat products were significantly reduced by the coatings. It is clear that κ-carrageenan/chitosan coatings containing either AITC, mustard extract alone or combined with EDTA, malic or acetic acid significantly reduced C. jejuni and Salmonella on fresh, refrigerated, vacuum-packed chicken breasts and L. monocytogenes on refrigerated, cured roast chicken, and consequently enhanced their safety.

The world economy has changed dramatically over the last three decades in the twentieth century, especially in the areas of international trade and industrial organization. Within this new global configuration, markets have expanded and affected the industries organization and the value chain of companies, requiring new policies from countries to deal with changes in international environment. This thesis aims to analyze the Global Value Chain (GVC) of poultry meat produced by the agribusiness Mais Frango Miraguaí LTDA, in Rio Grande do Sul/Brazil, and to determine, from this company, the origin of their export competitiveness. Thus, the GVC of agribusiness Mais Frango Miraguaí Ltda was described; the stimuli for poultry breeders and for the slaugterhouse in GVC were determined; the competitive advantage of agribusiness in the production of poultry meat to international market was evaluated; and the effects of policies over financial costs in GVC were identified. For this, were analyzed the agribusiness GVC, through interviews with company managers, technical assistance and farmers, and data were collected considering production costs, referring to September 2015. The coordinator link of GVC is the slaugterhouse, adding to this upstream farmers and other input suppliers, and downstream the consumer market, domestic and external market. The competitiveness of the chain was analyzed by the Policy Analysis Matrix (PAM), which has proven its efficiency with Shawarma product produced (breast and boneless drumstick in a whole piece). The GVC is competitive, since it presents private prices and profits, it has comparative advantage, and the social profits are also positive, even the chain being aggravated by taxes and social charges. It was found that the captive coordination, through the existing integration agreements between the slaugterhouse and the producer, the use of high genetic standard, strict sanitary management, efficient management and product value added, are the main competitive elements of this chain. / A economia mundial mudou acentuadamente nas últimas três décadas do século XX, especialmente nas áreas de comércio internacional e organização industrial. Dentro dessa nova configuração global os mercados ampliaram e afetaram a organização das indústrias e a cadeia de valor das empresas, exigindo novas políticas dos países para fazer frente às alterações no ambiente internacional. Esta tese tem por objetivo analisar a Cadeia de Valor Global (CVG) da carne de frango produzida pela agroindústria Mais Frango Miraguaí Ltda., no Rio Grande do Sul, e determinar, a partir da empresa, a fonte de sua competitividade nas exportações. Desta forma foi descrita a CVG da agroindústria Mais Frango Miraguaí Ltda.; determinou-se os estímulos para os criadores de frango e para o frigorífico na CVG; foi avaliada a vantagem competitiva da agroindústria na produção de carne de frango para o mercado internacional; e identificou-se os efeitos das nos custos praticados na CVG. Para tal foram analisadas a CVG da agroindústria, através de entrevistas com os gestores da empresa, com a assistência técnica e com produtores rurais, e foram coletados dados relacionados aos custos de produção, referentes à setembro de 2015. O elo coordenador da CVG é o frigorífico, tendo este a montante os produtores rurais e demais fornecedores de insumos, e a jusante o mercado consumidor, no mercado interno e externo. A competitividade da cadeia foi analisada por meio da Matriz de Análise de Políticas (MAP), onde se comprovou sua eficiência com o produto Shawarma (peito e coxas desossadas em uma peça inteira) produzido pelo estabelecimento representativo. A CVG é competitiva, visto que esta apresenta lucros a preços privados e possui vantagem comparativa, sendo que os lucros sociais são também positivos, mesmo a cadeia sendo agravada pela carga tributária e encargos sociais. Constatou-se que a coordenação cativa, através dos contratos de integração existente entre o frigorífico e o produtor, a utilização de alto padrão genético, rigoroso manejo sanitário, gerenciamento eficiente e agregação de valor ao produto, são os principais elementos que constituem a competitividade desta cadeia.

Os consumidores estão cada vez mais conscientes e informados sobre a relação entre ingestão de sódio e o desenvolvimento da hipertensão. Desta forma, a indústria de alimentos vem desenvolvendo numerosos produtos com baixo teor de sódio para atender às necessidades deste novo perfil de consumidores. O presente projeto estudou os impactos tecnológicos, sensoriais e microbiológicos em salsichas com teor reduzido de sódio, adicionadas de fosfato e cloreto de potássio. Foram realizadas avaliações em duas matrizes cárneas: a) com carne bovina e suína e b) de carne mecanicamente separada (CMS) de frango. Na primeira etapa foram estudadas emulsões, na qual a otimização do processo utilizou como técnica o planejamento fatorial com delineamento composto central rotacional - DCCR, baseado na Metodologia de Superfície de Resposta, sendo as variáveis independentes: fosfato, cloreto de sódio e cloreto de potássio. Esta etapa foi realizada em sistema modelo obtendo as massas em mini cutter com todos os ingredientes previstos para obtenção de salsicha, variando os teores de fosfato, sal (NaCl) e cloreto de potássio (KCl). Na segunda etapa os melhores níveis de fosfato e KCl foram fixados e o cloreto de sódio continuou sendo avaliado em 3 níveis (1,00%, 1,30% e 1,75%), os tratamentos foram processados em planta piloto e realizados as análises físico-químicas, tecnológicas, microbiológicas e sensoriais. Os tratamentos foram monitorados ao longo de 56 dias nos produtos embalados à vácuo e estocados à 5 ± 2ºC em cinco períodos (1, 14, 28, 42 e 56 dias). Na emulsão de carne os melhores níveis de KCl e fosfato foram 0,85% e 0,25% respectivamente e na salsicha os tratamentos com 1,30 e 1,75% de NaCl apresentaram melhor desempenho na maioria das análises, principalmente na análise sensorial com consumidor. Já na emulsão de CMS os melhores níveis de KCl e fosfato foram 0,46% e 0,40%, respectivamente e na salsicha de CMS os tratamentos com 1,3% e 1,75% de NaCl apresentaram os melhores resultados principalmente na análise sensorial com consumidor. A redução da adição de 1,75% de NaCl para 1,3%, representa uma redução de cloreto de sódio de aproximadamente 25% e é viável do ponto de vista tecnológico, microbiológico e sensorial. / Consumers are increasingly aware and informed about the relationship between sodium intake and the development of hypertension with subsequent coronary problems. Thus, the food industry has developed numerous products with low sodium content to meet the needs of this new consumer profile. This project studied the technological, sensory, and microbiological impacts on low sodium sausages, with phosphate and potassium chloride added. Evaluations were performed in two meat matrices: a) beef and pork b) mechanically recovered poultry meat (MRPM). In the first stage, emulsions were studied, in which the optimization process used the technique of factorial design with a central composite design, based on Response Surface Methodology, and the independent variables were phosphate, sodium chloride and potassium chloride. This stage was performed in a model system obtaining the mass in a mini cutter with all the ingredients to obtain sausage with varying levels of phosphate, salt (NaCl) and potassium chloride (KCl). In the second stage, the best levels of phosphate and KCl were fixed and sodium chloride remained rated at three contents (1.00 %, 1.30 %, and 1.75 %). The treatments were processed in a pilot plant and the physical/chemical, technological, microbiological, and sensorial analyses were performed. The treatments were monitored over 56 days in vacuum packaged products and stored at 5 ± 2°C in five periods (1, 14, 28, 42, and 56 days). In meat emulsion, the best contents of KCl and phosphate were 0.85% and 0.25%, respectively. In sausage, the treatments with 1.30 and 1.75% NaCl showed the best performance in most analyses, mainly in the sensory analysis. In emulsion of mechanically recovered poultry meat, the best contents of KCl and phosphate were 0.46 % and 0.40 %, respectively, and sausage MRPM treatments with 1.3% and 1.75% NaCl showed the best results mainly in the sensory analysis. The reduction of 1.75% to 1.3% of NaCl represents a reduction of about 25% of sodium chloride and is viable in terms of technological, microbiological, and sensorial aspects.

A carne de frango apresenta vários problemas no processamento e conservação, sendo a oxidação lipídica um dos principais fatores limitantes da qualidade e aceitabilidade comercial deste produto. A indústria de alimentos busca desenvolver novas formulações que visem melhorar a qualidade e, principalmente, a segurança dos produtos alimentícios. Neste sentido, o uso de antioxidantes de fontes naturais, como os extratos de semente e casca de uva, mostra-se como uma alternativa segura e saudável para o processamento de carne de frango. O objetivo desse estudo foi caracterizar quimicamente extratos de semente e casca de uva das variedades Isabel e Niágara (Vitis labrusca) e determinar a atividade antioxidante e o efeito destes extratos sobre a estabilidade oxidativa e a qualidade da carne de frango processada e armazenada sob refrigeração (4±1ºC), em dois tipos de embalagem (aeróbica e a vácuo). Os compostos fenólicos dos extratos foram quantificados e a identificação feita pelo método da cromatografia líquida de alta eficiência (CLAE). A atividade antioxidante foi determinada pelo método do seqüestro do radical DPPH e pelo método da inibição da peroxidação lipídica. Primeiramente, carnes de frango processadas, contendo diferentes concentrações dos extratos (10, 20, 40 e 60 mg compostos fenólicos totais (CFT)) foram avaliadas em relação à estabilidade oxidativa. Posteriormente, as concentrações 40 e 60 mg CFT foram testadas, em embalagem aeróbica e a vácuo, sob refrigeração, por um período de 14 dias, sendo avaliadas a estabilidade oxidativa e a qualidade da carne. Os extratos de semente e casa de uva da variedade Niágara apresentaram os maiores teores de compostos fenólicos em relação os da variedade Isabel, sendo que pela técnica de CLAE foi possível identificar e quantificar os flavonóides catequina e epicatequina. Esses extratos apresentaram alta atividade antioxidante in vitro, sendo esta atividade atribuída à presença de compostos fenólicos. Os extratos de semente e casca de uva das variedades Isabel e Niágara (Vitis labrusca) demonstraram resultados satisfatórios quanto à estabilidade oxidativa, e quando aplicados nas concentrações de 40 a 60 mg CFT apresentaram resultados semelhantes ao antioxidante sintético BHT. A eficiência dos extratos de semente e casca de uva foi altamente dependente da concentração utilizada. A adição dos extratos de uva combinado com a embalagem a vácuo demonstrou ser uma boa técnica para aumentar a estabilidade lipídica na carne de frango cozida sob refrigeração, a 4±1ºC, num prazo de armazenamento de 14 dias. / Poultry meat has serious problems regarding processing and conservation. The lipid oxidation is one of the main factors limiting the quality and commercial acceptance of meat. The food industry has been trying to develop new formulations in order to improve the quality, and mainly, the safety of food products. Therefore, the use of natural antioxidants, such as grape seed and peel extracts, can be a safe and healthy alternative to poultry meat processing. The aim of this study was to chemically characterize grape seed and peel extracts from Isabel and Niagara varieties (Vitis labrusca) and to assess antioxidant activity and effect of these extracts on the quality and oxidative stability of processed and cold stored (4±1ºC) poultry meat, into two package types (aerobic and vacuum packaging). After quantified, the phenolic compounds found in the extracts were identified by the high-performance liquid chromatography (HPLC) method. The antioxidant activity was determined by the free radical scavenging activity on the DPPH method and by the linoleic acid peroxidation method. In a first essay, processed poultry meat, treated with different extracts concentrations (10, 20, 40 and 60 mg of total phenolic compounds (CFT)) were evaluated in relation to the oxidative stability. In sequence, the 40 and 60 mg CFT concentration extracts were tested over processed and cold stored poultry meat, packaged under aerobic and vacuum conditions, during 14 days; the poultry meat was then evaluated with regard to quality and oxidative stability. Due to the presence of catechins and epicatechin flavonoids, extracts from both grape varieties exhibited high in vitro activity. However, Niagara variety extract showed higher values of CFT when compared to Isabel variety. When applied to poultry meat, the grape seed and peel extracts from both varieties exhibited satisfactory results in relation to oxidative stability. Moreover, when applied with concentrations of 40 and 60 mg CFT, these extracts showed results similar to the ones found with BHT synthetic antioxidant. The grape extract efficiency showed high dependence of extract concentration. The addition of grape extracts combined with a vacuum package can be considered a good technique to raise lipid stability in cooked and cold stored (4±1ºC) poultry meat, until 14 days of storage.

A importância de toda a cadeia produtiva de aves no Brasil é fortemente impulsionada pela produção, exportação de carne de frango e pelo seu consumo per capita, que em 2009 foi de 11 milhões de toneladas, 3.3 milhões de toneladas e 40,1 Kg/hab/ano respectivamente. Porém a exportação de cortes e a preferência dos consumidores por cortes de frangos e filés ao invés dos frangos inteiros despertaram a necessidade de encontrar meios para o aproveitamento de dorsos, pescoços e ossos resultantes da desossa. A produção de carne mecanicamente separada com essas partes tornou-se ao longo dos anos uma alternativa viável, produzindo uma matéria-prima de baixo custo. O desenvolvimento de um produto reestruturado cozido congelado com carne de ave, com a utilização de pedaços desossados de peito e sobrecoxa, adicionados de carne mecanicamente separada do dorso da carcaça das aves tornou-se interessante para a indústria e consumidor. Porém as características desejadas pelo consumidor deste tipo de produto são consistência, força de adesão e suculência, ou seja, características de um filé de frango íntegro. Para conseguir essas características desejadas objetivou-se utilizar ingredientes não cárneos como a transglutaminase e albumina de ovo em pó, sendo esses ingredientes adicionais somados a adição de carne mecanicamente separada o estudo deste projeto. Para determinar a qualidade do produto foi realizada análise microbiológica no produto cru e após o cozimento e posteriormente para avaliar sua qualidade durante o armazenamento e sua vida útil a -18°C, foram realizadas análises como pH, cor instrumental, valor de TBARS e análise sensorial, sendo esta última para determinar aceitação do produto por consumidores. / The importance poultry production chain in Brazil is strongly stimulated by meat poultry exportation 11 million of tons, 3,3 million of tons and by per capita consumption 40,1 kg/hab/year. Although the preference of exportation cuts and the consumer preference by cuts instead of whole chicken so theres a search for the use of backs, necks, and bones from the boning. The mechanically separated meat production with that parts over the years one viable alternative, resulting in low cost raw material. Then the develop of restructured product frozen and cooked with poultry meat, the utilization of boning meat from chest and thighs , added to most from back bones is extremity interesting for industry and consumers. But the features desired by consumers for this product are the cohesiveness, richness and strength of accession, that means whole chicken characteristics. To achieve these desired features aimed to use non meat ingredients as transglutaminase and egg albumin powder were used, and these non meat ingredients added to the minced meat in the objective of this project. To determine the quality of the product, microbiological analyses in the raw product and after cooking were performed, and later, to evaluate its quality during storage and service life at -18°C, analyses such as pH, instrumental color, TBARS values and analysis sensory were performed, the latter being performed to determine the acceptance of the product by consumers.

Made available in DSpace on 2014-08-20T14:37:50Z (GMT). No. of bitstreams: 1 dissertacao_daiani_teixeira_da_silva.pdf: 375746 bytes, checksum: 539868549fb7e9d324125c3f8040cb19 (MD5) Previous issue date: 2012-07-12 / Campylobacter is a bacteria related to foodborne illnesses. It is one of the most common causes of diarrhea in humans in the world. It might cause Guillain-Barre syndrome, which attacks the cells of the peripheral nervous system causing muscle paralysis. Some strains of this bacterium can produce a cytolethal distending toxin (CDT), which can exacerbate diarrheal diseases. Birds are the main reservoir of this micro-organism, of which they are asymptomatic hosts. During the slaughter of broilers, contamination of meat and its products may occur. They are the main responsible for infections in humans. The objective of this study was to verify the presence of C. jejuni and C. coli in poultry meat, chicken and human feces and investigate the presence of cdtA, cdtB and cdtC genes in the isolates. A hundred chicken fecal samples, a hundred samples of retail poultry products and a hundred samples of human feces were collected. The poultry products were washed with Brucella broth and spread onto plates of Columbia agar. The feces samples were directly spread onto plates containing this medium. Confirmation of isolates and species identification were performed by PCR. PCR was also used to investigate the presence of cdt genes. Campylobacter was found in 61% of the chicken fecal samples, in 20% of the poultry products and in 3% of the human feces. C. jejuni was the predominant species and 93,5% of them exhibited the cdt genes. Despite the fact that the prevalence of Campylobacter found in human feces was low, the occurrence of Campylobacter in broilers and poultry products sold in southern Rio Grande do Sul was high. Besides, it has been observed that most of the isolates were highly likely to produce CDT. These results suggest there is a need for preventive measures in the production system and good manufacturing practices in the industry so as to minimize contamination of products and reduce the risk to consumers. / Campylobacter é uma bactéria relacionada a doenças transmitidas por alimentos. É uma das causas mais comuns de diarreia em humanos em todo o mundo. Raramente, pode causar a Síndrome de Guillain-Barré, que ataca as células do sistema nervoso periférico, causando paralisia muscular. Algumas cepas desta bactéria podem produzir uma toxina citoletal distensiva (CDT) capaz de agravar os quadros diarreicos. As aves são o principal reservatório do micro-organismo, do qual são portadoras assintomáticas. Durante o abate de frangos de corte pode ocorrer a contaminação da carne e seus produtos, que são os principais responsáveis pelas infecções em humanos. O objetivo do trabalho foi verificar a presença de C. jejuni e C. coli em carne de frango, fezes de frango e humanas e pesquisar a presença dos genes cdtA, cdtB e cdtC nos isolados. Foram coletadas 100 amostras de conteúdo fecal de aves de corte, 100 de produtos de frango no comércio varejista e 100 de fezes de humanos. Os produtos de frango foram lavados com Caldo Brucella e semeados em placas de Agar Columbia. As amostras de fezes foram diretamente semeadas nas placas com este meio. A confirmação dos isolados e a identificação das espécies foram realizadas através da técnica de PCR. Também foi utilizada a PCR para pesquisar a presença dos genes cdt. Obteve-se 61% de amostras de fezes de frango positivas para Campylobacter, 20% de produtos de frango e 3% de fezes de humanos. A espécie C.jejuni foi predominante e 93,5% dos isolados desta espécie apresentaram os genes para a toxina CDT. Apesar da prevalência de Campylobacter observada em fezes de humanos ter sido baixa, a ocorrência em fezes de frangos de corte e produtos de frango comercializados na região Sul do Rio Grande do Sul foi elevada e a maioria dos isolados apresentou potencial para a produção da toxina CDT. Esses resultados são indicativos da necessidade de medidas preventivas no sistema de produção e de boas práticas de fabricação na indústria, de forma a minimizar a contaminação dos produtos e diminuir o risco para os consumidores.

A carne de frango apresenta vários problemas no processamento e conservação, sendo a oxidação lipídica um dos principais fatores limitantes da qualidade e aceitabilidade comercial deste produto. A indústria de alimentos busca desenvolver novas formulações que visem melhorar a qualidade e, principalmente, a segurança dos produtos alimentícios. Neste sentido, o uso de antioxidantes de fontes naturais, como os extratos de semente e casca de uva, mostra-se como uma alternativa segura e saudável para o processamento de carne de frango. O objetivo desse estudo foi caracterizar quimicamente extratos de semente e casca de uva das variedades Isabel e Niágara (Vitis labrusca) e determinar a atividade antioxidante e o efeito destes extratos sobre a estabilidade oxidativa e a qualidade da carne de frango processada e armazenada sob refrigeração (4±1ºC), em dois tipos de embalagem (aeróbica e a vácuo). Os compostos fenólicos dos extratos foram quantificados e a identificação feita pelo método da cromatografia líquida de alta eficiência (CLAE). A atividade antioxidante foi determinada pelo método do seqüestro do radical DPPH e pelo método da inibição da peroxidação lipídica. Primeiramente, carnes de frango processadas, contendo diferentes concentrações dos extratos (10, 20, 40 e 60 mg compostos fenólicos totais (CFT)) foram avaliadas em relação à estabilidade oxidativa. Posteriormente, as concentrações 40 e 60 mg CFT foram testadas, em embalagem aeróbica e a vácuo, sob refrigeração, por um período de 14 dias, sendo avaliadas a estabilidade oxidativa e a qualidade da carne. Os extratos de semente e casa de uva da variedade Niágara apresentaram os maiores teores de compostos fenólicos em relação os da variedade Isabel, sendo que pela técnica de CLAE foi possível identificar e quantificar os flavonóides catequina e epicatequina. Esses extratos apresentaram alta atividade antioxidante in vitro, sendo esta atividade atribuída à presença de compostos fenólicos. Os extratos de semente e casca de uva das variedades Isabel e Niágara (Vitis labrusca) demonstraram resultados satisfatórios quanto à estabilidade oxidativa, e quando aplicados nas concentrações de 40 a 60 mg CFT apresentaram resultados semelhantes ao antioxidante sintético BHT. A eficiência dos extratos de semente e casca de uva foi altamente dependente da concentração utilizada. A adição dos extratos de uva combinado com a embalagem a vácuo demonstrou ser uma boa técnica para aumentar a estabilidade lipídica na carne de frango cozida sob refrigeração, a 4±1ºC, num prazo de armazenamento de 14 dias. / Poultry meat has serious problems regarding processing and conservation. The lipid oxidation is one of the main factors limiting the quality and commercial acceptance of meat. The food industry has been trying to develop new formulations in order to improve the quality, and mainly, the safety of food products. Therefore, the use of natural antioxidants, such as grape seed and peel extracts, can be a safe and healthy alternative to poultry meat processing. The aim of this study was to chemically characterize grape seed and peel extracts from Isabel and Niagara varieties (Vitis labrusca) and to assess antioxidant activity and effect of these extracts on the quality and oxidative stability of processed and cold stored (4±1ºC) poultry meat, into two package types (aerobic and vacuum packaging). After quantified, the phenolic compounds found in the extracts were identified by the high-performance liquid chromatography (HPLC) method. The antioxidant activity was determined by the free radical scavenging activity on the DPPH method and by the linoleic acid peroxidation method. In a first essay, processed poultry meat, treated with different extracts concentrations (10, 20, 40 and 60 mg of total phenolic compounds (CFT)) were evaluated in relation to the oxidative stability. In sequence, the 40 and 60 mg CFT concentration extracts were tested over processed and cold stored poultry meat, packaged under aerobic and vacuum conditions, during 14 days; the poultry meat was then evaluated with regard to quality and oxidative stability. Due to the presence of catechins and epicatechin flavonoids, extracts from both grape varieties exhibited high in vitro activity. However, Niagara variety extract showed higher values of CFT when compared to Isabel variety. When applied to poultry meat, the grape seed and peel extracts from both varieties exhibited satisfactory results in relation to oxidative stability. Moreover, when applied with concentrations of 40 and 60 mg CFT, these extracts showed results similar to the ones found with BHT synthetic antioxidant. The grape extract efficiency showed high dependence of extract concentration. The addition of grape extracts combined with a vacuum package can be considered a good technique to raise lipid stability in cooked and cold stored (4±1ºC) poultry meat, until 14 days of storage.